SMED30028103

Overview
NameSMED30028103
Smed IDSMED30028103
Length (bp)1545
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30028103 (SMED30028103) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30028103 (SMED30028103) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30028103 (SMED30028103) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30028103 (SMED30028103) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30028103

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30028103h1SMcG0021868 dd_Smed_v4_11827_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30028103h1SMcG0021868 dd_Smed_v4_11827_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx musculatureSMED30028103h1SMcG0021868 dd_Smed_v4_11827_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
enteric muscle cellSMED30028103h1SMcG0021868 dd_Smed_v4_11827_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30028103 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.008487:20386..21930 +7653
Homology
[Close]
BLAST of SMED30028103 vs. TrEMBL
Match: A0A0R3TSH8 (Uncharacterized protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS10594 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 9.224e-11
Identity = 89/347 (25.65%), Postives = 149/347 (42.94%), Query Frame = 3 
Query:  189 LGIILNGFSLHVLWKCMTTGQLGIKLVLLSDLVVCLAYVXXXXXXXXXXXXXXDFFRIRQLVAINRLSIHIS----------LFVSNWWIIALLVLRILTI---------------FSPKNINSWNKMLYHKLVYGLCWISAIAIIVPLRFHEQYT-RLIICVVPVALVSLEAGLIIYGIRRV---KPPKLTPNI---------KTLKSSYQEV-----PCSSSTSSTYIKVNPVNVQPSSKCM---------KPYNNIVLTILTLALNYLILDCLQFID----------------------ALHTVCAISKDPLPTWFMLLI-NIFSLLKRIFNFVILCAKCRYFR 1004
            +GIILN F+  +L +  T G L IKL+L+SD +VCL Y+++    +I     S+     Q  +   +  HIS            VSNW+++ ++V R ++I               F  K  N W       + +G CWI  +     L ++ Q T  + + V+P+ LV + +  +++ +R++     P  TP++         K+ K    E      P + S  +       +   PS++           K Y+ I +TIL+LA++++ILD +Q ID                       L       K  L    M ++ N  +L K + NF+ILCA  R+FR
Sbjct:   41 IGIILNFFAFGILRRKATMGVLLIKLILISDGLVCLFYLLNQTWTDIWMKYFSNSMVSFQY-SKKSIPTHISNILRKLAITATGVSNWYVVIMIVHRCVSIYMNPLRSRIDRSLWAFVQKPTNLW-------IAFGACWIFGLIQAFLLPYNNQITLDVFMYVLPLILVFIFSVSLLFKLRKIHTNTDPANTPSMCCFSLHGVNKSHKGDSDETFTQIRPATLSAENRLRSSEEIQTNPSTRSKRMPAEMDRKKTYHRITMTILSLAISFVILDSMQLIDLLIRIPWDALLGGIERVPANETGLDVYVTEVKSKLKQHVMSIVKNTCTLGKTLANFIILCAHSRHFR 379
[Close]
BLAST of SMED30028103 vs. Planmine SMEST
Match: SMESG000081188.1 (SMESG000081188.1)

HSP 1 Score: 797.734 bits (2059), Expect = 0.000e+0
Identity = 408/410 (99.51%), Postives = 408/410 (99.51%), Query Frame = 3 
Query:  135 DDKVIMTMINLSVLMLGTLGIILNGFSLHVLWKCMTTGQLGIKLVLLSDLVVCLAYVXXXXXXXXXXXXXXDFFRIRQLVAINRLSIHISLFVSNWWIIALLVLRILTIFSPKNINSWNKMLYHKLVYGLCWISAIAIIVPLRFHEQYTRLIICVVPVALVSLEAGLIIYGIRRVKPPKLTPNIKTLKSSYQEVPCSSSTSSTYIKVNPVNVQPSSKCMKPYNNIVLTILTLALNYLILDCLQFIDALHTVCAISKDPLPTWFMLLINIFSLLKRIFNFVILCAKCRYFRKIMNLKINLISRHFHRHQNLKKRNLSNHALQKLNDPWKGYSHCTDCQKPVLFVKENELLSESNLIGRLLETAPFPMKGLSNKNDLKKDHCINSYYSAFEVLPFMSQISHQSNNQVYSTKY 1364
            DDKVIMTMINLSVLMLGTLGIILNGFSLHVLWKCMTTGQLGIKLVLLSDLVVCLAYVISNIQNNIANSITSDFFRIRQLVAINRLSIHISLFVSNWWIIALLVLRILTIFSPKNINSWNKMLYHKLVYGLCWISAIAIIVPLRFHEQYTRLIICVVPVALVSLEAGLIIYGIRRVKPPKLTPNIKTLKSSYQEVPCSSSTSSTYIKVNP NVQPSSKCMKPYNNIVLTILTLALNYLILDCLQFIDALHTVCAISKDPLPTWFMLLINIFSLLKRIFNFVILCAKCRYFRKIMNLKINLISRHFHRHQNLKKRNLSNHALQ LNDPWKGYSHCTDCQKPVLFVKENELLSESNLIGRLLETAPFPMKGLSNKNDLKKDHCINSYYSAFEVLPFMSQISHQSNNQVYSTKY
Sbjct:   11 DDKVIMTMINLSVLMLGTLGIILNGFSLHVLWKCMTTGQLGIKLVLLSDLVVCLAYVISNIQNNIANSITSDFFRIRQLVAINRLSIHISLFVSNWWIIALLVLRILTIFSPKNINSWNKMLYHKLVYGLCWISAIAIIVPLRFHEQYTRLIICVVPVALVSLEAGLIIYGIRRVKPPKLTPNIKTLKSSYQEVPCSSSTSSTYIKVNPANVQPSSKCMKPYNNIVLTILTLALNYLILDCLQFIDALHTVCAISKDPLPTWFMLLINIFSLLKRIFNFVILCAKCRYFRKIMNLKINLISRHFHRHQNLKKRNLSNHALQNLNDPWKGYSHCTDCQKPVLFVKENELLSESNLIGRLLETAPFPMKGLSNKNDLKKDHCINSYYSAFEVLPFMSQISHQSNNQVYSTKY 420
[Close]
BLAST of SMED30028103 vs. Planmine SMEST
Match: SMESG000081188.1 (SMESG000081188.1)

HSP 1 Score: 704.901 bits (1818), Expect = 0.000e+0
Identity = 365/370 (98.65%), Postives = 367/370 (99.19%), Query Frame = 3 
Query:  135 DDKVIMTMINLSVLMLGTLGIILNGFSLHVLWKCMTTGQLGIKLVLLSDLVVCLAYVXXXXXXXXXXXXXXDFFRIRQLVAINRLSIHISLFVSNWWIIALLVLRILTIFSPKNINSWNKMLYHKLVYGLCWISAIAIIVPLRFHEQYTRLIICVVPVALVSLEAGLIIYGIRRVKPPKLTPNIKTLKSSYQEVPCSSSTSSTYIKVNPVNVQPSSKCMKPYNNIVLTILTLALNYLILDCLQFIDALHTVCAISKDPLPTWFMLLINIFSLLKRIFNFVILCAKCRYFRKIMNLKINLISRHFHRHQNLKKRNLSNHALQKLNDPWKGYSHCTDCQKPVLFVKENELLSESNLIGRLLETAPFPMKGLS 1244
            DDKVIMTMINLSVLMLGTLGIILNGFSLHVLWKCMTTGQLGIKLVLLSDLVVCLAYVISNIQNNIANSITSDFFRIRQLVAINRLSIHISLFVSNWWIIALLVLRILTIFSPKNINSWNKMLYHKLVYGLCWISAIAIIVPLRFHEQYTRLIICVVPVALVSLEAGLIIYGIRRVKPPKLTPNIKTLKSSYQEVPCSSSTSSTYIKVNP NVQPSSKCMKPYNNIVLTILTLALNYLILDCLQFIDALHTVCAISKDPLPTWFMLLINIFSLLKRIFNFVILCAKCRYFRKIMNLKINLISRHFHRHQNLKKRNLSNHALQ LNDPWKGYSHCTDCQKPVLFVKENELLSESNLIGRLLETAPFPMK ++
Sbjct:   11 DDKVIMTMINLSVLMLGTLGIILNGFSLHVLWKCMTTGQLGIKLVLLSDLVVCLAYVISNIQNNIANSITSDFFRIRQLVAINRLSIHISLFVSNWWIIALLVLRILTIFSPKNINSWNKMLYHKLVYGLCWISAIAIIVPLRFHEQYTRLIICVVPVALVSLEAGLIIYGIRRVKPPKLTPNIKTLKSSYQEVPCSSSTSSTYIKVNPANVQPSSKCMKPYNNIVLTILTLALNYLILDCLQFIDALHTVCAISKDPLPTWFMLLINIFSLLKRIFNFVILCAKCRYFRKIMNLKINLISRHFHRHQNLKKRNLSNHALQNLNDPWKGYSHCTDCQKPVLFVKENELLSESNLIGRLLETAPFPMKVIT 380

HSP 2 Score: 57.3806 bits (137), Expect = 1.317e-8
Identity = 26/26 (100.00%), Postives = 26/26 (100.00%), Query Frame = 1 
Query: 1294 PLKFYLLCHKYLISQTIKSIRPNTEP 1371
            PLKFYLLCHKYLISQTIKSIRPNTEP
Sbjct:  382 PLKFYLLCHKYLISQTIKSIRPNTEP 407
[Close]
BLAST of SMED30028103 vs. Planmine SMEST
Match: SMESG000081188.1 (SMESG000081188.1)

HSP 1 Score: 592.038 bits (1525), Expect = 0.000e+0
Identity = 288/290 (99.31%), Postives = 288/290 (99.31%), Query Frame = 3 
Query:  495 MLYHKLVYGLCWISAIAIIVPLRFHEQYTRLIICVVPVALVSLEAGLIIYGIRRVKPPKLTPNIKTLKSSYQEVPCSSSTSSTYIKVNPVNVQPSSKCMKPYNNIVLTILTLALNYLILDCLQFIDALHTVCAISKDPLPTWFMLLINIFSLLKRIFNFVILCAKCRYFRKIMNLKINLISRHFHRHQNLKKRNLSNHALQKLNDPWKGYSHCTDCQKPVLFVKENELLSESNLIGRLLETAPFPMKGLSNKNDLKKDHCINSYYSAFEVLPFMSQISHQSNNQVYSTKY 1364
            MLYHKLVYGLCWISAIAIIVPLRFHEQYTRLIICVVPVALVSLEAGLIIYGIRRVKPPKLTPNIKTLKSSYQEVPCSSSTSSTYIKVNP NVQPSSKCMKPYNNIVLTILTLALNYLILDCLQFIDALHTVCAISKDPLPTWFMLLINIFSLLKRIFNFVILCAKCRYFRKIMNLKINLISRHFHRHQNLKKRNLSNHALQ LNDPWKGYSHCTDCQKPVLFVKENELLSESNLIGRLLETAPFPMKGLSNKNDLKKDHCINSYYSAFEVLPFMSQISHQSNNQVYSTKY
Sbjct:    1 MLYHKLVYGLCWISAIAIIVPLRFHEQYTRLIICVVPVALVSLEAGLIIYGIRRVKPPKLTPNIKTLKSSYQEVPCSSSTSSTYIKVNPANVQPSSKCMKPYNNIVLTILTLALNYLILDCLQFIDALHTVCAISKDPLPTWFMLLINIFSLLKRIFNFVILCAKCRYFRKIMNLKINLISRHFHRHQNLKKRNLSNHALQNLNDPWKGYSHCTDCQKPVLFVKENELLSESNLIGRLLETAPFPMKGLSNKNDLKKDHCINSYYSAFEVLPFMSQISHQSNNQVYSTKY 290
The following BLAST results are available for this feature:
BLAST of SMED30028103 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028103 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028103 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028103 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028103 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028103 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028103 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028103 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
A0A0R3TSH89.224e-1125.65Uncharacterized protein OS=Rodentolepis nana OX=10... [more]
back to top
BLAST of SMED30028103 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028103 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028103 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028103 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028103 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30028103 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000081188.10.000e+099.51SMESG000081188.1[more]
SMESG000081188.11.317e-898.65SMESG000081188.1[more]
SMESG000081188.10.000e+099.31SMESG000081188.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028103 ID=SMED30028103|Name=SMED30028103|organism=Schmidtea mediterranea sexual|type=transcript|length=1545bp
GCCAATCAGATTTCAGTAAGTTGTTAGATAAATTCATTGTATTTTTAATA
ATTCTTCAAATCTATCAGTAATAATTTCAGAAAGGTAATTGTTGGTAAAA
AATTATGTCAAACTATTCAAGTAATTCGTCATCGGACGATAAAGTTATTA
TGACCATGATTAATTTATCAGTATTAATGCTTGGAACTTTGGGCATCATC
TTAAATGGTTTCTCTCTTCACGTTCTATGGAAATGCATGACAACGGGACA
ACTGGGAATCAAACTAGTTTTACTGAGTGATTTAGTAGTTTGCCTGGCTT
ATGTAATATCAAATATTCAGAACAATATTGCTAACAGTATTACATCTGAC
TTTTTCCGAATCAGACAATTAGTTGCCATCAATCGGTTATCAATTCACAT
ATCTTTATTTGTAAGTAATTGGTGGATAATAGCACTATTGGTTTTACGAA
TTCTCACGATATTTTCGCCGAAAAATATTAATTCTTGGAACAAGATGTTG
TATCACAAATTGGTTTATGGATTATGCTGGATTTCCGCTATTGCTATCAT
TGTTCCACTGAGATTTCATGAACAGTATACACGTTTAATAATTTGTGTTG
TTCCAGTTGCATTGGTTTCTTTGGAAGCGGGTTTAATTATTTACGGAATC
AGACGAGTTAAACCACCAAAATTAACACCAAACATCAAAACACTGAAAAG
TAGCTATCAAGAGGTTCCATGTTCGAGCTCCACTAGTAGTACTTATATAA
AAGTCAATCCAGTTAATGTTCAACCTTCTTCAAAATGTATGAAGCCTTAC
AACAATATTGTGTTGACAATTCTGACATTGGCGCTGAATTATTTAATTCT
TGATTGTTTACAATTTATCGATGCCCTTCATACTGTCTGTGCAATCAGTA
AAGATCCTCTACCTACCTGGTTCATGCTTTTAATTAATATTTTCTCATTA
CTGAAACGAATTTTCAATTTTGTCATCCTGTGTGCCAAATGTCGATATTT
TCGTAAAATAATGAATTTAAAAATCAATTTAATCTCTAGGCATTTTCACA
GACATCAGAATTTAAAAAAACGCAATTTATCAAATCACGCTCTTCAGAAA
CTAAATGATCCTTGGAAAGGTTATAGTCATTGTACAGACTGTCAGAAGCC
GGTTTTATTTGTGAAAGAAAATGAGTTATTATCAGAGTCGAATTTGATTG
GTCGATTACTCGAAACCGCTCCGTTTCCCATGAAAGGTTTGTCAAATAAA
AACGATTTGAAAAAAGACCATTGTATTAATAGTTATTACAGTGCCTTTGA
AGTTTTACCTTTTATGTCACAAATATCTCATCAGTCAAACAATCAAGTCT
ATTCGACCAAATACTGAGCCATGATTTTCCATATATATAATTATTCGATA
TTTTTCATATCAGAGCTGTGATATGTGTAAATATTTTCATTTATTTTGTG
TATTTTTAATATTTGTTTTTATTGTTGATCTATTGGAATTAAATGTCAAA
AAGTTTTTATCGAATGTTCTCAACTAAAAAATTTTCAAAGTGAAA
back to top

protein sequence of SMED30028103-orf-1

>SMED30028103-orf-1 ID=SMED30028103-orf-1|Name=SMED30028103-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=421bp
MSNYSSNSSSDDKVIMTMINLSVLMLGTLGIILNGFSLHVLWKCMTTGQL
GIKLVLLSDLVVCLAYVISNIQNNIANSITSDFFRIRQLVAINRLSIHIS
LFVSNWWIIALLVLRILTIFSPKNINSWNKMLYHKLVYGLCWISAIAIIV
PLRFHEQYTRLIICVVPVALVSLEAGLIIYGIRRVKPPKLTPNIKTLKSS
YQEVPCSSSTSSTYIKVNPVNVQPSSKCMKPYNNIVLTILTLALNYLILD
CLQFIDALHTVCAISKDPLPTWFMLLINIFSLLKRIFNFVILCAKCRYFR
KIMNLKINLISRHFHRHQNLKKRNLSNHALQKLNDPWKGYSHCTDCQKPV
LFVKENELLSESNLIGRLLETAPFPMKGLSNKNDLKKDHCINSYYSAFEV
LPFMSQISHQSNNQVYSTKY*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000478pharynx musculature
PLANA:0003104enteric muscle cell
PLANA:0003116parenchymal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 7..308
NoneNo IPR availableTMHMMTMhelixcoord: 272..294
NoneNo IPR availableTMHMMTMhelixcoord: 15..37
NoneNo IPR availableTMHMMTMhelixcoord: 132..154
NoneNo IPR availableTMHMMTMhelixcoord: 235..257
NoneNo IPR availableTMHMMTMhelixcoord: 50..72
NoneNo IPR availableTMHMMTMhelixcoord: 159..181
NoneNo IPR availableTMHMMTMhelixcoord: 97..119