SMED30028142

Overview
NameSMED30028142
Smed IDSMED30028142
Length (bp)685
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30028142 (SMED30028142) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30028142 (SMED30028142) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30028142 (SMED30028142) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30028142 (SMED30028142) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30028142

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30028142h1SMcG0010329 Contig55348newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028142h1SMcG0010329 Contig55348uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028142h1SMcG0018485 Contig55348newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30028142h1SMcG0018485 Contig55348uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30028142h1SMcG0018485 SMED30028142smed_20140614PMID:26457503
Tu et al., 2015
whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30028142h1SMcG0018485 SMED30028142smed_20140614PMID:26457503
Tu et al., 2015
whole organism asexual adult colorimetric in situ hybridization evidence
Category 3 cellSMED30028142h1SMcG0018485 dd_Smed_v6_379_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30028142 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002874:6065..6800 -3369
v31.002874:27606..28341 -3369
Homology
BLAST of SMED30028142 vs. Planmine SMEST
Match: SMESG000061765.1 (SMESG000061765.1)

HSP 1 Score: 267.7 bits (683), Expect = 1.445e-92
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 3
Query:  207 MKTSILIFCVLISAVFTDEEFQLSKHCIEVLTPLSKDCDRRYHVADTFCSQGYAKINKICTGKESVYCGLQYENHDKACKQVQNNFWNHCDDIYRNYRQQCASVKEIKEETLEIAADANANKKKYEVDI 593
            MKTSILIFCVLISAVFTDEEFQLSKHCIEVLTPLSKDCDRRYHVADTFCSQGYAKINKICTGKESVYCGLQYENHDKACKQVQNNFWNHCDDIYRNYRQQCASVKEIKEETLEIAADANANKKKYEVDI
Sbjct:    1 MKTSILIFCVLISAVFTDEEFQLSKHCIEVLTPLSKDCDRRYHVADTFCSQGYAKINKICTGKESVYCGLQYENHDKACKQVQNNFWNHCDDIYRNYRQQCASVKEIKEETLEIAADANANKKKYEVDI 129          
BLAST of SMED30028142 vs. Planmine SMEST
Match: SMESG000061762.1 (SMESG000061762.1)

HSP 1 Score: 267.7 bits (683), Expect = 1.445e-92
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 3
Query:  207 MKTSILIFCVLISAVFTDEEFQLSKHCIEVLTPLSKDCDRRYHVADTFCSQGYAKINKICTGKESVYCGLQYENHDKACKQVQNNFWNHCDDIYRNYRQQCASVKEIKEETLEIAADANANKKKYEVDI 593
            MKTSILIFCVLISAVFTDEEFQLSKHCIEVLTPLSKDCDRRYHVADTFCSQGYAKINKICTGKESVYCGLQYENHDKACKQVQNNFWNHCDDIYRNYRQQCASVKEIKEETLEIAADANANKKKYEVDI
Sbjct:    1 MKTSILIFCVLISAVFTDEEFQLSKHCIEVLTPLSKDCDRRYHVADTFCSQGYAKINKICTGKESVYCGLQYENHDKACKQVQNNFWNHCDDIYRNYRQQCASVKEIKEETLEIAADANANKKKYEVDI 129          
BLAST of SMED30028142 vs. Planmine SMEST
Match: SMESG000061767.1 (SMESG000061767.1)

HSP 1 Score: 123.635 bits (309), Expect = 1.018e-35
Identity = 59/127 (46.46%), Postives = 82/127 (64.57%), Query Frame = 3
Query:  207 MKTSILIFCVL-ISAVFTDEEFQLSKHCIEVLTPLSKDCDRRYHVADTFCSQGYAKINKICTGKESVYCGLQYENHDKACKQVQNNFWNHCDDIYRNYRQQCASVKEIKEETLEIAADANANKKKYE 584
            MK   +  C L ++ VF+ +    S  C ++LTP ++ CDR+Y  AD FCS GY  INK C GKE++ CG +++ HD  CK+ Q+ FW+ CD +Y  YR +CAS KEIKEE  EIA D+  +KK Y+
Sbjct:    1 MKNLSIFLCFLSVAVVFSTKWVPPSNRCSKILTPAARRCDRKYKEADNFCSAGYVAINKDCKGKETIDCGKRFDQHDADCKEKQDIFWDLCDSVYTAYRNECASPKEIKEELQEIADDSKESKKHYK 127          
BLAST of SMED30028142 vs. Planmine SMEST
Match: SMESG000022139.1 (SMESG000022139.1)

HSP 1 Score: 116.701 bits (291), Expect = 3.458e-33
Identity = 54/123 (43.90%), Postives = 76/123 (61.79%), Query Frame = 3
Query:  204 IMKTSILIFCVLISAVFTDEEFQLSKHCIEVLTPLSKDCDRRYHVADTFCSQGYAKINKICTGKESVYCGLQYENHDKACKQVQNNFWNHCDDIYRNYRQQCASVKEIKEETLEIAADANANK 572
            + K SI+ F + ++    D     S  C  VL P SK CD+ Y   D FC+ G+AK+NK+C GK++V CG  Y+     CK+ Q+NFW+ CD +Y  YR++CA+ +EIKEE  EIA +A  NK
Sbjct:    1 MKKFSIIAFSIFVTLAVADVWIPPSARCKNVLVPASKQCDKNYKETDEFCNGGFAKLNKVCYGKDTVECGDAYKAFTTLCKEKQDNFWDECDPVYTAYRKECATPEEIKEENQEIAEEAKDNK 123          
BLAST of SMED30028142 vs. Planmine SMEST
Match: SMESG000028420.1 (SMESG000028420.1)

HSP 1 Score: 94.7449 bits (234), Expect = 1.004e-24
Identity = 45/115 (39.13%), Postives = 66/115 (57.39%), Query Frame = 3
Query:  204 IMKTSILIFCVLISAVFTDEEFQLSKHCIEVLTPLSKDCDRRYHVADTFCSQGYAKINKICTGKESVYCGLQYENHDKACKQVQNNFWNHCDDIYRNYRQQCASVKEIKEETLEI 548
            + K S+     ++  V + +     + C+++LTP +K CD RY   D FCS GY  I+K C G +SV CG+QY  H   C + Q+ FW  C  +Y+ Y  +CAS +E KEE+ EI
Sbjct:    1 MNKISVYALFAVVVIVNSTKWVPSPEKCLKILTPAAKGCDSRYTETDLFCSVGYKHIDKSCKGHDSVECGVQYAKHKDDCTEKQDYFWKVCGPVYKTYSDECASNEERKEESAEI 115          
The following BLAST results are available for this feature:
BLAST of SMED30028142 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028142 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028142 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028142 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028142 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028142 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028142 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028142 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028142 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028142 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028142 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028142 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028142 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028142 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000061765.11.445e-92100.00SMESG000061765.1[more]
SMESG000061762.11.445e-92100.00SMESG000061762.1[more]
SMESG000061767.11.018e-3546.46SMESG000061767.1[more]
SMESG000022139.13.458e-3343.90SMESG000022139.1[more]
SMESG000028420.11.004e-2439.13SMESG000028420.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028142 ID=SMED30028142|Name=SMED30028142|organism=Schmidtea mediterranea sexual|type=transcript|length=685bp
GATGAAATAAATTATTACTGTGATATTACATATTAACAAATTTTTTATTA
TGTATTTCAATATTTTTTTCAAGAAAAGTACTATAACGTATTCGAGTAAA
TGTTAGGCGTAGTAAGAAGACCTACGTAAAACTACACGAATAAAAAGCCA
ACTGATATATTATTTATATAAATTCTTCTGAAATCTATTTCTAATTTAGA
ACAATAATGAAAACTTCAATTCTTATCTTTTGTGTTTTAATTTCTGCAGT
GTTTACGGATGAGGAGTTTCAACTATCAAAACACTGTATAGAGGTCTTGA
CACCGCTTTCAAAAGACTGCGACAGAAGATATCATGTTGCCGATACTTTC
TGCAGTCAAGGATATGCGAAAATTAACAAAATTTGCACCGGTAAAGAGAG
CGTTTATTGCGGTCTACAATATGAAAACCATGATAAAGCTTGTAAACAAG
TGCAAAACAATTTCTGGAATCATTGTGACGACATTTACCGAAATTATCGC
CAACAATGTGCTTCAGTTAAGGAAATCAAAGAAGAAACGTTAGAAATCGC
AGCCGATGCCAATGCGAATAAGAAAAAGTACGAAGTCGACATTTAAGTTT
CTAATGATATGTAATAAAATGAATAAATTTCATTTCAATTTTTTTATATG
TAAAATTTTAAATACAGAAATAAATAAAATCAGAC
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protein sequence of SMED30028142-orf-1

>SMED30028142-orf-1 ID=SMED30028142-orf-1|Name=SMED30028142-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=130bp
MKTSILIFCVLISAVFTDEEFQLSKHCIEVLTPLSKDCDRRYHVADTFCS
QGYAKINKICTGKESVYCGLQYENHDKACKQVQNNFWNHCDDIYRNYRQQ
CASVKEIKEETLEIAADANANKKKYEVDI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000035Category 3 cell
PLANA:0002032epidermal cell
PLANA:0002142parenchyma
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..17
score: 0.737