Tubulin beta chain

Overview
NameTubulin beta chain
Smed IDSMED30027929
Length (bp)1610
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Tubulin beta chain (SMED30027929) t-SNE clustered cells

Violin plots show distribution of expression levels for Tubulin beta chain (SMED30027929) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Tubulin beta chain (SMED30027929) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Tubulin beta chain (SMED30027929) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30027929

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 18

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30027929SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044907.1 SMESG000044888.1 SMESG000035804.1 SMESG000035755.1 Contig2059GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30027929SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044907.1 SMESG000044888.1 SMESG000035804.1 SMESG000035755.1 Contig2059GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
headSMED30027929SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044907.1 SMESG000044888.1 SMESG000035804.1 SMESG000035755.1 Contig2059GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
parapharyngeal regionSMED30027929 SMED30027929smed_20140614PMID:29906446
Zeng et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30027929 SMED30027929smed_20140614PMID:29906446
Zeng et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gamma neoblastSMED30027929 SMED30027929smed_20140614PMID:29906446
Zeng et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx progenitor cellSMED30027929 SMED30027929smed_20140614PMID:29906446
Zeng et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nu neoblastSMED30027929 SMED30027929smed_20140614PMID:29906446
Zeng et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30027929SMESG000022928.1 dd_Smed_v4_341_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
neuronSMED30027929SMESG000022928.1 dd_Smed_v4_341_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30027929SMESG000022928.1 dd_Smed_v4_341_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parapharyngeal cellSMED30027929SMESG000022928.1 dd_Smed_v4_341_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30027929SMESG000022928.1 dd_Smed_v4_471_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
neuronSMED30027929SMESG000022928.1 dd_Smed_v4_471_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30027929SMESG000022928.1 dd_Smed_v4_471_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30027929SMESG000022928.1 dd_Smed_v4_471_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parapharyngeal cellSMED30027929SMESG000022928.1 dd_Smed_v4_471_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30027929SMESG000047291.1 SMESG000047284.1 SMESG000046374.1 SMESG000044907.1 SMESG000044888.1 SMESG000035804.1 SMESG000035755.1 SMED_32051_V2_1rGPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB4B (tubulin beta 4B class IVb [Source:HGNC Symbol;Acc:HGNC:20771])

HSP 1 Score: 844.729 bits (2181), Expect = 0.000e+0
Identity = 417/427 (97.66%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB2B (tubulin beta 2B class IIb [Source:HGNC Symbol;Acc:HGNC:30829])

HSP 1 Score: 838.951 bits (2166), Expect = 0.000e+0
Identity = 411/427 (96.25%), Postives = 425/427 (99.53%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATG KYVPRA+LVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSV+PSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFD+KNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB (tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:20778])

HSP 1 Score: 837.025 bits (2161), Expect = 0.000e+0
Identity = 410/427 (96.02%), Postives = 425/427 (99.53%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQL+RI+VYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB (tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:20778])

HSP 1 Score: 837.025 bits (2161), Expect = 0.000e+0
Identity = 410/427 (96.02%), Postives = 425/427 (99.53%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQL+RI+VYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Human
Match: TUBB (tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:20778])

HSP 1 Score: 837.025 bits (2161), Expect = 0.000e+0
Identity = 410/427 (96.02%), Postives = 425/427 (99.53%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQL+RI+VYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-4 (Tubulin beta-4 chain [Source:UniProtKB/Swiss-Prot;Acc:P41937])

HSP 1 Score: 830.861 bits (2145), Expect = 0.000e+0
Identity = 405/427 (94.85%), Postives = 424/427 (99.30%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG Y+GDSDLQLERINVYYNEA+GGKYVPRA LVDLEPGTMDSVRAGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVD+VLD+VRKEAESCDCLQGFQ+THSLGGGTGSGMGTLLISKIREEYPDR+M+TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR+LTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRG+KM+ATF+GNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGAYNGDSDLQLERINVYYNEASGGKYVPRACLVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVVRKEAESCDCLQGFQMTHSLGGGTGSGMGTLLISKIREEYPDRIMMTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSMTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRSLTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGVKMAATFVGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 427          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: mec-7 (Tubulin beta-1 chain [Source:UniProtKB/Swiss-Prot;Acc:P12456])

HSP 1 Score: 810.831 bits (2093), Expect = 0.000e+0
Identity = 400/426 (93.90%), Postives = 415/426 (97.42%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 1404
            MREIVH+QAGQCGNQIGSKFWEVISDEHGIDP+G Y GDSDLQLERINVYYNEA   KYVPRAVLVDLEPGTMDSVR+GPFGQ+FRPDN++FGQSGAGNNWAKGHYTEGAELVD+VLD+VRKEAES DCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTD TFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSR +QQYRA+TVPELTQQ FDAKNMMAACDPRHGRYLT AAIFRGRMSMKEVDEQMLN+QNKNSSYFV+WIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ
Sbjct:    1 MREIVHIQAGQCGNQIGSKFWEVISDEHGIDPSGQYVGDSDLQLERINVYYNEAGSNKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNYVFGQSGAGNNWAKGHYTEGAELVDNVLDVVRKEAESTDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDSTFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRSNQQYRAITVPELTQQCFDAKNMMAACDPRHGRYLTAAAIFRGRMSMKEVDEQMLNIQNKNSSYFVDWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ 426          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: ben-1 (Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:Q18817])

HSP 1 Score: 809.675 bits (2090), Expect = 0.000e+0
Identity = 395/427 (92.51%), Postives = 417/427 (97.66%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIG+KFWEVISDEHGI P GTY G+SDLQLERINVYYNEA GGKYVPRAVLVDLEPGTMDSVR+GPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVD+VLD+VRKEAE CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M +FSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKL+ PTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPL+++G+Q YRALTV ELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSM+EVD+QM+NVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+
Sbjct:    1 MREIVHVQAGQCGNQIGAKFWEVISDEHGIQPDGTYKGESDLQLERINVYYNEANGGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVVRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMSSFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLSNPTYGDLNHLVSVTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLSAKGAQAYRALTVAELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMREVDDQMMNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 427          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-2 (Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot;Acc:P52275])

HSP 1 Score: 801.971 bits (2070), Expect = 0.000e+0
Identity = 389/427 (91.10%), Postives = 416/427 (97.42%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIGSKFWEVISDEHGI P GT+ G++DLQLERI+VYYNEA  GKYVPRAVLVDLEPGTMDSVR+GPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVD+VLD++RKEAE CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M +FSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDIC+RTLKLT PTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPL+++G+Q YRALTV ELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSM+EVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATF+GNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDL+SEYQQYQ+
Sbjct:    1 MREIVHVQAGQCGNQIGSKFWEVISDEHGIQPDGTFKGETDLQLERIDVYYNEANNGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVIRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMSSFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICYRTLKLTNPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLSAKGTQAYRALTVAELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMREVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFVGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLISEYQQYQE 427          
BLAST of Tubulin beta chain vs. Ensembl Celegans
Match: tbb-2 (Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot;Acc:P52275])

HSP 1 Score: 801.971 bits (2070), Expect = 0.000e+0
Identity = 389/427 (91.10%), Postives = 416/427 (97.42%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIGSKFWEVISDEHGI P GT+ G++DLQLERI+VYYNEA  GKYVPRAVLVDLEPGTMDSVR+GPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVD+VLD++RKEAE CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M +FSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDIC+RTLKLT PTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPL+++G+Q YRALTV ELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSM+EVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATF+GNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDL+SEYQQYQ+
Sbjct:    1 MREIVHVQAGQCGNQIGSKFWEVISDEHGIQPDGTFKGETDLQLERIDVYYNEANNGKYVPRAVLVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDNVLDVIRKEAEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMSSFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICYRTLKLTNPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLSAKGTQAYRALTVAELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMREVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFVGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLISEYQQYQE 427          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub56D (gene:FBgn0284243 transcript:FBtr0086536)

HSP 1 Score: 834.713 bits (2155), Expect = 0.000e+0
Identity = 409/427 (95.78%), Postives = 423/427 (99.06%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIG+KFWE+ISDEHGID TG YHGDSDLQLERINVYYNEA+GGKYVPRAVLVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M T+SVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLN+QNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDATGAYHGDSDLQLERINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 427          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub85D (gene:FBgn0003889 transcript:FBtr0082046)

HSP 1 Score: 824.313 bits (2128), Expect = 0.000e+0
Identity = 407/427 (95.32%), Postives = 419/427 (98.13%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIG KFWEVISDEH ID TGTY+GDSDLQLERINVYYNEATG KYVPRA+LVDLEPGTMDSVR+G FGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKE+E CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLN+QNKNSS+FVEWIPNN KTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+
Sbjct:    1 MREIVHIQAGQCGNQIGGKFWEVISDEHCIDATGTYYGDSDLQLERINVYYNEATGAKYVPRAILVDLEPGTMDSVRSGAFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESEGCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSFFVEWIPNNCKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 427          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub56D (gene:FBgn0284243 transcript:FBtr0086537)

HSP 1 Score: 793.882 bits (2049), Expect = 0.000e+0
Identity = 392/408 (96.08%), Postives = 404/408 (99.02%), Query Frame = 1
Query:  184 FWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            FWE+ISDEHGID TG YHGDSDLQLERINVYYNEA+GGKYVPRAVLVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M T+SVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLN+QNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+
Sbjct:   29 FWEIISDEHGIDATGAYHGDSDLQLERINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 436          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub60D (gene:FBgn0003888 transcript:FBtr0072270)

HSP 1 Score: 790.801 bits (2041), Expect = 0.000e+0
Identity = 385/433 (88.91%), Postives = 418/433 (96.54%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEAT------GGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIV+LQAGQCGNQIG+KFWE+IS+EHGID  G Y GDSDLQLER++VYYNEA+      GGKYVPRA+L+DLEPGTM+SVR+GP+GQ+FRPDNF++GQSGAGNNWAKGHYTEGAELVD+VLD+VRKE E+CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M T+SVVPSPKVSDTVVEPYNATLS+HQLVENTDET+CIDNEALYDICFRTLK++ P+YGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQML VQNKNSSYFVEWIPNN+KTAVCDIPP+GLKMS+TFIGN+TAIQELFKR+SEQF+AMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+
Sbjct:    1 MREIVNLQAGQCGNQIGAKFWEIISEEHGIDSNGIYVGDSDLQLERVSVYYNEASAVTRSSGGKYVPRAILLDLEPGTMESVRSGPYGQLFRPDNFVYGQSGAGNNWAKGHYTEGAELVDNVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKVSNPSYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLAVQNKNSSYFVEWIPNNVKTAVCDIPPKGLKMSSTFIGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 433          
BLAST of Tubulin beta chain vs. Ensembl Fly
Match: betaTub60D (gene:FBgn0003888 transcript:FBtr0343443)

HSP 1 Score: 790.801 bits (2041), Expect = 0.000e+0
Identity = 385/432 (89.12%), Postives = 418/432 (96.76%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEAT-----GGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIV+LQAGQCGNQIG+KFWE+IS+EHGID  G Y GDSDLQLER++VYYNEA+     GGKYVPRA+L+DLEPGTM+SVR+GP+GQ+FRPDNF++GQSGAGNNWAKGHYTEGAELVD+VLD+VRKE E+CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M T+SVVPSPKVSDTVVEPYNATLS+HQLVENTDET+CIDNEALYDICFRTLK++ P+YGDLNHLVS TMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQML VQNKNSSYFVEWIPNN+KTAVCDIPP+GLKMS+TFIGN+TAIQELFKR+SEQF+AMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQ+
Sbjct:    1 MREIVNLQAGQCGNQIGAKFWEIISEEHGIDSNGIYVGDSDLQLERVSVYYNEASVTRSSGGKYVPRAILLDLEPGTMESVRSGPYGQLFRPDNFVYGQSGAGNNWAKGHYTEGAELVDNVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTYSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKVSNPSYGDLNHLVSLTMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLAVQNKNSSYFVEWIPNNVKTAVCDIPPKGLKMSSTFIGNTTAIQELFKRISEQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQE 432          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 845.499 bits (2183), Expect = 0.000e+0
Identity = 415/427 (97.19%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVR+GPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 845.499 bits (2183), Expect = 0.000e+0
Identity = 415/427 (97.19%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVR+GPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 845.499 bits (2183), Expect = 0.000e+0
Identity = 415/427 (97.19%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVR+GPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: zgc:55461 (zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879])

HSP 1 Score: 845.114 bits (2182), Expect = 0.000e+0
Identity = 415/427 (97.19%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVR+GPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Match: tubb4b (tubulin, beta 4B class IVb [Source:ZFIN;Acc:ZDB-GENE-030131-8625])

HSP 1 Score: 843.573 bits (2178), Expect = 0.000e+0
Identity = 414/427 (96.96%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQL+RINVYYNEATGGKYVPRAVLVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLDRINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: reps2 (RALBP1 associated Eps domain containing 2 [Source:Xenbase;Acc:XB-GENE-985000])

HSP 1 Score: 847.04 bits (2187), Expect = 0.000e+0
Identity = 417/427 (97.66%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: ubxn11 (UBX domain protein 11 [Source:Xenbase;Acc:XB-GENE-985480])

HSP 1 Score: 838.951 bits (2166), Expect = 0.000e+0
Identity = 411/427 (96.25%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEA+GGKYVPRAVLVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSV+D+VRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR+LTVPELTQQMFD+KNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVMDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRSLTVPELTQQMFDSKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: znf330 (zinc finger protein 330 [Source:Xenbase;Acc:XB-GENE-1010548])

HSP 1 Score: 838.565 bits (2165), Expect = 0.000e+0
Identity = 411/427 (96.25%), Postives = 425/427 (99.53%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATG K+VPRA+LVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSV+PSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFD+KNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKFVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: tubb (tubulin beta class I [Source:NCBI gene;Acc:448742])

HSP 1 Score: 837.025 bits (2161), Expect = 0.000e+0
Identity = 410/427 (96.02%), Postives = 425/427 (99.53%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQL+RI+VYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Xenopus
Match: tubb3 (tubulin beta 3 class III [Source:NCBI gene;Acc:395045])

HSP 1 Score: 802.357 bits (2071), Expect = 0.000e+0
Identity = 393/427 (92.04%), Postives = 413/427 (96.72%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDP G Y GDSDLQLERI+VYYNEA+  KYVPRA+LVDLEPGTMDSVR+G FG +FRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKE E+CDCLQGFQLTHSLGGGTGSGMGTLLISK+REEYPDR+M TFSVVPSPKVSDTVVEPYNATLS+HQLVENTDET+CIDNEALYDICFRTLKL TPTYGDLNHLVSATMSGVTT LRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLT+RGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVA +FRGRMSMKEVDEQML +Q+KNSSYFVEWIPNN+K AVCDIPPRGLKMS+TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPCGNYIGDSDLQLERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVATVFRGRMSMKEVDEQMLAIQSKNSSYFVEWIPNNVKVAVCDIPPRGLKMSSTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb4b (tubulin, beta 4B class IVB [Source:MGI Symbol;Acc:MGI:1915472])

HSP 1 Score: 844.729 bits (2181), Expect = 0.000e+0
Identity = 417/427 (97.66%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb2b (tubulin, beta 2B class IIB [Source:MGI Symbol;Acc:MGI:1920960])

HSP 1 Score: 838.951 bits (2166), Expect = 0.000e+0
Identity = 411/427 (96.25%), Postives = 425/427 (99.53%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEATG KYVPRA+LVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSV+PSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFD+KNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEATGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb5 (tubulin, beta 5 class I [Source:MGI Symbol;Acc:MGI:107812])

HSP 1 Score: 837.025 bits (2161), Expect = 0.000e+0
Identity = 410/427 (96.02%), Postives = 425/427 (99.53%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQL+RI+VYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb4a (tubulin, beta 4A class IVA [Source:MGI Symbol;Acc:MGI:107848])

HSP 1 Score: 835.484 bits (2157), Expect = 0.000e+0
Identity = 411/427 (96.25%), Postives = 425/427 (99.53%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGG YVPRAVLVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVD+VLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREE+PDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQML+VQ+KNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGNYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEFPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLSVQSKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Mouse
Match: Tubb2a (tubulin, beta 2A class IIA [Source:MGI Symbol;Acc:MGI:107861])

HSP 1 Score: 833.943 bits (2153), Expect = 0.000e+0
Identity = 409/427 (95.78%), Postives = 423/427 (99.06%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEA G KYVPRA+LVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSV+PSPKVSDTVVEPYNATLSVHQLVENTDET+ IDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFD+KNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAAGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYSIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|P11833|TBB_PARLI (Tubulin beta chain OS=Paracentrotus lividus OX=7656 PE=2 SV=1)

HSP 1 Score: 847.04 bits (2187), Expect = 0.000e+0
Identity = 417/427 (97.66%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHMQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|P30883|TBB4_XENLA (Tubulin beta-4 chain OS=Xenopus laevis OX=8355 GN=tubb4 PE=2 SV=1)

HSP 1 Score: 846.269 bits (2185), Expect = 0.000e+0
Identity = 417/427 (97.66%), Postives = 425/427 (99.53%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG YHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGAYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|P68372|TBB4B_MOUSE (Tubulin beta-4B chain OS=Mus musculus OX=10090 GN=Tubb4b PE=1 SV=1)

HSP 1 Score: 844.729 bits (2181), Expect = 0.000e+0
Identity = 417/427 (97.66%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|P68371|TBB4B_HUMAN (Tubulin beta-4B chain OS=Homo sapiens OX=9606 GN=TUBB4B PE=1 SV=1)

HSP 1 Score: 844.729 bits (2181), Expect = 0.000e+0
Identity = 417/427 (97.66%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. UniProt/SwissProt
Match: sp|Q3MHM5|TBB4B_BOVIN (Tubulin beta-4B chain OS=Bos taurus OX=9913 GN=TUBB4B PE=2 SV=1)

HSP 1 Score: 844.729 bits (2181), Expect = 0.000e+0
Identity = 417/427 (97.66%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A2U9BBA2 (Ras GTPase-activating-like protein IQGAP1 OS=Scophthalmus maximus OX=52904 GN=SMAX5B_007995 PE=3 SV=1)

HSP 1 Score: 855.899 bits (2210), Expect = 0.000e+0
Identity = 411/427 (96.25%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG+YHGDSDLQL+RINVYYNEA+GGKYVPRAVLVDLEPGTMD+VR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTP+YGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYR+LTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLDRINVYYNEASGGKYVPRAVLVDLEPGTMDAVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPSYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRSLTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A267E855 (Tubulin beta chain (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig021796g2 PE=3 SV=1)

HSP 1 Score: 850.892 bits (2197), Expect = 0.000e+0
Identity = 418/428 (97.66%), Postives = 427/428 (99.77%), Query Frame = 1
Query:  124 KMREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            +MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMS+TFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:   33 RMREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSSTFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 460          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A1I8FZR8 (Tubulin beta chain OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 850.121 bits (2195), Expect = 0.000e+0
Identity = 418/427 (97.89%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMS+TFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:   61 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSSTFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 487          
BLAST of Tubulin beta chain vs. TrEMBL
Match: A0A480TFQ0 (Tubulin beta chain OS=Sus scrofa OX=9823 PE=3 SV=1)

HSP 1 Score: 847.425 bits (2188), Expect = 0.000e+0
Identity = 417/427 (97.66%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. TrEMBL
Match: W4XHC0 (Tubulin beta chain OS=Strongylocentrotus purpuratus OX=7668 PE=3 SV=1)

HSP 1 Score: 847.425 bits (2188), Expect = 0.000e+0
Identity = 418/427 (97.89%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDE+FCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDESFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: zgc:55461 (tubulin beta-4B chain [Source:NCBI gene;Acc:103024068])

HSP 1 Score: 843.573 bits (2178), Expect = 0.000e+0
Identity = 414/427 (96.96%), Postives = 425/427 (99.53%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTG YHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVR+GPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGAYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQVFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: TUBB2A (tubulin beta-2A chain [Source:NCBI gene;Acc:103041736])

HSP 1 Score: 824.698 bits (2129), Expect = 0.000e+0
Identity = 403/427 (94.38%), Postives = 422/427 (98.83%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIG+KFWEVISDEHGID TG+Y G+S+LQLERINVYYNEATG K+VPRA+LVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKE+E+CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSV+PSPKVSDTVVEPYNATLSVHQLVENTDETF IDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRAL+VPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQML+VQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELF+R+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:   78 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDATGSYQGESELQLERINVYYNEATGSKFVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETFSIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALSVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLSVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFRRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 504          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: TUBB1 (tubulin beta 1 class VI [Source:HGNC Symbol;Acc:HGNC:16257])

HSP 1 Score: 684.485 bits (1765), Expect = 0.000e+0
Identity = 342/427 (80.09%), Postives = 370/427 (86.65%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQ GQCGNQIGSK  E+      ID                        GGKYVPRA+LVDLEPGTMDSVR    GQ+FRPDNFI G SGAGNNWAKGHYTEGAELV+ V+D VR EAESCDCLQGFQ  HSLGGGTGSGMGTL+I+KIREEYPDR+M +FS++PSPKVSDTVVEPYNATLS+HQL+ENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVS TMSGVTT LRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLT+RGSQQYRALTVPELTQQMFDA+NMM ACDPR GRYLTVA IFRGRMS KEVDEQML +Q KNS+YFV+WIP+N+K AVCDIPPRGLKM++TFIGN+TAIQE+F+RV EQF  MFRRKAFLHWYTGEGMDEMEFTEAE+N+NDLVSEYQQYQD
Sbjct:    1 MREIVHLQIGQCGNQIGSKVTEI----RLID----------------------TCGGKYVPRALLVDLEPGTMDSVRGSRIGQLFRPDNFIHGNSGAGNNWAKGHYTEGAELVEQVVDRVRNEAESCDCLQGFQFVHSLGGGTGSGMGTLIINKIREEYPDRIMNSFSIMPSPKVSDTVVEPYNATLSIHQLIENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSLTMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDARNMMTACDPRRGRYLTVAGIFRGRMSTKEVDEQMLAIQQKNSNYFVDWIPHNVKVAVCDIPPRGLKMASTFIGNNTAIQEIFRRVGEQFAMMFRRKAFLHWYTGEGMDEMEFTEAENNLNDLVSEYQQYQD 401          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: ENSAMXT00000019550.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000026.1:1405913:1407004:-1 gene:ENSAMXG00000018987.2 transcript:ENSAMXT00000019550.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 647.892 bits (1670), Expect = 0.000e+0
Identity = 324/335 (96.72%), Postives = 333/335 (99.40%), Query Frame = 1
Query:  403 GQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            GQSGAGNNWAKGHYTEGAELVDSVLD+VRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:   12 GQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 346          
BLAST of Tubulin beta chain vs. Ensembl Cavefish
Match: ENSAMXT00000030346.1 (tubulin alpha-1B chain [Source:NCBI gene;Acc:111195381])

HSP 1 Score: 358.607 bits (919), Expect = 8.552e-119
Identity = 181/434 (41.71%), Postives = 266/434 (61.29%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQL--ERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPR--------GLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 1398
            MRE + +  GQ G QIG+  WE+   EHGI P G    D  +    +  N +++E   GK+VPRAV VDLEP  +D VR G + Q+F P+  I G+  A NN+A+GHYT G E++D VLD +RK A+ C  LQGF + HS GGGTGSG  +LL+ ++  +Y  +  L FS+ P+P+VS  VVEPYN+ L+ H  +E++D  F +DNEA+YDIC R L +  PTY +LN L+S  +S +T  LRF G LN DL +   N+VP+PR+HF +  +AP+ S     +  L+V E+T   F+  N M  CDPRHG+Y+    ++RG +  K+V+  +  ++ K +  FV+W P   K  +   PP          ++ +   + N+TAI E + R+  +F  M+ ++AF+HWY GEGM+E EF+EA  +M  L  +Y++
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEE 434          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: tubb2 (tubulin, beta 2A class IIa [Source:ZFIN;Acc:ZDB-GENE-050522-384])

HSP 1 Score: 706.442 bits (1822), Expect = 0.000e+0
Identity = 354/375 (94.40%), Postives = 367/375 (97.87%), Query Frame = 1
Query:  181 KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVV-PSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRK 1302
            +FWEVISDEHGIDPTG+YHGDSDLQLERINVYYNEA+GGKYVPRA+LVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEY  +V+ T     PSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFD+KNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRK
Sbjct:    1 QFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEASGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYIIQVLYTVVASGPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRK 375          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: TUBA3C (tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:12408])

HSP 1 Score: 299.671 bits (766), Expect = 5.904e-98
Identity = 152/348 (43.68%), Postives = 218/348 (62.64%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQL--ERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIP 1164
            MRE + +  GQ G QIG+  WE+   EHGI P G    D  +    +  N +++E   GK+VPRAV VDLEP  +D VR G + Q+F P+  I G+  A NN+A+GHYT G E+VD VLD +RK A+ C  LQGF + HS GGGTGSG  +LL+ ++  +Y  +  L F++ P+P+VS  VVEPYN+ L+ H  +E++D  F +DNEA+YDIC R L +  PTY +LN L+   +S +T  LRF G LN DL +   N+VP+PR+HF +  +AP+ S     +  L+V E+T   F+  N M  CDPRHG+Y+    ++RG +  K+V+  +  ++ K +  FV+W P
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCP 348          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009623.1 (pep scaffold:Pmarinus_7.0:GL485233:4234:10583:1 gene:ENSPMAG00000008696.1 transcript:ENSPMAT00000009623.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 251.136 bits (640), Expect = 6.679e-82
Identity = 135/159 (84.91%), Postives = 152/159 (95.60%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHG-DSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREE 600
            MREIVH+QAGQCGNQ+GSKFWEVIS+EHGID TG+Y G ++ LQ+ERINVYY+EAT GK+VPRA++VDLEPGTMD+VR+GP G +FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAE+CDCLQGFQLTHSLGGGTGSGMGTLLISKIREE
Sbjct:    1 MREIVHVQAGQCGNQVGSKFWEVISEEHGIDRTGSYRGYNARLQMERINVYYSEATSGKFVPRAIMVDLEPGTMDAVRSGPLGHMFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREE 159          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: tubg1 (tubulin, gamma 1 [Source:ZFIN;Acc:ZDB-GENE-040426-836])

HSP 1 Score: 258.455 bits (659), Expect = 8.926e-81
Identity = 145/439 (33.03%), Postives = 238/439 (54.21%), Query Frame = 1
Query:  130 REIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQ--SGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSP-KVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGS-QQYRALTVPELTQQMFDAKNMMAACDPRHGR-------YLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPP---RGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGM---DEMEFTEAESNMNDLVSEYQ 1395
            REI+ LQ GQCGNQIG +FW+ +  EHGI P G     +    +R +V++ +A    Y+PRAVL+DLEP  ++S+    +  ++ P+N    +   GAGNNWA GH ++G ++ + + DI+ +EA+  D L+             S      + K    +P +++ T+SV P+  ++SD VV+PYN+ L++ +L +N D    +DN AL  I    L +  P++  +N LVS  MS  TT LR+PG +N DL  L  +++P PRLHF M G+ PLT+  S    R  TV ++ +++   KN+M +     GR       Y+ +  I +G +   +V + +  ++ +  + F+ W P +I+ A+    P      ++S   + N T+I  LF R  +Q+  + +R+AFL  +  E M   D  EF ++   +  L+ EYQ
Sbjct:    3 REIITLQLGQCGNQIGFEFWKQLCAEHGISPDGILEDFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVINSIVNSSYANLYNPENIYLSEHGGGAGNNWASGH-SQGEKIHEEIFDIIDREADGSDSLE-----KCPLTRRLSATNWNELCKYFCRFPKKLIQTYSVFPNQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQPKNVMVST----GRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLTSAHRVSGLMMANHTSISSLFDRTCKQYDKLRKREAFLDQFRKEDMFKEDLSEFDDSREVVQQLIDEYQ 431          
BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001139.1 (pep scaffold:Pmarinus_7.0:GL478075:30403:34872:1 gene:ENSPMAG00000001018.1 transcript:ENSPMAT00000001139.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 233.802 bits (595), Expect = 2.019e-72
Identity = 128/351 (36.47%), Postives = 201/351 (57.26%), Query Frame = 1
Query:  130 REIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQL---ERINVYYNEATGGKYVPRAVLVDLEPGTMD---SVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIP 1164
            R+ + +  GQ G Q+G+  WE+   EHGI P G      D+        N +++E   GK+VPRA+ +DLEP  +     +R    G   R    + G+    +   +G    G ++   VL   R ++E C  LQGF L HS GGGTGSG  +LL+ ++  +Y  +  L F + P+P+VS  VVEPYNA L+ H  +E++D +F +DNEA+YDIC + L +  P+Y +LN L++  +S +T  LRF G LN DL +   N+VP+PR+HF +  +AP+ S     +  L+V E+T   F+  N M  CDPR+G+Y+    ++RG +  K+V+  +  ++++ +  FV+W P
Sbjct:    3 RDCISIHIGQAGVQMGNACWELYCLEHGIKPDGHLATHPDVTSVVDSSFNTFFSETGSGKHVPRAIFIDLEPSVIGEPCGLRTLTVGGXLRMSMMLAGREVRSSRIWRGQKGGGRKICSRVLRRNRPQSERCTGLQGFLLFHSFGGGTGSGFTSLLMERLSADYGKKSKLEFCIYPAPQVSTAVVEPYNAILTTHTTLEHSDCSFMVDNEAIYDICRKKLDIDFPSYFNLNRLIAQMVSSITASLRFDGALNVDLNEFQTNLVPYPRIHFPLTTYAPVISAEKAYHEQLSVAEITNACFEPSNQMVKCDPRNGKYMACCLLYRGDIVPKDVNTAIAIIKSRRAVRFVDWCP 353          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB2 (Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria [Source:SGD;Acc:S000001857])

HSP 1 Score: 683.33 bits (1762), Expect = 0.000e+0
Identity = 329/427 (77.05%), Postives = 384/427 (89.93%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREI+H+  GQCGNQIG+ FWE I  EHG+D  GTYHG  D+Q ER+NVY+NEA+ GK+VPR++ VDLEPGT+D+VR    G +FRPDN+IFGQS AGN WAKGHYTEGAELVDSV+D++R+EAE CD LQGFQ+THSLGGGTGSGMGTLLISKIREE+PDR+M TFSV+PSPK SDTVVEPYNATLSVHQLVE++DETFCIDNEALYDIC RTLKL  P+YGDLN+LVS+ MSGVTT LR+PGQLN+DLRKLAVN+VPFPRLHFFM G+APLT+ GSQ +R+LTVPELTQQMFDAKNMMAA DPR+GRYLTVAA FRG++S+KEV+++M  VQ+KNS YFVEWIPNN++TAVC + P+GL M+ATFI NST+IQELFKRV +QF+AMF+RKAFLHWYT EGMDE+EF+EAESNMNDLVSEYQQYQ+
Sbjct:    1 MREIIHISTGQCGNQIGAAFWETICGEHGLDFNGTYHGHDDIQKERLNVYFNEASSGKWVPRSINVDLEPGTIDAVRNSAIGNLFRPDNYIFGQSSAGNVWAKGHYTEGAELVDSVMDVIRREAEGCDSLQGFQITHSLGGGTGSGMGTLLISKIREEFPDRMMATFSVLPSPKTSDTVVEPYNATLSVHQLVEHSDETFCIDNEALYDICQRTLKLNQPSYGDLNNLVSSVMSGVTTSLRYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAPLTAIGSQSFRSLTVPELTQQMFDAKNMMAAADPRNGRYLTVAAFFRGKVSVKEVEDEMHKVQSKNSDYFVEWIPNNVQTAVCSVAPQGLDMAATFIANSTSIQELFKRVGDQFSAMFKRKAFLHWYTSEGMDELEFSEAESNMNDLVSEYQQYQE 427          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB1 (Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; relative distribution to nuclear foci increases upon DNA replication stress; TUB1 has a paralog, TUB3, that arose from the whole genome duplication [Source:SGD;Acc:S000004550])

HSP 1 Score: 351.673 bits (901), Expect = 4.768e-117
Identity = 182/435 (41.84%), Postives = 271/435 (62.30%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQL-----ERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRG--------LKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEY 1392
            MRE++ +  GQ G QIG+  WE+ S EHGI P G  H +  L       E  + +++E   GK+VPRA+ VDLEP  +D VR GP+  +F P+  I G+  A NN+A+GHYT G E++  VLD +RK A+ CD LQGF  THSLGGGTGSG+G+LL+ ++  EY  +  L F+V P+P+VS +VVEPYN  L+ H  +E+ D TF +DNEA+YD+C R L +  P++ +LN+L++  +S VT  LRF G LN DL +   N+VP+PR+HF +  ++P+ S+    + + +V E+T   F+  N M  CDPR G+Y+    ++RG +  ++V   +  V+NK +   V+W P   K  +C  PP          +  +   + N+T+I E +KR+  +F  M+ ++AF+HWY GEGM+E EFTEA  ++  L  +Y
Sbjct:    1 MREVISINVGQAGCQIGNACWELYSLEHGIKPDG--HLEDGLSKPKGGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRNGPYKDLFHPEQLISGKEDAANNYARGHYTVGREILGDVLDRIRKLADQCDGLQGFLFTHSLGGGTGSGLGSLLLEELSAEYGKKSKLEFAVYPAPQVSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDMCKRNLDIPRPSFANLNNLIAQVVSSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYSPVLSKSKAFHESNSVSEITNACFEPGNQMVKCDPRDGKYMATCLLYRGDVVTRDVQRAVEQVKNKKTVQLVDWCPTGFKIGICYEPPTATPNSQLATVDRAVCMLSNTTSIAEAWKRIDRKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALERDY 433          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB3 (Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication [Source:SGD;Acc:S000004593])

HSP 1 Score: 347.051 bits (889), Expect = 2.987e-115
Identity = 182/435 (41.84%), Postives = 269/435 (61.84%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQL-----ERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMS--------ATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEY 1392
            MRE++ +  GQ G QIG+  WE+ S EHGI   G  H +  L       E  + +++E   GK+VPRA+ VDLEP  +D VR G F ++F P+  I G+  A NN+A+GHYT G E+VD V + +RK A+ CD LQGF  THSLGGGTGSG+G+LL+  +  EY  +  L F+V P+P++S +VVEPYN  L+ H  +E+ D TF +DNEA+YDIC R L ++ P++ +LN L++  +S VT  LRF G LN DL +   N+VP+PR+HF +  +AP+ S+    + + +V E+T   F+  N M  CDP  G+Y+    ++RG +  ++V   +  V+NK +   V+W P   K  +C  PP  +  S           + N+TAI + +KR+ ++F  M+ ++AF+HWY GEGM+E EFTEA  ++  L  +Y
Sbjct:    1 MREVISINVGQAGCQIGNACWELYSLEHGIKEDG--HLEDGLSKPKGGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRTGRFKELFHPEQLINGKEDAANNYARGHYTVGREIVDEVEERIRKMADQCDGLQGFLFTHSLGGGTGSGLGSLLLENLSYEYGKKSKLEFAVYPAPQLSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDICKRNLGISRPSFSNLNGLIAQVISSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYAPILSKKRATHESNSVSEITNACFEPGNQMVKCDPTKGKYMANCLLYRGDVVTRDVQRAVEQVKNKKTVQMVDWCPTGFKIGICYEPPSVIPSSELANVDRAVCMLSNTTAIADAWKRIDQKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALERDY 433          
BLAST of Tubulin beta chain vs. Ensembl Yeast
Match: TUB4 (Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000004202])

HSP 1 Score: 169.859 bits (429), Expect = 2.404e-47
Identity = 128/440 (29.09%), Postives = 213/440 (48.41%), Query Frame = 1
Query:  133 EIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLER---INVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGA--GNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTS---RGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPN----NI--KTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYT-GEGMDEM-----EFTEAESNMNDLVSEY 1392
            EI+ LQAGQCGN +G   W  ++ EH I   G      D   ER      ++ E +  K+ PRA+++D EP  +  V    F   F P N      GA  GN+WA G Y  G    D +L+ + KE +S D  +GFQL HS+ GGTGSG+G+ L+  + + YP +++ T+SV P+ + S+ VV+ YN  L++ +L+E++D T   DN +L +I  +  +         N L+S  +S VT  +RFP  + + +  +   ++P P LHF  P F P TS        ++  +  ++   + D  N + +    +  Y  V     G +  +++   M  +Q +    F  W  +    NI  ++    + P   ++S   + N + +  +F+     F  +F + AFL+ Y  G+    M     EF E+   +  L+ +Y
Sbjct:    4 EIITLQAGQCGNHVGKFLWSQLAKEHAIGTDGLSQL-PDSSTERDDDTKPFFRENSRNKFTPRAIMMDSEPSVIADVE-NTFRGFFDPRNTWVASDGASAGNSWANG-YDIGTRNQDDILNKIDKEIDSTDNFEGFQLLHSVAGGTGSGLGSNLLEALCDRYPKKILTTYSVFPA-RSSEVVVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRNPNIDLQHTNQLISTIISSVTNSIRFPSYMYSSMSSIYSTLIPSPELHFLSPSFTPFTSDYIHDDIAHKGHSSYDVMLDLLDPSNSLVSTAMNNPTYFNVYNTIIGNVEPRQISRAMTKLQQR--IKFPSWSSSAMHVNIGRRSPYLPLQPNENEVSGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNYNVGDLFQSMQNVQDEFAESREVVQSLMEDY 437          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO39945 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S8I3])

HSP 1 Score: 843.188 bits (2177), Expect = 0.000e+0
Identity = 415/427 (97.19%), Postives = 425/427 (99.53%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHCQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO35958 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJX9])

HSP 1 Score: 837.41 bits (2162), Expect = 0.000e+0
Identity = 411/427 (96.25%), Postives = 425/427 (99.53%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIV LQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPR+VL+DLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAEL+DSVLD+VRKEAESCDC+QGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFS+VPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVSLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRSVLIDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELMDSVLDVVRKEAESCDCIQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSIVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO42890 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S027])

HSP 1 Score: 820.846 bits (2119), Expect = 0.000e+0
Identity = 406/427 (95.08%), Postives = 418/427 (97.89%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFW    +EHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDC+QGFQLTHSLGGGTGSGM TLLI KIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRL FFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDP HGRYLTVAA+FRGRMSMKE+DEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFW----NEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCIQGFQLTHSLGGGTGSGMVTLLILKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLPFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPLHGRYLTVAAMFRGRMSMKELDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 423          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO49518 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGG3])

HSP 1 Score: 680.633 bits (1755), Expect = 0.000e+0
Identity = 324/429 (75.52%), Postives = 376/429 (87.65%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYH--GDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREI+++Q GQCGNQIG++FW  I++EHGIDPTG      D+  + +RI+VY++E  GG+YVPR++LVDLEPGTMD+VR GP G +FRPDNF+  QSGA NNWAKGHY+EGAEL+D++ D++RKEAE+CDC+QG QL  +LGGGTGSG+GTL++SKIREEYPDR++  FSVVPSPKVS+TVVEPYNATLSVHQLVE  DE F  DNEALYDIC RTLKL  P Y DLN LVS  M G+T  LRFPGQLN DLRKLAVNMVPFPRLHFFMPGFAPL SR +QQY+ ++V ELTQQMFDA+NMM ACDPRHGRYLTVAAIFRG+MSM+EVDEQMLN QNKNS +FVEWIPNN+KT VCDI P+GLKM+ATF+GN+TAIQELFKRV EQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNM+DL++EYQQYQ+
Sbjct:    1 MREIINIQVGQCGNQIGTEFWRDIAEEHGIDPTGAKSLIEDTHGRFDRIDVYFHEGNGGRYVPRSILVDLEPGTMDAVRGGPLGNMFRPDNFVNSQSGAANNWAKGHYSEGAELIDNIFDVLRKEAENCDCMQGVQLIQALGGGTGSGLGTLILSKIREEYPDRILSAFSVVPSPKVSETVVEPYNATLSVHQLVEVVDEDFMFDNEALYDICCRTLKLAKPNYADLNQLVSMAMCGLTASLRFPGQLNGDLRKLAVNMVPFPRLHFFMPGFAPLASRANQQYQNVSVEELTQQMFDARNMMTACDPRHGRYLTVAAIFRGKMSMREVDEQMLNTQNKNSPFFVEWIPNNVKTTVCDIAPKGLKMAATFVGNNTAIQELFKRVGEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMHDLIAEYQQYQE 429          
BLAST of Tubulin beta chain vs. Ensembl Nematostella
Match: EDO36734 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHY4])

HSP 1 Score: 654.44 bits (1687), Expect = 0.000e+0
Identity = 328/337 (97.33%), Postives = 335/337 (99.41%), Query Frame = 1
Query:  397 IFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            + GQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MLGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAMFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 337          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: tubb5 (tubulin beta chain [Source:NCBI gene;Acc:101157969])

HSP 1 Score: 834.328 bits (2154), Expect = 0.000e+0
Identity = 409/427 (95.78%), Postives = 425/427 (99.53%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQL+RI+VYYNEATGGKYVPRA+LVDLEPGTMDSVR+GPFGQIFRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLD+VRKE+ESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDET+CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKM+ TFIGNSTAIQELFKR+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: tubb2 (tubulin beta-2B chain [Source:NCBI gene;Acc:101162245])

HSP 1 Score: 828.55 bits (2139), Expect = 0.000e+0
Identity = 405/427 (94.85%), Postives = 424/427 (99.30%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIG+KFWEVISDEHGIDPTG+Y GDSDLQLERINVYYNEA+G KYVPRA+LVDLEPGTMDSVR+GPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVD+VLD+VRKE+E+CDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSV+PSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRAL+VPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSM+EVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELF+R+SEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYQGDSDLQLERINVYYNEASGSKYVPRAILVDLEPGTMDSVRSGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKESENCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALSVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMREVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFRRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: ENSORLT00000045809.1 (tubulin beta-6 chain [Source:NCBI gene;Acc:101166121])

HSP 1 Score: 739.184 bits (1907), Expect = 0.000e+0
Identity = 357/427 (83.61%), Postives = 394/427 (92.27%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+Q GQCGNQIGSKFWEVIS+EHGI+ TG Y GDS LQLERINVY++EA GGKYVPRA+LVDLEPGTMDS+R    G +FRPDNFI G SGAGNNWAKGHYTEGAELV+ V+D VR E+E CDCLQGFQL HSLGGGTGSGMGTL+I+KI+EE+PDR+M TFS++PSPKVSDTVVEPYNATLSVHQL+EN D TFCIDNEALYDICFRTLKLTTPTYGDLNHLV  TMSGVTT LRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLT+RGSQQYRALTVPELTQQMFDA+NMM ACDPR GRYLTVA IFRG+MS KEVDEQML +Q KNS+YFV+WIP+N+K AVCDIPP+GL+M++TFIGN+TAIQE+F+RV EQF+ MFRRKAFLHWYTGEGMDEMEFTEAESN+NDLVSEYQQYQ+
Sbjct:    1 MREIVHVQIGQCGNQIGSKFWEVISEEHGINATGIYEGDSTLQLERINVYFSEAYGGKYVPRALLVDLEPGTMDSIRGSRIGALFRPDNFIHGNSGAGNNWAKGHYTEGAELVEQVVDRVRHESEGCDCLQGFQLVHSLGGGTGSGMGTLIINKIKEEFPDRIMNTFSIMPSPKVSDTVVEPYNATLSVHQLLENADATFCIDNEALYDICFRTLKLTTPTYGDLNHLVCMTMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDARNMMTACDPRRGRYLTVAGIFRGKMSTKEVDEQMLAIQQKNSTYFVDWIPHNVKVAVCDIPPKGLRMASTFIGNNTAIQEIFRRVGEQFSLMFRRKAFLHWYTGEGMDEMEFTEAESNLNDLVSEYQQYQE 427          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: tuba1c (tubulin alpha-1B chain-like [Source:NCBI gene;Acc:101167538])

HSP 1 Score: 360.918 bits (925), Expect = 1.056e-119
Identity = 183/434 (42.17%), Postives = 266/434 (61.29%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQL--ERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPR--------GLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 1398
            MRE + +  GQ G QIG+  WE+   EHGI P G    D  L    +  N +++E   GK+VPRAV VDLEP  +D VR G + Q+F P+  I G+  A NN+A+GHYT G E++D VLD +RK A+ C  LQGF + HS GGGTGSG  +LL+ ++  +Y  +  L FS+ P+P+VS  VVEPYN+ L+ H  +E++D  F +DNEA+YDIC R L +  PTY +LN L+S  +S +T  LRF G LN DL +   N+VP+PR+HF +  +AP+ S     +  L+V E+T   F+  N M  CDPRHG+Y+    +FRG +  K+V+  +  ++ K +  FV+W P   K  +   PP          ++ +   + N+TAI E + R+  +F  M+ ++AF+HWY GEGM+E EF+EA  +M  L  +Y++
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTLGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLFRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEE 434          
BLAST of Tubulin beta chain vs. Ensembl Medaka
Match: ENSORLT00000033163.1 (tubulin alpha-1A chain [Source:NCBI gene;Acc:105354455])

HSP 1 Score: 360.533 bits (924), Expect = 1.444e-119
Identity = 183/434 (42.17%), Postives = 266/434 (61.29%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQL--ERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPR--------GLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQ 1398
            MRE + +  GQ G QIG+  WE+   EHGI P G    D  +    +  N +++E   GK+VPRAV VDLEP  +D VR G + Q+F P+  I G+  A NN+A+GHYT G E++D VLD +RK A+ C  LQGF + HS GGGTGSG  +LL+ ++  +Y  +  L FS+ P+P+VS  VVEPYNA L+ H  +E++D  F +DNEA+YDIC R L +  PTY +LN L+S  +S +T  LRF G LN DL +   N+VP+PR+HF +  +AP+ S     +  L+V E+T   F+  N M  CDPRHG+Y+    ++RG +  K+V+  +  ++ K S  FV+W P   K  +   PP          ++ +   + N+TAI E + R+  +F  M+ ++AF+HWY GEGM+E EF+EA  +M  L  +Y++
Sbjct:    1 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNAILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEE 434          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000022928.1 (SMESG000022928.1)

HSP 1 Score: 851.662 bits (2199), Expect = 0.000e+0
Identity = 424/427 (99.30%), Postives = 427/427 (100.00%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQ+FDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMS+TFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQIFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSSTFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000050810.1 (SMESG000050810.1)

HSP 1 Score: 850.121 bits (2195), Expect = 0.000e+0
Identity = 423/427 (99.06%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQ+FRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLD+VRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000047284.1 (SMESG000047284.1)

HSP 1 Score: 849.351 bits (2193), Expect = 0.000e+0
Identity = 421/427 (98.59%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000046374.1 (SMESG000046374.1)

HSP 1 Score: 849.351 bits (2193), Expect = 0.000e+0
Identity = 421/427 (98.59%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVH+QAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHMQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
BLAST of Tubulin beta chain vs. Planmine SMEST
Match: SMESG000035755.1 (SMESG000035755.1)

HSP 1 Score: 848.195 bits (2190), Expect = 0.000e+0
Identity = 419/427 (98.13%), Postives = 426/427 (99.77%), Query Frame = 1
Query:  127 MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 1407
            MREIVHLQAGQCGNQIG+KFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVL+DLEPGTMDSVRAGPFGQ+FRPDNF+FGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDR+M TFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAA+FRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNN+KTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD
Sbjct:    1 MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGKYVPRAVLIDLEPGTMDSVRAGPFGQLFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAALFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQD 427          
The following BLAST results are available for this feature:
BLAST of Tubulin beta chain vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TUBB4B0.000e+097.66tubulin beta 4B class IVb [Source:HGNC Symbol;Acc:... [more]
TUBB2B0.000e+096.25tubulin beta 2B class IIb [Source:HGNC Symbol;Acc:... [more]
TUBB0.000e+096.02tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:... [more]
TUBB0.000e+096.02tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:... [more]
TUBB0.000e+096.02tubulin beta class I [Source:HGNC Symbol;Acc:HGNC:... [more]
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BLAST of Tubulin beta chain vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tbb-40.000e+094.85Tubulin beta-4 chain [Source:UniProtKB/Swiss-Prot... [more]
mec-70.000e+093.90Tubulin beta-1 chain [Source:UniProtKB/Swiss-Prot... [more]
ben-10.000e+092.51Tubulin beta chain [Source:UniProtKB/TrEMBL;Acc:Q... [more]
tbb-20.000e+091.10Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot... [more]
tbb-20.000e+091.10Tubulin beta-2 chain [Source:UniProtKB/Swiss-Prot... [more]
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BLAST of Tubulin beta chain vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
betaTub56D0.000e+095.78gene:FBgn0284243 transcript:FBtr0086536[more]
betaTub85D0.000e+095.32gene:FBgn0003889 transcript:FBtr0082046[more]
betaTub56D0.000e+096.08gene:FBgn0284243 transcript:FBtr0086537[more]
betaTub60D0.000e+088.91gene:FBgn0003888 transcript:FBtr0072270[more]
betaTub60D0.000e+089.12gene:FBgn0003888 transcript:FBtr0343443[more]
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BLAST of Tubulin beta chain vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
zgc:554610.000e+097.19zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
zgc:554610.000e+097.19zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
zgc:554610.000e+097.19zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
zgc:554610.000e+097.19zgc:55461 [Source:ZFIN;Acc:ZDB-GENE-040426-2879][more]
tubb4b0.000e+096.96tubulin, beta 4B class IVb [Source:ZFIN;Acc:ZDB-GE... [more]
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BLAST of Tubulin beta chain vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
reps20.000e+097.66RALBP1 associated Eps domain containing 2 [Source:... [more]
ubxn110.000e+096.25UBX domain protein 11 [Source:Xenbase;Acc:XB-GENE-... [more]
znf3300.000e+096.25zinc finger protein 330 [Source:Xenbase;Acc:XB-GEN... [more]
tubb0.000e+096.02tubulin beta class I [Source:NCBI gene;Acc:448742][more]
tubb30.000e+092.04tubulin beta 3 class III [Source:NCBI gene;Acc:395... [more]
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BLAST of Tubulin beta chain vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tubb4b0.000e+097.66tubulin, beta 4B class IVB [Source:MGI Symbol;Acc:... [more]
Tubb2b0.000e+096.25tubulin, beta 2B class IIB [Source:MGI Symbol;Acc:... [more]
Tubb50.000e+096.02tubulin, beta 5 class I [Source:MGI Symbol;Acc:MGI... [more]
Tubb4a0.000e+096.25tubulin, beta 4A class IVA [Source:MGI Symbol;Acc:... [more]
Tubb2a0.000e+095.78tubulin, beta 2A class IIA [Source:MGI Symbol;Acc:... [more]
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BLAST of Tubulin beta chain vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P11833|TBB_PARLI0.000e+097.66Tubulin beta chain OS=Paracentrotus lividus OX=765... [more]
sp|P30883|TBB4_XENLA0.000e+097.66Tubulin beta-4 chain OS=Xenopus laevis OX=8355 GN=... [more]
sp|P68372|TBB4B_MOUSE0.000e+097.66Tubulin beta-4B chain OS=Mus musculus OX=10090 GN=... [more]
sp|P68371|TBB4B_HUMAN0.000e+097.66Tubulin beta-4B chain OS=Homo sapiens OX=9606 GN=T... [more]
sp|Q3MHM5|TBB4B_BOVIN0.000e+097.66Tubulin beta-4B chain OS=Bos taurus OX=9913 GN=TUB... [more]
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BLAST of Tubulin beta chain vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2U9BBA20.000e+096.25Ras GTPase-activating-like protein IQGAP1 OS=Scoph... [more]
A0A267E8550.000e+097.66Tubulin beta chain (Fragment) OS=Macrostomum ligna... [more]
A0A1I8FZR80.000e+097.89Tubulin beta chain OS=Macrostomum lignano OX=28230... [more]
A0A480TFQ00.000e+097.66Tubulin beta chain OS=Sus scrofa OX=9823 PE=3 SV=1[more]
W4XHC00.000e+097.89Tubulin beta chain OS=Strongylocentrotus purpuratu... [more]
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BLAST of Tubulin beta chain vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
zgc:554610.000e+096.96tubulin beta-4B chain [Source:NCBI gene;Acc:103024... [more]
TUBB2A0.000e+094.38tubulin beta-2A chain [Source:NCBI gene;Acc:103041... [more]
TUBB10.000e+080.09tubulin beta 1 class VI [Source:HGNC Symbol;Acc:HG... [more]
ENSAMXT00000019550.20.000e+096.72pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000030346.18.552e-11941.71tubulin alpha-1B chain [Source:NCBI gene;Acc:11119... [more]
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BLAST of Tubulin beta chain vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tubb20.000e+094.40tubulin, beta 2A class IIa [Source:ZFIN;Acc:ZDB-GE... [more]
TUBA3C5.904e-9843.68tubulin alpha 3c [Source:HGNC Symbol;Acc:HGNC:1240... [more]
ENSPMAT00000009623.16.679e-8284.91pep scaffold:Pmarinus_7.0:GL485233:4234:10583:1 ge... [more]
tubg18.926e-8133.03tubulin, gamma 1 [Source:ZFIN;Acc:ZDB-GENE-040426-... [more]
ENSPMAT00000001139.12.019e-7236.47pep scaffold:Pmarinus_7.0:GL478075:30403:34872:1 g... [more]
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BLAST of Tubulin beta chain vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 4
Match NameE-valueIdentityDescription
TUB20.000e+077.05Beta-tubulin; associates with alpha-tubulin (Tub1p... [more]
TUB14.768e-11741.84Alpha-tubulin; associates with beta-tubulin (Tub2p... [more]
TUB32.987e-11541.84Alpha-tubulin; associates with beta-tubulin (Tub2p... [more]
TUB42.404e-4729.09Gamma-tubulin; involved in nucleating microtubules... [more]
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BLAST of Tubulin beta chain vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO399450.000e+097.19Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO359580.000e+096.25Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO428900.000e+095.08Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO495180.000e+075.52Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO367340.000e+097.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Tubulin beta chain vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tubb50.000e+095.78tubulin beta chain [Source:NCBI gene;Acc:101157969... [more]
tubb20.000e+094.85tubulin beta-2B chain [Source:NCBI gene;Acc:101162... [more]
ENSORLT00000045809.10.000e+083.61tubulin beta-6 chain [Source:NCBI gene;Acc:1011661... [more]
tuba1c1.056e-11942.17tubulin alpha-1B chain-like [Source:NCBI gene;Acc:... [more]
ENSORLT00000033163.11.444e-11942.17tubulin alpha-1A chain [Source:NCBI gene;Acc:10535... [more]
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BLAST of Tubulin beta chain vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000022928.10.000e+099.30SMESG000022928.1[more]
SMESG000050810.10.000e+099.06SMESG000050810.1[more]
SMESG000047284.10.000e+098.59SMESG000047284.1[more]
SMESG000046374.10.000e+098.59SMESG000046374.1[more]
SMESG000035755.10.000e+098.13SMESG000035755.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027929 ID=SMED30027929|Name=Tubulin beta chain|organism=Schmidtea mediterranea sexual|type=transcript|length=1610bp
GTTTGCAGTTGCATGTATGTAATAGTAATGAGATTCTTTCTCTCCATGTA
TACACATGTATAAATAAATTCATGCCTCCGCCTTCGCCATTTTGAATTTT
CAACAAATCTTCTAAAAAAGTAGAAAATGCGTGAGATTGTTCACTTGCAA
GCAGGTCAGTGTGGAAATCAAATTGGATCAAAGTTTTGGGAAGTTATATC
TGATGAACATGGCATTGATCCAACTGGAACTTACCATGGGGATTCCGATT
TACAATTAGAAAGAATCAATGTTTATTATAATGAAGCAACTGGAGGAAAA
TATGTTCCAAGAGCTGTTTTAGTTGATTTAGAACCTGGTACAATGGATTC
TGTCAGAGCAGGACCTTTCGGTCAGATATTTAGACCTGATAATTTTATTT
TTGGCCAAAGTGGTGCAGGAAACAATTGGGCTAAAGGACATTATACCGAA
GGAGCTGAACTTGTGGATTCCGTTTTAGATATAGTACGAAAAGAAGCCGA
ATCATGTGATTGCTTGCAAGGATTTCAATTGACCCATTCTCTTGGAGGTG
GAACCGGATCGGGAATGGGGACATTATTAATTTCTAAAATAAGGGAAGAA
TATCCCGATCGTGTTATGTTAACGTTTTCAGTTGTACCATCACCAAAAGT
ATCCGACACCGTTGTTGAACCTTATAATGCAACACTTTCTGTTCATCAAT
TAGTAGAAAACACTGATGAAACTTTCTGCATTGATAACGAAGCACTTTAC
GATATTTGCTTTAGAACATTGAAGCTAACAACTCCTACTTATGGGGATTT
AAATCATTTAGTTAGTGCAACAATGAGTGGAGTGACAACTTGTTTACGAT
TTCCTGGTCAATTGAATGCCGATTTAAGAAAATTAGCTGTAAACATGGTT
CCATTCCCTCGTTTGCATTTCTTTATGCCGGGATTTGCTCCATTGACCAG
TCGAGGAAGTCAACAATATCGTGCATTGACCGTTCCAGAATTAACTCAAC
AAATGTTCGATGCCAAGAACATGATGGCTGCTTGTGATCCAAGACATGGT
CGTTATTTGACTGTTGCGGCAATTTTCCGTGGTCGCATGTCTATGAAAGA
AGTTGATGAGCAAATGTTGAATGTTCAAAATAAGAATAGTAGTTATTTTG
TTGAATGGATTCCCAACAATATTAAAACCGCGGTTTGTGATATTCCACCA
AGAGGATTAAAGATGTCTGCCACATTTATTGGTAATAGCACTGCTATTCA
AGAGCTATTCAAGCGTGTCAGTGAACAATTTACCGCTATGTTCCGTCGAA
AGGCTTTCTTGCATTGGTATACTGGTGAAGGTATGGATGAGATGGAGTTC
ACAGAAGCTGAATCTAATATGAATGACTTGGTTAGTGAATATCAGCAATA
TCAAGATGCAACGGCTGAAGAAGAAGGCGAATTCGAAGAGGAAGAAGCCG
AACAATAATTGCCATTTTGATTAAACAATTTTCCTTATGTTGTTATTTTG
AGTTTAATAAATAGAAAATTTTCACAAATGTATTTTGAACAATTCTTTTA
GTAAATATAAATTTATGGATAATATTTTGTGATTAAATGTAATTGGATAC
TTGCCAGTTA
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protein sequence of SMED30027929-orf-1

>SMED30027929-orf-1 ID=SMED30027929-orf-1|Name=SMED30027929-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=444bp
MREIVHLQAGQCGNQIGSKFWEVISDEHGIDPTGTYHGDSDLQLERINVY
YNEATGGKYVPRAVLVDLEPGTMDSVRAGPFGQIFRPDNFIFGQSGAGNN
WAKGHYTEGAELVDSVLDIVRKEAESCDCLQGFQLTHSLGGGTGSGMGTL
LISKIREEYPDRVMLTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETF
CIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADL
RKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMM
AACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNIK
TAVCDIPPRGLKMSATFIGNSTAIQELFKRVSEQFTAMFRRKAFLHWYTG
EGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEGEFEEEEAEQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000026gut
PLANA:0000039gamma neoblast
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000110pharynx progenitor cell
PLANA:0000418head
PLANA:0000420parapharyngeal region
PLANA:0000429neoblast
PLANA:0003116parenchymal cell
PLANA:0003117parapharyngeal cell
PLANA:0007552nu neoblast
Vocabulary: INTERPRO
TermDefinition
IPR037103Tubulin/FtsZ_C_sf
IPR036525Tubulin/FtsZ_GTPase_sf
IPR013838Beta-tubulin_BS
IPR000217Tubulin
IPR002453Beta_tubulin
IPR018316Tubulin/FtsZ_2-layer-sand-dom
IPR003008Tubulin_FtsZ_GTPase
IPR023123Tubulin_C
IPR017975Tubulin_CS
IPR008280Tub_FtsZ_C
Vocabulary: biological process
TermDefinition
GO:0007017microtubule-based process
GO:0007010cytoskeleton organization
Vocabulary: cellular component
TermDefinition
GO:0005874microtubule
GO:0005634nucleus
GO:0005737cytoplasm
GO:0005856cytoskeleton
GO:0015630microtubule cytoskeleton
GO:0031012extracellular matrix
GO:0043209myelin sheath
GO:0045298tubulin complex
GO:0070062extracellular exosome
GO:1903561extracellular vesicle
Vocabulary: molecular function
TermDefinition
GO:0005525GTP binding
GO:0005200structural constituent of cytoskeleton
GO:0003924GTPase activity
GO:0000166nucleotide binding
GO:0003725double-stranded RNA binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 408..442
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 422..443
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 428..443
NoneNo IPR availablePANTHERPTHR11588:SF340TUBULIN BETA CHAINcoord: 2..440
NoneNo IPR availableCDDcd02187beta_tubulincoord: 2..426
e-value: 0.0
score: 900.008
IPR000217TubulinPRINTSPR01161TUBULINcoord: 51..70
score: 71.21
coord: 10..30
score: 72.61
coord: 370..398
score: 67.67
coord: 151..172
score: 58.45
coord: 190..210
score: 69.69
coord: 106..130
score: 68.06
coord: 93..104
score: 76.26
coord: 132..150
score: 79.22
coord: 176..189
score: 79.11
IPR000217TubulinPANTHERPTHR11588TUBULINcoord: 2..440
IPR002453Beta tubulinPRINTSPR01163BETATUBULINcoord: 230..239
score: 83.43
coord: 41..58
score: 81.78
coord: 88..99
score: 88.81
coord: 412..430
score: 93.14
coord: 347..370
score: 82.0
coord: 377..388
score: 83.43
coord: 152..164
score: 87.52
coord: 212..224
score: 85.45
coord: 245..258
score: 89.06
coord: 329..343
score: 89.56
coord: 311..329
score: 79.49
coord: 108..126
score: 83.28
coord: 265..281
score: 85.71
IPR018316Tubulin/FtsZ, 2-layer sandwich domainSMARTSM00865Tubulin_C_4coord: 246..383
e-value: 3.5E-50
score: 182.7
IPR018316Tubulin/FtsZ, 2-layer sandwich domainPFAMPF03953Tubulin_Ccoord: 261..382
e-value: 1.1E-42
score: 145.1
IPR003008Tubulin/FtsZ, GTPase domainSMARTSM00864Tubulin_4coord: 47..244
e-value: 1.0E-70
score: 250.9
IPR003008Tubulin/FtsZ, GTPase domainPFAMPF00091Tubulincoord: 3..211
e-value: 1.3E-69
score: 234.4
IPR037103Tubulin/FtsZ, C-terminal domain superfamilyGENE3DG3DSA:3.30.1330.20coord: 264..373
e-value: 6.9E-57
score: 192.5
IPR023123Tubulin, C-terminalGENE3DG3DSA:1.10.287.600coord: 374..443
e-value: 1.1E-35
score: 123.4
IPR036525Tubulin/FtsZ, GTPase domain superfamilyGENE3DG3DSA:3.40.50.1440coord: 1..263
e-value: 2.4E-129
score: 432.5
IPR036525Tubulin/FtsZ, GTPase domain superfamilySUPERFAMILYSSF52490Tubulin nucleotide-binding domain-likecoord: 1..243
IPR017975Tubulin, conserved sitePROSITEPS00227TUBULINcoord: 140..146
IPR013838Beta tubulin, autoregulation binding sitePROSITEPS00228TUBULIN_B_AUTOREGcoord: 1..4
IPR008280Tubulin/FtsZ, C-terminalSUPERFAMILYSSF55307Tubulin C-terminal domain-likecoord: 244..429