SMED30028058
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30028058 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of SMED30028058 vs. Planmine SMEST
Match: SMESG000056175.1 (SMESG000056175.1) HSP 1 Score: 714.146 bits (1842), Expect = 0.000e+0 Identity = 350/355 (98.59%), Postives = 353/355 (99.44%), Query Frame = -2 Query: 767 MALESLPLALTIASVAFGLLLVSGLVWLIPVYIQKQKSKTRKKEMQKKVNEELVRNIRYRSPSTVSTFPQNSKINDYGATQMRQLEKSERESFIVPIEEKAEVPSIAITCEDELTEGAVSESETQFKVSDGVLDKISLINSKRRFSSIVFTHSDELKVLSEDANLNTGEEKKERRASMSLFETCKLACPELTQNMGQSTEHLNQLVLSDSESYGKLYFMLSQPDNDNKSLECVVIKITDIVNMKKKHYQLQMELFVENSKLIYSNQTDIQICVGEVVNFDESFTVDIDGKVQFNDSNENFVLVFTLVQFDSKKIKSQKFGVIRFDLLGLIDGQILSMNGQEQFVICRNFITLTEK 1831 MALESLPLALTIASVAFGLLLVSGLVWLIPVYIQKQKSKTRKKEMQKKVNEELV+NIRYRSPSTVSTFPQNSKINDYGATQMRQLEKSERESFIVPIEEK EVPSIAITCEDELTEGAVSESETQFKVSDGVLDKISLINSKRRFSSIVFTHSDELKVLSEDANLNTGEEKKERRASMSLFETCKLACPELTQNMGQSTEHLNQLVLSD+ESYGKLYFMLSQPDNDNKSLECVVIKITDIVNMKKKHYQLQMELFVENSKLIYSNQTDIQ CVGEVVNFDESFTVDIDGKVQFNDSNENFVLVFTL+QFDSKKIKSQKFGVIRFDLLGLIDGQILSMNGQEQFVICRNFITLTEK Sbjct: 1 MALESLPLALTIASVAFGLLLVSGLVWLIPVYIQKQKSKTRKKEMQKKVNEELVKNIRYRSPSTVSTFPQNSKINDYGATQMRQLEKSERESFIVPIEEKTEVPSIAITCEDELTEGAVSESETQFKVSDGVLDKISLINSKRRFSSIVFTHSDELKVLSEDANLNTGEEKKERRASMSLFETCKLACPELTQNMGQSTEHLNQLVLSDAESYGKLYFMLSQPDNDNKSLECVVIKITDIVNMKKKHYQLQMELFVENSKLIYSNQTDIQTCVGEVVNFDESFTVDIDGKVQFNDSNENFVLVFTLLQFDSKKIKSQKFGVIRFDLLGLIDGQILSMNGQEQFVICRNFITLTEK 355
BLAST of SMED30028058 vs. Planmine SMEST
Match: SMESG000056172.1 (SMESG000056172.1) HSP 1 Score: 278.485 bits (711), Expect = 2.496e-91 Identity = 135/135 (100.00%), Postives = 135/135 (100.00%), Query Frame = -2 Query: 278 MEVKDIASSKPYSGLSVRITLASEGKVLDSRKSKVIKKKYQTEDGIFKVDESFEFNLSLQKSIIKDNSKSLAKFTSQDTFVTLFLCGRNNIGFSHVIGKCVLGPIQFADGTGLLHWQKGNNNLGEIVYQWHYLKN 682 MEVKDIASSKPYSGLSVRITLASEGKVLDSRKSKVIKKKYQTEDGIFKVDESFEFNLSLQKSIIKDNSKSLAKFTSQDTFVTLFLCGRNNIGFSHVIGKCVLGPIQFADGTGLLHWQKGNNNLGEIVYQWHYLKN Sbjct: 1 MEVKDIASSKPYSGLSVRITLASEGKVLDSRKSKVIKKKYQTEDGIFKVDESFEFNLSLQKSIIKDNSKSLAKFTSQDTFVTLFLCGRNNIGFSHVIGKCVLGPIQFADGTGLLHWQKGNNNLGEIVYQWHYLKN 135
BLAST of SMED30028058 vs. Planmine SMEST
Match: SMESG000058817.1 (SMESG000058817.1) HSP 1 Score: 87.4261 bits (215), Expect = 5.443e-18 Identity = 115/502 (22.91%), Postives = 215/502 (42.83%), Query Frame = -2 Query: 293 IQKQKSKTRKKEMQKKVNEELVRNIRYRSPSTVSTFPQNSKINDYGATQMRQLEKSERESFIVPIEEKAEVPSIAITCEDELTEGAVSESETQFKVSDGVLDKISLINSK-RRFSSIVFTHSDEL--------------KVLSEDANLNTGEEKKERRASMSLF-ETCKLACPELTQNMGQSTEHLNQLVLSDSESYGKLYFMLSQPDNDNKSLECVVIKITDIVNMKKKHYQLQMELFVENSKLIYSNQTDIQ--ICVGEVVN--FDESFTVDIDGKVQFNDSNENFVLVFTLVQFDSKKIKSQK-FGVIRFDLLGLIDGQILSMNGQEQFVICRNFITLTEKEKFKERSGNALVFLRFSKLCPLNFVIGVMEVKDIASSKPYSGLSVRITLASEGKVLDSRKSKVIKKKYQTEDGIFKVDESFEFNLSLQKSIIKDNSKSLAKFTSQDTFVTLFLCGRNNIGFSHVIGKCVLGPIQFADGTGLLHWQKGNNNLGEIVYQW 1735 ++K + RK+ +KKV EEL N+RY +P ++S A Q R S R++ P+I+I C+ +E + S T++ I LI +K RR+S + F+ + + ++ E + E + RR S + + + + E+ ++ ST+ ++ G+LYF++ N NKSL + + I ++ + ++V+ + L +Q CV + N F+++FT I Q L + +K++S + G + FDL ++ + QE I R + K R G+ LV L+ S + + V E +++ + VR +L + KV+ ++K + +K+ + ++ F+F ++ + I D + + L C R+ + FSH+IG+C++G IQ+ +G GLL W + + G V QW Sbjct: 36 LKKSRISNRKQFSRKKVVEELHHNVRYNTPDSLSV--------SLAAVQPRS---SRRQTL----------PAISI-CQQN-SEFS-SSGHTRY-------GSIELIRTKVRRYSEVGFSRPEPVEKSRKISLDSEALSRICEESTEMYEYESSRSRRGSTASYADKDSTSHSEIDVDLYSSTDDVSSQSPMCGCGLGRLYFIV----NHNKSLNNLYVTIVKATYVRSSVVKETCCVYVKITLLDAECTYPVQRSSCVTSMGNPMFNQTFTFHITSIPQ----------QLQLTVKEVRKLESNRVIGYVWFDLSCMLKDNRAMITSQE---ISREIFG---ENKLYCRRGDVLVNLKVSANDSIEINVCVAEARNLIVRNQNISVYVRASLLIKHKVVQTKKGQPVKRSISSPS----FNDRFKFVITNKDISILD-----------ECCIILCFCSRSRVRFSHLIGRCIIGNIQYTEGEGLLQWTESISKPGTSVSQW 471
BLAST of SMED30028058 vs. Planmine SMEST
Match: SMESG000060758.1 (SMESG000060758.1) HSP 1 Score: 63.1586 bits (152), Expect = 3.092e-10 Identity = 78/326 (23.93%), Postives = 149/326 (45.71%), Query Frame = -2 Query: 290 MGQSTEHLNQLVLSDSESYGKLYFMLSQPDNDNKSLECVVIKITDIVNM---KKKHYQLQMELFVENSKLIYSNQTDIQICVGEVVNFDESFTVDIDGKVQFNDSNENFVLVFTLVQFDSKKIK--SQKFGVIRFDLLGLIDGQILSMNGQEQFVICRNFITLTEKEKFKERSGNALVFLRFSKLCPLNFV-IGVMEVKDIASSKPYSGLSVRITLASEGKVLDSRKSKVIKKKYQTEDGIFKVDESFEFNLSLQKSIIKDNSKSLAKFTSQDTFVTLFLCGRNNIGFSHVIGKCVLGPIQFADGTGLLHWQKGNNNLGEIVYQWH 1249 + +S N L+LS +L+F +S ND+ + V IK + V+ K + +++E++ E S IYS+ T +G + F+++ ++ + F+ +++L F K+ K ++ G + F L L I S N ++F +T K E G LV + F K V +G++ ++ +G+ ++ + + S ++ IK ++ +F + + F+ + +S SL+K T++ + +CGRN G S +IG+C+ GPI ++D G HW + +VY+WH Sbjct: 156 VDKSYNFFNSLILS------RLHFTISF--NDSAKIMYVNIKKVNNVSCTFEKTINCSIKVEVYDERSNRIYSSVTATVTSMGNPI-FEQTLAFEV---------HSTFINLWSLRFFILKREKKINKNIGSVAFSLDCL--SPIRSPNMDQEFSR-----MITYDFKNPENKGIVLVSVSFHKELDKYIVSVGIIRALNLPLDNNDNGVYAKMIFMDLEQEIKSERTITIKT--DIKNPLFPLAQ---FSHEISES-------SLSK-----TWLVVNVCGRNVFGISRIIGRCLFGPIAYSDHDGSSHWSDAITLIDRVVYKWH 439
BLAST of SMED30028058 vs. Planmine SMEST
Match: SMESG000036721.1 (SMESG000036721.1) HSP 1 Score: 48.9062 bits (115), Expect = 1.503e-6 Identity = 36/160 (22.50%), Postives = 69/160 (43.12%), Query Frame = -2 Query: 290 RSGNALVFLRFSKLCPLNFV-IGVMEVKDIASSKPYSGLSVRITLASEGKVLDSRKSKVIKK----KYQTEDGIFKVDESFEFNLSLQKSIIKDNSKSLAKFTSQDTFVTLFLCGRNNIGFSHVIGKCVLGPIQFADGTG-LLHWQKGNNNLGEIVYQWH 751 R G L+ L +S+ F+ + + K++ + + + R T+ K + +++S +K+ Y + +F VD K + V + +C R+ IGFSH++GK +LG + + D L+HW+ + V +WH Sbjct: 17 RRGEILISLMYSEHQSSAFLSVNLHRAKNLQIRQHDNSVFARCTMLVGNKPIKTKRSITVKQDISAPYVDLESVFNVD----------------------KLNIMEVGVVIHICSRSKIGFSHLLGKLILGSVMYTDDKSVLIHWKDMISYQNRTVTKWH 154 The following BLAST results are available for this feature:
BLAST of SMED30028058 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30028058 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30028058 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30028058 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30028058 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30028058 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30028058 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30028058 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30028058 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30028058 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30028058 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30028058 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30028058 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30028058 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30028058 ID=SMED30028058|Name=SMED30028058|organism=Schmidtea mediterranea sexual|type=transcript|length=1874bpback to top protein sequence of SMED30028058-orf-1 >SMED30028058-orf-1 ID=SMED30028058-orf-1|Name=SMED30028058-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=519bp MALESLPLALTIASVAFGLLLVSGLVWLIPVYIQKQKSKTRKKEMQKKVNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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