Metallophos domain-containing protein

Overview
NameMetallophos domain-containing protein
Smed IDSMED30028096
Length (bp)1165
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Metallophos domain-containing protein (SMED30028096) t-SNE clustered cells

Violin plots show distribution of expression levels for Metallophos domain-containing protein (SMED30028096) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Metallophos domain-containing protein (SMED30028096) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Metallophos domain-containing protein (SMED30028096) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30028096

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
neoblastSMED30028096h1SMcG0016751 dd_Smed_v4_12322_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Metallophos domain-containing protein vs. Ensembl Celegans
Match: ZK792.7 (pep chromosome:WBcel235:IV:11694359:11696464:1 gene:WBGene00014079.1 transcript:ZK792.7a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZK792.7)

HSP 1 Score: 70.4774 bits (171), Expect = 5.576e-22
Identity = 37/86 (43.02%), Postives = 53/86 (61.63%), Query Frame = 1 
Query:  127 RILLVGDPQLIGFNYEYKLFGPINRWDADRFLKSGFRKAVQYTNPDVVVFLGDLFDEGEIATESQFVNYFHRFNDIFLSGLGEKPV 384
            RIL+V DPQLIG+  E   FG I+RWD+DR+L +G+  A     P  V+FLGDLFDEG  + + ++   + RF  I+    G+  +
Sbjct:   49 RILIVADPQLIGYKNE--KFGEISRWDSDRYLATGYSYAKWRFLPTTVMFLGDLFDEGIESNDDEWYETYERFIGIYPIDRGDNAI 132

HSP 2 Score: 52.373 bits (124), Expect = 5.576e-22
Identity = 61/212 (28.77%), Postives = 88/212 (41.51%), Query Frame = 3 
Query:  402 TIIIPGDNDIGGEYP----SVRNQ------NIVRRFMKRFNVSY------PVVKKIGNINFIGGDITSNEDFQKTLEFLIDKYKSTDFHVLFNHDSVHMDQKYFKYKPDIPLVITAHSHVSQYKYCEDCSEKYKKWSPTYKLESYEEVVFDLSPGLSK-------ILEITVPTCSYRMGVPYPGYGFLILSDKG------MLSYKVLWLPYR 950
             I I GDNDIGGE      S RNQ      N V     R+++S       P++K I         I         + +L++ YK +D           + QK       + L+++AH H +          +Y++ +P   L +    V D+SP   K       ++E+  PTCSYRMGV   GYG L +           + Y VLWLP R
Sbjct:  131 AIYIAGDNDIGGESELISESRRNQFYNYFRNNVSDLKDRYSISETYLFENPILKHITKAQVPVAKIMLTH-----VPYLVEGYKHSD-----------VGQK-------MDLILSAHDHTTGIY-------EYQRSAPRAVLFT---RVSDVSPTYFKNIGQNEPLIELQTPTCSYRMGVYSMGYGALSICRLNDDYRSVQVQYSVLWLPSR 309
[Close]
BLAST of Metallophos domain-containing protein vs. Ensembl Fly
Match: Mppe (gene:FBgn0259985 transcript:FBtr0088024)

HSP 1 Score: 88.1965 bits (217), Expect = 5.310e-20
Identity = 39/104 (37.50%), Postives = 64/104 (61.54%), Query Frame = 1 
Query:   46 NEYLLFEYCYFNWSLNYKLKRNIDNEFRILLVGDPQLIGFNYEYKLFGPINRWDADRFLKSGFRKAVQYTNPDVVVFLGDLFDEGEIATESQFVNYFHRFNDIF 357
            NE++++     +W     +   +DN  R+LL+ DPQ++G +Y+     P+ R+D+DR+L   F +A+ +T P ++VFLGDL DEG IAT  ++  Y  RF  I+
Sbjct:   26 NEFIVYYMAQSSWQ---PIDCKLDNCTRLLLIADPQILGNSYDRSSHSPLARYDSDRYLAKTFERALAFTQPHIIVFLGDLLDEGNIATAQEYKQYVQRFRRIY 126

HSP 2 Score: 28.8758 bits (63), Expect = 5.310e-20
Identity = 17/30 (56.67%), Postives = 18/30 (60.00%), Query Frame = 3 
Query:  411 IPGDNDIGGE---YPSVRNQNIVRRFMKRF 491
            +PGDNDIGGE   Y S  NQ   RRF   F
Sbjct:  137 VPGDNDIGGENGDYISNSNQ---RRFENEF 163
[Close]
BLAST of Metallophos domain-containing protein vs. Ensembl Fly
Match: Mppe (gene:FBgn0259985 transcript:FBtr0339583)

HSP 1 Score: 88.1965 bits (217), Expect = 5.666e-20
Identity = 39/104 (37.50%), Postives = 64/104 (61.54%), Query Frame = 1 
Query:   46 NEYLLFEYCYFNWSLNYKLKRNIDNEFRILLVGDPQLIGFNYEYKLFGPINRWDADRFLKSGFRKAVQYTNPDVVVFLGDLFDEGEIATESQFVNYFHRFNDIF 357
            NE++++     +W     +   +DN  R+LL+ DPQ++G +Y+     P+ R+D+DR+L   F +A+ +T P ++VFLGDL DEG IAT  ++  Y  RF  I+
Sbjct:   28 NEFIVYYMAQSSWQ---PIDCKLDNCTRLLLIADPQILGNSYDRSSHSPLARYDSDRYLAKTFERALAFTQPHIIVFLGDLLDEGNIATAQEYKQYVQRFRRIY 128

HSP 2 Score: 28.4906 bits (62), Expect = 5.666e-20
Identity = 17/30 (56.67%), Postives = 18/30 (60.00%), Query Frame = 3 
Query:  411 IPGDNDIGGE---YPSVRNQNIVRRFMKRF 491
            +PGDNDIGGE   Y S  NQ   RRF   F
Sbjct:  148 VPGDNDIGGENGDYISNSNQ---RRFENEF 174
[Close]
BLAST of Metallophos domain-containing protein vs. Ensembl Fly
Match: Mppe (gene:FBgn0259985 transcript:FBtr0088025)

HSP 1 Score: 88.1965 bits (217), Expect = 5.763e-20
Identity = 39/104 (37.50%), Postives = 64/104 (61.54%), Query Frame = 1 
Query:   46 NEYLLFEYCYFNWSLNYKLKRNIDNEFRILLVGDPQLIGFNYEYKLFGPINRWDADRFLKSGFRKAVQYTNPDVVVFLGDLFDEGEIATESQFVNYFHRFNDIF 357
            NE++++     +W     +   +DN  R+LL+ DPQ++G +Y+     P+ R+D+DR+L   F +A+ +T P ++VFLGDL DEG IAT  ++  Y  RF  I+
Sbjct:   28 NEFIVYYMAQSSWQ---PIDCKLDNCTRLLLIADPQILGNSYDRSSHSPLARYDSDRYLAKTFERALAFTQPHIIVFLGDLLDEGNIATAQEYKQYVQRFRRIY 128

HSP 2 Score: 28.4906 bits (62), Expect = 5.763e-20
Identity = 17/30 (56.67%), Postives = 18/30 (60.00%), Query Frame = 3 
Query:  411 IPGDNDIGGE---YPSVRNQNIVRRFMKRF 491
            +PGDNDIGGE   Y S  NQ   RRF   F
Sbjct:  139 VPGDNDIGGENGDYISNSNQ---RRFENEF 165
[Close]
BLAST of Metallophos domain-containing protein vs. Ensembl Fly
Match: Mppe (gene:FBgn0259985 transcript:FBtr0339582)

HSP 1 Score: 87.8113 bits (216), Expect = 6.254e-20
Identity = 39/104 (37.50%), Postives = 64/104 (61.54%), Query Frame = 1 
Query:   46 NEYLLFEYCYFNWSLNYKLKRNIDNEFRILLVGDPQLIGFNYEYKLFGPINRWDADRFLKSGFRKAVQYTNPDVVVFLGDLFDEGEIATESQFVNYFHRFNDIF 357
            NE++++     +W     +   +DN  R+LL+ DPQ++G +Y+     P+ R+D+DR+L   F +A+ +T P ++VFLGDL DEG IAT  ++  Y  RF  I+
Sbjct:   26 NEFIVYYMAQSSWQ---PIDCKLDNCTRLLLIADPQILGNSYDRSSHSPLARYDSDRYLAKTFERALAFTQPHIIVFLGDLLDEGNIATAQEYKQYVQRFRRIY 126

HSP 2 Score: 28.4906 bits (62), Expect = 6.254e-20
Identity = 17/30 (56.67%), Postives = 18/30 (60.00%), Query Frame = 3 
Query:  411 IPGDNDIGGE---YPSVRNQNIVRRFMKRF 491
            +PGDNDIGGE   Y S  NQ   RRF   F
Sbjct:  146 VPGDNDIGGENGDYISNSNQ---RRFENEF 172
[Close]
BLAST of Metallophos domain-containing protein vs. Ensembl Fly
Match: PGAP5 (gene:FBgn0031997 transcript:FBtr0079587)

HSP 1 Score: 56.9954 bits (136), Expect = 1.120e-8
Identity = 30/87 (34.48%), Postives = 47/87 (54.02%), Query Frame = 1 
Query:   97 KLKRNIDNEFRILLVGDPQLIGFNYEYKLFGPINRWDADRFLKSGFRKAVQYTNPDVVVFLGDLFDEGEIATESQFVNYFHRFNDIF 357
            K K+ +D+  R +++ DP L+G +  + L      W   R     F+ A +   PDVV  LGDLFDEG++ ++ QF  Y  R+  +F
Sbjct:   36 KRKKYVDDPLRAMILADPHLLGPHRGHWLDKLYREWHMTR----AFQAASRLFQPDVVFVLGDLFDEGDMVSDKQFQEYVWRYLKMF 118
[Close]
BLAST of Metallophos domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9VLR9|MPPE1_DROME (Metallophosphoesterase 1 homolog OS=Drosophila melanogaster OX=7227 GN=PGAP5 PE=2 SV=4)

HSP 1 Score: 56.9954 bits (136), Expect = 1.175e-7
Identity = 30/87 (34.48%), Postives = 47/87 (54.02%), Query Frame = 1 
Query:   97 KLKRNIDNEFRILLVGDPQLIGFNYEYKLFGPINRWDADRFLKSGFRKAVQYTNPDVVVFLGDLFDEGEIATESQFVNYFHRFNDIF 357
            K K+ +D+  R +++ DP L+G +  + L      W   R     F+ A +   PDVV  LGDLFDEG++ ++ QF  Y  R+  +F
Sbjct:   36 KRKKYVDDPLRAMILADPHLLGPHRGHWLDKLYREWHMTR----AFQAASRLFQPDVVFVLGDLFDEGDMVSDKQFQEYVWRYLKMF 118
[Close]
BLAST of Metallophos domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9UUH0|YKIC_SCHPO (Uncharacterized protein C630.12 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC630.12 PE=3 SV=1)

HSP 1 Score: 52.7582 bits (125), Expect = 3.119e-6
Identity = 32/81 (39.51%), Postives = 44/81 (54.32%), Query Frame = 1 
Query:  118 NEFRILLVGDPQLI---GFNYEYKLFGPINRWDADRFLKSGFRKAVQYTNPDVVVFLGDLFDEG-EIATESQFVNYFHRFN 348
            N  RI LV DPQL+    ++Y   L G I +W +D+FL+  +R   +   PD+   +GDL D G E ATE    +YF   N
Sbjct:   44 NPVRIALVADPQLVDDLTYDYPRPLIG-IVKWISDQFLRRHWRYLHKSLKPDITFIMGDLMDTGREFATEEFKKDYFRMMN 123
[Close]
BLAST of Metallophos domain-containing protein vs. TrEMBL
Match: A0A0B7AH84 (Metallophos domain-containing protein OS=Arion vulgaris OX=1028688 GN=ORF118330 PE=4 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 2.463e-41
Identity = 78/201 (38.81%), Postives = 106/201 (52.74%), Query Frame = 3 
Query:  402 TIIIPGDNDIGGEYPSVRNQNIVRRFMKRFNVSYPVVKKIGNINFIGGDITSNEDF----QKTLEFLIDKYKSTDFHVLFNHDSVHMDQKYFKY------KPDIPLVITAHSHVSQYKYCEDC---SEKYKKWSPTY-----KLESYEEVVFDLSPGLSKILEITVPTCSYRMGVPYPGYGFLILSDKGMLSYKVLWLPYR 950
            TI+IPGDND+GGE   VR    V RF K F     V+   G I+F+  DI    D     QK +  +  + KST F ++ NH+S+    K   Y      +P+I  ++T H H+SQ   C+ C    E    W P Y     K++ Y ++  DL+  L  + EI VPTCSYRMGVP  GYG  +++  G + Y VLWLP R
Sbjct:   87 TIVIPGDNDVGGEGGDVRTVYKVSRFEKHFENMTGVITH-GFIDFLKLDIIMMRDQVLEKQKIISDVATQLKST-FRIIVNHESMITKPKSSIYSILRMAQPNI--LLTGHWHLSQMFGCDTCLSDDEDMTNW-PYYQRDISKVDGYVDI--DLT-NLIALSEIMVPTCSYRMGVPNMGYGVAVINKTGKMKYTVLWLPAR 279

HSP 2 Score: 93.5893 bits (231), Expect = 2.463e-41
Identity = 43/77 (55.84%), Postives = 53/77 (68.83%), Query Frame = 1 
Query:  130 ILLVGDPQLIGFNYEYKL-FGPINRWDADRFLKSGFRKAVQYTNPDVVVFLGDLFDEGEIATESQFVNYFHRFNDIF 357
            +LLV DPQLIG   E     GPI RWDAD +L+  F +   +T PDV+VFLGD+ DEG  ATES++ +Y HRFN IF
Sbjct:    3 VLLVSDPQLIGIQDEVGFPLGPIIRWDADSYLRKTFSRVYHHTAPDVIVFLGDIMDEGSKATESEYQSYVHRFNSIF 79
[Close]
BLAST of Metallophos domain-containing protein vs. TrEMBL
Match: T1FMY0 (Metallophos domain-containing protein OS=Helobdella robusta OX=6412 GN=20210179 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 1.026e-38
Identity = 45/105 (42.86%), Postives = 67/105 (63.81%), Query Frame = 1 
Query:   46 NEYLLFEYCYFNWSLNYKLKRNIDNEFRILLVGDPQLIGFNYEYKLFGPINRWDADRFLKSGFRKAVQYTNPDVVVFLGDLFDEGEIATESQFVNYFHRFNDIFL 360
            NE + F+     W+    L        +IL V DPQ++G   E+ L G I RWD DR++K+GF +A+++  PDV++FLGDLFDEG  AT++Q+  Y  RF++IF+
Sbjct:   30 NEIISFDLASLQWT---HLNTTDQKTLKILFVADPQIVGLVNEHWLLGYITRWDCDRYIKNGFSRALEHVKPDVIIFLGDLFDEGSRATDTQYATYVERFSNIFV 131

HSP 2 Score: 89.3521 bits (220), Expect = 1.026e-38
Identity = 60/189 (31.75%), Postives = 91/189 (48.15%), Query Frame = 3 
Query:  405 IIIPGDNDIGGEYPSVRNQNIVRRFMKRFNVSYPVVKKIGN-INFIGGDITSNEDFQKTLEF-LIDKYKSTDFHVLFNHDSV-----HMDQKYFKYKPDIPLVITAHSHVSQYKYCEDCSEKYKKWSPTYKLESYEEVVFDLSPGLSKILEITVPTCSYRMGVPYPGYGFLILSDKGMLSYKVLWLPYR 950
            I IPGDND+GGE         ++RF + F      V +IG+ ++ I  +   +   Q  L+   I+K  + +  +  +H  V     +M  K  K  P+I  ++  H H S Y  C+ C  K      +Y  +    ++ D   G S + +ITVPTCSYRMG    GYG++ +     + Y VLWLP R
Sbjct:  139 IYIPGDNDVGGEGFDFLENWKLQRFHRHFPCLNDPVVRIGDFVDVIRMNNYKDRLDQGVLDLNEINKKSTNNIKMFISHLGVIDYYYYMKDKIEKISPNI--IVAGHEHHSSYLKCQKCLTK-NSVETSYSFDENHNILKDKLRGKSTVYQITVPTCSYRMGTAKAGYGYMAIDGNSRMQYTVLWLPQR 324
[Close]
BLAST of Metallophos domain-containing protein vs. TrEMBL
Match: A0A433TKG2 (Metallophos domain-containing protein OS=Elysia chlorotica OX=188477 GN=EGW08_010160 PE=4 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 3.868e-35
Identity = 51/105 (48.57%), Postives = 67/105 (63.81%), Query Frame = 1 
Query:   46 NEYLLFEYCYFNWSLNYKLKRNIDNEFRILLVGDPQLIGFNYEYKL-FGPINRWDADRFLKSGFRKAVQYTNPDVVVFLGDLFDEGEIATESQFVNYFHRFNDIF 357
            NE+L++      W +     RN + E  IL V DPQLIG   E     GPI RWD+DR+L++ F +A Q+T PDVVVFLGDL DEG  AT+ ++++Y  RFN IF
Sbjct:   32 NEFLVYSLQALRW-IKVPTIRNKEEELVILFVADPQLIGIQDEMGFPVGPIARWDSDRYLRNSFSRAYQHTQPDVVVFLGDLLDEGSKATKEEYLSYVDRFNSIF 135

HSP 2 Score: 76.6406 bits (187), Expect = 3.868e-35
Identity = 64/190 (33.68%), Postives = 86/190 (45.26%), Query Frame = 3 
Query:  405 IIIPGDNDIGGEYPSVRNQNIVRRFMKRFNVSYPVVKKIGNINFIGGDITSNEDFQKTLEFLIDKYKSTDFHVLFNHDSVHMDQKYFKYKPDIPLVITAHSHVSQ--------YKYCEDCSEKYKKWSPTYKLESYEEVVFDLSPGLSKILEITVPTCSYRMGVPYPGYGFLILSDKGMLSYKVLWLPYR 950
            +IIPGDNDIGGE   +R    V RF K F    P+    G +N+                F ID  K   F V      V + Q+    K  I + I +    S          +Y  D +    K      +  + E+  D+S  +S + EI VPTCSYRMGVP+ GYG  I++  G++ Y VLWLP R
Sbjct:  144 VIIPGDNDIGGEGADLRTPFKVARFEKHFE---PIE---GIVNY----------------FFIDLIKVNIFFV-----CVRIIQEMLTLKKVIFIAIKSSLFCSNNNNIKLLNMQYNSDLNHWPYKAKDMKNIHGFVEM--DISKTVS-LDEIMVPTCSYRMGVPHMGYGVAIINKSGLMKYAVLWLPSR 303
[Close]
BLAST of Metallophos domain-containing protein vs. TrEMBL
Match: R7UE71 (Metallophos domain-containing protein (Fragment) OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_121495 PE=4 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 7.781e-35
Identity = 62/202 (30.69%), Postives = 99/202 (49.01%), Query Frame = 3 
Query:  405 IIIPGDNDIGGEYPSVRNQNIVRRFMKRF----NVSYPVVKKIGNINFIGGDITSNEDFQKTLEFLIDKYKSTDFHVLFNHDSVHMDQKYFKYKPDI---------PLVITAHSHVSQYKYCEDCSEKYKKWSPTY-------KLESYEEVVFDLSPGLSKILEITVPTCSYRMGVPYPGYGFLILSDKGMLSYKVLWLPYR 950
            + IPGDND+GGE   ++    V+RF + F    +     V K+  I+F+  ++         +E +  +     FH L NH S+       + +P+I          L+I AHSH ++   C +C  + + +   +       +++SYE   F LS   S + E+ VPTCSYRMGVP+ GYG  + +  G + Y +LW P R
Sbjct:  103 VYIPGDNDVGGEAYDMKTDAKVKRFNRNFLHSDSTHTSSVTKVKFIDFLRVNLY--------VELMAQQSSHDAFHFLLNHMSI-----LSRSRPEIHSLLERTKADLIIVAHSHNARLYSCLECLSRPELFRGQFPFKGGIQQIDSYESFHFQLS-NRSVLHEVMVPTCSYRMGVPHMGYGAAVAT--GDVRYSILWTPPR 288

HSP 2 Score: 87.4261 bits (215), Expect = 7.781e-35
Identity = 40/79 (50.63%), Postives = 52/79 (65.82%), Query Frame = 1 
Query:  127 RILLVGDPQLIGFNYEYKLFGPINRWDADRFLKSGFRKAVQYTNPDVVVFLGDLFDEGEIATESQFVNYFHRFNDIFLS 363
            RIL+V DPQL+G   E ++FG I RWD DR+L   F  AV    PDV++FLGDL DEG  A++ ++ +Y  RF  IF S
Sbjct:    2 RILMVADPQLVGIQDESQIFGGITRWDLDRYLSKTFHHAVNNFEPDVILFLGDLLDEGSKASKEEYQSYIKRFRAIFQS 80
[Close]
BLAST of Metallophos domain-containing protein vs. TrEMBL
Match: A0A0L8GZ78 (Metallophos domain-containing protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22025526mg PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 9.968e-34
Identity = 68/194 (35.05%), Postives = 94/194 (48.45%), Query Frame = 3 
Query:  405 IIIPGDNDIGGEYPSVRNQNIVRRFMKRFNVSYPVVKKIGNINFIGGDITSNEDF---QKTLEFLIDKYKSTDFHVLFNHDSVHMDQKYFKY---KPDIP-LVITAHSHVSQYKYCEDCSEKYKK----WSPTYKLESYEEVVFDL-SPGLSKILEITVPTCSYRMGVPYPGYGFLILSDKGMLSYKVLWLPYR 950
            I +PGDNDIGGE    R    + RF + F      VK     +F   D+   E F    K +   + +     F ++ NH+S+    K   Y   KP  P +VI+ H H +    CEDC  +       W  T +  + EE   ++ S   S ++EI VPTCSYRMG P  GYG L+++D G + Y  LWLP R
Sbjct:  145 IYVPGDNDIGGEGFDRRTSEKISRFERYFEYLTGAVK-FRFASFFKLDVRFFEPFLPKHKKILQEVGEEIYMPFRIILNHESISPKLKSHIYPLLKPIRPHMVISGHWHKANLFLCEDCLSQENDDGDYWPVTKRDLTSEENYIEINSDEKSTLVEIMVPTCSYRMGEPKMGYGVLVINDNGNIKYMTLWLPPR 337

HSP 2 Score: 82.0333 bits (201), Expect = 9.968e-34
Identity = 42/114 (36.84%), Postives = 64/114 (56.14%), Query Frame = 1 
Query:   46 NEYLLFEYCYFNWSLNYKLKRNIDNEFRILLVGDPQLIGFNYEYKL-FGPINRWDADRFLKSGFRKAVQYTNPDVVVFLGDLFDEGEIATESQFVNYFHRFNDIFLSGLGEKPV 384
            NEY+L    Y  W+    ++   D +  +LLV DPQ+ G+  E     G  +RWDADR++   F  A  +++PD+++FLGDL DEG IAT  ++  Y+ R  +IF      K +
Sbjct:   33 NEYVLHFAHYLQWA-PIPVETRHDGDMVLLLVSDPQIQGYQREPAFPVGYFSRWDADRYISRSFSMAFLHSSPDIILFLGDLMDEGSIATNEEYELYYQRLQNIFQDAAETKKI 145
[Close]
BLAST of Metallophos domain-containing protein vs. Planmine SMEST
Match: SMESG000077758.1 (SMESG000077758.1)

HSP 1 Score: 387.882 bits (995), Expect = 1.152e-157
Identity = 211/213 (99.06%), Postives = 213/213 (100.00%), Query Frame = 3 
Query:  402 TIIIPGDNDIGGEYPSVRNQNIVRRFMKRFNVSYPVVKKIGNINFIGGDITSNEDFQKTLEFLIDKYKSTDFHVLFNHDSVHMDQKYFKYKPDIPLVITAHSHVSQYKYCEDCSEKYKKWSPTYKLESYEEVVFDLSPGLSKILEITVPTCSYRMGVPYPGYGFLILSDKGMLSYKVLWLPYRXXXXXXXXXXXXXXXXXXXXNRCRFKYIFN 1040
            TIIIPGDNDIGGEYPSVRNQNIVRRFMKRFNVSYPVVKKIGNINFIGGDITSNEDFQKTLEFLIDKYKSTDFHVLFNHDSVHMDQKYFKYKPDIPLVI+AHSHVSQYKYCEDCSEKYKKWSPTYKLESYEEVVFDLSPGLSKILEITVPTCSYRMGVPYPGYGFLILSDKGMLSYKVLWLPYRFYCFGFYCFLLFLMILYIFYNRCRFKY+FN
Sbjct:   94 TIIIPGDNDIGGEYPSVRNQNIVRRFMKRFNVSYPVVKKIGNINFIGGDITSNEDFQKTLEFLIDKYKSTDFHVLFNHDSVHMDQKYFKYKPDIPLVISAHSHVSQYKYCEDCSEKYKKWSPTYKLESYEEVVFDLSPGLSKILEITVPTCSYRMGVPYPGYGFLILSDKGMLSYKVLWLPYRFYCFGFYCFLLFLMILYIFYNRCRFKYMFN 306

HSP 2 Score: 188.734 bits (478), Expect = 1.152e-157
Identity = 91/93 (97.85%), Postives = 92/93 (98.92%), Query Frame = 1 
Query:  106 RNIDNEFRILLVGDPQLIGFNYEYKLFGPINRWDADRFLKSGFRKAVQYTNPDVVVFLGDLFDEGEIATESQFVNYFHRFNDIFLSGLGEKPV 384
            +NIDNEFRILLVGDPQLIGFNYEYKLFGPINRWDADRFLKSGFRKAVQYTNPDVVVFLGDLFDEGEIATESQFVNYFHRFNDIFLSGLGEKP 
Sbjct:    2 KNIDNEFRILLVGDPQLIGFNYEYKLFGPINRWDADRFLKSGFRKAVQYTNPDVVVFLGDLFDEGEIATESQFVNYFHRFNDIFLSGLGEKPT 94
The following BLAST results are available for this feature:
BLAST of Metallophos domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Metallophos domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ZK792.75.576e-2243.02pep chromosome:WBcel235:IV:11694359:11696464:1 gen... [more]
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BLAST of Metallophos domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Mppe5.310e-2037.50gene:FBgn0259985 transcript:FBtr0088024[more]
Mppe5.666e-2037.50gene:FBgn0259985 transcript:FBtr0339583[more]
Mppe5.763e-2037.50gene:FBgn0259985 transcript:FBtr0088025[more]
Mppe6.254e-2037.50gene:FBgn0259985 transcript:FBtr0339582[more]
PGAP51.120e-834.48gene:FBgn0031997 transcript:FBtr0079587[more]
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BLAST of Metallophos domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Metallophos domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Metallophos domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Metallophos domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q9VLR9|MPPE1_DROME1.175e-734.48Metallophosphoesterase 1 homolog OS=Drosophila mel... [more]
sp|Q9UUH0|YKIC_SCHPO3.119e-639.51Uncharacterized protein C630.12 OS=Schizosaccharom... [more]
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BLAST of Metallophos domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0B7AH842.463e-4138.81Metallophos domain-containing protein OS=Arion vul... [more]
T1FMY01.026e-3842.86Metallophos domain-containing protein OS=Helobdell... [more]
A0A433TKG23.868e-3548.57Metallophos domain-containing protein OS=Elysia ch... [more]
R7UE717.781e-3530.69Metallophos domain-containing protein (Fragment) O... [more]
A0A0L8GZ789.968e-3435.05Metallophos domain-containing protein OS=Octopus b... [more]
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BLAST of Metallophos domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Metallophos domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Metallophos domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Metallophos domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Metallophos domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Metallophos domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000077758.11.152e-15799.06SMESG000077758.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028096 ID=SMED30028096|Name=Metallophos domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1165bp
AATTGGAAGTTATTTTTTCTGTTATCTGCGATAGCAATTTTAATTAATGA
GTATTTATTATTTGAATATTGTTACTTCAATTGGAGTTTGAATTACAAGT
TGAAAAGGAATATTGATAATGAATTTAGAATTTTATTAGTGGGTGATCCA
CAGTTAATAGGATTTAACTACGAATACAAATTATTTGGCCCTATTAATAG
ATGGGATGCAGACAGATTTCTCAAATCAGGTTTCAGAAAAGCAGTGCAAT
ACACGAATCCAGATGTCGTGGTTTTCCTCGGTGATCTTTTTGACGAGGGG
GAAATTGCAACTGAATCGCAGTTCGTGAATTACTTTCATCGTTTCAACGA
TATTTTTTTGAGTGGCTTAGGCGAAAAACCGGTAGAATTTTGTGTATTTA
GACAATTATCATACCGGGAGACAATGATATTGGTGGAGAATATCCCTCAG
TCCGAAATCAAAATATAGTTAGGAGATTTATGAAGCGTTTCAATGTATCT
TATCCTGTCGTCAAAAAAATTGGAAATATCAATTTTATTGGCGGTGACAT
TACGTCAAACGAGGACTTTCAAAAAACTTTAGAGTTTTTAATTGATAAAT
ATAAAAGCACGGATTTCCATGTATTATTCAATCATGATTCTGTTCATATG
GATCAAAAATATTTCAAATACAAACCTGACATTCCATTAGTTATAACTGC
TCATTCTCATGTGTCCCAATATAAATATTGTGAGGATTGTAGTGAAAAAT
ACAAAAAGTGGTCACCTACTTACAAATTGGAGTCATATGAAGAAGTTGTG
TTTGATTTATCGCCGGGTTTGTCGAAGATTTTAGAAATAACTGTACCGAC
ATGTAGCTACCGAATGGGCGTTCCATATCCCGGTTATGGGTTTTTGATAT
TGTCTGATAAAGGAATGCTATCTTATAAGGTTTTATGGCTGCCATATCGA
TTTTATTGCTTTGGATTTTATTGTTTCCTGCTTTTCTTGATGATTTTATA
TATCTTTTATAATCGGTGCCGATTTAAATATATATTTAATTGATTTTTTA
ATGTAACATTTTTGGTTTCATTGATGTTGCAGCTCTTAACATAACCACTG
ATTATAATAATATTTAAAAGCTTATTAGAATGGAATTTACAAGATTTGTG
AACTTCCTATGTGCA
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protein sequence of SMED30028096-orf-1

>SMED30028096-orf-1 ID=SMED30028096-orf-1|Name=SMED30028096-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=188bp
MKRFNVSYPVVKKIGNINFIGGDITSNEDFQKTLEFLIDKYKSTDFHVLF
NHDSVHMDQKYFKYKPDIPLVITAHSHVSQYKYCEDCSEKYKKWSPTYKL
ESYEEVVFDLSPGLSKILEITVPTCSYRMGVPYPGYGFLILSDKGMLSYK
VLWLPYRFYCFGFYCFLLFLMILYIFYNRCRFKYIFN*
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protein sequence of SMED30028096-orf-2

>SMED30028096-orf-2 ID=SMED30028096-orf-2|Name=SMED30028096-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=156bp
NWKLFFLLSAIAILINEYLLFEYCYFNWSLNYKLKRNIDNEFRILLVGDP
QLIGFNYEYKLFGPINRWDADRFLKSGFRKAVQYTNPDVVVFLGDLFDEG
EIATESQFVNYFHRFNDIFLSGLGEKPVEFCVFRQLSYRETMILVENIPQ
SEIKI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0006506GPI anchor biosynthetic process
GO:0006888ER to Golgi vesicle-mediated transport
Vocabulary: INTERPRO
TermDefinition
IPR033308PGAP5/Cdc1/Ted1
IPR029052Metallo-depent_PP-like
IPR004843Calcineurin-like_PHP_ApaH
Vocabulary: molecular function
TermDefinition
GO:0016787hydrolase activity
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR033308PGAP5/Cdc1/Ted1PANTHERPTHR13315METALLO PHOSPHOESTERASE RELATEDcoord: 70..178
NoneNo IPR availablePANTHERPTHR13315:SF4METALLOPHOSPHOESTERASE, ISOFORM Ecoord: 70..178
NoneNo IPR availableSUPERFAMILYSSF56300Metallo-dependent phosphatasescoord: 7..84
NoneNo IPR availableTMHMMTMhelixcoord: 155..177