SAM-dependent methyltransferase

Overview
NameSAM-dependent methyltransferase
Smed IDSMED30028146
Length (bp)770
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SAM-dependent methyltransferase (SMED30028146) t-SNE clustered cells

Violin plots show distribution of expression levels for SAM-dependent methyltransferase (SMED30028146) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SAM-dependent methyltransferase (SMED30028146) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SAM-dependent methyltransferase (SMED30028146) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30028146

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30028146h1SMcG0003248 dd_Smed_v4_5458_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30028146h1SMcG0003248 dd_Smed_v4_5458_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SAM-dependent methyltransferase vs. Ensembl Human
Match: METTL27 (methyltransferase like 27 [Source:HGNC Symbol;Acc:HGNC:19068])

HSP 1 Score: 65.855 bits (159), Expect = 3.584e-12
Identity = 46/175 (26.29%), Postives = 78/175 (44.57%), Query Frame = 2 
Query:  101 NITNFIELENLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLRHSYITTHQKEII-PEIERLNKENV 622
             I +  +  + Y+ W+  YD+D++        + V  L         S  ILD+  GTGLVA ELR  G+  +  ++ +  M+ +A+   LY       L   EP+ +  E T+D ++  G  S   +    I P L + K GGL   + R +      KE +   ++RL +  +
Sbjct:   21 GIPDLAQKLHFYDRWAPDYDQDVATLLYRAPRLAVDCLTQALPGPPHSALILDVACGTGLVAAELRAPGFLQLHGVDGSPGMLEQAQAPGLYQRLSLCTLG-QEPLPS-PEGTFDAVLIVGALSDGQVPCNAI-PELHVTKPGGLVCLTTRTNSSNLQYKEALEATLDRLEQAGM 192
[Close]
BLAST of SAM-dependent methyltransferase vs. Ensembl Zebrafish
Match: mettl27 (methyltransferase like 27 [Source:ZFIN;Acc:ZDB-GENE-060421-5918])

HSP 1 Score: 63.1586 bits (152), Expect = 1.754e-11
Identity = 39/163 (23.93%), Postives = 77/163 (47.24%), Query Frame = 2 
Query:  128 NLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNE-PVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLRHSYITTHQKEIIPEIERLNK 613
              Y+ W+  Y++D++  +     +    +   F+   +   +LD+  GTGLV++ L+++G+ + D ++ +  M+  AK   LY       L  +  PVK    +TYD +I  G  S   +  + I+ +    K GG    + R +   T  ++   E+E++ K
Sbjct:   36 GFYDTWADNYEQDVAVLDYRAPLLAAECVSSFFNDDREKATVLDVACGTGLVSKHLKRMGFRHFDGVDGSLRMLEGAKKTGLYKQLMHCMLGQDRIPVKA---ETYDVVIIVGALSVGQVPLKVIRELWDATKPGGYVCMTTRAN---TDNQKYKAELEQMIK 192
[Close]
BLAST of SAM-dependent methyltransferase vs. Ensembl Xenopus
Match: vegfb (vascular endothelial growth factor B [Source:Xenbase;Acc:XB-GENE-483494])

HSP 1 Score: 64.6994 bits (156), Expect = 6.966e-12
Identity = 36/142 (25.35%), Postives = 63/142 (44.37%), Query Frame = 2 
Query:  128 NLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLR 553
            + Y+ W+  Y+ED+S       ++    L        +S+ +LD+  GTGL A+EL   G+     ++ +  M+  A+ K LYL   +  L   +       D Y+ +I  G  S   +    I  +L++ K GG    + R
Sbjct:   29 HFYDHWAPEYEEDVSVLEYKAPYLAAYTLASAIGSNRESQLVLDVACGTGLAAQELHHCGFRLFHGVDGSTGMLQVAETKELYLELKQCML--GQESLPFPSDLYNAVIIVGALSDGQVPVSVIPELLRVTKQGGFVCLTTR 168
[Close]
BLAST of SAM-dependent methyltransferase vs. Ensembl Mouse
Match: Mettl27 (methyltransferase like 27 [Source:MGI Symbol;Acc:MGI:1933146])

HSP 1 Score: 69.3218 bits (168), Expect = 9.591e-14
Identity = 41/151 (27.15%), Postives = 70/151 (46.36%), Query Frame = 2 
Query:  101 NITNFIELENLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLR 553
             IT+       Y++W+  YD+D++        + V  L   F        ILD+  GTGLVA EL+  G+  +  ++ +  M+ +A+ + LY ++        EP+    E T+D +I  G  S   +    I  +L++ K GGL   + R
Sbjct:   21 GITDLACKLRFYDDWAPEYDQDVAALKYRAPRLAVDCLSRAFRGSPHDALILDVACGTGLVAVELQARGFLQVQGVDGSPEMLKQARARGLY-HHLSLCTLGQEPLPD-PEGTFDAVIIVGALSEGQVPCSAIPELLRVTKPGGLVCLTTR 169
[Close]
BLAST of SAM-dependent methyltransferase vs. Ensembl Mouse
Match: Mettl27 (methyltransferase like 27 [Source:MGI Symbol;Acc:MGI:1933146])

HSP 1 Score: 69.3218 bits (168), Expect = 1.210e-13
Identity = 41/152 (26.97%), Postives = 70/152 (46.05%), Query Frame = 2 
Query:   98 QNITNFIELENLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLR 553
              IT+       Y++W+  YD+D++        + V  L   F        ILD+  GTGLVA EL+  G+  +  ++ +  M+ +A+ + LY ++        EP+    E T+D +I  G  S   +    I  +L++ K GGL   + R
Sbjct:   20 HGITDLACKLRFYDDWAPEYDQDVAALKYRAPRLAVDCLSRAFRGSPHDALILDVACGTGLVAVELQARGFLQVQGVDGSPEMLKQARARGLY-HHLSLCTLGQEPLPD-PEGTFDAVIIVGALSEGQVPCSAIPELLRVTKPGGLVCLTTR 169
[Close]
BLAST of SAM-dependent methyltransferase vs. Ensembl Mouse
Match: Mettl27 (methyltransferase like 27 [Source:MGI Symbol;Acc:MGI:1933146])

HSP 1 Score: 69.3218 bits (168), Expect = 1.210e-13
Identity = 41/152 (26.97%), Postives = 70/152 (46.05%), Query Frame = 2 
Query:   98 QNITNFIELENLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLR 553
              IT+       Y++W+  YD+D++        + V  L   F        ILD+  GTGLVA EL+  G+  +  ++ +  M+ +A+ + LY ++        EP+    E T+D +I  G  S   +    I  +L++ K GGL   + R
Sbjct:   20 HGITDLACKLRFYDDWAPEYDQDVAALKYRAPRLAVDCLSRAFRGSPHDALILDVACGTGLVAVELQARGFLQVQGVDGSPEMLKQARARGLY-HHLSLCTLGQEPLPD-PEGTFDAVIIVGALSEGQVPCSAIPELLRVTKPGGLVCLTTR 169
[Close]
BLAST of SAM-dependent methyltransferase vs. Ensembl Mouse
Match: Mettl27 (methyltransferase like 27 [Source:MGI Symbol;Acc:MGI:1933146])

HSP 1 Score: 69.3218 bits (168), Expect = 1.210e-13
Identity = 41/152 (26.97%), Postives = 70/152 (46.05%), Query Frame = 2 
Query:   98 QNITNFIELENLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLR 553
              IT+       Y++W+  YD+D++        + V  L   F        ILD+  GTGLVA EL+  G+  +  ++ +  M+ +A+ + LY ++        EP+    E T+D +I  G  S   +    I  +L++ K GGL   + R
Sbjct:   20 HGITDLACKLRFYDDWAPEYDQDVAALKYRAPRLAVDCLSRAFRGSPHDALILDVACGTGLVAVELQARGFLQVQGVDGSPEMLKQARARGLY-HHLSLCTLGQEPLPD-PEGTFDAVIIVGALSEGQVPCSAIPELLRVTKPGGLVCLTTR 169
[Close]
BLAST of SAM-dependent methyltransferase vs. Ensembl Mouse
Match: Mettl27 (methyltransferase like 27 [Source:MGI Symbol;Acc:MGI:1933146])

HSP 1 Score: 53.5286 bits (127), Expect = 1.088e-8
Identity = 26/92 (28.26%), Postives = 45/92 (48.91%), Query Frame = 2 
Query:  101 NITNFIELENLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLY 376
             IT+       Y++W+  YD+D++        + V  L   F        ILD+  GTGLVA EL+  G+  +  ++ +  M+ +A+ + LY
Sbjct:   21 GITDLACKLRFYDDWAPEYDQDVAALKYRAPRLAVDCLSRAFRGSPHDALILDVACGTGLVAVELQARGFLQVQGVDGSPEMLKQARARGLY 112
[Close]
BLAST of SAM-dependent methyltransferase vs. UniProt/SwissProt
Match: sp|Q8N6F8|MET27_HUMAN (Methyltransferase-like protein 27 OS=Homo sapiens OX=9606 GN=METTL27 PE=1 SV=2)

HSP 1 Score: 65.855 bits (159), Expect = 1.721e-11
Identity = 46/175 (26.29%), Postives = 78/175 (44.57%), Query Frame = 2 
Query:  101 NITNFIELENLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLRHSYITTHQKEII-PEIERLNKENV 622
             I +  +  + Y+ W+  YD+D++        + V  L         S  ILD+  GTGLVA ELR  G+  +  ++ +  M+ +A+   LY       L   EP+ +  E T+D ++  G  S   +    I P L + K GGL   + R +      KE +   ++RL +  +
Sbjct:   21 GIPDLAQKLHFYDRWAPDYDQDVATLLYRAPRLAVDCLTQALPGPPHSALILDVACGTGLVAAELRAPGFLQLHGVDGSPGMLEQAQAPGLYQRLSLCTLG-QEPLPS-PEGTFDAVLIVGALSDGQVPCNAI-PELHVTKPGGLVCLTTRTNSSNLQYKEALEATLDRLEQAGM 192
[Close]
BLAST of SAM-dependent methyltransferase vs. TrEMBL
Match: A0A433U3L9 (Methyltransf_11 domain-containing protein OS=Elysia chlorotica OX=188477 GN=EGW08_003774 PE=4 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 7.233e-30
Identity = 73/225 (32.44%), Postives = 126/225 (56.00%), Query Frame = 2 
Query:   50 SASENDKGIVYIDHKI--QNITNFIELENLYNEWSKTYDEDL-SKPNSYCGFIMVSKLLD-LFSFRDK-SRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLRHSYI---TTHQKEIIPEIERLNKENVIKIRMLSEIPDYYPGVPAFGIVLEKV 700
            ++++N +    ++ KI  ++IT + E++  Y++W+  YD+ L  KP  Y   +   + +D LF   +K +RR LD+GAGTGL  E L K+GY NID LE N  M    K K ++  +Y + +  + P+KT  +++YD I  AG F+P H+    I  +++LLK GG F+  +R  +I     ++  ++P    L K+ +IK    +  P++Y   P   +V +K+
Sbjct:    5 TSAKNFRDSEQVEQKIFRRDIT-YQEVQEQYDDWADYYDQTLDGKPKVYNTLMAYEEGMDTLFDATEKPNRRCLDLGAGTGLTGERLLKVGYTNIDGLEPNEKMADVCKKKGIFREFYVQPIGGDNPLKT-PDNSYDAICCAGSFAPGHIPCSAIPEIVRLLKPGGYFLNCMREEFIHDVPEYKDRLVPMFNDLEKQGLIKTVEWTVYPNHYIDKPGVRMVYQKL 227
[Close]
BLAST of SAM-dependent methyltransferase vs. TrEMBL
Match: C3ZBD1 (Methyltransf_25 domain-containing protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_65009 PE=4 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 5.182e-29
Identity = 61/154 (39.61%), Postives = 92/154 (59.74%), Query Frame = 2 
Query:  119 ELENLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLRHSYITTHQK 580
            E +  Y+ W+  YD+DLS P  Y G   V++ L       K  RILD  AGTGLV EELRKL ++NID+L++N  M+  AK KN+Y N  ++FL PN     + +DTYD +  AG FS  HL    ++ M++++K GG+    ++ +++  + K
Sbjct:   24 ESQAFYSTWAPEYDKDLSTPGLYKGPSQVAEALAGVLRDRKDARILDAAAGTGLVGEELRKLEFSNIDALDANKEMLDIAKTKNVYRNLIQDFLGPNR--LQVADDTYDALCCAGSFSDGHLKCDCLEEMIRIVKPGGMICLIVKEAFLLEYGK 175
[Close]
BLAST of SAM-dependent methyltransferase vs. TrEMBL
Match: A0A1I8IBM4 (MTS domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001749g1 PE=4 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 1.224e-28
Identity = 63/195 (32.31%), Postives = 115/195 (58.97%), Query Frame = 2 
Query:  122 LENLYNEWSKTYDEDL-SKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLRHSYI-TTHQKEIIPEIERLNKENVIKIRMLSEIPDYYPGVPAFGIVLEKV 700
            ++++Y+ WS +Y+ DL +  + Y     ++++ D    +DK+ RILD+ AGTGL    L++LG+ NID+++ +  M+  A  K +Y N +EE +  ++ VK++++ +YD I+ +G F P HL   H+KP+   LKSGGLF  ++R  Y+ T +  ++ P ++R+  E + +      +  YY  +P    V  +V
Sbjct:   32 VKSMYDSWSGSYELDLLNMSHGYTHPQKIARVADTH-LKDKNARILDVCAGTGLGGVALKELGFTNIDAIDGSRGMLDLAAKKQIYKNLFEELI-GSDSVKSVEQASYDAIVTSGSFLPGHLGPMHLKPLALTLKSGGLFCMAMREEYLRTDYLLDLEPTMDRMEAEGIWQKVQKGIMEHYYNDLPGVFYVYRRV 224
[Close]
BLAST of SAM-dependent methyltransferase vs. TrEMBL
Match: A0A267EL55 (Methyltransf_25 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig007718g1 PE=4 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 2.089e-28
Identity = 59/185 (31.89%), Postives = 108/185 (58.38%), Query Frame = 2 
Query:  131 LYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFS--FRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLRHSYI-TTHQKEIIPEIERLNKENVIKIRMLSEIPDYYPGVPA 676
            LY++WS  Y+++     S  G+    ++ D+ +    DK+ RILD+ AGTGL    L+K G++NID+L+ +A M+ KA+ K +Y   ++E +    P+  +++  YD I+ +G F P HL    ++ +   LK  GLF  ++R +Y+ T + +++ PE+ER+ +E + KI     + +Y+ G+  
Sbjct:   33 LYSDWSDEYEKNFLNMTS--GYEHPERIADIAASVLPDKTCRILDVCAGTGLGGAALKKRGFDNIDALDGSAGMLEKARQKGIYSCIFQEVIGTG-PLTKVEKGAYDAIVTSGSFVPGHLGPGQLRVLAGTLKPNGLFCMAMREAYLETDYLRDLQPEMERMQQEGIWKIERCDRMDNYHNGLSG 214
[Close]
BLAST of SAM-dependent methyltransferase vs. TrEMBL
Match: A0A267DED2 (Methyltransf_25 domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig014729g2 PE=4 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 2.451e-28
Identity = 59/185 (31.89%), Postives = 108/185 (58.38%), Query Frame = 2 
Query:  131 LYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFS--FRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLRHSYI-TTHQKEIIPEIERLNKENVIKIRMLSEIPDYYPGVPA 676
            LY++WS  Y+++     S  G+    ++ D+ +    DK+ RILD+ AGTGL    L+K G++NID+L+ +A M+ KA+ K +Y+   +E +    P+  +++  YD I+ +G F P HL    ++ +   LK  GLF  ++R +Y+ T + +++ PE+ER+ +E + KI     + +Y+ G+  
Sbjct:   33 LYSDWSDEYEKNFLNMTS--GYEHPERIADIAASVLPDKTCRILDVCAGTGLGGAALKKRGFDNIDALDGSAGMLEKARQKGIYICIVQEVIGTG-PLTKVEKGAYDAIVTSGSFVPGHLGPGQLRVLAGTLKPNGLFCMAMREAYLETDYLRDLQPEMERMQQEGIWKIERCDRMDNYHNGLSG 214
[Close]
BLAST of SAM-dependent methyltransferase vs. Ensembl Cavefish
Match: mettl27 (methyltransferase like 27 [Source:ZFIN;Acc:ZDB-GENE-060421-5918])

HSP 1 Score: 62.3882 bits (150), Expect = 2.538e-11
Identity = 40/164 (24.39%), Postives = 80/164 (48.78%), Query Frame = 2 
Query:  128 NLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRI-LDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLRHSYITTHQKEIIPEIERLNKE 616
            + YN W++ Y++D++  + Y   ++ ++ +      DK R I LD+  GTGLV+  L+++G+     ++ +  M+  A+   LYL   +  L   +P   +Q +TYD ++  G  S   +    ++ + ++ K GG    + R +      K  +  + R  KE
Sbjct:   31 SFYNTWAENYEQDVALLD-YRAPLLAAECITSHFKGDKERAIILDVACGTGLVSAHLKRMGFCQFIGVDGSEGMLDLARKTGLYLELMQCMLG-QDPFP-VQNETYDIVVIVGALSVGQVPVAVLRELWQVTKPGGYVCMTTRGNADNQEYKTELEHMMRTMKE 191
[Close]
BLAST of SAM-dependent methyltransferase vs. Ensembl Nematostella
Match: EDO35583 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SL24])

HSP 1 Score: 83.9593 bits (206), Expect = 2.019e-19
Identity = 52/159 (32.70%), Postives = 85/159 (53.46%), Query Frame = 2 
Query:   92 KIQNITNFIELENLYNEWSKTYDEDLSKPNSYCG----FIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSR-HIKPMLKLLKSGGLFITSLR 553
            K  +  N  EL + Y+E +  YD+ +     Y G      + +K L    F +  R ILD+G+GTGL AE L K G+ NID+L+ +      A+ KNLY NY  ++LEP   +  I +  YD +   GVF+  H+ +   +  M++++++ GL   ++R
Sbjct:   38 KCNDFDNLKELVSYYDETAIHYDKYVKDARGYIGPEVAASITAKFLKSQGFSEDCR-ILDVGSGTGLQAEGLVKHGFTNIDALDPSEKSNEVARKKNLYKNYITDYLEPGRKL-NISDGDYDAVACVGVFTKGHVTAEAAMDEMVRVVRAEGLVCFTIR 194
[Close]
BLAST of SAM-dependent methyltransferase vs. Ensembl Nematostella
Match: EDO45905 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRY0])

HSP 1 Score: 82.4185 bits (202), Expect = 4.239e-19
Identity = 47/163 (28.83%), Postives = 83/163 (50.92%), Query Frame = 2 
Query:   92 KIQNITNFIELENLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDK--------SRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLRH 556
            ++ N T   E+  +Y+ W+  Y+ D    NS+ G++      D+F             S R++D GAGTG+V E L++LGY N+D L+ +  M+  A+ K +Y N     L   E +  I +  Y+ I+ +G  + AH+ +  +  + + LK GG+   ++R 
Sbjct:   12 QVSNQTTEAEIRQIYDNWATNYEMD---TNSH-GYVAHKTCTDVFDAEMAKAYPKYRLSLRVMDAGAGTGIVGEHLQQLGYTNVDGLDISQEMLNIARQKRVYKNLICAGLSETE-IAEIADGQYEGIVCSGTITVAHVKAASLGELRRWLKPGGIICFTIRQ 169
[Close]
BLAST of SAM-dependent methyltransferase vs. Ensembl Nematostella
Match: EDO42150 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2H3])

HSP 1 Score: 73.9442 bits (180), Expect = 5.521e-16
Identity = 54/191 (28.27%), Postives = 96/191 (50.26%), Query Frame = 2 
Query:  122 LENLYNEWSKTYDEDLS-----KPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLRHSYITTHQKEIIPEIERLNKENVIKIRMLSE-IPDYYPGVPA 676
            ++ +Y++W+  Y++D++       ++Y   I+ S L  LF    +S  ILD GAGTGL+    ++LGY  ID+L+ +  M+  AK KN+Y       +   +    I +D YD +I  G  S  ++ +  +  ++  +K GG+   +L        + E    I+ L +E   K R+ S+ + DY   +PA
Sbjct:   22 VKKVYDDWALDYEKDIAIRGFDSHHTY-ARILDSTLATLFPGNHQSLHILDAGAGTGLMGVSFKRLGYTIIDALDFSQEMLNCAKEKNVYRRLICAPIGEGKH-SDISQDNYDVLICTGTLSSPNVRASGLDELIHYVKPGGVICFTLHLHLYDNRESEYRKRIDELEREG--KWRLHSKHVTDYNKYIPA 208
[Close]
BLAST of SAM-dependent methyltransferase vs. Ensembl Nematostella
Match: EDO29865 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T2F0])

HSP 1 Score: 66.2402 bits (160), Expect = 1.040e-12
Identity = 48/161 (29.81%), Postives = 78/161 (48.45%), Query Frame = 2 
Query:  197 IMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLRHSYITTHQKEIIPEIERLNKENVIKIRMLSE-IPDYYPGVPA 676
            I+ S L  LF    +S  ILD GAGTGL+    ++LGY  ID+L+ +  M+  AK KN+Y       +   +    I +D YD +I  G  S  ++ +  +  ++  +K GG+   +L        + E    I+ L +E   K  + S+ + DY   +PA
Sbjct:  157 ILDSTLATLFPGNHQSLHILDAGAGTGLMGVSFKRLGYTIIDALDFSQEMLNCAKEKNVYRRLICAPIGEGKH-SDISQDNYDVLICTGTLSSPNVRASGLDELIHYVKPGGVICFTLHLHLYDNRESEYRKRIDELEREG--KWSLYSKHVTDYNKYIPA 314
[Close]
BLAST of SAM-dependent methyltransferase vs. Ensembl Medaka
Match: mettl27 (methyltransferase like 27 [Source:NCBI gene;Acc:101164667])

HSP 1 Score: 77.0258 bits (188), Expect = 1.265e-16
Identity = 45/178 (25.28%), Postives = 88/178 (49.44%), Query Frame = 2 
Query:  128 NLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLRHSYITTHQKEIIP-EIERLNKENVIKIRMLSEIPDY 658
            N YN W+K YD+ ++  +     +    +L  FS   KS  +LD+  GTGLVA++++K G+     ++ + AM+ +AK   LY +  ++ L   EP   +    +D ++  G  S   +    ++ + +  K GGL   + R +      K  +  E++++ KE +     ++E+ D+
Sbjct:   27 NFYNSWAKNYDQHVAVLDYRAPTLAADSVLPHFSGSRKSAALLDVACGTGLVAKQMKKHGFELFVGVDGSDAMLNEAKESGLYQD-LKQCLLGEEPFPALWAGFFDVVMIVGALSLGQVPVAVVRDLCEAAKPGGLICMTTRSNSDNLEYKTALERELQQMEKEGLWSCVEITEVKDW 203
[Close]
BLAST of SAM-dependent methyltransferase vs. Ensembl Medaka
Match: mettl27 (methyltransferase like 27 [Source:NCBI gene;Acc:101164667])

HSP 1 Score: 77.0258 bits (188), Expect = 1.265e-16
Identity = 45/178 (25.28%), Postives = 88/178 (49.44%), Query Frame = 2 
Query:  128 NLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLRHSYITTHQKEIIP-EIERLNKENVIKIRMLSEIPDY 658
            N YN W+K YD+ ++  +     +    +L  FS   KS  +LD+  GTGLVA++++K G+     ++ + AM+ +AK   LY +  ++ L   EP   +    +D ++  G  S   +    ++ + +  K GGL   + R +      K  +  E++++ KE +     ++E+ D+
Sbjct:   27 NFYNSWAKNYDQHVAVLDYRAPTLAADSVLPHFSGSRKSAALLDVACGTGLVAKQMKKHGFELFVGVDGSDAMLNEAKESGLYQD-LKQCLLGEEPFPALWAGFFDVVMIVGALSLGQVPVAVVRDLCEAAKPGGLICMTTRSNSDNLEYKTALERELQQMEKEGLWSCVEITEVKDW 203
[Close]
BLAST of SAM-dependent methyltransferase vs. Ensembl Medaka
Match: mettl27 (methyltransferase like 27 [Source:NCBI gene;Acc:101164667])

HSP 1 Score: 72.0182 bits (175), Expect = 8.821e-15
Identity = 53/203 (26.11%), Postives = 95/203 (46.80%), Query Frame = 2 
Query:   47 MSAS--ENDKGIVYIDHKIQNITNFIELENLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLRHSYITTHQKEIIP-EIERLNKENVIKIRMLSE 646
            MSA   EN K  +   H+    T   E  N YN W+K YD+ ++  +     +    +L  FS   KS  +LD+  GTGLVA++++K G+     ++ + AM+ +AK   LY +  +  L   EP   +    +D ++  G  S   +    ++ + +  K GGL   + R +      K  +  E++++ KE +     ++E
Sbjct:    1 MSADPFENVKATILSAHES---TTPREKVNFYNSWAKNYDQHVAVLDYRAPTLAADSVLPHFSGSRKSAALLDVACGTGLVAKQMKKHGFELFVGVDGSDAMLNEAKESGLYQDLKQCLL-GEEPFPALWAGFFDVVMIVGALSLGQVPVAVVRDLCEAAKPGGLICMTTRSNSDNLEYKTALERELQQMEKEGLWSCVEITE 199
[Close]
BLAST of SAM-dependent methyltransferase vs. Planmine SMEST
Match: SMESG000018166.1 (SMESG000018166.1)

HSP 1 Score: 328.561 bits (841), Expect = 1.557e-115
Identity = 160/165 (96.97%), Postives = 162/165 (98.18%), Query Frame = 2 
Query:   47 MSASENDKGIVYIDHKIQNITNFIELENLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFI 541
            MSASENDKGIVYID KIQNITNFIELENLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNID+LESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSG  +I
Sbjct:    1 MSASENDKGIVYIDQKIQNITNFIELENLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDALESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGRKYI 165
[Close]
BLAST of SAM-dependent methyltransferase vs. Planmine SMEST
Match: SMESG000018162.1 (SMESG000018162.1)

HSP 1 Score: 334.724 bits (857), Expect = 2.057e-115
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 2 
Query:   47 MSASENDKGIVYIDHKIQNITNFIELENLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSG 529
            MSASENDKGIVYIDHKIQNITNFIELENLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSG
Sbjct:    1 MSASENDKGIVYIDHKIQNITNFIELENLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSG 161
[Close]
BLAST of SAM-dependent methyltransferase vs. Planmine SMEST
Match: SMESG000018166.1 (SMESG000018166.1)

HSP 1 Score: 261.922 bits (668), Expect = 9.246e-90
Identity = 127/129 (98.45%), Postives = 128/129 (99.22%), Query Frame = 2 
Query:   47 MSASENDKGIVYIDHKIQNITNFIELENLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQE 433
            MSASENDKGIVYID KIQNITNFIELENLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNID+LESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQE
Sbjct:    1 MSASENDKGIVYIDQKIQNITNFIELENLYNEWSKTYDEDLSKPNSYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDALESNAAMIAKAKVKNLYLNYYEEFLEPNEPVKTIQE 129
[Close]
BLAST of SAM-dependent methyltransferase vs. Planmine SMEST
Match: SMESG000070491.1 (SMESG000070491.1)

HSP 1 Score: 150.214 bits (378), Expect = 1.047e-44
Identity = 82/193 (42.49%), Postives = 127/193 (65.80%), Query Frame = 2 
Query:  128 NLYNEWSKTYDEDLSKPN-SYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFL--EPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLRHSYITTHQKEIIPEIERLNKENVIKIRMLSEIPDYYPGVPAFGIVLEK 697
            +LY  W++TY++D+   +  Y GF ++SK++DLFS  +K  +ILDI AGTGLV++EL+KL Y NID+++S  AM+ KAKVKNLY NY+ +F   +PN+P    +  TYD +  AG FS  H++   I  ++K  K  GL     R+  I+++QKE+  EI RL  E +I++ + +EIP YY  V  + +V+++
Sbjct:   24 DLYTNWAETYEKDVVLTDEGYKGFEIISKIIDLFSV-NKDLKILDIAAGTGLVSQELKKLEYYNIDAMDSCEAMLTKAKVKNLYKNYFCQFAGEDPNDP----KYGTYDHVSIAGGFSKNHMNYTDILKLVKYSKINGLTSLVFRYEAISSYQKEVPVEIHRLKSEGIIELILETEIPKYYTNVSGYLVVMKR 211
[Close]
BLAST of SAM-dependent methyltransferase vs. Planmine SMEST
Match: SMESG000070472.1 (SMESG000070472.1)

HSP 1 Score: 142.51 bits (358), Expect = 2.923e-38
Identity = 80/187 (42.78%), Postives = 121/187 (64.71%), Query Frame = 2 
Query:  128 NLYNEWSKTYDEDLSKPN-SYCGFIMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAMIAKAKVKNLYLNYYEEFL--EPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRHIKPMLKLLKSGGLFITSLRHSYITTHQKEIIPEIERLNKENVIKIRMLSEIPDYYPGVPAF 679
            +LY  W++TY++D+   +  Y GF ++SK++DLFS  +K  +ILDI AGTGLV++EL+KL Y NID+++   AM+ KAKVKNLY NY+ +F   +PN+P    +  TYD +  AG FS  H++   I  ++K  K  GL   + R+  I+++QKE+  EI RL  E +I++ + +EIP YY     F
Sbjct:   25 DLYTNWAETYEKDVVLTDEGYKGFEIISKIIDLFSV-NKDLKILDIAAGTGLVSQELKKLEYYNIDAMDPCEAMLTKAKVKNLYKNYFCQFAGEDPNDP----KYGTYDHVSIAGGFSKNHMNYTDILKLVKYSKINGLTSLAFRYEAISSYQKEVPLEIHRLQNEGIIEVILETEIPKYYTNTYIF 206
The following BLAST results are available for this feature:
BLAST of SAM-dependent methyltransferase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
METTL273.584e-1226.29methyltransferase like 27 [Source:HGNC Symbol;Acc:... [more]
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BLAST of SAM-dependent methyltransferase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAM-dependent methyltransferase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAM-dependent methyltransferase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mettl271.754e-1123.93methyltransferase like 27 [Source:ZFIN;Acc:ZDB-GEN... [more]
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BLAST of SAM-dependent methyltransferase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
vegfb6.966e-1225.35vascular endothelial growth factor B [Source:Xenba... [more]
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BLAST of SAM-dependent methyltransferase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Mettl279.591e-1427.15methyltransferase like 27 [Source:MGI Symbol;Acc:M... [more]
Mettl271.210e-1326.97methyltransferase like 27 [Source:MGI Symbol;Acc:M... [more]
Mettl271.210e-1326.97methyltransferase like 27 [Source:MGI Symbol;Acc:M... [more]
Mettl271.210e-1326.97methyltransferase like 27 [Source:MGI Symbol;Acc:M... [more]
Mettl271.088e-828.26methyltransferase like 27 [Source:MGI Symbol;Acc:M... [more]
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BLAST of SAM-dependent methyltransferase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q8N6F8|MET27_HUMAN1.721e-1126.29Methyltransferase-like protein 27 OS=Homo sapiens ... [more]
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BLAST of SAM-dependent methyltransferase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A433U3L97.233e-3032.44Methyltransf_11 domain-containing protein OS=Elysi... [more]
C3ZBD15.182e-2939.61Methyltransf_25 domain-containing protein OS=Branc... [more]
A0A1I8IBM41.224e-2832.31MTS domain-containing protein OS=Macrostomum ligna... [more]
A0A267EL552.089e-2831.89Methyltransf_25 domain-containing protein OS=Macro... [more]
A0A267DED22.451e-2831.89Methyltransf_25 domain-containing protein OS=Macro... [more]
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BLAST of SAM-dependent methyltransferase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mettl272.538e-1124.39methyltransferase like 27 [Source:ZFIN;Acc:ZDB-GEN... [more]
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BLAST of SAM-dependent methyltransferase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAM-dependent methyltransferase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAM-dependent methyltransferase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 4
Match NameE-valueIdentityDescription
EDO355832.019e-1932.70Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO459054.239e-1928.83Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO421505.521e-1628.27Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO298651.040e-1229.81Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SAM-dependent methyltransferase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
mettl271.265e-1625.28methyltransferase like 27 [Source:NCBI gene;Acc:10... [more]
mettl271.265e-1625.28methyltransferase like 27 [Source:NCBI gene;Acc:10... [more]
mettl278.821e-1526.11methyltransferase like 27 [Source:NCBI gene;Acc:10... [more]
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BLAST of SAM-dependent methyltransferase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000018166.11.557e-11596.97SMESG000018166.1[more]
SMESG000018162.12.057e-115100.00SMESG000018162.1[more]
SMESG000018166.19.246e-9098.45SMESG000018166.1[more]
SMESG000070491.11.047e-4442.49SMESG000070491.1[more]
SMESG000070472.12.923e-3842.78SMESG000070472.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028146 ID=SMED30028146|Name=SAM-dependent methyltransferase|organism=Schmidtea mediterranea sexual|type=transcript|length=770bp
ATATGTATATATATATATATATACTTCACTATAATATCATAATCAAATGA
GTGCAAGCGAAAATGATAAAGGAATTGTATATATTGATCATAAAATTCAA
AATATTACAAATTTCATTGAATTAGAAAATCTTTACAACGAATGGTCAAA
AACATATGATGAAGATTTATCAAAACCGAATTCATATTGTGGTTTCATCA
TGGTCTCGAAATTGTTGGACTTATTTTCATTTCGAGATAAATCAAGAAGA
ATTTTAGATATTGGAGCAGGAACTGGCTTAGTTGCAGAAGAACTAAGAAA
ATTGGGATACAATAACATCGATTCTCTGGAATCAAATGCAGCAATGATAG
CAAAAGCAAAAGTAAAAAATTTATATTTAAATTACTATGAAGAGTTTCTC
GAACCTAATGAGCCTGTTAAAACAATTCAAGAAGATACTTACGACAGAAT
AATAGCAGCAGGGGTTTTTAGTCCTGCACATCTGGATTCTCGGCACATTA
AACCAATGTTGAAATTGTTGAAGTCAGGAGGACTTTTCATAACTAGTTTA
CGGCATTCATACATAACAACTCATCAAAAAGAGATTATTCCTGAAATTGA
GCGACTTAATAAAGAAAATGTTATCAAAATAAGGATGTTGAGTGAAATTC
CAGATTATTATCCAGGAGTCCCTGCTTTTGGAATTGTTTTAGAAAAAGTT
TAGATTCATGAACTGTAACTTTTATTGATTTAAAGATAAAAATATTTGAA
AGCTGTTTAACTACCTACAT
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protein sequence of SMED30028146-orf-1

>SMED30028146-orf-1 ID=SMED30028146-orf-1|Name=SMED30028146-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=219bp
MSASENDKGIVYIDHKIQNITNFIELENLYNEWSKTYDEDLSKPNSYCGF
IMVSKLLDLFSFRDKSRRILDIGAGTGLVAEELRKLGYNNIDSLESNAAM
IAKAKVKNLYLNYYEEFLEPNEPVKTIQEDTYDRIIAAGVFSPAHLDSRH
IKPMLKLLKSGGLFITSLRHSYITTHQKEIIPEIERLNKENVIKIRMLSE
IPDYYPGVPAFGIVLEKV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0016740transferase activity
GO:0008168methyltransferase activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR029063SAM-dependent_MTases
Vocabulary: biological process
TermDefinition
GO:0008152metabolic process
GO:0032259methylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF13489Methyltransf_23coord: 54..170
e-value: 1.4E-8
score: 34.7
NoneNo IPR availableGENE3DG3DSA:3.40.50.150coord: 18..198
e-value: 2.8E-17
score: 64.7
NoneNo IPR availablePANTHERPTHR43591:SF29METHYLTRANSFERASE-LIKE PROTEIN 27coord: 24..181
NoneNo IPR availablePANTHERPTHR43591FAMILY NOT NAMEDcoord: 24..181
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 68..170
e-value: 1.86238E-11
score: 57.0546
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILYSSF53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 28..188