SMED30028190

Overview
NameSMED30028190
Smed IDSMED30028190
Length (bp)749
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30028190 (SMED30028190) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30028190 (SMED30028190) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30028190

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30028190h1SMcG0019999 SmedASXL_007419SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30028190h1SMcG0019999 dd_Smed_v4_18162_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30028190h1SMcG0019999 dd_Smed_v4_18162_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30028190h1SMcG0019999 dd_Smed_v4_18162_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30028190h1SMcG0019999 dd_Smed_v4_18162_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30028190h1SMcG0019999 dd_Smed_v6_18162_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30028190 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.018320:1..631 -3085
Homology
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BLAST of SMED30028190 vs. Planmine SMEST
Match: SMESG000035239.1 (SMESG000035239.1)

HSP 1 Score: 382.874 bits (982), Expect = 1.079e-136
Identity = 192/195 (98.46%), Postives = 193/195 (98.97%), Query Frame = 3 
Query:   48 MTLSNCFPFKKNRRTDLNLMDENVCNQTVPLTDDRENMEIIVPTDQQVTNQSLPIKSESSVTPNRKPMRRETLLRLSKKTNPELINQYDIDSLIRMSVNKQRRQSWEFKVRNSIRLTSKDLTEVRKKLSLANKDGIITEKTNKTNSENDESILLKHGDYENIDSEINADNDPSHLNGSIKQTNNEEIISENLKEK 632
            MTLSNCFPFKKNRRTDLNLMDENVCNQTVPLTDDRENMEIIVPTDQQVTNQSLPIKSESSVT NRK MRRETLLRLSKKTNPELINQYDIDSLIRMSVNKQRRQSWEFKVRNSIRLTSKDLTEVRKKLSLANKDGIITEKTNKTNSENDESILLKHGDYENIDSEINAD+DPSHLNGSIKQTNNEEIISENLKEK
Sbjct:    1 MTLSNCFPFKKNRRTDLNLMDENVCNQTVPLTDDRENMEIIVPTDQQVTNQSLPIKSESSVTLNRKHMRRETLLRLSKKTNPELINQYDIDSLIRMSVNKQRRQSWEFKVRNSIRLTSKDLTEVRKKLSLANKDGIITEKTNKTNSENDESILLKHGDYENIDSEINADDDPSHLNGSIKQTNNEEIISENLKEK 195
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BLAST of SMED30028190 vs. Planmine SMEST
Match: SMESG000035240.1 (SMESG000035240.1)

HSP 1 Score: 135.576 bits (340), Expect = 9.542e-41
Identity = 72/91 (79.12%), Postives = 72/91 (79.12%), Query Frame = -2 
Query:  185 IIPSLFANESFFLTSVKSLDVNRIEFRTLNSQDCRRCLLTDIRIKLSMSYWLMSSGFVFFDSLKRVSRRIGFLFGVTEDSDLIGRDWFVTC 457
            IIPSLFANESFFLTSVKSLDVNRIEFRTLNSQDCRRCLLTDIRIKLSMSYWLMSS                   GVTEDSDLIGRDWFVTC
Sbjct:   16 IIPSLFANESFFLTSVKSLDVNRIEFRTLNSQDCRRCLLTDIRIKLSMSYWLMSS-------------------GVTEDSDLIGRDWFVTC 87
The following BLAST results are available for this feature:
BLAST of SMED30028190 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028190 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028190 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028190 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028190 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028190 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028190 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028190 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028190 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028190 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028190 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028190 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028190 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028190 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000035239.11.079e-13698.46SMESG000035239.1[more]
SMESG000035240.19.542e-4179.12SMESG000035240.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028190 ID=SMED30028190|Name=SMED30028190|organism=Schmidtea mediterranea sexual|type=transcript|length=749bp
TTAATCGAAATTGTTATTGACATAAGTTTGAATAATTATCATATATAATG
ACTCTGTCGAATTGCTTTCCTTTTAAAAAAAATCGCCGAACCGATTTGAA
TTTAATGGACGAAAACGTTTGCAACCAAACGGTACCTTTAACTGATGACA
GAGAAAACATGGAAATTATTGTCCCAACGGATCAGCAAGTGACAAACCAA
TCTCTTCCAATCAAATCAGAATCCTCAGTAACACCAAATAGAAAACCAAT
GCGCCGTGAAACTCTTTTGAGACTATCGAAAAAAACAAACCCGGAACTCA
TTAACCAATATGACATCGATAGCTTGATCCGAATGTCTGTCAATAAACAA
CGCCGACAATCTTGGGAATTTAAAGTCCGAAATTCTATTCGATTGACATC
CAAAGATTTGACAGAAGTGAGAAAAAAGCTTTCATTGGCAAATAAAGACG
GAATTATAACAGAGAAAACTAATAAAACCAATTCCGAAAACGATGAAAGC
ATTTTACTGAAGCACGGTGACTATGAGAATATAGATTCCGAAATTAATGC
TGACAATGATCCAAGCCACTTAAATGGTTCGATAAAGCAAACAAACAATG
AAGAAATAATTTCTGAAAATCTCAAAGAAAAATCAACGAATTAAACACTA
TCAATCAATCTAAACCTCACAATAAACCATTCAGAAGATATAAAAATCTT
ATTTAACTAAACCATGAATTTTATTGTAGTGTTACCCAAGTGGGTATTT
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protein sequence of SMED30028190-orf-1

>SMED30028190-orf-1 ID=SMED30028190-orf-1|Name=SMED30028190-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=199bp
MTLSNCFPFKKNRRTDLNLMDENVCNQTVPLTDDRENMEIIVPTDQQVTN
QSLPIKSESSVTPNRKPMRRETLLRLSKKTNPELINQYDIDSLIRMSVNK
QRRQSWEFKVRNSIRLTSKDLTEVRKKLSLANKDGIITEKTNKTNSENDE
SILLKHGDYENIDSEINADNDPSHLNGSIKQTNNEEIISENLKEKSTN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000099neuron
PLANA:0000418head
PLANA:0000420parapharyngeal region