SMED30028092

Overview
NameSMED30028092
Smed IDSMED30028092
Length (bp)1786
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30028092 (SMED30028092) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30028092 (SMED30028092) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30028092 (SMED30028092) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30028092 (SMED30028092) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30028092

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30028092 SmedASXL_002113SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30028092 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.004013:48260..50056 +8690
Homology
[Close]
BLAST of SMED30028092 vs. Planmine SMEST
Match: SMESG000067028.1 (SMESG000067028.1)

HSP 1 Score: 113.235 bits (282), Expect = 1.353e-26
Identity = 90/264 (34.09%), Postives = 138/264 (52.27%), Query Frame = 2 
Query:  308 MENDQECLICYSLIDNKMSFFLENCGHSFCRDCIKELIVNNPTCPMDRISIITLKTINGTKENIINSKDLIEFLKFFNLSNAIKEFNSTFVDFPKIQMKNIFPSLCEESSKEFTDEVFKETIETFKTMSKPSD----GILETYSNLIKRIVSI-------QPENPKTSRLLM-ALLNQYS--EIVYANIMEFNIWSFAIYCINLYKKRKIGRVKFISYSKKISNDITFLHENFCNSRNLFRWLLEKVLVHCKSFHSKELQDLFP 1057
            ME++ EC ICYS +D++   FL +CGHSFC+DC KE +  + TCP+DRI I T+K  +  K  +INSKD I  +K   LS+AI   NS   D P+I  K  F S  ++  K  ++  F   I  F   S+ S      +L T + +   I +I       +    KT+ L+   LL + S  E+++ +  +  I S     I  Y+ +   + + I+Y  K+S  I  L++ F NS +L  WLLE+ +++  S  S +L   FP
Sbjct:    1 MEDNLECFICYSPLDSETRIFLWDCGHSFCKDCPKEFMKTSFTCPLDRIPITTVKIFHDEKVFVINSKDFIGCIK---LSDAIININSELSDLPRINQKTFFLSDFQDLDKAASETEFNVIISDFNEKSQLSQERLGKVLSTLAIIKYNISTISYVLSNFELCTIKTNPLIFYELLKEVSILELIFNH--KITILSEVQNWIESYEDKVKHKEEIINYCLKLSEKINLLNDRFDNSASLCEWLLEQFIMYFNSSLSNKLNCAFP 259
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BLAST of SMED30028092 vs. Planmine SMEST
Match: SMESG000075714.1 (SMESG000075714.1)

HSP 1 Score: 112.849 bits (281), Expect = 1.480e-26
Identity = 91/285 (31.93%), Postives = 140/285 (49.12%), Query Frame = 2 
Query:  308 MENDQECLICYSLIDNKMSFFLENCGHSFCRDCIKELIVNNPTCPMDRISIITLKTINGTKENIINSKDLIEFLKFFNLSNAIKEFNSTFVDFPKI-QMKNIFPSLCEESSKEFTDEVFKETIETFKTMSKPSDGILETYSNL--IKRIVSIQPE---NPKTSRLLM-ALLNQYSEIVYANIMEFNIWSFAIYCINLYKKRKIGRVKFISYSKKISNDITFLHENFCNSRNLFRWLLEKVLVHCKSFHSKELQDLFPNLSITDTQSNLTLLDFPDKYIDLQNKMK 1141
            MENDQECLICYS + N++S    +CGH FCRDC+ E +     CP+DR  +  LK  +GT E  +    +++   F  LSNAI   NS F DFP+I Q K +F       S+   ++VF+   +T+  +S     I+  + ++  I   VS +     N KT ++L+  L  QYS++      E      A  C    ++ KI + +F     +I N +            LF +L+ K+ +    F  ++L+DLFPNL       +      P + ID +N+MK
Sbjct:    1 MENDQECLICYSPLINEISITFISCGHFFCRDCVIESLQIKHICPLDRGEVSLLKINDGTTEQTM----MVD--TFMKLSNAIHNINSKFFDFPQISQNKFLF-------SRFKVNDVFEYYKDTYNRVS-----IISIFYDIKFISNFVSQESSGTWNKKTHQMLVYELQKQYSKLFDHCQSEIQTLFLAENCYKNLQRNKISKEQFQDSITRIINSLNLSENQIETYAKLFEFLMNKIFIFFNKFLKEKLKDLFPNLPTKYNSKDFCEFRIPYRNIDTENEMK 267
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BLAST of SMED30028092 vs. Planmine SMEST
Match: SMESG000067246.1 (SMESG000067246.1)

HSP 1 Score: 110.538 bits (275), Expect = 8.872e-26
Identity = 96/319 (30.09%), Postives = 153/319 (47.96%), Query Frame = 2 
Query:  317 DQECLICYSLIDNKMSFFLENCGHSFCRDCIKELIVN-NPTCPMDRISIITLKTINGTKENIINSKDLIEFLKFFNLSNAIKEFNSTFVDFPKIQMKNIFPSLCEESSKEFTDEVFKETIETFKTMSKPSDGILETYSN--LIKRIVSIQPENPKTSRLLMALLNQYS-EIVYANIMEFNIWSFAIYCINLYKKRKIGRVKFISYSKKISNDITFLHENFCNSRNLFRWLLEKVLVHCKSFHSKELQDLFPNLSITDTQSNLTLLDFPDKYIDLQNKMKQIDILIC----EDKEENIWIKLIHETLQTTVGVFEILLKL 1249
            DQECLICYS + N+ SF  E CGHS+C DC++E +   +  CP+D I I +L   N T   I             N   A+ EF    V   ++Q K  FP        E TDE FKE +  F T SK    I  ++SN  ++  I+          ++L   L     +I +   +  +I   +  C N Y+++ I R +FI Y + +S+ +   ++ +C   NL++ +  K+L     F S +++  FPN S    + NL+L D  D  +   N++K ++  I     ED E+ I+  L++E +    G+ E L K+
Sbjct:    2 DQECLICYSPMSNETSFIFEECGHSYCSDCVEEWMSRKHKRCPLDSIVIESLNVPNRTNTAIAT-----------NTYKAVLEFLDFEVIHTRLQQKLSFPHF-HAVDLETTDEEFKEMVADFFTNSKF--LIYRSHSNDPILSMIIQCNLFYESIYQILTYELQSICLKIEFITKINADIIDNSKDCFNQYREQLIDRAQFIEYLRIVSDMLNKHYQQYCYHVNLYKLIFTKLLEQLNRFISDKMKAFFPNFSTDSRKINLSLSDISD--VPTINRIKALESAISKTNIEDIEQYIFDDLLNENICLFEGLKEFLWKV 304
[Close]
BLAST of SMED30028092 vs. Planmine SMEST
Match: SMESG000019883.1 (SMESG000019883.1)

HSP 1 Score: 105.531 bits (262), Expect = 1.620e-24
Identity = 85/288 (29.51%), Postives = 134/288 (46.53%), Query Frame = 2 
Query:  308 MENDQECLICYSLIDNKMSFFLENCGHSFCRDCIKELIVNNPTCPMDRISIITLKTINGTKENIINSKDLIEFLKFFNLSNAIKEFNSTFVDFPKIQMKNIFPSLCEESSKEFTDEVFKETIETFKTMSKPSDGILETYSNLIKRIVSIQPENPKTSRLLMALLNQYSEI----VYANIMEFNIWSFAIYCINLYKKRKIGRVKFISYSKKISNDITFLHENFCNSRNLFRWLLEKVLVHCKSFHSKELQDLFPNLSITDTQSNLTLLDFPDKYIDLQNKMKQIDILI 1159
            MENDQECLICYS I ++    +  C HSFC DC++E+++ NP CP+DR SI ++K I  +KEN+I  +D+  F+    L     E               IFP + EE++ +  DE+ K               I +   + IK  + I+     +      L  +YS I    + +  +E  I  F   CI  Y+  +I R +       +S +I   H ++ NS    RWL++     C    S+EL+ +F N SI     N   L+     I+  N +K ++++I
Sbjct:    1 MENDQECLICYSPISDETFVTIGFCEHSFCVDCVREMLIANPNCPLDRQSIKSMKMITESKENVILIEDISPFISILKLILNCSEM-------------KIFPVINEETTDKELDEMLKR--------------IEDRNRDAIKLDIDIENLRNLSENKYQLLKFEYSRIKKNQLTSQNIEGCILDFIKTCIRKYQDGEIDRAQLTDGFHHLSKNIDKFHYHYKNSITDIRWLMKIFYNSC--IKSEELKTIFWNCSIV---QNFISLEISTDDIERTNYLKDLELMI 256
[Close]
BLAST of SMED30028092 vs. Planmine SMEST
Match: SMESG000010435.1 (SMESG000010435.1)

HSP 1 Score: 97.4413 bits (241), Expect = 1.794e-21
Identity = 81/284 (28.52%), Postives = 126/284 (44.37%), Query Frame = 2 
Query:  308 MENDQECLICYSLIDNKMSFFLENCGHSFCRDCIKELIVNNPTCPMDRISIITLKTINGTKENIINSKDLIEFLKFFNLSNAIKEFNSTFVDFPKIQMKNIFPSLCEESSKEFTDEVFKETIETFKTMSKPSDGILETYSNLIKRIVSIQPENPKTSRLLMALLNQYSEIVYANIMEFNIWSFAIYCINLYKKRKIGRVKFISYSKKISNDITFLHENFCNSRNLFRWLLEKVLVHCKSFHSKELQDLFPNLSITDTQSNLTLLDFPDKYIDLQNKMKQIDILI 1159
            M N +ECLICYS + ++    +  C HSFC DC+ E++V  P CP DR SI ++K I G+KEN I+ +D+  F+    L                I  KNIF  + EES+    +E+ ++ I+  K     S+   +       RI+  Q                       N +E+NI  F I CI+ Y    I R + +SY   +S  I  +  ++  S    RWL++     C    S++ + L  + SI     N    D     I+  N MK ++++I
Sbjct:    1 MANKKECLICYSPMSDETVVTIGFCEHSFCVDCLVEILVTKPICPFDRKSIKSMKMITGSKENFISIEDISSFISILKL----------------ISEKNIFHIINEESTDADLNEILEQNIDIIKNYHNLSENKFQLLKFEFFRIIMKQ--------------------CAVNNIEYNILEFRIDCISYYLDEIIDRGQLMSYFHLLSQYINKIQYHYKASITDIRWLMKLFYNSCNK--SEQPETLLWDCSI---MKNFVYPDISMDNIERTNYMKNLELVI 243
The following BLAST results are available for this feature:
BLAST of SMED30028092 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028092 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028092 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028092 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028092 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028092 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028092 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028092 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028092 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028092 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028092 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028092 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028092 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30028092 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000067028.11.353e-2634.09SMESG000067028.1[more]
SMESG000075714.11.480e-2631.93SMESG000075714.1[more]
SMESG000067246.18.872e-2630.09SMESG000067246.1[more]
SMESG000019883.11.620e-2429.51SMESG000019883.1[more]
SMESG000010435.11.794e-2128.52SMESG000010435.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30028092 ID=SMED30028092|Name=SMED30028092|organism=Schmidtea mediterranea sexual|type=transcript|length=1786bp
TATAGATAAATACTTCATAAGTAATTTATCAAAATTTAAAAAAATATAAT
ATTTACAAATTTAAAATTGAATAAAAATTTAAATAAAAATTAATATTATA
TTTAAATTTTAATCAAATTTATATTTTATTTCAAATTCCATTCTTTTTGG
ATTTTAAGATTTATAAATTATTTTAAACGTTGAATAAATATTGAAAATCA
ATTCATTAATTTTTTAAATTTTATTGAAAAAATAATTATTTTATTAATTG
AAATTTTTATATTTTGTTTAAATATTGGCTATTATTTCACTATATTCCAA
TAAATTAATGGAAAACGACCAAGAATGTTTGATATGCTATTCACTAATCG
ATAATAAAATGAGTTTTTTTCTGGAAAACTGTGGACATTCATTTTGTAGA
GATTGTATTAAAGAATTGATTGTTAATAATCCAACTTGTCCAATGGATAG
AATATCGATTATCACTTTGAAAACAATTAATGGGACCAAAGAAAATATCA
TAAATTCAAAAGATTTAATTGAATTTTTGAAATTTTTTAATTTATCAAAT
GCCATAAAGGAATTTAATTCGACTTTTGTTGATTTCCCAAAGATACAGAT
GAAGAATATTTTCCCTTCTTTATGTGAAGAATCTAGTAAAGAATTTACAG
ATGAAGTGTTTAAAGAAACAATCGAAACCTTCAAGACAATGTCAAAACCA
TCAGATGGTATTTTAGAAACATATTCCAACTTGATTAAACGTATTGTTAG
TATACAACCCGAAAACCCGAAAACATCCCGTTTGCTAATGGCACTGTTAA
ACCAATATAGTGAAATAGTATACGCAAATATAATGGAATTCAATATATGG
AGTTTCGCAATTTACTGTATAAATTTGTATAAAAAAAGAAAAATAGGTCG
AGTAAAATTTATTTCTTATTCTAAAAAGATATCTAACGATATAACATTTC
TACACGAAAACTTCTGCAATTCTAGAAATCTGTTTCGATGGTTATTGGAA
AAAGTATTAGTTCACTGCAAAAGTTTCCATTCCAAAGAATTGCAAGATTT
ATTTCCAAATTTATCAATTACTGACACTCAGTCTAATTTGACTTTATTAG
ATTTTCCCGATAAATATATTGACTTGCAAAACAAGATGAAACAAATAGAC
ATCTTGATATGTGAAGATAAAGAAGAAAATATTTGGATAAAACTTATTCA
TGAAACTTTGCAAACGACTGTTGGTGTGTTTGAAATACTGTTAAAATTAG
GAGAATCTATTTTTGGTGATCCTGAGTTTTCAAATCACGGTTCAGTATAT
TTCATAACTTTTCAACAAAGACTAGTTAACATTTTACAACGTATGTCTCG
ACAATGTTCAAAACTCTGGACATATATTTCTTTGAATCAATCTCAAAATA
TACATATTTTCTATCTTAGTCTTAGTGGTATTATGAATAGTATCAAAAAC
ATGATAAAATATATTATCGCGAATCTTACAGATATTAAAAATGTAACTGG
TGATTTCAAAATAAAACTTAAAATTGGATGTACTATCATTTTAGATGAAA
TTCAATGTCATTGTGAAAATTTGCATCTAACAAAAATTGAAAATATTCTA
GAATTAGTGAAACGATTTCGTCAAATTATGGCTGAAATTTAACTTATTTC
ACGATTAAACTTTGTATAGAATAAATTTAATATTAAACTGTCACTAAATT
GATCAATTTATTTTATTTGACCTACTTTTTTTGTATTGGACTTACTTTTT
TTGTATTGGACATACTTTATTTGTATTTGACCTACT
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protein sequence of SMED30028092-orf-1

>SMED30028092-orf-1 ID=SMED30028092-orf-1|Name=SMED30028092-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=445bp
MENDQECLICYSLIDNKMSFFLENCGHSFCRDCIKELIVNNPTCPMDRIS
IITLKTINGTKENIINSKDLIEFLKFFNLSNAIKEFNSTFVDFPKIQMKN
IFPSLCEESSKEFTDEVFKETIETFKTMSKPSDGILETYSNLIKRIVSIQ
PENPKTSRLLMALLNQYSEIVYANIMEFNIWSFAIYCINLYKKRKIGRVK
FISYSKKISNDITFLHENFCNSRNLFRWLLEKVLVHCKSFHSKELQDLFP
NLSITDTQSNLTLLDFPDKYIDLQNKMKQIDILICEDKEENIWIKLIHET
LQTTVGVFEILLKLGESIFGDPEFSNHGSVYFITFQQRLVNILQRMSRQC
SKLWTYISLNQSQNIHIFYLSLSGIMNSIKNMIKYIIANLTDIKNVTGDF
KIKLKIGCTIILDEIQCHCENLHLTKIENILELVKRFRQIMAEI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR001841Znf_RING
IPR013083Znf_RING/FYVE/PHD
IPR017907Znf_RING_CS
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0008270zinc ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 7..47
e-value: 2.4E-5
score: 33.7
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 6..48
e-value: 1.9E-8
score: 34.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 7..48
score: 12.386
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 1..89
e-value: 1.2E-12
score: 49.6
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 25..34
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 2..55