U3 small nucleolar RNA associated 22

Overview
NameU3 small nucleolar RNA associated 22
Smed IDSMED30027947
Length (bp)1874
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of U3 small nucleolar RNA associated 22 (SMED30027947) t-SNE clustered cells

Violin plots show distribution of expression levels for U3 small nucleolar RNA associated 22 (SMED30027947) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of U3 small nucleolar RNA associated 22 (SMED30027947) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for U3 small nucleolar RNA associated 22 (SMED30027947) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30027947

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
muscle cellSMED30027947SMESG000015428.1 dd_Smed_v4_15147_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of U3 small nucleolar RNA associated 22 vs. TrEMBL
Match: A0A0X3NM38 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=TR153565 PE=4 SV=1)

HSP 1 Score: 184.111 bits (466), Expect = 2.674e-48
Identity = 98/344 (28.49%), Postives = 176/344 (51.16%), Query Frame = 2 
Query:  167 MGRGASRSKSGGMTVMKDSERKILLTNTNLSLFEFIQEGCFEKDLMAYCSNRLNDYSCIHWLDPETXXXXXXXXXXXXXLFTEDSKILCYHDREDQSMY-----------------------------------LMKLT-----QIVIPIILLIAFATSILSIFAIRKITKKLHPIATYIIYLALFDIVELIIQGGDVIIEAYFRIKVLAAIQILSRSSCQMLPFAYNLFKHSNVILYMAMAIDNWKFVKHPKQMNDAFKSEWIRNVVIVIAAVLMTLNAQFFWTFDVTENDSINLHNRTLKRTFQCSFSTTWPLKQVFTECIWPILDHLVGEVLPSLVCICCG 1078
            MGRG +R K+       D+E   +  N N+S+ EF+  G FE+D ++YC+NRL+DYSC++W DP  S         ++      S    +HD++  S+                                    L+KL       +++P + + A    +  + A+RK++    PIA++++Y+ + + + LI+Q  D ++  +  I+VL   Q + RS+CQ L  A + F+H   +LY+ +A+D  ++ K P+    +F      N++I+  A++ T+++QFFWTFD++  ++      +    +QC F ++WPL  +F    WP+ DHL+GE+LP ++ I  G
Sbjct:    1 MGRGGARGKASSAGQPYDTEAVFIPVNLNVSVVEFLLTGHFEQDYLSYCANRLHDYSCVYWFDPTVSHDLKVDALGSN--IESASATWDFHDQKQLSLTSKVLQNLHGSFSFFDGVGHQGWNQNTSKTILLVNDLIKLQTPGLWAVILPFLYISAILLYLPILHAMRKLSCTKLPIASFLVYILILEFISLILQLADCLLILFKGIRVLTIAQFVGRSACQSLSMANSCFRHVQSLLYLGLALDTLRYAKSPRYHYTSFNRNCAYNILILAIALMGTMDSQFFWTFDLSGVENHIASGDSADVLYQCDFLSSWPLNPIFVSYFWPLADHLIGEILPCVIPILAG 342
[Close]
BLAST of U3 small nucleolar RNA associated 22 vs. TrEMBL
Match: A0A183SM83 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS5331 PE=4 SV=1)

HSP 1 Score: 183.341 bits (464), Expect = 9.095e-48
Identity = 99/342 (28.95%), Postives = 181/342 (52.92%), Query Frame = 2 
Query:  167 MGRGASRSKSGGMTVMKDSERKILLTNTNLSLFEFIQEGCFEKDLMAYCSNRLNDYSCIHWLDP------ETXXXXXXXXXXXXXLFTEDSKILCYHDREDQSMY---------------------------LMKLT-----QIVIPIILLIAFATSILSIFAIRKITKKLHPIATYIIYLALFDIVELIIQGGDVIIEAYFRIKVLAAIQILSRSSCQMLPFAYNLFKHSNVILYMAMAIDNWKFVKHPKQMNDAFKSEWIRNVVIVIAAVLMTLNAQFFWTFDVTENDSINLHNRTLKRTFQCSFSTTWPLKQVFTECIWPILDHLVGEVLPSLVCICCG 1078
            MGRG +R K+       D+E   +  N N+S+ EF+  G FE+D ++YC+NRL+DYSC++W DP      +  +  S   S++++    D K L    +  Q+++                           L+KL       +++P + + A    +  + A+RK++    PIA++++Y+ + + + LI+Q  D ++  +  I+VL   Q + RS+CQ L  A + F+H   +LY+ +A+D  ++ K P+    +F      N++I+  A++ T+++QFFWTFD++  ++      +    +QC F ++WPL  +F    WP+ DHL+GE+LP ++ I  G
Sbjct:    1 MGRGGARGKASSAGQPYDTEAVFIPVNLNVSVVEFLLTGHFEQDYLSYCANRLHDYSCVYWFDPTVSHDLKVDALGSNIESASATWDLHDQKQLSLTSKVLQNLHGSFSFFDGVGHQGWNQNTSKTILLVNDLIKLQTPGLWAVILPFLYISAILLYLPILHAMRKLSCTKLPIASFLVYILILEFISLILQLADCLLILFKGIRVLTIAQFVGRSACQSLSMANSCFRHVQSLLYLGLALDTLRYAKSPRYHYTSFNRNCAYNILILAIALMGTMDSQFFWTFDLSGVENHIASGDSADVLYQCDFLSSWPLNPIFVSYFWPLADHLIGEILPCVIPILAG 342
[Close]
BLAST of U3 small nucleolar RNA associated 22 vs. TrEMBL
Match: A0A0X3NYT3 (Uncharacterized protein (Fragment) OS=Schistocephalus solidus OX=70667 GN=TR153565 PE=4 SV=1)

HSP 1 Score: 184.496 bits (467), Expect = 2.639e-47
Identity = 98/344 (28.49%), Postives = 176/344 (51.16%), Query Frame = 2 
Query:  167 MGRGASRSKSGGMTVMKDSERKILLTNTNLSLFEFIQEGCFEKDLMAYCSNRLNDYSCIHWLDPETXXXXXXXXXXXXXLFTEDSKILCYHDREDQSMY-----------------------------------LMKLT-----QIVIPIILLIAFATSILSIFAIRKITKKLHPIATYIIYLALFDIVELIIQGGDVIIEAYFRIKVLAAIQILSRSSCQMLPFAYNLFKHSNVILYMAMAIDNWKFVKHPKQMNDAFKSEWIRNVVIVIAAVLMTLNAQFFWTFDVTENDSINLHNRTLKRTFQCSFSTTWPLKQVFTECIWPILDHLVGEVLPSLVCICCG 1078
            MGRG +R K+       D+E   +  N N+S+ EF+  G FE+D ++YC+NRL+DYSC++W DP  S         ++      S    +HD++  S+                                    L+KL       +++P + + A    +  + A+RK++    PIA++++Y+ + + + LI+Q  D ++  +  I+VL   Q + RS+CQ L  A + F+H   +LY+ +A+D  ++ K P+    +F      N++I+  A++ T+++QFFWTFD++  ++      +    +QC F ++WPL  +F    WP+ DHL+GE+LP ++ I  G
Sbjct:    1 MGRGGARGKASSAGQPYDTEAVFIPVNLNVSVVEFLLTGHFEQDYLSYCANRLHDYSCVYWFDPTVSHDLKVDALGSN--IESASATWDFHDQKQLSLTSKVLQNLHGSFSFFDGVGHQGWNQNTSKTILLVNDLIKLQTPGLWAVILPFLYISAILLYLPILHAMRKLSCTKLPIASFLVYILILEFISLILQLADCLLILFKGIRVLTIAQFVGRSACQSLSMANSCFRHVQSLLYLGLALDTLRYAKSPRYHYTSFNRNCAYNILILAIALMGTMDSQFFWTFDLSGVENHIASGDSADVLYQCDFLSSWPLNPIFVSYFWPLADHLIGEILPCVIPILAG 342
[Close]
BLAST of U3 small nucleolar RNA associated 22 vs. TrEMBL
Match: A0A3R7H7G0 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CSKR_9161s PE=4 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 1.781e-39
Identity = 93/285 (32.63%), Postives = 154/285 (54.04%), Query Frame = 2 
Query:  446 EDQSMYLMKLTQIVIPIILLIAFATSILSIFAIRKITKKLHPIATYIIYLALFDIVELIIQGGDVIIEAYFRIKVLAAIQILSRSSCQMLPFAYNLFKHSNVILYMAMAIDNWKFVKHPKQMNDAFKSEWIRNVVIVIAAVLMTLNAQFFWTFDVTENDSINLHNRTLKRTFQCSFSTTWPLKQVFTECIWPILDHLVGEVLPSLVCICCGALYFQSKRVTDKNLSKYI-----------------------------INLDLVYQMNQALSIISLLFGIFSLPR 1213
            E   + L    +++IP +LL+A  ++I  +    K++ +  PIA Y +YL++FD ++L+ +G D ++EAY  I++  A+Q +SRSSCQ+L  A++  +H    L + ++ID  ++   P +    F+SEW +NV ++ A + + L++QF WTFD++ +D   LH R ++  +QC +S T  L  +F   +WPI+DHL G+VLP L C   G        V  +NL K                               INLDL+ +  +A +++ L  GIF LPR
Sbjct:  151 EYHRLPLNTAAKVIIPFLLLLACISNIFILRVSYKLSFRKLPIAIYFVYLSIFDQLDLLARGTDYLVEAYGGIRLFTAMQFVSRSSCQLLSMAHSAVRHLQSALLIGLSIDLCQWSAKPVRFWIRFRSEWTQNVALLAAILAIILDSQFLWTFDLSPSDITQLHGRPVQNIYQCGYSQTSTLSPLFLYYVWPIMDHLWGDVLPCLACTIAGIFSLICSDVRRRNLKKAKAVTQYWASRIQQNDRDQMSQLRCSVATRWINLDLIVETIRAFAMVLLTTGIFILPR 435

HSP 2 Score: 71.2478 bits (173), Expect = 4.406e-9
Identity = 30/64 (46.88%), Postives = 41/64 (64.06%), Query Frame = 2 
Query:  167 MGRGASRSKSGGMTVMKDSERKILLTNTNLSLFEFIQEGCFEKDLMAYCSNRLNDYSCIHWLDP 358
            MGRG  R+++ G T  +D          N++L +F+  G FEKDL +YC+NRL DYSC +WLDP
Sbjct:    1 MGRGGGRTRASGGTASQDGILTFQHLALNMTLAQFLISGYFEKDLFSYCANRLMDYSCTYWLDP 64
[Close]
BLAST of U3 small nucleolar RNA associated 22 vs. TrEMBL
Match: G7YLX8 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CLF_111729 PE=4 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 1.802e-39
Identity = 93/285 (32.63%), Postives = 154/285 (54.04%), Query Frame = 2 
Query:  446 EDQSMYLMKLTQIVIPIILLIAFATSILSIFAIRKITKKLHPIATYIIYLALFDIVELIIQGGDVIIEAYFRIKVLAAIQILSRSSCQMLPFAYNLFKHSNVILYMAMAIDNWKFVKHPKQMNDAFKSEWIRNVVIVIAAVLMTLNAQFFWTFDVTENDSINLHNRTLKRTFQCSFSTTWPLKQVFTECIWPILDHLVGEVLPSLVCICCGALYFQSKRVTDKNLSKYI-----------------------------INLDLVYQMNQALSIISLLFGIFSLPR 1213
            E   + L    +++IP +LL+A  ++I  +    K++ +  PIA Y +YL++FD ++L+ +G D ++EAY  I++  A+Q +SRSSCQ+L  A++  +H    L + ++ID  ++   P +    F+SEW +NV ++ A + + L++QF WTFD++ +D   LH R ++  +QC +S T  L  +F   +WPI+DHL G+VLP L C   G        V  +NL K                               INLDL+ +  +A +++ L  GIF LPR
Sbjct:   95 EYHRLPLNTAAKVIIPFLLLLACISNIFILRVSYKLSFRKLPIAIYFVYLSIFDQLDLLARGTDYLVEAYGGIRLFTAMQFVSRSSCQLLSMAHSAVRHLQSALLIGLSIDLCQWSAKPVRFWIRFRSEWTQNVALLAAILAIILDSQFLWTFDLSPSDITQLHGRPVQNIYQCGYSQTSTLSPLFLYYVWPIMDHLWGDVLPCLACTIAGIFSLICSDVRRRNLKKAKAVTQYWASRIQQNDRDQMSQLRCSVATRWINLDLIVETIRAFAMVLLTTGIFILPR 379
[Close]
BLAST of U3 small nucleolar RNA associated 22 vs. Planmine SMEST
Match: SMESG000015428.1 (SMESG000015428.1)

HSP 1 Score: 881.708 bits (2277), Expect = 0.000e+0
Identity = 484/485 (99.79%), Postives = 484/485 (99.79%), Query Frame = 2 
Query:  167 MGRGASRSKSGGMTVMKDSERKILLTNTNLSLFEFIQEGCFEKDLMAYCSNRLNDYSCIHWLDPETXXXXXXXXXXXXXLFTEDSKILCYHDREDQSMYLMKLTQIVIPIILLIAFATSILSIFAIRKITKKLHPIATYIIYLALFDIVELIIQGGDVIIEAYFRIKVLAAIQILSRSSCQMLPFAYNLFKHSNVILYMAMAIDNWKFVKHPKQMNDAFKSEWIRNVVIVIAAVLMTLNAQFFWTFDVTENDSINLHNRTLKRTFQCSFSTTWPLKQVFTECIWPILDHLVGEVLPSLVCICCGALYFQSKRVTDKNLSKYIINLDLVYQMNQALSIISLLFGIFSLPRVXXXXXXXXXXXXXXXXXXXXRESMKFKFNMNFSRNLNSSNEEVYLQYIELFVKYTDFLFMIVKFLILIKLVGLFRVKFVQMFTFNSKGKSNFVPRXXXXXXXXXXXXXLPTIKFVHNSVERKIPSENLTLCSYTM 1621
            MGRGASRSKSGGMTVMKDSERKILLTNTNLSLFEFIQEGCFEKDLMAYCSNRLNDYSCIHWLDPETSSSDSPSISSNSSLFTEDSKI CYHDREDQSMYLMKLTQIVIPIILLIAFATSILSIFAIRKITKKLHPIATYIIYLALFDIVELIIQGGDVIIEAYFRIKVLAAIQILSRSSCQMLPFAYNLFKHSNVILYMAMAIDNWKFVKHPKQMNDAFKSEWIRNVVIVIAAVLMTLNAQFFWTFDVTENDSINLHNRTLKRTFQCSFSTTWPLKQVFTECIWPILDHLVGEVLPSLVCICCGALYFQSKRVTDKNLSKYIINLDLVYQMNQALSIISLLFGIFSLPRVFYFSFKYFYYSYLKFHKKSFRESMKFKFNMNFSRNLNSSNEEVYLQYIELFVKYTDFLFMIVKFLILIKLVGLFRVKFVQMFTFNSKGKSNFVPRKCDKCSTKDCDCSLPTIKFVHNSVERKIPSENLTLCSYTM
Sbjct:    1 MGRGASRSKSGGMTVMKDSERKILLTNTNLSLFEFIQEGCFEKDLMAYCSNRLNDYSCIHWLDPETSSSDSPSISSNSSLFTEDSKIFCYHDREDQSMYLMKLTQIVIPIILLIAFATSILSIFAIRKITKKLHPIATYIIYLALFDIVELIIQGGDVIIEAYFRIKVLAAIQILSRSSCQMLPFAYNLFKHSNVILYMAMAIDNWKFVKHPKQMNDAFKSEWIRNVVIVIAAVLMTLNAQFFWTFDVTENDSINLHNRTLKRTFQCSFSTTWPLKQVFTECIWPILDHLVGEVLPSLVCICCGALYFQSKRVTDKNLSKYIINLDLVYQMNQALSIISLLFGIFSLPRVFYFSFKYFYYSYLKFHKKSFRESMKFKFNMNFSRNLNSSNEEVYLQYIELFVKYTDFLFMIVKFLILIKLVGLFRVKFVQMFTFNSKGKSNFVPRKCDKCSTKDCDCSLPTIKFVHNSVERKIPSENLTLCSYTM 485
[Close]
BLAST of U3 small nucleolar RNA associated 22 vs. Planmine SMEST
Match: SMESG000079147.1 (SMESG000079147.1)

HSP 1 Score: 296.204 bits (757), Expect = 1.500e-93
Identity = 166/434 (38.25%), Postives = 261/434 (60.14%), Query Frame = 2 
Query:  167 MGRGASRSKSGGMTVMKDSERKILLTNTNLSLFEFIQEGCFEKDLMAYCSNRLNDYSCIHWLDPETXXXXXXXXXXXXXLFTEDSKILCYHDREDQSMYLMKLTQIVIPIILLIAFATSILSIFAIRKITKKLHPIATYIIYLALFDIVELIIQGGDVIIEAYFRIKVLAAIQILSRSSCQMLPFAYNLFKHSNVILYMAMAIDNWKFVKHPKQMNDAFKSEWIRNVVIVIAAVLMTLNAQFFWTFDVTENDSINLHNRTLKRTFQCSFSTTWPLKQVFTECIWPILDHLVGEVLPSLVCI---CCGALYFQSKRVTDKNLSKYIINLDLVYQMNQALSIISLLFGIFSLPRVXXXXXXXXXXXXXXXXXXXXRESMKFKFNMNFSRNLNSS---NEEVYLQYIELFVKYTDFLFMIVKFLILIKLVGLFRVKF 1450
            MGRGA  SK GG+   KD   K+ +   N+S+ +F Q GCFEKDLM+YC+NRL++YSCI+WLD    + D   I   S   ++++ + C          L   TQI++PI + +A  +SI ++  ++K+    HP+ +Y+  +A FD++EL +Q GD++ EA+F IK+++AIQ L RS+CQ+LP  +N+ K     LY+ +A+DN +F+ + ++M+ +++ EWI NV I+ + +++T+NAQFFWTFDVT ND+  LH R++KR+F+C FSTT P    FT  IWP++DHL GE+LP L+ I     G L  + +   D  L+K  ++L L+ Q N +  II L   + +  R+ Y+  KY  +        S     K   ++ FS   N S   N E+ L+ IE  +K ++   ++ KF+IL+  +  FR  F
Sbjct:    1 MGRGAP-SKVGGIISNKDYRPKLFVLGKNVSILQFFQNGCFEKDLMSYCANRLSEYSCIYWLDENIENIDQDLIVVES---SDNTTVPCITISLSHFPNLQIATQILLPIFMFLAVMSSIFALIIMKKLAS-FHPLCSYLSIIAGFDLLELFVQSGDLLFEAFFNIKIISAIQFLKRSACQILPLIFNVLKQGQSALYIGLAVDNCRFITNARKMSISYRREWISNVCILASVLILTMNAQFFWTFDVTVNDNKLLHGRSVKRSFECGFSTTLPPNPNFTHFIWPLIDHLAGEILPVLIGIGSVIVGFLVLRKRLTVDPCLAKVTLDLSLILQTNFSFLIIVLCHSLVTFLRLCYYISKYILFLSYNNLSPSGIRG-KIAQSIRFSEKFNKSKLQNVEIVLEIIEYIIKLSNLALILAKFVILMFYLDNFRNHF 428
[Close]
BLAST of U3 small nucleolar RNA associated 22 vs. Planmine SMEST
Match: SMESG000078232.1 (SMESG000078232.1)

HSP 1 Score: 182.57 bits (462), Expect = 2.004e-50
Identity = 108/340 (31.76%), Postives = 183/340 (53.82%), Query Frame = 2 
Query:  248 TNLSLFEFIQEGCFEKDLMAYCSNRLNDYSCIHWLDPE------TXXXXXXXXXXXXXLFTEDSKILCYHDREDQSMYLMKLT-QIVIPIILLIAFATSILSIFAIRKITKKLHPIATYIIYLALFDIVELIIQGGDVIIEAYFRIKVLAAIQILSRSSCQMLPFAYNLFKHSNVILYMAMAIDNWKFVKHPKQMNDAFKSEWIRNVVIVIAAVLMTLNAQFFWTFDVTENDSINLHNRTLKRTFQCSFSTTWPLKQVFTECIWPILDHLVGEVLPSLVCICCGALYFQSKRVTDK-------NLSKYIINLDLVYQMNQALS---IISLLFGIFSLPRV 1216
            +NLS+FE +  GCF+ D   YC NR+N +SC +WL+ +           + +    S   + D+ I C     +   + + +   I+I  I+ +A   + L++F +RK+     PI  Y  Y+ +F+I    I   D +I  +   + L  +Q+LS+SSCQM+P  +++ +H    L + +AID W+FV+ P     +++SEW++NV I+   ++ T+N QFFWTFD+   ++   + R +    +C FS T PL  +FT  IWPI DH++ E+LP +  +   ++ F S  +  K       NLSK +IN  LV  + + L     +++LF +  LPR+
Sbjct:   28 SNLSVFEILINGCFDTDWETYCVNRINGFSCSYWLESDDFGNSINYGDKNYTAHGISIPISTDNNINCQSSSFNSENFSINIILDILITFIVTVAVVLNFLTLFTMRKLAHIYLPICHYFTYICVFEIFHCSILSLDWLISIFSTFQPLQILQLLSQSSCQMMPMLFSICEHLTSGLTIGIAIDLWRFVQCPVHFLGSYRSEWVKNVFIMCMVLVFTINCQFFWTFDLNHGENKFFNGRWISNYLECDFSNTLPLNPIFTRYIWPITDHVLSELLPLVTGL--FSVVFSSYLIHKKFKLKYPINLSKVLINAPLVLHLTKCLIGFLFVNILFNL--LPRI 363
[Close]
BLAST of U3 small nucleolar RNA associated 22 vs. Planmine SMEST
Match: SMESG000039629.1 (SMESG000039629.1)

HSP 1 Score: 56.225 bits (134), Expect = 4.743e-8
Identity = 51/245 (20.82%), Postives = 113/245 (46.12%), Query Frame = 2 
Query:  485 VIPIILLIAFATSILSIFAIRKITKKLHPIATYIIYLALFDIVELIIQGGDVIIEAYFRIKVLAAIQILSRSSCQMLPFAYNLFKHSNVILYMAMAIDNWKFVKHPKQMNDAFKSEWIRNVVIVIAAVLMTLNAQFFWTFDVTENDSINLHNR--TLKRTFQCSFSTTWPLKQVFTECIWPILDHLVGEVLPSLVCI--------------CCGALYFQSKRVTDKNLSKYIINLDLVYQMNQAL 1171
            ++PII+L +  T+I+ I  +  + +   P+  YI++L+  D++   ++  D   +  F  K L ++ + S + C        L  H   ++  A++ID +    +P           I +++++   +L+  N+ F WT+ +     I+++N+  ++     C++   W +   F + +WP++D  +GE+ P +  I              CC    F    + +K LS Y+I++    +M + +
Sbjct:   69 LLPIIILCSITTNIIMIRIMHNLAQWKFPMCLYIMFLSSLDLLSNTVKLLDKWCQITFSAKFLNSLVLTSHALCSTFTITIYLISHMISLVRSALSIDTYYSYINPLSNTRKIVVTRIHHLILLFTTLLLIFNSHFLWTYSI-----IHMNNKQTSVPPGNYCTYIVKW-VNPTFRDVLWPLVDFSIGELCPIICIIISTILILICLSKHSCCTENSFS---IENKKLSHYLIDVKTFMEMRKVI 304
The following BLAST results are available for this feature:
BLAST of U3 small nucleolar RNA associated 22 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of U3 small nucleolar RNA associated 22 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of U3 small nucleolar RNA associated 22 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of U3 small nucleolar RNA associated 22 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of U3 small nucleolar RNA associated 22 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of U3 small nucleolar RNA associated 22 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of U3 small nucleolar RNA associated 22 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of U3 small nucleolar RNA associated 22 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0X3NM382.674e-4828.49Uncharacterized protein OS=Schistocephalus solidus... [more]
A0A183SM839.095e-4828.95Uncharacterized protein OS=Schistocephalus solidus... [more]
A0A0X3NYT32.639e-4728.49Uncharacterized protein (Fragment) OS=Schistocepha... [more]
A0A3R7H7G01.781e-3932.63Uncharacterized protein OS=Clonorchis sinensis OX=... [more]
G7YLX81.802e-3932.63Uncharacterized protein OS=Clonorchis sinensis OX=... [more]
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BLAST of U3 small nucleolar RNA associated 22 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of U3 small nucleolar RNA associated 22 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of U3 small nucleolar RNA associated 22 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of U3 small nucleolar RNA associated 22 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of U3 small nucleolar RNA associated 22 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of U3 small nucleolar RNA associated 22 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000015428.10.000e+099.79SMESG000015428.1[more]
SMESG000079147.11.500e-9338.25SMESG000079147.1[more]
SMESG000078232.12.004e-5031.76SMESG000078232.1[more]
SMESG000039629.14.743e-820.82SMESG000039629.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027947 ID=SMED30027947|Name=U3 small nucleolar RNA associated 22|organism=Schmidtea mediterranea sexual|type=transcript|length=1874bp
GATCAACATTTATTTCTACTCTCACCGAAAATACTTTACTATTTGGTGCC
ATTCTGTCATGCCACTAGCAGAATTGACCAATTAAATTCAGTCGTATATA
TTCTGAAAAATTTCCCCAATCGATTTTAAAACTGATTTTGAAGCGACTTT
AGAAAATTTTTTAATCATGGGCCGCGGAGCTTCTCGAAGCAAATCAGGTG
GAATGACAGTAATGAAAGATTCAGAAAGAAAGATACTTTTAACTAATACC
AATTTGAGTTTGTTTGAATTTATCCAAGAGGGTTGTTTTGAAAAAGACTT
GATGGCTTATTGTTCAAATCGGCTCAATGATTATTCCTGCATTCATTGGT
TAGATCCAGAGACAAGTTCCAGTGACAGTCCTTCCATATCTTCAAATTCA
AGTTTATTTACGGAGGATTCAAAGATATTGTGCTACCATGACAGAGAAGA
TCAAAGTATGTACTTGATGAAGCTGACTCAAATTGTAATACCAATTATTT
TGCTAATAGCTTTTGCTACAAGTATTTTGTCAATATTTGCAATTAGAAAA
ATTACAAAAAAGCTCCATCCAATTGCAACATATATAATTTATTTAGCACT
TTTTGACATTGTAGAATTGATAATCCAAGGAGGTGATGTAATCATAGAAG
CGTATTTCAGAATAAAAGTATTGGCGGCCATTCAAATTCTCTCAAGATCA
TCGTGTCAAATGTTACCTTTTGCGTACAATTTGTTTAAGCATTCAAACGT
AATTCTCTACATGGCAATGGCAATTGACAATTGGAAATTTGTCAAACACC
CAAAACAAATGAATGACGCCTTCAAATCGGAATGGATAAGAAACGTTGTC
ATTGTGATTGCCGCAGTATTAATGACCCTTAATGCTCAATTCTTTTGGAC
GTTTGATGTGACTGAAAATGACAGCATCAATCTTCACAATCGAACTTTGA
AACGAACATTTCAATGCAGCTTTTCAACTACTTGGCCATTGAAACAAGTT
TTCACTGAATGCATTTGGCCGATTCTGGATCATTTAGTTGGTGAAGTTCT
TCCTTCCTTGGTTTGCATATGTTGTGGAGCTTTGTATTTCCAATCGAAAA
GAGTAACCGACAAAAACCTATCGAAATATATCATTAATCTCGATCTCGTT
TACCAAATGAATCAAGCGTTAAGCATTATTTCTTTGCTTTTTGGAATTTT
CTCTCTGCCGAGAGTGTTTTATTTTTCTTTCAAATACTTCTATTATTCCT
ATTTAAAATTTCATAAAAAAAGTTTTAGAGAATCTATGAAATTTAAATTT
AACATGAATTTCTCTCGGAATCTCAACTCATCCAACGAAGAAGTTTATTT
GCAGTACATCGAATTATTTGTCAAATACACCGATTTTCTCTTTATGATTG
TCAAATTTCTAATCTTGATTAAATTGGTTGGTTTGTTCAGAGTGAAATTT
GTCCAAATGTTCACATTTAATTCTAAAGGAAAATCGAATTTCGTTCCAAG
AAAATGTGACAAATGCTCAACGAAAGATTGCGACTGTTCTTTGCCGACTA
TTAAATTTGTACACAATTCTGTAGAACGGAAAATTCCTTCAGAAAATCTA
ACTCTTTGCTCATATACTATGTAAATATTCTCTCGAATGTCTTTTACAAT
TTAGATTTTCATATTTCTTTTCTAAAATAATCCAATGCCAATAAGCATGA
GAATCACTAAATGAAATAAAGAATCCATTTGTGACACAATTAGAAAAAAA
TGTCAGTAATGAATTTTTTTTGATATTTATTTGTAGACATAATGGGCTTA
GGAGAATAAAAATATTTGAGTAAAATCTTTTATCCGTTTCACCATTCATC
GATTTCTTTCACCTTCATTTCTTT
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protein sequence of SMED30027947-orf-1

>SMED30027947-orf-1 ID=SMED30027947-orf-1|Name=SMED30027947-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=486bp
MGRGASRSKSGGMTVMKDSERKILLTNTNLSLFEFIQEGCFEKDLMAYCS
NRLNDYSCIHWLDPETSSSDSPSISSNSSLFTEDSKILCYHDREDQSMYL
MKLTQIVIPIILLIAFATSILSIFAIRKITKKLHPIATYIIYLALFDIVE
LIIQGGDVIIEAYFRIKVLAAIQILSRSSCQMLPFAYNLFKHSNVILYMA
MAIDNWKFVKHPKQMNDAFKSEWIRNVVIVIAAVLMTLNAQFFWTFDVTE
NDSINLHNRTLKRTFQCSFSTTWPLKQVFTECIWPILDHLVGEVLPSLVC
ICCGALYFQSKRVTDKNLSKYIINLDLVYQMNQALSIISLLFGIFSLPRV
FYFSFKYFYYSYLKFHKKSFRESMKFKFNMNFSRNLNSSNEEVYLQYIEL
FVKYTDFLFMIVKFLILIKLVGLFRVKFVQMFTFNSKGKSNFVPRKCDKC
STKDCDCSLPTIKFVHNSVERKIPSENLTLCSYTM*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 91..440
e-value: 8.0E-10
score: 40.1
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 106..273
e-value: 2.84165E-6
score: 46.8572
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 74..365
NoneNo IPR availableTMHMMTMhelixcoord: 106..126
NoneNo IPR availableTMHMMTMhelixcoord: 407..429
NoneNo IPR availableTMHMMTMhelixcoord: 334..353
NoneNo IPR availableTMHMMTMhelixcoord: 136..158
NoneNo IPR availableTMHMMTMhelixcoord: 223..245
NoneNo IPR availableTMHMMTMhelixcoord: 286..308
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 118..394
score: 8.997