Retinal homeobox protein Rax

Overview


Name	Retinal homeobox protein Rax
Smed ID	SMED30028057
Length (bp)	1510

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Retinal homeobox protein Rax (SMED30028057) t-SNE clustered cells

Violin plots show distribution of expression levels for Retinal homeobox protein Rax (SMED30028057) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Retinal homeobox protein Rax (SMED30028057) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Retinal homeobox protein Rax (SMED30028057) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30028057

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 12

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
head region	SMED30028057	SMESG000019089.1	SmedASXL_008135	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
pharynx	SMED30028057	SMESG000019089.1	dd_Smed_v4_6404_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30028057	SMESG000019089.1	dd_Smed_v4_6404_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
epidermis	SMED30028057	SMESG000019089.1	dd_Smed_v4_6404_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30028057	SMESG000019089.1	dd_Smed_v4_6404_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neuron	SMED30028057	SMESG000019089.1	dd_Smed_v4_6404_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchyma	SMED30028057	SMESG000019089.1	dd_Smed_v4_6404_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30028057	SMESG000019089.1	Contig31737	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30028057	SMESG000019089.1	Contig31737	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
head region	SMED30028057	SMESG000019089.1	dd_Smed_v6_6404_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
head region	SMED30028057	SMESG000019089.1	OX_Smed_1.0.09926	ox_Smed_v2	PMID:24238224 Kao et al., 2013
epidermis	SMED30028057	SMESG000019089.1	dd_Smed_v4_6404_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Homology

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BLAST of Retinal homeobox protein Rax vs. Ensembl Human
Match: PPP1R32 (protein phosphatase 1 regulatory subunit 32 [Source:HGNC Symbol;Acc:HGNC:28869])

HSP 1 Score: 110.153 bits (274), Expect = 1.693e-25
Identity = 105/415 (25.30%), Postives = 183/415 (44.10%), Query Frame = 3

Query:   90 IGPVTKHIKASSGGETYALKHYCTTNENSYGSGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPKIGEIIKTNYKSITQKDYNGFKIPNGTETLPLSTLYQPSGFVRE-PGVHIPTEIQLQSQFINGLLQKSPANIPG---KHKPLLYQTYPKDPVMLENSGYGPFYMSTEYKTQYDGKQCRD-------LGPGRIGNLEMTGFTENNKTEEPLEGRPDACMCNHEAFGNPPYNCRPMGHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLEWIRRNDPAEYINAINCNRIPTMMNAVYQGKQIQPIGNCDFRTKTGNLEVTGHISNQDIFVQT---RDKPERFITNSMTQFYDPSLAGSERNGHINGQVQPTMENGFS 1292
            +G V+ ++K SSGG T  LK Y T+   +YG   E F PR G+H G GY  N +  +   + L+ +DNP  GE  + +++S+  + Y   ++P+G   LP S     SG+ RE P    PT+   +  F     +  P  I G   +  PLL+Q   +DP+  EN  +GP +M++EY ++Y     RD       L    IG  E +GFT+ +  + P+  +P +     +    P       G S+  + + P    +G E LP +   +     F++GN+ +   +         + S ++  + + + R                       Q   +  +G    R   G  E TG   N  ++V++    D+ +R++T     +++    G ++ G   G +QP M  G++
Sbjct:    7 LGVVSPYVKMSSGGYTDPLKFYATSYCTAYGR--EDFKPRVGSHVGTGYKSNFQPVVSCQASLEALDNPARGEQAQDHFQSVASQSYRPLEVPDGKHPLPWSMRQTSSGYGREKPSAGPPTKEVRKVHFDT--QEHGPQAITGLEPREVPLLHQQQGQDPLERENFRHGPRFMTSEYNSKY----LRDPLDQPDFLQKKSIGAKEGSGFTKQSH-QSPIVFQPPSQALPGDPALLP-------GQSVTKSDFLPKTHLHGDEFLPVLARGSKRETAFSRGNERI---LNPRVPPPCPEPSSVSHQQFQPLHR----------------------MQQTNVALLG----RETVGKKEPTGFSLNNPMYVRSPCDPDRDQRYLTTYNQGYFENIPKGLDQEGWTRGGIQPQMPGGYA 376

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BLAST of Retinal homeobox protein Rax vs. Ensembl Human
Match: PPP1R32 (protein phosphatase 1 regulatory subunit 32 [Source:HGNC Symbol;Acc:HGNC:28869])

HSP 1 Score: 102.064 bits (253), Expect = 6.827e-23
Identity = 105/415 (25.30%), Postives = 176/415 (42.41%), Query Frame = 3

Query:   90 IGPVTKHIKASSGGETYALKHYCTTNENSYGSGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPKIGEIIKTNYKSITQKDYNGFKIPNGTETLPLSTLYQPSGFVRE-PGVHIPTEIQLQSQFINGLLQKSPANIPG---KHKPLLYQTYPKDPVMLENSGYGPFYMSTEYKTQYDGKQCRD-------LGPGRIGNLEMTGFTENNKTEEPLEGRPDACMCNHEAFGNPPYNCRPMGHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLEWIRRNDPAEYINAINCNRIPTMMNAVYQGKQIQPIGNCDFRTKTGNLEVTGHISNQDIFVQT---RDKPERFITNSMTQFYDPSLAGSERNGHINGQVQPTMENGFS 1292
            +G V+ ++K SSGG T  LK Y T+   +YG   E F PR G+H G GY  N +  +   + L+ +DNP  GE  + +++S+  + Y   ++P+G   LP S     SG+ RE P    PT+   +  F     +  P  I G   +  PLL+Q   +DP+  EN  +GP +M++EY ++Y     RD       L    IG  E +GFT+ +  + P+                PP    P             G P G E LP +   +     F++GN+ +   +         + S ++  + + + R                       Q   +  +G    R   G  E TG   N  ++V++    D+ +R++T     +++    G ++ G   G +QP M  G++
Sbjct:    7 LGVVSPYVKMSSGGYTDPLKFYATSYCTAYGR--EDFKPRVGSHVGTGYKSNFQPVVSCQASLEALDNPARGEQAQDHFQSVASQSYRPLEVPDGKHPLPWSMRQTSSGYGREKPSAGPPTKEVRKVHFDT--QEHGPQAITGLEPREVPLLHQQQGQDPLERENFRHGPRFMTSEYNSKY----LRDPLDQPDFLQKKSIGAKEGSGFTKQSH-QSPI-------------VFQPPSQALP-------------GDP-GDEFLPVLARGSKRETAFSRGNERI---LNPRVPPPCPEPSSVSHQQFQPLHR----------------------MQQTNVALLG----RETVGKKEPTGFSLNNPMYVRSPCDPDRDQRYLTTYNQGYFENIPKGLDQEGWTRGGIQPQMPGGYA 356

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BLAST of Retinal homeobox protein Rax vs. Ensembl Zebrafish
Match: ppp1r32 (protein phosphatase 1, regulatory subunit 32 [Source:ZFIN;Acc:ZDB-GENE-120913-1])

HSP 1 Score: 65.855 bits (159), Expect = 2.834e-11
Identity = 71/259 (27.41%), Postives = 103/259 (39.77%), Query Frame = 3

Query:  141 ALKHYCTTNENSYGSGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPKIGEIIKTNYKSITQKDYNGFKIPNGTETLPLSTLYQPSGFVREPGVHIPTEIQLQSQFINGLLQKSPANIPGKHKPLLYQTYPKDPVMLENSGYGPFYMSTEYKTQYDGKQCR--DLGPGR--IGNLEMTGFTENNKTEEPLEGRPDACMCNHEAFGNPPY----NCRPMGHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTK 893
            ++ HYCT+   SYG   E F P  G H G GY  N+R  ++Y+S LD  DNP+ G  +  +++S +++ Y     P+GTE L  S   +     RE G      +QLQSQ                            P +   S        TEYK  Y     R  D    R  IG++E +G+TE             A +  H     P Y    + R    S+  T +       GR+  P + +R+    GFT+
Sbjct:   25 SIDHYCTSYRQSYGK--ELFQPCLGYHHGTGYSANHRPVLYYSSRLDDYDNPQFGFSLLDSFESQSKRHYQRLVQPDGTEPLACSGSRR-----RESGY-----LQLQSQ----------------------------PRLRTAS------CQTEYKGAYIPHSLRVVDASDDRVLIGHIEDSGYTEG------------ANLQLHTFL--PKYVNMDDARRTHESVMRTDFLAGSFLQGRKAFPKLASRSNRETGFTR 223

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BLAST of Retinal homeobox protein Rax vs. Ensembl Xenopus
Match: rnf11 (ring finger protein 11 [Source:Xenbase;Acc:XB-GENE-852608])

HSP 1 Score: 122.479 bits (306), Expect = 4.891e-30
Identity = 119/417 (28.54%), Postives = 180/417 (43.17%), Query Frame = 3

Query:   90 IGPVTKHIKASSGGETYALKHYCTTNENSYG------SGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPKIGEIIKTNYKSITQKDYNGFKIPNGTETLPLSTLYQP-SGFVREPGVHIPTEIQLQSQFINGL--LQKSPANIPGKHKPLLYQTYPKDPVMLENSGYGPFYMSTEYKTQYDGKQCRDLGPGR---IGNLEMTGFTENNKTEEPLEGRPDACMCNHEAFGN---PPYNCRPMGHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLEWIRRNDPAEYINAINCNRIPTMMNAVYQGKQIQPIGNCDFRTKTGNLEVTGHISNQDIFV-QTRDKPERFITNSMTQFYDPSLAGSERNGHINGQVQPTMENGFS 1292
            +G ++  I+ S GG   AL  YCTT  NSYG       G+ H +    +H   GY  N R  ++Y+  LD +DNP +G +++ NY SIT +D+   K+P GTE LP  TL+ P SG+ +   +  P    ++S   +         A +P  H+PLL     K     EN  +GP +MSTEYK+++        G  R   +G  E +GFTE    E          + N++ +     P    R MG S+  T + PL    GRE LP +  +A   +GFT+    V                         +  N P       N NR           + +  +G      K    E +G+  N  ++V   +  P++++TN   +F DP   G +R G   G +Q    +GFS
Sbjct:    6 LGVMSPQIQGSLGGSCDALNFYCTTYNNSYGLSDIPTGGFSHRSAGL-SHRRTGYQSNLRPAVYYSPSLDRLDNPNMGLMLRNNYTSITNRDFGPLKVPTGTEPLPY-TLHPPNSGYHKSSEITNPKFRAVKSVHFDTRDHCASGVAGVPPVHRPLLTAQKGKGAPETENFYHGPAFMSTEYKSRFTPGNGHRPGFPRSAVVGPTENSGFTEGLNLE----------IINYDPYSQHRAPKGYHRMMGQSMTKTDFLPLTTLQGRELLPALAKKAERDSGFTREANTV-------------------------LSVNTPHGTQPGDNGNR-----------QNLDMLGQVSVGIK----EPSGYSQNNFLYVQPQQQPPQQYLTNYNMRFTDPKPVGKDREGWTRGGIQKQHFSGFS 370

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BLAST of Retinal homeobox protein Rax vs. Ensembl Mouse
Match: Ppp1r32 (protein phosphatase 1, regulatory subunit 32 [Source:MGI Symbol;Acc:MGI:1915002])

HSP 1 Score: 127.102 bits (318), Expect = 1.447e-31
Identity = 127/436 (29.13%), Postives = 199/436 (45.64%), Query Frame = 3

Query:   90 IGPVTKHIKASSGGETYALKHYCTTNENSYGSGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPKIGEIIKTNYKSITQKDYNGFKIPNGTETLPLSTLYQPSGFVRE---PGVHIPT--EIQLQSQFINGLLQKSPANIPG---KHKPLLYQTYPKDPVMLENSGYGPFYMSTEYKTQY--DGKQCRDLGPGR-IGNLEMTGFTENNKTEEPLEGRPDACMCNHEAF-GNPPYNCRPMGHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLEWIRRNDPAEYINAINCNRIPTMMNAVYQGKQIQPIGNCDF--RTKTGNLEVTGHISNQDIFVQT---RDKPERFITNSMTQFYDPSLAGSERNGHINGQVQPTMENGFS-REVKLHKIEPQMDALTN 1343
            +G V+ ++K SSGG +  LK Y T+   +YG   E F PR G+H G GY  N R  + Y  HLD +DNP IG+ I+   KS+T + Y+  ++P+G + LP +     S + RE   PG H     ++   +Q         P  I G   K  PL++Q   K     ENS YGP +M++EY ++Y  +     DL   + IG+ E TGFTE + T+ P+  +P +     +AF G+P  +    G SI  + Y P+  P G + LP +   +    GF++ N+       ++P RV   A                             +M +  YQ  Q     N     R   GN E TG   N   +V++   +D+ +R++T     +++    G +R G   G +QP     ++  E+  H +   MD+  N
Sbjct:    7 LGVVSPYVKMSSGGCSDPLKFYATSYCTAYGR--EEFKPRLGSHVGTGYKSNYRPLVSYQPHLDTLDNPAIGQQIRDTSKSVTSQSYSPLEVPDGKQPLPWNLHQTTSSYGREKLNPGPHSKEVRKVHFDTQ------DHGPQTITGLEPKEVPLIHQQQGKGSTEWENSHYGPRFMTSEYNSKYIKESPNHPDLLLKKTIGSKEETGFTEES-TKNPIVFQPPS-----QAFPGDPVLHP---GRSITKSDYLPVTHPQGSDFLPVLSRGSDRDTGFSRVNERT-----LNP-RVPTPAPQSA-------------------------SMSHRSYQPPQRMQQTNVALLGRESVGNKEPTGFTLNNPSYVRSSYEQDRDQRYLTTYNQGYFENIPKGLDREGWTRGGIQPQKAGAYALSELNNHTL---MDSTPN 391

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Query:   90 IGPVTKHIKASSGGETYALKHYCTTNENSYGSGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPKIGEIIKTNYKSITQKDYNGFKIPNGTETLPLSTLYQPSGFVRE---PGVHIPT--EIQLQSQFINGLLQKSPANIPG---KHKPLLYQTYPKDPVMLENSGYGPFYMSTEYKTQY--DGKQCRDLGPGR-IGNLEMTGFTENNKTEEPLEGRPDACMCNHEAF-GNPPYNCRPMGHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLEWIRRNDPAEYINAINCNRIPTMMNAVYQGKQIQPIGNCDF--RTKTGNLEVTGHISNQDIFVQT---RDKPERFITNSMTQFYDPSLAGSERNGHINGQVQPTMENGFS-REVKLHKIEPQMDALTN 1343
            +G V+ ++K SSGG +  LK Y T+   +YG   E F PR G+H G GY  N R  + Y  HLD +DNP IG+ I+   KS+T + Y+  ++P+G + LP +     S + RE   PG H     ++   +Q         P  I G   K  PL++Q   K     ENS YGP +M++EY ++Y  +     DL   + IG+ E TGFTE + T+ P+  +P +     +AF G+P  +    G SI  + Y P+  P G + LP +   +    GF++ N+       ++P RV   A                             +M +  YQ  Q     N     R   GN E TG   N   +V++   +D+ +R++T     +++    G +R G   G +QP     ++  E+  H +   MD+  N
Sbjct:    7 LGVVSPYVKMSSGGCSDPLKFYATSYCTAYGR--EEFKPRLGSHVGTGYKSNYRPLVSYQPHLDTLDNPAIGQQIRDTSKSVTSQSYSPLEVPDGKQPLPWNLHQTTSSYGREKLNPGPHSKEVRKVHFDTQ------DHGPQTITGLEPKEVPLIHQQQGKGSTEWENSHYGPRFMTSEYNSKYIKESPNHPDLLLKKTIGSKEETGFTEES-TKNPIVFQPPS-----QAFPGDPVLHP---GRSITKSDYLPVTHPQGSDFLPVLSRGSDRDTGFSRVNERT-----LNP-RVPTPAPQSA-------------------------SMSHRSYQPPQRMQQTNVALLGRESVGNKEPTGFTLNNPSYVRSSYEQDRDQRYLTTYNQGYFENIPKGLDREGWTRGGIQPQKAGAYALSELNNHTL---MDSTPN 391

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BLAST of Retinal homeobox protein Rax vs. UniProt/SwissProt
Match: sp|Q148A4|PPR32_MOUSE (Protein phosphatase 1 regulatory subunit 32 OS=Mus musculus OX=10090 GN=Ppp1r32 PE=1 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 9.003e-31
Identity = 129/439 (29.38%), Postives = 203/439 (46.24%), Query Frame = 3

Query:   90 IGPVTKHIKASSGGETYALKHYCTTNENSYGSGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPKIGEIIKTNYKSITQKDYNGFKIPNGTETLPLSTLYQPSGFVRE---PGVHIPT--EIQLQSQFINGLLQKSPANIPG---KHKPLLYQTYPKDPVMLENSGYGPFYMSTEYKTQY--DGKQCRDLGPGR-IGNLEMTGFTENNKTEEPLEGRPDACMCNHEAF-GNPPYNCRPMGHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLEWIRRNDPAEYINAINCNRIPTMMNAVYQGKQIQPIGNCDF--RTKTGNLEVTGHISNQDIFVQT---RDKPERFITNSMTQFYDPSLAGSERNGHINGQVQPTMENGFS-REVKLHKIEPQMDALTNSSK 1352
            +G V+ ++K SSGG +  LK Y T+   +YG   E F PR G+H G GY  N R  + Y  HLD +DNP IG+ I+   KS+T + Y+  ++P+G + LP +     S + RE   PG H     ++   +Q         P  I G   K  PL++Q   K     ENS YGP +M++EY ++Y  +     DL   + IG+ E TGFTE + T+ P+  +P +     +AF G+P  +    G SI  + Y P+  P G + LP +   +    GF++ N+       ++P RV   A               PA            +M +  YQ  Q     N     R   GN E TG   N   +V++   +D+ +R++T     +++    G +R G   G +QP     ++  E+  H +   MD+  N ++
Sbjct:    7 LGVVSPYVKMSSGGCSDPLKFYATSYCTAYGR--EEFKPRLGSHVGTGYKSNYRPLVSYQPHLDTLDNPAIGQQIRDTSKSVTSQSYSPLEVPDGKQPLPWNLHQTTSSYGREKLNPGPHSKEVRKVHFDTQ------DHGPQTITGLEPKEVPLIHQQQGKGSTEWENSHYGPRFMTSEYNSKYIKESPNHPDLLLKKTIGSKEETGFTEES-TKNPIVFQPPS-----QAFPGDPVLHP---GRSITKSDYLPVTHPQGSDFLPVLSRGSDRDTGFSRVNERT-----LNP-RVPTPAP-------------QPA------------SMSHRSYQPPQRMQQTNVALLGRESVGNKEPTGFTLNNPSYVRSSYEQDRDQRYLTTYNQGYFENIPKGLDREGWTRGGIQPQKAGAYALSELNNHTL---MDSTPNPTE 394

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BLAST of Retinal homeobox protein Rax vs. UniProt/SwissProt
Match: sp|Q2T9T0|PPR32_BOVIN (Protein phosphatase 1 regulatory subunit 32 OS=Bos taurus OX=9913 GN=PPP1R32 PE=2 SV=1)

HSP 1 Score: 124.405 bits (311), Expect = 1.054e-29
Identity = 112/419 (26.73%), Postives = 184/419 (43.91%), Query Frame = 3

Query:   90 IGPVTKHIKASSGGETYALKHYCTTNENSYGSGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPKIGEIIKTNYKSITQKDYNGFKIPNGTETLPLSTLYQPSGFVREPGVHIPTEIQLQSQFINGLLQKSPANIPG---KHKPLLYQTYPKDPVMLENSGYGPFYMSTEYKTQY---DGKQCRDLGPGRIGNLEMTGFTENNKTEEPLEGRPDACMCNHEAFGNPPYNCRPMGHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLEWIRRNDPAEYINAINCNRIPTMMNAVYQGKQIQPIGNCDFRTKTGNLEVTGHISNQDIFVQTRDKPE---RFITNSMTQFYDPSLAGSERNGHINGQVQPTMENGFSREVKLHKIE 1319
            +G V+ ++K SSGG T  LK Y T+   +YG   E F PR G+H G GY  N R  +FY  HLD +DN   GE    N++++T + Y   ++P+GT  LP +     SG+ RE    + T I+   +         P  I G   K  PLL+Q   K  +  EN+G+GP +M++EY ++Y      Q   L    +G  E TGFTE +  + P+  +P +     +    P       G S+  + + P+  P+G E LP +   +    GF++ N+     +         + S M   + +  +R                       Q   +  +G    R   GN E +G   N   +V++   P+   R++T     +++    G +R G   G +QP    G++    + ++E
Sbjct:    7 LGVVSPYVKMSSGGCTDPLKFYATSYCTAYGR--EDFKPRVGSHRGTGYKSNYRPVVFYQPHLDALDNTATGEQGCNNFQTVTSQSYRPLEVPDGTHPLPWNLHQTNSGYSREKASAV-TPIKEVRKVHFDTQDYGPQAITGLEPKDAPLLHQQQNKGSLEWENAGHGPRFMTSEYNSKYLKEPSHQPDLLQKNSVGAKEETGFTEESN-KNPIVFQPPSQALPGDPVLLP-------GRSVTKSDFLPISHPHGDEFLPVLARGSERETGFSRVNERT---LNPRVPPPCPEPSSMNHWQFQSPQR----------------------MQQTNVALLG----RETVGNKEPSGFSLNNPSYVRSPYDPDMDNRYLTTYNQGYFENIPKGLDREGWTRGGLQPQKPGGYALNQPVTRLE 385

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BLAST of Retinal homeobox protein Rax vs. UniProt/SwissProt
Match: sp|Q66HR9|PPR32_RAT (Protein phosphatase 1 regulatory subunit 32 OS=Rattus norvegicus OX=10116 GN=Ppp1r32 PE=2 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 1.793e-29
Identity = 115/412 (27.91%), Postives = 185/412 (44.90%), Query Frame = 3

Query:   90 IGPVTKHIKASSGGETYALKHYCTTNENSYGSGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPKIGEIIKTNYKSITQKDYNGFKIPNGTETLPLSTLYQPSGFVREPGVHIPTEIQLQSQFINGLLQKSPANIPG---KHKPLLYQTYPKDPVMLENSGYGPFYMSTEYKTQY--DGKQCRDLGPGR-IGNLEMTGFTENNKTEEPLEGRPDACMCNHEAFGNPPYNCRPMGHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLEWIRRNDPAEYINAINCNRIPTMMNAVYQGKQIQPIGNCDF--RTKTGNLEVTGHISNQDIFVQT---RDKPERFITNSMTQFYDPSLAGSERNGHINGQVQPTMENGFS 1292
            +G V+ ++K SSGG +  LK Y T+   +YG   E F PR G+H G GY  N R  + Y  HLD +DNP +GE I    KS+  + Y   ++P+G + LP +     SG+ RE     P   +++    +      P  I G   K  PL++Q   K     ENS YGP +M++EY ++Y  +     DL   + IG+ E TGFTE + T+ P+  +P +     +   +P       G SI  + Y P+  P G E LP +   +    G+++ ++       ++P R+   +S  T                         +M +  YQ  Q     N     R   GN E TG   N   +V++   +D+ +R++T     +++    G +R G   G +QP     ++
Sbjct:    7 LGVVSPYVKMSSGGCSDPLKFYATSYCTAYGR--EEFKPRMGSHVGTGYKSNYRPLVSYQPHLDTLDNPAVGEQIHDTSKSVASQSYAPLEVPDGKQPLPWNLHQTTSGYGREKVNIGPLSKEVRKVHFD-TQDHGPQTITGLEPKEVPLIHQQQGKGSTEWENSRYGPRFMTSEYNSKYLKESPNHPDLLLKKTIGSKEETGFTEES-TKNPIVFQPPSQAYPGDPVLHP-------GRSITKSDYLPISHPQGNEFLPVLARGSDRDTGYSRVSER-----SLNP-RMPTPSSQPT-------------------------SMSHRSYQPPQRMQQSNVALLGRESVGNKEPTGFTLNNPSYVRSSYEQDRDQRYLTTYNQGYFENIPKGLDREGWTRGGIQPQKAGAYA 376

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BLAST of Retinal homeobox protein Rax vs. UniProt/SwissProt
Match: sp|Q7Z5V6|PPR32_HUMAN (Protein phosphatase 1 regulatory subunit 32 OS=Homo sapiens OX=9606 GN=PPP1R32 PE=1 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 8.131e-25
Identity = 105/415 (25.30%), Postives = 183/415 (44.10%), Query Frame = 3

Query:   90 IGPVTKHIKASSGGETYALKHYCTTNENSYGSGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPKIGEIIKTNYKSITQKDYNGFKIPNGTETLPLSTLYQPSGFVRE-PGVHIPTEIQLQSQFINGLLQKSPANIPG---KHKPLLYQTYPKDPVMLENSGYGPFYMSTEYKTQYDGKQCRD-------LGPGRIGNLEMTGFTENNKTEEPLEGRPDACMCNHEAFGNPPYNCRPMGHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLEWIRRNDPAEYINAINCNRIPTMMNAVYQGKQIQPIGNCDFRTKTGNLEVTGHISNQDIFVQT---RDKPERFITNSMTQFYDPSLAGSERNGHINGQVQPTMENGFS 1292
            +G V+ ++K SSGG T  LK Y T+   +YG   E F PR G+H G GY  N +  +   + L+ +DNP  GE  + +++S+  + Y   ++P+G   LP S     SG+ RE P    PT+   +  F     +  P  I G   +  PLL+Q   +DP+  EN  +GP +M++EY ++Y     RD       L    IG  E +GFT+ +  + P+  +P +     +    P       G S+  + + P    +G E LP +   +     F++GN+ +   +         + S ++  + + + R                       Q   +  +G    R   G  E TG   N  ++V++    D+ +R++T     +++    G ++ G   G +QP M  G++
Sbjct:    7 LGVVSPYVKMSSGGYTDPLKFYATSYCTAYGR--EDFKPRVGSHVGTGYKSNFQPVVSCQASLEALDNPARGEQAQDHFQSVASQSYRPLEVPDGKHPLPWSMRQTSSGYGREKPSAGPPTKEVRKVHFDT--QEHGPQAITGLEPREVPLLHQQQGQDPLERENFRHGPRFMTSEYNSKY----LRDPLDQPDFLQKKSIGAKEGSGFTKQSH-QSPIVFQPPSQALPGDPALLP-------GQSVTKSDFLPKTHLHGDEFLPVLARGSKRETAFSRGNERI---LNPRVPPPCPEPSSVSHQQFQPLHR----------------------MQQTNVALLG----RETVGKKEPTGFSLNNPMYVRSPCDPDRDQRYLTTYNQGYFENIPKGLDQEGWTRGGIQPQMPGGYA 376

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BLAST of Retinal homeobox protein Rax vs. TrEMBL
Match: R7V6R9 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_180526 PE=4 SV=1)

HSP 1 Score: 277.715 bits (709), Expect = 2.238e-84
Identity = 165/422 (39.10%), Postives = 231/422 (54.74%), Query Frame = 3

Query:  108 HIKASSGGETYALKHYCTTNENSYGSGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPKIGEIIKTNYKSITQKDYNGFKIPNGTETLPLSTLYQPSGFVREPGVHIPTEIQLQSQFINGLLQKSPANIPGKHKPLLYQTYPKDPVMLENSGYGPFYMSTEYKTQYDGKQCR-DLGPGRIGNLEMTGFTENNKTEEPLEGRPDACMCNHEAFGNPPYNCRPMGHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLEWIRRNDPAEYINAINCNRIPTMMNAVYQGKQIQPIGNCDF--RTKTGNLEVTGHISNQDIFVQTRDKPERFITNSMTQFYDPSLAGSERNGHINGQVQPTMENGFSREVKLHKIEPQMDALTNSSKFTPY 1364
            HI+ S+G ++  +K Y T   ++Y   +  F P+ G H+G GY+ N R  ++Y   LD IDNP +G I++ NY +IT+  +  +   NG E LP S   + SGF+RE  + IPT    ++  I+  +  +PA I   HKPLL++   KDPV LENSGYGP YM+TE + ++ G Q   D+G   IG  E +GFT      EP+   P     +H+         RP G SI  T + P    +G +PLP I  R+   +GFT  NK  P+FV   P   YDKASD+  ARL+ I++ DPAEY N I+ N   ++  A ++G+Q       D   RT   + E TG+  N D FVQT D P RFIT+ MT+F D +  G +R GH  G VQP   +GF+R   +HK  P  D+ +      PY
Sbjct:   12 HIQKSNGADSDLMKFYTTQYSSTYSKKFSKFQPQQGHHTGTGYLSNFRPAVYYNEKLDQIDNPTMGAIVRDNYHTITENSFRPYTGLNGGEILPSSCKQKGSGFIREKALTIPTYCDARNVLIDTRVASAPAEIMPHHKPLLHKLKRKDPVELENSGYGPGYMTTETQERFRGAQPHLDVGRRSIGPKEGSGFTHAYNI-EPVTYHP---KYSHKGDIPAYLTDRPTGVSIMKTAFYPSEYSDGSDPLPRIAKRSDHESGFTNANKPKPEFVHRIPGDAYDKASDIPAARLDKIKKKDPAEYQNCIHPNNQSSVTKATFKGEQFPAQTEADRLGRTTVSHKEETGYTDNNDRFVQTADNPSRFITHYMTKFTDRTPQGIDRMGHTWGGVQPQKTDGFTRSTGVHKQGPSYDSTSIMRNLEPY 429

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BLAST of Retinal homeobox protein Rax vs. TrEMBL
Match: A0A1S3HJ15 (protein phosphatase 1 regulatory subunit 32 OS=Lingula unguis OX=7574 GN=LOC106155256 PE=4 SV=1)

HSP 1 Score: 259.61 bits (662), Expect = 2.265e-77
Identity = 156/430 (36.28%), Postives = 224/430 (52.09%), Query Frame = 3

Query:   93 GPVTKHIKASSGGETYALKHYCTTNENSYGSGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPKIGEIIKTNYKSITQKDYNGFKI-PNGTETLPLSTLYQPSGFVREPGVHIPTEIQLQSQFINGLLQKSPANIPGKHKPLLYQTYPKDPVMLENSGYGPFYMSTEYKTQYDGK--QCRDLGPGRIGNLEMTGFTENNKTEEPLEGRPDACMCNHEAFGNPPY-NCRPMGHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLEWIRRNDPAEYINAINCNRIPTMMNAVYQGKQIQPIGNCDF--RTKTGNLEVTGHISNQDIFVQTRDKPERFITNSMTQFYDPSLAGSERNGHINGQVQPTMENGFSREVKLHKIEPQMDALTNSSKFTPY 1364
            G  + HI+AS G ++  +K YCT + ++YG  WE F PR   H+G GY+ N R  ++Y+  LD +DNP +  +I  NY S+T+  +  +K+  +G + LP+S     SGF+R+  +  PT   +QS  I+  +  + A+I  ++KPLL++   KD V LENSGYGP +M+TE K ++ G+  +  D     +G  E TGFT      EP+   P +          P Y   RP G SI  TH++P    +G EPLP I   +    GFT G KA P FV       YDKA DM  A+LE  ++ DP EY+N  + N   ++    + G+Q       D    T  G  E+TG   N D FVQT D P RFIT+  T+F DP+    ER GH  G V     +GF++   +H   P ++         PY
Sbjct:   10 GSCSAHIQASKGADSNIMKFYCTEHASNYGKNWEKFRPRPSRHTGTGYLSNFRPGVYYSRRLDDVDNPAMARLIAKNYHSVTELAFQPYKVSSSGKDPLPMSVYQAGSGFIRQKPITAPTSRAVQSVCIDTRMSNASADILPRYKPLLHKIQSKDAVELENSGYGPGFMTTETKERFKGQPSERMDTTMKTLGPNEETGFTHAYNV-EPVTYHPGSPYKGDR----PGYITDRPTGRSIMKTHFQPSEFLHGGEPLPTIPIGSERGTGFTAGTKARPVFVNRVMGDAYDKADDMPRAKLERTKKQDPTEYMNMTHPNNYTSLAKDTFLGQQRPDKTEADRLETTGVGFKELTGFSENNDRFVQTSDNPRRFITHYATRFPDPTPEDKEREGHCRGGVHLQKSDGFTKSTAVHSYGPDINTTATLRSLHPY 434

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BLAST of Retinal homeobox protein Rax vs. TrEMBL
Match: A0A267GI60 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig019693g2 PE=4 SV=1)

HSP 1 Score: 252.292 bits (643), Expect = 8.650e-75
Identity = 148/412 (35.92%), Postives = 217/412 (52.67%), Query Frame = 3

Query:   90 IGPVTKHIKASSGGETYALKHYCTTNENSYGSGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPKIGEIIKTNYKSITQKDYNGFKIPNGTETLPLSTLYQPSGFVREPGVHIPTEIQLQSQFINGLLQKSPANIPGKHKPLLYQTYPKDPVMLENSGYGPFYMSTEYKTQYDGKQCR--DLGPGRIGNLEMTGFTENNKTEEPLEGRPDACMCNHEAFGNPPYNC-RPMGHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLEWIRRNDPAEYINAINCNRIPTMMNAVYQGKQI--QPIGNCDFRTKTGNLEVTGHISNQDIFVQTRDKPERFITNSMTQFYDPSLAGSERNGHINGQVQPTMENGFSREVKLH 1310
            +G    H+KAS GG+   +K YCTT+ +++GS + +F PR G H+G GY  N R  + Y+  LD +DNP IG I+  NY+++ Q+D+N +  P G+E LPL+    P+GFVR+  + +P + + Q                           P  P+    +  GP    +EY+ +Y G+Q    DL    +G  E +GFT N K  EP+     +   N +    P +   RP G SIYTT YR L +P+GREPLP++  R +  +GF+ G   +P F   +    Y K  D+ EARL  I ++DPAE++N    N   T+   +YQGKQ   Q   +    T  G+ E TGH++N D +VQ RD   RF+TN  TQF D +  G +R GH    V P  ++GF++  K H
Sbjct:   15 LGGANPHVKASRGGDANLMKFYCTTSASTHGSHFANFQPRPGRHTGTGYQANIRPTVQYSGRLDQVDNPAIGRIVAGNYRTVFQRDFNPYSTPTGSEPLPLTAHKVPTGFVRD-AMLMPMQRRRQG--------------------------PPQPLTRAATVAGP----SEYRDKYSGRQLAPVDLARSDVGPKERSGFTNNVKLNEPVRHTEHSPHRNEK----PGWMTHRPTGVSIYTTGYRSLEVPDGREPLPHLADRGSRDSGFSAGTVLLPPFAHRNMADAYSKLGDLPEARLRQISKSDPAEFVNVQQPNNHSTVYQNMYQGKQYPDQTYADRIGTTAVGDKEPTGHVTNDDGYVQKRDSAARFLTNYTTQFLDTTPVGKDREGHTAFGVLPAQDDGFTKSTKTH 391

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BLAST of Retinal homeobox protein Rax vs. TrEMBL
Match: A0A1I8H3Q4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 251.906 bits (642), Expect = 9.818e-75
Identity = 148/412 (35.92%), Postives = 217/412 (52.67%), Query Frame = 3

Query:   90 IGPVTKHIKASSGGETYALKHYCTTNENSYGSGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPKIGEIIKTNYKSITQKDYNGFKIPNGTETLPLSTLYQPSGFVREPGVHIPTEIQLQSQFINGLLQKSPANIPGKHKPLLYQTYPKDPVMLENSGYGPFYMSTEYKTQYDGKQCR--DLGPGRIGNLEMTGFTENNKTEEPLEGRPDACMCNHEAFGNPPYNC-RPMGHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLEWIRRNDPAEYINAINCNRIPTMMNAVYQGKQI--QPIGNCDFRTKTGNLEVTGHISNQDIFVQTRDKPERFITNSMTQFYDPSLAGSERNGHINGQVQPTMENGFSREVKLH 1310
            +G    H+KAS GG+   +K YCTT+ +++GS + +F PR G H+G GY  N R  + Y+  LD +DNP IG I+  NY+++ Q+D+N +  P G+E LPL+    P+GFVR+  + +P + + Q                           P  P+    +  GP    +EY+ +Y G+Q    DL    +G  E +GFT N K  EP+     +   N +    P +   RP G SIYTT YR L +P+GREPLP++  R +  +GF+ G   +P F   +    Y K  D+ EARL  I ++DPAE++N    N   T+   +YQGKQ   Q   +    T  G+ E TGH++N D +VQ RD   RF+TN  TQF D +  G +R GH    V P  ++GF++  K H
Sbjct:    8 LGGANPHVKASRGGDANLMKFYCTTSASTHGSHFANFQPRPGRHTGTGYQANIRPTVQYSGRLDQVDNPAIGRIVAGNYRTVFQRDFNPYSTPTGSEPLPLTAHKVPTGFVRD-AMLMPMQRRRQG--------------------------PPQPLTRAATVAGP----SEYRDKYSGRQLAPVDLARSDVGPKERSGFTNNVKLNEPVRHTEHSPHRNEK----PGWMTHRPTGVSIYTTGYRSLEVPDGREPLPHLADRGSRDSGFSAGTVLLPPFAHRNMADAYSKLGDLPEARLRQISKSDPAEFVNVQQPNNHSTVYQNMYQGKQYPDQTYADRIGTTAVGDKEPTGHVTNDDGYVQKRDSAARFLTNYTTQFLDTTPVGKDREGHTAFGVLPAQDDGFTKSTKTH 384

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BLAST of Retinal homeobox protein Rax vs. TrEMBL
Match: A0A1I8I616 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 1.241e-74
Identity = 148/412 (35.92%), Postives = 217/412 (52.67%), Query Frame = 3

Query:   90 IGPVTKHIKASSGGETYALKHYCTTNENSYGSGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPKIGEIIKTNYKSITQKDYNGFKIPNGTETLPLSTLYQPSGFVREPGVHIPTEIQLQSQFINGLLQKSPANIPGKHKPLLYQTYPKDPVMLENSGYGPFYMSTEYKTQYDGKQCR--DLGPGRIGNLEMTGFTENNKTEEPLEGRPDACMCNHEAFGNPPYNC-RPMGHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLEWIRRNDPAEYINAINCNRIPTMMNAVYQGKQI--QPIGNCDFRTKTGNLEVTGHISNQDIFVQTRDKPERFITNSMTQFYDPSLAGSERNGHINGQVQPTMENGFSREVKLH 1310
            +G    H+KAS GG+   +K YCTT+ +++GS + +F PR G H+G GY  N R  + Y+  LD +DNP IG I+  NY+++ Q+D+N +  P G+E LPL+    P+GFVR+  + +P + + Q                           P  P+    +  GP    +EY+ +Y G+Q    DL    +G  E +GFT N K  EP+     +   N +    P +   RP G SIYTT YR L +P+GREPLP++  R +  +GF+ G   +P F   +    Y K  D+ EARL  I ++DPAE++N    N   T+   +YQGKQ   Q   +    T  G+ E TGH++N D +VQ RD   RF+TN  TQF D +  G +R GH    V P  ++GF++  K H
Sbjct:    8 LGGANPHVKASRGGDANLMKFYCTTSASTHGSHFANFQPRPGRHTGTGYQANIRPTVQYSGRLDQVDNPAIGRIVAGNYRTVFQRDFNPYSTPTGSEPLPLTAHKVPTGFVRD-AMLMPMQRRRQG--------------------------PPQPLTRAATVAGP----SEYRDKYSGRQLAPVDLARSDVGPKERSGFTNNVKLNEPVRHTEHSPHRNEK----PGWMTHRPTGVSIYTTGYRSLEVPDGREPLPHLADRGSRDSGFSAGTVLLPPFAHRNMADAYSKLGDLPEARLRQISKSDPAEFVNVQQPNNHSTVYQNMYQGKQYPDQTYADRIGTTAVGDKEPTGHVTNDDGYVQKRDSAARFLTNYTTQFLDTTPVGKDREGHTAFGVLPAQDDGFTKSTKTH 384

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BLAST of Retinal homeobox protein Rax vs. Ensembl Nematostella
Match: EDO43736 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXX1])

HSP 1 Score: 197.978 bits (502), Expect = 8.642e-58
Identity = 138/436 (31.65%), Postives = 208/436 (47.71%), Query Frame = 3

Query:   93 GPVTKHIKASSGGETYALKHYCTTNENSYGSGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPKIGEIIKTNYKSITQKDYNGFKIPNGTETLPLST---LYQPSGFVRE-PGVHIPTEIQLQSQFINGLLQKSPANIPGKHKPLLYQTYPKDPVMLENSGYGPFYMSTEYKTQYDG--KQCRDLGPGRIGNLEMTGFTENNKTEEPLEGRPDACMCNHEAFGNPPYNCRPMGHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLEWIRRNDPAEYINAINCNRIPTMMNAVYQGKQIQPIGNCDF--RTKTGNLEVTGHISNQDIF---VQTRDKPERFITNSMTQFYDPSLAGSERNGHINGQVQPTMENGFSREVKLHKIEPQMDALTNSSKFTPYQ 1367
            GP   H+  S G +   +K YCT     YG   + F PR G H G GY  N R  ++Y   LD +DNP +G ++  NY SIT+K +   K   G +  P S    +   SGFV++ PG  +P   Q+ +  ++         +   H+P+L+    KDP+  EN+ +GP YMSTE  T + G   Q  D     +G  E +GFT +   +EP+   P   M  +E   +P +  RP G SI    +RP+   +G E LP +   +  + GFT G KA P F     D  Y K ++      E +++ DP E+ N  N +   +M    ++GKQ Q +        T  GN E+TG+  N D F    +T D   RF T+   +FYD +  G  R G   G+V   + +GF++   +H+  P ++      +  PYQ
Sbjct:    7 GPGNAHMLQSKGADINVMKFYCTEYATRYGE--DGFNPRTGKHLGTGYQSNFRPGVYYNRRLDELDNPAMGSLVADNYASITKKHFLPSKGSPGND--PFSRGMFMTATSGFVKDIPGT-LPRSKQVAAVHVD---TSRAGTVYPFHRPILHSLSQKDPISRENARHGPLYMSTEAHTNFKGIPSQRMDTSKKTVGYKEGSGFT-HAYNDEPITFYP---MEAYEGLRDPRFTHRPTGFSIMKGSFRPVEYQHGNEQLPVLSHGSERNTGFTHGTKARPVFYHHTMDEAYTKLNETHPRVQERVQKRDPCEFSNMTNPHNHTSMAKLTFRGKQRQDLSEAGRLGNTAVGNKELTGYSENNDQFFETAETADTLRRFGTHYRAKFYDMNPMGEARMGRTRGEVMVQLPDGFTKSTSVHEFGPVINTTVQLRELEPYQ 430

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BLAST of Retinal homeobox protein Rax vs. Planmine SMEST
Match: SMESG000019089.1 (SMESG000019089.1)

HSP 1 Score: 900.582 bits (2326), Expect = 0.000e+0
Identity = 443/448 (98.88%), Postives = 447/448 (99.78%), Query Frame = 3

Query:   81 MSGIGPVTKHIKASSGGETYALKHYCTTNENSYGSGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPKIGEIIKTNYKSITQKDYNGFKIPNGTETLPLSTLYQPSGFVREPGVHIPTEIQLQSQFINGLLQKSPANIPGKHKPLLYQTYPKDPVMLENSGYGPFYMSTEYKTQYDGKQCRDLGPGRIGNLEMTGFTENNKTEEPLEGRPDACMCNHEAFGNPPYNCRPMGHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLEWIRRNDPAEYINAINCNRIPTMMNAVYQGKQIQPIGNCDFRTKTGNLEVTGHISNQDIFVQTRDKPERFITNSMTQFYDPSLAGSERNGHINGQVQPTMENGFSREVKLHKIEPQMDALTNSSKFTPYQXXXXXXXXXXXXXPTKMPA 1424
            MSGIGP++KHIKASSGGETYALKHYCTTNENSYGSGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPK+GEIIKTNYKSITQKDYNGFKIPNGTETLPLSTLYQPSGFVREPGVHIPTEIQLQSQFINGLLQKSPANIPGKHKPLLYQTYPKDPVMLENSGYGPFYMSTEYKTQYDGKQCRDLGPGRIGNLEMTGFTENNKTEEPLEGRPDACMCNHEAFGNPPYNCRP GHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLEWIRRNDPAEYINAINCNRIPTMMNAVYQGKQIQPIGNCDFRTKTGNLEVTGHISNQDIFVQTRDKPERFITNSMTQFYDPSLAGSERNGHINGQVQPTMENGF+REVKLHKIEPQMDALTNSSKFTPYQSRSIIARESISISPTKMPA
Sbjct:    1 MSGIGPISKHIKASSGGETYALKHYCTTNENSYGSGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPKMGEIIKTNYKSITQKDYNGFKIPNGTETLPLSTLYQPSGFVREPGVHIPTEIQLQSQFINGLLQKSPANIPGKHKPLLYQTYPKDPVMLENSGYGPFYMSTEYKTQYDGKQCRDLGPGRIGNLEMTGFTENNKTEEPLEGRPDACMCNHEAFGNPPYNCRPTGHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLEWIRRNDPAEYINAINCNRIPTMMNAVYQGKQIQPIGNCDFRTKTGNLEVTGHISNQDIFVQTRDKPERFITNSMTQFYDPSLAGSERNGHINGQVQPTMENGFTREVKLHKIEPQMDALTNSSKFTPYQSRSIIARESISISPTKMPA 448

[Close]

BLAST of Retinal homeobox protein Rax vs. Planmine SMEST
Match: SMESG000029217.1 (SMESG000029217.1)

HSP 1 Score: 171.785 bits (434), Expect = 3.210e-48
Identity = 131/425 (30.82%), Postives = 190/425 (44.71%), Query Frame = 3

Query:   78 LMSGIGPVTKHIKASSGGETYALKHYCTTNENSYGSGWEHFTPRCGAHSGAGYMPNNRGQIFYTSHLDVIDNPKIGEIIKTNYKSITQKDYNGFKIPNGTETLPLSTLYQPSGFVREPGVHIPTEIQLQSQFINGLLQKSPANIPGKHKPLLYQTYP-KDPVMLENSGYGPFYMSTEYKTQYDGKQC--RDLGPGRIGNLEMTGFTENNKTEEPLEGRPDACMCNHEAFGNPPYNCRPMGHSIYTTHYRPLGIPNGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLEWIRRNDPAEYINAINCNRIPTMMNAVYQGKQIQPIGNCDF--RTKTGNLEVTGHISNQDIFVQTRDKPERFITNSMTQFYDPSLAGSERNGHINGQVQPTMENGFSREVKLHKIEPQMDAL 1337
            + S  GPV+ HIK S GG +    HY TT    YG  W +         G  YM + R  I Y+S LD  DNP +  II TNY S+T++D                                         F+   L     N+   HKP++   Y     + +  S + P   +T YKT ++ K     D     IG  E++GF+ N+   + L  RP +   N           RP G ++Y+T YR   IP+G EPLP I   +  S+GF KGN+  PDF  I P +   K    +   L  +++ DPAEY+N       P++ +  +     QP    +   R K G+ E TG ++N+  +VQT+D PERFIT++MT++Y+P L G E NGH+   V     +GF +   +   E     L
Sbjct:    4 IYSQNGPVSIHIKESLGGSSNPFNHYSTTYNLKYGKHWVNREMYREPQPGGNYMWSKRCHIGYSSSLDDFDNPFLKPIIMTNYLSVTKRD-----------------------------------------FVKPKLTHREYNLSNIHKPIVPAGYEFHTELTIPKSMHNPNDWTTTYKTLHNEKLPIPVDFHRDPIGKKELSGFSLNSPIIDTLSFRPHSTYKNDWPRTE---TNRPTGQTVYSTDYRKYSIPDGTEPLPEIPFGSDRSSGFVKGNQQRPDF--IPPSQEVIKPKYYSPQELAKLKKTDPAEYLNNAYPYPQPSVTHGTFTDPFSQPPTEEEVLGRVKVGDKERTGFVANEINYVQTKDNPERFITHNMTKYYNPMLIGCEVNGHVAFNVLDIESDGFIKNNGISNFEKSSKML 382

The following BLAST results are available for this feature:

BLAST of Retinal homeobox protein Rax vs. Ensembl Human
BLAST of Retinal homeobox protein Rax vs. Ensembl Celegans
BLAST of Retinal homeobox protein Rax vs. Ensembl Fly
BLAST of Retinal homeobox protein Rax vs. Ensembl Zebrafish
BLAST of Retinal homeobox protein Rax vs. Ensembl Xenopus
BLAST of Retinal homeobox protein Rax vs. Ensembl Mouse
BLAST of Retinal homeobox protein Rax vs. UniProt/SwissProt
BLAST of Retinal homeobox protein Rax vs. TrEMBL
BLAST of Retinal homeobox protein Rax vs. Ensembl Cavefish
BLAST of Retinal homeobox protein Rax vs. Ensembl Sea Lamprey
BLAST of Retinal homeobox protein Rax vs. Ensembl Yeast
BLAST of Retinal homeobox protein Rax vs. Ensembl Nematostella
BLAST of Retinal homeobox protein Rax vs. Ensembl Medaka
BLAST of Retinal homeobox protein Rax vs. Planmine SMEST

BLAST of Retinal homeobox protein Rax vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2


Match Name	E-value	Identity	Description
PPP1R32	1.693e-25	25.30	protein phosphatase 1 regulatory subunit 32 [Sourc...	[more]
PPP1R32	6.827e-23	25.30	protein phosphatase 1 regulatory subunit 32 [Sourc...	[more]

BLAST of Retinal homeobox protein Rax vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Retinal homeobox protein Rax vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Retinal homeobox protein Rax vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ppp1r32	2.834e-11	27.41	protein phosphatase 1, regulatory subunit 32 [Sour...	[more]

BLAST of Retinal homeobox protein Rax vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1


Match Name	E-value	Identity	Description
rnf11	4.891e-30	28.54	ring finger protein 11 [Source:Xenbase;Acc:XB-GENE...	[more]

BLAST of Retinal homeobox protein Rax vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2


Match Name	E-value	Identity	Description
Ppp1r32	1.447e-31	29.13	protein phosphatase 1, regulatory subunit 32 [Sour...	[more]
Ppp1r32	1.447e-31	29.13	protein phosphatase 1, regulatory subunit 32 [Sour...	[more]

BLAST of Retinal homeobox protein Rax vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4


Match Name	E-value	Identity	Description
sp\|Q148A4\|PPR32_MOUSE	9.003e-31	29.38	Protein phosphatase 1 regulatory subunit 32 OS=Mus...	[more]
sp\|Q2T9T0\|PPR32_BOVIN	1.054e-29	26.73	Protein phosphatase 1 regulatory subunit 32 OS=Bos...	[more]
sp\|Q66HR9\|PPR32_RAT	1.793e-29	27.91	Protein phosphatase 1 regulatory subunit 32 OS=Rat...	[more]
sp\|Q7Z5V6\|PPR32_HUMAN	8.131e-25	25.30	Protein phosphatase 1 regulatory subunit 32 OS=Hom...	[more]

BLAST of Retinal homeobox protein Rax vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
R7V6R9	2.238e-84	39.10	Uncharacterized protein OS=Capitella teleta OX=283...	[more]
A0A1S3HJ15	2.265e-77	36.28	protein phosphatase 1 regulatory subunit 32 OS=Lin...	[more]
A0A267GI60	8.650e-75	35.92	Uncharacterized protein (Fragment) OS=Macrostomum ...	[more]
A0A1I8H3Q4	9.818e-75	35.92	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]
A0A1I8I616	1.241e-74	35.92	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]

BLAST of Retinal homeobox protein Rax vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Retinal homeobox protein Rax vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Retinal homeobox protein Rax vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Retinal homeobox protein Rax vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO43736	8.642e-58	31.65	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Retinal homeobox protein Rax vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Retinal homeobox protein Rax vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2


Match Name	E-value	Identity	Description
SMESG000019089.1	0.000e+0	98.88	SMESG000019089.1	[more]
SMESG000029217.1	3.210e-48	30.82	SMESG000029217.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30028057 ID=SMED30028057|Name=Retinal homeobox protein Rax|organism=Schmidtea mediterranea sexual|type=transcript|length=1510bp
CTAGTTATCTAGCAACTGAAAATATTCTTTTTCAAAGGTAATTTTCTTAA
ATAAAGATAAATCAAAACTCAGGAAACCTAATGTCTGGAATTGGTCCTGT
AACAAAACATATAAAAGCATCGAGTGGCGGGGAAACATATGCTTTGAAGC
ATTATTGCACAACTAACGAAAACTCCTATGGATCTGGCTGGGAACACTTT
ACACCGAGATGTGGAGCTCATTCGGGAGCTGGTTACATGCCTAATAATCG
AGGGCAAATTTTTTACACAAGTCATCTTGATGTCATTGATAATCCTAAAA
TAGGTGAAATAATTAAAACAAATTACAAATCGATTACTCAAAAAGACTAT
AATGGATTCAAAATTCCGAATGGAACAGAAACTCTACCTCTTTCGACTCT
CTACCAACCAAGTGGATTCGTTAGAGAACCTGGTGTTCACATTCCTACAG
AAATACAGTTGCAAAGTCAATTTATAAATGGGTTATTACAAAAATCTCCA
GCAAATATTCCTGGTAAGCATAAACCACTTTTATATCAAACATATCCCAA
GGATCCAGTGATGCTTGAAAATAGCGGCTACGGTCCGTTTTACATGTCTA
CTGAATATAAGACTCAATATGACGGAAAGCAATGCAGAGATTTGGGTCCA
GGAAGAATTGGAAATCTCGAAATGACAGGATTTACTGAAAATAATAAAAC
AGAGGAACCTTTAGAAGGTAGGCCAGACGCGTGCATGTGCAACCATGAAG
CGTTTGGAAATCCTCCATATAATTGCAGGCCAATGGGCCATAGTATATAT
ACAACACATTATCGTCCATTAGGTATACCAAATGGCAGAGAACCTCTTCC
TAATATTGAAACCAGAGCAGCGATTAGCAATGGATTTACAAAAGGAAATA
AAGCAGTTCCAGATTTTGTGGAAATTCATCCAGACAGAGTTTATGATAAA
GCAAGTGATATGACCGAGGCAAGATTGGAATGGATCAGAAGAAATGATCC
AGCTGAATATATTAATGCAATTAATTGTAACAGAATCCCCACAATGATGA
ATGCTGTATATCAAGGCAAACAAATTCAACCGATAGGAAATTGTGATTTC
AGAACCAAAACTGGTAACCTTGAAGTTACAGGCCATATAAGCAATCAAGA
TATATTTGTACAAACGAGAGATAAACCCGAAAGATTCATCACCAATAGTA
TGACACAGTTTTATGATCCATCTTTGGCTGGGAGCGAAAGAAATGGGCAT
ATAAATGGTCAAGTTCAACCAACCATGGAGAATGGGTTTAGTAGAGAAGT
AAAATTGCATAAAATTGAACCACAAATGGACGCTTTAACAAATTCTTCAA
AATTTACTCCATATCAGTCGAGGTCAATAATTGCAAGGGAGTCCATTTCT
ATTTCTCCAACAAAAATGCCAGCTTGAAAATCATAAATTAGTTTAAAATA
TAGAACAAAAAATTTTATTTTGTTTTCCAAATAATCAAAATTTTAAAATT
CTTCAAAAAA

protein sequence of SMED30028057-orf-1

>SMED30028057-orf-1 ID=SMED30028057-orf-1|Name=SMED30028057-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=449bp
MSGIGPVTKHIKASSGGETYALKHYCTTNENSYGSGWEHFTPRCGAHSGA
GYMPNNRGQIFYTSHLDVIDNPKIGEIIKTNYKSITQKDYNGFKIPNGTE
TLPLSTLYQPSGFVREPGVHIPTEIQLQSQFINGLLQKSPANIPGKHKPL
LYQTYPKDPVMLENSGYGPFYMSTEYKTQYDGKQCRDLGPGRIGNLEMTG
FTENNKTEEPLEGRPDACMCNHEAFGNPPYNCRPMGHSIYTTHYRPLGIP
NGREPLPNIETRAAISNGFTKGNKAVPDFVEIHPDRVYDKASDMTEARLE
WIRRNDPAEYINAINCNRIPTMMNAVYQGKQIQPIGNCDFRTKTGNLEVT
GHISNQDIFVQTRDKPERFITNSMTQFYDPSLAGSERNGHINGQVQPTME
NGFSREVKLHKIEPQMDALTNSSKFTPYQSRSIIARESISISPTKMPA*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **molecular function**
Term	Definition
GO:0003677	DNA binding
GO:0003700	transcription factor activity, sequence-specific DNA binding
GO:0043565	sequence-specific DNA binding
GO:0019902	phosphatase binding

Vocabulary: **cellular component**
Term	Definition
GO:0005634	nucleus
GO:0005575	cellular_component

Vocabulary: **biological process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated
GO:0006366	transcription from RNA polymerase II promoter
GO:0008150	biological_process

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000034	epidermis
PLANA:0000099	neuron
PLANA:0000418	head
PLANA:0002032	epidermal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR031410	PPP1R32

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR031410	Protein phosphatase 1 regulatory subunit 32	PFAM	PF15691	PPP1R32	coord: 4..437 e-value: 1.1E-91 score: 308.0
IPR031410	Protein phosphatase 1 regulatory subunit 32	PANTHER	PTHR34349	FAMILY NOT NAMED	coord: 4..437

Main menu

Retinal homeobox protein Rax

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 12

Owlery

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