Orphan G-protein coupled receptor 22
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30027972 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Human
Match: QRFPR (pyroglutamylated RFamide peptide receptor [Source:HGNC Symbol;Acc:HGNC:15565]) HSP 1 Score: 119.398 bits (298), Expect = 1.741e-29 Identity = 90/328 (27.44%), Postives = 164/328 (50.00%), Query Frame = 1
Query: 67 RWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISR-------LLVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATE---VLPYTLLLGYLHSSMSPIVYWFINSSFKQSLI 1020
R +L + LIF L++ GN LV + + K MR NI + +L+L+DL +T CIP + ++I W+ G +CK+ + Q + + L++ +++ R + Y N+R + +L +VW+++ + P+ + ++ ++L E C E+W +Q IY +L I F+LPL VM+I YS K+ + I+ V D + + KE S A R+KK V M++ ++ +FA+CW PF + +MI + +F+ Y + ++G+ +S +PIVY F+N +FK++++
Sbjct: 43 RAKLALVLTGVLIFALALFGNALVFYVVTRSKAMRTVTNIFICSLALSDLLITFFCIPVTMLQNISDNWLGGAFICKMVPFVQSTAVVTEILTMTCIAVERHQGLVHPFKMKWQYTNRRAFT----MLGVVWLVAVIVGSPMWHVQQLEIKYDFL-----YEKEHICCLEEWTSPVHQKIYTTFILVILFLLPLMVMLILYS----KIGYELWIKKRVGDGSVLRTIH--------GKEMSKIA-----RKKKRAVIMMVTVVALFAVCWAPFHVVHMMIEYSNFEKEYDDVTIKMIFAIVQIIGFSNSICNPIVYAFMNENFKKNVL 344
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Human
Match: HCRTR2 (hypocretin receptor 2 [Source:HGNC Symbol;Acc:HGNC:4849]) HSP 1 Score: 106.686 bits (265), Expect = 5.064e-25 Identity = 95/348 (27.30%), Postives = 169/348 (48.56%), Query Frame = 1
Query: 28 LDIIYSQYFQPLTRWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL------LVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDD----QDHTKVLGYLR-----NHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQ-LNSLMILFMSFDNAYKATEV---LPYTLLLGYLHSSMSPIVYWFINSSFKQS 1014
L ++ +Y P + V I ++F++++IGN LV + K MR N ++NLSLAD+ VT+ C+PA L I W G+ +CK+ Y Q V ++ S L+L +++ R L+ KS + NS ++++WI+S + IP + + + L L T C E+W Y +Y+I + ++ PL +M++ Y ++ R + R I Q K L + S+ +++ A ++ R ++ ML+I++LVFAIC++P LN L +F F + V ++ L Y +S+ +PI+Y F++ F++
Sbjct: 40 LRYLWREYLHP-KEYEWVLIAGYIIVFVVALIGNVLVCVAVWKNHHMRTVTNYFIVNLSLADVLVTITCLPATLVVDITETWFFGQSLCKVIPYLQTVSVSVSVLTLSCIALDRWYAICHPLMFKSTAKRARNS-----IVIIWIVSCIIMIPQAIVMECSTVFPGLANKTTLFT---VCDERWGGEIYPKMYHICFFLVTYMAPLCLMVLAY----LQIFRKLWCRQIPGTSSVVQRKWKPLQPVSQPRGPGQPTKSRMSAVAAEIKQIRARRKTARMLMIVLLVFAICYLPISILNVLKRVFGMFAHTEDRETVYAWFTFSHWLVYANSAANPIIYNFLSGKFREE 374
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Human
Match: HCRTR2 (hypocretin receptor 2 [Source:HGNC Symbol;Acc:HGNC:4849]) HSP 1 Score: 106.686 bits (265), Expect = 5.064e-25 Identity = 95/348 (27.30%), Postives = 169/348 (48.56%), Query Frame = 1
Query: 28 LDIIYSQYFQPLTRWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL------LVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDD----QDHTKVLGYLR-----NHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQ-LNSLMILFMSFDNAYKATEV---LPYTLLLGYLHSSMSPIVYWFINSSFKQS 1014
L ++ +Y P + V I ++F++++IGN LV + K MR N ++NLSLAD+ VT+ C+PA L I W G+ +CK+ Y Q V ++ S L+L +++ R L+ KS + NS ++++WI+S + IP + + + L L T C E+W Y +Y+I + ++ PL +M++ Y ++ R + R I Q K L + S+ +++ A ++ R ++ ML+I++LVFAIC++P LN L +F F + V ++ L Y +S+ +PI+Y F++ F++
Sbjct: 40 LRYLWREYLHP-KEYEWVLIAGYIIVFVVALIGNVLVCVAVWKNHHMRTVTNYFIVNLSLADVLVTITCLPATLVVDITETWFFGQSLCKVIPYLQTVSVSVSVLTLSCIALDRWYAICHPLMFKSTAKRARNS-----IVIIWIVSCIIMIPQAIVMECSTVFPGLANKTTLFT---VCDERWGGEIYPKMYHICFFLVTYMAPLCLMVLAY----LQIFRKLWCRQIPGTSSVVQRKWKPLQPVSQPRGPGQPTKSRMSAVAAEIKQIRARRKTARMLMIVLLVFAICYLPISILNVLKRVFGMFAHTEDRETVYAWFTFSHWLVYANSAANPIIYNFLSGKFREE 374
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Human
Match: NPY1R (neuropeptide Y receptor Y1 [Source:HGNC Symbol;Acc:HGNC:7956]) HSP 1 Score: 98.5969 bits (244), Expect = 2.013e-22 Identity = 82/313 (26.20%), Postives = 154/313 (49.20%), Query Frame = 1
Query: 103 IFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKSYQNKRVNS--GFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATEVLPYTLLLGYLH------SSMSPIVYWFINSSFKQSL 1017
+ +L V GN ++I+ LK K MR NI ++NLS +DL V ++C+P ++ +W+ GE MCK++ + Q V IT S SL +++ R + + + R N+ + G+ + +W+++ +++P L ++ + N++L CF+++ ++ Y +L + + PL + I Y FK I IRL + ++ +R++ S ET K + ML+ +++ FA+CW+P L I FD ++ + LL H + ++PI Y F+N +F++ L
Sbjct: 50 VIILGVSGNLALIIIILKQKEMRNVTNILIVNLSFSDLLVAIMCLPFTFVYTLMDHWVFGEAMCKLNPFVQCVSITVSIFSLVLIAVERHQLIINPRGWRPNNRHAYVGIAV-IWVLAVASSLPFLIYQVMTD-EPFQNVTLDAYKDKYVCFDQFPSDSHRLSYTTLLLVLQYFGPLCFIFICY----FK----IYIRL----KRRNNMMDKMRDNKYRSSET------------KRINIMLLSIVVAFAVCWLP-----LTIFNTVFDWNHQIIATCNHNLLFLLCHLTAMISTCVNPIFYGFLNKNFQRDL 331
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Human
Match: NPFFR2 (neuropeptide FF receptor 2 [Source:HGNC Symbol;Acc:HGNC:4525]) HSP 1 Score: 98.9821 bits (245), Expect = 2.473e-22 Identity = 84/339 (24.78%), Postives = 165/339 (48.67%), Query Frame = 1
Query: 28 LDIIYSQYFQPLTRWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL-LVTKSYQNK-RVNSGFPGLLILVWIISTVATIP---VLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATEVL-----PYTLLLGYLHSSMSPIVYWFINSSFKQS 1014
++I Y Y+ + ++I LIF L ++GN +V + ++ K M N+ +LNL+++DL V + C+P L +I W G MCKIS QG+ + +S +L +++ R V ++ K + + F +++++W+++ P +L+ ++ LN S + + +C E W E + IY + A ++ PL +++I Y I + R +Q+ V+ R+K+ ++ ML+I+ L+F + W+P L +LM+L D + +++ P+ L + +SS++PI+Y F N +F++
Sbjct: 30 INITYVNYYLHQPQVAAIFIISYFLIFFLCMMGNTVVCFIVMRNKHMHTVTNLFILNLAISDLLVGIFCMPITLLDNIIAGWPFGNTMCKISGLVQGISVAASVFTLVAIAVDRFQCVVYPFKPKLTIKTAF-VIIMIIWVLAITIMSPSAVMLHVQEEKYYRVRLN-SQNKTSPVYWCREDWPNQEMRKIYTTVLFANIYLAPLSLIVIMYGRIGISLFRAAVPHTGRKNQEQWHVVS---------------------RKKQKIIKMLLIVALLFILSWLP--LWTLMMLSDYADLSPNELQIINIYIYPFAHWLAFGNSSVNPIIYGFFNENFRRG 343
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Celegans
Match: ckr-2 (CholecystoKinin Receptor homolog [Source:UniProtKB/TrEMBL;Acc:G4SNP8]) HSP 1 Score: 93.2041 bits (230), Expect = 6.823e-21 Identity = 86/361 (23.82%), Postives = 168/361 (46.54%), Query Frame = 1
Query: 100 LIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISR-LLVTKSYQNK--RVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRI-----------------------------------RLIVDDQDHT---KVLGYLRNHSNLSKETS--LNAHSREYR-QKKFMVGMLIILILVFAICWMPFQLNSLMIL----FMSFDNAYKATEVLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLLGQQ 1038
+IFLLSV+GN +V+I+ +K + MR NI L+NL+ +D+ +++VC+P L + W+ G MCKI Y Q V++T+S+ +L ++ R + K ++ + S ++ LVW+I+ A I +L+ + P+ I Y F Y + M + ++PL VM Y ++ + I++ +L QD T G+ ++ +++ E + H+++ K+ ++ MLI+++++F CW P + L+++ F S + + +++ + LL Y+ S +PI Y F+N F+ ++ G++
Sbjct: 27 MIFLLSVVGNSVVLIVIIKQRAMRSITNIYLMNLAASDMMLSVVCMPPTLVSMVMNCWMFGNYMCKILAYLQPVVVTASAYTLAVIAFERYFAICKPLHSRIWQTRSHAYAMITLVWVIAIAANILMLFMYEQQTYSSNGYTCAPIHPPI-----------YHFAYQVYMTVVLLVIPLVVMAGLYGNVITSLKSGIKLEIASVDPPLATATTTAIVASMTDEQKLSFWNKLSNKLTFSQQDKTVQHPNFGHRKSDTSICLENPSLRSTHTQKSAMAKQRVIKMLIVVVIIFFCCWTPSYIWWLLLIAGDSFQSLNLSVWNSDINTFITLLTYISSCTNPITYCFLNKKFRNAVYATFGRK 376
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Celegans
Match: npr-2 (NeuroPeptide Receptor family [Source:UniProtKB/TrEMBL;Acc:A0A061AKJ8]) HSP 1 Score: 93.2041 bits (230), Expect = 7.593e-21 Identity = 80/329 (24.32%), Postives = 151/329 (45.90%), Query Frame = 1
Query: 103 IFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL-LVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQ-----DHTKVLGYLRNHSNLSKETSLNAHSREYR-----QKKFMVGMLIILILVFAICWMPFQLNSLMI-----LFMSFDNAYKATE----VLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLL 1029
IFLL ++GN V+ L +K ++++ QNI +LNL +++ + L +P ++Y W +CK+ QG I S+ SL +++ R LV + ++ K + + +L+W+IS V +P ++ +L YC E W E + Y +L F+ P M Y I ++ + + +L + +++ G + +++ E N + R Q++ +L ++L+FA W+P + +LMI F S + + +T+ V L+ L + +P +Y ++N FK+ LIK L
Sbjct: 36 IFLLGILGNSAVLYLTMKHRQLQTVQNIFILNLCASNVLMCLTSLPITFITNVYKQWFFSSPVCKLIPLVQGASIFVSTFSLSAIALDRYNLVVRPHKQKLSSRSAMMIALLIWVISVVVCMPYGWYMDVEKLNGLCG---------EYCSEHWPLAEVRKGYTFLVLITQFLFPFATMAFCYYNIFSRLRQRVETKLKKLSERSQLLENSTTCGTTNHIVSINAEAVQNGLENKQRLAVLAQQRRTTTILSCMVLLFAFTWLPHNVVTLMIEYDGFFFHSDETSATSTDHTYIVSMTAHLISMLTNVTNPFLYAWLNPMFKEMLIKTL 355
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Celegans
Match: ckr-2 (CholecystoKinin Receptor homolog [Source:UniProtKB/TrEMBL;Acc:G4SNP8]) HSP 1 Score: 93.2041 bits (230), Expect = 1.153e-20 Identity = 89/378 (23.54%), Postives = 177/378 (46.83%), Query Frame = 1
Query: 100 LIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISR-LLVTKSYQNK--RVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRI-----------------------------------RLIVDDQDHT---KVLGYLRNHSNLSKETS--LNAHSREYR-QKKFMVGMLIILILVFAICWMPFQLNSLMIL----FMSFDNAYKATEVLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLLGQQ*EIHNANYQSLFSIIFN 1089
+IFLLSV+GN +V+I+ +K + MR NI L+NL+ +D+ +++VC+P L + W+ G MCKI Y Q V++T+S+ +L ++ R + K ++ + S ++ LVW+I+ A I +L+ + P+ I Y F Y + M + ++PL VM Y ++ + I++ +L QD T G+ ++ +++ E + H+++ K+ ++ MLI+++++F CW P + L+++ F S + + +++ + LL Y+ S +PI Y F+N F+ ++ G++ + + ++Q ++ IF
Sbjct: 27 MIFLLSVVGNSVVLIVIIKQRAMRSITNIYLMNLAASDMMLSVVCMPPTLVSMVMNCWMFGNYMCKILAYLQPVVVTASAYTLAVIAFERYFAICKPLHSRIWQTRSHAYAMITLVWVIAIAANILMLFMYEQQTYSSNGYTCAPIHPPI-----------YHFAYQVYMTVVLLVIPLVVMAGLYGNVITSLKSGIKLEIASVDPPLATATTTAIVASMTDEQKLSFWNKLSNKLTFSQQDKTVQHPNFGHRKSDTSICLENPSLRSTHTQKSAMAKQRVIKMLIVVVIIFFCCWTPSYIWWLLLIAGDSFQSLNLSVWNSDINTFITLLTYISSCTNPITYCFLNKKFRNAVYATFGRKKNMRH-HFQKVYFDIFE 392
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Celegans
Match: npr-2 (NeuroPeptide Receptor family [Source:UniProtKB/TrEMBL;Acc:A0A061AKJ8]) HSP 1 Score: 92.8189 bits (229), Expect = 1.278e-20 Identity = 80/329 (24.32%), Postives = 151/329 (45.90%), Query Frame = 1
Query: 103 IFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL-LVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQ-----DHTKVLGYLRNHSNLSKETSLNAHSREYR-----QKKFMVGMLIILILVFAICWMPFQLNSLMI-----LFMSFDNAYKATE----VLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLL 1029
IFLL ++GN V+ L +K ++++ QNI +LNL +++ + L +P ++Y W +CK+ QG I S+ SL +++ R LV + ++ K + + +L+W+IS V +P ++ +L YC E W E + Y +L F+ P M Y I ++ + + +L + +++ G + +++ E N + R Q++ +L ++L+FA W+P + +LMI F S + + +T+ V L+ L + +P +Y ++N FK+ LIK L
Sbjct: 61 IFLLGILGNSAVLYLTMKHRQLQTVQNIFILNLCASNVLMCLTSLPITFITNVYKQWFFSSPVCKLIPLVQGASIFVSTFSLSAIALDRYNLVVRPHKQKLSSRSAMMIALLIWVISVVVCMPYGWYMDVEKLNGLCG---------EYCSEHWPLAEVRKGYTFLVLITQFLFPFATMAFCYYNIFSRLRQRVETKLKKLSERSQLLENSTTCGTTNHIVSINAEAVQNGLENKQRLAVLAQQRRTTTILSCMVLLFAFTWLPHNVVTLMIEYDGFFFHSDETSATSTDHTYIVSMTAHLISMLTNVTNPFLYAWLNPMFKEMLIKTL 380
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Celegans
Match: npr-2 (NeuroPeptide Receptor family [Source:UniProtKB/TrEMBL;Acc:A0A061AKJ8]) HSP 1 Score: 92.4337 bits (228), Expect = 1.547e-20 Identity = 80/329 (24.32%), Postives = 151/329 (45.90%), Query Frame = 1
Query: 103 IFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL-LVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQ-----DHTKVLGYLRNHSNLSKETSLNAHSREYR-----QKKFMVGMLIILILVFAICWMPFQLNSLMI-----LFMSFDNAYKATE----VLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLL 1029
IFLL ++GN V+ L +K ++++ QNI +LNL +++ + L +P ++Y W +CK+ QG I S+ SL +++ R LV + ++ K + + +L+W+IS V +P ++ +L YC E W E + Y +L F+ P M Y I ++ + + +L + +++ G + +++ E N + R Q++ +L ++L+FA W+P + +LMI F S + + +T+ V L+ L + +P +Y ++N FK+ LIK L
Sbjct: 79 IFLLGILGNSAVLYLTMKHRQLQTVQNIFILNLCASNVLMCLTSLPITFITNVYKQWFFSSPVCKLIPLVQGASIFVSTFSLSAIALDRYNLVVRPHKQKLSSRSAMMIALLIWVISVVVCMPYGWYMDVEKLNGLCG---------EYCSEHWPLAEVRKGYTFLVLITQFLFPFATMAFCYYNIFSRLRQRVETKLKKLSERSQLLENSTTCGTTNHIVSINAEAVQNGLENKQRLAVLAQQRRTTTILSCMVLLFAFTWLPHNVVTLMIEYDGFFFHSDETSATSTDHTYIVSMTAHLISMLTNVTNPFLYAWLNPMFKEMLIKTL 398
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Fly
Match: RYa-R (gene:FBgn0004842 transcript:FBtr0345268) HSP 1 Score: 108.227 bits (269), Expect = 1.081e-25 Identity = 80/320 (25.00%), Postives = 141/320 (44.06%), Query Frame = 1
Query: 103 IFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCR-SIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKSYQNKRVNSGFPGLLIL-VWIISTVATIPVLYFRKTNRLGEYLNISL-PLETSIAY--CFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATEVLPYTLL----LGYLHSSMSPIVYWFINSSFKQSLIKLLGQ 1035
IF+ ++IGNG V + RMR N + +L++ D+ ++ C+P+ I YW G +C Y+Q V + S+ +L +SI R + R+ + +I VW I+ +P+ + L+I + P T C E W ++ Y +S+ A+ F++PL V+I TY+ I +V E N R R K+ MV M++ +++VF CW+PF + L++ D + + LPY L H +PI+Y ++N+ F+ ++L+ +
Sbjct: 115 IFIFALIGNGTVCYIVYSTPRMRTVTNYFIASLAIGDILMSFFCVPSSFISLFILNYWPFGLALCHFVNYSQAVSVLVSAYTLVAISIDRYIAIMWPLKPRITKRYATFIIAGVWFIALATALPI-------PIVSGLDIPMSPWHTKCEKYICREMWPSRTQEYYYTLSLFALQFVVPLGVLIFTYARITIRVWA-----------------------KRPPGEAETNRDQRMARSKRKMVKMMLTVVIVFTCCWLPFNILQLLLN----DEEFAHWDPLPYVWFAFHWLAMSHCCYNPIIYCYMNARFRSGFVQLMHR 400
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Fly
Match: RYa-R (gene:FBgn0004842 transcript:FBtr0085100) HSP 1 Score: 107.071 bits (266), Expect = 2.269e-25 Identity = 80/320 (25.00%), Postives = 141/320 (44.06%), Query Frame = 1
Query: 103 IFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCR-SIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKSYQNKRVNSGFPGLLIL-VWIISTVATIPVLYFRKTNRLGEYLNISL-PLETSIAY--CFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATEVLPYTLL----LGYLHSSMSPIVYWFINSSFKQSLIKLLGQ 1035
IF+ ++IGNG V + RMR N + +L++ D+ ++ C+P+ I YW G +C Y+Q V + S+ +L +SI R + R+ + +I VW I+ +P+ + L+I + P T C E W ++ Y +S+ A+ F++PL V+I TY+ I +V E N R R K+ MV M++ +++VF CW+PF + L++ D + + LPY L H +PI+Y ++N+ F+ ++L+ +
Sbjct: 115 IFIFALIGNGTVCYIVYSTPRMRTVTNYFIASLAIGDILMSFFCVPSSFISLFILNYWPFGLALCHFVNYSQAVSVLVSAYTLVAISIDRYIAIMWPLKPRITKRYATFIIAGVWFIALATALPI-------PIVSGLDIPMSPWHTKCEKYICREMWPSRTQEYYYTLSLFALQFVVPLGVLIFTYARITIRVWA-----------------------KRPPGEAETNRDQRMARSKRKMVKMMLTVVIVFTCCWLPFNILQLLL----NDEEFAHWDPLPYVWFAFHWLAMSHCCYNPIIYCYMNARFRSGFVQLMHR 400
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Fly
Match: RYa-R (gene:FBgn0004842 transcript:FBtr0345267) HSP 1 Score: 106.686 bits (265), Expect = 5.219e-25 Identity = 80/320 (25.00%), Postives = 141/320 (44.06%), Query Frame = 1
Query: 103 IFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCR-SIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKSYQNKRVNSGFPGLLIL-VWIISTVATIPVLYFRKTNRLGEYLNISL-PLETSIAY--CFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATEVLPYTLL----LGYLHSSMSPIVYWFINSSFKQSLIKLLGQ 1035
IF+ ++IGNG V + RMR N + +L++ D+ ++ C+P+ I YW G +C Y+Q V + S+ +L +SI R + R+ + +I VW I+ +P+ + L+I + P T C E W ++ Y +S+ A+ F++PL V+I TY+ I +V E N R R K+ MV M++ +++VF CW+PF + L++ D + + LPY L H +PI+Y ++N+ F+ ++L+ +
Sbjct: 115 IFIFALIGNGTVCYIVYSTPRMRTVTNYFIASLAIGDILMSFFCVPSSFISLFILNYWPFGLALCHFVNYSQAVSVLVSAYTLVAISIDRYIAIMWPLKPRITKRYATFIIAGVWFIALATALPI-------PIVSGLDIPMSPWHTKCEKYICREMWPSRTQEYYYTLSLFALQFVVPLGVLIFTYARITIRVWA-----------------------KRPPGEAETNRDQRMARSKRKMVKMMLTVVIVFTCCWLPFNILQLLL----NDEEFAHWDPLPYVWFAFHWLAMSHCCYNPIIYCYMNARFRSGFVQLMHR 400
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Fly
Match: ETHR (gene:FBgn0038874 transcript:FBtr0084053) HSP 1 Score: 98.5969 bits (244), Expect = 2.379e-22 Identity = 90/332 (27.11%), Postives = 158/332 (47.59%), Query Frame = 1
Query: 73 RLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCR--SIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISR-LLVTKSYQNKRVNSGFPGLLI--LVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSRE---YRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATEVLPYTLL-----LGYLHSSMSPIVYWFINSSFKQSLIKLL 1029
R + C +I LL V+GN +V I+ +K K MR S NI L NLS+ADL V LVC P L + W+LG MCK + + + +S L++ +S R + + + V + +LI L W I+ + T P++ T + Y + T C D F + Y++ + + F LP ++++ Y+ I +K+ R + G + S+ T N+H + + +K ++ ML+ ++ F +C +PF+ +L ++ S ++ Y LL + YL+S+M+PI+Y ++S F+ +LL
Sbjct: 10 RTTAMFFCIVIMLLGVVGNVMVPIVIVKTKDMRNSTNIFLTNLSIADLLVLLVCTPTVLVEVNTRPETWVLGHEMCKAVPFVELTVAHASVLTILAISFERYYAICEPLKAGYVCTKGRAILICVLAWGIAALFTSPIIAI-STYSVEPYGD-----GTDAPVCTTAADGF-WSIFYFVGCITVFFFLPFGILVLLYAAIAYKLLRPNNAFHRPTSPQPQQPSGGATSGSSQVPSTKGNSHQQSNGMRKHRKQVIFMLVAVVSSFFVCLLPFRAFTLWVILASAEDVEGLGIAGYYNLLYFSRFMLYLNSAMNPILYNLMSSKFRSGFWRLL 334
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Fly
Match: SIFaR (gene:FBgn0038880 transcript:FBtr0084098) HSP 1 Score: 93.5893 bits (231), Expect = 1.666e-20 Identity = 97/359 (27.02%), Postives = 171/359 (47.63%), Query Frame = 1
Query: 7 FNNSAV---VLDIIYSQYFQPLTRWRL----VYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVT----KSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAY--CFEKWDKFEYQFIYY-ISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATEVL----PYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLL 1029
+N+SA+ LD F PL R L VY ++FL+ +IGN V+ + L+ RMR N ++NL++AD+ V + C+PA L +I+ W+LG +MCK Y QGV + +S SL +S+ R + K +R ++I +W+I+ V TIP L F E + +L S C E W +Y+ ++ L ++LP+ ++ + Y I KVS R+ SK+ ++ ++ + K ++ ML+ ++++F + W+P + I F S D + + E+L P LG +S ++PI+Y +N +++ ++
Sbjct: 179 YNDSALRWEQLDGSVDFGFDPLYRHSLAMSMVYCVAYIVVFLVGLIGNSFVIAVVLRAPRMRTVTNYFIVNLAIADILVIVFCLPATLIGNIFVPWMLGWLMCKFVPYIQGVSVAASVYSLIAVSLDRFIAIWWPLKQMTKRRARI----MIIGIWVIALVTTIPWLLFFDLVPAEEVFSDALVSAYSQPQFLCQEVWPPGTDGNLYFLLANLVACYLLPMSLITLCYVLIWIKVS--------------------TRSIPGESKDAQMDRMQQKSKVK--VIKMLVAVVILFVLSWLPLYVIFARIKFGS-DISQEEFEILKKVMPVAQWLGSSNSCINPILY-SVNKKYRRGFAAII 509
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Zebrafish
Match: npy2rl (neuropeptide Y receptor Y2, like [Source:ZFIN;Acc:ZDB-GENE-111115-4]) HSP 1 Score: 112.464 bits (280), Expect = 1.542e-27 Identity = 89/326 (27.30%), Postives = 161/326 (49.39%), Query Frame = 1
Query: 73 RLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKSYQNKRVNSGFPGLLI-LVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKW-DKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATEVLPYTLLLGYLH------SSMSPIVYWFINSSFKQSLIKL 1026
++V I S I LL V+GN LV+ + KFK +R N + NL++ADL V +C+P L ++ W G+V+C YAQG+ + S+++L +++ R + + R++ L+I + W++S V P+ FR EY + L + SI C EKW D +Y IS L + ++LPL II++++I ++ LRNH + + + R +R++K ML+ +++VFA+ W+PF L I D + ++ + LL H + +P++Y ++N +++ + + +
Sbjct: 44 QVVLILAYSTIILLGVVGNSLVIYVVYKFKTLRTVTNFFIANLAVADLLVNTLCLPFTLAYTLLREWKFGQVLCFTLPYAQGLAVHVSTITLNVIALDRHRCIVYHLDTRMSKDTCFLVIAITWVVSAVLASPLAIFR------EYGIVDLSPDNSIEVCGEKWPDSSTDSTLYSISTLLLQYVLPL--AIISFAYI--------------------RIWSKLRNHV-----SPVGRNDRHHRRRK-TTKMLVTMVVVFAVSWLPFHAFQLAI-----DIDHSVLDMKDFRLLYTVFHIVAMCSTFANPLLYGWMNRNYRSAFVAV 330
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Zebrafish
Match: si:dkey-1h24.2 (si:dkey-1h24.2 [Source:ZFIN;Acc:ZDB-GENE-060503-482]) HSP 1 Score: 111.309 bits (277), Expect = 7.031e-27 Identity = 106/379 (27.97%), Postives = 184/379 (48.55%), Query Frame = 1
Query: 46 QYFQPLTRWRL--VYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL------LVTKSYQNKRVNSGFPGLLILVWIISTVATIP------VLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKV-------LGYLRNHSN-----LSKE----------TSLNAHSREYRQ---------KKFMVGMLIILILVFAICWMP-FQLNSLMILFMSFD--NAYKATEVLPYTL--LLGYLHSSMSPIVYWFINSSFKQSLIKLLG 1032
Q F+P + V I L SL+FLLSV GN L++++ + KRMR N LL+L+++DL + + CIP L S+ +I G MCK Y G+ ++ S+ SL ++I R L ++++Q + S ++ W++S +P ++ F KTN + ++ C +W + + +Y+ +L+I F +P VMII Y I ++ R I+ L +D + V L + S+ +SK+ TS + R KK ++ MLII++ +F +CWMP + N+ + +FD +A +A P + LL Y + ++PI+Y F+N+ F+++L+ G
Sbjct: 21 QKFKPTRPKEMDSVRIILYSLVFLLSVFGNLLIIVVLIVNKRMRTVTNSFLLSLAVSDLMMAIFCIPFNLIPSLLEDFIFGAAMCKTVAYFMGISVSISTFSLVAIAIERYSAICNPLKSRAWQTR---SHAYKVIAATWVLSVAIMVPYPVFSILVPFNKTNNITAHM------------CRLEWPISQVEQTWYVLLLSILFFIPGVVMIIAYGLISRELYRGIQFEL-EQKKDTSGVKNGLNGGLSSCHDESDGCYIQVSKKPPAMELSTLTTSGTVKTDRPRSNTSEAKLIAKKRVIRMLIIIVAMFFVCWMPLYTANT----WKAFDQISAQRALSGAPISFIQLLSYTSACVNPIIYCFMNTRFRKALLATFG 379
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Zebrafish
Match: hcrtr2 (hypocretin (orexin) receptor 2 [Source:ZFIN;Acc:ZDB-GENE-070228-3]) HSP 1 Score: 111.309 bits (277), Expect = 7.571e-27 Identity = 90/347 (25.94%), Postives = 165/347 (47.55%), Query Frame = 1
Query: 10 NNSAVVLDIIYSQYFQPLTRWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL-LVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKV-------SRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQ-LNSLMILFMSFDNAYKATEV---LPYTLLLGYLHSSMSPIVYWFINSSFKQS 1014
N +L I+ +Y P ++ V I L+FL+S++GN LV K MR N ++NLS AD+ VT+ C+PA L I W G+ +CKI Y Q + ++ S L+L ++ R + + K ++L+W++S + IP +++ L L L T C E+W Y +Y+ + + PL +M++ Y I K+ S + R Q +G S + ++++A +++ + ++ ML++++ VFA+C++P LN + +F +F N V ++ L Y +S+ +PI+Y F++ F++
Sbjct: 34 NEDDELLKYIWREYLHP-KQYEWVLIAGYILVFLVSLVGNTLVCFAVWKNHHMRTVTNYFIVNLSFADILVTITCLPASLVVDITETWFFGQTLCKILPYLQTISVSVSVLTLSCIAQDRWYAICHPLKFKSTAKRARKSIVLIWLVSCIMMIPQAVVMESSSLMPELTNKTSLFT---VCDEQWPDEIYPKVYHTCFFIVTYFAPLCLMVLAYIQICHKLWCQQIPGSSSVLQRQWKSLQCSAHAVG--SGESVKVRTSTVSAEAKQVKARRKTARMLMVVLFVFALCYLPISILNIMKRVFGAFKNTGNRETVYAWFTFSHWLIYANSAANPIIYNFLSGKFREE 374
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Zebrafish
Match: si:dkey-1h24.2 (si:dkey-1h24.2 [Source:ZFIN;Acc:ZDB-GENE-060503-482]) HSP 1 Score: 110.538 bits (275), Expect = 1.477e-26 Identity = 104/379 (27.44%), Postives = 182/379 (48.02%), Query Frame = 1
Query: 46 QYFQPLTRWRL--VYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISR------LLVTKSYQNKRVNSGFPGLLILVWIISTVATIP------VLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKV-------LGYLRNHSN-----LSKETSL-------------------NAHSREYRQKKFMVGMLIILILVFAICWMP-FQLNSLMILFMSFD--NAYKATEVLPYTL--LLGYLHSSMSPIVYWFINSSFKQSLIKLLG 1032
Q F+P + V I L SL+FLLSV GN L++++ + KRMR N LL+L+++DL + + CIP L S+ +I G MCK Y G+ ++ S+ SL ++I R L ++++Q + S ++ W++S +P ++ F KTN + ++ C +W + + +Y+ +L+I F +P VMII Y I ++ R I+ L +D + V L + S+ +SK+ N + KK ++ MLII++ +F +CWMP + N+ + +FD +A +A P + LL Y + ++PI+Y F+N+ F+++L+ G
Sbjct: 45 QKFKPTRPKEMDSVRIILYSLVFLLSVFGNLLIIVVLIVNKRMRTVTNSFLLSLAVSDLMMAIFCIPFNLIPSLLEDFIFGAAMCKTVAYFMGISVSISTFSLVAIAIERYSAICNPLKSRAWQTR---SHAYKVIAATWVLSVAIMVPYPVFSILVPFNKTNNITAHM------------CRLEWPISQVEQTWYVLLLSILFFIPGVVMIIAYGLISRELYRGIQFEL-EQKKDTSGVKNGLNGGLSSCHDESDGCYIQVSKKPPAMELSTLTTSGPVKTDRPRSNTSEAKLIAKKRVIRMLIIIVAMFFVCWMPLYSANT----WKAFDQISAQRALSGAPISFIQLLSYTSACVNPIIYCFMNTRFRKALLATFG 403
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Zebrafish
Match: qrfpr4 (pyroglutamylated RFamide peptide receptor 4 [Source:ZFIN;Acc:ZDB-GENE-091204-354]) HSP 1 Score: 107.457 bits (267), Expect = 2.377e-25 Identity = 80/317 (25.24%), Postives = 160/317 (50.47%), Query Frame = 1
Query: 100 LIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISR---LLVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMI----LFMSFDNAYKATEVLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLL 1029
LIF L+V+GNG V+++ + K + + +L+L+DL +++VC+PA L + +T W+ G+ +CK+ + Q I++S L++ +++ R +L +N +L+ VWI++ P+ + +K ++L + C E W + + +Y + + F+ PL M I Y +K+ R ++ G + H + + + ++ R+KK + M+ ++L+FA CW PF L SL++ L + D+ + V+ +LG+ +S +P++Y +N++FK+ L+ LL
Sbjct: 51 LIFGLAVVGNGAVLVILCRRKALESPSTFFICSLALSDLLISIVCVPATLLQHFFTNWLAGDFLCKLIPFLQVTAISTSMLTMTCIAVERFQGILYPLHVRNSYFLCHAFKMLVTVWIVALAIAAPMWFVQKVEVKYDFL-----FDVHHTCCLEVWPNQQQRRVYTTCLCVLVFLAPLATMAILY----WKIMR--------------ELWGNHKVHDIMFQTLPGSEINKITRRKKRAIRMMATVVLLFAACWAPFHLVSLLLDYGQLNLDLDDEFLLVSVV---QILGFSNSVCNPLIYAALNTTFKRDLLALL 341
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Xenopus
Match: slc11a2 (solute carrier family 11 member 2 [Source:Xenbase;Acc:XB-GENE-487563]) HSP 1 Score: 124.02 bits (310), Expect = 4.465e-31 Identity = 95/331 (28.70%), Postives = 174/331 (52.57%), Query Frame = 1
Query: 67 RWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISR-------LLVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATE---VLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLL 1029
R ++ ++T+C LIF+L++ GN LV+ + + K MR NI + +L+L+DL + CIP + ++I + W+ G CK+ + Q I + L++ +++ R L + Y N+R + +L +VW+I+ V IP+ + ++ ++L E C E W+ +Q IY +L I F+LPL VM++ YS K+ + I+ V D + + + S +SK R+KK + M+I ++++FA+CW PF + +MI + +F+N Y + ++G+ +S +PIVY F+N +FK++ + L
Sbjct: 77 RTKIAFVTICVLIFVLALFGNSLVLYVVTRSKAMRTVTNIFICSLALSDLLIAFFCIPFTMLQNISSNWLGGAFACKMVPFVQSTAIVTEILTMTCIAVERHQGIVHPLKMKWQYTNRRAFT----MLGIVWLIAAVVGIPMWHAQRLEVKYDFL-----YEKQYVCCLEAWNSQVHQKIYTTFILVILFLLPLTVMLLLYS----KIGYELWIKKRVGDASVLQTI----HGSEMSKIA---------RKKKRAIIMMITVVVLFAVCWAPFHVVHMMIEYSNFENEYDDVTIKIIFAIVQIIGFFNSICNPIVYAFMNENFKKNFLSAL 381
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Xenopus
Match: taf1b (TATA-box binding protein associated factor, RNA polymerase I, B [Source:Xenbase;Acc:XB-GENE-992275]) HSP 1 Score: 117.472 bits (293), Expect = 2.871e-29 Identity = 91/331 (27.49%), Postives = 163/331 (49.24%), Query Frame = 1
Query: 73 RLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKSYQNKRVNSGFPGLLI-LVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATEVLPYTL--LLGYLHSSMSPIVYWFINSSFKQSLIKLLGQQ*EIHNA 1056
+++ I SLI LL ++GN LV+ + +K+K MR N + NL++ADL V C+P L ++ W G V+C + YAQ + + S+L+L +++ R + N R++ L+I + WI + + IP+ FR+ Y ++ P IA C E W + IY +SML + + LPL V+ Y I FK L+NH S S +++K ML+++++VFA+CW+PF + L I + + A ++L YT+ ++ + ++P +Y ++N +++ + G + + N+
Sbjct: 39 QVILIAAYSLIILLGLVGNSLVIYMIVKYKNMRTVTNFFIANLAIADLMVDSFCLPFTLVYTLMDEWKFGSVLCHLFPYAQAMSVNVSTLTLIVIALDRYWCIVFHLNSRISKNLSFLIISITWITAAILAIPLAVFREF----RYEDLP-PFNLKIAVCAENWTNRD-STIYSLSMLILQYALPLAVICYAYLRIWFK----------------------LKNH------ISPTTRSESQQRRKNTTKMLVMMVVVFAVCWLPFHIFQLAI-DLEWTVAIHENKLL-YTIFHVVAMCSTFVNPFLYGWMNKNYRHGFLTFFGCKDTLQNS 333
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Xenopus
Match: HCRTR2 (hypocretin (orexin) receptor 2 [Source:NCBI gene;Acc:100495420]) HSP 1 Score: 108.612 bits (270), Expect = 1.248e-25 Identity = 94/340 (27.65%), Postives = 170/340 (50.00%), Query Frame = 1
Query: 37 IYSQYFQPLTRWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL------LVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKV-SRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQ---KKFMVGMLIILILVFAICWMPFQ-LNSLMILFMSFDNAYKATEV---LPYTLLLGYLHSSMSPIVYWFINSSFKQS 1014
++ +Y P ++ V I ++F++++IGN LV + K MR N ++NLSLAD+ VT++C+PA L I W G+ +CK+ Y Q V ++ S L+L +++ R L+ KS + S ++++WI+S IP + + L L T C E+W+ Y +Y+I I +++PL +MI+ Y I K+ R I V + + +++ S ++ NA + E +Q ++ ML++++LVFA+C++P LN L +F F + V ++ L Y +S+ +PI+Y F++ F++
Sbjct: 41 LWREYLHP-KQYEWVLIVGYIIVFIIALIGNILVCVAVWKNHHMRTVTNYFIVNLSLADVLVTIICLPATLLVDITETWFFGKTLCKVIPYLQTVSVSVSVLTLSCIALDRWYAICHPLMFKSTAKRAQQS-----IVIIWIVSCAIMIPQAIVMECRSVFPELANKTILFT---VCDERWEGQIYSKVYHICFFCITYMVPLCLMILAYLQIFRKLWCRQIPGTSSVVQKKWKPLQCSIQSKGQQSTKSRNNAVAAEIKQIHARRKTARMLMVVLLVFALCYLPISILNILKRVFGMFTHTNDRETVYAWFTFSHWLVYANSAANPIIYNFLSGKFREE 371
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Xenopus
Match: clstn2 (calsyntenin 2 [Source:Xenbase;Acc:XB-GENE-958942]) HSP 1 Score: 102.834 bits (255), Expect = 8.979e-24 Identity = 93/351 (26.50%), Postives = 174/351 (49.57%), Query Frame = 1
Query: 31 DIIYSQYFQPLTRWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL-LVTKSYQNKRVNSGFPGLLILVWIISTVATIP---VLYFRKTN---RLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITY---SFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMIL-----FMSFDNAYKATEVLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLLGQQ 1038
+ +S Y+Q T ++I + IFL+ +IGN LV + LK ++MR N+ +LNL+++DL V + C+P L ++ T W +MCK+S QG+ +++S +L +++ R + ++ K +I++W+++ + P L K + + +Y N PL + C+E+W E + IY + + ++ PL +++I Y +F +FK S IR ++S+E N R ++K ++ MLII+ L F++ W+P L +LM+L + A V P+ L + +SS++PI+Y + N +F++ + Q
Sbjct: 23 NFTFSLYYQHSTSIAAMFILAYTFIFLMCMIGNMLVCFIVLKNRQMRTVTNMFILNLAISDLLVGIFCMPTTLVDNLITGWPFDNIMCKMSGLVQGMSVSASVFTLVAIAVERFRCIVYPFRQKLTLRKAIITIIIIWVLALIIMCPSAVTLTVTKDDFHFMVDDYNNY-YPLYS----CWEEWPDTEMRKIYTTVLFSHIYLAPLTLIVIMYARIAFKLFKSSAPIR--------------------GSVSEE---NEGRRVSKRKVRVIYMLIIVALFFSLSWLP--LWTLMLLTDYGHLSDYQLNLIAVYVFPFAHWLAFFNSSVNPIIYGYFNENFRRGFQEAFKVQ 343
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Xenopus
Match: clstn2 (calsyntenin 2 [Source:Xenbase;Acc:XB-GENE-958942]) HSP 1 Score: 102.834 bits (255), Expect = 1.121e-23 Identity = 93/351 (26.50%), Postives = 174/351 (49.57%), Query Frame = 1
Query: 31 DIIYSQYFQPLTRWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL-LVTKSYQNKRVNSGFPGLLILVWIISTVATIP---VLYFRKTN---RLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITY---SFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMIL-----FMSFDNAYKATEVLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLLGQQ 1038
+ +S Y+Q T ++I + IFL+ +IGN LV + LK ++MR N+ +LNL+++DL V + C+P L ++ T W +MCK+S QG+ +++S +L +++ R + ++ K +I++W+++ + P L K + + +Y N PL + C+E+W E + IY + + ++ PL +++I Y +F +FK S IR ++S+E N R ++K ++ MLII+ L F++ W+P L +LM+L + A V P+ L + +SS++PI+Y + N +F++ + Q
Sbjct: 24 NFTFSLYYQHSTSIAAMFILAYTFIFLMCMIGNMLVCFIVLKNRQMRTVTNMFILNLAISDLLVGIFCMPTTLVDNLITGWPFDNIMCKMSGLVQGMSVSASVFTLVAIAVERFRCIVYPFRQKLTLRKAIITIIIIWVLALIIMCPSAVTLTVTKDDFHFMVDDYNNY-YPLYS----CWEEWPDTEMRKIYTTVLFSHIYLAPLTLIVIMYARIAFKLFKSSAPIR--------------------GSVSEE---NEGRRVSKRKVRVIYMLIIVALFFSLSWLP--LWTLMLLTDYGHLSDYQLNLIAVYVFPFAHWLAFFNSSVNPIIYGYFNENFRRGFQEAFKVQ 344
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Mouse
Match: Hcrtr2 (hypocretin (orexin) receptor 2 [Source:MGI Symbol;Acc:MGI:2680765]) HSP 1 Score: 108.227 bits (269), Expect = 1.302e-25 Identity = 94/344 (27.33%), Postives = 169/344 (49.13%), Query Frame = 1
Query: 28 LDIIYSQYFQPLTRWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL------LVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKV--SRLIRIRLIVDDQ-DHTKVLGYLRNHSNLSKE--TSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQ-LNSLMILFMSFDNAYKATEV---LPYTLLLGYLHSSMSPIVYWFINSSFKQS 1014
L ++ +Y P + V I ++F++++IGN LV + K MR N ++NLSLAD+ VT+ C+PA L I W G+ +CK+ Y Q V ++ S L+L +++ R L+ KS + NS ++++WI+S + IP + + + L L T C E W Y +Y+I + ++ PL +MI+ Y I K+ ++ +V + + + R SK +++ A ++ R ++ ML++++LVFAIC++P LN L +F F + V ++ L Y +S+ +PI+Y F++ F++
Sbjct: 40 LRYLWREYLHP-KEYEWVLIAGYIIVFVVALIGNVLVCVAVWKNHHMRTVTNYFIVNLSLADVLVTITCLPATLVVDITETWFFGQSLCKVIPYLQTVSVSVSVLTLSCIALDRWYAICHPLMFKSTAKRARNS-----IVVIWIVSCIIMIPQAIVMECSSMLPGLANKTTLFT---VCDEHWGGEVYPKMYHICFFLVTYMAPLCLMILAYLQIFRKLWCRQIPGTSSVVQRKWKQQQPVSQPRGSGQQSKARISAVAAEIKQIRARRKTARMLMVVLLVFAICYLPISILNVLKRVFGMFTHTEDRETVYAWFTFSHWLVYANSAANPIIYNFLSGKFREE 374
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Mouse
Match: Hcrtr2 (hypocretin (orexin) receptor 2 [Source:MGI Symbol;Acc:MGI:2680765]) HSP 1 Score: 108.227 bits (269), Expect = 1.538e-25 Identity = 94/344 (27.33%), Postives = 169/344 (49.13%), Query Frame = 1
Query: 28 LDIIYSQYFQPLTRWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL------LVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKV--SRLIRIRLIVDDQ-DHTKVLGYLRNHSNLSKE--TSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQ-LNSLMILFMSFDNAYKATEV---LPYTLLLGYLHSSMSPIVYWFINSSFKQS 1014
L ++ +Y P + V I ++F++++IGN LV + K MR N ++NLSLAD+ VT+ C+PA L I W G+ +CK+ Y Q V ++ S L+L +++ R L+ KS + NS ++++WI+S + IP + + + L L T C E W Y +Y+I + ++ PL +MI+ Y I K+ ++ +V + + + R SK +++ A ++ R ++ ML++++LVFAIC++P LN L +F F + V ++ L Y +S+ +PI+Y F++ F++
Sbjct: 40 LRYLWREYLHP-KEYEWVLIAGYIIVFVVALIGNVLVCVAVWKNHHMRTVTNYFIVNLSLADVLVTITCLPATLVVDITETWFFGQSLCKVIPYLQTVSVSVSVLTLSCIALDRWYAICHPLMFKSTAKRARNS-----IVVIWIVSCIIMIPQAIVMECSSMLPGLANKTTLFT---VCDEHWGGEVYPKMYHICFFLVTYMAPLCLMILAYLQIFRKLWCRQIPGTSSVVQRKWKQQQPVSQPRGSGQQSKARISAVAAEIKQIRARRKTARMLMVVLLVFAICYLPISILNVLKRVFGMFTHTEDRETVYAWFTFSHWLVYANSAANPIIYNFLSGKFREE 374
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Mouse
Match: Hcrtr1 (hypocretin (orexin) receptor 1 [Source:MGI Symbol;Acc:MGI:2385650]) HSP 1 Score: 101.293 bits (251), Expect = 2.639e-23 Identity = 86/330 (26.06%), Postives = 154/330 (46.67%), Query Frame = 1
Query: 103 IFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISR-------LLVTKSYQNKRVNSGFPGLLILVWIISTVATIP-VLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFK----------VSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHS-----REYRQKKFMVGMLIILILVFAICWMPFQ-LNSLMILFMSFDNAYKATEV---LPYTLLLGYLHSSMSPIVYWFINSSFKQ 1011
+FL++++GN LV + + MR N ++NLSLAD+ VT +C+PA L I W+ G+ +CK+ Y Q V ++ + L+L +++ R LL + + R G ++ +W +S +P ++ L E N + + C E W Y IY+ + ++ PL +M + Y F +F+ S L+R +Q + H L E A + ++ R ++ ML++++LVFA+C++P LN L +F F A V ++ L Y +S+ +PI+Y F++ F++
Sbjct: 56 VFLIALVGNTLVCLAVWRNHHMRTVTNYFIVNLSLADVLVTAICLPASLLVDITESWLFGQALCKVIPYLQAVSVSVAVLTLSFIALDRWYAICHPLLFKSTARRAR------GSILGIWAVSLAVMVPQAAVMECSSVLPELANRT----RLFSVCDEHWADELYPKIYHSCFFIVTYLAPLGLMAMAY-FQIFRKLWGRQIPGTTSALVRNWKRPSEQLEAQ-------HQGLCTEPQPRARAFLAEVKQMRARRKTAKMLMVVLLVFALCYLPISVLNVLKRVFGMFRQASDREAVYACFTFSHWLVYANSAANPIIYNFLSGKFRE 367
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Mouse
Match: Hcrtr1 (hypocretin (orexin) receptor 1 [Source:MGI Symbol;Acc:MGI:2385650]) HSP 1 Score: 101.293 bits (251), Expect = 2.639e-23 Identity = 86/330 (26.06%), Postives = 154/330 (46.67%), Query Frame = 1
Query: 103 IFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISR-------LLVTKSYQNKRVNSGFPGLLILVWIISTVATIP-VLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFK----------VSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHS-----REYRQKKFMVGMLIILILVFAICWMPFQ-LNSLMILFMSFDNAYKATEV---LPYTLLLGYLHSSMSPIVYWFINSSFKQ 1011
+FL++++GN LV + + MR N ++NLSLAD+ VT +C+PA L I W+ G+ +CK+ Y Q V ++ + L+L +++ R LL + + R G ++ +W +S +P ++ L E N + + C E W Y IY+ + ++ PL +M + Y F +F+ S L+R +Q + H L E A + ++ R ++ ML++++LVFA+C++P LN L +F F A V ++ L Y +S+ +PI+Y F++ F++
Sbjct: 56 VFLIALVGNTLVCLAVWRNHHMRTVTNYFIVNLSLADVLVTAICLPASLLVDITESWLFGQALCKVIPYLQAVSVSVAVLTLSFIALDRWYAICHPLLFKSTARRAR------GSILGIWAVSLAVMVPQAAVMECSSVLPELANRT----RLFSVCDEHWADELYPKIYHSCFFIVTYLAPLGLMAMAY-FQIFRKLWGRQIPGTTSALVRNWKRPSEQLEAQ-------HQGLCTEPQPRARAFLAEVKQMRARRKTAKMLMVVLLVFALCYLPISVLNVLKRVFGMFRQASDREAVYACFTFSHWLVYANSAANPIIYNFLSGKFRE 367
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Mouse
Match: Hcrtr1 (hypocretin (orexin) receptor 1 [Source:MGI Symbol;Acc:MGI:2385650]) HSP 1 Score: 101.293 bits (251), Expect = 2.639e-23 Identity = 86/330 (26.06%), Postives = 154/330 (46.67%), Query Frame = 1
Query: 103 IFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISR-------LLVTKSYQNKRVNSGFPGLLILVWIISTVATIP-VLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFK----------VSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHS-----REYRQKKFMVGMLIILILVFAICWMPFQ-LNSLMILFMSFDNAYKATEV---LPYTLLLGYLHSSMSPIVYWFINSSFKQ 1011
+FL++++GN LV + + MR N ++NLSLAD+ VT +C+PA L I W+ G+ +CK+ Y Q V ++ + L+L +++ R LL + + R G ++ +W +S +P ++ L E N + + C E W Y IY+ + ++ PL +M + Y F +F+ S L+R +Q + H L E A + ++ R ++ ML++++LVFA+C++P LN L +F F A V ++ L Y +S+ +PI+Y F++ F++
Sbjct: 56 VFLIALVGNTLVCLAVWRNHHMRTVTNYFIVNLSLADVLVTAICLPASLLVDITESWLFGQALCKVIPYLQAVSVSVAVLTLSFIALDRWYAICHPLLFKSTARRAR------GSILGIWAVSLAVMVPQAAVMECSSVLPELANRT----RLFSVCDEHWADELYPKIYHSCFFIVTYLAPLGLMAMAY-FQIFRKLWGRQIPGTTSALVRNWKRPSEQLEAQ-------HQGLCTEPQPRARAFLAEVKQMRARRKTAKMLMVVLLVFALCYLPISVLNVLKRVFGMFRQASDREAVYACFTFSHWLVYANSAANPIIYNFLSGKFRE 367
BLAST of Orphan G-protein coupled receptor 22 vs. UniProt/SwissProt
Match: sp|Q96P65|QRFPR_HUMAN (Pyroglutamylated RF-amide peptide receptor OS=Homo sapiens OX=9606 GN=QRFPR PE=1 SV=2) HSP 1 Score: 119.398 bits (298), Expect = 8.359e-29 Identity = 90/328 (27.44%), Postives = 164/328 (50.00%), Query Frame = 1
Query: 67 RWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISR-------LLVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATE---VLPYTLLLGYLHSSMSPIVYWFINSSFKQSLI 1020
R +L + LIF L++ GN LV + + K MR NI + +L+L+DL +T CIP + ++I W+ G +CK+ + Q + + L++ +++ R + Y N+R + +L +VW+++ + P+ + ++ ++L E C E+W +Q IY +L I F+LPL VM+I YS K+ + I+ V D + + KE S A R+KK V M++ ++ +FA+CW PF + +MI + +F+ Y + ++G+ +S +PIVY F+N +FK++++
Sbjct: 43 RAKLALVLTGVLIFALALFGNALVFYVVTRSKAMRTVTNIFICSLALSDLLITFFCIPVTMLQNISDNWLGGAFICKMVPFVQSTAVVTEILTMTCIAVERHQGLVHPFKMKWQYTNRRAFT----MLGVVWLVAVIVGSPMWHVQQLEIKYDFL-----YEKEHICCLEEWTSPVHQKIYTTFILVILFLLPLMVMLILYS----KIGYELWIKKRVGDGSVLRTIH--------GKEMSKIA-----RKKKRAVIMMVTVVALFAVCWAPFHVVHMMIEYSNFEKEYDDVTIKMIFAIVQIIGFSNSICNPIVYAFMNENFKKNVL 344
BLAST of Orphan G-protein coupled receptor 22 vs. UniProt/SwissProt
Match: sp|C3ZQF9|QRFPR_BRAFL (QRFP-like peptide receptor OS=Branchiostoma floridae OX=7739 GN=QRFPR PE=3 SV=1) HSP 1 Score: 115.161 bits (287), Expect = 1.129e-27 Identity = 94/316 (29.75%), Postives = 158/316 (50.00%), Query Frame = 1
Query: 100 LIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLV-----TKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATEVL-PYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLL 1029
+IFL +++GN LVV++ K K MR + NI + +L+ +DL +T+VCIP L +++ WI+G+ MCK+ + Q + + SS L+L G++I R Y + +G +L LVW++S P+L+ K + ++L E C EKW Q Y I L + FI+PL M Y IRI + Q V G AH R+K+ V ML++++L+FA+CW+P+ ++M K+ ++L L+ + +S +P+VY +N +FK++ + +L
Sbjct: 56 IIFLAALLGNTLVVVVVWKNKVMRTTMNIFICSLAASDLLITIVCIPVTLMQNMLQNWIMGDFMCKLVPFIQTIAVASSILTLTGIAIERYYAIIHPLKVKYLLSKTRAGI--ILALVWVVSVGVATPMLFVHKAEEIHDFL-----YEQRFVTCQEKWWGQTQQTSYTIFNLVVLFIIPLLTMTSLY----------IRIAHRLWVQQPVGVTGNF-------------AHGNSVRRKRQAVKMLVVVVLLFAVCWLPYHTVTVMNELTGLRLEEKSAKLLIAIVQLIAFSNSFNNPVVYAILNENFKKNFMTML 341
BLAST of Orphan G-protein coupled receptor 22 vs. UniProt/SwissProt
Match: sp|P56719|OX2R_RAT (Orexin receptor type 2 OS=Rattus norvegicus OX=10116 GN=Hcrtr2 PE=2 SV=1) HSP 1 Score: 110.923 bits (276), Expect = 1.172e-25 Identity = 93/345 (26.96%), Postives = 172/345 (49.86%), Query Frame = 1
Query: 28 LDIIYSQYFQPLTRWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL------LVTKSYQNKRVNSGFPGLLILVWIISTVATIP-VLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKV--SRLIRIRLIVDDQ-DHTKVLGYLRNHSNLSKE--TSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQ-LNSLMILFMSFDNAYKATEV---LPYTLLLGYLHSSMSPIVYWFINSSFKQS 1014
L ++ +Y P + V I ++F++++IGN LV + K MR N ++NLSLAD+ VT+ C+PA L I W G+ +CK+ Y Q V ++ S L+L +++ R L+ KS + NS ++++WI+S + IP + +++ L N + T C E+W Y +Y+I + ++ PL +M++ Y I K+ ++ +V + + + R SK +++ A ++ R ++ ML++++LVFAIC++P LN L +F F + V ++ L Y +S+ +PI+Y F++ F++
Sbjct: 40 LRYLWREYLHP-KEYEWVLIAGYIIVFVVALIGNVLVCVAVWKNHHMRTVTNYFIVNLSLADVLVTITCLPATLVVDITETWFFGQSLCKVIPYLQTVSVSVSVLTLSCIALDRWYAICHPLMFKSTAKRARNS-----IVVIWIVSCIIMIPQAIVMERSSMLPGLANKT----TLFTVCDERWGGEVYPKMYHICFFLVTYMAPLCLMVLAYLQIFRKLWCRQIPGTSSVVQRKWKQPQPVSQPRGSGQQSKARISAVAAEIKQIRARRKTARMLMVVLLVFAICYLPISILNVLKRVFGMFTHTEDRETVYAWFTFSHWLVYANSAANPIIYNFLSGKFREE 374
BLAST of Orphan G-protein coupled receptor 22 vs. UniProt/SwissProt
Match: sp|P70031|CCKAR_XENLA (Cholecystokinin receptor OS=Xenopus laevis OX=8355 GN=cckar PE=2 SV=1) HSP 1 Score: 109.383 bits (272), Expect = 3.910e-25 Identity = 96/355 (27.04%), Postives = 173/355 (48.73%), Query Frame = 1
Query: 79 VYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKSYQNKRV---NSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPL-ETSIAY-CFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLG---------------------YLRNHSNLSKETS----------LNAHSREYRQKKFMVGMLIILILVFAICWMP-FQLNSLMILFMSFD--NAYKATEVLPYTL--LLGYLHSSMSPIVYWFINSSFKQSLI 1020
V I + S+IFLLSV GN L++I+ + KR+R N LL+L+L+DL V ++C+P L ++ +I GEV+C+ + Y G+ ++ S+ +L +SI R + RV S ++ W++S++ IP L + KT ++ P+ + + + C W + Q +Y+ +L I F +P VMI+ Y I ++ R I+ + ++ + G RN +S T +N + KK ++ MLI+++ +F ICWMP F N+ + +FD +A+ P + LL Y + ++P++Y F+N F+++ +
Sbjct: 70 VRIVMYSVIFLLSVFGNTLIIIVLVMNKRLRTITNSFLLSLALSDLMVAVLCMPFTLIPNLMENFIFGEVICRAAAYFMGLSVSVSTFNLVAISIERYSAICNPLKSRVWQTRSHAYRVIAATWVLSSIIMIPYLVYNKT--------VTFPMKDRRVGHQCRLVWPSKQVQQAWYVLLLTILFFIPGVVMIVAYGLISRELYRGIQFEMDLNKEAKAHKNGVSTPTTIPSGDEGDGCYIQVTKRRNTMEMSTLTPSVCTKMDRARINNSEAKLMAKKRVIRMLIVIVAMFFICWMPIFVANT----WKAFDELSAFNTLTGAPISFIHLLSYTSACVNPLIYCFMNKRFRKAFL 412
BLAST of Orphan G-protein coupled receptor 22 vs. UniProt/SwissProt
Match: sp|P58308|OX2R_MOUSE (Orexin receptor type 2 OS=Mus musculus OX=10090 GN=Hcrtr2 PE=2 SV=2) HSP 1 Score: 108.227 bits (269), Expect = 1.076e-24 Identity = 94/344 (27.33%), Postives = 169/344 (49.13%), Query Frame = 1
Query: 28 LDIIYSQYFQPLTRWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL------LVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKV--SRLIRIRLIVDDQ-DHTKVLGYLRNHSNLSKE--TSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQ-LNSLMILFMSFDNAYKATEV---LPYTLLLGYLHSSMSPIVYWFINSSFKQS 1014
L ++ +Y P + V I ++F++++IGN LV + K MR N ++NLSLAD+ VT+ C+PA L I W G+ +CK+ Y Q V ++ S L+L +++ R L+ KS + NS ++++WI+S + IP + + + L L T C E W Y +Y+I + ++ PL +MI+ Y I K+ ++ +V + + + R SK +++ A ++ R ++ ML++++LVFAIC++P LN L +F F + V ++ L Y +S+ +PI+Y F++ F++
Sbjct: 40 LRYLWREYLHP-KEYEWVLIAGYIIVFVVALIGNVLVCVAVWKNHHMRTVTNYFIVNLSLADVLVTITCLPATLVVDITETWFFGQSLCKVIPYLQTVSVSVSVLTLSCIALDRWYAICHPLMFKSTAKRARNS-----IVVIWIVSCIIMIPQAIVMECSSMLPGLANKTTLFT---VCDEHWGGEVYPKMYHICFFLVTYMAPLCLMILAYLQIFRKLWCRQIPGTSSVVQRKWKQQQPVSQPRGSGQQSKARISAVAAEIKQIRARRKTARMLMVVLLVFAICYLPISILNVLKRVFGMFTHTEDRETVYAWFTFSHWLVYANSAANPIIYNFLSGKFREE 374
BLAST of Orphan G-protein coupled receptor 22 vs. TrEMBL
Match: R7V1S8 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_117296 PE=3 SV=1) HSP 1 Score: 152.91 bits (385), Expect = 7.999e-40 Identity = 106/314 (33.76%), Postives = 161/314 (51.27%), Query Frame = 1
Query: 103 IFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKSYQNKRVNSGF-PGLLILV-WIISTVATIPVLYFRKTNRLGEYLNISLP-LETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYK--ATEVLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLL 1029
+F LS+ GN LV+ + K MR +N+ L+NL++ADL VTL+C+P + ++ W+ G MCK+ Y QGV + S +L +SI R L K+ NS PGLL L W+ + + P+L R+ + + + P I+YC E+W K + Y I++L + +ILPL ++I YS I K+ + D T +LG +K + MLI LI VF + W+P+ + SL S D YK VLPYTLLL + HS+++P +YWF+N SF+ + + L
Sbjct: 19 VFALSLAGNSLVIYVVYKNPHMRRLKNLFLVNLAIADLAVTLICMPLTAGQIVFRLWVYGGAMCKLIGYIQGVAVNCSIFTLAALSIDRYLAIKNSVVGSCNSCMSPGLLFLATWLPAAICMSPLLCVRQVHAM------AFPEFGIEISYCIEEWPKQMDRKGYGIALLFVTYILPLVIIIGCYSLIGRKLCS-VEFHRKTSDSSSTVMLG-----------------------RKRVARMLIALIAVFVLSWLPYNVTSL-----SLDLNYKYLDARVLPYTLLLAHAHSAVNPFLYWFLNKSFRHCMRRAL 297
BLAST of Orphan G-protein coupled receptor 22 vs. TrEMBL
Match: A0A0K0PUI1 (Orphan G-protein coupled receptor 22 OS=Platynereis dumerilii OX=6359 PE=2 SV=1) HSP 1 Score: 156.762 bits (395), Expect = 4.262e-39 Identity = 107/313 (34.19%), Postives = 169/313 (53.99%), Query Frame = 1
Query: 103 IFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKS-YQNKRVNSGFP--GLLILVWIISTVATIPVLYFRKTNRLG-EYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATE--VLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIK 1023
+FLLS+IGNGLV+ + + MR +N+ L+NL+ ADL VTL+C+P + + I W+ G VMCK+S Y QGV + +S +L +S+ R L K+ + R++S G L +WI+S V PVLY R + E+ I S+ C E W K Y+ Y I +L + + +P+ ++ + YS I R + D+ H K ++ S T + R R MLI+LI +F CW+P+ + SL S D + T+ +LP+TL L + HS+++P++YWF+N +F+ S+ K
Sbjct: 73 VFLLSLIGNGLVIFVVFRNAHMRRVKNLFLVNLAFADLLVTLICMPTIVGQIIVRLWVYGTVMCKVSAYMQGVAVLASIFTLTTLSVDRYLALKAPFSEHRMSSKKQALGCLATIWILSAVFLAPVLYVRNLKNVDFEHFGI------SVTVCIELWPKGIYRKSYAIFLLLLTYAIPILIISVCYSLIG---------RTLCSDELHRK--------TSDSSSTVMMGRKRVAR-------MLIVLIALFISCWLPYNIFSL-----SLDVSSHITDALILPFTLCLAHAHSAVNPVLYWFLNKNFRHSIRK 350
BLAST of Orphan G-protein coupled receptor 22 vs. TrEMBL
Match: V4BC87 (G_PROTEIN_RECEP_F1_2 domain-containing protein (Fragment) OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_102897 PE=4 SV=1) HSP 1 Score: 143.665 bits (361), Expect = 2.287e-36 Identity = 100/316 (31.65%), Postives = 174/316 (55.06%), Query Frame = 1
Query: 103 IFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKSYQ-NKRV-NSGFPGLLI-LVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVG------MLIILILVFAICWMPFQLNSLMILFMSFDNAYKATEVLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIK 1023
IFLL+V+GN V+++ L +R N+ L+NL++AD V +VCIP + +++Y+ WI GE MCK++ + QGV + +S ++ +S+ RLL + KRV N +I ++WI+S P+L +RKT L L S+ +C E W +++ IY + AI +++P ++ +Y LI +L +D++ R+ S +S+ S +K MVG MLI L ++FA CW+P+ + +L + F + D + VLP+T+ LG+ +S+++PI+Y++ + SF++ L K
Sbjct: 8 IFLLAVLGNSSVLMIILWHPPLRTITNLFLVNLAMADFSVCIVCIPMAVGQAVYSVWIYGEFMCKVTGFLQGVTVVASIFTIAVLSLDRLLAIRHPMIFKRVANYKMAARIISVIWILSCSVMAPLLVYRKTQTL------PLSPNESLHFCHEDWPSLKHRQIYDSCLFAIVYVIPGCIIATSYG--------LIGRQLWTEDENLK------RSESEISRGMS----------QKIMVGRKRIAKMLIALAILFAACWLPYYIVTLYLDFET-DQSDDFLLVLPFTIFLGHANSALNPILYFYTSQSFRKYLFK 292
BLAST of Orphan G-protein coupled receptor 22 vs. TrEMBL
Match: A0A210Q9G0 (Pyroglutamylated RFamide peptide receptor OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT08061 PE=3 SV=1) HSP 1 Score: 145.206 bits (365), Expect = 1.531e-35 Identity = 102/317 (32.18%), Postives = 178/317 (56.15%), Query Frame = 1
Query: 103 IFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTK---SYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQ---DHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMP-FQLNSLMILFMSFDNAYKATEVL-PYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLL 1029
+FL +VIGN V+++ + +RM N LLNL++ADL VT VCIP L + IY WI GE++CK++ Y QGV +T+S+L++ MSI R L + ++N R ++I++W +S + ++P+L+ R T ++ + L T + YC E+W + +Y +++L I I+P +++ Y L+ RL V D+ D T+ G + ++ +E SL +R R+ + + I + +VF CW P F +N+ ++ F +A E L PY LL+G++H +PI+Y F++ SF+ +++ +
Sbjct: 60 VFLTAVIGNIAVLLVIIPNRRMWSVTNNFLLNLAIADLLVTFVCIPMTLAQKIYPLWIYGEMLCKLTPYLQGVSVTASTLTITTMSIDRCLAIRHPMKFRNVRTTKYVRIVIIVIWCVSFLLSVPILFVRIT------VSHDILLGTVMTYCMEEWVNIASRQVYDVTLLFIICIIPGTTILVAYL--------LMGKRLWVPDRKLSDDTRKSGRGK-RTDSERERSLRNITRNRRR---LAKLCITIAIVFIACWTPYFVINT----YLDFSMDVRAAERLSPYMLLIGHVHCVTNPILYCFLHKSFRHYVMRCI 354
BLAST of Orphan G-protein coupled receptor 22 vs. TrEMBL
Match: V4ALY3 (G_PROTEIN_RECEP_F1_2 domain-containing protein (Fragment) OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_71923 PE=3 SV=1) HSP 1 Score: 141.354 bits (355), Expect = 1.711e-35 Identity = 94/317 (29.65%), Postives = 175/317 (55.21%), Query Frame = 1
Query: 103 IFLLSVIGNGLVVILALKFKRMRV-SQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKSYQNKR---VNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETS-LNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATEVLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLLGQQ 1038
IFL++VIGN V+I+ L ++MR S N L+N+++AD+ V + CIP + +Y WI GE MCK++ Y QG+ + +S L++ MS+ R + + R L++ W+++ +P++ RK + +N + SI+YC E+W EY+ IY + + +I+P +++ Y+ LI RL V D ++LS++ S +N + ++ + M+II+ ++FA+CW+P+ + +L+++F S + K + P+ LLLG+ +S+ +PI+Y F++ FK+ + +L+ Q
Sbjct: 7 IFLMAVIGNIFVLIVILLNQKMRSNSANYFLVNMAIADILVAVCCIPMTVAEIVYQVWIFGEFMCKVTGYLQGIYVGASVLTIVCMSVDRFVAIRYPMKSRQIFTVKKTKQLILTTWLLAIGVMVPLILVRKLDDYNLVVN-----DLSISYCVEQWPSTEYRQIYDVFLFCFIYIIPGVAVMLLYA--------LIGCRLWVKD-------------TSLSRQNSYMNNEVKIMMSRRRLALMMIIISVLFAVCWLPYYILNLLMVF-SPRISRKVIPLYPFALLLGHSNSAQNPILYCFMHRKFKECIFRLVKCQ 296
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Cavefish
Match: npffr1 (neuropeptide FF receptor 1 [Source:ZFIN;Acc:ZDB-GENE-030131-8106]) HSP 1 Score: 114.775 bits (286), Expect = 3.630e-28 Identity = 87/347 (25.07%), Postives = 168/347 (48.41%), Query Frame = 1
Query: 10 NNSAVVLDIIYSQYFQPLTRWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL-LVTKSYQNKRVNSGFPGLLILVWIISTVATIP-----VLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYK------ATEVLPYTLLLGYLHSSMSPIVYWFINSSFKQS 1014
N++ ++L+ +S Y+Q V+I LIF L ++GN LV ++ LK K+MR NI +LNL+++DL V + C+P L ++ T W EV CK+S QG +++S +L +++ R + +Q K + +W ++ + P + + + + N + PL T C+E W + IY + + + ++ P+ +++I Y+ I K+ ++ +++ + + R YR+K +V ML+I+ L+F + W+P ++IL + N A + P+ L + +SS++PIVY + N +F++
Sbjct: 24 NDTDILLNYTFSSYYQHSPPVATVFILAYILIFFLCMVGNLLVCLIVLKNKQMRTVTNIFILNLAISDLLVGIFCLPITLADNLVTGWPFDEVTCKMSGLIQGASVSASVFTLVAIAVERFRCIVYPFQQKLTLRQAIITIAFIWALAIIIMCPSAVTLTVSHDAFHFMVDRDNATYPLYT----CWEAWPDQSMRKIYTMVLFSHIYLAPVTLIVIMYTRIALKL---------------------FKSPASIRDAQAEDEGRRVYRRKIRVVNMLLIVALLFTVSWLPLW---IIILLTDYGNMSSSQLNVIAVYIFPFAHWLAFFNSSINPIVYGYFNENFRRG 342
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Cavefish
Match: QRFPR (pyroglutamylated RFamide peptide receptor-like [Source:NCBI gene;Acc:103023541]) HSP 1 Score: 109.383 bits (272), Expect = 4.109e-26 Identity = 88/332 (26.51%), Postives = 164/332 (49.40%), Query Frame = 1
Query: 64 TRWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISR-------LLVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATE---VLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLL 1029
R R ++ + IF L+++GNG+V+ + + R + ++ + +L+L+DL +T C+P L ++I + W G ++CK + Q I + L++ +++ R L + + Y KR +L LVW++S + P+L+ ++ ++L E C E W ++ IY ++ F+LPL M+ Y+ ++S + IR V D VL + NH +SK + R+KK V M+I ++L+F ICW PF ++ + ++ Y ++ +G+ +S +PIVY F+N +FK+S + L
Sbjct: 107 ARARTAFVLTYAAIFALALLGNGVVLCVLAREGAARRASSLFMCSLALSDLLITFFCVPFTLLQNISSQWSGGVLVCKTVPFVQTTAIVTGILTMTCIAVERYQGIVHPLKMKRQYTPKRAYK----MLGLVWVVSIMVGSPMLFVQQLEVKYDFL-----YEHHHVCCQELWGSELHRKIYTTFIMVALFLLPLAAMLFLYT----RISIELWIRKRVGD---VSVLSTM-NHREISKIS---------RKKKRAVKMMITIVLMFTICWAPFHTVHMLFEYNDLESKYDDVTLNMIIAVVQAIGFSNSFNNPIVYAFMNENFKKSCVSTL 412
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Cavefish
Match: QRFPR (pyroglutamylated RFamide peptide receptor-like [Source:NCBI gene;Acc:103023541]) HSP 1 Score: 109.383 bits (272), Expect = 4.109e-26 Identity = 88/332 (26.51%), Postives = 164/332 (49.40%), Query Frame = 1
Query: 64 TRWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISR-------LLVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATE---VLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLL 1029
R R ++ + IF L+++GNG+V+ + + R + ++ + +L+L+DL +T C+P L ++I + W G ++CK + Q I + L++ +++ R L + + Y KR +L LVW++S + P+L+ ++ ++L E C E W ++ IY ++ F+LPL M+ Y+ ++S + IR V D VL + NH +SK + R+KK V M+I ++L+F ICW PF ++ + ++ Y ++ +G+ +S +PIVY F+N +FK+S + L
Sbjct: 107 ARARTAFVLTYAAIFALALLGNGVVLCVLAREGAARRASSLFMCSLALSDLLITFFCVPFTLLQNISSQWSGGVLVCKTVPFVQTTAIVTGILTMTCIAVERYQGIVHPLKMKRQYTPKRAYK----MLGLVWVVSIMVGSPMLFVQQLEVKYDFL-----YEHHHVCCQELWGSELHRKIYTTFIMVALFLLPLAAMLFLYT----RISIELWIRKRVGD---VSVLSTM-NHREISKIS---------RKKKRAVKMMITIVLMFTICWAPFHTVHMLFEYNDLESKYDDVTLNMIIAVVQAIGFSNSFNNPIVYAFMNENFKKSCVSTL 412
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Cavefish
Match: npffr1 (neuropeptide FF receptor 1 [Source:ZFIN;Acc:ZDB-GENE-030131-8106]) HSP 1 Score: 108.612 bits (270), Expect = 4.473e-26 Identity = 87/347 (25.07%), Postives = 163/347 (46.97%), Query Frame = 1
Query: 10 NNSAVVLDIIYSQYFQPLTRWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL-LVTKSYQNKRVNSGFPGLLILVWIISTVATIP-----VLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYK------ATEVLPYTLLLGYLHSSMSPIVYWFINSSFKQS 1014
N++ ++L+ +S Y+Q V+I LIF L ++GN LV ++ LK K+MR NI +LNL+++DL V + C+P L ++ T W EV CK+S QG +++S +L +++ R + +Q K + +W ++ + P + + + + N + PL T C+E W + IY + + + ++ P+ +++I Y+ I K+ + + R YR+K +V ML+I+ L+F + W+P ++IL + N A + P+ L + +SS++PIVY + N +F++
Sbjct: 14 NDTDILLNYTFSSYYQHSPPVATVFILAYILIFFLCMVGNLLVCLIVLKNKQMRTVTNIFILNLAISDLLVGIFCLPITLADNLVTGWPFDEVTCKMSGLIQGASVSASVFTLVAIAVERFRCIVYPFQQKLTLRQAIITIAFIWALAIIIMCPSAVTLTVSHDAFHFMVDRDNATYPLYT----CWEAWPDQSMRKIYTMVLFSHIYLAPVTLIVIMYTRIALKLFK--------------------------------SPGRRVYRRKIRVVNMLLIVALLFTVSWLPLW---IIILLTDYGNMSSSQLNVIAVYIFPFAHWLAFFNSSINPIVYGYFNENFRRG 321
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Cavefish
Match: hcrtr2 (hypocretin receptor 2 [Source:NCBI gene;Acc:103037678]) HSP 1 Score: 107.071 bits (266), Expect = 2.072e-25 Identity = 94/352 (26.70%), Postives = 168/352 (47.73%), Query Frame = 1
Query: 4 RFNNSAVVLDIIYSQYFQPLTRWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL-LVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSI-AYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKV---------SRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQ-LNSLMILFMSFDNAYKATEV---LPYTLLLGYLHSSMSPIVYWFINSSFKQS 1014
+ ++ +L I+ +Y P ++ V I +FLLS+IGN LV K MR N ++NLS AD+ VT+ C+PA L I W LG+++C+I Y Q + ++ S L+L ++ R + + K +IL+W++S V IP + + L L L +T + C E W Y IY+ + + PL +M++ Y I K+ S L R + H+ G S + ++++A ++ + ++ ML++++ VFA+C++P LN + +F +F N V ++ L Y +S+ +PI+Y F++ F++
Sbjct: 31 QLDDDEELLKYIWREYLHP-KQYEWVLIAGYIAVFLLSLIGNALVCFAVWKNHHMRTVTNYFIVNLSFADILVTITCLPASLVVDITETWFLGDMLCRILPYFQTISVSVSVLTLSCIAQDRWYAICHPLKFKSTAKRARKSIILIWLVSCVIMIPQAVVMECSSLLPELLPELTNKTILFTVCDEHWGDEIYPKIYHTCFFIVTYFAPLCLMVLAYIQICHKLWCQQIPGTSSALQRKWKSLQCSAHSAGPG----ESVKVRTSAVSAEIKQVKARRKTARMLMVVLFVFALCYLPISVLNIMKRVFGAFKNTNNRETVYAWFTFSHWLIYANSAANPIIYNFLSGKFREE 377
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Sea Lamprey
Match: qrfpr4 (pyroglutamylated RFamide peptide receptor 4 [Source:ZFIN;Acc:ZDB-GENE-091204-354]) HSP 1 Score: 120.168 bits (300), Expect = 3.069e-31 Identity = 95/332 (28.61%), Postives = 164/332 (49.40%), Query Frame = 1
Query: 55 QPLTRWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL------LVTKS-YQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQ-LNSLMI---LFMSFDNAYKATEVLPYTLLLGYLHSSMSPIVYWFINSSFKQSL 1017
QPL RL + + +L+F L++ GN LV+ + + K + N+ + +L+++DL +T+ CIP L ++ ++ WI G +CK+ + Q +T+S L++ +++ R L ++S Y R S G VW++ P+ Y + L + S C E+W E++ Y ++L + F+LPL M Y+ + + L V + H V L+ S ++K T R KK V M++I++++FA+CW PF +NSL+ L MSFD L L G+ +S +PIVY +N ++K+ L
Sbjct: 42 QPL---RLAFAVVGALVFTLALSGNALVIFVVARRKALHTVANVFVCSLAVSDLMITVFCIPITLMQNFFSNWIAGVALCKLVPFIQVTAVTTSMLTMACIAVERFQGILHPLRSRSRYTTARAASMLCG----VWLVGLCVAAPMFYAHTVQVTYDVL-----YDVSHTGCSEQWSHAEHRQAYTTTLLLVTFVLPLLTMTALYTRLAHE--------LWVKTRVHDAVFNALQR-SEINKIT---------RSKKRAVKMMVIVVILFAVCWAPFHVVNSLVPKGNLEMSFD---MEAITLAMVQLFGFSNSLWNPIVYAALNQNYKKQL 340
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Sea Lamprey
Match: kiss1rb (KISS1 receptor b [Source:ZFIN;Acc:ZDB-GENE-060526-54]) HSP 1 Score: 100.523 bits (249), Expect = 3.295e-24 Identity = 86/319 (26.96%), Postives = 151/319 (47.34%), Query Frame = 1
Query: 97 SLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKSYQNKRVNSGFPGLL----ILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYL--RNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDN----AYKATEVLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIK 1023
+LI L+ ++GN LV+ + K ++MR N ++NL+ D+ + C+P W+ G+ MCK Y Q V + ++ ++L MS+ R VT Y + + P L I +WI S V ++P+ + KT+ Y YCF+ + ++ + + + ++LPL + + YS ++ K+ R V+ QD L L RN + +K T M+++++LVF +CW P Q L ILF SF +Y ++ + + Y +SS++P VY F+ SF+++ K
Sbjct: 44 ALIMLVGLVGNSLVIFVITKNRQMRTVTNFFIVNLAGTDIIFLVCCVPFTATLYPLPSWVFGDFMCKFVNYLQQVTVQATCMTLTAMSVDRCYVT-VYPIRSLQHRTPCLAACISIGIWIGSLVMSLPMALYHKTDTKEWY--------GPKTYCFDAFPSVPHEHAFVLYSFVLVYMLPLVTISVCYSIMIKKIG-----RPAVEPQDDNVQLQMLTERNEATRAKVTR----------------MVVVVVLVFMLCWGPIQ---LFILFQSFYPHFAISYTTYKLKTWANCMSYANSSINPFVYCFMGDSFRKTFKK 329
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Sea Lamprey
Match: npffr1l3 (neuropeptide FF receptor 1 like 3 [Source:ZFIN;Acc:ZDB-GENE-070424-44]) HSP 1 Score: 96.2857 bits (238), Expect = 1.212e-22 Identity = 84/324 (25.93%), Postives = 155/324 (47.84%), Query Frame = 1
Query: 79 VYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL-LVTKSYQNK-RVNSGFPGLLILVWIISTVATIP-VLYFRKTNRLGEYL------NISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMS---FDNAYKATEVLPYTLLLGYLHSSMSPIVYWFINSSFKQS 1014
VY+ IFLL +IGNGLV + L+ +RMR N+ +LNL+++DL V + C+P L ++ T W G V+CK+S QG +++S +L +++ R + ++ K R++ +L+ +W ++ P L G Y+ N + PL C+E W + Y + A ++ PL ++++ Y+ I F++ L S+ A R+ K ++ MLII+ ++F + W+P +++ + + +D A+ V P L + +SS++PIVY + N +F++
Sbjct: 51 VYLIAYLFIFLLCMIGNGLVCYVVLQSRRMRTVTNLFILNLAVSDLLVGIFCMPITLVDNLITGWPFGVVVCKMSGLVQGTSVSASVFTLVAIAVDRFRCIVYPFKRKLRISHALFTILV-IWALALSIMCPSALMLTAEVSRGHYMVYSDGSNDTHPLHQ----CWEAWPDPRMRKAYTTVLFANVYLAPLALILLMYARIGFRLCSLSASAAAAASAAAP------------SRGGGGGATGVTRRRVK-VIKMLIIVAMLFMLSWLPLWTVMMLVDYANLSPWDLAFLNMYVYPVAHWLAFFNSSVNPIVYGYFNENFRRG 356
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Sea Lamprey
Match: npy8br (neuropeptide Y receptor Y8b [Source:ZFIN;Acc:ZDB-GENE-980526-208]) HSP 1 Score: 86.2705 bits (212), Expect = 4.094e-19 Identity = 85/341 (24.93%), Postives = 150/341 (43.99%), Query Frame = 1
Query: 28 LDIIYSQYFQPLTRWRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISR--LLVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNIS--LPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPF-------QLNSLMILFMSFDNAYKATEVLPYTLLLGYLHSSMSPIVYWFINSSFKQSL 1017
LD+ + Q Q + + V T C LI L ++GN L+V++ L+ + NI ++NL+ +D+ V LVC+P + ++ +WI GE +CK S + Q ++ S SL ++I R L++ + +N + + +W+ + + P L F Y N+S P C E W +F + S+L F PL + + Y +R+ + + K+L +E N + +K M ML+ ++ FAICW+P N ++L D + L L ++PI Y F+N++F++ L
Sbjct: 26 LDLDHCQTPQAVRSF--VIATYCVLI-ALGLLGNSLLVLVILRHSELHNVTNILIVNLAFSDMLVGLVCLPLTIAYTLMDHWIFGEALCKGSPFLQCSAVSVSIFSLVLIAIERHQLIINPTGWKPSLNHAYVAIGA-IWVTAFAMSSPFLAFHVLTD-EPYRNLSHYFPDYGEKVACIELWPSDTSKFAFTTSLLVFQFSCPLLFVFLCY------------LRIFLRLRQRKKMLP-------TGREGGGNGVRASHMKKINM--MLVAIVAGFAICWLPLYAFNAVSDWNPTLLLHCQHDLIFSLCH------LTAMLSICINPIFYGFLNNNFQKEL 334
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Sea Lamprey
Match: si:dkey-247m21.3 (si:dkey-247m21.3 [Source:ZFIN;Acc:ZDB-GENE-091204-249]) HSP 1 Score: 83.1889 bits (204), Expect = 4.801e-18 Identity = 75/323 (23.22%), Postives = 152/323 (47.06%), Query Frame = 1
Query: 97 SLIFLLSVIGNGLVVILALKFKRMR-VSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL--LVTKS--YQNKRVNSGFPGLLILVWII-STVATIPVLYFRKTNRLGEYLNISL--PLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLR----NHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATEVLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLL 1029
S+I ++++GN LVV+ K + +R + N +++L++ADL V ++ +P + W G+ C I + T+S + L +++ R + K YQNK +L W++ S ++ IP+ + + L + L E + +C F +Y ++ + F +PL +M Y R+ V + H + +G L+ + + T + S +R + L I++ F +CW PF +N+++ F+++D + + L LGY +S+++P++Y F+N SF+++ + +L
Sbjct: 27 SIIIAVTILGNLLVVVAVCKDRHLRKIKTNYFIVSLAVADLLVAILVMPFGAVERVQNDWPYGDKFCIIRTSFDVMFTTASIMHLCCIALDRFYAICCKPLVYQNKMTPLRVGLMLGACWVVPSLISFIPIANGWNIIGIEQQLELRLGQAKEANQTHCI-----FMVNKVYAVACSLVAFYIPLGLMAAAYQ------------RIYVTARAHARQIGSLQRAVSKDTTGDEATMVQTQSTRFRHETKAAKTLAIIMGCFCLCWAPFFINNVIDPFVNYDTPQQLWDAW---LWLGYANSALNPLLYAFLNRSFRRAFLAIL 329
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Nematostella
Match: EDO27481 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T950]) HSP 1 Score: 117.857 bits (294), Expect = 1.095e-30 Identity = 95/321 (29.60%), Postives = 161/321 (50.16%), Query Frame = 1
Query: 73 RLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTY-WILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKSYQNKRVN-SGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKA----TEVLPYTLLLGYLHSSMSPIVYWFINSSFKQSL 1017
R + +TL +LIFL+S GN LV I+ LK K+M+ N +LNL++ADL +T +CIP ++ Y W G VMCK+ Y Q + + +S +L +S++R K+++ S L+I +W++S + P Y+N SL YC E WD + Y S+ A +++PL ++ Y I ++SR V +Q H KET K +V ML+I+ L+FA+C +P N++M L++ F A K+ +V+ + + +L+S+ +PI Y +N ++++ +
Sbjct: 3 RTIKLTLYTLIFLVSAAGNTLVCIVILKRKKMKTVTNYFILNLAVADLALTCICIPFDIPVQEMGYIWPYGAVMCKVLYPLQTLTLFASVYTLTAVSLTRYWAIVHPLRKQLSVSKAKTLVIAIWVLSLLPVAP------------YMN-SLSYVPHSKYCEENWDNHNARKGYTASLFAFQYVIPLTIIATAYCSITSELSRRTIESACVRNQQH--------------KET------------KKVVRMLMIVTLLFAVCVLP---NNVMWLWLDFGAADKSFKYFWDVVAFCHVWTFLNSAANPICYTILNENYRKEI 281
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Nematostella
Match: EDO36453 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIH9]) HSP 1 Score: 118.242 bits (295), Expect = 1.419e-30 Identity = 92/321 (28.66%), Postives = 160/321 (49.84%), Query Frame = 1
Query: 73 RLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTY-WILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKSYQNKRVN-SGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKA----TEVLPYTLLLGYLHSSMSPIVYWFINSSFKQSL 1017
R + +TL +LIFL+S GN LV I+ LK K+M+ N +LNL++ADL +T +CIP ++ Y W G VMCK+ Y Q + + +S +L +S++R K+++ S L+I +W++S + P Y+N SL YC E WD + Y S+ A +++PL ++ Y I ++SR V +Q H ++ K +V ML+I+ L+FA+C +P N++M L++ F A K+ +V+ + + +L+S+ +PI Y +N ++++ +
Sbjct: 35 RTIKLTLYTLIFLVSAAGNTLVCIVILKRKKMKTVTNYFILNLAVADLALTCICIPFDIPVQEMGYIWPYGAVMCKVLYPLQTLTLFASVYTLTAVSLTRYWAIVHPLRKQLSVSKAKTLVIAIWVLSLLPVAP------------YMN-SLSYVPHSKYCEENWDNHNARKGYTASLFAFQYVIPLTIIATAYCSITRELSRRTIESACVRNQQH--------------------------KETKKVVRMLMIVTLLFAVCVLP---NNVMWLWLDFGAADKSFKYFWDVVAFCHVWTFLNSAANPICYTILNENYRKEI 313
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Nematostella
Match: EDO49619 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFL1]) HSP 1 Score: 97.8265 bits (242), Expect = 2.427e-23 Identity = 76/313 (24.28%), Postives = 149/313 (47.60%), Query Frame = 1
Query: 97 SLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIY-TYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL-LVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATEVLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLL 1029
++IF++ V+GN LV ++ K MR ++N ++NL++AD+ V LVCIP ++ +Y W+LG MCK+ + + + T S+ +L +S R V ++ + +++ W++S + +P + L L C E W K ++ +Y + + + LPL ++ YS + K LR SN ++ L + + ++ ML+ ++L++A+C +P Q+ L + F + + +L +T +L Y++S ++P +Y +N FK+ +LL
Sbjct: 3 AIIFIVGVVGNSLVCLVIFKTPNMRNTRNYLIVNLAIADITVALVCIPFDVVIRVYGDTWVLGAAMCKMVWPSMTFVATCSAATLAAISFDRYRAVVHPWKPRLSMRQTLAVIVSTWLVSFLVVLPYI---------------LVLGMDGHSCTEFWLKDIHRQLYTVFLFVFAYALPLVIIAFAYSRVALK----------------------LREQSNRIEKNKLASGIPSSSKYNWITKMLVTVVLIYALCLLPNQVLWLWMEFGTGNEFEHMNTLLTFTGMLVYINSIVNPFLYAGLNDEFKKGFARLL 278
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Nematostella
Match: EDO36473 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIH8]) HSP 1 Score: 94.3597 bits (233), Expect = 4.353e-22 Identity = 81/320 (25.31%), Postives = 155/320 (48.44%), Query Frame = 1
Query: 73 RLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTY-WILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKSYQNKRVNSGFPGLLI-LVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKAT----EVLPYTLLLGYLHSSMSPIVYWFINSSFKQS 1014
R++ + L + IFL+S +GN +V + ++ K+M+ N +LNL++ADL +T +CIP ++ Y W G VMCK+ Y Q + + +S +L + ++R ++++ L+I +W+ S V P Y N SL C E W + + I+ + ++LPL V+ Y+ I R +R R +H LR + ++E R+ +V ML ++ +VFAIC +P N++M L++ F +A + E++ + ++ + +S+ +P+ Y +N ++++
Sbjct: 7 RIIKLVLYAAIFLISAVGNTMVCAIIVRRKKMKTVTNYFILNLAVADLALTCICIPFDIPVQEMGYIWPYGAVMCKVLYPLQTLTLFASVYTLTAVGLTRYWAIVHPMKRQLSITQSKLVIAFIWLGSVVPVFP------------YAN-SLKFTRETHTCEEYWSSKKVREIFTAVIFVFQYVLPLGVIGTAYAAI----GRELRRRKATKSGNHK-----LR-----------HLQAQEARK---VVNMLSVVTMVFAICVLP---NNIMWLWLDFGDADQKVEYFWEIVAFCNVVTFANSAANPVCYTALNETYRRE 287
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Nematostella
Match: EDO46084 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR06]) HSP 1 Score: 93.9745 bits (232), Expect = 1.364e-21 Identity = 87/320 (27.19%), Postives = 161/320 (50.31%), Query Frame = 1
Query: 70 WRLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTY-WILGEVMCKISYYAQGVMITXXXXXXFGMSISRL-LVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKAT---EVLPYTLLLGYLHSSMSPIVYWFINSSFKQS 1014
++++ ++L +LIFL+S +GN LV I+ + +RMR N +LNL++ADL +TL+CIP ++ Y W G MCKI Y Q + + +S +L +S++R + + + S ++IL+W IS+ T+P Y+ + L L+ C E W + Y+ Y + + ++LPL ++ Y I F++ + + R + ++L +E +K +V MLII+ L+FA+C +P N++M L++ F N E++ T ++ + +S+ +P+ Y N F++
Sbjct: 34 FKVLKLSLYALIFLISTVGNSLVCIVIARRRRMRTVTNYFILNLAIADLAITLICIPFDIPVQENGYKWPYGAFMCKIMYPLQTMCMFASIFTLVAVSLNRFWAIVYPLRQEMSKSQATIIIILIWFISSGLTVP------------YVTV-LELKNGDD-CSESWPEPGYRKAYTACIFVVQYLLPLTLIAFAYLKIGFELRKCVGTR---------------------TANSALERAKQEDTRK--VVRMLIIVTLLFAMCVLP---NNVMWLWLDFGNGNDYEHIWEMVAVTNIILFANSATNPVAYTICNERFREE 313
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Medaka
Match: npffr2b (neuropeptide FF receptor 2-like [Source:NCBI gene;Acc:101174760]) HSP 1 Score: 111.694 bits (278), Expect = 5.351e-27 Identity = 85/317 (26.81%), Postives = 159/317 (50.16%), Query Frame = 1
Query: 100 LIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL-LVTKSYQNKRVNSGFPGLLILVWIISTVATIPV-LYFRKTNRLGEYLNISLPLETSIAY-CFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATE---------VLPYTLLLGYLHSSMSPIVYWFINSSFKQS 1014
LIF++ ++GNG+V + L+ K MR N+ +LNL+++DL V + C+P L +I T W G ++CK+S QG+ +++S +L +++ R + ++ K +++++W+++ P + + T + + +T Y C E W E + IY + A ++ PL +++I Y+ I F L T+ G S+ + + S++ R+KK ++ ML+I+ L+F + W+P L L M D A + TE V P+ L + +SS++PI+Y F N +F++
Sbjct: 54 LIFVVCMVGNGVVCFIVLRSKNMRTVTNLFILNLAISDLLVGIFCMPTTLLDNIITGWPFGSIVCKLSGMVQGISVSASVFTLVAIAVDRFRCIVYPFRQKLTIGTSKMIIVIIWVLALFIMCPSGVMLQVTEEPTVRIVVDHRSKTRPFYWCRENWPSQEMRKIYTTVLFANIYLAPLNLIVIMYARIGFT--------LFKTTIPPTRGSGSATGESSSANKLSMDGRQTISRKKKRVIMMLVIVALLFVLSWLP-----LWTLMMLSDYA-RLTEHQYRVINIYVYPFAHWLAFFNSSVNPIIYGFFNENFRRG 356
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Medaka
Match: npy2r (neuropeptide Y receptor Y2 [Source:ZFIN;Acc:ZDB-GENE-040924-8]) HSP 1 Score: 106.301 bits (264), Expect = 2.005e-25 Identity = 84/314 (26.75%), Postives = 152/314 (48.41%), Query Frame = 1
Query: 97 SLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKSYQNKRVNSGFPGLLIL-VWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQF-IYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNA--YKATEVLPYTL--LLGYLHSSMSPIVYWFINSSFKQSLI 1020
S I L V GN LVV + KF+ +R N ++NL++ADL V +C+P L ++Y W+ G+V+C + +AQG+ + S+++L +++ R + +++ ++I+ W+IS + P+ FR EY + E SI C EKW +Y +SML + +PL + + Y IRI L++H + N H R + K M++ +++VFA+CW+PF L I DN + L +T+ +L + ++PI+Y ++N++++ + +
Sbjct: 55 STIILFGVTGNSLVVYVVYKFRNLRTVTNFFIINLAVADLLVNTLCLPFTLIYTLYDEWMFGQVLCYLLPFAQGMTVQVSTITLNIIALDRHRSIVHHMETKMSKDMCAMVIVFSWVISALLASPLAIFR------EYGSFDFSPEESIQVCTEKWPGSSMNGRLYSMSMLVVQVAIPLAINSVAY----------IRI------------WKKLKHHMKCGR----NDHQRRRKTTK----MMLTMVVVFAVCWLPFHAFQLAI---DIDNTVLHMKDFKLVFTVFHILAMCSTCVNPILYGWMNNNYRTAFL 329
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Medaka
Match: ENSORLT00000022492.2 (cholecystokinin receptor-like [Source:NCBI gene;Acc:101161683]) HSP 1 Score: 106.301 bits (264), Expect = 4.013e-25 Identity = 94/347 (27.09%), Postives = 160/347 (46.11%), Query Frame = 1
Query: 97 SLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKSYQNKRV---NSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRL-------------------IVDDQDHTKVLGYLRNHSN------------LSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMP-FQLNSLMILFMSFDN--AYKATEVLP--YTLLLGYLHSSMSPIVYWFINSSFKQSLI 1020
SLIF LSV GN L++++ KRMR N LL+L+++DL + + C+P L +I +I G MCKI Y G+ ++ S+ SL ++I R + RV S ++ W+++ + +P N T+ C KW + +Y+ +L I F +P VMII Y I ++ R IR + + DD D V R HS + N + KK ++ ML++++++F +CWMP + +N+ + +FD+ A +A P + LL Y + ++PI+Y F+N+ F+++L+
Sbjct: 67 SLIFFLSVFGNLLIIVVLTVNKRMRTVTNTFLLSLAVSDLMMAIFCMPFTLIPNILKDFIFGAAMCKIVSYLMGISVSISTFSLVAIAIERYSAICNPLKSRVWQTRSHAYRVIAATWVLAFIIMVPYPIISHLEPFQGSAN------TTAHKCRHKWPIQTAEQTWYVMLLLILFAIPGVVMIIAYGLISRELYRGIRFEMGQKKDSTDVKNGLTSTVSNVSDDGDGCYVTVVQRPHSMEMSTMAASIRQVKADRPRSNTSEAKLEAKKRVIRMLVVIVVLFFLCWMPLYCVNT----WRAFDDSSATRALSGAPIAFIHLLSYTSACVNPIIYCFMNTRFRKALL 403
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Medaka
Match: CCKAR (cholecystokinin A receptor [Source:NCBI gene;Acc:101166993]) HSP 1 Score: 105.145 bits (261), Expect = 1.257e-24 Identity = 93/355 (26.20%), Postives = 168/355 (47.32%), Query Frame = 1
Query: 73 RLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRL------LVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYF---RKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLI--VDDQDHTKVLGYLRNHSN------LSKETSL---------------------NAHSREYRQKKFMVGMLIILILVFAICWMP-FQLNSLMILFMSFDNAYKATEVLPYTLLLGYLHSSMSPIVYWFINSSFKQSLI 1020
R V I L LIFLLS++GN L++ + ++ +RMR N+ LL+L+++DL V+LVCIP L ++ +I G MCK+ Y GV ++ S+ +L +S+ R L ++ +Q K S ++ W+ S + +P F N + + +P +TS F+ W Y+S+L + F++P +M+ Y I ++ R I+ L +D G ++ + SK SL ++ KK ++ ML++++ +F +CW P F +N+ M + + + + LL Y + ++PI+Y F+N F+Q ++
Sbjct: 46 RTVRIFLYCLIFLLSLLGNSLIIAVLVRNRRMRTVTNLFLLSLAVSDLMVSLVCIPFTLIPNLMKNFIFGIGMCKLVMYFMGVAVSVSTFNLVAISLERYSAICNPLTSRVWQTK---SHAAKVITTTWVASFILMLPYAIFSTVESNNSPIKMCRLVMPNDTS----FQSW---------YVSLLLVLFLIPGIIMMTAYGLISLELYRGIKYELPKRKSSRDRQSSTGSIKPGDSDGCYLQPSKRKSLTLGSGSFGSSSGKQAVGRVSCSSSVSNLMAKKRVIRMLLVIVFLFFLCWTPIFVVNAWKAFDMESAHRHLTGAPISFIHLLSYTSACVNPIIYCFMNKRFRQGML 384
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Medaka
Match: QRFPR (pyroglutamylated RFamide peptide receptor [Source:NCBI gene;Acc:101157401]) HSP 1 Score: 101.679 bits (252), Expect = 1.372e-23 Identity = 83/331 (25.08%), Postives = 157/331 (47.43%), Query Frame = 1
Query: 73 RLVYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISR-------LLVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATE---VLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLLGQ 1035
++ ++ + LIF+L++ GN LV+ + +K + ++ + +I + +L+++DL +T CIP L ++I + W G ++CK + Q I + L++ ++I R L + + Y +KR +L LVWI S V P+L+ ++ L + C E W Y+ Y ++ F+LPL M+ Y+ I + + IR V D VL + NH ++ V M++ ++L+F ICW PF ++ + + Y ++ +G+ +S +PIVY F+N +FK++ + L +
Sbjct: 54 KITFVIMYVLIFVLALAGNTLVIYIVVKKRAIQTATDIFICSLAVSDLLITFFCIPFTLLQNISSEWFGGVLVCKTVPFVQTTAIVTGILTMTCIAIERYQGIVFPLKMRRQYSSKRAYK----VLGLVWIASVVVGSPMLFVQQ-------LQYDFLYDHYHVCCQESWSSLTYRQAYTTFIMVALFLLPLTAMLFLYTRIGIE----LWIRKRVGD---ASVLNTM-NH------------------REIAVKMMVTIVLLFTICWAPFHTVHMLFEYNDLEKKYDGVTLNMIIAIVQAVGFFNSFNNPIVYAFMNENFKKTCVSTLSR 347
BLAST of Orphan G-protein coupled receptor 22 vs. Planmine SMEST
Match: SMESG000008693.1 (SMESG000008693.1) HSP 1 Score: 399.438 bits (1025), Expect = 3.533e-141 Identity = 202/204 (99.02%), Postives = 202/204 (99.02%), Query Frame = 1
Query: 322 MITXXXXXXFGMSISRLLVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATEVLP 933
MITSSSLSLFGMSISRLLVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDH KVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATEV P
Sbjct: 1 MITSSSLSLFGMSISRLLVTKSYQNKRVNSGFPGLLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHAKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATEVSP 204
BLAST of Orphan G-protein coupled receptor 22 vs. Planmine SMEST
Match: SMESG000023355.1 (SMESG000023355.1) HSP 1 Score: 95.1301 bits (235), Expect = 1.512e-21 Identity = 92/347 (26.51%), Postives = 163/347 (46.97%), Query Frame = 1
Query: 82 YITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKSYQNKRVNSGFPG--LLILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAY-----CFEKW--DKFEYQFIYY-ISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILF---MSFDNAYKATEVLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIK----LLGQQ*EIHNANYQSL 1071
YI + S++F + GN V++ ++ M++ N +LNLS+AD+ V + +PA L +I W+LG MCK + QGV I++S +L +SI R +R S +LIL+W+ + + +P L F R LET Y C++KW + Q Y+ I M + ++ PL ++ I+YS I F VS+ NL + N ++K ++ ML+I++++FA W+P ++ F + ++ + V+P LG +S ++P +Y+F + +F+ ++ L G++ +YQSL
Sbjct: 28 YIVIYSIVFFFIIAGNTFVILTIVRNAEMKIVINFFILNLSIADICVAIFILPATLIDNITKGWVLGNFMCKSVPFIQGVTISTSVNTLVAISIERYAAICYPLRQRWISKRKCVIILILIWLEALILILPWLLFYTHERTDPI------LETENIYKIWYRCYQKWPLNMLHLQKWYFAIVMFTLLYLCPLVIICISYSLIGFAVSK-----------------------RNLLFQRKCNVIE---KRKIKVIKMLVIVVIMFAFSWLPLYAYNMKQYFGPSTEEQSEFEYSIVVPMIQWLGSSNSCVNPWIYYFFSRNFRVGFMRYLRCLFGKK-----ESYQSL 337
BLAST of Orphan G-protein coupled receptor 22 vs. Planmine SMEST
Match: SMESG000062880.1 (SMESG000062880.1) HSP 1 Score: 93.9745 bits (232), Expect = 4.486e-21 Identity = 85/324 (26.23%), Postives = 158/324 (48.77%), Query Frame = 1
Query: 124 GNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISR--LLVTKSYQNKRVNSGFPGLL---ILVWIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWD-KFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKE--TSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFM---SFDNAYKATE--------VLPYTLLLGYLHSSMSPIVYWFINSSFKQSLIKLLGQQ 1038
GN V++ F+ MR ++ LLNL+ +DL V L+C P + I WI GE +C+++ QG + S L+L + I R ++V K + R N F L+ + +WI S + T P+L + E +L YC E W K + ++ + + ++LPL M+I YS + + R + ++++ K L ++ +S + LN+ ++ + +L+IL L+FAI W+P+ + S +I F+ + DN ++ V P T +L Y++++++P+ I+ F+ +L ++ Q
Sbjct: 87 GNICVILCVTLFRNMRTISHVFLLNLACSDLLVFLICEPFTIASLINPDWIFGESLCRLNGVFQGTSVGVSILTLTSVCIERYIVIVGKGFNQNRFN--FRHLICWVVGIWITSFLITSPLL------QAYEVKEKNLTFIKPTRYCMETWSGKNNRKKVFTTIIFILFYLLPLIFMLILYSKVGNHLRRSTNTLINIENKPSAKRLKTQDTNNVISTHSMSKLNSIKGINNTRQRISKLLMILTLLFAIFWLPYHVTSFIIDFIFGSNIDNNLESFSDVGWLNVYVYPMTQILAYVNNAINPLCTLIISKKFRTTLNQMRKCQ 402
BLAST of Orphan G-protein coupled receptor 22 vs. Planmine SMEST
Match: SMESG000023192.1 (SMESG000023192.1) HSP 1 Score: 90.1225 bits (222), Expect = 6.629e-20 Identity = 94/334 (28.14%), Postives = 162/334 (48.50%), Query Frame = 1
Query: 25 VLDIIYSQYFQPLTRWRL-VYITLCSLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKSYQNKRVNSGFPGLLILV-WIISTVATIPVLYFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKETSLNAHSRE-YRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATEV---LPYTLLLGYLHSSMSPIVYWFINSSFK 1008
++ I S F W++ + I++ S+IF LSV+GNG++V + L K MR S N L+NLS ++ + + I + SI WI G +CK+S + +T + L+ GM+ R V R++S L+I+V W+I+ + + +G + + L+ + C + + E++ Y IS+L + +ILPL V+ ITY I IRL + +G LN RE + K+ +V MLI +I++FA+CW+P+Q ++I NA + + Y L + Y S +P++Y ++ F+
Sbjct: 9 IISISNSTLFDMQVPWKMFLIISVFSIIFFLSVLGNGIMVWIILSQKSMRNSTNYFLVNLSATNVLMAVTHIFPNVYYSINNKWIFGLTLCKLSQFITAFSVTVTVLTFIGMAADRFTVIMYPLRTRLSSKVILLIIVVIWLIAIASGL----------IGA-IKAKIVLDENRKIC-DFLTEDEWKLKYDISLLILQYILPLVVLTITYI--------PISIRLW-----KSTTVG------------ELNRSQRECMKTKRKIVKMLIAIIIIFAVCWLPYQCFFIIIDLNKHLNASPNVLIVFFIIYALAMSY--SFTNPLIYCIMSKKFR 303
BLAST of Orphan G-protein coupled receptor 22 vs. Planmine SMEST
Match: SMESG000066072.1 (SMESG000066072.1) HSP 1 Score: 88.1965 bits (217), Expect = 3.511e-19 Identity = 80/315 (25.40%), Postives = 148/315 (46.98%), Query Frame = 1
Query: 97 SLIFLLSVIGNGLVVILALKFKRMRVSQNICLLNLSLADLGVTLVCIPAELCRSIYTYWILGEVMCKISYYAQGVMITXXXXXXFGMSISRLLVTKSYQNKRVNSGFPG-LLILVWIISTVATIPVL-YFRKTNRLGEYLNISLPLETSIAYCFEKWDKFEYQFIYYISMLAINFILPLFVMIITYSFIVFKVSRLIRIRLIVDDQDHTKVLGYLRNHSNLSKE-TSLNAHSREYRQKKFMVGMLIILILVFAICWMPFQLNSLMILFMSFDNAYKATEVLPYTLLLGYLH------SSMSPIVYWFINSSFKQS 1014
S+IF+L + GN LV+ + L+ K M N + NL+ +D+ + +V +P W L E++CK+ + + S+L+ +SI R V R+ F LL+L+W ++ +A+ P+ Y +K N + +I+ C EKW ++ IY +S + F+LP I SF F++SR ++ R + LSK+ SL+ + ++K+ MLI ++++F +CW+P + ++ + D K L +T + H + +P +Y ++N +F+Q
Sbjct: 35 SMIFVLGLSGNALVIFVILRNKAMYTVTNFFITNLAASDILMCIVAVPFTPIALYLENWTLPELVCKLLPMTMAISVYVSTLTSLVISIDRYFVIVYPFKVRMKLSFCRFLLVLIWGLAILASFPLAKYHKKENDVLR----------NISTCHEKWPDNRWKGIYTVSSFIVQFVLP----TIAISFCYFQISRTLQSRC----------------NRKLSKKIKSLSRVLNDIKRKRKTNKMLIAMVVIFIVCWIPLNVLWIITDLIYSDEQSKLNTNLYFTTIFFICHLIAMCSALYNPFLYGWMNENFRQE 319 The following BLAST results are available for this feature:
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Orphan G-protein coupled receptor 22 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Orphan G-protein coupled receptor 22 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Orphan G-protein coupled receptor 22 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Orphan G-protein coupled receptor 22 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30027972 ID=SMED30027972|Name=Orphan G-protein coupled receptor 22|organism=Schmidtea mediterranea sexual|type=transcript|length=1096bpback to top protein sequence of SMED30027972-orf-1 >SMED30027972-orf-1 ID=SMED30027972-orf-1|Name=SMED30027972-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=347bp MRFNNSAVVLDIIYSQYFQPLTRWRLVYITLCSLIFLLSVIGNGLVVILAback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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