NPHS1-1
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30027960 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Homology
BLAST of NPHS1-1 vs. Ensembl Human
Match: NPHS1 (NPHS1 adhesion molecule, nephrin [Source:HGNC Symbol;Acc:HGNC:7908]) HSP 1 Score: 95.5153 bits (236), Expect = 8.028e-19 Identity = 180/816 (22.06%), Postives = 301/816 (36.89%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQP----PRRRMTKLTVLKV------PSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQL------RLLDERDREMKSSNLHEIGITR------LEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNK---QPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVN 2656
V+EG V + C V+ +QWA G +LG D IP +PR R+ G+ G+ L IE +SD YECQ+ + P PR ++ L K+ T + + GQ N K D I L+G + +VN G++ + T+ TA ++ +SS N ++ C + A E IK V PG VI+ E +V G L+L CV G+P L+W N + A + ++ +++ M+ + + H +L C A + + V +PPS I + + +T E + V LSC S + V + W++ W+ + E+TV S+L D ++ + C S F E ++ I + P+ L + + Q L G +L C GG+P P L R + E +S +H + + E + P DN+ KF C SAS++L V PP ++ I+++ L + C + SS P + W K+ L + P+ ++ S + S+ ++++ + C E + + +S ++ V Y P+FL L VE+G + + A+P+ + W P H S + N Y L +N+ G A L++ Y PT+ A Q+ +VN
Sbjct: 43 VVEGASVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVGRSEMGPELVSPRVILSILVPPKLLLLTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITI-----LLSGQTISDISANVN----------EGSQQKLFTVEATAR-VTPRSSD--NRQLLVCEASSPA-------LEAPIKASFTVNVLFPPGPPVIEWPGLDEG--HVRAGQSLELPCVARG-GNPLATLQWLKNGQPVSTAWG---TEHTQAVARSVLVMT-VRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSASQT-------------ENKNVTLSCVSKS-SRPRVLLRWWL------GWRQL-LPMEETVMDGLHGGHISMSNLTFLARREDNGLT----------LTCEAFSEAFTKETFKKSLILNVKYPAQ-KLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESRLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQ------LSASTQLA---VQFPPT--NVTILANASALRPGDALNLTCVSVSSNPPVNLSW-----------------DKEGERL--EGVAAPPRRAPFKGSAAA--RSVLLQVSSRDHGQRVTCRAHSAE----LRETVSSFYRLNVLYRPEFLGEQVL----VVTAVEQGEAL-LPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSSGALHLWNVTRADDGLYQLHCQNSEGTAEARLRLDVHYAPTIR-ALQDPTEVN 752
BLAST of NPHS1-1 vs. Ensembl Human
Match: NPHS1 (NPHS1 adhesion molecule, nephrin [Source:HGNC Symbol;Acc:HGNC:7908]) HSP 1 Score: 95.1301 bits (235), Expect = 9.673e-19 Identity = 180/816 (22.06%), Postives = 301/816 (36.89%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQP----PRRRMTKLTVLKV------PSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQL------RLLDERDREMKSSNLHEIGITR------LEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNK---QPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVN 2656
V+EG V + C V+ +QWA G +LG D IP +PR R+ G+ G+ L IE +SD YECQ+ + P PR ++ L K+ T + + GQ N K D I L+G + +VN G++ + T+ TA ++ +SS N ++ C + A E IK V PG VI+ E +V G L+L CV G+P L+W N + A + ++ +++ M+ + + H +L C A + + V +PPS I + + +T E + V LSC S + V + W++ W+ + E+TV S+L D ++ + C S F E ++ I + P+ L + + Q L G +L C GG+P P L R + E +S +H + + E + P DN+ KF C SAS++L V PP ++ I+++ L + C + SS P + W K+ L + P+ ++ S + S+ ++++ + C E + + +S ++ V Y P+FL L VE+G + + A+P+ + W P H S + N Y L +N+ G A L++ Y PT+ A Q+ +VN
Sbjct: 43 VVEGASVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVGRSEMGPELVSPRVILSILVPPKLLLLTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITI-----LLSGQTISDISANVN----------EGSQQKLFTVEATAR-VTPRSSD--NRQLLVCEASSPA-------LEAPIKASFTVNVLFPPGPPVIEWPGLDEG--HVRAGQSLELPCVARG-GNPLATLQWLKNGQPVSTAWG---TEHTQAVARSVLVMT-VRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSASQT-------------ENKNVTLSCVSKS-SRPRVLLRWWL------GWRQL-LPMEETVMDGLHGGHISMSNLTFLARREDNGLT----------LTCEAFSEAFTKETFKKSLILNVKYPAQ-KLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESRLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQ------LSASTQLA---VQFPPT--NVTILANASALRPGDALNLTCVSVSSNPPVNLSW-----------------DKEGERL--EGVAAPPRRAPFKGSAAA--RSVLLQVSSRDHGQRVTCRAHSAE----LRETVSSFYRLNVLYRPEFLGEQVL----VVTAVEQGEAL-LPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSSGALHLWNVTRADDGLYQLHCQNSEGTAEARLRLDVHYAPTIR-ALQDPTEVN 752
BLAST of NPHS1-1 vs. Ensembl Human
Match: KIRREL1 (kirre like nephrin family adhesion molecule 1 [Source:HGNC Symbol;Acc:HGNC:15734]) HSP 1 Score: 75.0998 bits (183), Expect = 8.286e-13 Identity = 56/201 (27.86%), Postives = 88/201 (43.78%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVL------KVPSTPII-YQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIND-----TANSLSMKSSQIMNGDIVTCGVENNA 850
V+ G+ ++ C + + G +QW G LG + + +PR R+ G+A G ++L I + ++SD +YECQ + AA R R KLTVL ++ P+I Q G + NLTC + N P T W + G + G S E + D T + L + + + G + TC N A
Sbjct: 32 VVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAAL---RSRRAKLTVLIPPEDTRIDGGPVILLQAGTPH-----------------NLTCRAFNAKPAATIIW------FRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEA 206
BLAST of NPHS1-1 vs. Ensembl Human
Match: KIRREL2 (kirre like nephrin family adhesion molecule 2 [Source:HGNC Symbol;Acc:HGNC:18816]) HSP 1 Score: 68.1662 bits (165), Expect = 5.603e-12 Identity = 51/159 (32.08%), Postives = 70/159 (44.03%), Query Frame = 2
Query: 251 CFR--ELPSPKY-------DVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSL-NGNPVPTFEW 697
CFR PSP + VL G++ + C + G +QW G LG R++P + R ++GNA G H L I V++ D +YECQ + A R R +L VL P P + SVS+ NLTC S + P P W
Sbjct: 15 CFRGRAGPSPHFLQQPEDLVVLLGEEARLPCALGAYWGLVQWTKSGLALGGQRDLPGWSRYWISGNAANGQHDLHIRPVELEDEASYECQATQAGL---RSRPAQLHVLVPPEAPQVL-------GGPSVSLVA---GVPANLTCRSRGDARPTPELLW 160
BLAST of NPHS1-1 vs. Ensembl Human
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:HGNC Symbol;Acc:HGNC:23204]) HSP 1 Score: 72.4034 bits (176), Expect = 5.906e-12 Identity = 64/251 (25.50%), Postives = 102/251 (40.64%), Query Frame = 2
Query: 158 PFNVDDLDA-----AESISLSQSSTC-----TEKDDIHR-----IQCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIND-----TANSLSMKSSQIMNGDIVTCGVENNA 850
PF +D L ++ + L + C KD R + F + P + V+ G+ V + C + G + W G LG R++ +YP+ + GN + G+H L I ++ D YECQ AA R R +LTVL P P+I +S++ + +NLTC + N P + W L+ G G S + D ++L + + NG + C N A
Sbjct: 3 PFQLDLLFVCFFLFSQELGLQKRGCCLVLGYMAKDKFRRMNEGQVYSFSQQPQDQV-VVSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVGNHLSGEHHLKILRAELQDDAVYECQAIQAAI---RSRPARLTVLVPPDDPVILGGP-------VISLRAGD---PLNLTCHADNAKPAASIIW------LRKGEVINGATYSKTLLRDGKRESIVSTLFISPGDVENGQSIVCRATNKA 233
BLAST of NPHS1-1 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 135.961 bits (341), Expect = 1.198e-31 Identity = 262/1225 (21.39%), Postives = 470/1225 (38.37%), Query Frame = 2
Query: 248 QCFRELPSPKYDVLEGKDVLMGCRVAHQQGKL---QW-AFKGKMLGFDRN--IPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQ-LSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDT---ANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDN-LIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMAN----YSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPT-RPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESL--DNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYI-CVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDT----VVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSREK 3853
Q F E PS + G+ + C + QW + G +LG+ + +P + + L I +V + D G YECQ L P + P R + L ++ VP + + N Q N SI + ++ +N+TCV N P P W ++ + K + S +N T SL ++S + +G ++TC + F I T + P E+ + + G + ++C G+PP D+ W ++ + L +S+ +N + S+ + E C + P + + VNYPP+ +E+ ++N+ + + + CK S +I W I + + T ++ +G I SS V + + +H ++ V C+ + E + +Q I++ P P++T L + G+ + C GG+P +L ++ + N + + V N ++ +C + N +D S+ L V PP+++ ++ S D L+ ++ + C T SS P A I W + R+T + L + ++ + +FT T E+D + + C I H +W ++ + V YPP+ L ND T V+ +G++F + +P+I+ + W N P + H V V + ++++ Y+L N++G L + Y + S I ++ C A PS+ + + W + + E L +G +K I +R T Q + G Y C N P +T LV R PV + K+ A + G ++ C + VPTV+++ + K+E L N KY Q + +++ L I + D K + C+A N +G + + P P +P N T+ N T ++SV W RF+GGS Q ++ +R ++ +VN + N + + FF I S+N ++ P+I T+N + G N + R E ++ ++ IL+ V +LL + +I FV R K RK REK
Sbjct: 19 QFFLESPS-NLSTIAGESITFRCSAEKSPEPIVYSQWKSNTGSLLGYHQEGILPGHQGRFSYIKQNAEELHLKITHVNLDDDGEYECQMLHP--EEGPIRAKSFLNII-VPPQLVYFSNYQPN------SIIAVKENTPLNITCVVPNVKPEPEVLWYMDGKVMSRDVK----QASTPHLNKTFTVYTSLVVQSDRNDHGKVITC--------EAFQKETDIRITTNTTLDVLFPPSDPTVEI--LRNPSALRSGDNVTIACSVTG-GNPPPDVFWY--HENKRLQSHSTLDTRSKEI-KNIYSFI-ASQNDNMAEYECRANNSRTGNPKRKAMKLEVNYPPASVELFG---ESNIRYGSSANIQCKSLPSNPAS-----------QITWIING------RSVPTPTQREFVVENG----IVSSSNVSVHSNELSVEAHQIN-----VECMATNPEGSSA--KQHVIKIIAPPKAPIIT--GLEDRKFF-EGDIVNVTCEAQGGNPLAELSWYRGSEKVNGAHNEVAGFSSYSTLALRVDRTMNTQRLKCEAT-NAALDEPLIESQY--LSVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFS-----------RATDDNKVHLGDVSLNETTRDNGFNVENVLSFTP-----TEEYDGTVVHC-IANHPEW---KHSVNTSFPLNVMYPPKML-----VNDPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFD---HTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAAT-----GDTVLLECEADGEPSKANM-IHWYK------NGEILAAQHRGHKKAI----LRLNATEQQS------------------------------GEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKT--AGILGGVAKARCKV--YAVPTVEFV-WEKDEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQNDDLIHLVNTTHTNLRLSGLATANTYFFQIR-------------SINARGFASSWTSPVIFATLN---------------EDGVNVAM-IRNEKLKLTTWIPYILIFIVLFMLLNV---VICCFVCNRT-KKRKLREK 1065
BLAST of NPHS1-1 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 132.88 bits (333), Expect = 8.752e-31 Identity = 266/1238 (21.49%), Postives = 477/1238 (38.53%), Query Frame = 2
Query: 248 QCFRELPSPKYDVLEGKDVLMGCRVAHQQGKL---QW-AFKGKMLGFDRN--IPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQ-LSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDT---ANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQL-------RLLDERDREMKSSNLHEI-----GITRLE-YNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDN-LIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMAN----YSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPT-RPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESL--DNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYI-CVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDT----VVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSREK 3853
Q F E PS + G+ + C + QW + G +LG+ + +P + + L I +V + D G YECQ L P + P R + L ++ VP + + N Q N SI + ++ +N+TCV N P P W ++ + K + S +N T SL ++S + +G ++TC + F I T + P E+ + + G + ++C G+PP D+ W ++ + L +S+ +N + S+ + E C + P + + VNYPP+ +E+ ++N+ + + + CK S +I W I + + T ++ +G I SS V + + +H ++ V C+ + E + +Q I++ P P++T L + G+ + C GG+P +L + L + ++MK S ++ G + + V N ++ +C + N +D S+ L V PP+++ ++ S D L+ ++ + C T SS P A I W + R+T + L + ++ + +FT T E+D + + C I H +W ++ + V YPP+ L ND T V+ +G++F + +P+I+ + W N P + H V V + ++++ Y+L N++G L + Y + S I ++ C A PS+ + + W + + E L +G +K I +R T Q + G Y C N P +T LV R PV + K+ A + G ++ C + VPTV+++ + K+E L N KY Q + +++ L I + D K + C+A N +G + + P P +P N T+ N T ++SV W RF+GGS Q ++ +R ++ +VN + N + + FF I S+N ++ P+I T+N + G N + R E ++ ++ IL+ V +LL + +I FV R K RK REK
Sbjct: 19 QFFLESPS-NLSTIAGESITFRCSAEKSPEPIVYSQWKSNTGSLLGYHQEGILPGHQGRFSYIKQNAEELHLKITHVNLDDDGEYECQMLHP--EEGPIRAKSFLNII-VPPQLVYFSNYQPN------SIIAVKENTPLNITCVVPNVKPEPEVLWYMDGKVMSRDVK----QASTPHLNKTFTVYTSLVVQSDRNDHGKVITC--------EAFQKETDIRITTNTTLDVLFPPSDPTVEI--LRNPSALRSGDNVTIACSVTG-GNPPPDVFWY--HENKRLQSHSTLDTRSKEI-KNIYSFI-ASQNDNMAEYECRANNSRTGNPKRKAMKLEVNYPPASVELFG---ESNIRYGSSANIQCKSLPSNPAS-----------QITWIING------RSVPTPTQREFVVENG----IVSSSNVSVHSNELSVEAHQIN-----VECMATNPEGSSA--KQHVIKIIAPPKAPIIT--GLEDRKFF-EGDIVNVTCEAQGGNPLAELSWYRGSEKHLVKGRKKMKWSQVNGAHNEVAGFSSYSTLALRVDRTMNTQRLKCEAT-NAALDEPLIESQY--LSVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFS-----------RATDDNKVHLGDVSLNETTRDNGFNVENVLSFTP-----TEEYDGTVVHC-IANHPEW---KHSVNTSFPLNVMYPPKML-----VNDPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFD---HTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAAT-----GDTVLLECEADGEPSKANM-IHWYK------NGEILAAQHRGHKKAI----LRLNATEQQS------------------------------GEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKT--AGILGGVAKARCKV--YAVPTVEFV-WEKDEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQNDDLIHLVNTTHTNLRLSGLATANTYFFQIR-------------SINARGFASSWTSPVIFATLN---------------EDGVNVAM-IRNEKLKLTTWIPYILIFIVLFMLLNV---VICCFVCNRT-KKRKLREK 1078
BLAST of NPHS1-1 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 109.768 bits (273), Expect = 9.643e-24 Identity = 194/910 (21.32%), Postives = 348/910 (38.24%), Query Frame = 2
Query: 1163 ELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDN-LIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMAN----YSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPT-RPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESL--DNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYI-CVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDT----VVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSREK 3853
E C + P + + VNYPP+ +E+ ++N+ + + + CK S +I W I + + T ++ +G I SS V + + +H ++ V C+ + E + +Q I++ P P++T L + G+ + C GG+P +L ++ + N + + V N ++ +C + N +D S+ L V PP+++ ++ S D L+ ++ + C T SS P A I W + R+T + L + ++ + +FT T E+D + + C I H +W ++ + V YPP+ L ND T V+ +G++F + +P+I+ + W N P + H V V + ++++ Y+L N++G L + Y + S I ++ C A PS+ + + W + + E L +G +K I +R T Q + G Y C N P +T LV R PV + K+ A + G ++ C + VPTV+++ + K+E L N KY Q + +++ L I + D K + C+A N +G + + P P +P N T+ N T ++SV W RF+GGS Q ++ +R ++ +VN + N + + FF I S+N ++ P+I T+N + G N + R E ++ ++ IL+ V +LL + +I FV R K RK REK
Sbjct: 3 EYECRANNSRTGNPKRKAMKLEVNYPPASVELFG---ESNIRYGSSANIQCKSLPSNPAS-----------QITWIING------RSVPTPTQREFVVENG----IVSSSNVSVHSNELSVEAHQIN-----VECMATNPEGSSA--KQHVIKIIAPPKAPIIT--GLEDRKFF-EGDIVNVTCEAQGGNPLAELSWYRGSEKVNGAHNEVAGFSSYSTLALRVDRTMNTQRLKCEAT-NAALDEPLIESQY--LSVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFS-----------RATDDNKVHLGDVSLNETTRDNGFNVENVLSFTP-----TEEYDGTVVHC-IANHPEW---KHSVNTSFPLNVMYPPKML-----VNDPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFD---HTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAAT-----GDTVLLECEADGEPSKANM-IHWYK------NGEILAAQHRGHKKAI----LRLNATEQQS------------------------------GEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKT--AGILGGVAKARCKV--YAVPTVEFV-WEKDEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQNDDLIHLVNTTHTNLRLSGLATANTYFFQIR-------------SINARGFASSWTSPVIFATLN---------------EDGVNVAM-IRNEKLKLTTWIPYILIFIVLFMLLNV---VICCFVCNRT-KKRKLREK 763
BLAST of NPHS1-1 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 107.071 bits (266), Expect = 5.715e-23 Identity = 198/923 (21.45%), Postives = 355/923 (38.46%), Query Frame = 2
Query: 1163 ELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQL-------RLLDERDREMKSSNLHEI-----GITRLE-YNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDN-LIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMAN----YSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPT-RPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESL--DNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYI-CVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDT----VVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSREK 3853
E C + P + + VNYPP+ +E+ ++N+ + + + CK S +I W I + + T ++ +G I SS V + + +H ++ V C+ + E + +Q I++ P P++T L + G+ + C GG+P +L + L + ++MK S ++ G + + V N ++ +C + N +D S+ L V PP+++ ++ S D L+ ++ + C T SS P A I W + R+T + L + ++ + +FT T E+D + + C I H +W ++ + V YPP+ L ND T V+ +G++F + +P+I+ + W N P + H V V + ++++ Y+L N++G L + Y + S I ++ C A PS+ + + W + + E L +G +K I +R T Q + G Y C N P +T LV R PV + K+ A + G ++ C + VPTV+++ + K+E L N KY Q + +++ L I + D K + C+A N +G + + P P +P N T+ N T ++SV W RF+GGS Q ++ +R ++ +VN + N + + FF I S+N ++ P+I T+N + G N + R E ++ ++ IL+ V +LL + +I FV R K RK REK
Sbjct: 3 EYECRANNSRTGNPKRKAMKLEVNYPPASVELFG---ESNIRYGSSANIQCKSLPSNPAS-----------QITWIING------RSVPTPTQREFVVENG----IVSSSNVSVHSNELSVEAHQIN-----VECMATNPEGSSA--KQHVIKIIAPPKAPIIT--GLEDRKFF-EGDIVNVTCEAQGGNPLAELSWYRGSEKHLVKGRKKMKWSQVNGAHNEVAGFSSYSTLALRVDRTMNTQRLKCEAT-NAALDEPLIESQY--LSVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFS-----------RATDDNKVHLGDVSLNETTRDNGFNVENVLSFTP-----TEEYDGTVVHC-IANHPEW---KHSVNTSFPLNVMYPPKML-----VNDPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFD---HTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAAT-----GDTVLLECEADGEPSKANM-IHWYK------NGEILAAQHRGHKKAI----LRLNATEQQS------------------------------GEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKT--AGILGGVAKARCKV--YAVPTVEFV-WEKDEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQNDDLIHLVNTTHTNLRLSGLATANTYFFQIR-------------SINARGFASSWTSPVIFATLN---------------EDGVNVAM-IRNEKLKLTTWIPYILIFIVLFMLLNV---VICCFVCNRT-KKRKLREK 776
BLAST of NPHS1-1 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 100.523 bits (249), Expect = 5.297e-21 Identity = 154/682 (22.58%), Postives = 268/682 (39.30%), Query Frame = 2
Query: 1847 NEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDN-LIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMAN----YSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPT-RPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESL--DNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYI-CVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDT----VVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSREK 3853
N ++ +C + N +D S+ L V PP+++ ++ S D L+ ++ + C T SS P A I W + R+T + L + ++ + +FT T E+D + + C I H +W ++ + V YPP+ L ND T V+ +G++F + +P+I+ + W N P + H V V + ++++ Y+L N++G L + Y + S I ++ C A PS+ + + W + +IL + +G +K I +R T Q + G Y C N P +T LV R PV + K+ A + G ++ C + VPTV+++ + K+E L N KY Q + +++ L I + D K + C+A N +G + + P P +P N T+ N T ++SV W RF+GGS Q ++ +R ++ +VN + N + + FF I S+N ++ P+I T+N + G N + R E ++ ++ IL+ V +LL + +I FV R K RK REK
Sbjct: 2 NTQRLKCEAT-NAALDEPLIESQY--LSVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFS-----------RATDDNKVHLGDVSLNETTRDNGFNVENVLSFTP-----TEEYDGTVVHC-IANHPEW---KHSVNTSFPLNVMYPPKML-----VNDPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFD---HTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAAT-----GDTVLLECEADGEPSKANM-IHWYKN-GEILAAQH-----RGHKKAI----LRLNATEQQS------------------------------GEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKT--AGILGGVAKARCKV--YAVPTVEFV-WEKDEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQNDDLIHLVNTTHTNLRLSGLATANTYFFQIR-------------SINARGFASSWTSPVIFATLN---------------EDGVNVAM-IRNEKLKLTTWIPYILIFIVLFMLLNV---VICCFVCNRT-KKRKLREK 568
BLAST of NPHS1-1 vs. Ensembl Fly
Match: hbs (gene:FBgn0029082 transcript:FBtr0087414) HSP 1 Score: 248.44 bits (633), Expect = 1.997e-66 Identity = 280/1208 (23.18%), Postives = 491/1208 (40.65%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTA-------NSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQV-VCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNL-----------GKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSRE 3850
+LEG D L+ C V+++ GK+QW G LGF IP +PR + +A ++L I+N + D Y+CQ+ PAA P R KL+++ PS+ +I + +N V +++ + N+ L C++ N NP W P+ + I T+N TA ++L ++ + +C + A + ++ Q+++ PG + S E + G ++Q++C G+PP L W N + Q S S+N ++ + + + L C P A + + V Y P + + + K G V+LSC + RI W I +P+ S KT +SDG +++SS +IS I S+ + + VC +TE + + + PS P+LT N IL +G KL+C+ GG+P P L+ S L + IT E ++ V DN ++C V N +D +++ + + PP+ + + ++ NL+ ++ C + SS P AKI WW KD + + N P ST + V +T + D C H + + + + + I + + YPP+F + P TFV +G F + + + +P + YTW + P+ S+ +++ P + N S L N+ G A + + + Y PT+ S+ V A++ C QA P E V W R D +L + ISS + G +QI ++ IG++ CI+ N + PA + + LV T P + + +AA++ +++L C + P P+ W K+ + K+ + Q + F++ L I +T+ D G+Y CV N G++S + +KP P P+ V NV++T V + W F+GG ++ Y+RLR + ++ + D + Q ++ L Y S+++ NE S +P I+ +G + E E N ++II + + ++LL + L+ +FV +R+ K + E
Sbjct: 50 ILEGTDTLLRCEVSNRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGPAAGNPAIRANAKLSIVAAPSSIVI----EGYARNARVEVEERQ---NLTLHCIAENANPAAEIVWFQGEVPV--------STAPIVTVNQTAPKRFTTSSTLHLQPRAEDDYKEFSCEARHKALPP-----DVPMRAQVQLSVLYPPGAPFFEGYSQGET---LHRGQEVQIACRSRG-GNPPAQLTWYRNGVAISSPQRT-----SGRLSENVYKFTAAAED-NGANLVCEAKNLLATTPLRAELNLTVLYAPKDVYLSGAN-------------QAKVGDSVQLSCVTAPS-NPQARISWSING------RPLDNSTYKTTSSSDG--GWVSSS----------NISLTIDSQSRTFIAVCHALNTELTQNVVGSHTVNVLYPPSPPLLTGYN--DGDILISGSILKLQCSSAGGNPPPTLQWYKNDKIINAPSKLVDSKITS-ELSLLVNASDNNAIYKCKVQ-NAAIDIPLFATKTLGVHF--PPETVKISVVPK--NLVPGIRAKLICDSSSSNPPAKISWW-----------------KDGIPVEGLNLANRPGLWGGSVSTLEMY----VNITQDLDGIVYTCQ--SHNE--VLQRSVHETISLDILYPPKF----ETPQST-TFVGVEGAPFHVELLASGNPMVITYTWTKDGLPISSN-----SLSGQRLISDGPRL--NISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAPTITAVSEGRSFV-----AGEPAVLACHIQARP-LEAAHVRWSR------DGYDL------ATRTISS----------FENG--------------TALLQIASVERSDIGNFTCIVDN-QRGAPAAQNVLLVVQTAPEIDHSPGFTRYAARLGVRAQLICRSLASPQPSFIWRRHGKDLKMQRRNKFKSVERQVDALNFESALLIENTSPDDYGQYECVVRNSLGQASTTLEFSKPSRPDAPLQLRVGNVSDTGVDLNWTPGFDGG----MQTYFRLR---LKQHGEDKYKYVDAKPGHQNISLDGLKPGATYYFSVMAANEAGGSKFMPD------------------IKLTLSKGSQPHSAEYTEKDELPN-VMIIGITSAAMVLLVLNAALVAWFVIRRQNKSQSEAE 1081
BLAST of NPHS1-1 vs. Ensembl Fly
Match: hbs (gene:FBgn0029082 transcript:FBtr0345419) HSP 1 Score: 248.44 bits (633), Expect = 1.997e-66 Identity = 280/1208 (23.18%), Postives = 491/1208 (40.65%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTA-------NSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQV-VCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNL-----------GKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSRE 3850
+LEG D L+ C V+++ GK+QW G LGF IP +PR + +A ++L I+N + D Y+CQ+ PAA P R KL+++ PS+ +I + +N V +++ + N+ L C++ N NP W P+ + I T+N TA ++L ++ + +C + A + ++ Q+++ PG + S E + G ++Q++C G+PP L W N + Q S S+N ++ + + + L C P A + + V Y P + + + K G V+LSC + RI W I +P+ S KT +SDG +++SS +IS I S+ + + VC +TE + + + PS P+LT N IL +G KL+C+ GG+P P L+ S L + IT E ++ V DN ++C V N +D +++ + + PP+ + + ++ NL+ ++ C + SS P AKI WW KD + + N P ST + V +T + D C H + + + + + I + + YPP+F + P TFV +G F + + + +P + YTW + P+ S+ +++ P + N S L N+ G A + + + Y PT+ S+ V A++ C QA P E V W R D +L + ISS + G +QI ++ IG++ CI+ N + PA + + LV T P + + +AA++ +++L C + P P+ W K+ + K+ + Q + F++ L I +T+ D G+Y CV N G++S + +KP P P+ V NV++T V + W F+GG ++ Y+RLR + ++ + D + Q ++ L Y S+++ NE S +P I+ +G + E E N ++II + + ++LL + L+ +FV +R+ K + E
Sbjct: 50 ILEGTDTLLRCEVSNRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGPAAGNPAIRANAKLSIVAAPSSIVI----EGYARNARVEVEERQ---NLTLHCIAENANPAAEIVWFQGEVPV--------STAPIVTVNQTAPKRFTTSSTLHLQPRAEDDYKEFSCEARHKALPP-----DVPMRAQVQLSVLYPPGAPFFEGYSQGET---LHRGQEVQIACRSRG-GNPPAQLTWYRNGVAISSPQRT-----SGRLSENVYKFTAAAED-NGANLVCEAKNLLATTPLRAELNLTVLYAPKDVYLSGAN-------------QAKVGDSVQLSCVTAPS-NPQARISWSING------RPLDNSTYKTTSSSDG--GWVSSS----------NISLTIDSQSRTFIAVCHALNTELTQNVVGSHTVNVLYPPSPPLLTGYN--DGDILISGSILKLQCSSAGGNPPPTLQWYKNDKIINAPSKLVDSKITS-ELSLLVNASDNNAIYKCKVQ-NAAIDIPLFATKTLGVHF--PPETVKISVVPK--NLVPGIRAKLICDSSSSNPPAKISWW-----------------KDGIPVEGLNLANRPGLWGGSVSTLEMY----VNITQDLDGIVYTCQ--SHNE--VLQRSVHETISLDILYPPKF----ETPQST-TFVGVEGAPFHVELLASGNPMVITYTWTKDGLPISSN-----SLSGQRLISDGPRL--NISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAPTITAVSEGRSFV-----AGEPAVLACHIQARP-LEAAHVRWSR------DGYDL------ATRTISS----------FENG--------------TALLQIASVERSDIGNFTCIVDN-QRGAPAAQNVLLVVQTAPEIDHSPGFTRYAARLGVRAQLICRSLASPQPSFIWRRHGKDLKMQRRNKFKSVERQVDALNFESALLIENTSPDDYGQYECVVRNSLGQASTTLEFSKPSRPDAPLQLRVGNVSDTGVDLNWTPGFDGG----MQTYFRLR---LKQHGEDKYKYVDAKPGHQNISLDGLKPGATYYFSVMAANEAGGSKFMPD------------------IKLTLSKGSQPHSAEYTEKDELPN-VMIIGITSAAMVLLVLNAALVAWFVIRRQNKSQSEAE 1081
BLAST of NPHS1-1 vs. Ensembl Fly
Match: hbs (gene:FBgn0029082 transcript:FBtr0087413) HSP 1 Score: 248.44 bits (633), Expect = 2.122e-66 Identity = 280/1208 (23.18%), Postives = 491/1208 (40.65%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTA-------NSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQV-VCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNL-----------GKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSRE 3850
+LEG D L+ C V+++ GK+QW G LGF IP +PR + +A ++L I+N + D Y+CQ+ PAA P R KL+++ PS+ +I + +N V +++ + N+ L C++ N NP W P+ + I T+N TA ++L ++ + +C + A + ++ Q+++ PG + S E + G ++Q++C G+PP L W N + Q S S+N ++ + + + L C P A + + V Y P + + + K G V+LSC + RI W I +P+ S KT +SDG +++SS +IS I S+ + + VC +TE + + + PS P+LT N IL +G KL+C+ GG+P P L+ S L + IT E ++ V DN ++C V N +D +++ + + PP+ + + ++ NL+ ++ C + SS P AKI WW KD + + N P ST + V +T + D C H + + + + + I + + YPP+F + P TFV +G F + + + +P + YTW + P+ S+ +++ P + N S L N+ G A + + + Y PT+ S+ V A++ C QA P E V W R D +L + ISS + G +QI ++ IG++ CI+ N + PA + + LV T P + + +AA++ +++L C + P P+ W K+ + K+ + Q + F++ L I +T+ D G+Y CV N G++S + +KP P P+ V NV++T V + W F+GG ++ Y+RLR + ++ + D + Q ++ L Y S+++ NE S +P I+ +G + E E N ++II + + ++LL + L+ +FV +R+ K + E
Sbjct: 50 ILEGTDTLLRCEVSNRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGPAAGNPAIRANAKLSIVAAPSSIVI----EGYARNARVEVEERQ---NLTLHCIAENANPAAEIVWFQGEVPV--------STAPIVTVNQTAPKRFTTSSTLHLQPRAEDDYKEFSCEARHKALPP-----DVPMRAQVQLSVLYPPGAPFFEGYSQGET---LHRGQEVQIACRSRG-GNPPAQLTWYRNGVAISSPQRT-----SGRLSENVYKFTAAAED-NGANLVCEAKNLLATTPLRAELNLTVLYAPKDVYLSGAN-------------QAKVGDSVQLSCVTAPS-NPQARISWSING------RPLDNSTYKTTSSSDG--GWVSSS----------NISLTIDSQSRTFIAVCHALNTELTQNVVGSHTVNVLYPPSPPLLTGYN--DGDILISGSILKLQCSSAGGNPPPTLQWYKNDKIINAPSKLVDSKITS-ELSLLVNASDNNAIYKCKVQ-NAAIDIPLFATKTLGVHF--PPETVKISVVPK--NLVPGIRAKLICDSSSSNPPAKISWW-----------------KDGIPVEGLNLANRPGLWGGSVSTLEMY----VNITQDLDGIVYTCQ--SHNE--VLQRSVHETISLDILYPPKF----ETPQST-TFVGVEGAPFHVELLASGNPMVITYTWTKDGLPISSN-----SLSGQRLISDGPRL--NISRLSRNDAGVYICEALNSQGTALLEIQVAVEYAPTITAVSEGRSFV-----AGEPAVLACHIQARP-LEAAHVRWSR------DGYDL------ATRTISS----------FENG--------------TALLQIASVERSDIGNFTCIVDN-QRGAPAAQNVLLVVQTAPEIDHSPGFTRYAARLGVRAQLICRSLASPQPSFIWRRHGKDLKMQRRNKFKSVERQVDALNFESALLIENTSPDDYGQYECVVRNSLGQASTTLEFSKPSRPDAPLQLRVGNVSDTGVDLNWTPGFDGG----MQTYFRLR---LKQHGEDKYKYVDAKPGHQNISLDGLKPGATYYFSVMAANEAGGSKFMPD------------------IKLTLSKGSQPHSAEYTEKDELPN-VMIIGITSAAMVLLVLNAALVAWFVIRRQNKSQSEAE 1081
BLAST of NPHS1-1 vs. Ensembl Fly
Match: sns (gene:FBgn0024189 transcript:FBtr0088635) HSP 1 Score: 194.126 bits (492), Expect = 2.953e-49 Identity = 278/1215 (22.88%), Postives = 473/1215 (38.93%), Query Frame = 2
Query: 248 QCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIN--DTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYA-IPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTL-GIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSK----LIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNK----QPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVE-------SNNFLII-ILLATVGVLLLTIKIGLIVYFVRKRKLK 3832
Q FR P VLEG + +M C VA+ G +QW G LGF IP +PR + G+ G ++L I N ++D +Y+CQ+ PA R KLTV+ P++ I G S+ + V +D + L C+ N P W R +++ + R + V T T +SL +K + TC + A + + Q+ V PG I+ S E + G ++L C G+PP L W N + +A S S+N + + + + DN A A+ ++ N +++E+ + T+V++ P+ + G V L+CT+ I W + + + + KT+ + +G TS++ + V+ + S + V+C + + + I + P+ P+++ + QI+ G KL C +GG+P L + D+ + S E I DN+ ++RC S N+ + S +++ A P+ + ++I + L + C + SS P AK+ WW K + GI +TSK T ST V +T E + C + N+ L + + + V Y P+F +P T V +G + +++++ A+P+ Y W + Q + +I D F+ ++ + YS N+ G A + + + YG T+ S E I VNP AM+ C+ + P E+ V+W R + D T+T N S+ + I +G++RC+ N P + I L+ P K A+ + L C G P P W K+ ++ KY + + +++ L + +D G Y CVA N+ GE+ V L P P++ ++NVT +V++ W F+GG K ++ +R+ D Y+ + + K TI L N Y S++S NE S+ +P D R K LE + P E + N +II I LA G LL+ + + +FV +R+ K
Sbjct: 72 QKFRTTPH-DLQVLEGAEAMMRCEVANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGPARLNSAIRANAKLTVISPPAS--IEIKGYSHNSKVEVRENQD-----LQLKCIVANAKPAAQIVWY--RGNVEYKPEKREDTVEESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKALSPDMPMRATV---QLSVL--YPPGPPYIEGYSAGET---LRRGQTVELMCRSRG-GNPPAQLIWYKNGSQIRMAYR-----TSGRLSENIY-------------TFTAEAGDNKARFRCEASNVMSQNPLKAEVELSVLFAPTHVTVMGPTE--ARVGDIVPLTCTTAP-SNPPAEIKWMVGGRQ------VRNATSKTIVSPEGGWT-TTSNIT---AVVEPNKRSLV-------VICHGLNMQLTENVVSTHTINVLYPPAPPLIS--GYMEGQIIPAGSVQKLLCVSSGGNPLATLTWY-KNDKRINSVIRAADKSVSAEITILANVSDNQAQYRCEAS-NSATEIPLFQSTTLSVHFA--PETVKIRI--EPEELRPGMEATIICDSSSSNPPAKLSWW-----KDGIPIEGINNTSKP----------------GLWGGTVSTL-EFRVNVTQEMNGQVYTCQ--------SANEALQRSAHEAVSLDVLYRPKF-----VPPPSSTAVGVEGESLQVSLQTRANPTPVTYKWTKDGTTIPQDGDHRIFADGGSLNFTRLHRDDAGI--YSCSASNSQGGATLNITVVVEYGTTIKSVS-ENIVVNP----GEDAMLSCTVEGKPLTEE-HVKWER---------------------VGYDMTVKTST-------TFANGTSY--------LHIKDAKREDVGNFRCVADNRVDN-PTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSPKPQFIWRQDKKDLPINRTYKYEVEERKIDSLTYESTLIVDKVAPADYGAYECVARNELGEAVETVRLEITSQPDPPLSLNILNVTHDTVTLAWTPGFDGGLKASYRVRYRMAD---REQYKYIDGLPNSHK----LTIGGLRMNTLYLFSVMSWNELGQSSYLP----------------------DLARAETKGLE--LMPAELTEDSSNTPNLVIIGISLAAFGFLLVNASL-VAWFFVHQRRKK 1109
BLAST of NPHS1-1 vs. Ensembl Fly
Match: sns (gene:FBgn0024189 transcript:FBtr0339277) HSP 1 Score: 189.889 bits (481), Expect = 4.709e-48 Identity = 258/1132 (22.79%), Postives = 444/1132 (39.22%), Query Frame = 2
Query: 248 QCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIN--DTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYA-IPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTL-GIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSK----LIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNK----QPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIP 3607
Q FR P VLEG + +M C VA+ G +QW G LGF IP +PR + G+ G ++L I N ++D +Y+CQ+ PA R KLTV+ P++ I G S+ + V +D + L C+ N P W R +++ + R + V T T +SL +K + TC + A + + Q+ V PG I+ S E + G ++L C G+PP L W N + +A S S+N + + + + DN A A+ ++ N +++E+ + T+V++ P+ + G V L+CT+ I W + + + + KT+ + +G TS++ + V+ + S + V+C + + + I + P+ P+++ + QI+ G KL C +GG+P L + D+ + S E I DN+ ++RC S N+ + S +++ A P+ + ++I + L + C + SS P AK+ WW K + GI +TSK T ST V +T E + C + N+ L + + + V Y P+F +P T V +G + +++++ A+P+ Y W + Q + +I D F+ ++ + YS N+ G A + + + YG T+ S E I VNP AM+ C+ + P E+ V+W R + D T+T N S+ + I +G++RC+ N P + I L+ P K A+ + L C G P P W K+ ++ KY + + +++ L + +D G Y CVA N+ GE+ V L P P++ ++NVT +V++ W F+GG K ++ +R+ D Y+ + + K TI L N Y S++S NE S+ +P
Sbjct: 72 QKFRTTPH-DLQVLEGAEAMMRCEVANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGPARLNSAIRANAKLTVISPPAS--IEIKGYSHNSKVEVRENQD-----LQLKCIVANAKPAAQIVWY--RGNVEYKPEKREDTVEESTAKRFTTTSSLKLKPGPDDDYTEYTCQARHKALSPDMPMRATV---QLSVL--YPPGPPYIEGYSAGET---LRRGQTVELMCRSRG-GNPPAQLIWYKNGSQIRMAYR-----TSGRLSENIY-------------TFTAEAGDNKARFRCEASNVMSQNPLKAEVELSVLFAPTHVTVMGPTE--ARVGDIVPLTCTTAP-SNPPAEIKWMVGGRQ------VRNATSKTIVSPEGGWT-TTSNIT---AVVEPNKRSLV-------VICHGLNMQLTENVVSTHTINVLYPPAPPLIS--GYMEGQIIPAGSVQKLLCVSSGGNPLATLTWY-KNDKRINSVIRAADKSVSAEITILANVSDNQAQYRCEAS-NSATEIPLFQSTTLSVHFA--PETVKIRI--EPEELRPGMEATIICDSSSSNPPAKLSWW-----KDGIPIEGINNTSKP----------------GLWGGTVSTL-EFRVNVTQEMNGQVYTCQ--------SANEALQRSAHEAVSLDVLYRPKF-----VPPPSSTAVGVEGESLQVSLQTRANPTPVTYKWTKDGTTIPQDGDHRIFADGGSLNFTRLHRDDAGI--YSCSASNSQGGATLNITVVVEYGTTIKSVS-ENIVVNP----GEDAMLSCTVEGKPLTEE-HVKWER---------------------VGYDMTVKTST-------TFANGTSY--------LHIKDAKREDVGNFRCVADNRVDN-PTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSPKPQFIWRQDKKDLPINRTYKYEVEERKIDSLTYESTLIVDKVAPADYGAYECVARNELGEAVETVRLEITSQPDPPLSLNILNVTHDTVTLAWTPGFDGGLKASYRVRYRMAD---REQYKYIDGLPNSHK----LTIGGLRMNTLYLFSVMSWNELGQSSYLP 1051
BLAST of NPHS1-1 vs. Ensembl Zebrafish
Match: kirrel3a (kirre like nephrin family adhesion molecule 3a [Source:ZFIN;Acc:ZDB-GENE-091117-44]) HSP 1 Score: 82.4185 bits (202), Expect = 3.859e-15 Identity = 53/195 (27.18%), Postives = 88/195 (45.13%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIND-----TANSLSMKSSQIMNGDIVTCGVENNAT 853
++ G+ V + C + QG + W G LG +R++ YPR + G+ G++ L IE ++ D G++ECQ A R R +LTVL P P+I I + ++NLTC + N P + W +++G G + + D T ++L + +S I +G +TC N A
Sbjct: 32 IVAGEPVTLPCTIIGYQGMVLWVKDGLALGVNRDLSGYPRYDVIGDHSKGEYHLLIERTELVDDGSFECQAIQVAG---RSRPAQLTVLVPPDDPVI----------IGAPVVSLRAGDSLNLTCHANNAKPAASIIW------IRNGEVINGAAYTKTLLRDGKRESTVSTLHISASNIESGQQITCRASNKAA 207
BLAST of NPHS1-1 vs. Ensembl Zebrafish
Match: kirrel3a (kirre like nephrin family adhesion molecule 3a [Source:ZFIN;Acc:ZDB-GENE-091117-44]) HSP 1 Score: 82.4185 bits (202), Expect = 3.859e-15 Identity = 53/195 (27.18%), Postives = 88/195 (45.13%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIND-----TANSLSMKSSQIMNGDIVTCGVENNAT 853
++ G+ V + C + QG + W G LG +R++ YPR + G+ G++ L IE ++ D G++ECQ A R R +LTVL P P+I I + ++NLTC + N P + W +++G G + + D T ++L + +S I +G +TC N A
Sbjct: 32 IVAGEPVTLPCTIIGYQGMVLWVKDGLALGVNRDLSGYPRYDVIGDHSKGEYHLLIERTELVDDGSFECQAIQVAG---RSRPAQLTVLVPPDDPVI----------IGAPVVSLRAGDSLNLTCHANNAKPAASIIW------IRNGEVINGAAYTKTLLRDGKRESTVSTLHISASNIESGQQITCRASNKAA 207
BLAST of NPHS1-1 vs. Ensembl Zebrafish
Match: kirrel3a (kirre like nephrin family adhesion molecule 3a [Source:ZFIN;Acc:ZDB-GENE-091117-44]) HSP 1 Score: 81.6481 bits (200), Expect = 3.946e-15 Identity = 53/195 (27.18%), Postives = 88/195 (45.13%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIND-----TANSLSMKSSQIMNGDIVTCGVENNAT 853
++ G+ V + C + QG + W G LG +R++ YPR + G+ G++ L IE ++ D G++ECQ A R R +LTVL P P+I I + ++NLTC + N P + W +++G G + + D T ++L + +S I +G +TC N A
Sbjct: 32 IVAGEPVTLPCTIIGYQGMVLWVKDGLALGVNRDLSGYPRYDVIGDHSKGEYHLLIERTELVDDGSFECQAIQVAG---RSRPAQLTVLVPPDDPVI----------IGAPVVSLRAGDSLNLTCHANNAKPAASIIW------IRNGEVINGAAYTKTLLRDGKRESTVSTLHISASNIESGQQITCRASNKAA 207
BLAST of NPHS1-1 vs. Ensembl Zebrafish
Match: kirrel1b (kirre like nephrin family adhesion molecule 1b [Source:ZFIN;Acc:ZDB-GENE-070912-173]) HSP 1 Score: 77.7962 bits (190), Expect = 9.647e-14 Identity = 77/295 (26.10%), Postives = 124/295 (42.03%), Query Frame = 2
Query: 236 IHRIQC---FRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPI-DLKWNINEDDLMIAQDKILSVKSEH 1108
+HR+ F + P+ + V+ G+ V++ C V + G +QW G LG ++ +PR R+ +G ++L I + ++D YECQ + AA R R KLTVL P P+I I ++ ++ NLTCVS P+ T EW K G G S E ++D + +I D T N T NL + K P +++ IE V EG +++ +C +PPI +W L A++ + ++H
Sbjct: 18 VHRVFSGPRFSQEPADQ-SVVIGERVVLSCVVFNYTGIVQWTKDGLALGIGEDLRAWPRYRVLRIMDVGQYNLEITSADLTDDSLYECQATEAAL---RSRRAKLTVLIPPDGPVI-----EGSPEILLTA-----GTSFNLTCVSRGAKPMSTIEW------YKDGIIVEGAHTSTEVLSDRKRVTTKSFLEIQPMDTDTG---RNFTCVASNLAAPLGKRSTVTLNIHHPPTVIL----SIEPRS-VLEGERVKFTCQAT--ANPPIMGYRWAKGGVILDGARESVFETTADH 282
BLAST of NPHS1-1 vs. Ensembl Zebrafish
Match: kirrel1b (kirre like nephrin family adhesion molecule 1b [Source:ZFIN;Acc:ZDB-GENE-070912-173]) HSP 1 Score: 77.7962 bits (190), Expect = 1.004e-13 Identity = 77/295 (26.10%), Postives = 124/295 (42.03%), Query Frame = 2
Query: 236 IHRIQC---FRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPI-DLKWNINEDDLMIAQDKILSVKSEH 1108
+HR+ F + P+ + V+ G+ V++ C V + G +QW G LG ++ +PR R+ +G ++L I + ++D YECQ + AA R R KLTVL P P+I I ++ ++ NLTCVS P+ T EW K G G S E ++D + +I D T N T NL + K P +++ IE V EG +++ +C +PPI +W L A++ + ++H
Sbjct: 18 VHRVFSGPRFSQEPADQ-SVVIGERVVLSCVVFNYTGIVQWTKDGLALGIGEDLRAWPRYRVLRIMDVGQYNLEITSADLTDDSLYECQATEAAL---RSRRAKLTVLIPPDGPVI-----EGSPEILLTA-----GTSFNLTCVSRGAKPMSTIEW------YKDGIIVEGAHTSTEVLSDRKRVTTKSFLEIQPMDTDTG---RNFTCVASNLAAPLGKRSTVTLNIHHPPTVIL----SIEPRS-VLEGERVKFTCQAT--ANPPIMGYRWAKGGVILDGARESVFETTADH 282
BLAST of NPHS1-1 vs. Ensembl Xenopus
Match: NPHS1 (NPHS1 nephrin [Source:NCBI gene;Acc:100494620]) HSP 1 Score: 123.635 bits (309), Expect = 1.570e-27 Identity = 181/852 (21.24%), Postives = 322/852 (37.79%), Query Frame = 2
Query: 248 QCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPA-ASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTV--YVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMI--AQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDN----VRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTW------LVNKQPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQL----TVEWIR 2746
Q FR P V++G L+ C V H G +QW G +LG DRNIP + R MTG+ G+++L IE ++ D Y CQ+ + S R + VL P P + ++ ++ +TC + + P ++ +S L+ N S + + +TA + NG ++TC N A + T + M V + P I IE + V G L+L C+ + G+P L+W+ N+D + D+++ H + N N++ S N+ P + I++N + + T V++ S+ E + SC FT V+I W++ E + T + G++ ++E + ++ + ++ + KS + ++ + E P + G S K+ C +GG+P P+L + + S +H I E I P DN +RC S +AS++ L+V P + + IISS + C T SS P + I W + LG + + S L N ++ + F+S+ +N+ ++ K+ + +PP+FL+ P VQ VE G I ++ A+P YTW L+ + + +++ +++ A + Y++ N GK + L++ Y PT+ + + E I C+A ANP+ + + + WIR
Sbjct: 19 QVFRVQPD-NITVVQGSTALLSCEVEHGTGMVQWEKNGLLLGPDRNIPGFLRYSMTGDPHKGEYNLRIERSELDDDAEYHCQVGRSEVSLGIISRTALINVLIPPKAPTFEEYAAGSRVTWVYGVE-------YTVTCQATDAKPAAAL--TIKKSGLEVSGDSSSNPGSRDKLENTAMVAKVIPQSSDNGKLLTCLALNPALQ-----TPITVSFTMNV---LFPPNAPI-----IEGYIGPSVKAGETLKLICISLS-GNPLATLQWSKNDDVVSTRWETDEVVRASRSHLTLNIKPADNMA--------VVSCEAVNHVTPETLKTSIILN---------VVFLPTEVTILGSSS--GPEKSNLTFSC-----FTAASNPPVQIRWWLGPKEL-----VNTIVTISDAVHGGKVTMSNLTMEA----LREEHGMALTCEAFNEAILYTKSNSVTLSVQYPPQNVWIEAPPA----------DKFFRAGTSVKMTCFSSGGNPPPRLTWIKDTKSLSGGSQIHSGKIVTKEITIITVPSDNMATYRCNASNIGKTPALTASTK---LKVQFP--AIDVNIISSAKEYRRGSTIILTCVTGSSNPASTISWVKDGEQLKAQDLGRKPSLYGGISSSSRVTLKAASKDNGRRVSCEAFSSV-------------------------LNEAVNTFYKLNILFPPEFLDDQ--PAVVQ--AVEHGAAL-IPVRVRANPPQINYTWSLWGEKLIRDGAYRHHLRGEGALEIWNVSRADSGV---YNVTCVNKEGKNSIVIRLDVQYSPTIRSLGDTEVDLGSDAE------IACTADANPATDSMFQWRWLVWIR 759
BLAST of NPHS1-1 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 87.0409 bits (214), Expect = 1.258e-16 Identity = 63/204 (30.88%), Postives = 96/204 (47.06%), Query Frame = 2
Query: 254 FRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIND-----TANSLSMKSSQIMNGDIVTCGVENNA 850
F + PS K ++ GK V + C V +G +QW G LG +R++P + R + G+ +G+H+L IE V++SD YECQ + AA + R R LTVL P P+I + +N + NLTC++ P W + G K + E ++D +A+SL + S +G TC V N A
Sbjct: 26 FSQQPSDKV-IVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQATQAALRSQRAR---LTVLLPPGDPMIPGSPVANVL----------LNVPYNLTCLAPVAKPAAEITW------YRDGKKLDSAVYTKELLSDGKSEGSASSLLITPSLADSGSSYTCRVRNEA 209
BLAST of NPHS1-1 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 86.6557 bits (213), Expect = 1.643e-16 Identity = 64/204 (31.37%), Postives = 98/204 (48.04%), Query Frame = 2
Query: 254 FRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIND-----TANSLSMKSSQIMNGDIVTCGVENNA 850
F + PS K ++ GK V + C V +G +QW G LG +R++P + R + G+ +G+H+L IE V++SD YECQ + AA + R R LTVL P P+I S N+ +++ NLTC++ P W + G K + E ++D +A+SL + S +G TC V N A
Sbjct: 30 FSQQPSDKV-IVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQATQAALRSQRAR---LTVLLPPGDPMI---PGSPVANVLLNVP-------YNLTCLAPVAKPAAEITW------YRDGKKLDSAVYTKELLSDGKSEGSASSLLITPSLADSGSSYTCRVRNEA 213
BLAST of NPHS1-1 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 86.6557 bits (213), Expect = 1.739e-16 Identity = 64/204 (31.37%), Postives = 98/204 (48.04%), Query Frame = 2
Query: 254 FRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIND-----TANSLSMKSSQIMNGDIVTCGVENNA 850
F + PS K ++ GK V + C V +G +QW G LG +R++P + R + G+ +G+H+L IE V++SD YECQ + AA + R R LTVL P P+I S N+ +++ NLTC++ P W + G K + E ++D +A+SL + S +G TC V N A
Sbjct: 30 FSQQPSDKV-IVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQATQAALRSQRAR---LTVLLPPGDPMI---PGSPVANVLLNVP-------YNLTCLAPVAKPAAEITW------YRDGKKLDSAVYTKELLSDGKSEGSASSLLITPSLADSGSSYTCRVRNEA 213
BLAST of NPHS1-1 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 86.6557 bits (213), Expect = 1.915e-16 Identity = 63/204 (30.88%), Postives = 96/204 (47.06%), Query Frame = 2
Query: 254 FRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIND-----TANSLSMKSSQIMNGDIVTCGVENNA 850
F + PS K ++ GK V + C V +G +QW G LG +R++P + R + G+ +G+H+L IE V++SD YECQ + AA + R R LTVL P P+I + +N + NLTC++ P W + G K + E ++D +A+SL + S +G TC V N A
Sbjct: 38 FSQQPSDKV-IVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQATQAALRSQRAR---LTVLLPPGDPMIPGSPVANVL----------LNVPYNLTCLAPVAKPAAEITW------YRDGKKLDSAVYTKELLSDGKSEGSASSLLITPSLADSGSSYTCRVRNEA 221
BLAST of NPHS1-1 vs. Ensembl Mouse
Match: Kirrel (kirre like nephrin family adhesion molecule 1 [Source:MGI Symbol;Acc:MGI:1891396]) HSP 1 Score: 75.485 bits (184), Expect = 4.455e-13 Identity = 54/200 (27.00%), Postives = 87/200 (43.50%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVL------KVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIND-----TANSLSMKSSQIMNGDIVTCGVENNA 850
V+ G+ ++ C + + G +QW G LG + + +PR R+ G+A G ++L I + ++SD +YECQ + AA R R KLTVL ++ P+I + NLTC + N P T W + G + G S E + D T + L ++ + + G + TC N A
Sbjct: 64 VVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAAL---RSRRAKLTVLIPPEETRIDGGPVILLQA----------------GTPYNLTCRAFNAKPAATIIW------FRDGTQQEGAVTSTELLKDGKRETTISQLLIEPTDLDIGRVFTCRSMNEA 238
BLAST of NPHS1-1 vs. Ensembl Mouse
Match: Kirrel (kirre like nephrin family adhesion molecule 1 [Source:MGI Symbol;Acc:MGI:1891396]) HSP 1 Score: 75.0998 bits (183), Expect = 7.182e-13 Identity = 56/201 (27.86%), Postives = 89/201 (44.28%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVL------KVPSTPII-YQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIND-----TANSLSMKSSQIMNGDIVTCGVENNA 850
V+ G+ ++ C + + G +QW G LG + + +PR R+ G+A G ++L I + ++SD +YECQ + AA R R KLTVL ++ P+I Q G + NLTC + N P T W + G + G S E + D T + L ++ + + G + TC N A
Sbjct: 64 VVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAAL---RSRRAKLTVLIPPEETRIDGGPVILLQAG-----------------TPYNLTCRAFNAKPAATIIW------FRDGTQQEGAVTSTELLKDGKRETTISQLLIEPTDLDIGRVFTCRSMNEA 238
BLAST of NPHS1-1 vs. Ensembl Mouse
Match: Kirrel (kirre like nephrin family adhesion molecule 1 [Source:MGI Symbol;Acc:MGI:1891396]) HSP 1 Score: 75.0998 bits (183), Expect = 7.182e-13 Identity = 56/201 (27.86%), Postives = 89/201 (44.28%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVL------KVPSTPII-YQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIND-----TANSLSMKSSQIMNGDIVTCGVENNA 850
V+ G+ ++ C + + G +QW G LG + + +PR R+ G+A G ++L I + ++SD +YECQ + AA R R KLTVL ++ P+I Q G + NLTC + N P T W + G + G S E + D T + L ++ + + G + TC N A
Sbjct: 64 VVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAAL---RSRRAKLTVLIPPEETRIDGGPVILLQAG-----------------TPYNLTCRAFNAKPAATIIW------FRDGTQQEGAVTSTELLKDGKRETTISQLLIEPTDLDIGRVFTCRSMNEA 238
BLAST of NPHS1-1 vs. Ensembl Mouse
Match: Kirrel3 (kirre like nephrin family adhesion molecule 3 [Source:MGI Symbol;Acc:MGI:1914953]) HSP 1 Score: 73.1738 bits (178), Expect = 2.214e-12 Identity = 54/194 (27.84%), Postives = 83/194 (42.78%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIND-----TANSLSMKSSQIMNGDIVTCGVENNA 850
V+ G+ V + C + G + W G LG R++ +YP+ + GN + G+H L I ++ D YECQ AA R R +LTVL P PII +S++ + +NLTC + N P + W L+ G G S + D ++L + + NG + C N A
Sbjct: 26 VVSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVGNHLSGEHHLKILRAELQDDAVYECQAIQAAI---RSRPARLTVLVPPDDPIILGGP-------VISLRAGD---PLNLTCHADNAKPAASIIW------LRKGEVINGATYSKTLLRDGKRESIVSTLFISPGDVENGQSIVCRATNKA 200
BLAST of NPHS1-1 vs. Ensembl Mouse
Match: Nphs1 (nephrosis 1, nephrin [Source:MGI Symbol;Acc:MGI:1859637]) HSP 1 Score: 73.559 bits (179), Expect = 2.733e-12 Identity = 75/325 (23.08%), Postives = 128/325 (39.38%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEM 1258
V+EG V + C V +QWA G +LG + IP +PR + G++ G+ L IE +SD YECQ+ + P ++ +L + +P + Q ++ ++ +TCVS + P P + + N+ S E + T + NG ++ C N A IK + PG VI E +V G L+L C+ G+PP L+W N + IA + ++ + + M+ + + H L C A ++ + V +PPS + +
Sbjct: 57 VVEGSTVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQVGRSELGP--ELVSPSVILSILVSPKVLQLTPEAGSTVTWVAGQEYV-----VTCVSGDAKPAPDIIF-IQGGRTVEDVSSSVNEGSEEKLFFTEAEARVTPQSSDNGQLLVCEGSNPALATP-------IKASFTMNILFPPGPPVIDWPGLNEG--HVRAGENLELPCIARG-GNPPATLQWLKNGKPVSIAWG---TEHAQAVAHSVLVMT-VRPEDHGARLSCQSYNSVSAETQERSITLQVTFPPSAVTI 359
BLAST of NPHS1-1 vs. UniProt/SwissProt
Match: sp|Q9U3P2|SYG2_CAEEL (Synaptogenesis protein syg-2 OS=Caenorhabditis elegans OX=6239 GN=syg-2 PE=1 SV=1) HSP 1 Score: 121.709 bits (304), Expect = 3.260e-26 Identity = 259/1204 (21.51%), Postives = 457/1204 (37.96%), Query Frame = 2
Query: 443 LFIENVKVSDSGNYECQ-LSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDT---ANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQL-------RLLDERDREMKSSNLHEIGITRLEYNIEVKP----RDNEKKF---------RCIV--SYNNVMDFESASS----------------------------ELMALQVANPPKQLHMKIISSMDN-LIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMAN----YSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPT-RPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESL--DNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYI-CVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDT----VVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSREK 3853
L I +V + D G YECQ L P + P R + L ++ VP + + N Q N SI + ++ +N+TCV N P P W ++ + K + S +N T SL ++S + +G ++TC + F I T + P E+ + + G + ++C G+PP D+ W ++ + L +S+ +N + S+ + E C + P + + VNYPP+ +E+ ++N+ + + + CK S +I W I + + T ++ +G I SS V + + +H ++ V C+ + E + +Q I++ P P++T L + G+ + C GG+P +L + L + ++MK S + + + L EV R ++ F C V ++N V F S S+ E L V PP+++ ++ S D L+ ++ + C T SS P A I W + R+T + L + ++ + +FT T E+D + + C I H +W ++ + V YPP+ L ND T V+ +G++F + +P+I+ + W N P + H V V + ++++ Y+L N++G L + Y + S I ++ C A PS+ + + W + + E L +G +K I +R T Q + G Y C N P +T LV R PV + K+ A + G ++ C + VPTV+++ + K+E L N KY Q + +++ L I + D K + C+A N +G + + P P +P N T+ N T ++SV W RF+GGS Q ++ +R ++ +VN + N + + FF I S+N ++ P+I T+N + G N + R E ++ ++ IL+ V +LL + +I FV R K RK REK
Sbjct: 89 LKITHVNLDDDGEYECQMLHP--EEGPIRAKSFLNII-VPPQLVYFSNYQPN------SIIAVKENTPLNITCVVPNVKPEPEVLWYMDGKVMSRDVK----QASTPHLNKTFTVYTSLVVQSDRNDHGKVITC--------EAFQKETDIRITTNTTLDVLFPPSDPTVEI--LRNPSALRSGDNVTIACSVTG-GNPPPDVFWY--HENKRLQSHSTLDTRSKEI-KNIYSFI-ASQNDNMAEYECRANNSRTGNPKRKAMKLEVNYPPASVELFG---ESNIRYGSSANIQCKSLPSNPAS-----------QITWIING------RSVPTPTQREFVVENG----IVSSSNVSVHSNELSVEAHQIN-----VECMATNPEGSSA--KQHVIKIIAPPKAPIIT--GLEDRKFF-EGDIVNVTCEAQGGNPLAELSWYRGSEKHLVKGRKKMKWSQVKKKLVMSLLCLCEVVIMNFFRFSKNVFYFFRFTLFIECGVNGAHNEVAGFSSYSTLALRVDRTMNTQRLKCEATNAALDEPLIESQYLSVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFS-----------RATDDNKVHLGDVSLNETTRDNGFNVENVLSFTP-----TEEYDGTVVHC-IANHPEW---KHSVNTSFPLNVMYPPKML-----VNDPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFD---HTIGRVFARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAAT-----GDTVLLECEADGEPSKANM-IHWYK------NGEILAAQHRGHKKAI----LRLNATEQQS------------------------------GEYTCKADNGIGVPAEGQTFLLVNSAPRVLPVARYAKT--AGILGGVAKARCKV--YAVPTVEFV-WEKDEQLIKKNSSKYSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKYRRQNDDLIHLVNTTHTNLRLSGLATANTYFFQIR-------------SINARGFASSWTSPVIFATLN---------------EDGVNVAM-IRNEKLKLTTWIPYILIFIVLFMLLNV---VICCFVCNRT-KKRKLREK 1118
BLAST of NPHS1-1 vs. UniProt/SwissProt
Match: sp|O60500|NPHN_HUMAN (Nephrin OS=Homo sapiens OX=9606 GN=NPHS1 PE=1 SV=1) HSP 1 Score: 81.2629 bits (199), Expect = 6.902e-14 Identity = 132/560 (23.57%), Postives = 211/560 (37.68%), Query Frame = 2
Query: 254 FRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQP----PRRRMTKLTVLKV------PSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRL-----------LDERDREMKSSNLHEIGIT-RLEYNIEVKPRDNEKKFRC 1867
F LP V+EG V + C V+ +QWA G +LG D IP +PR R+ G+ G+ L IE +SD YECQ+ + P PR ++ L K+ T + + GQ N K D I L+G + +VN G++ + T+ TA ++ +SS N ++ C + A E IK V PG VI+ E +V G L+L CV G+P L+W N + A + ++ +++ M+ + + H +L C A + + V +PPS I + + +T E + V LSC S + V + W++ W+ + E+TV S+L D ++ + C S F E ++ I + P+ L + + Q L G +L C GG+P P L L + R + ++ + G T E + P DN+ KF C
Sbjct: 34 FWALPE-NLTVVEGASVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVGRSEMGPELVSPRVILSILVPPKLLLLTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITI-----LLSGQTISDISANVN----------EGSQQKLFTVEATAR-VTPRSSD--NRQLLVCEASSPA-------LEAPIKASFTVNVLFPPGPPVIEWPGLDEG--HVRAGQSLELPCVARG-GNPLATLQWLKNGQPVSTAWG---TEHTQAVARSVLVMT-VRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSASQT-------------ENKNVTLSCVSKS-SRPRVLLRWWL------GWRQL-LPMEETVMDGLHGGHISMSNLTFLARREDNGLT----------LTCEAFSEAFTKETFKKSLILNVKYPAQ-KLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESRLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTC 528
BLAST of NPHS1-1 vs. UniProt/SwissProt
Match: sp|B1Q236|SYG1_CAEEL (Synaptogenesis protein syg-1 OS=Caenorhabditis elegans OX=6239 GN=syg-1 PE=1 SV=1) HSP 1 Score: 77.7962 bits (190), Expect = 7.216e-13 Identity = 49/140 (35.00%), Postives = 74/140 (52.86%), Query Frame = 2
Query: 293 GKDVLMGCRVAHQQGKLQWAFKGKMLGFDRN--IPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPA-ASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSV 703
G+ ++ CRV HQQG +QW LG DR+ +P R RM G+A G+++L I NV + D ++ CQ+S + ++ KLTVL P+ P I ++ S K +I D I +C+S G P PT W++
Sbjct: 37 GETAILTCRVEHQQGPVQWMKDDFGLGTDRDKPLPGNKRYRMVGSAANGEYNLEISNVTLFDDDDFACQISESDHAKAVVSSKAKLTVLVRPTPPKIVKSHHSLK-----AIAGDP----ITQSCLSRKGKPPPTIGWAI 167
BLAST of NPHS1-1 vs. UniProt/SwissProt
Match: sp|Q08180|ICCR_DROME (Irregular chiasm C-roughest protein OS=Drosophila melanogaster OX=7227 GN=rst PE=1 SV=2) HSP 1 Score: 76.2554 bits (186), Expect = 2.264e-12 Identity = 78/319 (24.45%), Postives = 124/319 (38.87%), Query Frame = 2
Query: 293 GKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMT--KLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEW------------SVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERI-IKTQMRVFKRI---LPGKIVIKEVSDIEKTVYVTEG------HQLQLSCVCN-NPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYC 1174
G V + CRV ++QG LQW LG R++ + R M G+ GD+SL I V + D Y+CQ+SP P R T LTVL P P I Q + D + + CVS+ G P W PL +F V L + + + +C +N A R + R+ +K +V + LPG +V+++ G Q++L C + NP D + +W IN++ ++ Q + ++ N++RK+H + C
Sbjct: 42 GARVTLPCRVINKQGTLQWTKDDFGLGTSRDLSGFERYAMVGSDEEGDYSLDIYPVMLDDDARYQCQVSPGPEGQPAIRSTFAGLTVLVPPEAPKITQG----------DVIYATEDRKVEIECVSVGGKPAAEITWIDGLGNVLTDNIEYTVIPLPDQRRFTAKSV-----------LRLTPKKEHHNTNFSCQAQNTADRTYRSAKIRVEVKYAPKVKVNVMGSLPGGAGGSVGGAGGGSVHMSTGSRIVEHSQVRLECRADANPSD--VRYRWFINDEPIIGGQKTEMVIR------------NVTRKFHDAIVKC 325
BLAST of NPHS1-1 vs. UniProt/SwissProt
Match: sp|Q96J84|KIRR1_HUMAN (Kin of IRRE-like protein 1 OS=Homo sapiens OX=9606 GN=KIRREL1 PE=1 SV=2) HSP 1 Score: 75.0998 bits (183), Expect = 3.979e-12 Identity = 56/201 (27.86%), Postives = 88/201 (43.78%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVL------KVPSTPII-YQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIND-----TANSLSMKSSQIMNGDIVTCGVENNA 850
V+ G+ ++ C + + G +QW G LG + + +PR R+ G+A G ++L I + ++SD +YECQ + AA R R KLTVL ++ P+I Q G + NLTC + N P T W + G + G S E + D T + L + + + G + TC N A
Sbjct: 32 VVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAAL---RSRRAKLTVLIPPEDTRIDGGPVILLQAGTPH-----------------NLTCRAFNAKPAATIIW------FRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEA 206
BLAST of NPHS1-1 vs. TrEMBL
Match: A0A0H3YFQ1 (NPHS1-1 OS=Schmidtea mediterranea OX=79327 GN=NPHS1-1 PE=2 SV=1) HSP 1 Score: 3360.47 bits (8712), Expect = 0.000e+0 Identity = 1621/1621 (100.00%), Postives = 1621/1621 (100.00%), Query Frame = 2
Query: 77 MLNFWMICVFVLSNTFINRIEFVLCQDPFNVDDLDAAESISLSQSSTCTEKDDIHRIQCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSREKLSSPPTHQRMDTVGFISNDDSIEMRPPSLVRSGIESNLYAQSISDHMSQNGSDTGLKLNYYKTHSDDQFSSPFLGPELDLVASVRGRNNYDINALSPNFHPGYIQFAESSSMTSAQDPYIKGYYTQRPYYNSQTPSHGVIRKSSHSSNDTVTLPQYPISSSYQKRMDKVCFPQSAVSISTNSGDSNCSQSPPRKYIGYSPMVNQSGAPSTVEEYLSSGNSASESHRFGINGSDEYFTSPMSSFRLNQGLDSMSNKDFYSQLCSTNQQNQESAQEFAIQRIMYGNDVSAITPYRAYNDHQRLSTSSDNEHLLKPPLDFSGIESNNSNNIQSPMADAHKLSYVDLIAHSSPSVIRFTGPISENL 4939
MLNFWMICVFVLSNTFINRIEFVLCQDPFNVDDLDAAESISLSQSSTCTEKDDIHRIQCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSREKLSSPPTHQRMDTVGFISNDDSIEMRPPSLVRSGIESNLYAQSISDHMSQNGSDTGLKLNYYKTHSDDQFSSPFLGPELDLVASVRGRNNYDINALSPNFHPGYIQFAESSSMTSAQDPYIKGYYTQRPYYNSQTPSHGVIRKSSHSSNDTVTLPQYPISSSYQKRMDKVCFPQSAVSISTNSGDSNCSQSPPRKYIGYSPMVNQSGAPSTVEEYLSSGNSASESHRFGINGSDEYFTSPMSSFRLNQGLDSMSNKDFYSQLCSTNQQNQESAQEFAIQRIMYGNDVSAITPYRAYNDHQRLSTSSDNEHLLKPPLDFSGIESNNSNNIQSPMADAHKLSYVDLIAHSSPSVIRFTGPISENL
Sbjct: 1 MLNFWMICVFVLSNTFINRIEFVLCQDPFNVDDLDAAESISLSQSSTCTEKDDIHRIQCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSREKLSSPPTHQRMDTVGFISNDDSIEMRPPSLVRSGIESNLYAQSISDHMSQNGSDTGLKLNYYKTHSDDQFSSPFLGPELDLVASVRGRNNYDINALSPNFHPGYIQFAESSSMTSAQDPYIKGYYTQRPYYNSQTPSHGVIRKSSHSSNDTVTLPQYPISSSYQKRMDKVCFPQSAVSISTNSGDSNCSQSPPRKYIGYSPMVNQSGAPSTVEEYLSSGNSASESHRFGINGSDEYFTSPMSSFRLNQGLDSMSNKDFYSQLCSTNQQNQESAQEFAIQRIMYGNDVSAITPYRAYNDHQRLSTSSDNEHLLKPPLDFSGIESNNSNNIQSPMADAHKLSYVDLIAHSSPSVIRFTGPISENL 1621
BLAST of NPHS1-1 vs. TrEMBL
Match: A0A0H3YFQ6 (NPHS1-6 OS=Schmidtea mediterranea OX=79327 GN=NPHS1-6 PE=2 SV=1) HSP 1 Score: 385.956 bits (990), Expect = 5.410e-106 Identity = 376/1510 (24.90%), Postives = 673/1510 (44.57%), Query Frame = 2
Query: 71 NEMLNFWMICVFVLSNTFINRIEFVLCQDP---FNVDDLDAAESIS---LSQSSTCTEKDDIHRIQCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPP-RRRMTKLTVLKVPSTP-IIYQNGQSNKQNISV-SIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNI----------NEDDLMIAQDKILSVKSEHFSQN----TFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPS---KIEMEAISIKT-NVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIK----------SSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNV-MDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFD--LSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKK-IH-IDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLG---ASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVN-----------------------VNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEP--VESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSR--EKLSSPPTHQRMDTVGFISNDDSIEMRPPSLVRSGIESNLYAQSISDHMSQNGSDTGLKLNYYKTHS------DDQ--FSSPFLGPELDLVASVRGRNNYDINALSPNFHPGYIQFAESSSMTSAQDPYIKGYYTQRPYYNSQTP---SHGVIRKSSHSSNDTVTLPQYPISSSYQKRM 4351
N L F+++ + + S FV Q P F+ DL +E S + QS CT ++ ++RIQCF+E+P P Y+V +G + C V +Q GK+QW +LG+DRNIP P R+ G+ V G+H L I NV V D+G YECQ+ P + P RR TKL VL P P I Y+ Q N + + SIK+ D TCVS NG P F+W +N + ++ N +E +N + + LS+K+S+ +GD++ C V N AT + R + T++ + PG+ I +++ + T YV + + + ++C + G P L W N + +D + S+N T + ++ K H +L+C + + S+A L +++ P + ++S +T N + +Y + G++ ++CT+D ++ + I W S E WK E F + +K I + + SF+D S I S ++ ++K+ + P P + N+ KP L + + ++ C+ GG+P P+L+ + + ++SS + + + I + P N+ K + ++ + +S+ + L V PP + +K +S + + + ++QC S P A + W + + LG R V++ C E+L+ + + ++ P + L +++C + + + ++VL K I+I V + PQF + F +E+G + ++ ++ IA+P I YTW N + +K +H I TS K I NA+P + NY+L+V N+ G++ +LNITY P ++G +E I+ N ++E CS Q+NP+ ++ +W +K++ + DI + + I C + ++K N C+ E+ + + I +D +G YRC++ N R +KTI+L P + + S+ A + + LFCY P P + W N S+ KY ++ + G++ A L I + +D G Y+C A N G S + ++ +P P+ V+N +++ + + WI F+GGS Q ++ W+ + Y+ ++ D+ K F I L + Y ISI S N ++ K I+ TM+ D+++ N+ I+P ++ N L I+ + G ++L + I +I++ +R+R+ + R ++ + ++P + +S DDS++ + +RS + LY + + S L N ++ HS DDQ FSS G + +R + + + PN G S+ A DP Y S TP HG+ + DT++ PI Q+++
Sbjct: 7 NCFLKFFLVLILIGS--------FVSSQHPDQSFDDSDLTDSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIINSIKQ---DVRTEFTCVSDNGQPKVKFQWFLNNNLIQDN----ENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDN-IIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNE-ATIKWVFSPNPAINNDFISPVEKIWK------EDESFRRNSTIK-IKINKQFDQSFLDCQAWSGIKS------------------VQSRVKLHITYSPEKPTIQGYNVGKPVKLNH--NFEMTCSTIGGNPYPELQWFRD-SKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAE-VENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRP----VQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVF-NPDYGEEHQVLRKRIQINVTFSPQFEGIINY-----NFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELE--------CSVQSNPANPHMSYKW----TKLVPGKGYNDI--SSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNG-IRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPT--SLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSA------------DNEKENNQPTYPIIQPNKLQDNTILFIVAASVFGFVILVVNILVIIFVLRRRRQRSRTAKLEARYNNPKSLLAASNGLILSRDDSLKDSNFNYLRSDM-VELYPRGLD-------SSQILHHNPHQIHSLRRSIPDDQKTFSSFETGSDDAREDLLRRQKEFGCCGIDPNSQKG--------SIIVAYDPITNSSYYAPANATSPTPIYLQHGMYDQ------DTMSTLLSPIHEGGQRQL 1409
BLAST of NPHS1-1 vs. TrEMBL
Match: A0A4E0RLR1 (Nephrin OS=Fasciola hepatica OX=6192 GN=D915_000783 PE=4 SV=1) HSP 1 Score: 374.785 bits (961), Expect = 1.131e-101 Identity = 352/1446 (24.34%), Postives = 594/1446 (41.08%), Query Frame = 2
Query: 200 LSQSSTCTEKDDIHRIQCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQ-NGQ---SNKQNISVSIKKDEFDSN--INLTCVSLNGNPVPTFEW-----SVNRSPLKHGFKF-----RGNKVS--IETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVS-DIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDK--------------ILSVKSEHFSQ--------NTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEM----EAISI---------------KTNVSLHNPSTV--YCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYI---------TSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDER-DREMKSSNLHEI------------GITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLI-------------------------------ANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTF------TSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIK-------IRVRY------------PPQFLN-----------VHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDK-----FSIVNAQP---EMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKIL------------------------DQEELLDILKGQQKFISSDPIRCTNTHQWNKG-KVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPN--PVKNFRKS----------------EFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGD-YQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSK-RGF---------------NKQLEKR-------IEPVESNNFLIIILLATVGVLLLTIKIGLIVYFV-RKRKLKHRKSREKL 3856
+ QS+TCT + D RIQC RELP+ Y+VLEG+DV M CRVAHQ+GK QW + +LG+ RNIP++PR M G+ G+H L I NV D+G YECQ+SP A Q P RR T L++L PS P+I +G+ + ++V ++ + L+C S GNP P FEW +N++ F RG+ + I + + L + Q+ GD + C + N AT++ L+++ ++ ++ V PG I + S + +TEG L+ +C + G+PP +L W A+ K I + +E+ Q + R++ ++ H +L C+ + SA + + V Y P +++ E + + + N N ++ Y + + L C + +F D +I W+ + W PI T + G+ +I T+S P + S + + V E N + + + ++ + P P +++ + + G + L C G P L L+ + H + G+ R I++ N ++C E S+ + + VA + M + + A ++CH D S P KI+W + + L + + KD+ C E + Q I Y+ + ++ F + I + L IEC+ P W +S IK +R R P Q LN H+LP F+V G + ++ A+P + + W N++ + S D+ S++ +P MANY+LL N+LG A F FFLN+TYGP L+G+ I V KA++ C A+ANP+ + V W R IS Q+ + D +KG IRC N W+ G K C + + +++ I + +G Y+C + N+ P A++ + L+YP P PV + KS + +A +F ++ L C P PTV+W+ N +L ++ + + PG + AVL ++ + DLGKY C A N GE + V L P +P P ++N T T+++V W +NGGS Q+ KL W + N D + A+I ED +++ + I L + Y +++ + N ++G + ++ T DP PD + ++++ + F N + ++R ++ E +III + G L+L + +I + R RK H++ R +
Sbjct: 109 IDQSNTCTPQQDAQRIQCLRELPAESYEVLEGEDVQMRCRVAHQRGKAQWRARHYLLGYKRNIPSWPRYSMQGDPRQGEHDLVITNVTHEDAGTYECQVSPVAGQSPLRRQTTLSILVKPSQPVIVAPSGEPQPTPNGRLTVPLRHSTPGERNLLRLSCQSHGGNPPPIFEWFHNGLVLNKTTTSSDTDFLTNTGRGDDFAFRIPGVEYHSAELILDKEQLRTGDRLVCLISNKATQRSKKLSQQKLRAELYVIIHSPPGNPEIIDWSLKSNASQILTEGAPLEATCRSSPAGNPPGELVWRWESSSSDSAEGKASLTPNPVTRIPQIITPIPAEYAVQQHMDEKLLSKVRITQVNSSQHGMDLVCA-VKHPIGPEKSARIPVFVRYGPQSVDIHGPEETVDLLKEAMDKRLHLFQTDRDNRGTRNATSAIRYVYQDRPQYLLCLTSPYF-DRAQIQWYGLLNISGQWHPIAPLESFTRVLATGQKNFILASMIQLVSTNSERTPLRWTAIKCVSKADVTDAAGIHKDVSDLESNKAVMKIIDLKSYVPPGKP--SISGYKQNMLYQEGTNITLSCEARAGIPPGYLVWLESSVAKASAEKTRHSLVIPNTTTLAGPDGMIRSTLIIQLTREQNGHFYQCASENKGFPRSEVRVSDSVQMGVAFAATSITMTVADETGRALFPRDHTLSQNSNPNQSVRLDKSHNPPIVSAQAGGKVAVKCHMDLSNPRPKIQWRLYRCPTHTVNLSEQISFKDLTKDCVIETI---QGIIYRGTEENVFDEKVIESQILLALNWSHHGDIIECVAEPQVPWAMGPDQVSVHIKDGLSQPRLRERVLMNILFAPLFLRPAQHLNWPVEKMHVGLFEHRLPQ----FIVVDGGAVDLDLEPSANPEVTSFVWYKNEEMLSTSAATSGSFDERISVHHSVLRIKPVRVADMANYTLLATNSLGTARFRFFLNVTYGPQLIGSPILNITVA-----GRKALLECQAKANPTPSENAVRW-RRISHSFAHLDPVHKFYRASNNAVTMSESNEQQQSIADSVKG---------IRC-NKGTWHNGFKYFAKCWNPSPGVLASSLTIYDLGPSDVGRYQCHMDNAVGSP-AVRIVDLIYPFAPQVVPVPRWTKSAPGKGYEGLVNNSSDEDPSAGVF-RARLTCVIAAEPKPTVEWIREPANLTLVEGGQFRSQIKAVRPGLYHAVLFLNKPRDVDLGKYYCKAKNLVGEDAGQVELGTPTAPDLPTKPRLLNATSTTLAVAWTQGYNGGSLQKFKLRWASNE---NPDKFTEADISEDLDRETIAYEIKGLQKITSYRVAVSASNAEHGTSGFTQYLLASTTAR-DPIPDKEAMHDEARVKSFSTLGHWDTKGSGPDDNDESQRRLARAGLDVQTSEDKAVVIIIAASIFGSLVLVANLLIIAFLAQRHRKKLHQQRRSSI 1521
BLAST of NPHS1-1 vs. TrEMBL
Match: A0A4S2LBK4 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008491 PE=4 SV=1) HSP 1 Score: 358.992 bits (920), Expect = 4.235e-97 Identity = 326/1328 (24.55%), Postives = 584/1328 (43.98%), Query Frame = 2
Query: 170 DDLDAAESISLSQSSTCTEKDDIHRIQCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSP--AASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRS-----PLKHGFKF---RGNKVSIETINDTA-----NSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWN--------------------------INE-------------------DDLMIAQDKILS--VKSEHFSQN--------TFRMSNISRKWHRKELYCSGSQDNYAIPSS--ATVLILVNYPPSK--IEMEAISIKTN-VSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKT--VFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVK--PR--DNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDV------------RSLCQSEIL--NEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKK---------IHIDTSVDKF-SIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWM----TFYKNESLDNPV---KYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVR 3817
D +DAA I QSS CT ++ RIQCF LP +Y V G V M C V +Q GK+QW K +LG+DR++P YPR R+ G+ G H+L I +V++ D+G YECQ++P A + P RR T+L VL PS P I N + + D I + C ++ G+P P+F W +N PL+ RG ++ I +T + LS+ + + +GD +TC V N AT+ ++ +R + TQ+ + PG I +S K Y+ EG +L+ CV + PG+P L W +NE D++ ++S V+ H++ N T + I+R++H +L C + + + S+ ++ I V Y P+ I ++ S++ VS S VY + G+ L C + +F I W +SS+ KP F +T + T DG+ S ++V ++D ++ R+ S E ++ + ++++++ P VPV+T +P + + S ++ C +GG+P P+L RD++ + N E+ + + ++++K P+ DN + C + N +S+ + L V PP+ + + + + Q + C DSS P A I WW H C +H SK R+ C+SE L E + + + ++ T + + +IEC E V +++ + + PQFL + V + +G ++ + ++P + +W V +P+ + +H + + ++ MANY+++ N++ +++ FFLN+T+ +L GA++E + + + ++C A ANPS W R + + D+ + + + + P ++ K ++C + + + + I I+ +G+Y C I N P K I VY P+ V+ R ++ A + L C P P + W+ Y ++ + K+ T + PG +K+ L +++ ++D G Y C A N GE+ V L+ +P P+N ++N T S+ V W F+GG Q ++ WRL T N ++ A++ ++ + I L EY++S+ S+N ++ I ++T + D +P S + S G P + N LII+ G ++L I + ++ + V+
Sbjct: 36 DSVDAARVI---QSSACTPRERQDRIQCFDVLPQDQYSVPLGNTVNMQCVVLNQHGKVQWRAKKILLGYDRSLPGYPRYRIIGDVGRGQHTLQIMDVQLEDAGEYECQVTPVPANNHPLLRRKTELVVLVKPSVPRILYPDVPPPDNQLIISQPDPI-VRITVLCATVGGSPAPSFTWIINNQEIPMKPLQASAGRHPDRGPWAKLDPIINTETEKQESRLSLLKAGLKDGDQLTCSVTNAATQLHHDIQQRNLTTQVVIRVHTPPGPPRI--ISPETKYEYL-EGEELKAVCVASPPGNPFGGLFWRWLLQPSQVGDQKTGRIIGFGGRGGARMNEYSDVEAYLKTAEHLGSQSADEMSQGMSDLISEDVQPLHYTLNKEKDQLINTLVIPRITRRYHAAKLIC---ETGHPVGSAHQTSIEIFVKYAPANVTISVDGGSVREELVSGRRESVVYGQAGEIKTLICRTSPYF-KQANIKWITQSSDSGPMKPPRQLFGQTKIMRTPDGKSWLQESRVDV--KISEEDDRGYVDCRVWS---------EGDLRVDARVRMDIIYPPGVPVITGYRSLQPVKMAH--SLEMTCVTSGGNPPPELAWF--RDKQ-QLPNAEEVILLGRQSSLKLKLVPQRDDNGAHYHC-AARNAATGNVGIASDAVVLNVVFPPQGVSVNF-HGPSVVPSGQPLVISCQADSSNPVATINWW-HFRCGPAHAFSQFKASKTTIEEALYDRRTSERAKCKSEKLEGTEENPVPGTAGGLKAQSRLWLRPTWHYHMDYIECRT---ENPEYGPPVWHDRLQLNITFAPQFLGLQTRKEQV----IREGTTVNLDLGPYSNPPVTSVSWFVEGRPIPPETPPAAARTGVHTSGRLGELLALHKISRTHMANYTVVARNDIAESSAVFFLNVTFPASLAGAAEENVT----QTTSDQVTLVCQATANPSIATQAFTWYRYVPPPGWPQ---DVNEAEVQLSAPMPCNQSDASPSVDDKYFIHCQRDDSIHMTSRLSIYRINRDDVGTYVCEINNGLGDP-VRKNIHFVYHFTPSIVRLPRYTKAAGEQGSTVTLTCLVRTEPTPQIGWLKAGQMIYDSQREGSGALTQKHQTQFLRIRPGLYKSTLTLTNLQKTDFGVYQCQAVNIKGEAMLEVSLSGTSTPDVPLNLRLLNSTANSLRVAWTQGFDGGLTQTFQVRWRLA-TGSNNMFKYADVAANDNHHAVEYLITGLESGAEYQVSVNSMNAKHGASAHTDFIHVRTEAF-DREPGSPLVPPLSSAG----------PSDDNALLIIVAACVFGFIVLIINLVVVTFLVK 1306
BLAST of NPHS1-1 vs. TrEMBL
Match: A0A4S2LB20 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008491 PE=4 SV=1) HSP 1 Score: 358.992 bits (920), Expect = 4.308e-97 Identity = 326/1328 (24.55%), Postives = 584/1328 (43.98%), Query Frame = 2
Query: 170 DDLDAAESISLSQSSTCTEKDDIHRIQCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSP--AASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRS-----PLKHGFKF---RGNKVSIETINDTA-----NSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWN--------------------------INE-------------------DDLMIAQDKILS--VKSEHFSQN--------TFRMSNISRKWHRKELYCSGSQDNYAIPSS--ATVLILVNYPPSK--IEMEAISIKTN-VSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKT--VFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVK--PR--DNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDV------------RSLCQSEIL--NEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKK---------IHIDTSVDKF-SIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWM----TFYKNESLDNPV---KYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVR 3817
D +DAA I QSS CT ++ RIQCF LP +Y V G V M C V +Q GK+QW K +LG+DR++P YPR R+ G+ G H+L I +V++ D+G YECQ++P A + P RR T+L VL PS P I N + + D I + C ++ G+P P+F W +N PL+ RG ++ I +T + LS+ + + +GD +TC V N AT+ ++ +R + TQ+ + PG I +S K Y+ EG +L+ CV + PG+P L W +NE D++ ++S V+ H++ N T + I+R++H +L C + + + S+ ++ I V Y P+ I ++ S++ VS S VY + G+ L C + +F I W +SS+ KP F +T + T DG+ S ++V ++D ++ R+ S E ++ + ++++++ P VPV+T +P + + S ++ C +GG+P P+L RD++ + N E+ + + ++++K P+ DN + C + N +S+ + L V PP+ + + + + Q + C DSS P A I WW H C +H SK R+ C+SE L E + + + ++ T + + +IEC E V +++ + + PQFL + V + +G ++ + ++P + +W V +P+ + +H + + ++ MANY+++ N++ +++ FFLN+T+ +L GA++E + + + ++C A ANPS W R + + D+ + + + + P ++ K ++C + + + + I I+ +G+Y C I N P K I VY P+ V+ R ++ A + L C P P + W+ Y ++ + K+ T + PG +K+ L +++ ++D G Y C A N GE+ V L+ +P P+N ++N T S+ V W F+GG Q ++ WRL T N ++ A++ ++ + I L EY++S+ S+N ++ I ++T + D +P S + S G P + N LII+ G ++L I + ++ + V+
Sbjct: 36 DSVDAARVI---QSSACTPRERQDRIQCFDVLPQDQYSVPLGNTVNMQCVVLNQHGKVQWRAKKILLGYDRSLPGYPRYRIIGDVGRGQHTLQIMDVQLEDAGEYECQVTPVPANNHPLLRRKTELVVLVKPSVPRILYPDVPPPDNQLIISQPDPI-VRITVLCATVGGSPAPSFTWIINNQEIPMKPLQASAGRHPDRGPWAKLDPIINTETEKQESRLSLLKAGLKDGDQLTCSVTNAATQLHHDIQQRNLTTQVVIRVHTPPGPPRI--ISPETKYEYL-EGEELKAVCVASPPGNPFGGLFWRWLLQPSQVGDQKTGRIIGFGGRGGARMNEYSDVEAYLKTAEHLGSQSADEMSQGMSDLISEDVQPLHYTLNKEKDQLINTLVIPRITRRYHAAKLIC---ETGHPVGSAHQTSIEIFVKYAPANVTISVDGGSVREELVSGRRESVVYGQAGEIKTLICRTSPYF-KQANIKWITQSSDSGPMKPPRQLFGQTKIMRTPDGKSWLQESRVDV--KISEEDDRGYVDCRVWS---------EGDLRVDARVRMDIIYPPGVPVITGYRSLQPVKMAH--SLEMTCVTSGGNPPPELAWF--RDKQ-QLPNAEEVILLGRQSSLKLKLVPQRDDNGAHYHC-AARNAATGNVGIASDAVVLNVVFPPQGVSVNF-HGPSVVPSGQPLVISCQADSSNPVATINWW-HFRCGPAHAFSQFKASKTTIEEALYDRRTSERAKCKSEKLEGTEENPVPGTAGGLKAQSRLWLRPTWHYHMDYIECRT---ENPEYGPPVWHDRLQLNITFAPQFLGLQTRKEQV----IREGTTVNLDLGPYSNPPVTSVSWFVEGRPIPPETPPAAARTGVHTSGRLGELLALHKISRTHMANYTVVARNDIAESSAVFFLNVTFPASLAGAAEENVT----QTTSDQVTLVCQATANPSIATQAFTWYRYVPPPGWPQ---DVNEAEVQLSAPMPCNQSDASPSVDDKYFIHCQRDDSIHMTSRLSIYRINRDDVGTYVCEINNGLGDP-VRKNIHFVYHFTPSIVRLPRYTKAAGEQGSTVTLTCLVRTEPTPQIGWLKAGQMIYDSQREGSGALTQKHQTQFLRIRPGLYKSTLTLTNLQKTDFGVYQCQAVNIKGEAMLEVSLSGTSTPDVPLNLRLLNSTANSLRVAWTQGFDGGLTQTFQVRWRLA-TGSNNMFKYADVAANDNHHAVEYLITGLESGAEYQVSVNSMNAKHGASAHTDFIHVRTEAF-DREPGSPLVPPLSSAG----------PSDDNALLIIVAACVFGFIVLIINLVVVTFLVK 1306
BLAST of NPHS1-1 vs. Ensembl Cavefish
Match: kirrel3a (kirre like nephrin family adhesion molecule 3a [Source:ZFIN;Acc:ZDB-GENE-091117-44]) HSP 1 Score: 75.485 bits (184), Expect = 3.436e-13 Identity = 54/195 (27.69%), Postives = 86/195 (44.10%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIND-----TANSLSMKSSQIMNGDIVTCGVENNAT 853
V+ G+ V + C + G + W G LG +R++ YPR + G+ G++ L I+ ++ D G++ECQ A R R +LTVL P P+I I I +NLTC + N P + W +++G G S + D T ++L + +S I +G +TC N A
Sbjct: 40 VVAGQPVTLPCSITGFHGMVLW--DGLALGVNRDLSGYPRYDVIGDHSKGEYHLLIQRTELGDDGSFECQGIQAGI---RSRPARLTVLVPPDDPVI----------IGAPIVSLRAGDPLNLTCHADNAKPAASIIW------IRNGEVLNGAMYSKTLLRDGQRESTVSTLYISASNIESGQRITCRASNKAA 213
BLAST of NPHS1-1 vs. Ensembl Cavefish
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:HGNC Symbol;Acc:HGNC:23204]) HSP 1 Score: 74.7146 bits (182), Expect = 7.274e-13 Identity = 55/194 (28.35%), Postives = 85/194 (43.81%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIND-----TANSLSMKSSQIMNGDIVTCGVENNA 850
V+ G+ V + C + G + W G LG R++ YPR + G+ G+H L I+ V++ D +ECQ AA R R +LTVL P P+I VS++ + +NLTC + N P + W +++G G S + D T ++L + S I G + C N A
Sbjct: 11 VVAGQPVTLPCSIPGYHGVVLWIKDGLALGVGRDLSGYPRYTVVGDHSSGEHHLRIQRVELMDDAVFECQAIQAAM---RSRPARLTVLVPPEDPVI-------DGGPIVSLRAGD---PLNLTCHADNAKPAASIIW------IRNGEVLNGAMYSKTLLRDGRRESTVSTLYISPSNIETGQKIICRASNKA 185
BLAST of NPHS1-1 vs. Ensembl Cavefish
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:HGNC Symbol;Acc:HGNC:23204]) HSP 1 Score: 74.3294 bits (181), Expect = 7.449e-13 Identity = 55/194 (28.35%), Postives = 85/194 (43.81%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIND-----TANSLSMKSSQIMNGDIVTCGVENNA 850
V+ G+ V + C + G + W G LG R++ YPR + G+ G+H L I+ V++ D +ECQ AA R R +LTVL P P+I VS++ + +NLTC + N P + W +++G G S + D T ++L + S I G + C N A
Sbjct: 11 VVAGQPVTLPCSIPGYHGVVLWIKDGLALGVGRDLSGYPRYTVVGDHSSGEHHLRIQRVELMDDAVFECQAIQAAM---RSRPARLTVLVPPEDPVI-------DGGPIVSLRAGD---PLNLTCHADNAKPAASIIW------IRNGEVLNGAMYSKTLLRDGRRESTVSTLYISPSNIETGQKIICRASNKA 185
BLAST of NPHS1-1 vs. Ensembl Cavefish
Match: kirrel3l (kirre like nephrin family adhesion molecule 3, like [Source:ZFIN;Acc:ZDB-GENE-070831-1]) HSP 1 Score: 73.559 bits (179), Expect = 1.631e-12 Identity = 41/138 (29.71%), Postives = 67/138 (48.55%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEW 697
V+ G+ V + C + +G +QW G LG +R++P + R + G+ G+HSL IE+ +++D YECQ + A + R R LTVL PS P++ + + + + NLTC + P W
Sbjct: 27 VVAGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWTRYSLLGDPASGEHSLVIESSELADDAVYECQATQAGLRSHRAR---LTVLVPPSDPVVDGG----------PVVRLKAHTPHNLTCRATGAKPAAEITW 151
BLAST of NPHS1-1 vs. Ensembl Cavefish
Match: kirrel3l (kirre like nephrin family adhesion molecule 3, like [Source:ZFIN;Acc:ZDB-GENE-070831-1]) HSP 1 Score: 73.1738 bits (178), Expect = 1.741e-12 Identity = 41/138 (29.71%), Postives = 67/138 (48.55%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEW 697
V+ G+ V + C + +G +QW G LG +R++P + R + G+ G+HSL IE+ +++D YECQ + A + R R LTVL PS P++ + + + + NLTC + P W
Sbjct: 24 VVAGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWTRYSLLGDPASGEHSLVIESSELADDAVYECQATQAGLRSHRAR---LTVLVPPSDPVVDGG----------PVVRLKAHTPHNLTCRATGAKPAAEITW 148
BLAST of NPHS1-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002463.1 (pep scaffold:Pmarinus_7.0:GL476400:5068:120393:-1 gene:ENSPMAG00000002229.1 transcript:ENSPMAT00000002463.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 53.1434 bits (126), Expect = 7.173e-7 Identity = 58/214 (27.10%), Postives = 88/214 (41.12%), Query Frame = 2
Query: 3077 GKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRF-NGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPK--PIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEK 3709
G LF G P P V+W E DN + AQ E +L + + N D GKY N G+ S V + SP+ P+NF V +VT+ VSV W +GGSK + + T + A + F + +L Y +++VNE+ GIP P ++ Y P P + +++ +K + EK
Sbjct: 11027 GTLRLFVPIKGRPDPEVRW------EKEDN--VHLRDRAQIEVTSSYTMLVVDNVNRFDSGKYTLNLENSSGKKSAFVNVRILDSPSAPVNFKVKDVTKNYVSVTWDPPLIDGGSKITAYIVEKREST------RKAYVTVTNSCHKTSFKVEDLLEGCHYYFRVVAVNEF--GMGIPADTPEAVRAAEYPLP-PGKVTLRDVTKNSASISWEK 11223 HSP 2 Score: 49.6766 bits (117), Expect = 8.732e-6 Identity = 48/170 (28.24%), Postives = 78/170 (45.88%), Query Frame = 2
Query: 3101 FIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGW-ILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIP 3607
F G PVP +KW+ DN + + AQ E G ++I + + D GKY+ N G S V + +P P+N TV ++T+ S ++ W +GG++ IK Y + D + ANI K+ F ++NL + +++ NEY G+P
Sbjct: 9952 FRGRPVPDIKWL------KDDNEI---SDKAQIERGLNYTSISIDNCDRYDAGKYVLNLENSAGTKSAFVSVKVFDTPGPPLNLTVKDITKDSATLTWEPPAIDGGAR--IKNY--VIDKRESTRKAYANITTQCHKN--SFKVHNLLEGGIFYFRVMAENEY--GLGVP 10104
BLAST of NPHS1-1 vs. Ensembl Nematostella
Match: EDO45224 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RTJ3]) HSP 1 Score: 48.521 bits (114), Expect = 9.967e-6 Identity = 30/102 (29.41%), Postives = 45/102 (44.12%), Query Frame = 2
Query: 3038 KNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQP 3343
++ KS ++ + L CY G P P + W NP + TSV PG +VL ++ +E D G Y C A N G HV+ + + P +P
Sbjct: 19 RSLSKSNTSSWVEHTVSLHCYVSGSPPPVITWY---------NPASHVTSV----PG--GSVLTVTTRSEQDYGSYTCRAVNSVGSHEHVISVIQWTPPGKP 105
BLAST of NPHS1-1 vs. Ensembl Medaka
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:NCBI gene;Acc:101155460]) HSP 1 Score: 118.627 bits (296), Expect = 2.814e-26 Identity = 246/1127 (21.83%), Postives = 422/1127 (37.44%), Query Frame = 2
Query: 248 QCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPA-ASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFT-SDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKS--SNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSK----LIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANP-SREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGK--SELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPS 3595
Q FR P V G ++ C V G +QW G +LG R++P +PR M G G + L IEN ++ D YECQ + S+ L VL PS P + + + + +D +TCV+ + P +H F V I T S + + NG ++ C +N A K + M V+ P I + E V G L L CV + G+P L+W+ N + I + + S++ +M I+ ++ EL C P S T + V + P ++ + ++ EGQ+V LSC + + V I W++ E TVF +G +T+ V F +D + L+ K T F+ + KI ++ P + L+ ++ L G L C TGG+PTP L L + ++ R E ++ + P DN +RC + NN SA ++L+ A + MKI + + L + ++C +SS P A I W + G+ ++K +Q + +++ + LT + + C N VL + ++ V +PP+F + P ++Q VE+ +I + A+P WL ++ + K D S++ I NA Y++ EN G + L++ Y + A ++ + VN A ++C A ANP + + +W+ + E +++ + Q+ SS + I + G Y+C N ++ ++LV +P K + + A++ G +EL C G P W L NP +Y + + + ++ + D + C A N GE + L P P F ++ T SV++ WI FNGG +Q+ ++ ++ +V +Q ++F D FT+ L Y SI ++N S
Sbjct: 50 QAFRIEPR-NLTVRMGATAVLKCEVLRASGTVQWVKDGLLLGPQRSLPGFPRYSMIGKR--GQYHLRIENAQLEDDTFYECQAGQSDTSEGIISNAVWLDVLIPPSNPYFTMD------MAAYWVAGESYD----VTCVAPDAKP-----------EAEHFF------VCIFTFGSCVTSERVTALSSDNGRLLACHAKNAALSKPV-----VTTVTMNVYYP--PDAPFIVNLDRDE----VRAGRMLMLECV-SGSGNPLAALRWSKNGE---IVSTTWEADEFARKSRSVLKMV-ITPADNQAELSCESFNAVSLSPLSITRKLDVFFDPEELTLSG-------------SLEAIEGQEVVLSCFAAS-SNPPVDIRWWLGYKE----------LNNTVFIREEGDNGGMTTVSNVTYRFSRED------NGLMLNCEAFNKGTRFS--KIKDAKISVYYPPQ--KVWLDAPAQDVPLNAGTKVTLICHSTGGNPTPTLTWLKNGKAVAHAPKQTSSDMAAAR-ELHLVLTPSDNMATYRCDAT-NNAKKTISAHAKLLVYFTA-----VSMKITAHQEVLRQGEVLTLECLAESSNPTANIFW-----SVGAVRFGVTLSTK------------------HQSGAVTVNSTLLLNLTSQHHKQNVICQ--------AYNPVLEEGANTYYRLNVLHPPEFSSAQ--PTEIQ---VEEDEMATIPLLVSANPEGVSCVWLHRRETLVKG-DPDGSLE---IRNATRRDAGLYTIKCENEEGVSQTAITLDVLYACDVT-AEKDPVFVN----LGGTADLICVADANPIISDMFSWKWL--------EGERVEMGEETQEDESS------------------------------LLTIRNVTRAHSGLYQCTANNGIGLSGSVN-MQLVVQFKPELQKGPQWRKVASRGDGSTTAELVCRAEGIPRIDFSWEKKGVFLDLANP-RYEEKTVREGSFHTSTLRVVNVSAVMDYAVFSCYARNSLGEDKVDIQLVSTNHPDPPSMFRHVSSTHDSVTLEWIPGFNGGLQQKFRIRYQWDQSV---SFQYVDVF---PPDATTFTVTGLQPVTVYNFSINALNAIGES 998
BLAST of NPHS1-1 vs. Ensembl Medaka
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:NCBI gene;Acc:101155460]) HSP 1 Score: 110.153 bits (274), Expect = 1.029e-23 Identity = 250/1142 (21.89%), Postives = 417/1142 (36.51%), Query Frame = 2
Query: 248 QCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPA-ASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSI-----ETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFT-SDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKS--SNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTS-INVKLTPEFDLSFIECMIIPHEKWITINKVLSK----LIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKK---IHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANP-SREQLTVEWIREISKIL------DQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGK--SELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPS 3595
Q FR P V G ++ C V G +QW G +LG R++P +PR M G G + L IEN ++ D YECQ + S+ L VL PS P + + + + +D +TCV+ + P K G K G + S + + +T +++ + NG ++ C +N A K + M V+ P I + E V G L L CV + G+P L+W+ N + I + + S++ +M I+ ++ EL C P S T + V + P ++ + ++ EGQ+V LSC + + V I W++ E TVF +G +T+ V F +D + L+ K T F+ + KI ++ P + L+ ++ L G L C TGG+PTP L L + ++ R E ++ + P DN +RC + NN SA ++L+ A + MKI + + L + ++C +SS P A I W S+ Q + KS T S + + LT + + C N VL + ++ V +PP+F + P ++Q VE+ +I + A+P WL ++ + K H I NA Y++ EN G + L++ Y + A ++ + VN A ++C A ANP + + +W+ E + D+ LL I T H G Y+C N ++ ++LV +P K + + A++ G +EL C G P W L NP +Y + + + ++ + D + C A N GE + L P P F ++ T SV++ WI FNGG +Q+ ++ ++ +V +Q ++F D FT+ L Y SI ++N S
Sbjct: 27 QAFRIEPR-NLTVRMGATAVLKCEVLRASGTVQWVKDGLLLGPQRSLPGFPRYSMIGKR--GQYHLRIENAQLEDDTFYECQAGQSDTSEGIISNAVWLDVLIPPSNPYFTMD------MAAYWVAGESYD----VTCVAPDAKP------EAEVILYKDGVKLTGVESSSVSGSKDKLLNTLAEVTVTALSSDNGRLLACHAKNAALSKPV-----VTTVTMNVYYP--PDAPFIVNLDRDE----VRAGRMLMLECVSGS-GNPLAALRWSKNGE---IVSTTWEADEFARKSRSVLKMV-ITPADNQAELSCESFNAVSLSPLSITRKLDVFFDPEELTLSG-------------SLEAIEGQEVVLSCFAAS-SNPPVDIRWWLGYKE----------LNNTVFIREEGDNGGMTTVSNVTYRFSRED------NGLMLNCEAFNKGTRFS--KIKDAKISVYYPPQ--KVWLDAPAQDVPLNAGTKVTLICHSTGGNPTPTLTWLKNGKAVAHAPKQTSSDMAAAR-ELHLVLTPSDNMATYRCDAT-NNAKKTISAHAKLLVYFTA-----VSMKITAHQEVLRQGEVLTLECLAESSNPTANIFW----------------------SVGAVRFQKAEQTHSKDKSGAVTVNSTLLLNLTSQHHKQNVICQ--------AYNPVLEEGANTYYRLNVLHPPEFSSAQ--PTEIQ---VEEDEMATIPLLVSANPEGVSCVWLHRRETLVKGNNLHHYWLEDGSLEIRNATRRDAGLYTIKCENEEGVSQTAITLDVLYACDVT-AEKDPVFVN----LGGTADLICVADANPIISDMFSWKWLGEEEVEMGEETQEDESSLLTIRN------------VTRAHS--------------------------------GLYQCTANNGIGLSGSVN-MQLVVQFKPELQKGPQWRKVASRGDGSTTAELVCRAEGIPRIDFSWEKKGVFLDLANP-RYEEKTVREGSFHTSTLRVVNVSAVMDYAVFSCYARNSLGEDKVDIQLVSTNHPDPPSMFRHVSSTHDSVTLEWIPGFNGGLQQKFRIRYQWDQSV---SFQYVDVF---PPDATTFTVTGLQPVTVYNFSINALNAIGES 1000
BLAST of NPHS1-1 vs. Ensembl Medaka
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:NCBI gene;Acc:101170290]) HSP 1 Score: 78.1814 bits (191), Expect = 5.169e-14 Identity = 58/195 (29.74%), Postives = 86/195 (44.10%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETI------NDTANSLSMKSSQIMNGDIVTCGVENNA 850
V+ G+ V + C + G + W G LG R++ YPR + G+ G+H L I+ V++ D +ECQ AA R R +LTVL P P+I VS++ + +NLTC + N P + W N L +KVSIE + T ++L + S I G + C N A
Sbjct: 29 VVAGQPVTLPCSIPGYHGIVLWIKDGLALGVGRDLSGYPRYTVIGDHGSGEHHLRIQRVELMDDAVFECQAVQAAM---RSRPARLTVLVPPDDPVI-------GGGPVVSLRAGD---PLNLTCHADNAKPAASIIWMRNGEVLNGAMY---SKVSIEALVRDGRRESTVSTLYLSPSNIETGQQIICRASNKA 207
BLAST of NPHS1-1 vs. Ensembl Medaka
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:NCBI gene;Acc:101170290]) HSP 1 Score: 75.485 bits (184), Expect = 3.571e-13 Identity = 55/194 (28.35%), Postives = 85/194 (43.81%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIND-----TANSLSMKSSQIMNGDIVTCGVENNA 850
V+ G+ V + C + G + W G LG R++ YPR + G+ G+H L I+ V++ D +ECQ AA R R +LTVL P P+I VS++ + +NLTC + N P + W +++G G S + D T ++L + S I G + C N A
Sbjct: 57 VVAGQPVTLPCSIPGYHGIVLWIKDGLALGVGRDLSGYPRYTVIGDHGSGEHHLRIQRVELMDDAVFECQAVQAAM---RSRPARLTVLVPPDDPVI-------GGGPVVSLRAGD---PLNLTCHADNAKPAASIIW------MRNGEVLNGAMYSKALVRDGRRESTVSTLYLSPSNIETGQQIICRASNKA 231
BLAST of NPHS1-1 vs. Ensembl Medaka
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:NCBI gene;Acc:101170290]) HSP 1 Score: 75.485 bits (184), Expect = 3.845e-13 Identity = 55/194 (28.35%), Postives = 85/194 (43.81%), Query Frame = 2
Query: 284 VLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETIND-----TANSLSMKSSQIMNGDIVTCGVENNA 850
V+ G+ V + C + G + W G LG R++ YPR + G+ G+H L I+ V++ D +ECQ AA R R +LTVL P P+I VS++ + +NLTC + N P + W +++G G S + D T ++L + S I G + C N A
Sbjct: 99 VVAGQPVTLPCSIPGYHGIVLWIKDGLALGVGRDLSGYPRYTVIGDHGSGEHHLRIQRVELMDDAVFECQAVQAAM---RSRPARLTVLVPPDDPVI-------GGGPVVSLRAGD---PLNLTCHADNAKPAASIIW------MRNGEVLNGAMYSKALVRDGRRESTVSTLYLSPSNIETGQQIICRASNKA 273
BLAST of NPHS1-1 vs. Planmine SMEST
Match: SMESG000003359.1 (SMESG000003359.1) HSP 1 Score: 3348.53 bits (8681), Expect = 0.000e+0 Identity = 1614/1616 (99.88%), Postives = 1615/1616 (99.94%), Query Frame = 2
Query: 92 MICVFVLSNTFINRIEFVLCQDPFNVDDLDAAESISLSQSSTCTEKDDIHRIQCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSREKLSSPPTHQRMDTVGFISNDDSIEMRPPSLVRSGIESNLYAQSISDHMSQNGSDTGLKLNYYKTHSDDQFSSPFLGPELDLVASVRGRNNYDINALSPNFHPGYIQFAESSSMTSAQDPYIKGYYTQRPYYNSQTPSHGVIRKSSHSSNDTVTLPQYPISSSYQKRMDKVCFPQSAVSISTNSGDSNCSQSPPRKYIGYSPMVNQSGAPSTVEEYLSSGNSASESHRFGINGSDEYFTSPMSSFRLNQGLDSMSNKDFYSQLCSTNQQNQESAQEFAIQRIMYGNDVSAITPYRAYNDHQRLSTSSDNEHLLKPPLDFSGIESNNSNNIQSPMADAHKLSYVDLIAHSSPSVIRFTGPISENL 4939
MICVFVLSNTFINRIEFVLCQDPFNVDDLDAAESISLSQSSTCTEKDDIHRIQCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAIS+KTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSREKLSSPPTHQRMDTVGFISNDDSIEMRPPSLVRSGIESNLYAQSISDHMSQNGSDTGLKLNYYKTHSDDQFSSPFLGPELDLVASVRGRNNYDINALSPNFHPGYIQFAESSSMTSAQDPYIKGYYTQRPYYNSQTPSHGVIRKSSHSSNDTVTLPQYPISS YQKRMDKVCFPQSAVSISTNSGDSNCSQSPPRKYIGYSPMVNQSGAPSTVEEYLSSGNSASESHRFGINGSDEYFTSPMSSFRLNQGLDSMSNKDFYSQLCSTNQQNQESAQEFAIQRIMYGNDVSAITPYRAYNDHQRLSTSSDNEHLLKPPLDFSGIESNNSNNIQSPMADAHKLSYVDLIAHSSPSVIRFTGPISENL
Sbjct: 1 MICVFVLSNTFINRIEFVLCQDPFNVDDLDAAESISLSQSSTCTEKDDIHRIQCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISVKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSREKLSSPPTHQRMDTVGFISNDDSIEMRPPSLVRSGIESNLYAQSISDHMSQNGSDTGLKLNYYKTHSDDQFSSPFLGPELDLVASVRGRNNYDINALSPNFHPGYIQFAESSSMTSAQDPYIKGYYTQRPYYNSQTPSHGVIRKSSHSSNDTVTLPQYPISSGYQKRMDKVCFPQSAVSISTNSGDSNCSQSPPRKYIGYSPMVNQSGAPSTVEEYLSSGNSASESHRFGINGSDEYFTSPMSSFRLNQGLDSMSNKDFYSQLCSTNQQNQESAQEFAIQRIMYGNDVSAITPYRAYNDHQRLSTSSDNEHLLKPPLDFSGIESNNSNNIQSPMADAHKLSYVDLIAHSSPSVIRFTGPISENL 1616
BLAST of NPHS1-1 vs. Planmine SMEST
Match: SMESG000003359.1 (SMESG000003359.1) HSP 1 Score: 3172.87 bits (8225), Expect = 0.000e+0 Identity = 1529/1531 (99.87%), Postives = 1530/1531 (99.93%), Query Frame = 2
Query: 77 MLNFWMICVFVLSNTFINRIEFVLCQDPFNVDDLDAAESISLSQSSTCTEKDDIHRIQCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSREKLSSPPTHQRMDTVGFISNDDSIEMRPPSLVRSGIESNLYAQSISDHMSQNGSDTGLKLNYYKTHSDDQFSSPFLGPELDLVASVRGRNNYDINALSPNFHPGYIQFAESSSMTSAQDPYIKGYYTQRPYYNSQTPSHGVIRKSSHSSNDTVTLPQYPISSSYQKRMDKVCFPQSAVSISTNSGDSNCSQSPPRKYIGYSPMVNQSGAPSTVEEYLSSGNSASESHRFGINGSDEYFTSPMSSFRLNQGLDSMSNKDFYSQLCSTNQQNQESA 4669
MLNFWMICVFVLSNTFINRIEFVLCQDPFNVDDLDAAESISLSQSSTCTEKDDIHRIQCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAIS+KTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSREKLSSPPTHQRMDTVGFISNDDSIEMRPPSLVRSGIESNLYAQSISDHMSQNGSDTGLKLNYYKTHSDDQFSSPFLGPELDLVASVRGRNNYDINALSPNFHPGYIQFAESSSMTSAQDPYIKGYYTQRPYYNSQTPSHGVIRKSSHSSNDTVTLPQYPISS YQKRMDKVCFPQSAVSISTNSGDSNCSQSPPRKYIGYSPMVNQSGAPSTVEEYLSSGNSASESHRFGINGSDEYFTSPMSSFRLNQGLDSMSNKDFYSQLCSTNQQNQESA
Sbjct: 1 MLNFWMICVFVLSNTFINRIEFVLCQDPFNVDDLDAAESISLSQSSTCTEKDDIHRIQCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISVKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSREKLSSPPTHQRMDTVGFISNDDSIEMRPPSLVRSGIESNLYAQSISDHMSQNGSDTGLKLNYYKTHSDDQFSSPFLGPELDLVASVRGRNNYDINALSPNFHPGYIQFAESSSMTSAQDPYIKGYYTQRPYYNSQTPSHGVIRKSSHSSNDTVTLPQYPISSGYQKRMDKVCFPQSAVSISTNSGDSNCSQSPPRKYIGYSPMVNQSGAPSTVEEYLSSGNSASESHRFGINGSDEYFTSPMSSFRLNQGLDSMSNKDFYSQLCSTNQQNQESA 1531
BLAST of NPHS1-1 vs. Planmine SMEST
Match: SMESG000003359.1 (SMESG000003359.1) HSP 1 Score: 3162.47 bits (8198), Expect = 0.000e+0 Identity = 1524/1526 (99.87%), Postives = 1525/1526 (99.93%), Query Frame = 2
Query: 92 MICVFVLSNTFINRIEFVLCQDPFNVDDLDAAESISLSQSSTCTEKDDIHRIQCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSREKLSSPPTHQRMDTVGFISNDDSIEMRPPSLVRSGIESNLYAQSISDHMSQNGSDTGLKLNYYKTHSDDQFSSPFLGPELDLVASVRGRNNYDINALSPNFHPGYIQFAESSSMTSAQDPYIKGYYTQRPYYNSQTPSHGVIRKSSHSSNDTVTLPQYPISSSYQKRMDKVCFPQSAVSISTNSGDSNCSQSPPRKYIGYSPMVNQSGAPSTVEEYLSSGNSASESHRFGINGSDEYFTSPMSSFRLNQGLDSMSNKDFYSQLCSTNQQNQESA 4669
MICVFVLSNTFINRIEFVLCQDPFNVDDLDAAESISLSQSSTCTEKDDIHRIQCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAIS+KTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSREKLSSPPTHQRMDTVGFISNDDSIEMRPPSLVRSGIESNLYAQSISDHMSQNGSDTGLKLNYYKTHSDDQFSSPFLGPELDLVASVRGRNNYDINALSPNFHPGYIQFAESSSMTSAQDPYIKGYYTQRPYYNSQTPSHGVIRKSSHSSNDTVTLPQYPISS YQKRMDKVCFPQSAVSISTNSGDSNCSQSPPRKYIGYSPMVNQSGAPSTVEEYLSSGNSASESHRFGINGSDEYFTSPMSSFRLNQGLDSMSNKDFYSQLCSTNQQNQESA
Sbjct: 1 MICVFVLSNTFINRIEFVLCQDPFNVDDLDAAESISLSQSSTCTEKDDIHRIQCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQNISVSIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNINEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISVKTNVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFDLSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSREKLSSPPTHQRMDTVGFISNDDSIEMRPPSLVRSGIESNLYAQSISDHMSQNGSDTGLKLNYYKTHSDDQFSSPFLGPELDLVASVRGRNNYDINALSPNFHPGYIQFAESSSMTSAQDPYIKGYYTQRPYYNSQTPSHGVIRKSSHSSNDTVTLPQYPISSGYQKRMDKVCFPQSAVSISTNSGDSNCSQSPPRKYIGYSPMVNQSGAPSTVEEYLSSGNSASESHRFGINGSDEYFTSPMSSFRLNQGLDSMSNKDFYSQLCSTNQQNQESA 1526
BLAST of NPHS1-1 vs. Planmine SMEST
Match: SMESG000021277.1 (SMESG000021277.1) HSP 1 Score: 426.402 bits (1095), Expect = 1.667e-123 Identity = 350/1269 (27.58%), Postives = 605/1269 (47.68%), Query Frame = 2
Query: 188 ESISLSQSSTCTEKDDIHRIQCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPA-ASQPPRRRMTKLTVLKVPSTPIIYQNGQSNKQN---ISVSIKK-DEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANS-LSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWN--------INEDDLMIAQDKILSVKSEHFSQNTFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPSKIEMEAISIKTNVSLHNPST--------VYCKEGQKVKLSCTSDNFFTDNVRILWFIKSSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQ--------LKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDR---EMKSSNLHEIGIT-----RLEYNIEVKPRDNEKKFRCIVSYNNVMDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEM-QCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTF--TSINVKLTPEFDLSFIECMIIPHEKW-ITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQ-TFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKKIHIDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLGASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDIL-KGQQKFISSD-PIRCTNTHQWNKGKVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKS-ELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEPVESNNFLIIILLATVGV-LLLTIKIGLIVYFVRKRKLK-HRKSRE 3850
E++ QS C E++ + +QCF+ELP Y+ L G+ +++ C VA+Q+GK QW + +MLGF+RNIP PR MTG+ GD SL IENV +SD+G YECQ++ + RR+T LTVL PS P+IY+N + I++S K D + +NL C S G P PTFEW N LK ++ ++ I+ +S L++ +Q+MNGD +TC V N AT + ++ +KT + + LPG +++DI+ + ++EG L LSC G+PP +KW+ ++++ +++D I + + + I R +H + C + D + V I V Y P I +++ K PS VY + QK+ CT+ ++ V + W+ E D +F K + + + VDK + +S+ V KS E++ Q + +E + PS+P+L ++ + +T+ + C GG+PTP L L + E S+ + I +T R N++V +RC ++ + F++ S LQV PP +L + I + N + + F + C TD S P +I W + C++ + K ++ C +++ + K S ++I + LT + + + +EC I + I+I K L + V++PP F D+Q +VV +G +I + ++PS+ Y WL P+ + + +I+ + +MM NY+L+ N +G +F F LN+T+G L+G E I V P + +A ++C AQ+NP+ Q V W ++LD+ +L K +Q+ ISS I+C + K +CIS ++EI++T+ I + +G Y+C++ N+ + A K + L P V + R+ +F+ G+S +LFC+ G+P P V W N +L + K+ + G + ++L ++ + D Y C A N G ++ + L+ P P N V+N+T ++V W FNGG +Q+ + W+ + + D + + E+ + + I +L + +YEI I S ++ S PI++KT + SI+ + K +K ++SNN +I I +V V ++L I I + +++RKR+ K H+ S +
Sbjct: 52 EAVLFHQSELCQEEELKNNLQCFKELPEENYEALIGETIIIKCIVANQKGKAQWRIEDRMLGFNRNIPFKPRFSMTGDEEAGDQSLRIENVTISDAGEYECQVTRGNYGKKELRRLTHLTVLVPPSQPLIYENNNRQPSSTILITISAKMYDNHNKKLNLKCESKEGQPDPTFEWYHNSQMLKE------QELLVKIIDYKTHSFLTISFTQLMNGDSITCAVFNKATLRNSQTSQTKLKTSVSIAINTLPGA---PKITDIKNKIIISEGKSLSLSCQSQPAGEPPGSIKWDWADGNKKQLDQNRYALSKDWINTFVDSGILLSRITIPKIDRSYHGLFILCKLNHD-LLDDQTDQVQIWVEYGPDNITIDSSVGKPKRITKYPSNDIGMDIPIVYAQPNQKLLFQCTTSAYY-GKVHLSWY----ESED-----------IFNHKDNWKLL-DQVTTNENMVDKGLRKVQISQ-FETVHSAEKSVLMKCELKFQDSYKRSSLVMVETIKMPSIPILKMDK----EYITDKSEVTISCQTYGGNPTPSLTLYKGHQKIKTEFGKSDPNRITLTHKFIARKSMNLDV--------YRCGIT-SPFRLFDNLKSNETKLQVFYPPTKLSLTI--APGNSMRSGTFNVFHCRTDESNPAPEIT-WTKYDCENKNFNYQIDREKLLK--CAYKVIQSTYTVTIGKWNGSFVLQSTIKIPLTEDDNKNILECSINSMAGFKISIRKALV----LTVKHPPIF------KKDIQYDYVVTEGQPITIYLNPASNPSVQSYQWLSEGSPIPYSRALRVQDNSLTIIRSDVDMMRNYTLIARNEIGTGSFTFRLNVTFGARLIGPDSEDISVKPDEK---EAKLICKAQSNPTGSQTRVIW-----QMLDKTYNWRLLYKERQEVISSSGSIQCNGDYWTYLAKYRASCISVSNIEIMSTLHIWNLVPSDMGRYQCLVNNTIES-AAGKVLNLNVPVGATIVIS-RRRQFSGGELGQSAKLFCWVFGYPKPKVIWYRDISNLTLISGKKFQYDSERLGSGLYNSILTVNFIEKQDYMNYFCQASNDIGSATARIRLSSSTKPDIPFNIRVVNITNKMIAVEWEQGFNGGFEQEFIVSWK-SVSRFSSDIHSVQLVENMLDEKLIYVIKDLLSSTQYEIRICSKSKIFQSCQFSSPILVKTKKTSSSEESSIYSTSSFKTTKDK--------LKSNNIIITITSVSVFVFVILVINILFVAFYLRKRRNKSHQNSNQ 1245
BLAST of NPHS1-1 vs. Planmine SMEST
Match: SMESG000028689.1 (SMESG000028689.1) HSP 1 Score: 392.119 bits (1006), Expect = 1.327e-111 Identity = 368/1455 (25.29%), Postives = 658/1455 (45.22%), Query Frame = 2
Query: 170 DDLDAAESISLSQSSTCTEKDDIHRIQCFRELPSPKYDVLEGKDVLMGCRVAHQQGKLQWAFKGKMLGFDRNIPTYPRMRMTGNAVIGDHSLFIENVKVSDSGNYECQLSPAASQPP-RRRMTKLTVLKVPSTP-IIYQNGQSNKQNISV-SIKKDEFDSNINLTCVSLNGNPVPTFEWSVNRSPLKHGFKFRGNKVSIETINDTANSLSMKSSQIMNGDIVTCGVENNATRKMFNLTERIIKTQMRVFKRILPGKIVIKEVSDIEKTVYVTEGHQLQLSCVCNNPGDPPIDLKWNI----------NEDDLMIAQDKILSVKSEHFSQN----TFRMSNISRKWHRKELYCSGSQDNYAIPSSATVLILVNYPPS---KIEMEAISIKT-NVSLHNPSTVYCKEGQKVKLSCTSDNFFTDNVRILWFIK----------SSEESDWKPITTSFEKTVFTSDGRLKYITSSLEVPPSFVDKDISSHILSRLLSQVVCIVKSTEFNIEIRRQLKIELFEEPSVPVLTLNNLSKPQILTNGESAKLKCTVTGGHPTPQLRLLDERDREMKSSNLHEIGITRLEYNIEVKPRDNEKKFRCIVSYNNV-MDFESASSELMALQVANPPKQLHMKIISSMDNLIANQNFEMQCHTDSSFPEAKIEWWWHHSCKSSHTLGIRSTSKDVRSLCQSEILNEPQNINYQKSTQSTFTSINVKLTPEFD--LSFIECMIIPHEKWITINKVLSKLIKIRVRYPPQFLNVHKLPNDVQTFVVEKGNNFSIAIKSIAHPSINGYTWLVNKQPMEKK-IH-IDTSVDKFSIVNAQPEMMANYSLLVENNLGKAAFDFFLNITYGPTLLG---ASQETIKVNPQMEHNHKAMIMCSAQANPSREQLTVEWIREISKILDQEELLDILKGQQKFISSDPIRCTNTHQWNKG-------KVNVNCISFGDVEIVTTMQIMPIDYRFIGSYRCIIKNSDKRPPAIKTIKLVYPTRPNPVKNFRKSEFAAQIFGKSELFCYFIGFPVPTVKWMTFYKNESLDNPVKYFTSVAQTEPGYFKAVLAISDTNESDLGKYICVAGNQYGESSHVVILNKPRSPTQPINFTVINVTETSVSVGWILRFNGGSKQQIKLYWRLRDTVVNGDYQNANIFEDEEKDVQFFTIYNLYRNREYEISIISVNEYSPSAGIPKPIIIKTMNYVDPDPDSIHIQNDSKRGFNKQLEKRIEP--VESNNFLIIILLATVGVLLLTIKIGLIVYFVRKRKLKHRKSR--EKLSSPPTHQRMDTVGFISNDDSIEMRPPSLVRSGIESNLYAQSISDHMSQNGSDTGLKLNYYKTHS------DDQ--FSSPFLGPELDLVASVRGRNNYDINALSPNFHPGYIQFAESSSMTSAQDPYIKGYYTQRPYYNSQTP---SHGVIRKSSHSSNDTVTLPQYPISSSYQKRM 4351
DD + + + QS CT ++ ++RIQCF+E+P P Y+V +G + C V +Q GK+QW +LG+DRNIP P R+ G+ V G+H L I NV V D+G YECQ+ P + P RR TKL VL P P I Y+ Q N + + SIK+ D TCVS NG P F+W +N + ++ N +E +N + + LS+K+S+ +GD++ C V N AT + R + T++ + PG+ I +++ + T YV + + + ++C + G P L W N + +D + S+N T + ++ K H +L+C + + S+A L +++ P + ++S +T N + +Y + G++ ++CT+D ++ + I W S E WK E F + +K I + + SF+D S I S ++ ++K+ + P P + N+ KP L + + ++ C+ GG+P P+L+ + + ++SS + + + I + P N+ K + ++ + +S+ + L V PP + +K +S + + + ++QC S P A + W + + LG R V++ C E+L+ + + ++ P + L +++C + + + ++VL K I+I V + PQF + F +E+G + ++ ++ IA+P I YTW N + +K +H I TS K I NA+P + NY+L+V N+ G++ +LNITY P ++G +E I+ N ++E CS Q+NP+ ++ +W +K++ + DI + + I C N +W KG K C+ E+ + + I +D +G YRC++ N R +KTI+L P + + S+ A + + LFCY P P + W N S+ KY ++ + G++ A L I + +D G Y+C A N G S + ++ +P P+ V+N +++ + + WI F+GGS Q ++ W+ + Y+ ++ D+ K F I L + Y ISI S N ++ K I+ TM+ D+++ N+ I+P ++ N L I+ + G ++L + I +I++ +R+R+ + R ++ + ++P + +S DDS++ + +RS + LY + + S L N ++ HS DDQ FSS G + +R + + + PN G S+ A DP Y S TP HG+ + DT++ PI Q+++
Sbjct: 33 DDSEMSSGAKIKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIINSIKQ---DVRTEFTCVSDNGQPKVKFQWFLNNNLIQDN----ENSALLEELNYSRSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDN-IIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNE-ATIKWVFSPNPAINNDFISPVEKIWK------EDESFRRNSTIK-IKINKQFDQSFLDCQAWSGIKS------------------VQSRVKLHITYSPEKPTIQGYNVGKPVKLNH--NFEMTCSTIGGNPYPELQWFRD-SKPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSLSGAE-VENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRP----VQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVF-NPDYGEEHQVLRKRIQINVTFSPQFEGIINY-----NFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELE--------CSVQSNPANPHMSYKW----TKLVPGKGYNDI--SSSDLVPAFTIGC-NQAEW-KGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRCVVNNG-IRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKEKSPT--SLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRNSKHGTSQFSKTIMTNTMSA------------DNEKENNQPTYPIIQPNKLQDNTILFIVAASVFGFVILVVNILVIIFVLRRRRQRSRTAKLEARYNNPKSLLAASNGLILSRDDSLKDSNFNYLRSDM-VELYPRGLD-------SSQILHHNPHQIHSLRRSIPDDQKTFSSFETGSDDAREDLLRRQKEFGCCGIDPNSQKG--------SIIVAYDPITNSSYYAPANATSPTPIYLQHGMYDQ------DTMSTLLSPIHEGGQRQL 1386 The following BLAST results are available for this feature:
BLAST of NPHS1-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of NPHS1-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of NPHS1-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of NPHS1-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of NPHS1-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of NPHS1-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of NPHS1-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of NPHS1-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of NPHS1-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of NPHS1-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of NPHS1-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of NPHS1-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of NPHS1-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of NPHS1-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Cross References
External references for this transcript
Sequences
The following sequences are available for this feature:
transcript sequence >SMED30027960 ID=SMED30027960|Name=NPHS1-1|organism=Schmidtea mediterranea sexual|type=transcript|length=5087bpback to top protein sequence of SMED30027960-orf-1 >SMED30027960-orf-1 ID=SMED30027960-orf-1|Name=SMED30027960-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1622bp MLNFWMICVFVLSNTFINRIEFVLCQDPFNVDDLDAAESISLSQSSTCTEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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