SMED30027856

Overview
NameSMED30027856
Smed IDSMED30027856
Length (bp)502
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30027856 (SMED30027856) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30027856 (SMED30027856) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30027856 (SMED30027856) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30027856 (SMED30027856) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30027856

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 15

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30027856h1SMcG0020629 Contig2221GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30027856h1SMcG0020573 Contig2221GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30027856h1SMcG0020513 Contig2221GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30027856h1SMcG0020520 Contig2221GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30027856h1SMcG0020450 Contig2221GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30027856h1SMcG0020518 Contig2221GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30027856h1SMcG0020573 PL08004B1G06smed_ncbi_20240424PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30027856 Contig1674GPL15192PMID:23079596
Forsthoefel et al., 2012
whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30027856 Contig1674GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
prepharyngeal regionSMED30027856h1SMcG0020573 SmedASXL_073723SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30027856h1SMcG0020573 Contig31350uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027856h1SMcG0020573 Contig31350newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30027856h1SMcG0020573 dd_Smed_v4_3945_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30027856h1SMcG0020573 dd_Smed_v4_8471_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30027856h1SMcG0020559 dd_Smed_v4_3945_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30027856 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001170:75610..76294 +2447
Homology
BLAST of SMED30027856 vs. Ensembl Human
Match: ANK3 (ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494])

HSP 1 Score: 78.5666 bits (192), Expect = 5.577e-17
Identity = 34/90 (37.78%), Postives = 56/90 (62.22%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
             G  P+HIA   G ++VA LLL+    +D  A+    P+H AS+ G++ ++KLLLDR    + +T+ G  P+H  +  GH +++++LLDR
Sbjct:  217 SGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDR 306          

HSP 2 Score: 74.7146 bits (182), Expect = 1.294e-15
Identity = 30/84 (35.71%), Postives = 52/84 (61.90%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            P+H+A + G  ++ +LLLD    ID + +    P+H  +R GH +++++LLDR      +TK+G  P+H A+ G HL  +++LL
Sbjct:  254 PLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLL 337          

HSP 3 Score: 70.8626 bits (172), Expect = 3.111e-14
Identity = 30/91 (32.97%), Postives = 54/91 (59.34%), Query Frame = 1
Query:  226 DDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            + +G   +H+A +EG V+V   LL    N+D   K+    +H AS  G +E++K+L+    N   ++++G+ P++ A+   HLE++K LLD
Sbjct:   54 NQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLD 144          

HSP 4 Score: 63.5438 bits (153), Expect = 8.178e-12
Identity = 31/110 (28.18%), Postives = 60/110 (54.55%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G+ E V E L+ E         G+  +HIA   G  +V ++L+ N  N++ +++  + P++ A++  H E++K LLD   +    T+ G+ P+  A   GH +++ +LL+
Sbjct:   68 GHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLE 177          

HSP 5 Score: 63.5438 bits (153), Expect = 1.060e-11
Identity = 31/85 (36.47%), Postives = 49/85 (57.65%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R+     A LLL N+ N D E+K  + P+H A+  G+  +  LLL+R    +   ++   P+H AS  G+  ++K+LLDR
Sbjct:  189 LHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDR 273          

HSP 6 Score: 59.6918 bits (143), Expect = 2.277e-10
Identity = 30/91 (32.97%), Postives = 53/91 (58.24%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +DG  P+ +A ++G   V  LLL+N    D + K +   +H A+R   ++   LLL  + N + E+KSG+ P+H A+  G++ +  +LL+R
Sbjct:  154 EDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNR 240          

HSP 7 Score: 58.151 bits (139), Expect = 7.391e-10
Identity = 27/91 (29.67%), Postives = 50/91 (54.95%), Query Frame = 1
Query:  223 IDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            + +D    +H+A   G   VA++LLD   N + +A   + P+H A +    ++++LLL    + +  T+SG  P+H A+  GH+ I+  L+
Sbjct:  346 VTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLM 436          

HSP 8 Score: 52.373 bits (124), Expect = 6.960e-8
Identity = 24/88 (27.27%), Postives = 49/88 (55.68%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +   ++  +LLL +N  +D         +H A+  GH ++ K+LLD+  N   +  +G+ P+H A     ++++++LL
Sbjct:  316 NGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLL 403          
BLAST of SMED30027856 vs. Ensembl Human
Match: ANK1 (ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492])

HSP 1 Score: 78.1814 bits (191), Expect = 9.378e-17
Identity = 36/88 (40.91%), Postives = 55/88 (62.50%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIA R G V + RLLLD    I+ + K +  P+H A+R GH  I ++LLD     + +TK+G  P+H A+ G HL+ +++LL
Sbjct:  238 NGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLL 325          

HSP 2 Score: 74.7146 bits (182), Expect = 1.323e-15
Identity = 34/86 (39.53%), Postives = 54/86 (62.79%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H+A R G  +VA+ LL N   ++ +AK    P+H A+R GH+ ++KLLL+ N N    T +G  P+H A+  GH+E +  LL++
Sbjct:  440 PLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEK 525          

HSP 3 Score: 68.9366 bits (167), Expect = 1.618e-13
Identity = 32/90 (35.56%), Postives = 52/90 (57.78%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            D   P+H A R G  ++ +LLL+NN N +        P+H A+R GH E +  LL++  +  C TK G+ P+H A+  G + + ++LL+R
Sbjct:  469 DDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLER 558          

HSP 4 Score: 68.9366 bits (167), Expect = 1.633e-13
Identity = 35/93 (37.63%), Postives = 55/93 (59.14%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            V+   G  P+HIA     ++VA+LLL+   +++   +    P+H ASR G+  +++LLLDR    E +TK    P+H A+  GH+ I +ILLD
Sbjct:  201 VLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLD 293          

HSP 5 Score: 68.5514 bits (166), Expect = 1.904e-13
Identity = 33/89 (37.08%), Postives = 53/89 (59.55%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            GH P+HIA REG V+    LL+   +  C  K+ + P+H A++ G   + +LLL+R+ +     K+G  P+H A    +L+I+K+LL R
Sbjct:  503 GHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPR 591          

HSP 6 Score: 65.855 bits (159), Expect = 1.924e-12
Identity = 34/109 (31.19%), Postives = 60/109 (55.05%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+AE V   L  +    + +  G  P+H+  +EG V VA +L+ +   +D   +  + P+H AS  G+ +++K LL    +   +TK G+ P+H A+  GH +I+ +LL
Sbjct:  646 GHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 754          

HSP 7 Score: 65.4698 bits (158), Expect = 2.566e-12
Identity = 30/88 (34.09%), Postives = 53/88 (60.23%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G+ P+HIA ++  V+VAR LL    + + E+ +   P+H A++ GH+E++ LLL +  N     KSG  P+H  +  GH+ +  +L+
Sbjct:  601 NGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLI 688          

HSP 8 Score: 64.3142 bits (155), Expect = 6.630e-12
Identity = 37/99 (37.37%), Postives = 53/99 (53.54%), Query Frame = 1
Query:  214 DKVIDD---DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            D  IDD   D   P+H+A   G   VA++LLD     +  A   + P+H A +  H  +++LLL    + +  T+SG  P+H AS  GHL I+K LL R
Sbjct:  328 DAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQR 426          

HSP 9 Score: 62.3882 bits (150), Expect = 2.872e-11
Identity = 30/86 (34.88%), Postives = 48/86 (55.81%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H A R G V ++ +LLD+   I  + K    PIH A++G H + ++LLL  +   +  T     P+H A+  GH  + K+LLD+
Sbjct:  275 PLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDK 360          

HSP 10 Score: 60.8474 bits (146), Expect = 8.555e-11
Identity = 33/88 (37.50%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  V V  LLL    +ID   +    P+H AS  GH  I+K LL R  +          P+H A+  GH E+ K LL
Sbjct:  370 NGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLL 457          

HSP 11 Score: 56.6102 bits (135), Expect = 2.939e-9
Identity = 28/88 (31.82%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  PIH+A +   +D  RLLL  +  ID        P+H A+  GH  + K+LLD+         +G+ P+H A    H+ ++++LL
Sbjct:  304 NGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLL 391          

HSP 12 Score: 55.8398 bits (133), Expect = 5.788e-9
Identity = 33/109 (30.28%), Postives = 55/109 (50.46%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+ E V   L+ E     +   G  P+H+A + G V VA LLL+ + + +   K    P+H A    + +I+KLLL R  +      +G+ P+H A+    +E+ + LL
Sbjct:  514 GHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLL 622          

HSP 13 Score: 55.0694 bits (131), Expect = 9.687e-9
Identity = 29/99 (29.29%), Postives = 50/99 (50.51%), Query Frame = 1
Query:  202 GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  +D V +  G  P+H+A   G + + + LL    + +    +   P+H A+R GH+E+ K LL        + K    P+H A+  GH  ++K+LL+
Sbjct:  394 GASIDAVTES-GLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLE 491          

HSP 14 Score: 54.6842 bits (130), Expect = 1.378e-8
Identity = 26/87 (29.89%), Postives = 47/87 (54.02%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G  P+H+A +EG  ++  LLL    N +   K    P+H  ++ GH  +  +L+      +  T+ G+ P+H AS  G+++++K LL
Sbjct:  635 GVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLL 721          

HSP 15 Score: 53.1434 bits (126), Expect = 4.450e-8
Identity = 27/111 (24.32%), Postives = 58/111 (52.25%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +GN +   ++L+        + +G   +H+A +EG V +   LL     ++   K+    +H A+  G  E+++ L++   N   +++ G+ P++ A+   HLE++K LL+
Sbjct:   22 SGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLE 132          

HSP 16 Score: 53.1434 bits (126), Expect = 4.941e-8
Identity = 28/85 (32.94%), Postives = 49/85 (57.65%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R      A +LL N+ N D  +K  + P+H A+   +  + +LLL+R  +     ++G  P+H AS  G++ ++++LLDR
Sbjct:  177 LHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDR 261          

HSP 17 Score: 50.447 bits (119), Expect = 3.279e-7
Identity = 24/91 (26.37%), Postives = 51/91 (56.04%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHL---EILKILLD 498
            G+ P+H+A   G + + + LL +  +++ + K  + P+H A++ GH++I+ LLL    +    +  G  P+  A   G++   ++LK++ D
Sbjct:  701 GYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVLKVVTD 791          

HSP 18 Score: 50.447 bits (119), Expect = 4.080e-7
Identity = 25/90 (27.78%), Postives = 46/90 (51.11%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A     +D+ +LLL    +    A   + P+H A++    E+ + LL    +   E+  G  P+H A+  GH E++ +LL +
Sbjct:  568 NGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSK 657          
BLAST of SMED30027856 vs. Ensembl Human
Match: ANK1 (ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492])

HSP 1 Score: 78.1814 bits (191), Expect = 9.378e-17
Identity = 36/88 (40.91%), Postives = 55/88 (62.50%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIA R G V + RLLLD    I+ + K +  P+H A+R GH  I ++LLD     + +TK+G  P+H A+ G HL+ +++LL
Sbjct:  238 NGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLL 325          

HSP 2 Score: 74.7146 bits (182), Expect = 1.323e-15
Identity = 34/86 (39.53%), Postives = 54/86 (62.79%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H+A R G  +VA+ LL N   ++ +AK    P+H A+R GH+ ++KLLL+ N N    T +G  P+H A+  GH+E +  LL++
Sbjct:  440 PLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEK 525          

HSP 3 Score: 68.9366 bits (167), Expect = 1.618e-13
Identity = 32/90 (35.56%), Postives = 52/90 (57.78%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            D   P+H A R G  ++ +LLL+NN N +        P+H A+R GH E +  LL++  +  C TK G+ P+H A+  G + + ++LL+R
Sbjct:  469 DDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLER 558          

HSP 4 Score: 68.9366 bits (167), Expect = 1.633e-13
Identity = 35/93 (37.63%), Postives = 55/93 (59.14%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            V+   G  P+HIA     ++VA+LLL+   +++   +    P+H ASR G+  +++LLLDR    E +TK    P+H A+  GH+ I +ILLD
Sbjct:  201 VLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLD 293          

HSP 5 Score: 68.5514 bits (166), Expect = 1.905e-13
Identity = 33/89 (37.08%), Postives = 53/89 (59.55%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            GH P+HIA REG V+    LL+   +  C  K+ + P+H A++ G   + +LLL+R+ +     K+G  P+H A    +L+I+K+LL R
Sbjct:  503 GHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPR 591          

HSP 6 Score: 65.855 bits (159), Expect = 1.924e-12
Identity = 34/109 (31.19%), Postives = 60/109 (55.05%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+AE V   L  +    + +  G  P+H+  +EG V VA +L+ +   +D   +  + P+H AS  G+ +++K LL    +   +TK G+ P+H A+  GH +I+ +LL
Sbjct:  646 GHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 754          

HSP 7 Score: 65.4698 bits (158), Expect = 2.566e-12
Identity = 30/88 (34.09%), Postives = 53/88 (60.23%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G+ P+HIA ++  V+VAR LL    + + E+ +   P+H A++ GH+E++ LLL +  N     KSG  P+H  +  GH+ +  +L+
Sbjct:  601 NGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLI 688          

HSP 8 Score: 64.3142 bits (155), Expect = 6.630e-12
Identity = 37/99 (37.37%), Postives = 53/99 (53.54%), Query Frame = 1
Query:  214 DKVIDD---DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            D  IDD   D   P+H+A   G   VA++LLD     +  A   + P+H A +  H  +++LLL    + +  T+SG  P+H AS  GHL I+K LL R
Sbjct:  328 DAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQR 426          

HSP 9 Score: 62.3882 bits (150), Expect = 2.872e-11
Identity = 30/86 (34.88%), Postives = 48/86 (55.81%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H A R G V ++ +LLD+   I  + K    PIH A++G H + ++LLL  +   +  T     P+H A+  GH  + K+LLD+
Sbjct:  275 PLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDK 360          

HSP 10 Score: 60.8474 bits (146), Expect = 8.555e-11
Identity = 33/88 (37.50%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  V V  LLL    +ID   +    P+H AS  GH  I+K LL R  +          P+H A+  GH E+ K LL
Sbjct:  370 NGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLL 457          

HSP 11 Score: 56.6102 bits (135), Expect = 2.939e-9
Identity = 28/88 (31.82%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  PIH+A +   +D  RLLL  +  ID        P+H A+  GH  + K+LLD+         +G+ P+H A    H+ ++++LL
Sbjct:  304 NGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLL 391          

HSP 12 Score: 55.8398 bits (133), Expect = 5.788e-9
Identity = 33/109 (30.28%), Postives = 55/109 (50.46%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+ E V   L+ E     +   G  P+H+A + G V VA LLL+ + + +   K    P+H A    + +I+KLLL R  +      +G+ P+H A+    +E+ + LL
Sbjct:  514 GHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLL 622          

HSP 13 Score: 55.0694 bits (131), Expect = 9.687e-9
Identity = 29/99 (29.29%), Postives = 50/99 (50.51%), Query Frame = 1
Query:  202 GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  +D V +  G  P+H+A   G + + + LL    + +    +   P+H A+R GH+E+ K LL        + K    P+H A+  GH  ++K+LL+
Sbjct:  394 GASIDAVTES-GLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLE 491          

HSP 14 Score: 54.6842 bits (130), Expect = 1.378e-8
Identity = 26/87 (29.89%), Postives = 47/87 (54.02%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G  P+H+A +EG  ++  LLL    N +   K    P+H  ++ GH  +  +L+      +  T+ G+ P+H AS  G+++++K LL
Sbjct:  635 GVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLL 721          

HSP 15 Score: 53.1434 bits (126), Expect = 4.450e-8
Identity = 27/111 (24.32%), Postives = 58/111 (52.25%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +GN +   ++L+        + +G   +H+A +EG V +   LL     ++   K+    +H A+  G  E+++ L++   N   +++ G+ P++ A+   HLE++K LL+
Sbjct:   22 SGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLE 132          

HSP 16 Score: 53.1434 bits (126), Expect = 4.941e-8
Identity = 28/85 (32.94%), Postives = 49/85 (57.65%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R      A +LL N+ N D  +K  + P+H A+   +  + +LLL+R  +     ++G  P+H AS  G++ ++++LLDR
Sbjct:  177 LHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDR 261          

HSP 17 Score: 50.447 bits (119), Expect = 3.279e-7
Identity = 24/91 (26.37%), Postives = 51/91 (56.04%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHL---EILKILLD 498
            G+ P+H+A   G + + + LL +  +++ + K  + P+H A++ GH++I+ LLL    +    +  G  P+  A   G++   ++LK++ D
Sbjct:  701 GYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVLKVVTD 791          

HSP 18 Score: 50.447 bits (119), Expect = 4.080e-7
Identity = 25/90 (27.78%), Postives = 46/90 (51.11%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A     +D+ +LLL    +    A   + P+H A++    E+ + LL    +   E+  G  P+H A+  GH E++ +LL +
Sbjct:  568 NGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSK 657          
BLAST of SMED30027856 vs. Ensembl Human
Match: ANK1 (ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492])

HSP 1 Score: 78.1814 bits (191), Expect = 9.383e-17
Identity = 36/88 (40.91%), Postives = 55/88 (62.50%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIA R G V + RLLLD    I+ + K +  P+H A+R GH  I ++LLD     + +TK+G  P+H A+ G HL+ +++LL
Sbjct:  271 NGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLL 358          

HSP 2 Score: 74.7146 bits (182), Expect = 1.324e-15
Identity = 34/86 (39.53%), Postives = 54/86 (62.79%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H+A R G  +VA+ LL N   ++ +AK    P+H A+R GH+ ++KLLL+ N N    T +G  P+H A+  GH+E +  LL++
Sbjct:  473 PLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEK 558          

HSP 3 Score: 68.9366 bits (167), Expect = 1.618e-13
Identity = 32/90 (35.56%), Postives = 52/90 (57.78%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            D   P+H A R G  ++ +LLL+NN N +        P+H A+R GH E +  LL++  +  C TK G+ P+H A+  G + + ++LL+R
Sbjct:  502 DDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLER 591          

HSP 4 Score: 68.9366 bits (167), Expect = 1.634e-13
Identity = 35/93 (37.63%), Postives = 55/93 (59.14%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            V+   G  P+HIA     ++VA+LLL+   +++   +    P+H ASR G+  +++LLLDR    E +TK    P+H A+  GH+ I +ILLD
Sbjct:  234 VLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLD 326          

HSP 5 Score: 68.5514 bits (166), Expect = 1.905e-13
Identity = 33/89 (37.08%), Postives = 53/89 (59.55%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            GH P+HIA REG V+    LL+   +  C  K+ + P+H A++ G   + +LLL+R+ +     K+G  P+H A    +L+I+K+LL R
Sbjct:  536 GHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPR 624          

HSP 6 Score: 65.855 bits (159), Expect = 1.925e-12
Identity = 34/109 (31.19%), Postives = 60/109 (55.05%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+AE V   L  +    + +  G  P+H+  +EG V VA +L+ +   +D   +  + P+H AS  G+ +++K LL    +   +TK G+ P+H A+  GH +I+ +LL
Sbjct:  679 GHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 787          

HSP 7 Score: 65.4698 bits (158), Expect = 2.567e-12
Identity = 30/88 (34.09%), Postives = 53/88 (60.23%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G+ P+HIA ++  V+VAR LL    + + E+ +   P+H A++ GH+E++ LLL +  N     KSG  P+H  +  GH+ +  +L+
Sbjct:  634 NGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLI 721          

HSP 8 Score: 64.3142 bits (155), Expect = 6.633e-12
Identity = 37/99 (37.37%), Postives = 53/99 (53.54%), Query Frame = 1
Query:  214 DKVIDD---DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            D  IDD   D   P+H+A   G   VA++LLD     +  A   + P+H A +  H  +++LLL    + +  T+SG  P+H AS  GHL I+K LL R
Sbjct:  361 DAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQR 459          

HSP 9 Score: 62.3882 bits (150), Expect = 2.874e-11
Identity = 30/86 (34.88%), Postives = 48/86 (55.81%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H A R G V ++ +LLD+   I  + K    PIH A++G H + ++LLL  +   +  T     P+H A+  GH  + K+LLD+
Sbjct:  308 PLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDK 393          

HSP 10 Score: 60.8474 bits (146), Expect = 8.559e-11
Identity = 33/88 (37.50%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  V V  LLL    +ID   +    P+H AS  GH  I+K LL R  +          P+H A+  GH E+ K LL
Sbjct:  403 NGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLL 490          

HSP 11 Score: 56.6102 bits (135), Expect = 2.941e-9
Identity = 28/88 (31.82%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  PIH+A +   +D  RLLL  +  ID        P+H A+  GH  + K+LLD+         +G+ P+H A    H+ ++++LL
Sbjct:  337 NGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLL 424          

HSP 12 Score: 55.8398 bits (133), Expect = 5.791e-9
Identity = 33/109 (30.28%), Postives = 55/109 (50.46%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+ E V   L+ E     +   G  P+H+A + G V VA LLL+ + + +   K    P+H A    + +I+KLLL R  +      +G+ P+H A+    +E+ + LL
Sbjct:  547 GHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLL 655          

HSP 13 Score: 55.0694 bits (131), Expect = 9.692e-9
Identity = 29/99 (29.29%), Postives = 50/99 (50.51%), Query Frame = 1
Query:  202 GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  +D V +  G  P+H+A   G + + + LL    + +    +   P+H A+R GH+E+ K LL        + K    P+H A+  GH  ++K+LL+
Sbjct:  427 GASIDAVTES-GLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLE 524          

HSP 14 Score: 54.6842 bits (130), Expect = 1.379e-8
Identity = 26/87 (29.89%), Postives = 47/87 (54.02%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G  P+H+A +EG  ++  LLL    N +   K    P+H  ++ GH  +  +L+      +  T+ G+ P+H AS  G+++++K LL
Sbjct:  668 GVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLL 754          

HSP 15 Score: 53.1434 bits (126), Expect = 4.452e-8
Identity = 27/111 (24.32%), Postives = 58/111 (52.25%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +GN +   ++L+        + +G   +H+A +EG V +   LL     ++   K+    +H A+  G  E+++ L++   N   +++ G+ P++ A+   HLE++K LL+
Sbjct:   55 SGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLE 165          

HSP 16 Score: 53.1434 bits (126), Expect = 4.943e-8
Identity = 28/85 (32.94%), Postives = 49/85 (57.65%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R      A +LL N+ N D  +K  + P+H A+   +  + +LLL+R  +     ++G  P+H AS  G++ ++++LLDR
Sbjct:  210 LHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDR 294          

HSP 17 Score: 50.447 bits (119), Expect = 3.281e-7
Identity = 24/91 (26.37%), Postives = 51/91 (56.04%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHL---EILKILLD 498
            G+ P+H+A   G + + + LL +  +++ + K  + P+H A++ GH++I+ LLL    +    +  G  P+  A   G++   ++LK++ D
Sbjct:  734 GYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVLKVVTD 824          

HSP 18 Score: 50.447 bits (119), Expect = 4.082e-7
Identity = 25/90 (27.78%), Postives = 46/90 (51.11%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A     +D+ +LLL    +    A   + P+H A++    E+ + LL    +   E+  G  P+H A+  GH E++ +LL +
Sbjct:  601 NGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSK 690          
BLAST of SMED30027856 vs. Ensembl Human
Match: ANK2 (ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493])

HSP 1 Score: 76.6406 bits (187), Expect = 3.566e-16
Identity = 35/91 (38.46%), Postives = 58/91 (63.74%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            + G  P+HIA   G V+VA LLL+    +D  A+    P+H AS+ G++ ++KLLLDR    + +T+ G  P+H A+  GH +++++LL+R
Sbjct:  210 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLER 300          

HSP 2 Score: 74.3294 bits (181), Expect = 2.115e-15
Identity = 33/88 (37.50%), Postives = 54/88 (61.36%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A + G  ++ +LLLD    ID + +    P+H A+R GH ++++LLL+R       TK+G  P+H A+ G H+E +K LL
Sbjct:  244 NGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLL 331          

HSP 3 Score: 66.6254 bits (161), Expect = 1.050e-12
Identity = 31/88 (35.23%), Postives = 53/88 (60.23%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G   +H+A R G V+V R LL N   +D  A+ +  P+H ASR G +EI++LLL    + +  T +G+ P+H ++  G +++  +LL+
Sbjct:  443 GETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLE 530          

HSP 4 Score: 66.2402 bits (160), Expect = 1.454e-12
Identity = 32/110 (29.09%), Postives = 62/110 (56.36%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            GN + V E LKG       + +G   +H+A +EG V + + LL    ++D   K+    +H AS  G +E++K+L+    N   ++++G+ P++ A+   H++++K LL+
Sbjct:   21 GNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLE 130          

HSP 5 Score: 61.6178 bits (148), Expect = 5.448e-11
Identity = 36/112 (32.14%), Postives = 59/112 (52.68%), Query Frame = 1
Query:  169 GNAEGVRENLK-GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  E VR  L+ G  VD    ++   P+HIA R G  ++ +LLL +  + D      + P+H ++R G  ++  +LL+        TK G+ P+H A+  G L++ K+LL R
Sbjct:  454 GQVEVVRCLLRNGALVDARAREE-QTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQR 564          

HSP 6 Score: 60.077 bits (144), Expect = 1.561e-10
Identity = 30/90 (33.33%), Postives = 53/90 (58.89%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+ P+HI+ REG VDVA +LL+         K+ + P+H A++ G  ++ KLLL R    +   K+G  P+H A+   + ++  +LL++
Sbjct:  508 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEK 597          

HSP 7 Score: 59.3066 bits (142), Expect = 3.554e-10
Identity = 28/87 (32.18%), Postives = 49/87 (56.32%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            +G+ P+HIA ++  + +A  LL+     +   K+   P+H AS+ GH++++ LLLD+  N    TKSG   +H A+    + +  IL
Sbjct:  607 NGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADIL 693          

HSP 8 Score: 56.6102 bits (135), Expect = 3.022e-9
Identity = 27/88 (30.68%), Postives = 47/88 (53.41%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A + G +DVA+LLL      D   K    P+H A+   + ++  LLL++  +     K+G+ P+H A+    ++I   LL+
Sbjct:  542 GFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLN 629          

HSP 9 Score: 55.0694 bits (131), Expect = 1.045e-8
Identity = 29/90 (32.22%), Postives = 46/90 (51.11%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A       VA LLL+   +    AK  + P+H A++    +I   LL+        TK G  P+H AS  GH +++ +LLD+
Sbjct:  574 NGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDK 663          

HSP 10 Score: 54.6842 bits (130), Expect = 1.128e-8
Identity = 26/83 (31.33%), Postives = 47/83 (56.63%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A +E  V+VA +L  +  + D   K  + P+  A   G+ +++  LL +  N   +TK+G+ P+H A+  GH  I+ +LL
Sbjct:  678 LHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLL 760          

HSP 11 Score: 54.6842 bits (130), Expect = 1.252e-8
Identity = 31/93 (33.33%), Postives = 50/93 (53.76%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAK--------RKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R+     A LLL N+ N D ++K          + P+H A+  G+  +  LLL+R    +   ++G  P+H AS  G+  ++K+LLDR
Sbjct:  175 LHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDR 267          

HSP 12 Score: 54.299 bits (129), Expect = 1.605e-8
Identity = 27/83 (32.53%), Postives = 46/83 (55.42%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A   G   V +LLLD   N +  A   + P+H A +    ++++LL+    + +  T+SG  P+H A+  GHL I+ +LL
Sbjct:  348 LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL 430          

HSP 13 Score: 53.1434 bits (126), Expect = 4.364e-8
Identity = 28/100 (28.00%), Postives = 54/100 (54.00%), Query Frame = 1
Query:  199 KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +G  VD      G+  +HIA   G  +V ++L+    NI+ +++  + P++ A++  H +++K LL+   N    T+ G+ P+  A   GH + + ILL+
Sbjct:   65 RGSSVDSATKK-GNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLE 163          

HSP 14 Score: 51.2174 bits (121), Expect = 1.944e-7
Identity = 27/88 (30.68%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LL+    +I    +    PIH A+  GH  I+ LLL    + +     G   +H A+  G +E+++ LL
Sbjct:  376 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLL 463          

HSP 15 Score: 48.521 bits (114), Expect = 1.679e-6
Identity = 23/86 (26.74%), Postives = 43/86 (50.00%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+ +AC  G V +   LL    N++ + K  + P+H A++ GH+ I+ +LL         T +G   +  A   G++ ++  L
Sbjct:  707 GYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 792          
BLAST of SMED30027856 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 72.0182 bits (175), Expect = 4.869e-15
Identity = 33/86 (38.37%), Postives = 52/86 (60.47%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+HIA R G  D+  LLL    N +   +  + P+H A++ G  E+  +LLD N +    TK G+ P+H AS  G+LE++++LL+R
Sbjct:  494 PLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLER 579          

HSP 2 Score: 69.707 bits (169), Expect = 3.172e-14
Identity = 33/88 (37.50%), Postives = 50/88 (56.82%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A R    DV R+L+ N   +D +A+    P+H ASR G+++I+ LLL    N    T+  + P+H A+  G  E+  ILLD
Sbjct:  458 GETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLD 545          

HSP 3 Score: 63.1586 bits (152), Expect = 5.641e-12
Identity = 29/84 (34.52%), Postives = 48/84 (57.14%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            P+H+A + G  ++A LLL     ID   K    P+H A+R GH +++ LL+ +      +TK+G  P+H A+ G H++  + LL
Sbjct:  263 PLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLL 346          

HSP 4 Score: 63.1586 bits (152), Expect = 6.208e-12
Identity = 31/100 (31.00%), Postives = 53/100 (53.00%), Query Frame = 1
Query:  196 LKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            L+ E    V    G  P+HIA   G  +V +LLL+   N++ +A+    P+H A++ G + +  LLL R    +  TK    P+H A+  GH +++ +L+
Sbjct:  214 LQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLV 313          

HSP 5 Score: 62.003 bits (149), Expect = 1.311e-11
Identity = 31/89 (34.83%), Postives = 56/89 (62.92%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            D + P+HIA +EG  +VA +LLD+N +     K+ + P+H AS+ G+ E+++LLL+R    + E K+   P+H A+   + ++  +LL+
Sbjct:  523 DNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLE 611          

HSP 6 Score: 59.6918 bits (143), Expect = 8.472e-11
Identity = 28/87 (32.18%), Postives = 49/87 (56.32%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G  P+H+A + G ++V RLLL+    +D E K +  P+H A+   + ++  LLL+   + +   K+G+ P+H A+    +EI   LL
Sbjct:  557 GFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLL 643          

HSP 7 Score: 55.8398 bits (133), Expect = 1.819e-9
Identity = 30/110 (27.27%), Postives = 58/110 (52.73%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G++E VRE +K +         G+  +HIA   G   +  +L++N  N++ ++   + P++ A++  H E++K LL    N    T+ G+ P+  A   GH  ++ +LL+
Sbjct:   77 GHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLE 186          

HSP 8 Score: 55.0694 bits (131), Expect = 3.547e-9
Identity = 29/86 (33.72%), Postives = 48/86 (55.81%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H+A   G V VA+LLLD + + +  A   + P+H A +    ++++LLL      E  T+SG  P+H A+  G + I+  LL +
Sbjct:  362 PLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQ 447          

HSP 9 Score: 55.0694 bits (131), Expect = 3.615e-9
Identity = 30/109 (27.52%), Postives = 56/109 (51.38%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+ E V E L+        + +G   +H+A +EG  +V R L+     +D   ++    +H AS  G S I+ +L++   N   ++ +G+ P++ A+   H E++K LL
Sbjct:   44 GDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLL 152          

HSP 10 Score: 54.6842 bits (130), Expect = 4.292e-9
Identity = 28/88 (31.82%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +   VD AR LL +   +D        P+H A+  GH  + KLLLDR+ +      +G+ P+H A     ++++++LL
Sbjct:  325 NGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLL 412          

HSP 11 Score: 54.299 bits (129), Expect = 6.285e-9
Identity = 27/85 (31.76%), Postives = 44/85 (51.76%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA ++     A LLL N  N D  +K  + P+H A+  GH  + +LLL++  N   + +    P+H A+  G   +  +LL R
Sbjct:  198 LHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSR 282          

HSP 12 Score: 53.9138 bits (128), Expect = 8.207e-9
Identity = 35/111 (31.53%), Postives = 58/111 (52.25%), Query Frame = 1
Query:  169 GNAEGVRENL-KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            GN E VR  L +G  VD +   +   P+H+A       VA LLL+N  +    AK  + P+H A++    EI   LL    +   ++++G+ P+H ++  GH EI  +L++
Sbjct:  568 GNLEVVRLLLERGTPVD-IEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIE 677          

HSP 13 Score: 53.1434 bits (126), Expect = 1.481e-8
Identity = 21/86 (24.42%), Postives = 51/86 (59.30%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+H+AC  G +++ + L++N  ++  + +  + P+H A++ GH+  ++ LL+   +   +T +G  P+  A   G++ +++ L
Sbjct:  722 GYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 14 Score: 53.1434 bits (126), Expect = 1.742e-8
Identity = 26/88 (29.55%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LLL     I+   +    P+H A+  G   I+  LL +  N + ET  G  P+H A+     +++++L+
Sbjct:  391 NGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI 478          

HSP 15 Score: 50.8322 bits (120), Expect = 9.651e-8
Identity = 21/88 (23.86%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H++ +EG  +++ LL++N  ++  +A      +H  ++  H  + ++L +       +T +G+ P+H A   G L ++K L++
Sbjct:  656 GFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVE 743          

HSP 16 Score: 50.447 bits (119), Expect = 1.480e-7
Identity = 23/90 (25.56%), Postives = 49/90 (54.44%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            ++G   +H+  +E  V VA++L +N   I+ +    + P+H A   G   ++K L++   +   +T++ + P+H A+  GH   ++ LL+
Sbjct:  687 NNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLE 776          

HSP 17 Score: 49.2914 bits (116), Expect = 3.348e-7
Identity = 22/94 (23.40%), Postives = 51/94 (54.26%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            K    +G+ P+HIA ++  +++A  LL    + + +++  + P+H +++ GH EI  LL++   +   +  +G   +H  +   H+ + +IL +
Sbjct:  617 KAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYN 710          

HSP 18 Score: 48.1358 bits (113), Expect = 8.643e-7
Identity = 27/91 (29.67%), Postives = 50/91 (54.95%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +DG  P+ +A ++G   V  +LL+N    D + K +   +H A++   +    LLL    N +  +KSG+ P+H A+  GH  + ++LL++
Sbjct:  163 EDGFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEK 249          
BLAST of SMED30027856 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 72.0182 bits (175), Expect = 4.869e-15
Identity = 33/86 (38.37%), Postives = 52/86 (60.47%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+HIA R G  D+  LLL    N +   +  + P+H A++ G  E+  +LLD N +    TK G+ P+H AS  G+LE++++LL+R
Sbjct:  494 PLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLER 579          

HSP 2 Score: 69.707 bits (169), Expect = 3.172e-14
Identity = 33/88 (37.50%), Postives = 50/88 (56.82%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A R    DV R+L+ N   +D +A+    P+H ASR G+++I+ LLL    N    T+  + P+H A+  G  E+  ILLD
Sbjct:  458 GETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLD 545          

HSP 3 Score: 63.1586 bits (152), Expect = 5.641e-12
Identity = 29/84 (34.52%), Postives = 48/84 (57.14%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            P+H+A + G  ++A LLL     ID   K    P+H A+R GH +++ LL+ +      +TK+G  P+H A+ G H++  + LL
Sbjct:  263 PLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLL 346          

HSP 4 Score: 63.1586 bits (152), Expect = 6.208e-12
Identity = 31/100 (31.00%), Postives = 53/100 (53.00%), Query Frame = 1
Query:  196 LKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            L+ E    V    G  P+HIA   G  +V +LLL+   N++ +A+    P+H A++ G + +  LLL R    +  TK    P+H A+  GH +++ +L+
Sbjct:  214 LQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLV 313          

HSP 5 Score: 62.003 bits (149), Expect = 1.311e-11
Identity = 31/89 (34.83%), Postives = 56/89 (62.92%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            D + P+HIA +EG  +VA +LLD+N +     K+ + P+H AS+ G+ E+++LLL+R    + E K+   P+H A+   + ++  +LL+
Sbjct:  523 DNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLE 611          

HSP 6 Score: 59.6918 bits (143), Expect = 8.472e-11
Identity = 28/87 (32.18%), Postives = 49/87 (56.32%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G  P+H+A + G ++V RLLL+    +D E K +  P+H A+   + ++  LLL+   + +   K+G+ P+H A+    +EI   LL
Sbjct:  557 GFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLL 643          

HSP 7 Score: 55.8398 bits (133), Expect = 1.819e-9
Identity = 30/110 (27.27%), Postives = 58/110 (52.73%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G++E VRE +K +         G+  +HIA   G   +  +L++N  N++ ++   + P++ A++  H E++K LL    N    T+ G+ P+  A   GH  ++ +LL+
Sbjct:   77 GHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLE 186          

HSP 8 Score: 55.0694 bits (131), Expect = 3.547e-9
Identity = 29/86 (33.72%), Postives = 48/86 (55.81%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H+A   G V VA+LLLD + + +  A   + P+H A +    ++++LLL      E  T+SG  P+H A+  G + I+  LL +
Sbjct:  362 PLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQ 447          

HSP 9 Score: 55.0694 bits (131), Expect = 3.615e-9
Identity = 30/109 (27.52%), Postives = 56/109 (51.38%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+ E V E L+        + +G   +H+A +EG  +V R L+     +D   ++    +H AS  G S I+ +L++   N   ++ +G+ P++ A+   H E++K LL
Sbjct:   44 GDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLL 152          

HSP 10 Score: 54.6842 bits (130), Expect = 4.292e-9
Identity = 28/88 (31.82%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +   VD AR LL +   +D        P+H A+  GH  + KLLLDR+ +      +G+ P+H A     ++++++LL
Sbjct:  325 NGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLL 412          

HSP 11 Score: 54.299 bits (129), Expect = 6.285e-9
Identity = 27/85 (31.76%), Postives = 44/85 (51.76%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA ++     A LLL N  N D  +K  + P+H A+  GH  + +LLL++  N   + +    P+H A+  G   +  +LL R
Sbjct:  198 LHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSR 282          

HSP 12 Score: 53.9138 bits (128), Expect = 8.207e-9
Identity = 35/111 (31.53%), Postives = 58/111 (52.25%), Query Frame = 1
Query:  169 GNAEGVRENL-KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            GN E VR  L +G  VD +   +   P+H+A       VA LLL+N  +    AK  + P+H A++    EI   LL    +   ++++G+ P+H ++  GH EI  +L++
Sbjct:  568 GNLEVVRLLLERGTPVD-IEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIE 677          

HSP 13 Score: 53.1434 bits (126), Expect = 1.481e-8
Identity = 21/86 (24.42%), Postives = 51/86 (59.30%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+H+AC  G +++ + L++N  ++  + +  + P+H A++ GH+  ++ LL+   +   +T +G  P+  A   G++ +++ L
Sbjct:  722 GYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 14 Score: 53.1434 bits (126), Expect = 1.742e-8
Identity = 26/88 (29.55%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LLL     I+   +    P+H A+  G   I+  LL +  N + ET  G  P+H A+     +++++L+
Sbjct:  391 NGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI 478          

HSP 15 Score: 50.8322 bits (120), Expect = 9.651e-8
Identity = 21/88 (23.86%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H++ +EG  +++ LL++N  ++  +A      +H  ++  H  + ++L +       +T +G+ P+H A   G L ++K L++
Sbjct:  656 GFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVE 743          

HSP 16 Score: 50.447 bits (119), Expect = 1.480e-7
Identity = 23/90 (25.56%), Postives = 49/90 (54.44%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            ++G   +H+  +E  V VA++L +N   I+ +    + P+H A   G   ++K L++   +   +T++ + P+H A+  GH   ++ LL+
Sbjct:  687 NNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLE 776          

HSP 17 Score: 49.2914 bits (116), Expect = 3.348e-7
Identity = 22/94 (23.40%), Postives = 51/94 (54.26%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            K    +G+ P+HIA ++  +++A  LL    + + +++  + P+H +++ GH EI  LL++   +   +  +G   +H  +   H+ + +IL +
Sbjct:  617 KAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYN 710          

HSP 18 Score: 48.1358 bits (113), Expect = 8.643e-7
Identity = 27/91 (29.67%), Postives = 50/91 (54.95%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +DG  P+ +A ++G   V  +LL+N    D + K +   +H A++   +    LLL    N +  +KSG+ P+H A+  GH  + ++LL++
Sbjct:  163 EDGFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEK 249          
BLAST of SMED30027856 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 72.0182 bits (175), Expect = 4.873e-15
Identity = 33/86 (38.37%), Postives = 52/86 (60.47%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+HIA R G  D+  LLL    N +   +  + P+H A++ G  E+  +LLD N +    TK G+ P+H AS  G+LE++++LL+R
Sbjct:  494 PLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLER 579          

HSP 2 Score: 69.707 bits (169), Expect = 3.174e-14
Identity = 33/88 (37.50%), Postives = 50/88 (56.82%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A R    DV R+L+ N   +D +A+    P+H ASR G+++I+ LLL    N    T+  + P+H A+  G  E+  ILLD
Sbjct:  458 GETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLD 545          

HSP 3 Score: 63.1586 bits (152), Expect = 5.646e-12
Identity = 29/84 (34.52%), Postives = 48/84 (57.14%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            P+H+A + G  ++A LLL     ID   K    P+H A+R GH +++ LL+ +      +TK+G  P+H A+ G H++  + LL
Sbjct:  263 PLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLL 346          

HSP 4 Score: 63.1586 bits (152), Expect = 6.213e-12
Identity = 31/100 (31.00%), Postives = 53/100 (53.00%), Query Frame = 1
Query:  196 LKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            L+ E    V    G  P+HIA   G  +V +LLL+   N++ +A+    P+H A++ G + +  LLL R    +  TK    P+H A+  GH +++ +L+
Sbjct:  214 LQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLV 313          

HSP 5 Score: 62.003 bits (149), Expect = 1.312e-11
Identity = 31/89 (34.83%), Postives = 56/89 (62.92%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            D + P+HIA +EG  +VA +LLD+N +     K+ + P+H AS+ G+ E+++LLL+R    + E K+   P+H A+   + ++  +LL+
Sbjct:  523 DNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLE 611          

HSP 6 Score: 59.6918 bits (143), Expect = 8.479e-11
Identity = 28/87 (32.18%), Postives = 49/87 (56.32%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G  P+H+A + G ++V RLLL+    +D E K +  P+H A+   + ++  LLL+   + +   K+G+ P+H A+    +EI   LL
Sbjct:  557 GFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLL 643          

HSP 7 Score: 55.8398 bits (133), Expect = 1.821e-9
Identity = 30/110 (27.27%), Postives = 58/110 (52.73%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G++E VRE +K +         G+  +HIA   G   +  +L++N  N++ ++   + P++ A++  H E++K LL    N    T+ G+ P+  A   GH  ++ +LL+
Sbjct:   77 GHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLE 186          

HSP 8 Score: 55.0694 bits (131), Expect = 3.550e-9
Identity = 29/86 (33.72%), Postives = 48/86 (55.81%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H+A   G V VA+LLLD + + +  A   + P+H A +    ++++LLL      E  T+SG  P+H A+  G + I+  LL +
Sbjct:  362 PLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQ 447          

HSP 9 Score: 55.0694 bits (131), Expect = 3.618e-9
Identity = 30/109 (27.52%), Postives = 56/109 (51.38%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+ E V E L+        + +G   +H+A +EG  +V R L+     +D   ++    +H AS  G S I+ +L++   N   ++ +G+ P++ A+   H E++K LL
Sbjct:   44 GDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLL 152          

HSP 10 Score: 54.6842 bits (130), Expect = 4.296e-9
Identity = 28/88 (31.82%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +   VD AR LL +   +D        P+H A+  GH  + KLLLDR+ +      +G+ P+H A     ++++++LL
Sbjct:  325 NGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLL 412          

HSP 11 Score: 54.299 bits (129), Expect = 6.290e-9
Identity = 27/85 (31.76%), Postives = 44/85 (51.76%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA ++     A LLL N  N D  +K  + P+H A+  GH  + +LLL++  N   + +    P+H A+  G   +  +LL R
Sbjct:  198 LHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSR 282          

HSP 12 Score: 53.9138 bits (128), Expect = 8.213e-9
Identity = 35/111 (31.53%), Postives = 58/111 (52.25%), Query Frame = 1
Query:  169 GNAEGVRENL-KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            GN E VR  L +G  VD +   +   P+H+A       VA LLL+N  +    AK  + P+H A++    EI   LL    +   ++++G+ P+H ++  GH EI  +L++
Sbjct:  568 GNLEVVRLLLERGTPVD-IEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIE 677          

HSP 13 Score: 53.1434 bits (126), Expect = 1.483e-8
Identity = 21/86 (24.42%), Postives = 51/86 (59.30%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+H+AC  G +++ + L++N  ++  + +  + P+H A++ GH+  ++ LL+   +   +T +G  P+  A   G++ +++ L
Sbjct:  722 GYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 14 Score: 53.1434 bits (126), Expect = 1.743e-8
Identity = 26/88 (29.55%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LLL     I+   +    P+H A+  G   I+  LL +  N + ET  G  P+H A+     +++++L+
Sbjct:  391 NGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI 478          

HSP 15 Score: 50.8322 bits (120), Expect = 9.659e-8
Identity = 21/88 (23.86%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H++ +EG  +++ LL++N  ++  +A      +H  ++  H  + ++L +       +T +G+ P+H A   G L ++K L++
Sbjct:  656 GFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVE 743          

HSP 16 Score: 50.447 bits (119), Expect = 1.481e-7
Identity = 23/90 (25.56%), Postives = 49/90 (54.44%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            ++G   +H+  +E  V VA++L +N   I+ +    + P+H A   G   ++K L++   +   +T++ + P+H A+  GH   ++ LL+
Sbjct:  687 NNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLE 776          

HSP 17 Score: 49.2914 bits (116), Expect = 3.351e-7
Identity = 22/94 (23.40%), Postives = 51/94 (54.26%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            K    +G+ P+HIA ++  +++A  LL    + + +++  + P+H +++ GH EI  LL++   +   +  +G   +H  +   H+ + +IL +
Sbjct:  617 KAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYN 710          

HSP 18 Score: 48.1358 bits (113), Expect = 8.649e-7
Identity = 27/91 (29.67%), Postives = 50/91 (54.95%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +DG  P+ +A ++G   V  +LL+N    D + K +   +H A++   +    LLL    N +  +KSG+ P+H A+  GH  + ++LL++
Sbjct:  163 EDGFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEK 249          
BLAST of SMED30027856 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 72.0182 bits (175), Expect = 4.873e-15
Identity = 33/86 (38.37%), Postives = 52/86 (60.47%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+HIA R G  D+  LLL    N +   +  + P+H A++ G  E+  +LLD N +    TK G+ P+H AS  G+LE++++LL+R
Sbjct:  494 PLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLER 579          

HSP 2 Score: 69.707 bits (169), Expect = 3.174e-14
Identity = 33/88 (37.50%), Postives = 50/88 (56.82%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A R    DV R+L+ N   +D +A+    P+H ASR G+++I+ LLL    N    T+  + P+H A+  G  E+  ILLD
Sbjct:  458 GETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLD 545          

HSP 3 Score: 63.1586 bits (152), Expect = 5.646e-12
Identity = 29/84 (34.52%), Postives = 48/84 (57.14%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            P+H+A + G  ++A LLL     ID   K    P+H A+R GH +++ LL+ +      +TK+G  P+H A+ G H++  + LL
Sbjct:  263 PLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLL 346          

HSP 4 Score: 63.1586 bits (152), Expect = 6.213e-12
Identity = 31/100 (31.00%), Postives = 53/100 (53.00%), Query Frame = 1
Query:  196 LKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            L+ E    V    G  P+HIA   G  +V +LLL+   N++ +A+    P+H A++ G + +  LLL R    +  TK    P+H A+  GH +++ +L+
Sbjct:  214 LQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLV 313          

HSP 5 Score: 62.003 bits (149), Expect = 1.312e-11
Identity = 31/89 (34.83%), Postives = 56/89 (62.92%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            D + P+HIA +EG  +VA +LLD+N +     K+ + P+H AS+ G+ E+++LLL+R    + E K+   P+H A+   + ++  +LL+
Sbjct:  523 DNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLE 611          

HSP 6 Score: 59.6918 bits (143), Expect = 8.479e-11
Identity = 28/87 (32.18%), Postives = 49/87 (56.32%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G  P+H+A + G ++V RLLL+    +D E K +  P+H A+   + ++  LLL+   + +   K+G+ P+H A+    +EI   LL
Sbjct:  557 GFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLL 643          

HSP 7 Score: 55.8398 bits (133), Expect = 1.821e-9
Identity = 30/110 (27.27%), Postives = 58/110 (52.73%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G++E VRE +K +         G+  +HIA   G   +  +L++N  N++ ++   + P++ A++  H E++K LL    N    T+ G+ P+  A   GH  ++ +LL+
Sbjct:   77 GHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLE 186          

HSP 8 Score: 55.0694 bits (131), Expect = 3.550e-9
Identity = 29/86 (33.72%), Postives = 48/86 (55.81%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H+A   G V VA+LLLD + + +  A   + P+H A +    ++++LLL      E  T+SG  P+H A+  G + I+  LL +
Sbjct:  362 PLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQ 447          

HSP 9 Score: 55.0694 bits (131), Expect = 3.618e-9
Identity = 30/109 (27.52%), Postives = 56/109 (51.38%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+ E V E L+        + +G   +H+A +EG  +V R L+     +D   ++    +H AS  G S I+ +L++   N   ++ +G+ P++ A+   H E++K LL
Sbjct:   44 GDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLL 152          

HSP 10 Score: 54.6842 bits (130), Expect = 4.296e-9
Identity = 28/88 (31.82%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +   VD AR LL +   +D        P+H A+  GH  + KLLLDR+ +      +G+ P+H A     ++++++LL
Sbjct:  325 NGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLL 412          

HSP 11 Score: 54.299 bits (129), Expect = 6.290e-9
Identity = 27/85 (31.76%), Postives = 44/85 (51.76%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA ++     A LLL N  N D  +K  + P+H A+  GH  + +LLL++  N   + +    P+H A+  G   +  +LL R
Sbjct:  198 LHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSR 282          

HSP 12 Score: 53.9138 bits (128), Expect = 8.213e-9
Identity = 35/111 (31.53%), Postives = 58/111 (52.25%), Query Frame = 1
Query:  169 GNAEGVRENL-KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            GN E VR  L +G  VD +   +   P+H+A       VA LLL+N  +    AK  + P+H A++    EI   LL    +   ++++G+ P+H ++  GH EI  +L++
Sbjct:  568 GNLEVVRLLLERGTPVD-IEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIE 677          

HSP 13 Score: 53.1434 bits (126), Expect = 1.483e-8
Identity = 21/86 (24.42%), Postives = 51/86 (59.30%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+H+AC  G +++ + L++N  ++  + +  + P+H A++ GH+  ++ LL+   +   +T +G  P+  A   G++ +++ L
Sbjct:  722 GYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 14 Score: 53.1434 bits (126), Expect = 1.743e-8
Identity = 26/88 (29.55%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LLL     I+   +    P+H A+  G   I+  LL +  N + ET  G  P+H A+     +++++L+
Sbjct:  391 NGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI 478          

HSP 15 Score: 50.8322 bits (120), Expect = 9.659e-8
Identity = 21/88 (23.86%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H++ +EG  +++ LL++N  ++  +A      +H  ++  H  + ++L +       +T +G+ P+H A   G L ++K L++
Sbjct:  656 GFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVE 743          

HSP 16 Score: 50.447 bits (119), Expect = 1.481e-7
Identity = 23/90 (25.56%), Postives = 49/90 (54.44%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            ++G   +H+  +E  V VA++L +N   I+ +    + P+H A   G   ++K L++   +   +T++ + P+H A+  GH   ++ LL+
Sbjct:  687 NNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLE 776          

HSP 17 Score: 49.2914 bits (116), Expect = 3.351e-7
Identity = 22/94 (23.40%), Postives = 51/94 (54.26%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            K    +G+ P+HIA ++  +++A  LL    + + +++  + P+H +++ GH EI  LL++   +   +  +G   +H  +   H+ + +IL +
Sbjct:  617 KAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYN 710          

HSP 18 Score: 48.1358 bits (113), Expect = 8.649e-7
Identity = 27/91 (29.67%), Postives = 50/91 (54.95%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +DG  P+ +A ++G   V  +LL+N    D + K +   +H A++   +    LLL    N +  +KSG+ P+H A+  GH  + ++LL++
Sbjct:  163 EDGFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEK 249          
BLAST of SMED30027856 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 72.0182 bits (175), Expect = 4.877e-15
Identity = 33/86 (38.37%), Postives = 52/86 (60.47%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+HIA R G  D+  LLL    N +   +  + P+H A++ G  E+  +LLD N +    TK G+ P+H AS  G+LE++++LL+R
Sbjct:  494 PLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLER 579          

HSP 2 Score: 69.707 bits (169), Expect = 3.177e-14
Identity = 33/88 (37.50%), Postives = 50/88 (56.82%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A R    DV R+L+ N   +D +A+    P+H ASR G+++I+ LLL    N    T+  + P+H A+  G  E+  ILLD
Sbjct:  458 GETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLD 545          

HSP 3 Score: 63.1586 bits (152), Expect = 5.650e-12
Identity = 29/84 (34.52%), Postives = 48/84 (57.14%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            P+H+A + G  ++A LLL     ID   K    P+H A+R GH +++ LL+ +      +TK+G  P+H A+ G H++  + LL
Sbjct:  263 PLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLL 346          

HSP 4 Score: 63.1586 bits (152), Expect = 6.218e-12
Identity = 31/100 (31.00%), Postives = 53/100 (53.00%), Query Frame = 1
Query:  196 LKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            L+ E    V    G  P+HIA   G  +V +LLL+   N++ +A+    P+H A++ G + +  LLL R    +  TK    P+H A+  GH +++ +L+
Sbjct:  214 LQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLV 313          

HSP 5 Score: 62.003 bits (149), Expect = 1.313e-11
Identity = 31/89 (34.83%), Postives = 56/89 (62.92%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            D + P+HIA +EG  +VA +LLD+N +     K+ + P+H AS+ G+ E+++LLL+R    + E K+   P+H A+   + ++  +LL+
Sbjct:  523 DNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLE 611          

HSP 6 Score: 59.6918 bits (143), Expect = 8.486e-11
Identity = 28/87 (32.18%), Postives = 49/87 (56.32%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G  P+H+A + G ++V RLLL+    +D E K +  P+H A+   + ++  LLL+   + +   K+G+ P+H A+    +EI   LL
Sbjct:  557 GFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLL 643          

HSP 7 Score: 55.8398 bits (133), Expect = 1.822e-9
Identity = 30/110 (27.27%), Postives = 58/110 (52.73%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G++E VRE +K +         G+  +HIA   G   +  +L++N  N++ ++   + P++ A++  H E++K LL    N    T+ G+ P+  A   GH  ++ +LL+
Sbjct:   77 GHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLE 186          

HSP 8 Score: 55.0694 bits (131), Expect = 3.553e-9
Identity = 29/86 (33.72%), Postives = 48/86 (55.81%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H+A   G V VA+LLLD + + +  A   + P+H A +    ++++LLL      E  T+SG  P+H A+  G + I+  LL +
Sbjct:  362 PLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQ 447          

HSP 9 Score: 55.0694 bits (131), Expect = 3.621e-9
Identity = 30/109 (27.52%), Postives = 56/109 (51.38%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+ E V E L+        + +G   +H+A +EG  +V R L+     +D   ++    +H AS  G S I+ +L++   N   ++ +G+ P++ A+   H E++K LL
Sbjct:   44 GDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLL 152          

HSP 10 Score: 54.6842 bits (130), Expect = 4.299e-9
Identity = 28/88 (31.82%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +   VD AR LL +   +D        P+H A+  GH  + KLLLDR+ +      +G+ P+H A     ++++++LL
Sbjct:  325 NGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLL 412          

HSP 11 Score: 54.299 bits (129), Expect = 6.295e-9
Identity = 27/85 (31.76%), Postives = 44/85 (51.76%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA ++     A LLL N  N D  +K  + P+H A+  GH  + +LLL++  N   + +    P+H A+  G   +  +LL R
Sbjct:  198 LHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSR 282          

HSP 12 Score: 53.9138 bits (128), Expect = 8.220e-9
Identity = 35/111 (31.53%), Postives = 58/111 (52.25%), Query Frame = 1
Query:  169 GNAEGVRENL-KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            GN E VR  L +G  VD +   +   P+H+A       VA LLL+N  +    AK  + P+H A++    EI   LL    +   ++++G+ P+H ++  GH EI  +L++
Sbjct:  568 GNLEVVRLLLERGTPVD-IEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIE 677          

HSP 13 Score: 53.1434 bits (126), Expect = 1.484e-8
Identity = 21/86 (24.42%), Postives = 51/86 (59.30%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+H+AC  G +++ + L++N  ++  + +  + P+H A++ GH+  ++ LL+   +   +T +G  P+  A   G++ +++ L
Sbjct:  722 GYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807          

HSP 14 Score: 53.1434 bits (126), Expect = 1.744e-8
Identity = 26/88 (29.55%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LLL     I+   +    P+H A+  G   I+  LL +  N + ET  G  P+H A+     +++++L+
Sbjct:  391 NGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI 478          

HSP 15 Score: 50.8322 bits (120), Expect = 9.666e-8
Identity = 21/88 (23.86%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H++ +EG  +++ LL++N  ++  +A      +H  ++  H  + ++L +       +T +G+ P+H A   G L ++K L++
Sbjct:  656 GFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVE 743          

HSP 16 Score: 50.447 bits (119), Expect = 1.482e-7
Identity = 23/90 (25.56%), Postives = 49/90 (54.44%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            ++G   +H+  +E  V VA++L +N   I+ +    + P+H A   G   ++K L++   +   +T++ + P+H A+  GH   ++ LL+
Sbjct:  687 NNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLE 776          

HSP 17 Score: 49.2914 bits (116), Expect = 3.353e-7
Identity = 22/94 (23.40%), Postives = 51/94 (54.26%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            K    +G+ P+HIA ++  +++A  LL    + + +++  + P+H +++ GH EI  LL++   +   +  +G   +H  +   H+ + +IL +
Sbjct:  617 KAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYN 710          

HSP 18 Score: 48.1358 bits (113), Expect = 8.656e-7
Identity = 27/91 (29.67%), Postives = 50/91 (54.95%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +DG  P+ +A ++G   V  +LL+N    D + K +   +H A++   +    LLL    N +  +KSG+ P+H A+  GH  + ++LL++
Sbjct:  163 EDGFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEK 249          
BLAST of SMED30027856 vs. Ensembl Fly
Match: Ank (gene:FBgn0011747 transcript:FBtr0089172)

HSP 1 Score: 72.4034 bits (176), Expect = 4.163e-15
Identity = 35/94 (37.23%), Postives = 53/94 (56.38%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            ++   G  P+HIA   G VD+A LLL+N  +++  AK    P+H A + G   +  LLL R    +  T+ G  P+H AS  GH+E++K LL +
Sbjct:  229 IVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQ 322          

HSP 2 Score: 68.5514 bits (166), Expect = 1.011e-13
Identity = 28/88 (31.82%), Postives = 55/88 (62.50%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +EG V V+++LL++  NI    +  + P+H A+  GH +++K  ++ + + E  +  G+ P+H A+  GH+ I+ +LL
Sbjct:  693 NGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLL 780          

HSP 3 Score: 67.3958 bits (163), Expect = 2.423e-13
Identity = 33/85 (38.82%), Postives = 48/85 (56.47%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            P+H+AC+ G + +  LLL     ID   +    P+H ASR GH E++K LL +N     +TK+G   +H A+ G H E   +LLD
Sbjct:  270 PLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLD 354          

HSP 4 Score: 65.0846 bits (157), Expect = 1.500e-12
Identity = 31/93 (33.33%), Postives = 55/93 (59.14%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +I   G  P+H+A + G VD+ +LLL+    I   AK    P+H A++ GH  + ++LL+   N    T++G+ P+H A+  GHL+++K  ++
Sbjct:  657 IISKSGFSPLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIE 748          

HSP 5 Score: 62.003 bits (149), Expect = 1.711e-11
Identity = 28/89 (31.46%), Postives = 57/89 (64.04%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +G   IHIAC++ ++++A  LL +  +++  +K  + P+H A++GG+ ++++LLL+         K+G  P+H A+  GH+ + +ILL+
Sbjct:  628 NGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLE 715          

HSP 6 Score: 61.6178 bits (148), Expect = 2.533e-11
Identity = 33/107 (30.84%), Postives = 59/107 (55.14%), Query Frame = 1
Query:  175 AEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            A+ +R  L+   VD  I  +G  P+H+A R G +++  LLL +   I+ ++  K+  +H A++ G   I+++LL+        TK G+ P+H A   G   +++ILL
Sbjct:  478 ADIIRILLRSAKVD-AIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILL 583          

HSP 7 Score: 57.3806 bits (137), Expect = 6.420e-10
Identity = 26/87 (29.89%), Postives = 51/87 (58.62%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            +G+ P+H+A   G +D+ +  ++N+ +I+  +   + P+H A++ GH  I+ LLL    N    TK G   +H AS+ G++ +++ L
Sbjct:  726 NGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDGNTALHIASNLGYVTVMESL 812          

HSP 8 Score: 54.299 bits (129), Expect = 9.024e-9
Identity = 32/128 (25.00%), Postives = 60/128 (46.88%), Query Frame = 1
Query:  214 DKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNC-------NFECE--------------------------TKSGWRPVHWASSGGHLEILKILLD 498
            +  +   G  P+H+AC+ G  +V ++LL N  +ID + K    P+H A+   +  I++LLL           N +C                           +KSG+ P+H A+ GG+++++++LL+
Sbjct:  556 NNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLE 683          

HSP 9 Score: 52.7582 bits (125), Expect = 2.724e-8
Identity = 29/83 (34.94%), Postives = 45/83 (54.22%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A   G V VA+LLLD   N +  A   + P+H A +    ++++LL+    N    T+SG  P+H AS  G + I+  LL
Sbjct:  370 LHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLL 452          

HSP 10 Score: 52.7582 bits (125), Expect = 2.830e-8
Identity = 27/88 (30.68%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A R    D+ R+LL  +  +D  A+    P+H ASR G+  I+ LLL        ++   +  +H A+  G   I+++LL+
Sbjct:  465 GETPLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLE 551          

HSP 11 Score: 51.9878 bits (123), Expect = 5.723e-8
Identity = 27/88 (30.68%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + +  LL+ +  NI    +    P+H AS  G   I+  LL    + +  T  G  P+H A+     +I++ILL
Sbjct:  398 NGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILL 485          

HSP 12 Score: 50.8322 bits (120), Expect = 1.236e-7
Identity = 26/89 (29.21%), Postives = 49/89 (55.06%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            + G  P+H+A   G +++   LL +  + D    R   P+H A+R   ++I+++LL R+   +   + G  P+H AS  G++ I+ +LL
Sbjct:  430 ESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLL 517          

HSP 13 Score: 50.8322 bits (120), Expect = 1.321e-7
Identity = 28/85 (32.94%), Postives = 46/85 (54.12%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA ++  V+ A+LLL ++ N D  +K  + P+H A+  G+ +I  LLL+   +     K    P+H A   G L +  +LL R
Sbjct:  205 LHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCR 289          

HSP 14 Score: 49.2914 bits (116), Expect = 3.791e-7
Identity = 23/89 (25.84%), Postives = 51/89 (57.30%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +D +  +HIA +EG  ++ ++LL+N    +   K+ + P+H A + G   ++++LL    + + + K+   P+H A+   +  I+++LL
Sbjct:  528 NDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLL 616          

HSP 15 Score: 48.9062 bits (115), Expect = 6.762e-7
Identity = 27/90 (30.00%), Postives = 50/90 (55.56%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +DG  P+ +A ++G   +  +LL+N    D   K +   +H A++       KLLL  + N +  +KSG+ P+H A+  G+++I  +LL+
Sbjct:  170 EDGFTPLAVAMQQGHDKIVAVLLEN----DVRGKVRLPALHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLN 255          

HSP 16 Score: 46.595 bits (109), Expect = 3.290e-6
Identity = 24/88 (27.27%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G   +H+A ++G+VD+   LL     ID   K+    +H AS  G  +++  L+  N N   ++ +G+ P++ A+   H    + LL
Sbjct:   72 NGLNALHLAAKDGYVDICCELLRRGIKIDNATKKGNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLL 159          

HSP 17 Score: 45.4394 bits (106), Expect = 8.154e-6
Identity = 25/88 (28.41%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G+  +HIA   G  DV   L+  N N++ ++   + P++ A++  H    + LL    N    T+ G+ P+  A   GH +I+ +LL+
Sbjct:  106 GNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTPLAVAMQQGHDKIVAVLLE 193          
BLAST of SMED30027856 vs. Ensembl Fly
Match: Ank (gene:FBgn0011747 transcript:FBtr0300497)

HSP 1 Score: 72.4034 bits (176), Expect = 4.163e-15
Identity = 35/94 (37.23%), Postives = 53/94 (56.38%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            ++   G  P+HIA   G VD+A LLL+N  +++  AK    P+H A + G   +  LLL R    +  T+ G  P+H AS  GH+E++K LL +
Sbjct:  229 IVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQ 322          

HSP 2 Score: 68.5514 bits (166), Expect = 1.011e-13
Identity = 28/88 (31.82%), Postives = 55/88 (62.50%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +EG V V+++LL++  NI    +  + P+H A+  GH +++K  ++ + + E  +  G+ P+H A+  GH+ I+ +LL
Sbjct:  693 NGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLL 780          

HSP 3 Score: 67.3958 bits (163), Expect = 2.423e-13
Identity = 33/85 (38.82%), Postives = 48/85 (56.47%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            P+H+AC+ G + +  LLL     ID   +    P+H ASR GH E++K LL +N     +TK+G   +H A+ G H E   +LLD
Sbjct:  270 PLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLD 354          

HSP 4 Score: 65.0846 bits (157), Expect = 1.500e-12
Identity = 31/93 (33.33%), Postives = 55/93 (59.14%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +I   G  P+H+A + G VD+ +LLL+    I   AK    P+H A++ GH  + ++LL+   N    T++G+ P+H A+  GHL+++K  ++
Sbjct:  657 IISKSGFSPLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIE 748          

HSP 5 Score: 62.003 bits (149), Expect = 1.711e-11
Identity = 28/89 (31.46%), Postives = 57/89 (64.04%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +G   IHIAC++ ++++A  LL +  +++  +K  + P+H A++GG+ ++++LLL+         K+G  P+H A+  GH+ + +ILL+
Sbjct:  628 NGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLE 715          

HSP 6 Score: 61.6178 bits (148), Expect = 2.533e-11
Identity = 33/107 (30.84%), Postives = 59/107 (55.14%), Query Frame = 1
Query:  175 AEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            A+ +R  L+   VD  I  +G  P+H+A R G +++  LLL +   I+ ++  K+  +H A++ G   I+++LL+        TK G+ P+H A   G   +++ILL
Sbjct:  478 ADIIRILLRSAKVD-AIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILL 583          

HSP 7 Score: 57.3806 bits (137), Expect = 6.420e-10
Identity = 26/87 (29.89%), Postives = 51/87 (58.62%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            +G+ P+H+A   G +D+ +  ++N+ +I+  +   + P+H A++ GH  I+ LLL    N    TK G   +H AS+ G++ +++ L
Sbjct:  726 NGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDGNTALHIASNLGYVTVMESL 812          

HSP 8 Score: 54.299 bits (129), Expect = 9.024e-9
Identity = 32/128 (25.00%), Postives = 60/128 (46.88%), Query Frame = 1
Query:  214 DKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNC-------NFECE--------------------------TKSGWRPVHWASSGGHLEILKILLD 498
            +  +   G  P+H+AC+ G  +V ++LL N  +ID + K    P+H A+   +  I++LLL           N +C                           +KSG+ P+H A+ GG+++++++LL+
Sbjct:  556 NNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLE 683          

HSP 9 Score: 52.7582 bits (125), Expect = 2.724e-8
Identity = 29/83 (34.94%), Postives = 45/83 (54.22%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A   G V VA+LLLD   N +  A   + P+H A +    ++++LL+    N    T+SG  P+H AS  G + I+  LL
Sbjct:  370 LHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLL 452          

HSP 10 Score: 52.7582 bits (125), Expect = 2.830e-8
Identity = 27/88 (30.68%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A R    D+ R+LL  +  +D  A+    P+H ASR G+  I+ LLL        ++   +  +H A+  G   I+++LL+
Sbjct:  465 GETPLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLE 551          

HSP 11 Score: 51.9878 bits (123), Expect = 5.723e-8
Identity = 27/88 (30.68%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + +  LL+ +  NI    +    P+H AS  G   I+  LL    + +  T  G  P+H A+     +I++ILL
Sbjct:  398 NGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILL 485          

HSP 12 Score: 50.8322 bits (120), Expect = 1.236e-7
Identity = 26/89 (29.21%), Postives = 49/89 (55.06%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            + G  P+H+A   G +++   LL +  + D    R   P+H A+R   ++I+++LL R+   +   + G  P+H AS  G++ I+ +LL
Sbjct:  430 ESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLL 517          

HSP 13 Score: 50.8322 bits (120), Expect = 1.321e-7
Identity = 28/85 (32.94%), Postives = 46/85 (54.12%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA ++  V+ A+LLL ++ N D  +K  + P+H A+  G+ +I  LLL+   +     K    P+H A   G L +  +LL R
Sbjct:  205 LHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCR 289          

HSP 14 Score: 49.2914 bits (116), Expect = 3.791e-7
Identity = 23/89 (25.84%), Postives = 51/89 (57.30%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +D +  +HIA +EG  ++ ++LL+N    +   K+ + P+H A + G   ++++LL    + + + K+   P+H A+   +  I+++LL
Sbjct:  528 NDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLL 616          

HSP 15 Score: 48.9062 bits (115), Expect = 6.762e-7
Identity = 27/90 (30.00%), Postives = 50/90 (55.56%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +DG  P+ +A ++G   +  +LL+N    D   K +   +H A++       KLLL  + N +  +KSG+ P+H A+  G+++I  +LL+
Sbjct:  170 EDGFTPLAVAMQQGHDKIVAVLLEN----DVRGKVRLPALHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLN 255          

HSP 16 Score: 46.595 bits (109), Expect = 3.290e-6
Identity = 24/88 (27.27%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G   +H+A ++G+VD+   LL     ID   K+    +H AS  G  +++  L+  N N   ++ +G+ P++ A+   H    + LL
Sbjct:   72 NGLNALHLAAKDGYVDICCELLRRGIKIDNATKKGNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLL 159          

HSP 17 Score: 45.4394 bits (106), Expect = 8.154e-6
Identity = 25/88 (28.41%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G+  +HIA   G  DV   L+  N N++ ++   + P++ A++  H    + LL    N    T+ G+ P+  A   GH +I+ +LL+
Sbjct:  106 GNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTPLAVAMQQGHDKIVAVLLE 193          
BLAST of SMED30027856 vs. Ensembl Fly
Match: Ank (gene:FBgn0011747 transcript:FBtr0089171)

HSP 1 Score: 72.4034 bits (176), Expect = 4.163e-15
Identity = 35/94 (37.23%), Postives = 53/94 (56.38%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            ++   G  P+HIA   G VD+A LLL+N  +++  AK    P+H A + G   +  LLL R    +  T+ G  P+H AS  GH+E++K LL +
Sbjct:  229 IVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQ 322          

HSP 2 Score: 68.5514 bits (166), Expect = 1.011e-13
Identity = 28/88 (31.82%), Postives = 55/88 (62.50%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +EG V V+++LL++  NI    +  + P+H A+  GH +++K  ++ + + E  +  G+ P+H A+  GH+ I+ +LL
Sbjct:  693 NGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLL 780          

HSP 3 Score: 67.3958 bits (163), Expect = 2.423e-13
Identity = 33/85 (38.82%), Postives = 48/85 (56.47%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            P+H+AC+ G + +  LLL     ID   +    P+H ASR GH E++K LL +N     +TK+G   +H A+ G H E   +LLD
Sbjct:  270 PLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLD 354          

HSP 4 Score: 65.0846 bits (157), Expect = 1.500e-12
Identity = 31/93 (33.33%), Postives = 55/93 (59.14%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +I   G  P+H+A + G VD+ +LLL+    I   AK    P+H A++ GH  + ++LL+   N    T++G+ P+H A+  GHL+++K  ++
Sbjct:  657 IISKSGFSPLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIE 748          

HSP 5 Score: 62.003 bits (149), Expect = 1.711e-11
Identity = 28/89 (31.46%), Postives = 57/89 (64.04%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +G   IHIAC++ ++++A  LL +  +++  +K  + P+H A++GG+ ++++LLL+         K+G  P+H A+  GH+ + +ILL+
Sbjct:  628 NGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLE 715          

HSP 6 Score: 61.6178 bits (148), Expect = 2.533e-11
Identity = 33/107 (30.84%), Postives = 59/107 (55.14%), Query Frame = 1
Query:  175 AEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            A+ +R  L+   VD  I  +G  P+H+A R G +++  LLL +   I+ ++  K+  +H A++ G   I+++LL+        TK G+ P+H A   G   +++ILL
Sbjct:  478 ADIIRILLRSAKVD-AIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILL 583          

HSP 7 Score: 57.3806 bits (137), Expect = 6.420e-10
Identity = 26/87 (29.89%), Postives = 51/87 (58.62%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            +G+ P+H+A   G +D+ +  ++N+ +I+  +   + P+H A++ GH  I+ LLL    N    TK G   +H AS+ G++ +++ L
Sbjct:  726 NGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDGNTALHIASNLGYVTVMESL 812          

HSP 8 Score: 54.299 bits (129), Expect = 9.024e-9
Identity = 32/128 (25.00%), Postives = 60/128 (46.88%), Query Frame = 1
Query:  214 DKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNC-------NFECE--------------------------TKSGWRPVHWASSGGHLEILKILLD 498
            +  +   G  P+H+AC+ G  +V ++LL N  +ID + K    P+H A+   +  I++LLL           N +C                           +KSG+ P+H A+ GG+++++++LL+
Sbjct:  556 NNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLE 683          

HSP 9 Score: 52.7582 bits (125), Expect = 2.724e-8
Identity = 29/83 (34.94%), Postives = 45/83 (54.22%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A   G V VA+LLLD   N +  A   + P+H A +    ++++LL+    N    T+SG  P+H AS  G + I+  LL
Sbjct:  370 LHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLL 452          

HSP 10 Score: 52.7582 bits (125), Expect = 2.830e-8
Identity = 27/88 (30.68%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A R    D+ R+LL  +  +D  A+    P+H ASR G+  I+ LLL        ++   +  +H A+  G   I+++LL+
Sbjct:  465 GETPLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLE 551          

HSP 11 Score: 51.9878 bits (123), Expect = 5.723e-8
Identity = 27/88 (30.68%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + +  LL+ +  NI    +    P+H AS  G   I+  LL    + +  T  G  P+H A+     +I++ILL
Sbjct:  398 NGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILL 485          

HSP 12 Score: 50.8322 bits (120), Expect = 1.236e-7
Identity = 26/89 (29.21%), Postives = 49/89 (55.06%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            + G  P+H+A   G +++   LL +  + D    R   P+H A+R   ++I+++LL R+   +   + G  P+H AS  G++ I+ +LL
Sbjct:  430 ESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLL 517          

HSP 13 Score: 50.8322 bits (120), Expect = 1.321e-7
Identity = 28/85 (32.94%), Postives = 46/85 (54.12%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA ++  V+ A+LLL ++ N D  +K  + P+H A+  G+ +I  LLL+   +     K    P+H A   G L +  +LL R
Sbjct:  205 LHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCR 289          

HSP 14 Score: 49.2914 bits (116), Expect = 3.791e-7
Identity = 23/89 (25.84%), Postives = 51/89 (57.30%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +D +  +HIA +EG  ++ ++LL+N    +   K+ + P+H A + G   ++++LL    + + + K+   P+H A+   +  I+++LL
Sbjct:  528 NDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLL 616          

HSP 15 Score: 48.9062 bits (115), Expect = 6.762e-7
Identity = 27/90 (30.00%), Postives = 50/90 (55.56%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +DG  P+ +A ++G   +  +LL+N    D   K +   +H A++       KLLL  + N +  +KSG+ P+H A+  G+++I  +LL+
Sbjct:  170 EDGFTPLAVAMQQGHDKIVAVLLEN----DVRGKVRLPALHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLN 255          

HSP 16 Score: 46.595 bits (109), Expect = 3.290e-6
Identity = 24/88 (27.27%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G   +H+A ++G+VD+   LL     ID   K+    +H AS  G  +++  L+  N N   ++ +G+ P++ A+   H    + LL
Sbjct:   72 NGLNALHLAAKDGYVDICCELLRRGIKIDNATKKGNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLL 159          

HSP 17 Score: 45.4394 bits (106), Expect = 8.154e-6
Identity = 25/88 (28.41%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G+  +HIA   G  DV   L+  N N++ ++   + P++ A++  H    + LL    N    T+ G+ P+  A   GH +I+ +LL+
Sbjct:  106 GNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTPLAVAMQQGHDKIVAVLLE 193          
BLAST of SMED30027856 vs. Ensembl Fly
Match: Ank (gene:FBgn0011747 transcript:FBtr0089173)

HSP 1 Score: 72.4034 bits (176), Expect = 4.163e-15
Identity = 35/94 (37.23%), Postives = 53/94 (56.38%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            ++   G  P+HIA   G VD+A LLL+N  +++  AK    P+H A + G   +  LLL R    +  T+ G  P+H AS  GH+E++K LL +
Sbjct:  229 IVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQ 322          

HSP 2 Score: 68.5514 bits (166), Expect = 1.011e-13
Identity = 28/88 (31.82%), Postives = 55/88 (62.50%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +EG V V+++LL++  NI    +  + P+H A+  GH +++K  ++ + + E  +  G+ P+H A+  GH+ I+ +LL
Sbjct:  693 NGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLL 780          

HSP 3 Score: 67.3958 bits (163), Expect = 2.423e-13
Identity = 33/85 (38.82%), Postives = 48/85 (56.47%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            P+H+AC+ G + +  LLL     ID   +    P+H ASR GH E++K LL +N     +TK+G   +H A+ G H E   +LLD
Sbjct:  270 PLHVACKWGKLSLCTLLLCRGAKIDAATRDGLTPLHCASRSGHVEVIKHLLQQNAPILTKTKNGLSALHMAAQGEHDEAAHLLLD 354          

HSP 4 Score: 65.0846 bits (157), Expect = 1.500e-12
Identity = 31/93 (33.33%), Postives = 55/93 (59.14%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +I   G  P+H+A + G VD+ +LLL+    I   AK    P+H A++ GH  + ++LL+   N    T++G+ P+H A+  GHL+++K  ++
Sbjct:  657 IISKSGFSPLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLEHGANISERTRNGYTPLHMAAHYGHLDLVKFFIE 748          

HSP 5 Score: 62.003 bits (149), Expect = 1.711e-11
Identity = 28/89 (31.46%), Postives = 57/89 (64.04%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +G   IHIAC++ ++++A  LL +  +++  +K  + P+H A++GG+ ++++LLL+         K+G  P+H A+  GH+ + +ILL+
Sbjct:  628 NGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLEYGV-ISAAAKNGLTPLHVAAQEGHVLVSQILLE 715          

HSP 6 Score: 61.6178 bits (148), Expect = 2.533e-11
Identity = 33/107 (30.84%), Postives = 59/107 (55.14%), Query Frame = 1
Query:  175 AEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            A+ +R  L+   VD  I  +G  P+H+A R G +++  LLL +   I+ ++  K+  +H A++ G   I+++LL+        TK G+ P+H A   G   +++ILL
Sbjct:  478 ADIIRILLRSAKVD-AIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILL 583          

HSP 7 Score: 57.3806 bits (137), Expect = 6.420e-10
Identity = 26/87 (29.89%), Postives = 51/87 (58.62%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            +G+ P+H+A   G +D+ +  ++N+ +I+  +   + P+H A++ GH  I+ LLL    N    TK G   +H AS+ G++ +++ L
Sbjct:  726 NGYTPLHMAAHYGHLDLVKFFIENDADIEMSSNIGYTPLHQAAQQGHIMIINLLLRHKANPNALTKDGNTALHIASNLGYVTVMESL 812          

HSP 8 Score: 54.299 bits (129), Expect = 9.024e-9
Identity = 32/128 (25.00%), Postives = 60/128 (46.88%), Query Frame = 1
Query:  214 DKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNC-------NFECE--------------------------TKSGWRPVHWASSGGHLEILKILLD 498
            +  +   G  P+H+AC+ G  +V ++LL N  +ID + K    P+H A+   +  I++LLL           N +C                           +KSG+ P+H A+ GG+++++++LL+
Sbjct:  556 NNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLLKNGSSPNLCARNGQCAIHIACKKNYLEIAMQLLQHGADVNIISKSGFSPLHLAAQGGNVDMVQLLLE 683          

HSP 9 Score: 52.7582 bits (125), Expect = 2.724e-8
Identity = 29/83 (34.94%), Postives = 45/83 (54.22%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A   G V VA+LLLD   N +  A   + P+H A +    ++++LL+    N    T+SG  P+H AS  G + I+  LL
Sbjct:  370 LHVAAHCGHVKVAKLLLDYKANPNARALNGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLL 452          

HSP 10 Score: 52.7582 bits (125), Expect = 2.830e-8
Identity = 27/88 (30.68%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A R    D+ R+LL  +  +D  A+    P+H ASR G+  I+ LLL        ++   +  +H A+  G   I+++LL+
Sbjct:  465 GETPLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLLQHGAEINAQSNDKYSALHIAAKEGQENIVQVLLE 551          

HSP 11 Score: 51.9878 bits (123), Expect = 5.723e-8
Identity = 27/88 (30.68%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + +  LL+ +  NI    +    P+H AS  G   I+  LL    + +  T  G  P+H A+     +I++ILL
Sbjct:  398 NGFTPLHIACKKNRIKMVELLIKHGANIGATTESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILL 485          

HSP 12 Score: 50.8322 bits (120), Expect = 1.236e-7
Identity = 26/89 (29.21%), Postives = 49/89 (55.06%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            + G  P+H+A   G +++   LL +  + D    R   P+H A+R   ++I+++LL R+   +   + G  P+H AS  G++ I+ +LL
Sbjct:  430 ESGLTPLHVASFMGCINIVIYLLQHEASADLPTIRGETPLHLAARANQADIIRILL-RSAKVDAIAREGQTPLHVASRLGNINIIMLLL 517          

HSP 13 Score: 50.8322 bits (120), Expect = 1.321e-7
Identity = 28/85 (32.94%), Postives = 46/85 (54.12%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA ++  V+ A+LLL ++ N D  +K  + P+H A+  G+ +I  LLL+   +     K    P+H A   G L +  +LL R
Sbjct:  205 LHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLNNKADVNYVAKHNITPLHVACKWGKLSLCTLLLCR 289          

HSP 14 Score: 49.2914 bits (116), Expect = 3.791e-7
Identity = 23/89 (25.84%), Postives = 51/89 (57.30%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +D +  +HIA +EG  ++ ++LL+N    +   K+ + P+H A + G   ++++LL    + + + K+   P+H A+   +  I+++LL
Sbjct:  528 NDKYSALHIAAKEGQENIVQVLLENGAENNAVTKKGFTPLHLACKYGKQNVVQILLQNGASIDFQGKNDVTPLHVATHYNNPSIVELLL 616          

HSP 15 Score: 48.9062 bits (115), Expect = 6.762e-7
Identity = 27/90 (30.00%), Postives = 50/90 (55.56%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +DG  P+ +A ++G   +  +LL+N    D   K +   +H A++       KLLL  + N +  +KSG+ P+H A+  G+++I  +LL+
Sbjct:  170 EDGFTPLAVAMQQGHDKIVAVLLEN----DVRGKVRLPALHIAAKKNDVNAAKLLLQHDPNADIVSKSGFTPLHIAAHYGNVDIATLLLN 255          

HSP 16 Score: 46.595 bits (109), Expect = 3.290e-6
Identity = 24/88 (27.27%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G   +H+A ++G+VD+   LL     ID   K+    +H AS  G  +++  L+  N N   ++ +G+ P++ A+   H    + LL
Sbjct:   72 NGLNALHLAAKDGYVDICCELLRRGIKIDNATKKGNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLL 159          

HSP 17 Score: 45.4394 bits (106), Expect = 8.154e-6
Identity = 25/88 (28.41%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G+  +HIA   G  DV   L+  N N++ ++   + P++ A++  H    + LL    N    T+ G+ P+  A   GH +I+ +LL+
Sbjct:  106 GNTALHIASLAGQHDVINQLILYNANVNVQSLNGFTPLYMAAQENHDNCCRTLLANGANPSLSTEDGFTPLAVAMQQGHDKIVAVLLE 193          
BLAST of SMED30027856 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0334062)

HSP 1 Score: 70.0922 bits (170), Expect = 2.859e-14
Identity = 31/94 (32.98%), Postives = 55/94 (58.51%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            V    G  P+HIA   G  ++A LL+    +++  AK    P+H A++ G + ++ LLL++  N E +T+ G  P+H A+  GH +++ +LL+R
Sbjct:  200 VTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLER 293          

HSP 2 Score: 69.3218 bits (168), Expect = 4.758e-14
Identity = 29/84 (34.52%), Postives = 52/84 (61.90%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            P+H+A + G  ++  LLL+   NI+ + +    P+H A+R GH +++ +LL+R      +TK+G  P+H A+ G H++  +ILL
Sbjct:  241 PLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILL 324          

HSP 3 Score: 59.6918 bits (143), Expect = 1.053e-10
Identity = 28/82 (34.15%), Postives = 44/82 (53.66%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEI 480
            G  P+H+A R    D+ R+LL N   +D  A+ +  P+H ASR G+ +I+ LLL      +  TK  +  +H A+  G  E+
Sbjct:  436 GETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEV 517          

HSP 4 Score: 57.3806 bits (137), Expect = 7.197e-10
Identity = 32/112 (28.57%), Postives = 63/112 (56.25%), Query Frame = 1
Query:  166 NGNAEGVRENLK-GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +G+   V E L+ G  VD      G+  +HIA   G  +V +LLL++N +++ +++  + P++ A++  H  +++LLL    N    T+ G+ P+  A   GH +++ +LL+
Sbjct:   54 DGHIHVVSELLRRGAIVDSATKK-GNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLE 164          

HSP 5 Score: 53.1434 bits (126), Expect = 1.679e-8
Identity = 28/83 (33.73%), Postives = 46/83 (55.42%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A   G V VA+LLLD N + +  A   + P+H A +    ++++LLL    +    T+SG  P+H A+  G + I+  LL
Sbjct:  341 LHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLL 423          

HSP 6 Score: 51.2174 bits (121), Expect = 9.324e-8
Identity = 27/88 (30.68%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LLL +  +I    +    P+H A+  G   I+  LL  + + +  T  G  P+H A+     +I++ILL
Sbjct:  369 NGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILL 456          

HSP 7 Score: 50.447 bits (119), Expect = 1.588e-7
Identity = 24/89 (26.97%), Postives = 48/89 (53.93%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            + G  P+H+A   G +++   LL ++ + D    R   P+H A+R   ++I+++LL      +   +    P+H AS  G+++I+ +LL
Sbjct:  401 ESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLL 489          

HSP 8 Score: 46.2098 bits (108), Expect = 4.002e-6
Identity = 27/91 (29.67%), Postives = 49/91 (53.85%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +DG  P+ +A ++G   V  +LL++    D   K +   +H A++    +   LLLD + N +  +KSG+ P+H AS  G+  I  +L+ +
Sbjct:  141 EDGFTPLAVAMQQGHDKVVAVLLES----DTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQK 227          
BLAST of SMED30027856 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 80.8777 bits (198), Expect = 6.100e-18
Identity = 36/85 (42.35%), Postives = 54/85 (63.53%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            P+H+A R G  +VA+ LL NN  +D +AK    P+H A+R GH E++KLL++   N +  T +G  P+H A+  GH +  +ILLD
Sbjct:  381 PLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLD 465          

HSP 2 Score: 78.9518 bits (193), Expect = 2.767e-17
Identity = 35/88 (39.77%), Postives = 56/88 (63.64%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIA R G V + RLLLD    ID + K +  P+H A+R GH  ++++LLD+    + +TK+G  P+H A+ G H++ ++ LL
Sbjct:  179 NGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLL 266          

HSP 3 Score: 71.633 bits (174), Expect = 1.100e-14
Identity = 35/94 (37.23%), Postives = 56/94 (59.57%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            V+   G  P+HIA     + VA+LLL+   N++   K    P+H ASR G+  +++LLLDR    + +TK    P+H A+  GH+ +++ILLD+
Sbjct:  142 VLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQ 235          

HSP 4 Score: 69.707 bits (169), Expect = 5.743e-14
Identity = 32/89 (35.96%), Postives = 52/89 (58.43%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            GH P+HIA REG     R+LLD N       K+ + P+H A + G  ++++LLL+R  N     K+G  P+H A    +L+++K+L+ +
Sbjct:  444 GHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSK 532          

HSP 5 Score: 67.0106 bits (162), Expect = 4.314e-13
Identity = 39/128 (30.47%), Postives = 66/128 (51.56%), Query Frame = 1
Query:  118 GNIEMGEFECFKEQLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G+ E+ +F      L+  NA+          VD    DD   P+H A R G  ++ +LL+++  N D        P+H A+R GH++  ++LLD N      TK G+ P+H A   G ++++++LL+R
Sbjct:  389 GHCEVAQF------LLQNNAQ----------VDAKAKDD-QTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLER 499          

HSP 6 Score: 61.2326 bits (147), Expect = 4.775e-11
Identity = 30/86 (34.88%), Postives = 46/86 (53.49%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H A R G V V  +LLD    +  + K    PIH A++G H + ++ LL  N   +  T     P+H A+  GH  ++K+LLD+
Sbjct:  216 PLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDK 301          

HSP 7 Score: 60.4622 bits (145), Expect = 9.242e-11
Identity = 40/123 (32.52%), Postives = 59/123 (47.97%), Query Frame = 1
Query:  142 ECFKEQLMNGNAEGVRENLKGEFVDKVIDD---DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +C + QL+  NAE              IDD   D   P+H+A   G   + ++LLD     +  A   + P+H A +  H   + LLL  + + E  T+SG  P+H A+  GHL I+K LL R
Sbjct:  260 DCVR-QLLQYNAE--------------IDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQR 367          

HSP 8 Score: 60.077 bits (144), Expect = 1.108e-10
Identity = 26/94 (27.66%), Postives = 51/94 (54.26%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            + + + G  P+H+A   G +++ + LL    + +    +   P+H A+R GH E+ + LL  N   + + K    P+H A+  GH E++K+L++
Sbjct:  339 EAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLME 432          

HSP 9 Score: 59.3066 bits (142), Expect = 1.754e-10
Identity = 25/90 (27.78%), Postives = 53/90 (58.89%), Query Frame = 1
Query:  226 DDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            + +G  P+H+  +EG V +A +L+    ++   ++  + P+H A   G+ +++K LL +  +   +T+ G+ P+H A+  GH +I+ +LL
Sbjct:  606 NKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLL 695          

HSP 10 Score: 56.6102 bits (135), Expect = 1.859e-9
Identity = 30/85 (35.29%), Postives = 49/85 (57.65%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R      A +LL N+ N D  +K  + P+H A+   +  + +LLL+R  N     K+G  P+H AS  G++ ++++LLDR
Sbjct:  118 LHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDR 202          

HSP 11 Score: 55.8398 bits (133), Expect = 3.296e-9
Identity = 31/109 (28.44%), Postives = 53/109 (48.62%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+A+  R  L        +   G  P+H+AC+ G VDV  LLL+   N +   K    P+H A    + +++KLL+ +  +     ++G+  +H A+    LE+   LL
Sbjct:  455 GHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLL 563          

HSP 12 Score: 55.0694 bits (131), Expect = 6.676e-9
Identity = 26/90 (28.89%), Postives = 50/90 (55.56%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+  +HIA ++  ++VA  LL    N + E+ +   P+H AS+ G  +++ LL+ +  N     K+G  P+H  +  GH+ I  +L+ +
Sbjct:  542 NGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQ 631          

HSP 13 Score: 54.6842 bits (130), Expect = 7.275e-9
Identity = 28/88 (31.82%), Postives = 44/88 (50.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  PIH+A +   +D  R LL  N  ID        P+H A+  GH  ++K+LLD+         +G+ P+H A    H+  + +LL
Sbjct:  245 NGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLL 332          

HSP 14 Score: 53.9138 bits (128), Expect = 1.667e-8
Identity = 25/89 (28.09%), Postives = 49/89 (55.06%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  P+H+A +EG  D+  LL+    N++   K    P+H  ++ GH  I  +L+ +  +    ++ G+ P+H A   G+++++K LL +
Sbjct:  576 GITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQ 664          

HSP 15 Score: 53.5286 bits (127), Expect = 2.017e-8
Identity = 27/88 (30.68%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  +    LLL ++ +++   +    P+H A+  GH  I+K LL R  +          P+H A+  GH E+ + LL
Sbjct:  311 NGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLL 398          

HSP 16 Score: 53.1434 bits (126), Expect = 2.815e-8
Identity = 23/86 (26.74%), Postives = 48/86 (55.81%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+H+AC  G + + + LL    +++ + +  + P+H A++ GH++I+ LLL         T +G  P+  A   G++ ++ +L
Sbjct:  642 GYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 727          
BLAST of SMED30027856 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 80.8777 bits (198), Expect = 6.158e-18
Identity = 36/85 (42.35%), Postives = 54/85 (63.53%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            P+H+A R G  +VA+ LL NN  +D +AK    P+H A+R GH E++KLL++   N +  T +G  P+H A+  GH +  +ILLD
Sbjct:  473 PLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLD 557          

HSP 2 Score: 78.9518 bits (193), Expect = 2.794e-17
Identity = 35/88 (39.77%), Postives = 56/88 (63.64%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIA R G V + RLLLD    ID + K +  P+H A+R GH  ++++LLD+    + +TK+G  P+H A+ G H++ ++ LL
Sbjct:  271 NGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLL 358          

HSP 3 Score: 71.633 bits (174), Expect = 1.110e-14
Identity = 35/94 (37.23%), Postives = 56/94 (59.57%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            V+   G  P+HIA     + VA+LLL+   N++   K    P+H ASR G+  +++LLLDR    + +TK    P+H A+  GH+ +++ILLD+
Sbjct:  234 VLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQ 327          

HSP 4 Score: 69.707 bits (169), Expect = 5.797e-14
Identity = 32/89 (35.96%), Postives = 52/89 (58.43%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            GH P+HIA REG     R+LLD N       K+ + P+H A + G  ++++LLL+R  N     K+G  P+H A    +L+++K+L+ +
Sbjct:  536 GHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSK 624          

HSP 5 Score: 67.0106 bits (162), Expect = 4.354e-13
Identity = 39/128 (30.47%), Postives = 66/128 (51.56%), Query Frame = 1
Query:  118 GNIEMGEFECFKEQLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G+ E+ +F      L+  NA+          VD    DD   P+H A R G  ++ +LL+++  N D        P+H A+R GH++  ++LLD N      TK G+ P+H A   G ++++++LL+R
Sbjct:  481 GHCEVAQF------LLQNNAQ----------VDAKAKDD-QTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLER 591          

HSP 6 Score: 61.2326 bits (147), Expect = 4.819e-11
Identity = 30/86 (34.88%), Postives = 46/86 (53.49%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H A R G V V  +LLD    +  + K    PIH A++G H + ++ LL  N   +  T     P+H A+  GH  ++K+LLD+
Sbjct:  308 PLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDK 393          

HSP 7 Score: 60.4622 bits (145), Expect = 9.327e-11
Identity = 40/123 (32.52%), Postives = 59/123 (47.97%), Query Frame = 1
Query:  142 ECFKEQLMNGNAEGVRENLKGEFVDKVIDD---DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +C + QL+  NAE              IDD   D   P+H+A   G   + ++LLD     +  A   + P+H A +  H   + LLL  + + E  T+SG  P+H A+  GHL I+K LL R
Sbjct:  352 DCVR-QLLQYNAE--------------IDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQR 459          

HSP 8 Score: 60.077 bits (144), Expect = 1.119e-10
Identity = 26/94 (27.66%), Postives = 51/94 (54.26%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            + + + G  P+H+A   G +++ + LL    + +    +   P+H A+R GH E+ + LL  N   + + K    P+H A+  GH E++K+L++
Sbjct:  431 EAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLME 524          

HSP 9 Score: 57.3806 bits (137), Expect = 9.431e-10
Identity = 28/111 (25.23%), Postives = 60/111 (54.05%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +GN E   +++K      + + +G   +H+A +EG V +   LL N  +++   K+    +H A+  G  +++  L++   N   +++ G+ P++ A+   HLE++K LL+
Sbjct:   55 SGNLEKALDHIKNGIDINIANQNGLNGLHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLE 165          

HSP 10 Score: 56.6102 bits (135), Expect = 1.876e-9
Identity = 30/85 (35.29%), Postives = 49/85 (57.65%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R      A +LL N+ N D  +K  + P+H A+   +  + +LLL+R  N     K+G  P+H AS  G++ ++++LLDR
Sbjct:  210 LHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDR 294          

HSP 11 Score: 55.8398 bits (133), Expect = 3.326e-9
Identity = 31/109 (28.44%), Postives = 53/109 (48.62%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+A+  R  L        +   G  P+H+AC+ G VDV  LLL+   N +   K    P+H A    + +++KLL+ +  +     ++G+  +H A+    LE+   LL
Sbjct:  547 GHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLL 655          

HSP 12 Score: 55.0694 bits (131), Expect = 6.737e-9
Identity = 26/90 (28.89%), Postives = 50/90 (55.56%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+  +HIA ++  ++VA  LL    N + E+ +   P+H AS+ G  +++ LL+ +  N     K+G  P+H  +  GH+ I  +L+ +
Sbjct:  634 NGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQ 723          

HSP 13 Score: 54.6842 bits (130), Expect = 7.340e-9
Identity = 28/88 (31.82%), Postives = 44/88 (50.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  PIH+A +   +D  R LL  N  ID        P+H A+  GH  ++K+LLD+         +G+ P+H A    H+  + +LL
Sbjct:  337 NGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLL 424          

HSP 14 Score: 53.9138 bits (128), Expect = 1.682e-8
Identity = 25/89 (28.09%), Postives = 49/89 (55.06%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  P+H+A +EG  D+  LL+    N++   K    P+H  ++ GH  I  +L+ +  +    ++ G+ P+H A   G+++++K LL +
Sbjct:  668 GITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQ 756          

HSP 15 Score: 53.5286 bits (127), Expect = 2.035e-8
Identity = 27/88 (30.68%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  +    LLL ++ +++   +    P+H A+  GH  I+K LL R  +          P+H A+  GH E+ + LL
Sbjct:  403 NGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLL 490          
BLAST of SMED30027856 vs. Ensembl Zebrafish
Match: BX640476.1 (ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:797662])

HSP 1 Score: 80.8777 bits (198), Expect = 6.284e-18
Identity = 36/85 (42.35%), Postives = 54/85 (63.53%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            P+H+A R G  +VA+ LL NN  +D +AK    P+H A+R GH E++KLL++   N +  T +G  P+H A+  GH +  +ILLD
Sbjct:  426 PLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLD 510          

HSP 2 Score: 68.5514 bits (166), Expect = 1.263e-13
Identity = 32/89 (35.96%), Postives = 51/89 (57.30%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            GH P+HIA REG     R+LLD N       K+ + P+H A + G  ++ +LLL+R  N     K+G  P+H A    +L+++K+L+ +
Sbjct:  489 GHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSK 577          

HSP 3 Score: 66.2402 bits (160), Expect = 9.478e-13
Identity = 39/128 (30.47%), Postives = 65/128 (50.78%), Query Frame = 1
Query:  118 GNIEMGEFECFKEQLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G+ E+ +F      L+  NA+          VD    DD   P+H A R G  ++ +LL+++  N D        P+H A+R GH++  ++LLD N      TK G+ P+H A   G +++ ++LL+R
Sbjct:  434 GHCEVAQF------LLQNNAQ----------VDAKAKDD-QTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVAELLLER 544          

HSP 4 Score: 60.4622 bits (145), Expect = 9.511e-11
Identity = 40/123 (32.52%), Postives = 59/123 (47.97%), Query Frame = 1
Query:  142 ECFKEQLMNGNAEGVRENLKGEFVDKVIDD---DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +C + QL+  NAE              IDD   D   P+H+A   G   + ++LLD     +  A   + P+H A +  H   + LLL  + + E  T+SG  P+H A+  GHL I+K LL R
Sbjct:  302 DCVR-QLLQYNAE--------------IDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQR 409          

HSP 5 Score: 59.3066 bits (142), Expect = 1.805e-10
Identity = 25/90 (27.78%), Postives = 53/90 (58.89%), Query Frame = 1
Query:  226 DDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            + +G  P+H+  +EG V +A +L+    ++   ++  + P+H A   G+ +++K LL +  +   +T+ G+ P+H A+  GH +I+ +LL
Sbjct:  651 NKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLL 740          

HSP 6 Score: 57.3806 bits (137), Expect = 8.573e-10
Identity = 32/109 (29.36%), Postives = 54/109 (49.54%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+A+  R  L        +   G  P+H+AC+ G VDVA LLL+   N +   K    P+H A    + +++KLL+ +  +     ++G+  +H A+    LE+   LL
Sbjct:  500 GHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLL 608          

HSP 7 Score: 55.0694 bits (131), Expect = 5.951e-9
Identity = 31/105 (29.52%), Postives = 49/105 (46.67%), Query Frame = 1
Query:  181 GVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G   N   +    +I  +G  PIH+A +   +D  R LL  N  ID        P+H A+  GH  ++K+LLD+         +G+ P+H A    H+  + +LL
Sbjct:  270 GANVNFTPKVSSTLILLNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLL 374          

HSP 8 Score: 55.0694 bits (131), Expect = 6.866e-9
Identity = 26/90 (28.89%), Postives = 50/90 (55.56%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+  +HIA ++  ++VA  LL    N + E+ +   P+H AS+ G  +++ LL+ +  N     K+G  P+H  +  GH+ I  +L+ +
Sbjct:  587 NGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQ 676          

HSP 9 Score: 53.9138 bits (128), Expect = 1.714e-8
Identity = 25/89 (28.09%), Postives = 49/89 (55.06%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  P+H+A +EG  D+  LL+    N++   K    P+H  ++ GH  I  +L+ +  +    ++ G+ P+H A   G+++++K LL +
Sbjct:  621 GITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQ 709          

HSP 10 Score: 53.1434 bits (126), Expect = 2.895e-8
Identity = 23/86 (26.74%), Postives = 48/86 (55.81%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+H+AC  G + + + LL    +++ + +  + P+H A++ GH++I+ LLL         T +G  P+  A   G++ ++ +L
Sbjct:  687 GYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 772          

HSP 11 Score: 52.7582 bits (125), Expect = 3.963e-8
Identity = 31/97 (31.96%), Postives = 49/97 (50.52%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAK--------RKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  P+HIA     + VA+LLL+   N++   K            PIH A++G H + ++ LL  N   +  T     P+H A+  GH  ++K+LLD+
Sbjct:  247 GFTPLHIAAHYENLSVAQLLLNRGANVNFTPKVSSTLILLNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDK 343          

HSP 12 Score: 52.7582 bits (125), Expect = 4.236e-8
Identity = 23/84 (27.38%), Postives = 48/84 (57.14%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +H+A +EG V +   LL N  +++   K+    +H A+  G  +++  L++   N   +++ G+ P++ A+   HLE++K LL+
Sbjct:   61 LHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLE 144          

HSP 13 Score: 50.0618 bits (118), Expect = 2.919e-7
Identity = 28/91 (30.77%), Postives = 47/91 (51.65%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSG---WRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  +    LLL ++ +++   +    P+H A+  GH  I+K LL R  +      S      P+H A+  GH E+ + LL
Sbjct:  353 NGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNSNIFSSDYVETPLHMAARAGHCEVAQFLL 443          
BLAST of SMED30027856 vs. Ensembl Zebrafish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 80.8777 bits (198), Expect = 6.287e-18
Identity = 36/85 (42.35%), Postives = 54/85 (63.53%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            P+H+A R G  +VA+ LL NN  +D +AK    P+H A+R GH E++KLL++   N +  T +G  P+H A+  GH +  +ILLD
Sbjct:  443 PLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLD 527          

HSP 2 Score: 78.9518 bits (193), Expect = 2.852e-17
Identity = 35/88 (39.77%), Postives = 56/88 (63.64%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIA R G V + RLLLD    ID + K +  P+H A+R GH  ++++LLD+    + +TK+G  P+H A+ G H++ ++ LL
Sbjct:  241 NGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLL 328          

HSP 3 Score: 71.633 bits (174), Expect = 1.133e-14
Identity = 35/94 (37.23%), Postives = 56/94 (59.57%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            V+   G  P+HIA     + VA+LLL+   N++   K    P+H ASR G+  +++LLLDR    + +TK    P+H A+  GH+ +++ILLD+
Sbjct:  204 VLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAQIDAKTKDELTPLHCAARNGHVRVVEILLDQ 297          

HSP 4 Score: 69.707 bits (169), Expect = 5.917e-14
Identity = 32/89 (35.96%), Postives = 52/89 (58.43%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            GH P+HIA REG     R+LLD N       K+ + P+H A + G  ++++LLL+R  N     K+G  P+H A    +L+++K+L+ +
Sbjct:  506 GHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSK 594          

HSP 5 Score: 67.0106 bits (162), Expect = 4.444e-13
Identity = 39/128 (30.47%), Postives = 66/128 (51.56%), Query Frame = 1
Query:  118 GNIEMGEFECFKEQLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G+ E+ +F      L+  NA+          VD    DD   P+H A R G  ++ +LL+++  N D        P+H A+R GH++  ++LLD N      TK G+ P+H A   G ++++++LL+R
Sbjct:  451 GHCEVAQF------LLQNNAQ----------VDAKAKDD-QTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLER 561          

HSP 6 Score: 61.2326 bits (147), Expect = 4.917e-11
Identity = 30/86 (34.88%), Postives = 46/86 (53.49%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H A R G V V  +LLD    +  + K    PIH A++G H + ++ LL  N   +  T     P+H A+  GH  ++K+LLD+
Sbjct:  278 PLHCAARNGHVRVVEILLDQGAPLQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDK 363          

HSP 7 Score: 60.4622 bits (145), Expect = 9.515e-11
Identity = 40/123 (32.52%), Postives = 59/123 (47.97%), Query Frame = 1
Query:  142 ECFKEQLMNGNAEGVRENLKGEFVDKVIDD---DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +C + QL+  NAE              IDD   D   P+H+A   G   + ++LLD     +  A   + P+H A +  H   + LLL  + + E  T+SG  P+H A+  GHL I+K LL R
Sbjct:  322 DCVR-QLLQYNAE--------------IDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQR 429          

HSP 8 Score: 60.077 bits (144), Expect = 1.141e-10
Identity = 26/94 (27.66%), Postives = 51/94 (54.26%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            + + + G  P+H+A   G +++ + LL    + +    +   P+H A+R GH E+ + LL  N   + + K    P+H A+  GH E++K+L++
Sbjct:  401 EAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLME 494          

HSP 9 Score: 59.3066 bits (142), Expect = 1.806e-10
Identity = 25/90 (27.78%), Postives = 53/90 (58.89%), Query Frame = 1
Query:  226 DDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            + +G  P+H+  +EG V +A +L+    ++   ++  + P+H A   G+ +++K LL +  +   +T+ G+ P+H A+  GH +I+ +LL
Sbjct:  668 NKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLL 757          

HSP 10 Score: 57.3806 bits (137), Expect = 9.619e-10
Identity = 28/111 (25.23%), Postives = 60/111 (54.05%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +GN E   +++K      + + +G   +H+A +EG V +   LL N  +++   K+    +H A+  G  +++  L++   N   +++ G+ P++ A+   HLE++K LL+
Sbjct:   25 SGNLEKALDHIKNGIDINIANQNGLNGLHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLE 135          

HSP 11 Score: 56.6102 bits (135), Expect = 1.913e-9
Identity = 30/85 (35.29%), Postives = 49/85 (57.65%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R      A +LL N+ N D  +K  + P+H A+   +  + +LLL+R  N     K+G  P+H AS  G++ ++++LLDR
Sbjct:  180 LHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDR 264          

HSP 12 Score: 55.8398 bits (133), Expect = 3.392e-9
Identity = 31/109 (28.44%), Postives = 53/109 (48.62%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+A+  R  L        +   G  P+H+AC+ G VDV  LLL+   N +   K    P+H A    + +++KLL+ +  +     ++G+  +H A+    LE+   LL
Sbjct:  517 GHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVVELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLL 625          

HSP 13 Score: 55.0694 bits (131), Expect = 6.870e-9
Identity = 26/90 (28.89%), Postives = 50/90 (55.56%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+  +HIA ++  ++VA  LL    N + E+ +   P+H AS+ G  +++ LL+ +  N     K+G  P+H  +  GH+ I  +L+ +
Sbjct:  604 NGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQ 693          

HSP 14 Score: 54.6842 bits (130), Expect = 7.485e-9
Identity = 28/88 (31.82%), Postives = 44/88 (50.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  PIH+A +   +D  R LL  N  ID        P+H A+  GH  ++K+LLD+         +G+ P+H A    H+  + +LL
Sbjct:  307 NGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLL 394          

HSP 15 Score: 53.9138 bits (128), Expect = 1.715e-8
Identity = 25/89 (28.09%), Postives = 49/89 (55.06%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  P+H+A +EG  D+  LL+    N++   K    P+H  ++ GH  I  +L+ +  +    ++ G+ P+H A   G+++++K LL +
Sbjct:  638 GITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQ 726          

HSP 16 Score: 53.5286 bits (127), Expect = 2.075e-8
Identity = 27/88 (30.68%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  +    LLL ++ +++   +    P+H A+  GH  I+K LL R  +          P+H A+  GH E+ + LL
Sbjct:  373 NGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLL 460          

HSP 17 Score: 53.1434 bits (126), Expect = 2.896e-8
Identity = 23/86 (26.74%), Postives = 48/86 (55.81%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+H+AC  G + + + LL    +++ + +  + P+H A++ GH++I+ LLL         T +G  P+  A   G++ ++ +L
Sbjct:  704 GYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 789          
BLAST of SMED30027856 vs. Ensembl Zebrafish
Match: BX640476.1 (ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:797662])

HSP 1 Score: 80.8777 bits (198), Expect = 6.288e-18
Identity = 36/85 (42.35%), Postives = 54/85 (63.53%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            P+H+A R G  +VA+ LL NN  +D +AK    P+H A+R GH E++KLL++   N +  T +G  P+H A+  GH +  +ILLD
Sbjct:  438 PLHMAARAGHCEVAQFLLQNNAQVDAKAKDDQTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLD 522          

HSP 2 Score: 68.5514 bits (166), Expect = 1.264e-13
Identity = 32/89 (35.96%), Postives = 51/89 (57.30%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            GH P+HIA REG     R+LLD N       K+ + P+H A + G  ++ +LLL+R  N     K+G  P+H A    +L+++K+L+ +
Sbjct:  501 GHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSK 589          

HSP 3 Score: 66.2402 bits (160), Expect = 9.484e-13
Identity = 39/128 (30.47%), Postives = 65/128 (50.78%), Query Frame = 1
Query:  118 GNIEMGEFECFKEQLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G+ E+ +F      L+  NA+          VD    DD   P+H A R G  ++ +LL+++  N D        P+H A+R GH++  ++LLD N      TK G+ P+H A   G +++ ++LL+R
Sbjct:  446 GHCEVAQF------LLQNNAQ----------VDAKAKDD-QTPLHCAARMGHKELVKLLMEHKANPDSATTAGHTPLHIAAREGHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVAELLLER 556          

HSP 4 Score: 60.4622 bits (145), Expect = 9.516e-11
Identity = 40/123 (32.52%), Postives = 59/123 (47.97%), Query Frame = 1
Query:  142 ECFKEQLMNGNAEGVRENLKGEFVDKVIDD---DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +C + QL+  NAE              IDD   D   P+H+A   G   + ++LLD     +  A   + P+H A +  H   + LLL  + + E  T+SG  P+H A+  GHL I+K LL R
Sbjct:  314 DCVR-QLLQYNAE--------------IDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQR 421          

HSP 5 Score: 59.3066 bits (142), Expect = 1.806e-10
Identity = 25/90 (27.78%), Postives = 53/90 (58.89%), Query Frame = 1
Query:  226 DDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            + +G  P+H+  +EG V +A +L+    ++   ++  + P+H A   G+ +++K LL +  +   +T+ G+ P+H A+  GH +I+ +LL
Sbjct:  663 NKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLL 752          

HSP 6 Score: 57.3806 bits (137), Expect = 8.578e-10
Identity = 32/109 (29.36%), Postives = 54/109 (49.54%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+A+  R  L        +   G  P+H+AC+ G VDVA LLL+   N +   K    P+H A    + +++KLL+ +  +     ++G+  +H A+    LE+   LL
Sbjct:  512 GHAQTTRILLDENAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTALHIAAKQNQLEVASSLL 620          

HSP 7 Score: 55.0694 bits (131), Expect = 5.955e-9
Identity = 31/105 (29.52%), Postives = 49/105 (46.67%), Query Frame = 1
Query:  181 GVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G   N   +    +I  +G  PIH+A +   +D  R LL  N  ID        P+H A+  GH  ++K+LLD+         +G+ P+H A    H+  + +LL
Sbjct:  282 GANVNFTPKVSSTLILLNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLL 386          

HSP 8 Score: 55.0694 bits (131), Expect = 6.870e-9
Identity = 26/90 (28.89%), Postives = 50/90 (55.56%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+  +HIA ++  ++VA  LL    N + E+ +   P+H AS+ G  +++ LL+ +  N     K+G  P+H  +  GH+ I  +L+ +
Sbjct:  599 NGYTALHIAAKQNQLEVASSLLQYGANANSESLQGITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQ 688          

HSP 9 Score: 53.9138 bits (128), Expect = 1.715e-8
Identity = 25/89 (28.09%), Postives = 49/89 (55.06%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  P+H+A +EG  D+  LL+    N++   K    P+H  ++ GH  I  +L+ +  +    ++ G+ P+H A   G+++++K LL +
Sbjct:  633 GITPLHLASQEGQPDMVALLISKQANVNLGNKNGLTPLHLVAQEGHVGIADMLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQ 721          

HSP 10 Score: 53.1434 bits (126), Expect = 2.896e-8
Identity = 23/86 (26.74%), Postives = 48/86 (55.81%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+H+AC  G + + + LL    +++ + +  + P+H A++ GH++I+ LLL         T +G  P+  A   G++ ++ +L
Sbjct:  699 GYTPLHVACHYGNIKMVKFLLQQQAHVNSKTRLGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 784          

HSP 11 Score: 52.7582 bits (125), Expect = 3.965e-8
Identity = 31/97 (31.96%), Postives = 49/97 (50.52%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAK--------RKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  P+HIA     + VA+LLL+   N++   K            PIH A++G H + ++ LL  N   +  T     P+H A+  GH  ++K+LLD+
Sbjct:  259 GFTPLHIAAHYENLSVAQLLLNRGANVNFTPKVSSTLILLNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMVKVLLDK 355          

HSP 12 Score: 52.7582 bits (125), Expect = 4.238e-8
Identity = 23/84 (27.38%), Postives = 48/84 (57.14%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +H+A +EG V +   LL N  +++   K+    +H A+  G  +++  L++   N   +++ G+ P++ A+   HLE++K LL+
Sbjct:   73 LHLASKEGHVKMVLELLHNGIDLETTTKKGNTALHIAALAGQEKVVAELINYGANVNAQSQKGFSPLYMAAQENHLEVVKYLLE 156          

HSP 13 Score: 50.0618 bits (118), Expect = 2.920e-7
Identity = 28/91 (30.77%), Postives = 47/91 (51.65%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSG---WRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  +    LLL ++ +++   +    P+H A+  GH  I+K LL R  +      S      P+H A+  GH E+ + LL
Sbjct:  365 NGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNSNIFSSDYVETPLHMAARAGHCEVAQFLL 455          
BLAST of SMED30027856 vs. Ensembl Xenopus
Match: ANK2 (ankyrin 2, neuronal [Source:NCBI gene;Acc:100494806])

HSP 1 Score: 76.6406 bits (187), Expect = 3.463e-16
Identity = 35/91 (38.46%), Postives = 58/91 (63.74%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            + G  P+HIA   G V+VA LLL+    +D  A+    P+H AS+ G++ ++KLLLDR    + +T+ G  P+H A+  GH +++++LL+R
Sbjct:  210 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLER 300          

HSP 2 Score: 74.3294 bits (181), Expect = 2.054e-15
Identity = 33/88 (37.50%), Postives = 54/88 (61.36%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A + G  ++ +LLLD    ID + +    P+H A+R GH ++++LLL+R       TK+G  P+H A+ G H+E +K LL
Sbjct:  244 NGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLL 331          

HSP 3 Score: 66.6254 bits (161), Expect = 1.018e-12
Identity = 31/88 (35.23%), Postives = 53/88 (60.23%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G   +H+A R G V+V R LL N   +D  A+ +  P+H ASR G +EI++LLL    + +  T +G+ P+H ++  G +++  +LL+
Sbjct:  443 GETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLE 530          

HSP 4 Score: 65.4698 bits (158), Expect = 2.012e-12
Identity = 30/111 (27.03%), Postives = 64/111 (57.66%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +GN + V E LKG       + +G   +H+A +EG + + + L++    +D   K+    +H AS  G +E++K+L+ +  N   ++++G+ P++ A+   H++++K LL+
Sbjct:   20 SGNLDKVVEYLKGGIDINTSNQNGLNALHLAAKEGHIGLVQELMERGSAVDSATKKGNTALHIASLAGQAEVVKILVKQGANINAQSQNGFTPLYMAAQENHIDVVKYLLE 130          

HSP 5 Score: 62.3882 bits (150), Expect = 2.861e-11
Identity = 29/88 (32.95%), Postives = 50/88 (56.82%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +E  V V  +L  N  N+D + K  + P+  A   G+ +++  LL+   N   +TK+G+ P+H A+  GH  I+ +LL
Sbjct:  673 NGLTPLHLAAQEDRVIVGEILSKNGANLDAQTKLGYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLL 760          

HSP 6 Score: 61.6178 bits (148), Expect = 5.280e-11
Identity = 36/112 (32.14%), Postives = 59/112 (52.68%), Query Frame = 1
Query:  169 GNAEGVRENLK-GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  E VR  L+ G  VD    ++   P+HIA R G  ++ +LLL +  + D      + P+H ++R G  ++  +LL+        TK G+ P+H A+  G L++ K+LL R
Sbjct:  454 GQVEVVRCLLRNGALVDARAREE-QTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQR 564          

HSP 7 Score: 61.2326 bits (147), Expect = 5.811e-11
Identity = 31/90 (34.44%), Postives = 53/90 (58.89%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+ P+HI+ REG VDVA +LL+         K+ + P+H A++ G  ++ KLLL R    +   K+G  P+H A+   + ++  +LLD+
Sbjct:  508 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDK 597          

HSP 8 Score: 59.3066 bits (142), Expect = 3.100e-10
Identity = 29/88 (32.95%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A + G +DVA+LLL      D   K    P+H A+   + ++  LLLD+  + +   K+G+ P+H AS    ++I   LL+
Sbjct:  542 GFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIATTLLN 629          

HSP 9 Score: 55.0694 bits (131), Expect = 1.002e-8
Identity = 28/100 (28.00%), Postives = 55/100 (55.00%), Query Frame = 1
Query:  199 KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +G  VD      G+  +HIA   G  +V ++L+    NI+ +++  + P++ A++  H +++K LL+   N    T+ G+ P+  A   GH +++ ILL+
Sbjct:   65 RGSAVDSATKK-GNTALHIASLAGQAEVVKILVKQGANINAQSQNGFTPLYMAAQENHIDVVKYLLETGANQSTATEDGFTPLAVALQQGHNQVVAILLE 163          

HSP 10 Score: 54.6842 bits (130), Expect = 1.212e-8
Identity = 31/93 (33.33%), Postives = 50/93 (53.76%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAK--------RKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R+     A LLL N+ N D ++K          + P+H A+  G+  +  LLL+R    +   ++G  P+H AS  G+  ++K+LLDR
Sbjct:  175 LHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDR 267          

HSP 11 Score: 54.6842 bits (130), Expect = 1.271e-8
Identity = 26/92 (28.26%), Postives = 52/92 (56.52%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            +V   +G+ P+HIA ++  + +A  LL+     +    +   P+H A++ GH++++ LLL++  N    TK+G  P+H A+    + + +IL
Sbjct:  602 QVTAKNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNKQANIHVGTKNGLTPLHLAAQEDRVIVGEIL 693          

HSP 12 Score: 54.299 bits (129), Expect = 1.553e-8
Identity = 27/83 (32.53%), Postives = 46/83 (55.42%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A   G   V +LLLD   N +  A   + P+H A +    ++++LL+    + +  T+SG  P+H A+  GHL I+ +LL
Sbjct:  348 LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL 430          

HSP 13 Score: 52.373 bits (124), Expect = 6.602e-8
Identity = 28/90 (31.11%), Postives = 45/90 (50.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A       VA LLLD   +    AK  + P+H AS+    +I   LL+        T  G  P+H A+  GH +++ +LL++
Sbjct:  574 NGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNK 663          

HSP 14 Score: 51.2174 bits (121), Expect = 1.879e-7
Identity = 27/88 (30.68%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LL+    +I    +    PIH A+  GH  I+ LLL    + +     G   +H A+  G +E+++ LL
Sbjct:  376 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLL 463          

HSP 15 Score: 48.521 bits (114), Expect = 1.393e-6
Identity = 22/86 (25.58%), Postives = 45/86 (52.33%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+ +AC  G + +   LL++  N++ + K  + P+H A++ GH+ I+ +LL         T +G   +  A   G++ ++  L
Sbjct:  707 GYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQNGAKPNVTTSNGNTALAIARRLGYISVVDTL 792          

HSP 16 Score: 46.2098 bits (108), Expect = 9.696e-6
Identity = 23/88 (26.14%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +   V+  + LL +   +D         +H A+  GH  + KLLLD+  N      +G+ P+H A     ++++++L+
Sbjct:  310 NGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTSLHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLV 397          
BLAST of SMED30027856 vs. Ensembl Xenopus
Match: ANK2 (ankyrin 2, neuronal [Source:NCBI gene;Acc:100494806])

HSP 1 Score: 76.6406 bits (187), Expect = 3.464e-16
Identity = 35/91 (38.46%), Postives = 58/91 (63.74%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            + G  P+HIA   G V+VA LLL+    +D  A+    P+H AS+ G++ ++KLLLDR    + +T+ G  P+H A+  GH +++++LL+R
Sbjct:  231 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLER 321          

HSP 2 Score: 74.3294 bits (181), Expect = 2.054e-15
Identity = 33/88 (37.50%), Postives = 54/88 (61.36%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A + G  ++ +LLLD    ID + +    P+H A+R GH ++++LLL+R       TK+G  P+H A+ G H+E +K LL
Sbjct:  265 NGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLL 352          

HSP 3 Score: 66.6254 bits (161), Expect = 1.018e-12
Identity = 31/88 (35.23%), Postives = 53/88 (60.23%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G   +H+A R G V+V R LL N   +D  A+ +  P+H ASR G +EI++LLL    + +  T +G+ P+H ++  G +++  +LL+
Sbjct:  464 GETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLE 551          

HSP 4 Score: 65.4698 bits (158), Expect = 2.012e-12
Identity = 30/111 (27.03%), Postives = 64/111 (57.66%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +GN + V E LKG       + +G   +H+A +EG + + + L++    +D   K+    +H AS  G +E++K+L+ +  N   ++++G+ P++ A+   H++++K LL+
Sbjct:   41 SGNLDKVVEYLKGGIDINTSNQNGLNALHLAAKEGHIGLVQELMERGSAVDSATKKGNTALHIASLAGQAEVVKILVKQGANINAQSQNGFTPLYMAAQENHIDVVKYLLE 151          

HSP 5 Score: 62.3882 bits (150), Expect = 2.862e-11
Identity = 29/88 (32.95%), Postives = 50/88 (56.82%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +E  V V  +L  N  N+D + K  + P+  A   G+ +++  LL+   N   +TK+G+ P+H A+  GH  I+ +LL
Sbjct:  694 NGLTPLHLAAQEDRVIVGEILSKNGANLDAQTKLGYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLL 781          

HSP 6 Score: 61.6178 bits (148), Expect = 5.282e-11
Identity = 36/112 (32.14%), Postives = 59/112 (52.68%), Query Frame = 1
Query:  169 GNAEGVRENLK-GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  E VR  L+ G  VD    ++   P+HIA R G  ++ +LLL +  + D      + P+H ++R G  ++  +LL+        TK G+ P+H A+  G L++ K+LL R
Sbjct:  475 GQVEVVRCLLRNGALVDARAREE-QTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQR 585          

HSP 7 Score: 61.2326 bits (147), Expect = 5.812e-11
Identity = 31/90 (34.44%), Postives = 53/90 (58.89%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+ P+HI+ REG VDVA +LL+         K+ + P+H A++ G  ++ KLLL R    +   K+G  P+H A+   + ++  +LLD+
Sbjct:  529 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDK 618          

HSP 8 Score: 59.3066 bits (142), Expect = 3.100e-10
Identity = 29/88 (32.95%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A + G +DVA+LLL      D   K    P+H A+   + ++  LLLD+  + +   K+G+ P+H AS    ++I   LL+
Sbjct:  563 GFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIATTLLN 650          

HSP 9 Score: 55.0694 bits (131), Expect = 1.002e-8
Identity = 28/100 (28.00%), Postives = 55/100 (55.00%), Query Frame = 1
Query:  199 KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +G  VD      G+  +HIA   G  +V ++L+    NI+ +++  + P++ A++  H +++K LL+   N    T+ G+ P+  A   GH +++ ILL+
Sbjct:   86 RGSAVDSATKK-GNTALHIASLAGQAEVVKILVKQGANINAQSQNGFTPLYMAAQENHIDVVKYLLETGANQSTATEDGFTPLAVALQQGHNQVVAILLE 184          

HSP 10 Score: 54.6842 bits (130), Expect = 1.212e-8
Identity = 31/93 (33.33%), Postives = 50/93 (53.76%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAK--------RKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R+     A LLL N+ N D ++K          + P+H A+  G+  +  LLL+R    +   ++G  P+H AS  G+  ++K+LLDR
Sbjct:  196 LHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDR 288          

HSP 11 Score: 54.6842 bits (130), Expect = 1.271e-8
Identity = 26/92 (28.26%), Postives = 52/92 (56.52%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            +V   +G+ P+HIA ++  + +A  LL+     +    +   P+H A++ GH++++ LLL++  N    TK+G  P+H A+    + + +IL
Sbjct:  623 QVTAKNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNKQANIHVGTKNGLTPLHLAAQEDRVIVGEIL 714          

HSP 12 Score: 54.299 bits (129), Expect = 1.553e-8
Identity = 27/83 (32.53%), Postives = 46/83 (55.42%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A   G   V +LLLD   N +  A   + P+H A +    ++++LL+    + +  T+SG  P+H A+  GHL I+ +LL
Sbjct:  369 LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL 451          

HSP 13 Score: 52.373 bits (124), Expect = 6.604e-8
Identity = 28/90 (31.11%), Postives = 45/90 (50.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A       VA LLLD   +    AK  + P+H AS+    +I   LL+        T  G  P+H A+  GH +++ +LL++
Sbjct:  595 NGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNK 684          

HSP 14 Score: 51.2174 bits (121), Expect = 1.880e-7
Identity = 27/88 (30.68%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LL+    +I    +    PIH A+  GH  I+ LLL    + +     G   +H A+  G +E+++ LL
Sbjct:  397 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLL 484          

HSP 15 Score: 48.521 bits (114), Expect = 1.393e-6
Identity = 22/86 (25.58%), Postives = 45/86 (52.33%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+ +AC  G + +   LL++  N++ + K  + P+H A++ GH+ I+ +LL         T +G   +  A   G++ ++  L
Sbjct:  728 GYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQNGAKPNVTTSNGNTALAIARRLGYISVVDTL 813          

HSP 16 Score: 46.2098 bits (108), Expect = 9.698e-6
Identity = 23/88 (26.14%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +   V+  + LL +   +D         +H A+  GH  + KLLLD+  N      +G+ P+H A     ++++++L+
Sbjct:  331 NGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTSLHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLV 418          
BLAST of SMED30027856 vs. Ensembl Xenopus
Match: ANK2 (ankyrin 2, neuronal [Source:NCBI gene;Acc:100494806])

HSP 1 Score: 76.6406 bits (187), Expect = 3.465e-16
Identity = 35/91 (38.46%), Postives = 58/91 (63.74%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            + G  P+HIA   G V+VA LLL+    +D  A+    P+H AS+ G++ ++KLLLDR    + +T+ G  P+H A+  GH +++++LL+R
Sbjct:  210 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLER 300          

HSP 2 Score: 74.3294 bits (181), Expect = 2.054e-15
Identity = 33/88 (37.50%), Postives = 54/88 (61.36%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A + G  ++ +LLLD    ID + +    P+H A+R GH ++++LLL+R       TK+G  P+H A+ G H+E +K LL
Sbjct:  244 NGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLL 331          

HSP 3 Score: 66.6254 bits (161), Expect = 1.019e-12
Identity = 31/88 (35.23%), Postives = 53/88 (60.23%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G   +H+A R G V+V R LL N   +D  A+ +  P+H ASR G +EI++LLL    + +  T +G+ P+H ++  G +++  +LL+
Sbjct:  443 GETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLE 530          

HSP 4 Score: 65.4698 bits (158), Expect = 2.013e-12
Identity = 30/111 (27.03%), Postives = 64/111 (57.66%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +GN + V E LKG       + +G   +H+A +EG + + + L++    +D   K+    +H AS  G +E++K+L+ +  N   ++++G+ P++ A+   H++++K LL+
Sbjct:   20 SGNLDKVVEYLKGGIDINTSNQNGLNALHLAAKEGHIGLVQELMERGSAVDSATKKGNTALHIASLAGQAEVVKILVKQGANINAQSQNGFTPLYMAAQENHIDVVKYLLE 130          

HSP 5 Score: 62.3882 bits (150), Expect = 2.862e-11
Identity = 29/88 (32.95%), Postives = 50/88 (56.82%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +E  V V  +L  N  N+D + K  + P+  A   G+ +++  LL+   N   +TK+G+ P+H A+  GH  I+ +LL
Sbjct:  673 NGLTPLHLAAQEDRVIVGEILSKNGANLDAQTKLGYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLL 760          

HSP 6 Score: 61.6178 bits (148), Expect = 5.282e-11
Identity = 36/112 (32.14%), Postives = 59/112 (52.68%), Query Frame = 1
Query:  169 GNAEGVRENLK-GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  E VR  L+ G  VD    ++   P+HIA R G  ++ +LLL +  + D      + P+H ++R G  ++  +LL+        TK G+ P+H A+  G L++ K+LL R
Sbjct:  454 GQVEVVRCLLRNGALVDARAREE-QTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQR 564          

HSP 7 Score: 61.2326 bits (147), Expect = 5.813e-11
Identity = 31/90 (34.44%), Postives = 53/90 (58.89%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+ P+HI+ REG VDVA +LL+         K+ + P+H A++ G  ++ KLLL R    +   K+G  P+H A+   + ++  +LLD+
Sbjct:  508 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDK 597          

HSP 8 Score: 59.3066 bits (142), Expect = 3.101e-10
Identity = 29/88 (32.95%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A + G +DVA+LLL      D   K    P+H A+   + ++  LLLD+  + +   K+G+ P+H AS    ++I   LL+
Sbjct:  542 GFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIATTLLN 629          

HSP 9 Score: 55.0694 bits (131), Expect = 1.002e-8
Identity = 28/100 (28.00%), Postives = 55/100 (55.00%), Query Frame = 1
Query:  199 KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +G  VD      G+  +HIA   G  +V ++L+    NI+ +++  + P++ A++  H +++K LL+   N    T+ G+ P+  A   GH +++ ILL+
Sbjct:   65 RGSAVDSATKK-GNTALHIASLAGQAEVVKILVKQGANINAQSQNGFTPLYMAAQENHIDVVKYLLETGANQSTATEDGFTPLAVALQQGHNQVVAILLE 163          

HSP 10 Score: 54.6842 bits (130), Expect = 1.212e-8
Identity = 31/93 (33.33%), Postives = 50/93 (53.76%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAK--------RKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R+     A LLL N+ N D ++K          + P+H A+  G+  +  LLL+R    +   ++G  P+H AS  G+  ++K+LLDR
Sbjct:  175 LHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDR 267          

HSP 11 Score: 54.6842 bits (130), Expect = 1.272e-8
Identity = 26/92 (28.26%), Postives = 52/92 (56.52%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            +V   +G+ P+HIA ++  + +A  LL+     +    +   P+H A++ GH++++ LLL++  N    TK+G  P+H A+    + + +IL
Sbjct:  602 QVTAKNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNKQANIHVGTKNGLTPLHLAAQEDRVIVGEIL 693          

HSP 12 Score: 54.299 bits (129), Expect = 1.553e-8
Identity = 27/83 (32.53%), Postives = 46/83 (55.42%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A   G   V +LLLD   N +  A   + P+H A +    ++++LL+    + +  T+SG  P+H A+  GHL I+ +LL
Sbjct:  348 LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL 430          

HSP 13 Score: 52.373 bits (124), Expect = 6.605e-8
Identity = 28/90 (31.11%), Postives = 45/90 (50.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A       VA LLLD   +    AK  + P+H AS+    +I   LL+        T  G  P+H A+  GH +++ +LL++
Sbjct:  574 NGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNK 663          

HSP 14 Score: 51.2174 bits (121), Expect = 1.880e-7
Identity = 27/88 (30.68%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LL+    +I    +    PIH A+  GH  I+ LLL    + +     G   +H A+  G +E+++ LL
Sbjct:  376 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLL 463          

HSP 15 Score: 48.521 bits (114), Expect = 1.393e-6
Identity = 22/86 (25.58%), Postives = 45/86 (52.33%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+ +AC  G + +   LL++  N++ + K  + P+H A++ GH+ I+ +LL         T +G   +  A   G++ ++  L
Sbjct:  707 GYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQNGAKPNVTTSNGNTALAIARRLGYISVVDTL 792          

HSP 16 Score: 46.2098 bits (108), Expect = 9.700e-6
Identity = 23/88 (26.14%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +   V+  + LL +   +D         +H A+  GH  + KLLLD+  N      +G+ P+H A     ++++++L+
Sbjct:  310 NGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTSLHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLV 397          
BLAST of SMED30027856 vs. Ensembl Xenopus
Match: ANK2 (ankyrin 2, neuronal [Source:NCBI gene;Acc:100494806])

HSP 1 Score: 76.6406 bits (187), Expect = 3.481e-16
Identity = 35/91 (38.46%), Postives = 58/91 (63.74%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            + G  P+HIA   G V+VA LLL+    +D  A+    P+H AS+ G++ ++KLLLDR    + +T+ G  P+H A+  GH +++++LL+R
Sbjct:  210 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLER 300          

HSP 2 Score: 74.3294 bits (181), Expect = 2.064e-15
Identity = 33/88 (37.50%), Postives = 54/88 (61.36%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A + G  ++ +LLLD    ID + +    P+H A+R GH ++++LLL+R       TK+G  P+H A+ G H+E +K LL
Sbjct:  244 NGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLL 331          

HSP 3 Score: 66.6254 bits (161), Expect = 1.023e-12
Identity = 31/88 (35.23%), Postives = 53/88 (60.23%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G   +H+A R G V+V R LL N   +D  A+ +  P+H ASR G +EI++LLL    + +  T +G+ P+H ++  G +++  +LL+
Sbjct:  443 GETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLE 530          

HSP 4 Score: 65.4698 bits (158), Expect = 2.022e-12
Identity = 30/111 (27.03%), Postives = 64/111 (57.66%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +GN + V E LKG       + +G   +H+A +EG + + + L++    +D   K+    +H AS  G +E++K+L+ +  N   ++++G+ P++ A+   H++++K LL+
Sbjct:   20 SGNLDKVVEYLKGGIDINTSNQNGLNALHLAAKEGHIGLVQELMERGSAVDSATKKGNTALHIASLAGQAEVVKILVKQGANINAQSQNGFTPLYMAAQENHIDVVKYLLE 130          

HSP 5 Score: 62.3882 bits (150), Expect = 2.875e-11
Identity = 29/88 (32.95%), Postives = 50/88 (56.82%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +E  V V  +L  N  N+D + K  + P+  A   G+ +++  LL+   N   +TK+G+ P+H A+  GH  I+ +LL
Sbjct:  673 NGLTPLHLAAQEDRVIVGEILSKNGANLDAQTKLGYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLL 760          

HSP 6 Score: 61.6178 bits (148), Expect = 5.307e-11
Identity = 36/112 (32.14%), Postives = 59/112 (52.68%), Query Frame = 1
Query:  169 GNAEGVRENLK-GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  E VR  L+ G  VD    ++   P+HIA R G  ++ +LLL +  + D      + P+H ++R G  ++  +LL+        TK G+ P+H A+  G L++ K+LL R
Sbjct:  454 GQVEVVRCLLRNGALVDARAREE-QTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQR 564          

HSP 7 Score: 61.2326 bits (147), Expect = 5.840e-11
Identity = 31/90 (34.44%), Postives = 53/90 (58.89%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+ P+HI+ REG VDVA +LL+         K+ + P+H A++ G  ++ KLLL R    +   K+G  P+H A+   + ++  +LLD+
Sbjct:  508 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDK 597          

HSP 8 Score: 59.3066 bits (142), Expect = 3.115e-10
Identity = 29/88 (32.95%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A + G +DVA+LLL      D   K    P+H A+   + ++  LLLD+  + +   K+G+ P+H AS    ++I   LL+
Sbjct:  542 GFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIATTLLN 629          

HSP 9 Score: 55.0694 bits (131), Expect = 1.006e-8
Identity = 28/100 (28.00%), Postives = 55/100 (55.00%), Query Frame = 1
Query:  199 KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +G  VD      G+  +HIA   G  +V ++L+    NI+ +++  + P++ A++  H +++K LL+   N    T+ G+ P+  A   GH +++ ILL+
Sbjct:   65 RGSAVDSATKK-GNTALHIASLAGQAEVVKILVKQGANINAQSQNGFTPLYMAAQENHIDVVKYLLETGANQSTATEDGFTPLAVALQQGHNQVVAILLE 163          

HSP 10 Score: 54.6842 bits (130), Expect = 1.218e-8
Identity = 31/93 (33.33%), Postives = 50/93 (53.76%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAK--------RKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R+     A LLL N+ N D ++K          + P+H A+  G+  +  LLL+R    +   ++G  P+H AS  G+  ++K+LLDR
Sbjct:  175 LHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDR 267          

HSP 11 Score: 54.6842 bits (130), Expect = 1.277e-8
Identity = 26/92 (28.26%), Postives = 52/92 (56.52%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            +V   +G+ P+HIA ++  + +A  LL+     +    +   P+H A++ GH++++ LLL++  N    TK+G  P+H A+    + + +IL
Sbjct:  602 QVTAKNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNKQANIHVGTKNGLTPLHLAAQEDRVIVGEIL 693          

HSP 12 Score: 54.299 bits (129), Expect = 1.560e-8
Identity = 27/83 (32.53%), Postives = 46/83 (55.42%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A   G   V +LLLD   N +  A   + P+H A +    ++++LL+    + +  T+SG  P+H A+  GHL I+ +LL
Sbjct:  348 LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL 430          

HSP 13 Score: 52.373 bits (124), Expect = 6.634e-8
Identity = 28/90 (31.11%), Postives = 45/90 (50.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A       VA LLLD   +    AK  + P+H AS+    +I   LL+        T  G  P+H A+  GH +++ +LL++
Sbjct:  574 NGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNK 663          

HSP 14 Score: 51.2174 bits (121), Expect = 1.888e-7
Identity = 27/88 (30.68%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LL+    +I    +    PIH A+  GH  I+ LLL    + +     G   +H A+  G +E+++ LL
Sbjct:  376 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLL 463          

HSP 15 Score: 48.521 bits (114), Expect = 1.399e-6
Identity = 22/86 (25.58%), Postives = 45/86 (52.33%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+ +AC  G + +   LL++  N++ + K  + P+H A++ GH+ I+ +LL         T +G   +  A   G++ ++  L
Sbjct:  707 GYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQNGAKPNVTTSNGNTALAIARRLGYISVVDTL 792          

HSP 16 Score: 46.2098 bits (108), Expect = 9.741e-6
Identity = 23/88 (26.14%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +   V+  + LL +   +D         +H A+  GH  + KLLLD+  N      +G+ P+H A     ++++++L+
Sbjct:  310 NGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTSLHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLV 397          
BLAST of SMED30027856 vs. Ensembl Xenopus
Match: ANK2 (ankyrin 2, neuronal [Source:NCBI gene;Acc:100494806])

HSP 1 Score: 76.6406 bits (187), Expect = 3.481e-16
Identity = 35/91 (38.46%), Postives = 58/91 (63.74%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            + G  P+HIA   G V+VA LLL+    +D  A+    P+H AS+ G++ ++KLLLDR    + +T+ G  P+H A+  GH +++++LL+R
Sbjct:  227 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLER 317          

HSP 2 Score: 74.3294 bits (181), Expect = 2.064e-15
Identity = 33/88 (37.50%), Postives = 54/88 (61.36%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A + G  ++ +LLLD    ID + +    P+H A+R GH ++++LLL+R       TK+G  P+H A+ G H+E +K LL
Sbjct:  261 NGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLL 348          

HSP 3 Score: 66.6254 bits (161), Expect = 1.023e-12
Identity = 31/88 (35.23%), Postives = 53/88 (60.23%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G   +H+A R G V+V R LL N   +D  A+ +  P+H ASR G +EI++LLL    + +  T +G+ P+H ++  G +++  +LL+
Sbjct:  460 GETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLE 547          

HSP 4 Score: 65.4698 bits (158), Expect = 2.022e-12
Identity = 30/111 (27.03%), Postives = 64/111 (57.66%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +GN + V E LKG       + +G   +H+A +EG + + + L++    +D   K+    +H AS  G +E++K+L+ +  N   ++++G+ P++ A+   H++++K LL+
Sbjct:   37 SGNLDKVVEYLKGGIDINTSNQNGLNALHLAAKEGHIGLVQELMERGSAVDSATKKGNTALHIASLAGQAEVVKILVKQGANINAQSQNGFTPLYMAAQENHIDVVKYLLE 147          

HSP 5 Score: 62.3882 bits (150), Expect = 2.876e-11
Identity = 29/88 (32.95%), Postives = 50/88 (56.82%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +E  V V  +L  N  N+D + K  + P+  A   G+ +++  LL+   N   +TK+G+ P+H A+  GH  I+ +LL
Sbjct:  690 NGLTPLHLAAQEDRVIVGEILSKNGANLDAQTKLGYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLL 777          

HSP 6 Score: 61.6178 bits (148), Expect = 5.307e-11
Identity = 36/112 (32.14%), Postives = 59/112 (52.68%), Query Frame = 1
Query:  169 GNAEGVRENLK-GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  E VR  L+ G  VD    ++   P+HIA R G  ++ +LLL +  + D      + P+H ++R G  ++  +LL+        TK G+ P+H A+  G L++ K+LL R
Sbjct:  471 GQVEVVRCLLRNGALVDARAREE-QTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQR 581          

HSP 7 Score: 61.2326 bits (147), Expect = 5.840e-11
Identity = 31/90 (34.44%), Postives = 53/90 (58.89%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+ P+HI+ REG VDVA +LL+         K+ + P+H A++ G  ++ KLLL R    +   K+G  P+H A+   + ++  +LLD+
Sbjct:  525 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDK 614          

HSP 8 Score: 59.3066 bits (142), Expect = 3.115e-10
Identity = 29/88 (32.95%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A + G +DVA+LLL      D   K    P+H A+   + ++  LLLD+  + +   K+G+ P+H AS    ++I   LL+
Sbjct:  559 GFTPLHVAAKYGSLDVAKLLLQRRAPPDAAGKNGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIATTLLN 646          

HSP 9 Score: 55.0694 bits (131), Expect = 1.006e-8
Identity = 28/100 (28.00%), Postives = 55/100 (55.00%), Query Frame = 1
Query:  199 KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +G  VD      G+  +HIA   G  +V ++L+    NI+ +++  + P++ A++  H +++K LL+   N    T+ G+ P+  A   GH +++ ILL+
Sbjct:   82 RGSAVDSATKK-GNTALHIASLAGQAEVVKILVKQGANINAQSQNGFTPLYMAAQENHIDVVKYLLETGANQSTATEDGFTPLAVALQQGHNQVVAILLE 180          

HSP 10 Score: 54.6842 bits (130), Expect = 1.218e-8
Identity = 31/93 (33.33%), Postives = 50/93 (53.76%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAK--------RKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R+     A LLL N+ N D ++K          + P+H A+  G+  +  LLL+R    +   ++G  P+H AS  G+  ++K+LLDR
Sbjct:  192 LHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDR 284          

HSP 11 Score: 54.6842 bits (130), Expect = 1.277e-8
Identity = 26/92 (28.26%), Postives = 52/92 (56.52%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            +V   +G+ P+HIA ++  + +A  LL+     +    +   P+H A++ GH++++ LLL++  N    TK+G  P+H A+    + + +IL
Sbjct:  619 QVTAKNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNKQANIHVGTKNGLTPLHLAAQEDRVIVGEIL 710          

HSP 12 Score: 54.299 bits (129), Expect = 1.560e-8
Identity = 27/83 (32.53%), Postives = 46/83 (55.42%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A   G   V +LLLD   N +  A   + P+H A +    ++++LL+    + +  T+SG  P+H A+  GHL I+ +LL
Sbjct:  365 LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL 447          

HSP 13 Score: 52.373 bits (124), Expect = 6.634e-8
Identity = 28/90 (31.11%), Postives = 45/90 (50.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A       VA LLLD   +    AK  + P+H AS+    +I   LL+        T  G  P+H A+  GH +++ +LL++
Sbjct:  591 NGLTPLHVAAHYDNQKVALLLLDKGASPQVTAKNGYTPLHIASKKNQMQIATTLLNYGAETNILTNQGVTPLHLAAQEGHADMVTLLLNK 680          

HSP 14 Score: 51.2174 bits (121), Expect = 1.888e-7
Identity = 27/88 (30.68%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LL+    +I    +    PIH A+  GH  I+ LLL    + +     G   +H A+  G +E+++ LL
Sbjct:  393 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLL 480          

HSP 15 Score: 48.521 bits (114), Expect = 1.399e-6
Identity = 22/86 (25.58%), Postives = 45/86 (52.33%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+ +AC  G + +   LL++  N++ + K  + P+H A++ GH+ I+ +LL         T +G   +  A   G++ ++  L
Sbjct:  724 GYSPLIVACHYGNIKMVNFLLNHGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQNGAKPNVTTSNGNTALAIARRLGYISVVDTL 809          

HSP 16 Score: 46.2098 bits (108), Expect = 9.741e-6
Identity = 23/88 (26.14%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +   V+  + LL +   +D         +H A+  GH  + KLLLD+  N      +G+ P+H A     ++++++L+
Sbjct:  327 NGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTSLHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLV 414          
BLAST of SMED30027856 vs. Ensembl Mouse
Match: Ank2 (ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025])

HSP 1 Score: 78.5666 bits (192), Expect = 3.146e-17
Identity = 35/91 (38.46%), Postives = 58/91 (63.74%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            + G  P+HIA   G V+VA LLL+    +D  A+    P+H AS+ G++ ++KLLLDR    + +T+ G  P+H A+  GH +++++LL+R
Sbjct:  210 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLER 300          

HSP 2 Score: 75.485 bits (184), Expect = 3.876e-16
Identity = 33/88 (37.50%), Postives = 54/88 (61.36%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A + G  ++ +LLLD    ID + +    P+H A+R GH ++++LLL+R       TK+G  P+H A+ G H+E +K LL
Sbjct:  244 NGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLL 331          

HSP 3 Score: 68.5514 bits (166), Expect = 1.284e-13
Identity = 32/110 (29.09%), Postives = 62/110 (56.36%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            GN + V E LKG       + +G   +H+A +EG V + + LL    ++D   K+    +H AS  G +E++K+L+    N   ++++G+ P++ A+   H++++K LL+
Sbjct:   21 GNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLE 130          

HSP 4 Score: 58.151 bits (139), Expect = 3.977e-10
Identity = 28/100 (28.00%), Postives = 54/100 (54.00%), Query Frame = 1
Query:  199 KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +G  VD      G+  +HIA   G  +V ++L+    NI+ +++  + P++ A++  H +++K LL+   N    T+ G+ P+  A   GH + + ILL+
Sbjct:   65 RGSSVDSATKK-GNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLE 163          

HSP 5 Score: 57.7658 bits (138), Expect = 6.177e-10
Identity = 31/93 (33.33%), Postives = 50/93 (53.76%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAK--------RKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R+     A LLL N+ N D ++K          + P+H A+  G+  +  LLL+R    +   ++G  P+H AS  G+  ++K+LLDR
Sbjct:  175 LHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDR 267          
BLAST of SMED30027856 vs. Ensembl Mouse
Match: Ank2 (ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025])

HSP 1 Score: 78.5666 bits (192), Expect = 3.409e-17
Identity = 35/91 (38.46%), Postives = 58/91 (63.74%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            + G  P+HIA   G V+VA LLL+    +D  A+    P+H AS+ G++ ++KLLLDR    + +T+ G  P+H A+  GH +++++LL+R
Sbjct:  243 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLER 333          

HSP 2 Score: 75.8702 bits (185), Expect = 4.008e-16
Identity = 33/88 (37.50%), Postives = 54/88 (61.36%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A + G  ++ +LLLD    ID + +    P+H A+R GH ++++LLL+R       TK+G  P+H A+ G H+E +K LL
Sbjct:  277 NGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLL 364          

HSP 3 Score: 68.5514 bits (166), Expect = 1.387e-13
Identity = 32/110 (29.09%), Postives = 62/110 (56.36%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            GN + V E LKG       + +G   +H+A +EG V + + LL    ++D   K+    +H AS  G +E++K+L+    N   ++++G+ P++ A+   H++++K LL+
Sbjct:   54 GNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLE 163          

HSP 4 Score: 58.5362 bits (140), Expect = 3.644e-10
Identity = 28/100 (28.00%), Postives = 54/100 (54.00%), Query Frame = 1
Query:  199 KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +G  VD      G+  +HIA   G  +V ++L+    NI+ +++  + P++ A++  H +++K LL+   N    T+ G+ P+  A   GH + + ILL+
Sbjct:   98 RGSSVDSATKK-GNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLE 196          

HSP 5 Score: 57.7658 bits (138), Expect = 5.879e-10
Identity = 31/93 (33.33%), Postives = 50/93 (53.76%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAK--------RKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R+     A LLL N+ N D ++K          + P+H A+  G+  +  LLL+R    +   ++G  P+H AS  G+  ++K+LLDR
Sbjct:  208 LHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDR 300          

HSP 6 Score: 56.6102 bits (135), Expect = 1.682e-9
Identity = 27/83 (32.53%), Postives = 46/83 (55.42%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A   G   V +LLLD   N +  A   + P+H A +    ++++LL+    + +  T+SG  P+H A+  GHL I+ +LL
Sbjct:  381 LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL 463          

HSP 7 Score: 46.9802 bits (110), Expect = 3.229e-6
Identity = 29/121 (23.97%), Postives = 47/121 (38.84%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRG---------------------------------GHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            DG  P+H A R G   V  LLL+    +    K    P+H A++G                                 GH  + KLLLD+  N      +G+ P+H A     ++++++L+
Sbjct:  310 DGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLV 430          
BLAST of SMED30027856 vs. Ensembl Mouse
Match: Ank2 (ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025])

HSP 1 Score: 78.5666 bits (192), Expect = 4.059e-17
Identity = 36/94 (38.30%), Postives = 58/94 (61.70%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            V    G  P+HIA   G V+VA LLL+    +D  A+    P+H AS+ G++ ++KLLLDR    + +T+ G  P+H A+  GH +++++LL+R
Sbjct:  232 VQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLER 325          

HSP 2 Score: 75.485 bits (184), Expect = 4.297e-16
Identity = 33/88 (37.50%), Postives = 54/88 (61.36%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A + G  ++ +LLLD    ID + +    P+H A+R GH ++++LLL+R       TK+G  P+H A+ G H+E +K LL
Sbjct:  269 NGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLL 356          

HSP 3 Score: 68.1662 bits (165), Expect = 1.420e-13
Identity = 32/110 (29.09%), Postives = 62/110 (56.36%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            GN + V E LKG       + +G   +H+A +EG V + + LL    ++D   K+    +H AS  G +E++K+L+    N   ++++G+ P++ A+   H++++K LL+
Sbjct:   54 GNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLE 163          

HSP 4 Score: 65.0846 bits (157), Expect = 1.938e-12
Identity = 31/85 (36.47%), Postives = 50/85 (58.82%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R+     A LLL N+ N D ++K  + P+H A+  G+  +  LLL+R    +   ++G  P+H AS  G+  ++K+LLDR
Sbjct:  208 LHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDR 292          

HSP 5 Score: 58.5362 bits (140), Expect = 3.917e-10
Identity = 28/100 (28.00%), Postives = 54/100 (54.00%), Query Frame = 1
Query:  199 KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +G  VD      G+  +HIA   G  +V ++L+    NI+ +++  + P++ A++  H +++K LL+   N    T+ G+ P+  A   GH + + ILL+
Sbjct:   98 RGSSVDSATKK-GNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLE 196          

HSP 6 Score: 56.6102 bits (135), Expect = 1.808e-9
Identity = 27/83 (32.53%), Postives = 46/83 (55.42%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A   G   V +LLLD   N +  A   + P+H A +    ++++LL+    + +  T+SG  P+H A+  GHL I+ +LL
Sbjct:  373 LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL 455          

HSP 7 Score: 55.0694 bits (131), Expect = 6.199e-9
Identity = 27/91 (29.67%), Postives = 52/91 (57.14%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +DG  P+ +A ++G      +LL+N    D + K +   +H A+R   ++   LLL  + N + ++KSG+ P+H A+  G++ +  +LL+R
Sbjct:  173 EDGFTPLAVALQQGHNQAVAILLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNR 259          

HSP 8 Score: 46.9802 bits (110), Expect = 3.604e-6
Identity = 29/121 (23.97%), Postives = 47/121 (38.84%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRG---------------------------------GHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            DG  P+H A R G   V  LLL+    +    K    P+H A++G                                 GH  + KLLLD+  N      +G+ P+H A     ++++++L+
Sbjct:  302 DGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLV 422          
BLAST of SMED30027856 vs. Ensembl Mouse
Match: Ank2 (ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025])

HSP 1 Score: 78.5666 bits (192), Expect = 4.130e-17
Identity = 35/91 (38.46%), Postives = 58/91 (63.74%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            + G  P+HIA   G V+VA LLL+    +D  A+    P+H AS+ G++ ++KLLLDR    + +T+ G  P+H A+  GH +++++LL+R
Sbjct:  246 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLER 336          

HSP 2 Score: 75.8702 bits (185), Expect = 3.737e-16
Identity = 33/88 (37.50%), Postives = 54/88 (61.36%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A + G  ++ +LLLD    ID + +    P+H A+R GH ++++LLL+R       TK+G  P+H A+ G H+E +K LL
Sbjct:  280 NGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLL 367          

HSP 3 Score: 68.1662 bits (165), Expect = 1.821e-13
Identity = 32/110 (29.09%), Postives = 62/110 (56.36%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            GN + V E LKG       + +G   +H+A +EG V + + LL    ++D   K+    +H AS  G +E++K+L+    N   ++++G+ P++ A+   H++++K LL+
Sbjct:   57 GNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLE 166          

HSP 4 Score: 58.151 bits (139), Expect = 4.407e-10
Identity = 28/100 (28.00%), Postives = 54/100 (54.00%), Query Frame = 1
Query:  199 KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +G  VD      G+  +HIA   G  +V ++L+    NI+ +++  + P++ A++  H +++K LL+   N    T+ G+ P+  A   GH + + ILL+
Sbjct:  101 RGSSVDSATKK-GNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLE 199          

HSP 5 Score: 57.7658 bits (138), Expect = 7.110e-10
Identity = 31/93 (33.33%), Postives = 50/93 (53.76%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAK--------RKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R+     A LLL N+ N D ++K          + P+H A+  G+  +  LLL+R    +   ++G  P+H AS  G+  ++K+LLDR
Sbjct:  211 LHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDR 303          
BLAST of SMED30027856 vs. Ensembl Mouse
Match: Ank1 (ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88024])

HSP 1 Score: 77.7962 bits (190), Expect = 8.283e-17
Identity = 36/88 (40.91%), Postives = 54/88 (61.36%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIA R G V + RLLLD    I+   K +  P+H A+R GH  I ++LLD     + +TK+G  P+H A+ G HL+ +++LL
Sbjct:  242 NGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLL 329          

HSP 2 Score: 68.5514 bits (166), Expect = 1.442e-13
Identity = 35/93 (37.63%), Postives = 54/93 (58.06%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            V+   G  P+HIA     ++VA+LLL+   +++   +    P+H ASR G+  +++LLLDR    E  TK    P+H A+  GH+ I +ILLD
Sbjct:  205 VLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLD 297          

HSP 3 Score: 67.3958 bits (163), Expect = 3.523e-13
Identity = 33/89 (37.08%), Postives = 52/89 (58.43%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            GH P+H A REG VD A  LL+   +  C  K+ + P+H A++ G   + +LLL+ + +     K+G  P+H A    +L+I+K+LL R
Sbjct:  507 GHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPR 595          

HSP 4 Score: 66.2402 bits (160), Expect = 9.197e-13
Identity = 30/88 (34.09%), Postives = 53/88 (60.23%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G+ P+HIA ++  ++VAR LL    + + E+ +   P+H A++ GH+E++ LLL +  N     KSG  P+H  S  GH+ +  +L+
Sbjct:  605 NGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLI 692          

HSP 5 Score: 65.0846 bits (157), Expect = 1.981e-12
Identity = 29/90 (32.22%), Postives = 52/90 (57.78%), Query Frame = 1
Query:  226 DDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +  G  P+H+  +EG V VA +L+ +   +D   +  + P+H AS  G+ +++K LL    +   +TK G+ P+H A+  GH +I+ +LL
Sbjct:  669 NKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 758          

HSP 6 Score: 64.3142 bits (155), Expect = 4.350e-12
Identity = 31/86 (36.05%), Postives = 48/86 (55.81%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H A R G V ++ +LLD+   I  + K    PIH A++G H + ++LLL  N   +  T     P+H A+  GH  + K+LLD+
Sbjct:  279 PLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDK 364          

HSP 7 Score: 62.7734 bits (151), Expect = 1.322e-11
Identity = 36/96 (37.50%), Postives = 52/96 (54.17%), Query Frame = 1
Query:  223 IDD---DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            IDD   D   P+H+A   G   VA++LLD     +  A   + P+H A +  H  +++LLL    + +  T+SG  P+H AS  GHL I+K LL R
Sbjct:  335 IDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQR 430          

HSP 8 Score: 60.4622 bits (145), Expect = 8.474e-11
Identity = 32/88 (36.36%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LLL    +ID   +    P+H AS  GH  I+K LL R  +          P+H A+  GH E+ K LL
Sbjct:  374 NGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLL 461          

HSP 9 Score: 58.5362 bits (140), Expect = 3.524e-10
Identity = 29/88 (32.95%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  PIH+A +   +D  RLLL  N  ID        P+H A+  GH  + K+LLD+         +G+ P+H A    H+ ++++LL
Sbjct:  308 NGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLL 395          

HSP 10 Score: 57.7658 bits (138), Expect = 6.623e-10
Identity = 28/89 (31.46%), Postives = 48/89 (53.93%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            D   P+H A R G   + +LLL+N  + +        P+H A+R GH +    LL++  +  C TK G+ P+H A+  G + + ++LL+
Sbjct:  473 DDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLE 561          

HSP 11 Score: 56.6102 bits (135), Expect = 1.705e-9
Identity = 27/87 (31.03%), Postives = 47/87 (54.02%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G  P+H+A +EG  ++  LLL    N +   K    P+H  S+ GH  +  +L+      +  T+ G+ P+H AS  G+++++K LL
Sbjct:  639 GVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLL 725          

HSP 12 Score: 55.4546 bits (132), Expect = 4.919e-9
Identity = 31/86 (36.05%), Postives = 51/86 (59.30%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H+A R G  +VA+ LL N    + +AK    P+H A+R GH+ ++KLLL+   +    T +G  P+H A+  GH++    LL++
Sbjct:  444 PLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEK 529          

HSP 13 Score: 53.1434 bits (126), Expect = 2.924e-8
Identity = 27/111 (24.32%), Postives = 58/111 (52.25%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +GN +   ++L+        + +G   +H+A +EG V +   LL     ++   K+    +H A+  G  E+++ L++   N   +++ G+ P++ A+   HLE++K LL+
Sbjct:   26 SGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLE 136          

HSP 14 Score: 53.1434 bits (126), Expect = 3.247e-8
Identity = 28/85 (32.94%), Postives = 49/85 (57.65%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R      A +LL N+ N D  +K  + P+H A+   +  + +LLL+R  +     ++G  P+H AS  G++ ++++LLDR
Sbjct:  181 LHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDR 265          

HSP 15 Score: 52.373 bits (124), Expect = 5.277e-8
Identity = 29/100 (29.00%), Postives = 52/100 (52.00%), Query Frame = 1
Query:  196 LKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            L+ E     +   G  P+H+A + G V +A LLL+++ + +   K    P+H A    + +I+KLLL R  +      +G+ P+H A+    +E+ + LL
Sbjct:  527 LEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLL 626          

HSP 16 Score: 50.8322 bits (120), Expect = 1.621e-7
Identity = 24/91 (26.37%), Postives = 52/91 (57.14%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHL---EILKILLD 498
            G+ P+H+A   G + + + LL +  +++ + K  + P+H A++ GH++I+ LLL    +    + +G  P+  A   G++   ++LK++ D
Sbjct:  705 GYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTD 795          

HSP 17 Score: 50.0618 bits (118), Expect = 3.124e-7
Identity = 25/90 (27.78%), Postives = 46/90 (51.11%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A     +D+ +LLL    +    A   + P+H A++    E+ + LL    +   E+  G  P+H A+  GH E++ +LL +
Sbjct:  572 NGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSK 661          

HSP 18 Score: 48.521 bits (114), Expect = 9.759e-7
Identity = 29/99 (29.29%), Postives = 50/99 (50.51%), Query Frame = 1
Query:  202 GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  +D V +  G  P+H+A   G + + + LL    + +    +   P+H A+R GH+E+ K LL        + K    P+H A+  GH  ++K+LL+
Sbjct:  398 GASIDAVTES-GLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLE 495          
BLAST of SMED30027856 vs. UniProt/SwissProt
Match: sp|P16157|ANK1_HUMAN (Ankyrin-1 OS=Homo sapiens OX=9606 GN=ANK1 PE=1 SV=3)

HSP 1 Score: 78.1814 bits (191), Expect = 4.504e-16
Identity = 36/88 (40.91%), Postives = 55/88 (62.50%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIA R G V + RLLLD    I+ + K +  P+H A+R GH  I ++LLD     + +TK+G  P+H A+ G HL+ +++LL
Sbjct:  238 NGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLL 325          

HSP 2 Score: 74.7146 bits (182), Expect = 6.354e-15
Identity = 34/86 (39.53%), Postives = 54/86 (62.79%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H+A R G  +VA+ LL N   ++ +AK    P+H A+R GH+ ++KLLL+ N N    T +G  P+H A+  GH+E +  LL++
Sbjct:  440 PLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEK 525          

HSP 3 Score: 68.9366 bits (167), Expect = 7.768e-13
Identity = 32/90 (35.56%), Postives = 52/90 (57.78%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            D   P+H A R G  ++ +LLL+NN N +        P+H A+R GH E +  LL++  +  C TK G+ P+H A+  G + + ++LL+R
Sbjct:  469 DDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLER 558          

HSP 4 Score: 68.9366 bits (167), Expect = 7.843e-13
Identity = 35/93 (37.63%), Postives = 55/93 (59.14%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            V+   G  P+HIA     ++VA+LLL+   +++   +    P+H ASR G+  +++LLLDR    E +TK    P+H A+  GH+ I +ILLD
Sbjct:  201 VLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLD 293          

HSP 5 Score: 68.5514 bits (166), Expect = 9.146e-13
Identity = 33/89 (37.08%), Postives = 53/89 (59.55%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            GH P+HIA REG V+    LL+   +  C  K+ + P+H A++ G   + +LLL+R+ +     K+G  P+H A    +L+I+K+LL R
Sbjct:  503 GHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPR 591          

HSP 6 Score: 65.855 bits (159), Expect = 9.242e-12
Identity = 34/109 (31.19%), Postives = 60/109 (55.05%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+AE V   L  +    + +  G  P+H+  +EG V VA +L+ +   +D   +  + P+H AS  G+ +++K LL    +   +TK G+ P+H A+  GH +I+ +LL
Sbjct:  646 GHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 754          

HSP 7 Score: 65.4698 bits (158), Expect = 1.232e-11
Identity = 30/88 (34.09%), Postives = 53/88 (60.23%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G+ P+HIA ++  V+VAR LL    + + E+ +   P+H A++ GH+E++ LLL +  N     KSG  P+H  +  GH+ +  +L+
Sbjct:  601 NGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLI 688          

HSP 8 Score: 64.3142 bits (155), Expect = 3.184e-11
Identity = 37/99 (37.37%), Postives = 53/99 (53.54%), Query Frame = 1
Query:  214 DKVIDD---DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            D  IDD   D   P+H+A   G   VA++LLD     +  A   + P+H A +  H  +++LLL    + +  T+SG  P+H AS  GHL I+K LL R
Sbjct:  328 DAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQR 426          

HSP 9 Score: 62.3882 bits (150), Expect = 1.379e-10
Identity = 30/86 (34.88%), Postives = 48/86 (55.81%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H A R G V ++ +LLD+   I  + K    PIH A++G H + ++LLL  +   +  T     P+H A+  GH  + K+LLD+
Sbjct:  275 PLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDK 360          

HSP 10 Score: 60.8474 bits (146), Expect = 4.108e-10
Identity = 33/88 (37.50%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  V V  LLL    +ID   +    P+H AS  GH  I+K LL R  +          P+H A+  GH E+ K LL
Sbjct:  370 NGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLL 457          

HSP 11 Score: 56.6102 bits (135), Expect = 1.412e-8
Identity = 28/88 (31.82%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  PIH+A +   +D  RLLL  +  ID        P+H A+  GH  + K+LLD+         +G+ P+H A    H+ ++++LL
Sbjct:  304 NGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLL 391          

HSP 12 Score: 55.8398 bits (133), Expect = 2.779e-8
Identity = 33/109 (30.28%), Postives = 55/109 (50.46%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+ E V   L+ E     +   G  P+H+A + G V VA LLL+ + + +   K    P+H A    + +I+KLLL R  +      +G+ P+H A+    +E+ + LL
Sbjct:  514 GHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLL 622          

HSP 13 Score: 55.0694 bits (131), Expect = 4.652e-8
Identity = 29/99 (29.29%), Postives = 50/99 (50.51%), Query Frame = 1
Query:  202 GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  +D V +  G  P+H+A   G + + + LL    + +    +   P+H A+R GH+E+ K LL        + K    P+H A+  GH  ++K+LL+
Sbjct:  394 GASIDAVTES-GLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLE 491          

HSP 14 Score: 54.6842 bits (130), Expect = 6.620e-8
Identity = 26/87 (29.89%), Postives = 47/87 (54.02%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G  P+H+A +EG  ++  LLL    N +   K    P+H  ++ GH  +  +L+      +  T+ G+ P+H AS  G+++++K LL
Sbjct:  635 GVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLL 721          

HSP 15 Score: 53.1434 bits (126), Expect = 2.137e-7
Identity = 27/111 (24.32%), Postives = 58/111 (52.25%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +GN +   ++L+        + +G   +H+A +EG V +   LL     ++   K+    +H A+  G  E+++ L++   N   +++ G+ P++ A+   HLE++K LL+
Sbjct:   22 SGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLE 132          

HSP 16 Score: 53.1434 bits (126), Expect = 2.373e-7
Identity = 28/85 (32.94%), Postives = 49/85 (57.65%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R      A +LL N+ N D  +K  + P+H A+   +  + +LLL+R  +     ++G  P+H AS  G++ ++++LLDR
Sbjct:  177 LHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDR 261          

HSP 17 Score: 50.447 bits (119), Expect = 1.575e-6
Identity = 24/91 (26.37%), Postives = 51/91 (56.04%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHL---EILKILLD 498
            G+ P+H+A   G + + + LL +  +++ + K  + P+H A++ GH++I+ LLL    +    +  G  P+  A   G++   ++LK++ D
Sbjct:  701 GYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVLKVVTD 791          

HSP 18 Score: 50.447 bits (119), Expect = 1.959e-6
Identity = 25/90 (27.78%), Postives = 46/90 (51.11%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A     +D+ +LLL    +    A   + P+H A++    E+ + LL    +   E+  G  P+H A+  GH E++ +LL +
Sbjct:  568 NGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSK 657          
BLAST of SMED30027856 vs. UniProt/SwissProt
Match: sp|Q02357|ANK1_MOUSE (Ankyrin-1 OS=Mus musculus OX=10090 GN=Ank1 PE=1 SV=2)

HSP 1 Score: 77.7962 bits (190), Expect = 6.067e-16
Identity = 36/88 (40.91%), Postives = 54/88 (61.36%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIA R G V + RLLLD    I+   K +  P+H A+R GH  I ++LLD     + +TK+G  P+H A+ G HL+ +++LL
Sbjct:  234 NGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLL 321          

HSP 2 Score: 68.5514 bits (166), Expect = 1.056e-12
Identity = 35/93 (37.63%), Postives = 54/93 (58.06%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            V+   G  P+HIA     ++VA+LLL+   +++   +    P+H ASR G+  +++LLLDR    E  TK    P+H A+  GH+ I +ILLD
Sbjct:  197 VLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGHVRISEILLD 289          

HSP 3 Score: 67.3958 bits (163), Expect = 2.578e-12
Identity = 33/89 (37.08%), Postives = 52/89 (58.43%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            GH P+H A REG VD A  LL+   +  C  K+ + P+H A++ G   + +LLL+ + +     K+G  P+H A    +L+I+K+LL R
Sbjct:  499 GHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPR 587          

HSP 4 Score: 66.2402 bits (160), Expect = 6.730e-12
Identity = 30/88 (34.09%), Postives = 53/88 (60.23%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G+ P+HIA ++  ++VAR LL    + + E+ +   P+H A++ GH+E++ LLL +  N     KSG  P+H  S  GH+ +  +L+
Sbjct:  597 NGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLI 684          

HSP 5 Score: 65.0846 bits (157), Expect = 1.450e-11
Identity = 29/90 (32.22%), Postives = 52/90 (57.78%), Query Frame = 1
Query:  226 DDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +  G  P+H+  +EG V VA +L+ +   +D   +  + P+H AS  G+ +++K LL    +   +TK G+ P+H A+  GH +I+ +LL
Sbjct:  661 NKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLL 750          

HSP 6 Score: 64.3142 bits (155), Expect = 3.182e-11
Identity = 31/86 (36.05%), Postives = 48/86 (55.81%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H A R G V ++ +LLD+   I  + K    PIH A++G H + ++LLL  N   +  T     P+H A+  GH  + K+LLD+
Sbjct:  271 PLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDK 356          

HSP 7 Score: 62.7734 bits (151), Expect = 9.671e-11
Identity = 36/96 (37.50%), Postives = 52/96 (54.17%), Query Frame = 1
Query:  223 IDD---DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            IDD   D   P+H+A   G   VA++LLD     +  A   + P+H A +  H  +++LLL    + +  T+SG  P+H AS  GHL I+K LL R
Sbjct:  327 IDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQR 422          

HSP 8 Score: 60.4622 bits (145), Expect = 6.196e-10
Identity = 32/88 (36.36%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LLL    +ID   +    P+H AS  GH  I+K LL R  +          P+H A+  GH E+ K LL
Sbjct:  366 NGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLL 453          

HSP 9 Score: 58.5362 bits (140), Expect = 2.576e-9
Identity = 29/88 (32.95%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  PIH+A +   +D  RLLL  N  ID        P+H A+  GH  + K+LLD+         +G+ P+H A    H+ ++++LL
Sbjct:  300 NGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLL 387          

HSP 10 Score: 57.7658 bits (138), Expect = 4.841e-9
Identity = 28/89 (31.46%), Postives = 48/89 (53.93%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            D   P+H A R G   + +LLL+N  + +        P+H A+R GH +    LL++  +  C TK G+ P+H A+  G + + ++LL+
Sbjct:  465 DDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLE 553          

HSP 11 Score: 56.6102 bits (135), Expect = 1.246e-8
Identity = 27/87 (31.03%), Postives = 47/87 (54.02%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G  P+H+A +EG  ++  LLL    N +   K    P+H  S+ GH  +  +L+      +  T+ G+ P+H AS  G+++++K LL
Sbjct:  631 GVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTPLHVASHYGNIKLVKFLL 717          

HSP 12 Score: 55.4546 bits (132), Expect = 3.560e-8
Identity = 31/86 (36.05%), Postives = 51/86 (59.30%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H+A R G  +VA+ LL N    + +AK    P+H A+R GH+ ++KLLL+   +    T +G  P+H A+  GH++    LL++
Sbjct:  436 PLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGASPNLATTAGHTPLHTAAREGHVDTALALLEK 521          

HSP 13 Score: 53.1434 bits (126), Expect = 2.136e-7
Identity = 27/111 (24.32%), Postives = 58/111 (52.25%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +GN +   ++L+        + +G   +H+A +EG V +   LL     ++   K+    +H A+  G  E+++ L++   N   +++ G+ P++ A+   HLE++K LL+
Sbjct:   18 SGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLE 128          

HSP 14 Score: 53.1434 bits (126), Expect = 2.371e-7
Identity = 28/85 (32.94%), Postives = 49/85 (57.65%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R      A +LL N+ N D  +K  + P+H A+   +  + +LLL+R  +     ++G  P+H AS  G++ ++++LLDR
Sbjct:  173 LHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDR 257          

HSP 15 Score: 52.373 bits (124), Expect = 3.853e-7
Identity = 29/100 (29.00%), Postives = 52/100 (52.00%), Query Frame = 1
Query:  196 LKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            L+ E     +   G  P+H+A + G V +A LLL+++ + +   K    P+H A    + +I+KLLL R  +      +G+ P+H A+    +E+ + LL
Sbjct:  519 LEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLL 618          

HSP 16 Score: 50.8322 bits (120), Expect = 1.184e-6
Identity = 24/91 (26.37%), Postives = 52/91 (57.14%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHL---EILKILLD 498
            G+ P+H+A   G + + + LL +  +++ + K  + P+H A++ GH++I+ LLL    +    + +G  P+  A   G++   ++LK++ D
Sbjct:  697 GYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIAKRLGYISVTDVLKVVTD 787          

HSP 17 Score: 50.0618 bits (118), Expect = 2.280e-6
Identity = 25/90 (27.78%), Postives = 46/90 (51.11%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A     +D+ +LLL    +    A   + P+H A++    E+ + LL    +   E+  G  P+H A+  GH E++ +LL +
Sbjct:  564 NGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQGVTPLHLAAQEGHTEMVALLLSK 653          

HSP 18 Score: 48.9062 bits (115), Expect = 6.413e-6
Identity = 29/99 (29.29%), Postives = 50/99 (50.51%), Query Frame = 1
Query:  202 GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  +D V +  G  P+H+A   G + + + LL    + +    +   P+H A+R GH+E+ K LL        + K    P+H A+  GH  ++K+LL+
Sbjct:  390 GASIDAVTES-GLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLE 487          
BLAST of SMED30027856 vs. UniProt/SwissProt
Match: sp|O70511|ANK3_RAT (Ankyrin-3 OS=Rattus norvegicus OX=10116 GN=Ank3 PE=1 SV=3)

HSP 1 Score: 77.0258 bits (188), Expect = 1.282e-15
Identity = 34/91 (37.36%), Postives = 57/91 (62.64%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            + G  P+HIA   G ++VA LLL+    +D  A+    P+H AS+ G++ ++KLLLDR    + +T+ G  P+H  +  GH +++++LLDR
Sbjct:  241 ESGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDR 331          

HSP 2 Score: 73.1738 bits (178), Expect = 2.483e-14
Identity = 30/84 (35.71%), Postives = 52/84 (61.90%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            P+H+A + G  ++ +LLLD    ID + +    P+H  +R GH +++++LLDR      +TK+G  P+H A+ G HL  +++LL
Sbjct:  279 PLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLL 362          

HSP 3 Score: 67.3958 bits (163), Expect = 2.672e-12
Identity = 32/110 (29.09%), Postives = 61/110 (55.45%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G+ E   + +K      + + +G   +H+A +EG V+V   LL    N+D   K+    +H AS  G +E++K+L+    N   ++++G+ P++ A+   HLE+++ LLD
Sbjct:   52 GHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLD 161          

HSP 4 Score: 65.4698 bits (158), Expect = 1.392e-11
Identity = 36/113 (31.86%), Postives = 59/113 (52.21%), Query Frame = 1
Query:  166 NGNAEGVRENLK-GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G AE VR  ++ G  V+    DD   P+HI+ R G  D+ + LL    + +      + P+H ++R GH ++   LLD   +    TK G+ P+H A+  G LE+  +LL +
Sbjct:  484 SGQAEVVRYLVQDGAQVEAKAKDD-QTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQK 595          

HSP 5 Score: 64.6994 bits (156), Expect = 2.062e-11
Identity = 28/90 (31.11%), Postives = 52/90 (57.78%), Query Frame = 1
Query:  226 DDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +  G  P+H+A +E  V+VA +L++   ++D + K  + P+H     G+ +I+  LL  +     +TK+G+ P+H A+  GH  I+ +LL
Sbjct:  702 NKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLL 791          

HSP 6 Score: 64.6994 bits (156), Expect = 2.358e-11
Identity = 34/111 (30.63%), Postives = 62/111 (55.86%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G A+ V++ L+           G+ P+H++ REG  DVA  LLD+  ++    K+ + P+H A++ G  E+  LLL ++ + +   KSG  P+H A+   + ++  +LLD+
Sbjct:  518 GKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQ 628          

HSP 7 Score: 61.6178 bits (148), Expect = 2.662e-10
Identity = 33/114 (28.95%), Postives = 59/114 (51.75%), Query Frame = 1
Query:  157 QLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            QLM+  A     N++GE             +H+A R G  +V R L+ +   ++ +AK    P+H ++R G ++I++ LL +  +    T SG+ P+H ++  GH ++   LLD
Sbjct:  459 QLMHHGASPNTTNVRGETA-----------LHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLD 561          

HSP 8 Score: 59.6918 bits (143), Expect = 1.118e-9
Identity = 26/90 (28.89%), Postives = 53/90 (58.89%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+ P+HIA ++  +D+A  LL+   + +   ++    +H A++ GH +++ LLL RN N     KSG  P+H A+    + + ++L+++
Sbjct:  638 NGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQ 727          

HSP 9 Score: 58.5362 bits (140), Expect = 3.170e-9
Identity = 30/110 (27.27%), Postives = 60/110 (54.55%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G+ E V E L+ E         G+  +HIA   G  +V ++L+ N  N++ +++  + P++ A++  H E+++ LLD   +    T+ G+ P+  A   GH +++ +LL+
Sbjct:   85 GHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLE 194          

HSP 10 Score: 57.7658 bits (138), Expect = 5.161e-9
Identity = 25/83 (30.12%), Postives = 49/83 (59.04%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A +EG VD+  LLL  N N++   K    P+H A++     + ++L+++  + + +TK G+ P+H     G+++I+  LL
Sbjct:  676 VHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLL 758          

HSP 11 Score: 54.6842 bits (130), Expect = 7.119e-8
Identity = 26/83 (31.33%), Postives = 46/83 (55.42%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A   G   VA++LLD   N + +A   + P+H A +     +++LLL    + +  T+SG  P+H A+  GH+ I+  L+
Sbjct:  379 LHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLM 461          

HSP 12 Score: 54.299 bits (129), Expect = 7.610e-8
Identity = 27/88 (30.68%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A + G ++VA LLL  + + D   K    P+H A+   + ++  LLLD+  +     K+G+ P+H A+    ++I   LL+
Sbjct:  573 GFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLE 660          

HSP 13 Score: 51.9878 bits (123), Expect = 5.308e-7
Identity = 28/89 (31.46%), Postives = 45/89 (50.56%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  P+H+A       VA LLLD   +    AK  + P+H A++    +I   LL+   +    T+ G   VH A+  GH++++ +LL R
Sbjct:  606 GLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSR 694          

HSP 14 Score: 50.447 bits (119), Expect = 1.661e-6
Identity = 24/88 (27.27%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +   ++  +LLL +N  +D         +H A+  GH ++ K+LLD+  N   +  +G+ P+H A     + ++++LL
Sbjct:  341 NGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIRVMELLL 428          

HSP 15 Score: 49.6766 bits (117), Expect = 2.936e-6
Identity = 22/86 (25.58%), Postives = 46/86 (53.49%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+H+ C  G + +   LL ++  ++ + K  + P+H A++ GH+ I+ +LL  N +    T +G   +  A   G++ ++  L
Sbjct:  738 GYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNASPNELTVNGNTALAIARRLGYISVVDTL 823          

HSP 16 Score: 49.6766 bits (117), Expect = 3.385e-6
Identity = 30/99 (30.30%), Postives = 53/99 (53.54%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETK--------SGWRPVHWASSGGHLEILKILLDR 501
            +DG  P+ +A ++G   V  LLL+N    D + K +   +H A+R   ++   LLL  + N + E+K        SG+ P+H A+  G++ +  +LL+R
Sbjct:  171 EDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDTNADIESKMVVNRATESGFTPLHIAAHYGNINVATLLLNR 265          
BLAST of SMED30027856 vs. UniProt/SwissProt
Match: sp|Q8C8R3|ANK2_MOUSE (Ankyrin-2 OS=Mus musculus OX=10090 GN=Ank2 PE=1 SV=2)

HSP 1 Score: 76.6406 bits (187), Expect = 1.838e-15
Identity = 35/91 (38.46%), Postives = 58/91 (63.74%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            + G  P+HIA   G V+VA LLL+    +D  A+    P+H AS+ G++ ++KLLLDR    + +T+ G  P+H A+  GH +++++LL+R
Sbjct:  231 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLER 321          

HSP 2 Score: 74.3294 bits (181), Expect = 9.994e-15
Identity = 33/88 (37.50%), Postives = 54/88 (61.36%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A + G  ++ +LLLD    ID + +    P+H A+R GH ++++LLL+R       TK+G  P+H A+ G H+E +K LL
Sbjct:  265 NGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLL 352          

HSP 3 Score: 66.6254 bits (161), Expect = 5.403e-12
Identity = 31/88 (35.23%), Postives = 53/88 (60.23%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G   +H+A R G V+V R LL N   +D  A+ +  P+H ASR G +EI++LLL    + +  T +G+ P+H ++  G +++  +LL+
Sbjct:  464 GETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLE 551          

HSP 4 Score: 66.2402 bits (160), Expect = 7.486e-12
Identity = 32/110 (29.09%), Postives = 62/110 (56.36%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            GN + V E LKG       + +G   +H+A +EG V + + LL    ++D   K+    +H AS  G +E++K+L+    N   ++++G+ P++ A+   H++++K LL+
Sbjct:   42 GNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLE 151          

HSP 5 Score: 61.6178 bits (148), Expect = 2.802e-10
Identity = 36/112 (32.14%), Postives = 59/112 (52.68%), Query Frame = 1
Query:  169 GNAEGVRENLK-GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  E VR  L+ G  VD    ++   P+HIA R G  ++ +LLL +  + D      + P+H ++R G  ++  +LL+        TK G+ P+H A+  G L++ K+LL R
Sbjct:  475 GQVEVVRCLLRNGALVDARAREE-QTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQR 585          

HSP 6 Score: 60.077 bits (144), Expect = 8.028e-10
Identity = 30/90 (33.33%), Postives = 53/90 (58.89%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+ P+HI+ REG VDVA +LL+         K+ + P+H A++ G  ++ KLLL R    +   K+G  P+H A+   + ++  +LL++
Sbjct:  529 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEK 618          

HSP 7 Score: 59.3066 bits (142), Expect = 1.614e-9
Identity = 28/87 (32.18%), Postives = 49/87 (56.32%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            +G+ P+HIA ++  + +A  LL+     +   K+   P+H AS+ GH++++ LLLD+  N    TKSG   +H A+    + +  IL
Sbjct:  628 NGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADIL 714          

HSP 8 Score: 56.6102 bits (135), Expect = 1.553e-8
Identity = 27/88 (30.68%), Postives = 47/88 (53.41%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A + G +DVA+LLL      D   K    P+H A+   + ++  LLL++  +     K+G+ P+H A+    ++I   LL+
Sbjct:  563 GFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLN 650          

HSP 9 Score: 55.0694 bits (131), Expect = 4.740e-8
Identity = 29/90 (32.22%), Postives = 46/90 (51.11%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A       VA LLL+   +    AK  + P+H A++    +I   LL+        TK G  P+H AS  GH +++ +LLD+
Sbjct:  595 NGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDK 684          

HSP 10 Score: 54.6842 bits (130), Expect = 6.430e-8
Identity = 31/93 (33.33%), Postives = 50/93 (53.76%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAK--------RKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R+     A LLL N+ N D ++K          + P+H A+  G+  +  LLL+R    +   ++G  P+H AS  G+  ++K+LLDR
Sbjct:  196 LHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDR 288          

HSP 11 Score: 54.299 bits (129), Expect = 8.239e-8
Identity = 27/83 (32.53%), Postives = 46/83 (55.42%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A   G   V +LLLD   N +  A   + P+H A +    ++++LL+    + +  T+SG  P+H A+  GHL I+ +LL
Sbjct:  369 LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL 451          

HSP 12 Score: 53.9138 bits (128), Expect = 1.172e-7
Identity = 26/83 (31.33%), Postives = 47/83 (56.63%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A +E  V+VA +L  +  + D   K  + P+  A   G+ +++  LL +  N   +TK+G+ P+H A+  GH  I+ +LL
Sbjct:  699 LHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLL 781          

HSP 13 Score: 53.1434 bits (126), Expect = 2.240e-7
Identity = 28/100 (28.00%), Postives = 54/100 (54.00%), Query Frame = 1
Query:  199 KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +G  VD      G+  +HIA   G  +V ++L+    NI+ +++  + P++ A++  H +++K LL+   N    T+ G+ P+  A   GH + + ILL+
Sbjct:   86 RGSSVDSATKK-GNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLE 184          

HSP 14 Score: 51.2174 bits (121), Expect = 9.971e-7
Identity = 27/88 (30.68%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LL+    +I    +    PIH A+  GH  I+ LLL    + +     G   +H A+  G +E+++ LL
Sbjct:  397 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLL 484          

HSP 15 Score: 48.521 bits (114), Expect = 8.598e-6
Identity = 23/86 (26.74%), Postives = 43/86 (50.00%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+ +AC  G V +   LL    N++ + K  + P+H A++ GH+ I+ +LL         T +G   +  A   G++ ++  L
Sbjct:  728 GYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 813          
BLAST of SMED30027856 vs. UniProt/SwissProt
Match: sp|Q01484|ANK2_HUMAN (Ankyrin-2 OS=Homo sapiens OX=9606 GN=ANK2 PE=1 SV=4)

HSP 1 Score: 76.6406 bits (187), Expect = 1.839e-15
Identity = 35/91 (38.46%), Postives = 58/91 (63.74%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            + G  P+HIA   G V+VA LLL+    +D  A+    P+H AS+ G++ ++KLLLDR    + +T+ G  P+H A+  GH +++++LL+R
Sbjct:  231 ESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLER 321          

HSP 2 Score: 74.3294 bits (181), Expect = 1.090e-14
Identity = 33/88 (37.50%), Postives = 54/88 (61.36%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A + G  ++ +LLLD    ID + +    P+H A+R GH ++++LLL+R       TK+G  P+H A+ G H+E +K LL
Sbjct:  265 NGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLL 352          

HSP 3 Score: 66.6254 bits (161), Expect = 5.405e-12
Identity = 31/88 (35.23%), Postives = 53/88 (60.23%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G   +H+A R G V+V R LL N   +D  A+ +  P+H ASR G +EI++LLL    + +  T +G+ P+H ++  G +++  +LL+
Sbjct:  464 GETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLE 551          

HSP 4 Score: 66.2402 bits (160), Expect = 7.489e-12
Identity = 32/110 (29.09%), Postives = 62/110 (56.36%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            GN + V E LKG       + +G   +H+A +EG V + + LL    ++D   K+    +H AS  G +E++K+L+    N   ++++G+ P++ A+   H++++K LL+
Sbjct:   42 GNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLE 151          

HSP 5 Score: 61.6178 bits (148), Expect = 2.803e-10
Identity = 36/112 (32.14%), Postives = 59/112 (52.68%), Query Frame = 1
Query:  169 GNAEGVRENLK-GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  E VR  L+ G  VD    ++   P+HIA R G  ++ +LLL +  + D      + P+H ++R G  ++  +LL+        TK G+ P+H A+  G L++ K+LL R
Sbjct:  475 GQVEVVRCLLRNGALVDARAREE-QTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQR 585          

HSP 6 Score: 60.077 bits (144), Expect = 8.031e-10
Identity = 30/90 (33.33%), Postives = 53/90 (58.89%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+ P+HI+ REG VDVA +LL+         K+ + P+H A++ G  ++ KLLL R    +   K+G  P+H A+   + ++  +LL++
Sbjct:  529 NGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEK 618          

HSP 7 Score: 59.3066 bits (142), Expect = 1.828e-9
Identity = 28/87 (32.18%), Postives = 49/87 (56.32%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            +G+ P+HIA ++  + +A  LL+     +   K+   P+H AS+ GH++++ LLLD+  N    TKSG   +H A+    + +  IL
Sbjct:  628 NGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADIL 714          

HSP 8 Score: 56.6102 bits (135), Expect = 1.553e-8
Identity = 27/88 (30.68%), Postives = 47/88 (53.41%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A + G +DVA+LLL      D   K    P+H A+   + ++  LLL++  +     K+G+ P+H A+    ++I   LL+
Sbjct:  563 GFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLN 650          

HSP 9 Score: 55.0694 bits (131), Expect = 5.367e-8
Identity = 29/90 (32.22%), Postives = 46/90 (51.11%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A       VA LLL+   +    AK  + P+H A++    +I   LL+        TK G  P+H AS  GH +++ +LLD+
Sbjct:  595 NGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDK 684          

HSP 10 Score: 54.6842 bits (130), Expect = 5.792e-8
Identity = 26/83 (31.33%), Postives = 47/83 (56.63%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A +E  V+VA +L  +  + D   K  + P+  A   G+ +++  LL +  N   +TK+G+ P+H A+  GH  I+ +LL
Sbjct:  699 LHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLL 781          

HSP 11 Score: 54.6842 bits (130), Expect = 6.433e-8
Identity = 31/93 (33.33%), Postives = 50/93 (53.76%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAK--------RKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R+     A LLL N+ N D ++K          + P+H A+  G+  +  LLL+R    +   ++G  P+H AS  G+  ++K+LLDR
Sbjct:  196 LHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDR 288          

HSP 12 Score: 54.299 bits (129), Expect = 8.242e-8
Identity = 27/83 (32.53%), Postives = 46/83 (55.42%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A   G   V +LLLD   N +  A   + P+H A +    ++++LL+    + +  T+SG  P+H A+  GHL I+ +LL
Sbjct:  369 LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL 451          

HSP 13 Score: 53.1434 bits (126), Expect = 2.240e-7
Identity = 28/100 (28.00%), Postives = 54/100 (54.00%), Query Frame = 1
Query:  199 KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +G  VD      G+  +HIA   G  +V ++L+    NI+ +++  + P++ A++  H +++K LL+   N    T+ G+ P+  A   GH + + ILL+
Sbjct:   86 RGSSVDSATKK-GNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLE 184          

HSP 14 Score: 51.2174 bits (121), Expect = 9.975e-7
Identity = 27/88 (30.68%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LL+    +I    +    PIH A+  GH  I+ LLL    + +     G   +H A+  G +E+++ LL
Sbjct:  397 NGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLL 484          

HSP 15 Score: 48.521 bits (114), Expect = 8.602e-6
Identity = 23/86 (26.74%), Postives = 43/86 (50.00%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+ +AC  G V +   LL    N++ + K  + P+H A++ GH+ I+ +LL         T +G   +  A   G++ ++  L
Sbjct:  728 GYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIAKRLGYISVVDTL 813          
BLAST of SMED30027856 vs. TrEMBL
Match: Q16FJ0 (AAEL014741-PA (Fragment) OS=Aedes aegypti OX=7159 GN=AAEL014741 PE=4 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.619e-21
Identity = 40/91 (43.96%), Postives = 58/91 (63.74%), Query Frame = 1
Query:  226 DDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            DD+G  P+H A + G ++V +LL+DN  N+D    + W P+H AS+ GH E++KLL+D   N       GW P+H AS  GHLE++K L+D
Sbjct:   42 DDNGWTPLHRASQNGHLEVVKLLIDNRANVDTTQNKGWTPLHVASQNGHLEVVKLLIDNGANVYTTQNEGWTPLHVASLNGHLEVVKSLID 132          

HSP 2 Score: 93.2041 bits (230), Expect = 2.510e-20
Identity = 37/90 (41.11%), Postives = 57/90 (63.33%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            ++G  P+H+A   G ++V + L+DN  N+D    + W P+H AS+ GH E++KLL+D   N       GW P+H AS  GHLE++K+L+D
Sbjct:  109 NEGWTPLHVASLNGHLEVVKSLIDNRANVDTTQNKGWTPLHVASQNGHLEVVKLLIDNGANVYTTENEGWTPLHVASQNGHLEVVKLLID 198          

HSP 3 Score: 89.7373 bits (221), Expect = 4.785e-19
Identity = 40/111 (36.04%), Postives = 62/111 (55.86%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            NG+ E V+  +          + G  P+H+A + G ++V +LL+DN  N+       W P+H AS  GH E++K L+D   N +     GW P+H AS  GHLE++K+L+D
Sbjct:   55 NGHLEVVKLLIDNRANVDTTQNKGWTPLHVASQNGHLEVVKLLIDNGANVYTTQNEGWTPLHVASLNGHLEVVKSLIDNRANVDTTQNKGWTPLHVASQNGHLEVVKLLID 165          

HSP 4 Score: 88.1965 bits (217), Expect = 2.029e-18
Identity = 33/84 (39.29%), Postives = 55/84 (65.48%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +++    G++++ + L+D+N NID +    W P+H AS+ GH E++KLL+D   N +     GW P+H AS  GHLE++K+L+D
Sbjct:   16 VYLTADNGYIEMVKFLIDHNANIDTKDDNGWTPLHRASQNGHLEVVKLLIDNRANVDTTQNKGWTPLHVASQNGHLEVVKLLID 99          

HSP 5 Score: 67.0106 bits (162), Expect = 2.289e-10
Identity = 29/90 (32.22%), Postives = 50/90 (55.56%), Query Frame = 1
Query:  163 MNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKS 432
            +NG+ E V+  +          + G  P+H+A + G ++V +LL+DN  N+       W P+H AS+ GH E++KLL+D   N + +T +
Sbjct:  120 LNGHLEVVKSLIDNRANVDTTQNKGWTPLHVASQNGHLEVVKLLIDNGANVYTTENEGWTPLHVASQNGHLEVVKLLIDNRANVDAKTAN 209          

HSP 6 Score: 55.8398 bits (133), Expect = 2.382e-6
Identity = 21/60 (35.00%), Postives = 40/60 (66.67%), Query Frame = 1
Query:  319 CEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
             + K++ + ++  +  G+ E++K L+D N N + +  +GW P+H AS  GHLE++K+L+D
Sbjct:    7 SKLKQQMQLVYLTADNGYIEMVKFLIDHNANIDTKDDNGWTPLHRASQNGHLEVVKLLID 66          
BLAST of SMED30027856 vs. TrEMBL
Match: J9HSB7 (AAEL017480-PA (Fragment) OS=Aedes aegypti OX=7159 GN=AaeL_AAEL017480 PE=4 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 3.157e-21
Identity = 44/100 (44.00%), Postives = 62/100 (62.00%), Query Frame = 1
Query:  199 KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
             G  VD   D++G  P+H A R G ++V + L+DN  N+D      W P+HYASR GH E++KLL+D   N +     GW P+H AS  GHLE++K+L+D
Sbjct:  163 NGANVDTT-DNEGWTPLHYASRNGRLEVVKFLIDNGANVDTTQNEGWTPLHYASRNGHLEVVKLLIDDEANVDTTDNEGWTPLHDASLIGHLEVVKLLID 261          

HSP 2 Score: 97.0561 bits (240), Expect = 6.651e-21
Identity = 45/113 (39.82%), Postives = 69/113 (61.06%), Query Frame = 1
Query:  163 MNGNAEGVRENL-KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +NG+ E V+  +  G  VD    + G  P+H A + G ++V +LL+DN  N+D     +W P+HYASR G  E++K L+D   N +     GW P+H+AS  GHLE++K+L+D
Sbjct:   18 LNGHLEVVKLLIDNGANVDTT-QNKGWTPLHFASQNGHLEVVKLLIDNRANVDTTQNEEWTPLHYASRNGRLEVVKFLIDNGANVDTTDNEGWTPLHYASRNGHLEVVKLLID 129          

HSP 3 Score: 95.5153 bits (236), Expect = 2.446e-20
Identity = 41/100 (41.00%), Postives = 60/100 (60.00%), Query Frame = 1
Query:  199 KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
             G  VD   D++G  P+H A R G ++V +LL+DN  N+D      W P+HYASR G  E++K ++D   N +     GW P+H+AS  G LE++K L+D
Sbjct:   97 NGANVDTT-DNEGWTPLHYASRNGHLEVVKLLIDNGANVDTTRNEGWTPLHYASRNGRLEVVKFMIDNGANVDTTDNEGWTPLHYASRNGRLEVVKFLID 195          

HSP 4 Score: 95.1301 bits (235), Expect = 3.773e-20
Identity = 37/90 (41.11%), Postives = 57/90 (63.33%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            ++G  P+H A R G ++V + ++DN  N+D      W P+HYASR G  E++K L+D   N +     GW P+H+AS  GHLE++K+L+D
Sbjct:  139 NEGWTPLHYASRNGRLEVVKFMIDNGANVDTTDNEGWTPLHYASRNGRLEVVKFLIDNGANVDTTQNEGWTPLHYASRNGHLEVVKLLID 228          

HSP 5 Score: 94.3597 bits (233), Expect = 7.768e-20
Identity = 37/85 (43.53%), Postives = 54/85 (63.53%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            P+H A R G ++V + L+DN  N+D      W P+HYASR GH E++KLL+D   N +     GW P+H+AS  G LE++K ++D
Sbjct:   78 PLHYASRNGRLEVVKFLIDNGANVDTTDNEGWTPLHYASRNGHLEVVKLLIDNGANVDTTRNEGWTPLHYASRNGRLEVVKFMID 162          

HSP 6 Score: 90.5077 bits (223), Expect = 2.002e-18
Identity = 36/90 (40.00%), Postives = 57/90 (63.33%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            ++G  P+H A   G ++V +LL+DN  N+D    + W P+H+AS+ GH E++KLL+D   N +      W P+H+AS  G LE++K L+D
Sbjct:    7 NEGWTPLHYASLNGHLEVVKLLIDNGANVDTTQNKGWTPLHFASQNGHLEVVKLLIDNRANVDTTQNEEWTPLHYASRNGRLEVVKFLID 96          

HSP 7 Score: 78.5666 bits (192), Expect = 4.546e-14
Identity = 40/111 (36.04%), Postives = 65/111 (58.56%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            NG+ E V+  +  E      D++G  P+H A   G ++V +LL+DN  N+D +  R+   +H AS+ G  E++KLL+D   N + +   G   +H AS  GHLE++K+L+D
Sbjct:  217 NGHLEVVKLLIDDEANVDTTDNEGWTPLHDASLIGHLEVVKLLIDNGANVDTKNTRRPTSLHIASQNGRLEVVKLLIDNGANVDTKNTRGSTSLHIASRNGHLEVVKLLID 327          

HSP 8 Score: 76.6406 bits (187), Expect = 2.384e-13
Identity = 41/102 (40.20%), Postives = 61/102 (59.80%), Query Frame = 1
Query:  199 KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRP--VHWASSGGHLEILKILLD 498
             G  VD    ++G  P+H A R G ++V +LL+D+  N+D      W P+H AS  GH E++KLL+D   N   +TK+  RP  +H AS  G LE++K+L+D
Sbjct:  196 NGANVDTT-QNEGWTPLHYASRNGHLEVVKLLIDDEANVDTTDNEGWTPLHDASLIGHLEVVKLLIDNGAN--VDTKNTRRPTSLHIASQNGRLEVVKLLID 294          

HSP 9 Score: 74.7146 bits (182), Expect = 1.017e-12
Identity = 29/63 (46.03%), Postives = 41/63 (65.08%), Query Frame = 1
Query:  310 NIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            N+D      W P+HYAS  GH E++KLL+D   N +     GW P+H+AS  GHLE++K+L+D
Sbjct:    1 NVDTTRNEGWTPLHYASLNGHLEVVKLLIDNGANVDTTQNKGWTPLHFASQNGHLEVVKLLID 63          

HSP 10 Score: 56.225 bits (134), Expect = 3.513e-6
Identity = 24/57 (42.11%), Postives = 37/57 (64.91%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFE 417
            +HIA + G ++V +LL+DN  N+D +  R    +H ASR GH E++KLL+D   N +
Sbjct:  277 LHIASQNGRLEVVKLLIDNGANVDTKNTRGSTSLHIASRNGHLEVVKLLIDNGANVD 333          
BLAST of SMED30027856 vs. TrEMBL
Match: E3Q4M0 (Uncharacterized protein OS=Colletotrichum graminicola (strain M1.001 / M2 / FGSC 10212) OX=645133 GN=GLRG_01179 PE=4 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.266e-19
Identity = 45/112 (40.18%), Postives = 67/112 (59.82%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            NG+ E V+  L       V + DG   IHIA + G+++V +LLLD   ++       W P+ +AS  GH E++KLLLD+  +      +GW P+ WASS GHLE++K+LLD+
Sbjct: 1092 NGHIEVVKLLLNNGADASVANKDGQTSIHIASQNGYIEVVKLLLDKGADVTVPDHNGWTPLIWASSNGHLEVVKLLLDKGADVTVLDHNGWTPLVWASSNGHLEVVKLLLDK 1203          

HSP 2 Score: 86.6557 bits (213), Expect = 1.409e-16
Identity = 39/94 (41.49%), Postives = 60/94 (63.83%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            V + DG  PI++A + G ++V +LLL+N  +     K     IH AS+ G+ E++KLLLD+  +      +GW P+ WASS GHLE++K+LLD+
Sbjct: 1077 VANKDGQTPIYMASQNGHIEVVKLLLNNGADASVANKDGQTSIHIASQNGYIEVVKLLLDKGADVTVPDHNGWTPLIWASSNGHLEVVKLLLDK 1170          

HSP 3 Score: 62.7734 bits (151), Expect = 2.603e-8
Identity = 29/85 (34.12%), Postives = 48/85 (56.47%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +  A + G  D+  +L++N  N     K    PI+ AS+ GH E++KLLL+   +     K G   +H AS  G++E++K+LLD+
Sbjct: 1053 LQAASQGGHTDIVHILIENGANASVANKDGQTPIYMASQNGHIEVVKLLLNNGADASVANKDGQTSIHIASQNGYIEVVKLLLDK 1137          

HSP 4 Score: 58.9214 bits (141), Expect = 6.613e-7
Identity = 33/94 (35.11%), Postives = 47/94 (50.00%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            V D +G  P+  A   G ++V +LLLD   ++       W P+ +AS  GH E++KLLLD+  +          P+  AS  GH  I K LL R
Sbjct: 1143 VPDHNGWTPLIWASSNGHLEVVKLLLDKGADVTVLDHNGWTPLVWASSNGHLEVVKLLLDKGADATVVDYYNITPLSVASREGHTSIAKFLLSR 1236          

HSP 5 Score: 55.8398 bits (133), Expect = 6.485e-6
Identity = 24/85 (28.24%), Postives = 49/85 (57.65%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCE-AKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +  A + G  D+  +L++   +++ +   R    +  AS+GGH++I+ +L++   N     K G  P++ AS  GH+E++K+LL+
Sbjct: 1019 LQAASQGGHADIVHILINKGADVNAQDGSRYGNALQAASQGGHTDIVHILIENGANASVANKDGQTPIYMASQNGHIEVVKLLLN 1103          
BLAST of SMED30027856 vs. TrEMBL
Match: Q16FI9 (AAEL014742-PA (Fragment) OS=Aedes aegypti OX=7159 GN=AAEL014742 PE=4 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 1.484e-19
Identity = 38/90 (42.22%), Postives = 60/90 (66.67%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            D+G  P+H+A   G ++V +LL+DN  N+D +    W P+H AS+ GH E++KLL++   N + +   GW P+H+AS  GHLE++K L+D
Sbjct:   83 DEGWTPLHLAAENGHLEVVKLLIDNRANVDTKKNGGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLID 172          

HSP 2 Score: 94.3597 bits (233), Expect = 2.623e-19
Identity = 37/90 (41.11%), Postives = 59/90 (65.56%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            D+G  P+H+A   G+++V +LL+DN  N+D      W P+H A+  GH E++KLL+D   N + +   GW P+H AS  GHLE++K+L++
Sbjct:   50 DEGWTPLHLAAENGYLEVVKLLIDNGANVDTTQDEGWTPLHLAAENGHLEVVKLLIDNRANVDTKKNGGWTPLHVASQNGHLEVVKLLIE 139          

HSP 3 Score: 93.5893 bits (231), Expect = 5.409e-19
Identity = 39/111 (35.14%), Postives = 67/111 (60.36%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            NG+ E V+  +          D+G  P+H+A + G ++V +LL++N  N+D +    W P+H+AS+ GH E++K L+D   N +     GW P+H A+  GHLE++K+L++
Sbjct:  161 NGHLEVVKFLIDNRANVDTTQDEGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTPLHLAAENGHLEVVKLLIE 271          

HSP 4 Score: 92.4337 bits (228), Expect = 1.534e-18
Identity = 41/111 (36.94%), Postives = 65/111 (58.56%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            NG+ E V+  +          D+G  P+H+A   G ++V +LL++N  N+D +    W P+H AS+ GH E++K L+D   N +     GW P+H AS  GHLE++K+L+D
Sbjct:  227 NGHLEVVKFLIDNRANVDTTQDEGWTPLHLAAENGHLEVVKLLIENRANVDTKKNGGWTPLHVASQNGHLEVVKFLIDNRANVDTTQYEGWTPLHVASQNGHLEVVKLLID 337          

HSP 5 Score: 92.0485 bits (227), Expect = 1.991e-18
Identity = 40/111 (36.04%), Postives = 66/111 (59.46%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            NG+ E V+  ++         ++G  P+H A + G ++V + L+DN  N+D      W P+H AS+ GH E++KLL++   N + +   GW P+H+AS  GHLE++K L+D
Sbjct:  128 NGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLID 238          

HSP 6 Score: 92.0485 bits (227), Expect = 2.069e-18
Identity = 40/111 (36.04%), Postives = 69/111 (62.16%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            NG+ E V+  +  +      D++G  P+H+A + G ++V +LL++N  N+D    +   P+H+AS+ GH E++KLL+D   N +     GW P+H AS  GHLE++K+L++
Sbjct:  568 NGHLEVVKLLIDNKANVDTTDNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIE 678          

HSP 7 Score: 91.6633 bits (226), Expect = 2.764e-18
Identity = 36/91 (39.56%), Postives = 58/91 (63.74%), Query Frame = 1
Query:  226 DDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            ++ G  P+H+A + G + V +LL+DN  N+D E    W P+H A+  G+ E++KLL+D   N +     GW P+H A+  GHLE++K+L+D
Sbjct:   16 NNGGRTPLHVASQNGHLKVVKLLIDNGANVDTEGDEGWTPLHLAAENGYLEVVKLLIDNGANVDTTQDEGWTPLHLAAENGHLEVVKLLID 106          

HSP 8 Score: 90.8929 bits (224), Expect = 5.027e-18
Identity = 40/111 (36.04%), Postives = 67/111 (60.36%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            NG+ E V+  +          ++G  P+H+A + G ++V +LL++N  N+D    +   P+H+AS+ GH E++KLL+D   N +     GW P+H AS  GHLE++K+L+D
Sbjct:  634 NGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLID 744          

HSP 9 Score: 90.5077 bits (223), Expect = 5.866e-18
Identity = 35/88 (39.77%), Postives = 58/88 (65.91%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A + G ++V +LL++N  N+D +    W P+H+AS+ GH E++K L+D   N +     GW P+H AS  GHLE++K+L++
Sbjct:  118 GWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTPLHVASQNGHLEVVKLLIE 205          

HSP 10 Score: 88.9669 bits (219), Expect = 2.399e-17
Identity = 39/111 (35.14%), Postives = 64/111 (57.66%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            NG+ E V+  ++         ++G  P+H A + G ++V + L+DN  N+D      W P+H A+  GH E++KLL++   N + +   GW P+H AS  GHLE++K L+D
Sbjct:  194 NGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTPLHLAAENGHLEVVKLLIENRANVDTKKNGGWTPLHVASQNGHLEVVKFLID 304          

HSP 11 Score: 88.1965 bits (217), Expect = 3.924e-17
Identity = 39/111 (35.14%), Postives = 66/111 (59.46%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            NG+ E V+  ++         ++G  P+++A   G ++V +LL++N  N+D      W P++ AS+ GH E++KLL+D   N +     GW P+H AS  GHLE++K+L+D
Sbjct:  436 NGHLEVVKLLIENRANVDTTQNEGWTPLYVASINGHLEVVKLLINNRANVDTTQNEGWTPLYVASKNGHLEVVKLLIDNKANVDTTQNEGWTPLHVASQNGHLEVVKLLID 546          

HSP 12 Score: 87.8113 bits (216), Expect = 4.946e-17
Identity = 40/111 (36.04%), Postives = 65/111 (58.56%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            NG+ E V+  ++         + G  P+H+A + G ++V + L+DN  N+D      W P+H AS+ GH E++KLL+D   N +     G  P+H+AS  GHLE++K+L+D
Sbjct:  260 NGHLEVVKLLIENRANVDTKKNGGWTPLHVASQNGHLEVVKFLIDNRANVDTTQYEGWTPLHVASQNGHLEVVKLLIDNKANVDTTQNKGITPLHFASQNGHLEVVKLLID 370          

HSP 13 Score: 87.8113 bits (216), Expect = 5.043e-17
Identity = 40/111 (36.04%), Postives = 65/111 (58.56%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            NG+ E V+  ++         + G  P+H A + G ++V +LL+DN  N+D      W P+H AS+ GH E++KLL++   N +     G  P+H+AS  GHLE++K+L+D
Sbjct:  601 NGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLID 711          

HSP 14 Score: 87.4261 bits (215), Expect = 6.933e-17
Identity = 39/111 (35.14%), Postives = 67/111 (60.36%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            NG+ E V+  +  +       ++G  P+H+A + G ++V +LL+DN  N+D    +   P++ AS+ GH E++KLL+D   N +     GW P+H AS  GHLE++K+L++
Sbjct:  502 NGHLEVVKLLIDNKANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTKNKGITPLYVASKNGHLEVVKLLIDNKANVDTTDNEGWTPLHVASQNGHLEVVKLLIE 612          

HSP 15 Score: 86.2705 bits (212), Expect = 1.784e-16
Identity = 39/112 (34.82%), Postives = 66/112 (58.93%), Query Frame = 1
Query:  163 MNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +NG+ E V+  +          ++G  P+++A + G ++V +LL+DN  N+D      W P+H AS+ GH E++KLL+D   N +     G  P++ AS  GHLE++K+L+D
Sbjct:  468 INGHLEVVKLLINNRANVDTTQNEGWTPLYVASKNGHLEVVKLLIDNKANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTKNKGITPLYVASKNGHLEVVKLLID 579          

HSP 16 Score: 86.2705 bits (212), Expect = 1.908e-16
Identity = 39/111 (35.14%), Postives = 65/111 (58.56%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            NG+ E V+  ++         ++G  P+H A R G ++V +LL++N  N+D      W P++ AS  GH E++KLL++   N +     GW P++ AS  GHLE++K+L+D
Sbjct:  403 NGHLEVVKLLIENRANVGTTQNEGWTPLHFASRNGHLEVVKLLIENRANVDTTQNEGWTPLYVASINGHLEVVKLLINNRANVDTTQNEGWTPLYVASKNGHLEVVKLLID 513          

HSP 17 Score: 86.2705 bits (212), Expect = 2.227e-16
Identity = 39/111 (35.14%), Postives = 65/111 (58.56%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            NG+ E V+  +          + G  P+++A + G ++V +LL+DN  N+D      W P+H AS+ GH E++KLL++   N +     G  P+H+AS  GHLE++K+L+D
Sbjct:  535 NGHLEVVKLLIDNRANVDTTKNKGITPLYVASKNGHLEVVKLLIDNKANVDTTDNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLID 645          

HSP 18 Score: 84.7297 bits (208), Expect = 6.012e-16
Identity = 40/111 (36.04%), Postives = 64/111 (57.66%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            NG+ E V+  ++         + G  P+H A + G ++V +LL+DN  N+D      W P+H AS+ GH E++KLL+D   N +     G  P++ AS  GHLE++K+L+D
Sbjct:  667 NGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTQNKGITPLYVASINGHLEVVKLLID 777          

HSP 19 Score: 83.9593 bits (206), Expect = 1.082e-15
Identity = 34/90 (37.78%), Postives = 57/90 (63.33%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            + G  P+H A + G ++V +LL++N  N+       W P+H+ASR GH E++KLL++   N +     GW P++ AS  GHLE++K+L++
Sbjct:  391 NKGITPLHFASQNGHLEVVKLLIENRANVGTTQNEGWTPLHFASRNGHLEVVKLLIENRANVDTTQNEGWTPLYVASINGHLEVVKLLIN 480          

HSP 20 Score: 78.1814 bits (191), Expect = 1.083e-13
Identity = 39/122 (31.97%), Postives = 66/122 (54.10%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNI-----------DCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            NG+ E V+  +  +       + G  P+H A + G ++V +LL+DN  N+           D    +   P+H+AS+ GH E++KLL++   N       GW P+H+AS  GHLE++K+L++
Sbjct:  326 NGHLEVVKLLIDNKANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIENRANVGTTQNEGWTPLHFASRNGHLEVVKLLIE 447          

HSP 21 Score: 77.411 bits (189), Expect = 2.600e-13
Identity = 35/100 (35.00%), Postives = 59/100 (59.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLD-----------RNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +G  P+H+A + G ++V +LL+DN  N+D    +   P+H+AS+ GH E++KLL+D              N +     G  P+H+AS  GHLE++K+L++
Sbjct:  315 EGWTPLHVASQNGHLEVVKLLIDNKANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIE 414          

HSP 22 Score: 69.707 bits (169), Expect = 1.124e-10
Identity = 29/73 (39.73%), Postives = 47/73 (64.38%), Query Frame = 1
Query:  280 VARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            + + L+D+N NID        P+H AS+ GH +++KLL+D   N + E   GW P+H A+  G+LE++K+L+D
Sbjct:    1 MVKFLIDHNANIDTANNGGRTPLHVASQNGHLKVVKLLIDNGANVDTEGDEGWTPLHLAAENGYLEVVKLLID 73          

HSP 23 Score: 57.3806 bits (137), Expect = 1.960e-6
Identity = 28/90 (31.11%), Postives = 48/90 (53.33%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSG 435
            NG+ E V+  +          ++G  P+H+A + G ++V +LL+DN  N+D    +   P++ AS  GH E++KLL+D   N +     G
Sbjct:  700 NGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTQNKGITPLYVASINGHLEVVKLLIDNRANVDTTQNEG 789          
BLAST of SMED30027856 vs. TrEMBL
Match: A0A090MA79 (WGS project CBMG000000000 data, contig CS5907-c003887 OS=Fusarium acuminatum CS5907 OX=1318461 GN=BN851_0152750 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.708e-19
Identity = 46/119 (38.66%), Postives = 68/119 (57.14%), Query Frame = 1
Query:  139 FECFKEQLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +    E  MN  +E VR  L+ E   + +DD G  P+H+A   G +   ++LLD+   +    K  W P+HYASR G++EI K+LL++  +       GW P+H AS  GHL+I+KILL
Sbjct:  107 WTPLHEVSMNDYSEVVRMLLQTEADTEAVDDAGWTPLHLASGNGHLATTKILLDSRVEVLAIDKYGWIPLHYASRKGYTEIAKVLLEKGPDTVVSNNDGWTPLHDASRKGHLDIIKILL 225          

HSP 2 Score: 73.1738 bits (178), Expect = 2.321e-12
Identity = 32/93 (34.41%), Postives = 51/93 (54.84%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            VID     P+H+  + G+ ++AR+++    +     K  W P+H  S   +SE++++LL    + E    +GW P+H AS  GHL   KILLD
Sbjct:   68 VIDKARLTPLHLTLKNGYSEMARIVVQKGADARAIMKYGWTPLHEVSMNDYSEVVRMLLQTEADTEAVDDAGWTPLHLASGNGHLATTKILLD 160          

HSP 3 Score: 70.4774 bits (171), Expect = 1.827e-11
Identity = 35/93 (37.63%), Postives = 53/93 (56.99%), Query Frame = 1
Query:  223 IDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            ID  G  P+H A R+G+ ++A++LL+   +        W P+H ASR GH +I+K+LLD   +       G  P+H AS  GH E +KIL ++
Sbjct:  168 IDKYGWIPLHYASRKGYTEIAKVLLEKGPDTVVSNNDGWTPLHDASRKGHLDIIKILLDNEADAAAADNDGLTPLHLASKNGHSEAVKILREK 260          

HSP 4 Score: 69.3218 bits (168), Expect = 5.539e-11
Identity = 38/126 (30.16%), Postives = 57/126 (45.24%), Query Frame = 1
Query:  124 IEMGEFECFKEQLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            I+          L NG +E  R  ++     + I   G  P+H      + +V R+LL    + +      W P+H AS  GH    K+LLD         K GW P+H+AS  G+ EI K+LL++
Sbjct:   69 IDKARLTPLHLTLKNGYSEMARIVVQKGADARAIMKYGWTPLHEVSMNDYSEVVRMLLQTEADTEAVDDAGWTPLHLASGNGHLATTKILLDSRVEVLAIDKYGWIPLHYASRKGYTEIAKVLLEK 194          

HSP 5 Score: 55.4546 bits (132), Expect = 4.877e-6
Identity = 24/93 (25.81%), Postives = 50/93 (53.76%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +V+   G   +H+A   G +  A++LL+   +I    K +  P+H   + G+SE+ ++++ +  +     K GW P+H  S   + E++++LL
Sbjct:   34 RVVTKYGWTSLHLASEYGRLKTAKILLEKGADITVIDKARLTPLHLTLKNGYSEMARIVVQKGADARAIMKYGWTPLHEVSMNDYSEVVRMLL 126          
BLAST of SMED30027856 vs. Ensembl Cavefish
Match: ank1a (ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GENE-041010-44])

HSP 1 Score: 79.7221 bits (195), Expect = 1.414e-17
Identity = 36/88 (40.91%), Postives = 56/88 (63.64%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIA R G V + RLLLD    ID + K +  P+H A+R GH  I+++LLD+    + +TK+G  P+H A+ G H++ ++ LL
Sbjct:  210 NGITPLHIASRRGNVIMVRLLLDRGATIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLL 297          

HSP 2 Score: 73.9442 bits (180), Expect = 1.381e-15
Identity = 35/85 (41.18%), Postives = 51/85 (60.00%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            P+H+A R G  +VA  LL N   +D +AK     +H A+R GH E++KLLL+   N    T +G  P+H A+  GH + ++ILLD
Sbjct:  412 PLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTSLHCAARMGHKELVKLLLEHKANPNSTTTAGHTPLHIAAREGHTQTVRILLD 496          

HSP 3 Score: 72.0182 bits (175), Expect = 7.948e-15
Identity = 36/93 (38.71%), Postives = 56/93 (60.22%), Query Frame = 1
Query:  223 IDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            I   G  P+HIA     ++VA+LLL+   N++   K    P+H ASR G+  +++LLLDR    + +TK    P+H A+  GH+ I++ILLD+
Sbjct:  174 ITATGFTPLHIAAHYENLNVAQLLLNRGANVNFTPKNGITPLHIASRRGNVIMVRLLLDRGATIDAKTKDELTPLHCAARNGHVRIIEILLDQ 266          

HSP 4 Score: 66.6254 bits (161), Expect = 4.848e-13
Identity = 31/89 (34.83%), Postives = 51/89 (57.30%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            GH P+HIA REG     R+LLD N       K+ + P+H A++ G  ++ +LLL+R  N     K+G  P+H A    +L+++ +L+ +
Sbjct:  475 GHTPLHIAAREGHTQTVRILLDMNAQQTKMTKKGFTPLHVAAKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSK 563          

HSP 5 Score: 65.4698 bits (158), Expect = 1.447e-12
Identity = 42/123 (34.15%), Postives = 62/123 (50.41%), Query Frame = 1
Query:  142 ECFKEQLMNGNAEGVRENLKGEFVDKVIDD---DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +C + QL+  NAE              IDD   D   P+H+A   G   +A++LLD     +  A   + P+H A +  H  ++ LLL  + + E  T+SG  P+H AS  GHL I+KILL +
Sbjct:  291 DCVR-QLLQYNAE--------------IDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNTRALNGFTPLHIACKKNHLRVMDLLLKHSASLEAVTESGLTPLHVASFMGHLNIVKILLQK 398          

HSP 6 Score: 62.3882 bits (150), Expect = 1.325e-11
Identity = 32/97 (32.99%), Postives = 55/97 (56.70%), Query Frame = 1
Query:  211 VDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            VD    DD    +H A R G  ++ +LLL++  N +        P+H A+R GH++ +++LLD N      TK G+ P+H A+  G +++ ++LL+R
Sbjct:  435 VDAKAKDD-QTSLHCAARMGHKELVKLLLEHKANPNSTTTAGHTPLHIAAREGHTQTVRILLDMNAQQTKMTKKGFTPLHVAAKYGKVDVAELLLER 530          

HSP 7 Score: 61.2326 bits (147), Expect = 4.180e-11
Identity = 26/90 (28.89%), Postives = 52/90 (57.78%), Query Frame = 1
Query:  226 DDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +  G  P+H+  +EG V +A +L+    ++    +  + P+H A   G+ +++K LL +  N   +T++G+ P+H A+  GH +I+ +LL
Sbjct:  637 NKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNGKTRAGYTPLHQAAQQGHTDIVTLLL 726          

HSP 8 Score: 60.8474 bits (146), Expect = 5.957e-11
Identity = 38/112 (33.93%), Postives = 56/112 (50.00%), Query Frame = 1
Query:  169 GNAEGVRENL-KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            GN   VR  L +G  +D    D+   P+H A R G V +  +LLD    I  + K    PIH A++G H + ++ LL  N   +  T     P+H A+  GH  + K+LLD+
Sbjct:  222 GNVIMVRLLLDRGATIDAKTKDE-LTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDK 332          

HSP 9 Score: 59.6918 bits (143), Expect = 1.464e-10
Identity = 28/90 (31.11%), Postives = 52/90 (57.78%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+ P+HIA ++  V+VA  LL    + + E+ +   P+H A++ G ++++ LL+ +  N     KSG  P+H  +  GH+ I  IL+ +
Sbjct:  573 NGYTPLHIASKQNQVEVASSLLQYGASANAESLQGVTPLHLAAQEGRADMVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQ 662          

HSP 10 Score: 58.151 bits (139), Expect = 4.151e-10
Identity = 29/88 (32.95%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LLL ++ +++   +    P+H AS  GH  I+K+LL +  +          P+H AS  GH E+ + LL
Sbjct:  342 NGFTPLHIACKKNHLRVMDLLLKHSASLEAVTESGLTPLHVASFMGHLNIVKILLQKGASPNASNVKVETPLHMASRAGHCEVAEFLL 429          

HSP 11 Score: 56.225 bits (134), Expect = 1.877e-9
Identity = 26/89 (29.21%), Postives = 49/89 (55.06%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  P+H+A +EG  D+  LL+    N++   K    P+H  ++ GH  I  +L+ +  +    T+ G+ P+H A   G+++++K LL +
Sbjct:  607 GVTPLHLAAQEGRADMVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQ 695          

HSP 12 Score: 56.225 bits (134), Expect = 2.251e-9
Identity = 26/94 (27.66%), Postives = 50/94 (53.19%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            + + + G  P+H+A   G +++ ++LL    + +    +   P+H ASR GH E+ + LL      + + K     +H A+  GH E++K+LL+
Sbjct:  370 EAVTESGLTPLHVASFMGHLNIVKILLQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTSLHCAARMGHKELVKLLLE 463          

HSP 13 Score: 55.8398 bits (133), Expect = 2.622e-9
Identity = 29/88 (32.95%), Postives = 44/88 (50.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  PIH+A +   +D  R LL  N  ID        P+H A+  GH  + K+LLD+         +G+ P+H A    HL ++ +LL
Sbjct:  276 NGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNTRALNGFTPLHIACKKNHLRVMDLLL 363          

HSP 14 Score: 54.6842 bits (130), Expect = 6.128e-9
Identity = 31/109 (28.44%), Postives = 53/109 (48.62%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+ + VR  L        +   G  P+H+A + G VDVA LLL+   N +   K    P+H A    + +++ LL+ +  +     ++G+ P+H AS    +E+   LL
Sbjct:  486 GHTQTVRILLDMNAQQTKMTKKGFTPLHVAAKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTPLHIASKQNQVEVASSLL 594          

HSP 15 Score: 53.9138 bits (128), Expect = 1.326e-8
Identity = 27/111 (24.32%), Postives = 58/111 (52.25%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +GN +   +++K        + +G   +H+A +EG V +   LL N   ++   K+    +H A+  G  +++  L++   N   +++ G+ P++ A+   HLE++K LL+
Sbjct:   20 SGNLDKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHNGVVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLE 130          

HSP 16 Score: 53.1434 bits (126), Expect = 2.559e-8
Identity = 23/86 (26.74%), Postives = 47/86 (54.65%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+H+AC  G + + + LL    N++ + +  + P+H A++ GH++I+ LLL         T +G   +  A   G++ ++ +L
Sbjct:  673 GYTPLHVACHYGNIKMVKFLLQQQANVNGKTRAGYTPLHQAAQQGHTDIVTLLLKHGAQPNEITTNGTSALAIAKRLGYISVIDVL 758          

HSP 17 Score: 46.9802 bits (110), Expect = 3.124e-6
Identity = 23/90 (25.56%), Postives = 44/90 (48.89%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A     +DV  LL+    +    A+  + P+H AS+    E+   LL    +   E+  G  P+H A+  G  +++ +L+ +
Sbjct:  540 NGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTPLHIASKQNQVEVASSLLQYGASANAESLQGVTPLHLAAQEGRADMVSLLISK 629          
BLAST of SMED30027856 vs. Ensembl Cavefish
Match: ank1b (ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GENE-091113-6])

HSP 1 Score: 78.9518 bits (193), Expect = 2.591e-17
Identity = 35/88 (39.77%), Postives = 55/88 (62.50%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIA R G V + RLLLD    ID   K +  P+H A+R GH  ++++LLD+    + +TK+G  P+H A+ G H++ ++ LL
Sbjct:  213 NGITPLHIAARRGNVIMVRLLLDRGAQIDARTKDELTPLHCAARNGHVRVIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLL 300          

HSP 2 Score: 77.0258 bits (188), Expect = 1.141e-16
Identity = 36/85 (42.35%), Postives = 52/85 (61.18%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            P+H+A R G  +VA+ LL N   +D +AK    P+H A+R GH E++KLLL+   N +  T +G  P+H A+  GH    +ILLD
Sbjct:  415 PLHMAARAGHCEVAQFLLQNLAQVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPDSATTAGHTPLHIAAREGHAHTTRILLD 499          

HSP 3 Score: 70.4774 bits (171), Expect = 2.540e-14
Identity = 34/94 (36.17%), Postives = 55/94 (58.51%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            V+   G  P+HIA     + VA+LLL+   N++   K    P+H A+R G+  +++LLLDR    +  TK    P+H A+  GH+ +++ILLD+
Sbjct:  176 VLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIAARRGNVIMVRLLLDRGAQIDARTKDELTPLHCAARNGHVRVIEILLDQ 269          

HSP 4 Score: 68.1662 bits (165), Expect = 1.459e-13
Identity = 32/89 (35.96%), Postives = 51/89 (57.30%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            GH P+HIA REG     R+LLD N       K+ + P+H A + G  ++ +LLL+R  N     K+G  P+H A    +L+++K+L+ +
Sbjct:  478 GHTPLHIAAREGHAHTTRILLDGNAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSK 566          

HSP 5 Score: 64.6994 bits (156), Expect = 2.082e-12
Identity = 34/97 (35.05%), Postives = 53/97 (54.64%), Query Frame = 1
Query:  211 VDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            VD    DD   P+H A R G  ++ +LLL++  N D        P+H A+R GH+   ++LLD N      TK G+ P+H A   G +++ ++LL+R
Sbjct:  438 VDAKAKDD-QTPLHCAARMGHKELVKLLLEHKANPDSATTAGHTPLHIAAREGHAHTTRILLDGNAQQTKMTKKGFTPLHVACKYGKVDVAELLLER 533          

HSP 6 Score: 63.5438 bits (153), Expect = 6.967e-12
Identity = 34/109 (31.19%), Postives = 55/109 (50.46%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+A   R  L G      +   G  P+H+AC+ G VDVA LLL+   N +   K    P+H A    + +++KLL+ +  +     ++G+ P+H A+    LE+   LL
Sbjct:  489 GHAHTTRILLDGNAQQTKMTKKGFTPLHVACKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTPLHIAAKQNQLEVASSLL 597          

HSP 7 Score: 62.003 bits (149), Expect = 1.998e-11
Identity = 41/123 (33.33%), Postives = 60/123 (48.78%), Query Frame = 1
Query:  142 ECFKEQLMNGNAEGVRENLKGEFVDKVIDD---DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +C + QL+  NAE              IDD   D   P+H+A   G   +A++LLD     +  A   + P+H A +  H   + LLL  + + E  T+SG  P+H A+  GHL I+K LL R
Sbjct:  294 DCVR-QLLQYNAE--------------IDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQR 401          

HSP 8 Score: 60.8474 bits (146), Expect = 5.513e-11
Identity = 31/86 (36.05%), Postives = 45/86 (52.33%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H A R G V V  +LLD    I  + K    PIH A++G H + ++ LL  N   +  T     P+H A+  GH  + K+LLD+
Sbjct:  250 PLHCAARNGHVRVIEILLDQGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDK 335          

HSP 9 Score: 59.3066 bits (142), Expect = 1.987e-10
Identity = 24/84 (28.57%), Postives = 50/84 (59.52%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            P+H+  +EG V +A +L+    ++   ++  +  +H A   G+ +++K LL +  +  C+T+ G+ P+H A+  GH +I+ +LL
Sbjct:  651 PLHLVAQEGHVGIADMLVKQGASVYSASRMGYTALHVACHYGNIKMVKFLLQQQAHVNCKTRVGYTPLHQAAQQGHTDIVTLLL 734          

HSP 10 Score: 56.9954 bits (136), Expect = 1.048e-9
Identity = 26/94 (27.66%), Postives = 50/94 (53.19%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            + + + G  P+H+A   G +++ + LL    + +    +   P+H A+R GH E+ + LL      + + K    P+H A+  GH E++K+LL+
Sbjct:  373 EAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLLQNLAQVDAKAKDDQTPLHCAARMGHKELVKLLLE 466          

HSP 11 Score: 55.4546 bits (132), Expect = 4.025e-9
Identity = 29/85 (34.12%), Postives = 49/85 (57.65%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R      A +LL N+ N D  +K  + P+H A+   +  + +LLL+R  N     K+G  P+H A+  G++ ++++LLDR
Sbjct:  152 LHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLSVAQLLLNRGANVNFTPKNGITPLHIAARRGNVIMVRLLLDR 236          

HSP 12 Score: 53.9138 bits (128), Expect = 1.325e-8
Identity = 28/88 (31.82%), Postives = 43/88 (48.86%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  PIH+A +   +D  R LL  N  ID        P+H A+  GH  + K+LLD+         +G+ P+H A    H+  + +LL
Sbjct:  279 NGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLL 366          

HSP 13 Score: 53.5286 bits (127), Expect = 1.730e-8
Identity = 27/88 (30.68%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  +    LLL ++ +++   +    P+H A+  GH  I+K LL R  +          P+H A+  GH E+ + LL
Sbjct:  345 NGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKSLLQRGASPNASNVKVETPLHMAARAGHCEVAQFLL 432          

HSP 14 Score: 53.5286 bits (127), Expect = 1.747e-8
Identity = 23/86 (26.74%), Postives = 48/86 (55.81%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+  +H+AC  G + + + LL    +++C+ +  + P+H A++ GH++I+ LLL         T +G  P+  A   G++ ++ +L
Sbjct:  681 GYTALHVACHYGNIKMVKFLLQQQAHVNCKTRVGYTPLHQAAQQGHTDIVTLLLKHGALPNEITTNGTSPLGIAKRLGYISVIDVL 766          

HSP 15 Score: 49.6766 bits (117), Expect = 3.238e-7
Identity = 24/103 (23.30%), Postives = 54/103 (52.43%), Query Frame = 1
Query:  190 ENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +++K        + +G   +H+A +EG V +   LL +  +++   K+    +H A+  G  +++  L+    N   +++ G+ P++ A+   HLE++K LL+
Sbjct:    5 DHIKNGIAINTANQNGLNGLHLASKEGHVKMVLELLHHGIDLETTTKKGNTALHIAALAGQEKVVAELVHYGANVNAQSQKGFSPLYMAAQENHLEVVKFLLE 107          

HSP 16 Score: 47.7506 bits (112), Expect = 1.769e-6
Identity = 25/95 (26.32%), Postives = 50/95 (52.63%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETK-----SGWRPVHWASSGGHLEILKILLDR 501
            +G+ P+HIA ++  ++VA  LL    N + E+ +   P+H A++ G  +++ LL+ +  N     K     +   P+H  +  GH+ I  +L+ +
Sbjct:  576 NGYTPLHIAAKQNQLEVASSLLQCGANANSESLQGITPLHLAAQEGRPDMVALLISKQGNVNLGNKVRTQLTALTPLHLVAQEGHVGIADMLVKQ 670          

HSP 17 Score: 46.9802 bits (110), Expect = 2.977e-6
Identity = 23/90 (25.56%), Postives = 45/90 (50.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A     +DV +LL+    +    A+  + P+H A++    E+   LL    N   E+  G  P+H A+  G  +++ +L+ +
Sbjct:  543 NGLTPLHVAVHHNNLDVVKLLVSKGGSPHSTARNGYTPLHIAAKQNQLEVASSLLQCGANANSESLQGITPLHLAAQEGRPDMVALLISK 632          
BLAST of SMED30027856 vs. Ensembl Cavefish
Match: ank3b (ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2])

HSP 1 Score: 77.7962 bits (190), Expect = 6.981e-17
Identity = 35/89 (39.33%), Postives = 57/89 (64.04%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  P+HIA   G ++VA LLL+    +D +A+    P+H AS+ G+S ++KLLL+R    + +TK G  P+H  +  GH +++++LLDR
Sbjct:  182 GFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDR 270          

HSP 2 Score: 72.0182 bits (175), Expect = 6.300e-15
Identity = 30/84 (35.71%), Postives = 52/84 (61.90%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            P+H+A + G  ++ +LLL+    ID + K    P+H  +R GH +++++LLDR      +TK+G  P+H A+ G HL  +++LL
Sbjct:  218 PLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLL 301          

HSP 3 Score: 68.1662 bits (165), Expect = 1.605e-13
Identity = 38/115 (33.04%), Postives = 59/115 (51.30%), Query Frame = 1
Query:  157 QLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            QLMN  A     N++GE             +H+A R G  +V + L+ N   +D ++K    P+H +SR G  EI++ LL      +  T SG+ P+H A+  GH ++  ILLD+
Sbjct:  398 QLMNHGASPNTTNVRGETA-----------LHMAARAGQTEVVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQ 501          

HSP 4 Score: 64.3142 bits (155), Expect = 3.768e-12
Identity = 32/100 (32.00%), Postives = 54/100 (54.00%), Query Frame = 1
Query:  202 GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G +VD    DD   P+HI+ R G  ++ + LL +    D      + P+H A+R GH ++  +LLD+  +    TK G+ P+H A+  G +E+  +LL +
Sbjct:  436 GAYVDAKSKDD-QTPLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQK 534          

HSP 5 Score: 63.929 bits (154), Expect = 4.835e-12
Identity = 31/89 (34.83%), Postives = 52/89 (58.43%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G+ P+H+A REG  DVA +LLD   ++    K+ + P+H A++ G  E+  LLL +    +   KSG  P+H A+   + ++  +LLD+
Sbjct:  479 GYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQ 567          

HSP 6 Score: 58.9214 bits (141), Expect = 2.357e-10
Identity = 26/93 (27.96%), Postives = 53/93 (56.99%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            + + +G   +H+A +EG V+V   L+     +D   K+    +H AS  G S+++K L+    N   ++++G+ P++ A+   HL++++ LLD
Sbjct:   16 ICNQNGLNALHLASKEGHVEVVTQLIKMGATVDAATKKGNTALHIASLAGQSDVVKELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRYLLD 108          

HSP 7 Score: 56.225 bits (134), Expect = 1.891e-9
Identity = 30/94 (31.91%), Postives = 54/94 (57.45%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +  +DG  P+ +A ++G   V  LLL+N    D + K +   +H A+R   ++   LLL  + N + E+KSG+ P+H A+  G++ +  +LL+R
Sbjct:  115 IATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNR 204          

HSP 8 Score: 56.225 bits (134), Expect = 2.081e-9
Identity = 28/88 (31.82%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G  P+H+A + G ++VA LLL      D   K    P+H A+   + ++  LLLD+  +     K+G+ P+H A+    +EI   LL+
Sbjct:  512 GFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLLE 599          

HSP 9 Score: 53.9138 bits (128), Expect = 1.428e-8
Identity = 31/111 (27.93%), Postives = 61/111 (54.95%), Query Frame = 1
Query:  169 GNAEGVRENLK-GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G+ E V + +K G  VD      G+  +HIA   G  DV + L+ N  N++ +++  + P++ A++  H ++++ LLD   +    T+ G+ P+  A   GH +++ +LL+
Sbjct:   32 GHVEVVTQLIKMGATVDAATKK-GNTALHIASLAGQSDVVKELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRYLLDNGSSQSIATEDGFTPLAVALQQGHDQVVSLLLE 141          

HSP 10 Score: 53.1434 bits (126), Expect = 2.729e-8
Identity = 27/91 (29.67%), Postives = 50/91 (54.95%), Query Frame = 1
Query:  223 IDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            I   G  P+H+A +EG VD+  LL+  +  I+   K    P+H A++     + ++L++     + ETK G+ P+H A   G+++++  LL
Sbjct:  607 ITRQGISPLHLASQEGNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVNHGATVDPETKMGYTPLHVACHYGNVKMVHFLL 697          

HSP 11 Score: 52.7582 bits (125), Expect = 3.001e-8
Identity = 23/89 (25.84%), Postives = 52/89 (58.43%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +G+ P+HIA ++  +++A  LL+   + +   ++   P+H AS+ G+ +++ LL+ R+       KSG  P+H A+    + + ++L++
Sbjct:  577 NGYTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVN 665          

HSP 12 Score: 52.7582 bits (125), Expect = 3.208e-8
Identity = 28/89 (31.46%), Postives = 46/89 (51.69%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  P+H+A       VA LLLD   +    AK  + P+H A++    EI   LL+   +    T+ G  P+H AS  G+++++ +L+ R
Sbjct:  545 GLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMAR 633          

HSP 13 Score: 52.373 bits (124), Expect = 4.562e-8
Identity = 27/90 (30.00%), Postives = 44/90 (48.89%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            DG  P+H   R G   V  +LLD    I  + K    P+H A++G H   ++LLL      +  T      +H A+  GH ++ K+++D+
Sbjct:  247 DGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDK 336          

HSP 14 Score: 51.6026 bits (122), Expect = 8.879e-8
Identity = 24/84 (28.57%), Postives = 47/84 (55.95%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +H+A   G   VA++++D   N + +A   + P+H A +    ++++LLL    + +  T+SG  P+H A+  GH  I+  L++
Sbjct:  318 LHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLMN 401          

HSP 15 Score: 51.2174 bits (121), Expect = 9.857e-8
Identity = 26/88 (29.55%), Postives = 44/88 (50.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LLL +  +I    +    PIH A+  GH  I+  L++   +       G   +H A+  G  E++K L+
Sbjct:  346 NGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLMNHGASPNTTNVRGETALHMAARAGQTEVVKYLV 433          

HSP 16 Score: 48.1358 bits (113), Expect = 1.328e-6
Identity = 29/109 (26.61%), Postives = 53/109 (48.62%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            GN + V   +  +    + +  G  P+H+A +E  V+VA +L+++   +D E K  + P+H A   G+ +++  LL        +TK        A+  GH  I+ +LL
Sbjct:  622 GNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVNHGATVDPETKMGYTPLHVACHYGNVKMVHFLLKNQAKVNAKTKVT------AAQQGHTHIINLLL 724          

HSP 17 Score: 45.8246 bits (107), Expect = 7.365e-6
Identity = 21/88 (23.86%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +   ++  +LLL +   +D         +H A+  GH ++ K+++D+  N   +  +G+ P+H A     ++++++LL
Sbjct:  280 NGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRIKVMELLL 367          
BLAST of SMED30027856 vs. Ensembl Cavefish
Match: ENSAMXT00000017800.2 (pep primary_assembly:Astyanax_mexicanus-2.0:16:21012912:21225385:-1 gene:ENSAMXG00000017269.2 transcript:ENSAMXT00000017800.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 76.6406 bits (187), Expect = 1.868e-16
Identity = 37/99 (37.37%), Postives = 56/99 (56.57%), Query Frame = 1
Query:  199 KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  VD     +G  P+H+A + G  ++ RLLLD    ID + +    P+H A+R GH   ++LLL+R       TK+G  P+H A+ G H+E +K LL
Sbjct:  248 RGAAVDFTARQNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPILARTKNGLSPLHMAAQGDHVECVKHLL 346          

HSP 2 Score: 69.3218 bits (168), Expect = 5.756e-14
Identity = 34/90 (37.78%), Postives = 56/90 (62.22%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKW-RPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  P+HIA   G V+VA LLL+    +D  A++    P+H AS+ G++ +++LLLDR    + +T+ G  P+H A+  GH   +++LL+R
Sbjct:  226 GFTPLHIAAHYGNVNVATLLLNRGAAVDFTARQNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHDTAVELLLER 315          

HSP 3 Score: 67.781 bits (164), Expect = 1.949e-13
Identity = 40/123 (32.52%), Postives = 63/123 (51.22%), Query Frame = 1
Query:  130 MGEFECFKEQLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            MG        L NG +  V  N++GE             +H+A R G V+V R LL N   +D  A+    P+H ASR G +EI++LLL    + +  T +G+ P+H ++  G L++  +LL+
Sbjct:  437 MGHLNIVLLLLQNGASPDV-SNIRGETA-----------LHMAARAGQVEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLDVASVLLE 547          

HSP 4 Score: 67.781 bits (164), Expect = 1.968e-13
Identity = 32/110 (29.09%), Postives = 65/110 (59.09%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            GN + V E LKG       + +G   +H+A +EG +D+ + LL+   ++D   K+    +H AS  G +E++K+L+ +  +   ++++G+ P++ AS   HL++++ LL+
Sbjct:   43 GNIDKVLEYLKGGVDIGTSNQNGLNALHLAAKEGHMDLVQELLERGSSVDSATKKGNTALHIASLAGQAEVVKILVKQGADINAQSQNGFTPLYMASQENHLDVVRYLLE 152          

HSP 5 Score: 64.6994 bits (156), Expect = 2.186e-12
Identity = 38/112 (33.93%), Postives = 60/112 (53.57%), Query Frame = 1
Query:  169 GNAEGVRENLK-GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  E VR  L+ G  VD    +D   P+HIA R G  ++ +LLL +  + D      + P+H ++R G  ++  +LL+   +    TK G+ P+H AS  G L++ K+LL R
Sbjct:  471 GQVEVVRCLLRNGAMVDARARED-QTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLDVASVLLEAGASHSLATKKGFTPLHVASKYGSLDVAKLLLQR 581          

HSP 6 Score: 62.003 bits (149), Expect = 2.138e-11
Identity = 31/90 (34.44%), Postives = 54/90 (60.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+ P+HI+ REG +DVA +LL+   +     K+ + P+H AS+ G  ++ KLLL R    +   K+G  P+H A+   + ++  +LLD+
Sbjct:  525 NGYTPLHISAREGQLDVASVLLEAGASHSLATKKGFTPLHVASKYGSLDVAKLLLQRRAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDK 614          

HSP 7 Score: 57.7658 bits (138), Expect = 6.022e-10
Identity = 29/87 (33.33%), Postives = 46/87 (52.87%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G  P+H+A + G +DVA+LLL      D   K    P+H A+   + ++  LLLD+  +     K+G+ P+H A+    +EI   LL
Sbjct:  559 GFTPLHVASKYGSLDVAKLLLQRRAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTLL 645          

HSP 8 Score: 56.6102 bits (135), Expect = 1.641e-9
Identity = 26/83 (31.33%), Postives = 50/83 (60.24%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A +E  V VA +L+ N  ++D + K  + P+  A   G+++++  LL  N +   +TK+G+ P+H A+  G+  I+ +LL
Sbjct:  695 LHLAAQEDRVGVAEILVKNGASMDQQTKLGYTPLIVACHYGNAKMVNFLLKSNASVNAKTKNGYTPLHQAAQQGNTHIINVLL 777          

HSP 9 Score: 56.225 bits (134), Expect = 1.876e-9
Identity = 30/86 (34.88%), Postives = 50/86 (58.14%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETK-SGWRPVHWASSGGHLEILKILLDR 501
            +HIA R+     A LLL N+ N D ++K  + P+H A+  G+  +  LLL+R    +   + +G  P+H AS  G+  ++++LLDR
Sbjct:  197 LHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARQNGITPLHVASKRGNTNMVRLLLDR 282          

HSP 10 Score: 53.9138 bits (128), Expect = 1.181e-8
Identity = 27/88 (30.68%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G+ P+HIA ++  +++A  LL      +   K+   P+H AS+ GHS++  LLL +       TKSG   +H A+    + + +IL+
Sbjct:  624 NGYTPLHIAAKKNQMEIATTLLQYEAETNILTKQGVTPLHLASQEGHSDMAALLLQKGAQVNVPTKSGLTALHLAAQEDRVGVAEILV 711          

HSP 11 Score: 53.9138 bits (128), Expect = 1.227e-8
Identity = 29/91 (31.87%), Postives = 53/91 (58.24%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +DG  P+ IA ++G   V  +LL+N    D + K +   +H A+R   ++   LLL  + N + ++KSG+ P+H A+  G++ +  +LL+R
Sbjct:  162 EDGFTPLAIALQQGHNQVVSILLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNR 248          

HSP 12 Score: 53.9138 bits (128), Expect = 1.443e-8
Identity = 31/111 (27.93%), Postives = 62/111 (55.86%), Query Frame = 1
Query:  169 GNAEGVRENL-KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G+ + V+E L +G  VD      G+  +HIA   G  +V ++L+    +I+ +++  + P++ AS+  H ++++ LL+   N    T+ G+ P+  A   GH +++ ILL+
Sbjct:   76 GHMDLVQELLERGSSVDSATKK-GNTALHIASLAGQAEVVKILVKQGADINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLE 185          

HSP 13 Score: 53.1434 bits (126), Expect = 2.811e-8
Identity = 30/90 (33.33%), Postives = 43/90 (47.78%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A       VA LLLD   +    AK  + P+H A++    EI   LL         TK G  P+H AS  GH ++  +LL +
Sbjct:  591 NGLTPLHVAAHYDNQKVALLLLDKGASPHATAKNGYTPLHIAAKKNQMEIATTLLQYEAETNILTKQGVTPLHLASQEGHSDMAALLLQK 680          

HSP 14 Score: 51.9878 bits (123), Expect = 5.848e-8
Identity = 28/88 (31.82%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  V V  LL+    +I    +    PIH A+  GH  I+ LLL    + +     G   +H A+  G +E+++ LL
Sbjct:  393 NGFTPLHIACKKNRVKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETALHMAARAGQVEVVRCLL 480          

HSP 15 Score: 51.2174 bits (121), Expect = 1.116e-7
Identity = 27/85 (31.76%), Postives = 47/85 (55.29%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEA--KRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A   G   V +LLLD   N +  A  +  + P+H A +    ++++LL+    + +  T+SG  P+H A+  GHL I+ +LL
Sbjct:  363 LHVAAHCGHYRVTKLLLDKRANPNARALLQNGFTPLHIACKKNRVKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL 447          
BLAST of SMED30027856 vs. Ensembl Cavefish
Match: ENSAMXT00000037173.1 (pep primary_assembly:Astyanax_mexicanus-2.0:16:21074007:21119253:-1 gene:ENSAMXG00000017269.2 transcript:ENSAMXT00000037173.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 75.485 bits (184), Expect = 3.417e-16
Identity = 35/94 (37.23%), Postives = 56/94 (59.57%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            V    G  P+HIA   G V+VA LLL+    +D  A+    P+H AS+ G++ +++LLLDR    + +T+ G  P+H A+  GH   +++LL+R
Sbjct:  204 VQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHDTAVELLLER 297          

HSP 2 Score: 74.7146 bits (182), Expect = 7.070e-16
Identity = 34/88 (38.64%), Postives = 52/88 (59.09%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A + G  ++ RLLLD    ID + +    P+H A+R GH   ++LLL+R       TK+G  P+H A+ G H+E +K LL
Sbjct:  241 NGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPILARTKNGLSPLHMAAQGDHVECVKHLL 328          

HSP 3 Score: 70.4774 bits (171), Expect = 2.126e-14
Identity = 32/110 (29.09%), Postives = 65/110 (59.09%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            GN + V E LKG       + +G   +H+A +EG +D+ + LL+   ++D   K+    +H AS  G +E++K+L+ +  +   ++++G+ P++ AS   HL++++ LL+
Sbjct:   26 GNIDKVLEYLKGGVDIGTSNQNGLNALHLAAKEGHMDLVQELLERGSSVDSATKKGNTALHIASLAGQAEVVKILVKQGADINAQSQNGFTPLYMASQENHLDVVRYLLE 135          

HSP 4 Score: 63.929 bits (154), Expect = 4.031e-12
Identity = 30/85 (35.29%), Postives = 50/85 (58.82%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R+     A LLL N+ N D ++K  + P+H A+  G+  +  LLL+R    +   ++G  P+H AS  G+  ++++LLDR
Sbjct:  180 LHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDR 264          

HSP 5 Score: 57.7658 bits (138), Expect = 5.449e-10
Identity = 31/111 (27.93%), Postives = 62/111 (55.86%), Query Frame = 1
Query:  169 GNAEGVRENL-KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G+ + V+E L +G  VD      G+  +HIA   G  +V ++L+    +I+ +++  + P++ AS+  H ++++ LL+   N    T+ G+ P+  A   GH +++ ILL+
Sbjct:   59 GHMDLVQELLERGSSVDSATKK-GNTALHIASLAGQAEVVKILVKQGADINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLE 168          

HSP 6 Score: 56.9954 bits (136), Expect = 8.875e-10
Identity = 29/91 (31.87%), Postives = 53/91 (58.24%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +DG  P+ IA ++G   V  +LL+N    D + K +   +H A+R   ++   LLL  + N + ++KSG+ P+H A+  G++ +  +LL+R
Sbjct:  145 EDGFTPLAIALQQGHNQVVSILLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNR 231          

HSP 7 Score: 56.6102 bits (135), Expect = 1.116e-9
Identity = 27/83 (32.53%), Postives = 46/83 (55.42%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A   G   V +LLLD   N +  A   + P+H A +    ++++LL+    + +  T+SG  P+H A+  GHL I+ +LL
Sbjct:  345 LHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLL 427          

HSP 8 Score: 46.595 bits (109), Expect = 3.485e-6
Identity = 23/88 (26.14%), Postives = 45/88 (51.14%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +   V+  + LL +   +D         +H A+  GH  + KLLLD+  N      +G+ P+H A     ++++++L+
Sbjct:  307 NGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLV 394          
BLAST of SMED30027856 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000095.1 (pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000095.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 74.7146 bits (182), Expect = 1.366e-16
Identity = 32/90 (35.56%), Postives = 57/90 (63.33%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A + G  ++ +LLLD    ID + +    P+H A+R GH +++++LL+R+     +TK+G  P+H A+ G HL  +++LL R
Sbjct:  237 NGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGR 326          

HSP 2 Score: 73.9442 bits (180), Expect = 2.977e-16
Identity = 36/102 (35.29%), Postives = 61/102 (59.80%), Query Frame = 1
Query:  196 LKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            L+ E    V    G  P+HIA   G ++VA LLL+    +D  A+    P+H A++ G++ ++KLLLDR    + +T+ G  P+H A+  GH +++++LL+R
Sbjct:  192 LQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLER 293          

HSP 3 Score: 72.7886 bits (177), Expect = 7.283e-16
Identity = 37/111 (33.33%), Postives = 63/111 (56.76%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            NGN E + ++LKG       + +G   +H+A +EG VDV   LL     ID   K     +H A+  G ++I+++L++   N   ++++G+ P++ A+   HLEI+K LLD
Sbjct:   21 NGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLD 131          

HSP 4 Score: 67.3958 bits (163), Expect = 5.497e-14
Identity = 41/126 (32.54%), Postives = 63/126 (50.00%), Query Frame = 1
Query:  130 MGEFECFKEQLMNG---NAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            MG     +  L NG   NA  VR               G  P+H+A R G ++V + LL +   +  +A+    P+H ASR G +EI++LLL R  +    T SG  P+++AS  GH ++  +LL+
Sbjct:  413 MGHLNVVQLLLQNGASSNATNVR---------------GETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLE 523          

HSP 5 Score: 65.4698 bits (158), Expect = 2.480e-13
Identity = 37/123 (30.08%), Postives = 60/123 (48.78%), Query Frame = 1
Query:  133 GEFECFKEQLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G+ E  K  L +G   GV+              +   P+HIA R G  ++ +LLL    + +        P+++ASR GH ++  LLL+   +    TK G+ P+H AS  G LE+  +LL++
Sbjct:  447 GQLEVVKTLLQSGARVGVKAR------------EDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEK 557          

HSP 6 Score: 63.5438 bits (153), Expect = 1.328e-12
Identity = 32/88 (36.36%), Postives = 47/88 (53.41%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LLL +  +I    +    PIH AS  GH  +++LLL    +       G  P+H AS  G LE++K LL
Sbjct:  369 NGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLL 456          

HSP 7 Score: 62.3882 bits (150), Expect = 3.364e-12
Identity = 34/110 (30.91%), Postives = 60/110 (54.55%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G+ + V E LK E         G+  +HIA   G  D+ R+L++   NI+ +++  + P++ A++  H EI+K LLD   N    T+ G+ P+  A   GH +++ +LL+
Sbjct:   55 GHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLE 164          

HSP 8 Score: 60.077 bits (144), Expect = 1.780e-11
Identity = 32/91 (35.16%), Postives = 48/91 (52.75%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            + G  PIH+A   G ++V +LLL N  + +    R   P+H ASR G  E++K LL        + +    P+H AS  G  EI+++LL R
Sbjct:  401 ESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQR 491          

HSP 9 Score: 59.6918 bits (143), Expect = 2.635e-11
Identity = 29/84 (34.52%), Postives = 47/84 (55.95%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            P+H+A   G   VA+LLLD     +  A   + P+H A +    ++++LLL    +    T+SG  P+H AS  GHL ++++LL
Sbjct:  340 PLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLL 423          

HSP 10 Score: 56.9954 bits (136), Expect = 2.264e-10
Identity = 30/94 (31.91%), Postives = 51/94 (54.26%), Query Frame = 1
Query:  223 IDDDGHFPIHIACREGFVDVARLLLDNNCNID--CEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +   G  P+HIA + G ++VA LLL+   + D     +    P+H A+   H ++  LLLD+  +     K+G+ P+H A+    LE+ K+LL+
Sbjct:  531 VTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLE 624          

HSP 11 Score: 56.6102 bits (135), Expect = 2.564e-10
Identity = 31/95 (32.63%), Postives = 49/95 (51.58%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            KV   +G  P+H+A       VA LLLD   +    AK  + P+H A++    E+ K+LL+        T+ G  P+H A+  GH +++ +LL R
Sbjct:  564 KVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGR 658          

HSP 12 Score: 54.299 bits (129), Expect = 1.796e-9
Identity = 28/85 (32.94%), Postives = 48/85 (56.47%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R+   + A LLL +  N D  +K  + P+H A+  G+  +  LLL+     +   ++G  P+H A+  G+  ++K+LLDR
Sbjct:  176 LHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDR 260          

HSP 13 Score: 52.7582 bits (125), Expect = 5.854e-9
Identity = 24/87 (27.59%), Postives = 51/87 (58.62%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            +G+ P+HIA ++  ++V ++LL+     +   ++   P+H A++ GH++++ LLL R+ +    TK G   +H A+    + + +IL
Sbjct:  602 NGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEIL 688          

HSP 14 Score: 52.373 bits (124), Expect = 7.428e-9
Identity = 24/83 (28.92%), Postives = 47/83 (56.63%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A +E  V+VA +L  +  +++   K  + P+H AS  G+ +++  LL        +T++G+ P+H A+  GH  ++  LL
Sbjct:  673 MHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALL 755          

HSP 15 Score: 48.9062 bits (115), Expect = 1.299e-7
Identity = 28/93 (30.11%), Postives = 50/93 (53.76%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            V  +DG  P+ +A ++G   V  LLL++    D + K +   +H A+R   +    LLL    N +  +KSG+ P+H A+  G++ +  +LL+
Sbjct:  138 VATEDGFTPLAVALQQGHEQVVGLLLES----DTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLN 226          

HSP 16 Score: 47.3654 bits (111), Expect = 4.760e-7
Identity = 25/88 (28.41%), Postives = 44/88 (50.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +   +   +LLL     +D        P+H A+  GH  + KLLLDR         +G+ P+H A     ++++++LL
Sbjct:  303 NGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLL 390          

HSP 17 Score: 45.0542 bits (105), Expect = 2.614e-6
Identity = 24/92 (26.09%), Postives = 46/92 (50.00%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            ++   G  P+H+A +EG  D+  LLL  + +I    K     +H A++     + ++L     +    TK  + P+H AS  G+++++  LL
Sbjct:  631 MLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLL 722          
BLAST of SMED30027856 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000093.1 (pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000093.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 74.7146 bits (182), Expect = 1.378e-16
Identity = 32/90 (35.56%), Postives = 57/90 (63.33%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A + G  ++ +LLLD    ID + +    P+H A+R GH +++++LL+R+     +TK+G  P+H A+ G HL  +++LL R
Sbjct:  244 NGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGR 333          

HSP 2 Score: 73.1738 bits (178), Expect = 5.834e-16
Identity = 33/91 (36.26%), Postives = 58/91 (63.74%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            + G  P+HIA   G ++VA LLL+    +D  A+    P+H A++ G++ ++KLLLDR    + +T+ G  P+H A+  GH +++++LL+R
Sbjct:  210 ESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLER 300          

HSP 3 Score: 72.7886 bits (177), Expect = 7.348e-16
Identity = 37/111 (33.33%), Postives = 63/111 (56.76%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            NGN E + ++LKG       + +G   +H+A +EG VDV   LL     ID   K     +H A+  G ++I+++L++   N   ++++G+ P++ A+   HLEI+K LLD
Sbjct:   20 NGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLD 130          

HSP 4 Score: 67.3958 bits (163), Expect = 5.546e-14
Identity = 41/126 (32.54%), Postives = 63/126 (50.00%), Query Frame = 1
Query:  130 MGEFECFKEQLMNG---NAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            MG     +  L NG   NA  VR               G  P+H+A R G ++V + LL +   +  +A+    P+H ASR G +EI++LLL R  +    T SG  P+++AS  GH ++  +LL+
Sbjct:  420 MGHLNVVQLLLQNGASSNATNVR---------------GETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLE 530          

HSP 5 Score: 65.4698 bits (158), Expect = 2.502e-13
Identity = 37/123 (30.08%), Postives = 60/123 (48.78%), Query Frame = 1
Query:  133 GEFECFKEQLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G+ E  K  L +G   GV+              +   P+HIA R G  ++ +LLL    + +        P+++ASR GH ++  LLL+   +    TK G+ P+H AS  G LE+  +LL++
Sbjct:  454 GQLEVVKTLLQSGARVGVKAR------------EDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEK 564          

HSP 6 Score: 65.4698 bits (158), Expect = 2.781e-13
Identity = 33/89 (37.08%), Postives = 54/89 (60.67%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  P++ A REG  DVA LLL+N  +I    K+ + P+H AS+ G  E+  LLL++  + + + K+G  P+H A+   H ++  +LLD+
Sbjct:  509 GSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDK 597          

HSP 7 Score: 63.5438 bits (153), Expect = 1.340e-12
Identity = 32/88 (36.36%), Postives = 47/88 (53.41%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LLL +  +I    +    PIH AS  GH  +++LLL    +       G  P+H AS  G LE++K LL
Sbjct:  376 NGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLL 463          

HSP 8 Score: 63.1586 bits (152), Expect = 1.639e-12
Identity = 31/92 (33.70%), Postives = 52/92 (56.52%), Query Frame = 1
Query:  223 IDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +   G  P+HIA + G ++VA LLL+   + D + K    P+H A+   H ++  LLLD+  +     K+G+ P+H A+    LE+ K+LL+
Sbjct:  538 VTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLE 629          

HSP 9 Score: 62.3882 bits (150), Expect = 3.394e-12
Identity = 34/110 (30.91%), Postives = 60/110 (54.55%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G+ + V E LK E         G+  +HIA   G  D+ R+L++   NI+ +++  + P++ A++  H EI+K LLD   N    T+ G+ P+  A   GH +++ +LL+
Sbjct:   54 GHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLE 163          

HSP 10 Score: 60.077 bits (144), Expect = 1.796e-11
Identity = 32/91 (35.16%), Postives = 48/91 (52.75%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            + G  PIH+A   G ++V +LLL N  + +    R   P+H ASR G  E++K LL        + +    P+H AS  G  EI+++LL R
Sbjct:  408 ESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQR 498          

HSP 11 Score: 59.6918 bits (143), Expect = 2.658e-11
Identity = 29/84 (34.52%), Postives = 47/84 (55.95%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            P+H+A   G   VA+LLLD     +  A   + P+H A +    ++++LLL    +    T+SG  P+H AS  GHL ++++LL
Sbjct:  347 PLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLL 430          

HSP 12 Score: 54.6842 bits (130), Expect = 1.526e-9
Identity = 29/90 (32.22%), Postives = 47/90 (52.22%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A       VA LLLD   +    AK  + P+H A++    E+ K+LL+        T+ G  P+H A+  GH +++ +LL R
Sbjct:  574 NGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGR 663          

HSP 13 Score: 52.7582 bits (125), Expect = 5.904e-9
Identity = 24/87 (27.59%), Postives = 51/87 (58.62%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            +G+ P+HIA ++  ++V ++LL+     +   ++   P+H A++ GH++++ LLL R+ +    TK G   +H A+    + + +IL
Sbjct:  607 NGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEIL 693          

HSP 14 Score: 52.373 bits (124), Expect = 7.491e-9
Identity = 24/83 (28.92%), Postives = 47/83 (56.63%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A +E  V+VA +L  +  +++   K  + P+H AS  G+ +++  LL        +T++G+ P+H A+  GH  ++  LL
Sbjct:  678 MHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALL 760          

HSP 15 Score: 47.3654 bits (111), Expect = 4.799e-7
Identity = 25/88 (28.41%), Postives = 44/88 (50.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +   +   +LLL     +D        P+H A+  GH  + KLLLDR         +G+ P+H A     ++++++LL
Sbjct:  310 NGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLL 397          

HSP 16 Score: 45.0542 bits (105), Expect = 2.634e-6
Identity = 24/92 (26.09%), Postives = 46/92 (50.00%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            ++   G  P+H+A +EG  D+  LLL  + +I    K     +H A++     + ++L     +    TK  + P+H AS  G+++++  LL
Sbjct:  636 MLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLL 727          
BLAST of SMED30027856 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007449.1 (pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007449.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 69.3218 bits (168), Expect = 1.216e-14
Identity = 38/112 (33.93%), Postives = 61/112 (54.46%), Query Frame = 1
Query:  169 GNAEGVRENLK-GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  E VR  L+ G  VD    ++   P+HIA R G +++ +LLL +  + D      + P+H A+R GH ++  +LL+   +    TK G+ P+H AS  G +E+  +LL R
Sbjct:   92 GQTEVVRSLLRNGAQVDAKAREE-QTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQR 202          

HSP 2 Score: 67.3958 bits (163), Expect = 6.220e-14
Identity = 37/113 (32.74%), Postives = 60/113 (53.10%), Query Frame = 1
Query:  160 LMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            L+ G A     N++GE             +H+A R G  +V R LL N   +D +A+ +  P+H A+R G  EI++LLL    + +  T +G+ P+H A+  GH ++  ILL+
Sbjct:   67 LLQGGASPNATNVRGETA-----------LHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLE 168          

HSP 3 Score: 63.5438 bits (153), Expect = 1.084e-12
Identity = 31/90 (34.44%), Postives = 54/90 (60.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+ P+H+A REG  DV  +LL++  ++    K+ + P+H AS+ G  E+  LLL R  + +   K+G  P+H AS   + ++  +LLD+
Sbjct:  146 NGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQ 235          

HSP 4 Score: 56.6102 bits (135), Expect = 2.897e-10
Identity = 31/93 (33.33%), Postives = 48/93 (51.61%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            V    G  P+H+A + G ++VA LLL    + D   K    P+H AS   + ++  LLLD+  +     K+G+ P+H A+    LEI   LL+
Sbjct:  175 VTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLE 267          

HSP 5 Score: 55.8398 bits (133), Expect = 5.387e-10
Identity = 26/87 (29.89%), Postives = 50/87 (57.47%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G  P+H+A +E  V+VA +L+ +  +++   K  + P+  A   G+ +++  LL    +   +TK+G+ P+H A+  GH  I+ +LL
Sbjct:  312 GLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLL 398          

HSP 6 Score: 54.299 bits (129), Expect = 1.676e-9
Identity = 25/88 (28.41%), Postives = 50/88 (56.82%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G+ P+HIA ++  +++A  LL+     +   ++   P+H  ++ GH+++  LLL R  +    TKSG  P+H A+    + + +IL+
Sbjct:  245 NGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILV 332          

HSP 7 Score: 51.2174 bits (121), Expect = 2.256e-8
Identity = 28/90 (31.11%), Postives = 43/90 (47.78%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A       VA LLLD   +    AK  + P+H A++    EI   LL+        T+ G  P+H  +  GH ++  +LL R
Sbjct:  212 NGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLAR 301          

HSP 8 Score: 48.1358 bits (113), Expect = 2.588e-7
Identity = 23/93 (24.73%), Postives = 49/93 (52.69%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            + + + G  P+H++   G +++  +LL    + +    R    +H A+R G +E+++ LL      + + +    P+H A+  G LEI+++LL
Sbjct:   42 EAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLL 134          

HSP 9 Score: 46.9802 bits (110), Expect = 5.119e-7
Identity = 25/91 (27.47%), Postives = 46/91 (50.55%), Query Frame = 1
Query:  223 IDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            I   G  P+H+  +EG  DVA LLL    +++   K    P+H A++     + ++L+    +    TK G+ P+  A   G+++++  LL
Sbjct:  275 ITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLL 365          

HSP 10 Score: 44.2838 bits (103), Expect = 4.419e-6
Identity = 24/88 (27.27%), Postives = 44/88 (50.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LLL    +I+   +    P+H ++  G+  I+ +LL    +       G   +H A+  G  E+++ LL
Sbjct:   14 NGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLL 101          

HSP 11 Score: 43.8986 bits (102), Expect = 5.865e-6
Identity = 21/70 (30.00%), Postives = 37/70 (52.86%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRP 444
            G+ P+ +AC  G + +   LL +  +++ + K  + P+H A++ GH+ I+ LLL          K G RP
Sbjct:  345 GYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLL----------KCGARP 404          

HSP 12 Score: 43.5134 bits (101), Expect = 9.486e-6
Identity = 23/68 (33.82%), Postives = 37/68 (54.41%), Query Frame = 1
Query:  292 LLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            LLD   N +  A   + P+H A +     +++LLL    + E  T+SG  P+H ++  G+L I+ ILL
Sbjct:    1 LLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILL 68          
BLAST of SMED30027856 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007446.1 (pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007446.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 69.3218 bits (168), Expect = 1.245e-14
Identity = 38/112 (33.93%), Postives = 61/112 (54.46%), Query Frame = 1
Query:  169 GNAEGVRENLK-GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  E VR  L+ G  VD    ++   P+HIA R G +++ +LLL +  + D      + P+H A+R GH ++  +LL+   +    TK G+ P+H AS  G +E+  +LL R
Sbjct:  182 GQTEVVRSLLRNGAQVDAKAREE-QTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQR 292          

HSP 2 Score: 67.3958 bits (163), Expect = 6.369e-14
Identity = 37/113 (32.74%), Postives = 60/113 (53.10%), Query Frame = 1
Query:  160 LMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            L+ G A     N++GE             +H+A R G  +V R LL N   +D +A+ +  P+H A+R G  EI++LLL    + +  T +G+ P+H A+  GH ++  ILL+
Sbjct:  157 LLQGGASPNATNVRGETA-----------LHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLE 258          

HSP 3 Score: 63.5438 bits (153), Expect = 1.110e-12
Identity = 31/90 (34.44%), Postives = 54/90 (60.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+ P+H+A REG  DV  +LL++  ++    K+ + P+H AS+ G  E+  LLL R  + +   K+G  P+H AS   + ++  +LLD+
Sbjct:  236 NGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQ 325          

HSP 4 Score: 56.6102 bits (135), Expect = 2.964e-10
Identity = 31/93 (33.33%), Postives = 48/93 (51.61%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            V    G  P+H+A + G ++VA LLL    + D   K    P+H AS   + ++  LLLD+  +     K+G+ P+H A+    LEI   LL+
Sbjct:  265 VTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLE 357          

HSP 5 Score: 55.8398 bits (133), Expect = 5.512e-10
Identity = 26/87 (29.89%), Postives = 50/87 (57.47%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G  P+H+A +E  V+VA +L+ +  +++   K  + P+  A   G+ +++  LL    +   +TK+G+ P+H A+  GH  I+ +LL
Sbjct:  402 GLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLL 488          

HSP 6 Score: 54.299 bits (129), Expect = 1.715e-9
Identity = 25/88 (28.41%), Postives = 50/88 (56.82%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G+ P+HIA ++  +++A  LL+     +   ++   P+H  ++ GH+++  LLL R  +    TKSG  P+H A+    + + +IL+
Sbjct:  335 NGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILV 422          

HSP 7 Score: 51.6026 bits (122), Expect = 1.367e-8
Identity = 28/90 (31.11%), Postives = 44/90 (48.89%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            DG  P+H A R G   V  +LL+    I  + K    P+H A++G H E +++L+      +  T      +H A+  GH    K+LLD+
Sbjct:    5 DGLTPLHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDK 94          

HSP 8 Score: 51.6026 bits (122), Expect = 1.518e-8
Identity = 27/83 (32.53%), Postives = 45/83 (54.22%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +H+A   G    A++LLD   N +  A   + P+H A +     +++LLL    + E  T+SG  P+H ++  G+L I+ ILL
Sbjct:   76 LHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILL 158          

HSP 9 Score: 51.2174 bits (121), Expect = 2.306e-8
Identity = 28/90 (31.11%), Postives = 43/90 (47.78%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A       VA LLLD   +    AK  + P+H A++    EI   LL+        T+ G  P+H  +  GH ++  +LL R
Sbjct:  302 NGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLAR 391          

HSP 10 Score: 48.1358 bits (113), Expect = 2.645e-7
Identity = 23/93 (24.73%), Postives = 49/93 (52.69%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            + + + G  P+H++   G +++  +LL    + +    R    +H A+R G +E+++ LL      + + +    P+H A+  G LEI+++LL
Sbjct:  132 EAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLL 224          

HSP 11 Score: 46.9802 bits (110), Expect = 5.231e-7
Identity = 25/91 (27.47%), Postives = 46/91 (50.55%), Query Frame = 1
Query:  223 IDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            I   G  P+H+  +EG  DVA LLL    +++   K    P+H A++     + ++L+    +    TK G+ P+  A   G+++++  LL
Sbjct:  365 ITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLL 455          

HSP 12 Score: 44.2838 bits (103), Expect = 4.513e-6
Identity = 24/88 (27.27%), Postives = 44/88 (50.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LLL    +I+   +    P+H ++  G+  I+ +LL    +       G   +H A+  G  E+++ LL
Sbjct:  104 NGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLL 191          

HSP 13 Score: 43.8986 bits (102), Expect = 5.989e-6
Identity = 21/70 (30.00%), Postives = 37/70 (52.86%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRP 444
            G+ P+ +AC  G + +   LL +  +++ + K  + P+H A++ GH+ I+ LLL          K G RP
Sbjct:  435 GYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLL----------KCGARP 494          
BLAST of SMED30027856 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002048.1 (pep scaffold:Pmarinus_7.0:GL477431:30859:73495:1 gene:ENSPMAG00000001850.1 transcript:ENSPMAT00000002048.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 68.1662 bits (165), Expect = 2.714e-14
Identity = 36/92 (39.13%), Postives = 56/92 (60.87%), Query Frame = 1
Query:  226 DDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKR-KWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            D  G  P+ +A   G V+V  LLLDN  +I+ +++R K  P+  A  GG  E+++LL+ R  N E    S + P+  A+SGG++ I+KILL+
Sbjct:  963 DKKGFTPLILAATAGHVNVVELLLDNGADIEAQSERTKDTPLSLACSGGRQEVVELLIARGANKEHRNVSDYTPLSLAASGGYVNIIKILLN 1054          

HSP 2 Score: 67.0106 bits (162), Expect = 6.886e-14
Identity = 30/86 (34.88%), Postives = 54/86 (62.79%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+  A   GFVD+ RLLL +  +++ ++      + YA  GG+ +++++LL+   N E   +SG  P+  A+SGGH+E+ ++LL+R
Sbjct:  170 PLMAAASGGFVDIVRLLLAHGADVNAQSSTGNTALTYACAGGYVDVVRVLLEAGANLEDHNESGHTPLMEAASGGHVEVARLLLER 255          

HSP 3 Score: 63.1586 bits (152), Expect = 1.570e-12
Identity = 39/117 (33.33%), Postives = 62/117 (52.99%), Query Frame = 1
Query:  145 CFKEQLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            C+K     G+ E VR  L+     +   D+ H  +  AC +G V+VARLLL++   ++  A     P+  A+ GGH E+  LL++R  N E     G+ P+  A+  GH E++ +LL
Sbjct:  275 CYK-----GHLEMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLESGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLL 386          

HSP 4 Score: 51.2174 bits (121), Expect = 2.161e-8
Identity = 28/89 (31.46%), Postives = 47/89 (52.81%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKS-GWRPVHWASSGGHLEILKILLD 498
            G+  +  AC  G+VDV R+LL+   N++   +    P+  A+ GGH E+ +LLL+R       +       +  A   GHLE+++ LL+
Sbjct:  200 GNTALTYACAGGYVDVVRVLLEAGANLEDHNESGHTPLMEAASGGHVEVARLLLERGAGINTHSNEFKESALTLACYKGHLEMVRFLLE 288          

HSP 5 Score: 50.0618 bits (118), Expect = 4.986e-8
Identity = 27/87 (31.03%), Postives = 48/87 (55.17%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAK-RKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+ +A   G     +LLLD   +I+ + +  +   +  A   G  E++ LL++R  N E   K+G  P+  A+SGG+ E+ ++LL+R
Sbjct: 1071 PLMLAAMNGHTAAVKLLLDMGSDINAQIETNRNTALTLACFQGRHEVVSLLVERKANVEHRAKTGLTPLMEAASGGYAEVGRVLLER 1157          

HSP 6 Score: 49.6766 bits (117), Expect = 6.756e-8
Identity = 25/84 (29.76%), Postives = 48/84 (57.14%), Query Frame = 1
Query:  253 IACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECET-KSGWRPVHWASSGGHLEILKILLDR 501
            +AC  G  D+  +L+    NI+   K+ + P+  A+  GH  +++LLLD   + E ++ ++   P+  A SGG  E++++L+ R
Sbjct:  939 LACAGGHEDLVTVLIARGANIEHRDKKGFTPLILAATAGHVNVVELLLDNGADIEAQSERTKDTPLSLACSGGRQEVVELLIAR 1022          

HSP 7 Score: 48.1358 bits (113), Expect = 2.170e-7
Identity = 35/148 (23.65%), Postives = 69/148 (46.62%), Query Frame = 1
Query:  163 MNGNAEGVRENLK-GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFE-----------------------CE------------TKSGWRPVHWASSGGHLEILKILLD 498
            MNG+   V+  L  G  ++  I+ + +  + +AC +G  +V  LL++   N++  AK    P+  A+ GG++E+ ++LL+R  +                         CE             K G  P+  A++GGHL+++++L++
Sbjct: 1077 MNGHTAAVKLLLDMGSDINAQIETNRNTALTLACFQGRHEVVSLLVERKANVEHRAKTGLTPLMEAASGGYAEVGRVLLERGADVNAAPVPSSRDTALTIAADKGHYKFCELVISRGAHIDVRNKKGNTPLWLAANGGHLDVVQLLVN 1224          

HSP 8 Score: 47.3654 bits (111), Expect = 4.095e-7
Identity = 27/87 (31.03%), Postives = 43/87 (49.43%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKS--GWRPVHWASSGGHLEILKILLD 498
            P+ +AC  G  +V  LL+    N +      + P+  A+ GG+  I+K+LL+        T S  G  P+  A+  GH   +K+LLD
Sbjct: 1003 PLSLACSGGRQEVVELLIARGANKEHRNVSDYTPLSLAASGGYVNIIKILLNAGAEINSRTGSKLGISPLMLAAMNGHTAAVKLLLD 1089          

HSP 9 Score: 46.2098 bits (108), Expect = 1.106e-6
Identity = 28/92 (30.43%), Postives = 48/92 (52.17%), Query Frame = 1
Query:  226 DDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKR-KWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            ++ GH P+  A   G V+VARLLL+    I+  +   K   +  A   GH E+++ LL+   + E +T      +  A   GH+E+ ++LL+
Sbjct:  230 NESGHTPLMEAASGGHVEVARLLLERGAGINTHSNEFKESALTLACYKGHLEMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLE 321          

HSP 10 Score: 45.8246 bits (107), Expect = 1.299e-6
Identity = 32/115 (27.83%), Postives = 55/115 (47.83%), Query Frame = 1
Query:  154 EQLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWR-PVHWASSGGHLEILKILL 495
            E  M+G+ E  R  L+      +  D    P+ +A   G V++A LL++   N++      + P+  A+R GH E++ LLL +  N   +T+      +  A  GG LE+   L+
Sbjct:  306 EACMDGHVEVARLLLESGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQTEETQETALTLACCGGFLEVADFLI 420          

HSP 11 Score: 44.2838 bits (103), Expect = 4.434e-6
Identity = 33/127 (25.98%), Postives = 57/127 (44.88%), Query Frame = 1
Query:  118 GNIEMGEFECFKEQLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPV-HWASSGGHLEILKILL 495
             ++E+G      E    G+ E V+  L            G   +  AC  G  DVA LLL    +++ E++    P+  A+R GH   ++ L+ +  +    T +    V   A +GGHL ++++LL
Sbjct:  424 ADLELGCSTPLMEAAQEGHLELVKYLLAAGANVHATTATGDTALTYACENGHTDVADLLLQAGADLEHESEGGRTPLMKAARAGHVCTVQFLISKGADVNRATANNDHTVLSLACAGGHLSVVELLL 550          

HSP 12 Score: 44.2838 bits (103), Expect = 4.562e-6
Identity = 22/86 (25.58%), Postives = 43/86 (50.00%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+  A +EG +++ + LL    N+          + YA   GH+++  LLL    + E E++ G  P+  A+  GH+  ++ L+ +
Sbjct:  433 PLMEAAQEGHLELVKYLLAAGANVHATTATGDTALTYACENGHTDVADLLLQAGADLEHESEGGRTPLMKAARAGHVCTVQFLISK 518          

HSP 13 Score: 43.5134 bits (101), Expect = 8.743e-6
Identity = 22/83 (26.51%), Postives = 42/83 (50.60%), Query Frame = 1
Query:  253 IACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +AC +G +++ R LL+   + + +       +  A   GH E+ +LLL+          S   P+  A+ GGH+E+  +L++R
Sbjct:  273 LACYKGHLEMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLESGAQVNMPADSFESPLTLAACGGHVELAALLIER 355          
BLAST of SMED30027856 vs. Ensembl Yeast
Match: GDE1 (Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes [Source:SGD;Acc:S000006031])

HSP 1 Score: 59.3066 bits (142), Expect = 2.319e-11
Identity = 30/80 (37.50%), Postives = 45/80 (56.25%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKR-KWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEI 480
            P+++ACR  F + A  LL N  +++   K   W  I  A+  G ++I+KLL+  N NF+ E + GW P+  A   GHL I
Sbjct:  542 PLYLACRLNFFEAAVCLLYNGADLEIREKLFGWTAIFVAAAEGFTDIVKLLIANNANFDIEDEGGWTPMEHAVLRGHLHI 621          

HSP 2 Score: 45.0542 bits (105), Expect = 1.595e-6
Identity = 21/57 (36.84%), Postives = 31/57 (54.39%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRN 405
            G   I +A  EGF D+ +LL+ NN N D E +  W P+ +A   GH  I  ++  R+
Sbjct:  573 GWTAIFVAAAEGFTDIVKLLIANNANFDIEDEGGWTPMEHAVLRGHLHIADMVQIRD 629          
BLAST of SMED30027856 vs. Ensembl Yeast
Match: PHO81 (Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress [Source:SGD;Acc:S000003465])

HSP 1 Score: 51.6026 bits (122), Expect = 9.782e-9
Identity = 23/56 (41.07%), Postives = 32/56 (57.14%), Query Frame = 1
Query:  334 KWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            KW PI YA R GHSE++  LL  N   + E  +G  P+ +A    H+++L  LL R
Sbjct:  592 KWTPIFYAVRSGHSEVITELLKHNARLDIEDDNGHSPLFYALWESHVDVLNALLQR 647          
BLAST of SMED30027856 vs. Ensembl Yeast
Match: NAS6 (Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6 [Source:SGD;Acc:S000003464])

HSP 1 Score: 45.0542 bits (105), Expect = 9.858e-7
Identity = 30/93 (32.26%), Postives = 47/93 (50.54%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWR-PIHYASRGGHSEILKLLLDR--NCNFE-CETKSGWRPVHWASSGGHLEILKILLDR 501
             ++P+H AC E      + LL +  ++  +  +  R P+H++      EI   LL +  N N +     SGW P H A S G+LE++K L DR
Sbjct:    2 SNYPLHQACMENEFFKVQELLHSKPSLLLQKDQDGRIPLHWSVSFQAHEITSFLLSKMENVNLDDYPDDSGWTPFHIACSVGNLEVVKSLYDR 94          
BLAST of SMED30027856 vs. Ensembl Nematostella
Match: EDO45485 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSW0])

HSP 1 Score: 56.9954 bits (136), Expect = 9.105e-12
Identity = 26/55 (47.27%), Postives = 38/55 (69.09%), Query Frame = 1
Query:  337 WRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            + P+H A   GH EI+KLLLD   + E +TK+G++P+H A+  GH  I++ILL R
Sbjct:    2 FTPLHIAVVYGHWEIVKLLLDEGADVEAQTKNGYQPLHLAAQYGHKIIIEILLQR 56          

HSP 2 Score: 51.6026 bits (122), Expect = 1.311e-9
Identity = 21/64 (32.81%), Postives = 38/64 (59.38%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECET 426
            G  P+HIA   G  ++ +LLLD   +++ + K  ++P+H A++ GH  I+++LL R    +  T
Sbjct:    1 GFTPLHIAVVYGHWEIVKLLLDEGADVEAQTKNGYQPLHLAAQYGHKIIIEILLQRGAPPDALT 64          
BLAST of SMED30027856 vs. Ensembl Nematostella
Match: EDO46099 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR35])

HSP 1 Score: 58.9214 bits (141), Expect = 1.056e-11
Identity = 30/89 (33.71%), Postives = 50/89 (56.18%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWR---PIHYASRGGHSE-ILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            PIH A   G  +  R+LL    + + +  +  R   P+HYA+R GH +  +++L++R  + E   + GWR +H AS  G +++L  LLD
Sbjct:   33 PIHEAAMNGHTECVRMLLRYGSHANAKTGKTIRNMTPLHYAARMGHLDCAVRMLMERGIDLEAVYRDGWRLIHVASDNGRVKVLVTLLD 121          

HSP 2 Score: 52.373 bits (124), Expect = 3.617e-9
Identity = 28/89 (31.46%), Postives = 49/89 (55.06%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWR---PVHWASSGGHLE-ILKILLDR 501
            +H A R G      LLL +   ++   +  WRPIH A+  GH+E +++LL    +   +T    R   P+H+A+  GHL+  +++L++R
Sbjct:    1 LHYAARYGNEKCIELLLKSGAKLNVPQRYGWRPIHEAAMNGHTECVRMLLRYGSHANAKTGKTIRNMTPLHYAARMGHLDCAVRMLMER 89          

HSP 3 Score: 47.7506 bits (112), Expect = 1.784e-7
Identity = 36/119 (30.25%), Postives = 57/119 (47.90%), Query Frame = 1
Query:  154 EQLMNGNAEGVRENLK-----GEFVDKVIDDDGHFPIHIACREGFVDVA-RLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECET-KSGWRPVHWASSGGHLEILKI 489
            E  MNG+ E VR  L+          K I +    P+H A R G +D A R+L++   +++   +  WR IH AS  G  ++L  LLD   + E +T +     VH A+  G + +  +
Sbjct:   36 EAAMNGHTECVRMLLRYGSHANAKTGKTIRN--MTPLHYAARMGHLDCAVRMLMERGIDLEAVYRDGWRLIHVASDNGRVKVLVTLLDAGVDIESKTYERRLTAVHVAARQGQVTMTTL 152          
BLAST of SMED30027856 vs. Ensembl Nematostella
Match: EDO35987 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJZ9])

HSP 1 Score: 57.3806 bits (137), Expect = 1.894e-11
Identity = 30/91 (32.97%), Postives = 47/91 (51.65%), Query Frame = 1
Query:  241 FPIHIACREGFVDVARLLLDNNCNI----DCEAKRKW--RPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
              +H A REGF D+ARLL+    N+    D +A   +    +HYA++ GH E++KLL+ R  +       G  P+  A+     E + +LL
Sbjct:    1 MALHYAVREGFADIARLLIKKGSNLEATTDMDAHDMFGRTALHYAAQQGHMEMIKLLVTRGADIHTRDSEGQTPLFQATIFDQKEAVNVLL 91          

HSP 2 Score: 44.669 bits (104), Expect = 9.849e-7
Identity = 20/59 (33.90%), Postives = 34/59 (57.63%), Query Frame = 1
Query:  343 PIHYASRGGHSEILKLLLDRNCNFECETKS------GWRPVHWASSGGHLEILKILLDR 501
             +HYA R G ++I +LL+ +  N E  T        G   +H+A+  GH+E++K+L+ R
Sbjct:    2 ALHYAVREGFADIARLLIKKGSNLEATTDMDAHDMFGRTALHYAAQQGHMEMIKLLVTR 60          
BLAST of SMED30027856 vs. Ensembl Nematostella
Match: EDO38702 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC57])

HSP 1 Score: 59.6918 bits (143), Expect = 3.455e-11
Identity = 31/85 (36.47%), Postives = 44/85 (51.76%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            P+  A   G V+VA +LLD +  +D   K K  P+H ASR GH E++KLLL R  N      +G   +  A    H E+   ++D
Sbjct:  353 PLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSRKANITLTDSAGRNCLDIAIESSHKEVAVAIID 437          

HSP 2 Score: 58.151 bits (139), Expect = 1.582e-10
Identity = 32/94 (34.04%), Postives = 49/94 (52.13%), Query Frame = 1
Query:  226 DDDG--HFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            D+DG  + P+H+A   G   V + LL     ID      W P+  A+  G  E+  +LLD +   +   K+   P+H AS  GH+E++K+LL R
Sbjct:  312 DEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSR 405          
BLAST of SMED30027856 vs. Ensembl Nematostella
Match: EDO34034 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQH2])

HSP 1 Score: 57.3806 bits (137), Expect = 4.607e-11
Identity = 35/92 (38.04%), Postives = 44/92 (47.83%), Query Frame = 1
Query:  226 DDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHS-EILKLLLDRNCNFECETKSGWRPVHWASSG-GHLEILKILL 495
            D DG+  +H A  EG VDVA++LL    N+       W P+H A R G   E   LLL    N    T     P+H A+ G G    LK+LL
Sbjct:   28 DADGYSALHRASYEGHVDVAKVLLTYGANVMATTHDGWHPLHSACRWGKGVEAASLLLQNGANINALTNGKQTPLHLAALGIGGTATLKLLL 119          
BLAST of SMED30027856 vs. Ensembl Medaka
Match: ENSORLT00000039903.1 (pep primary_assembly:ASM223467v1:15:18469388:18484014:-1 gene:ENSORLG00000030042.1 transcript:ENSORLT00000039903.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 81.2629 bits (199), Expect = 3.100e-18
Identity = 37/90 (41.11%), Postives = 56/90 (62.22%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
             G  P+HIA   G ++VA LLL+    +D  A+    P+H A++ G+S ++KLLLDR    E +TK G  P+H  +  GH ++++ILLDR
Sbjct:  210 SGFTPLHIAAHYGNINVATLLLNRAAAVDFMARNDITPLHVAAKRGNSNMVKLLLDRGARIEAKTKDGLTPLHCGARSGHEQVVEILLDR 299          

HSP 2 Score: 73.1738 bits (178), Expect = 2.505e-15
Identity = 30/85 (35.29%), Postives = 52/85 (61.18%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            P+H+A + G  ++ +LLLD    I+ + K    P+H  +R GH +++++LLDR      +TK+G  P+H A+ G HL  +++LL 
Sbjct:  247 PLHVAAKRGNSNMVKLLLDRGARIEAKTKDGLTPLHCGARSGHEQVVEILLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQ 331          

HSP 3 Score: 64.6994 bits (156), Expect = 1.891e-12
Identity = 31/110 (28.18%), Postives = 60/110 (54.55%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            GN E V + LK      + + +G   +H+A +EG V+V   LL    ++D   K+    +H +S  G +E++  L+    N   ++++G+ P++ A+   HLE+++ LL+
Sbjct:   28 GNLEKVLDYLKSGVEINICNQNGLNALHLASKEGHVEVVAELLKLGASVDAATKKGNTALHISSLAGQAEVVTELVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLE 137          

HSP 4 Score: 62.3882 bits (150), Expect = 1.365e-11
Identity = 34/108 (31.48%), Postives = 59/108 (54.63%), Query Frame = 1
Query:  178 EGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            E VR  L+      +  +DG  P+ +A ++G   V  LLL+N    D + K +   +H A+R   ++   LLL  + N + E+KSG+ P+H A+  G++ +  +LL+R
Sbjct:  130 EVVRFLLENSASQSIATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNR 233          

HSP 5 Score: 60.8474 bits (146), Expect = 4.190e-11
Identity = 30/85 (35.29%), Postives = 49/85 (57.65%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R+     A LLL N+ N D E+K  + P+H A+  G+  +  LLL+R    +   ++   P+H A+  G+  ++K+LLDR
Sbjct:  182 LHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFMARNDITPLHVAAKRGNSNMVKLLLDR 266          

HSP 6 Score: 56.6102 bits (135), Expect = 1.445e-9
Identity = 26/92 (28.26%), Postives = 49/92 (53.26%), Query Frame = 1
Query:  223 IDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            + +D    +H+A   G   VA+L++D   N + +A   + P+H A +     +++LLL    + +  T+SG  P+H A+  GH  I+  L++
Sbjct:  339 VTNDYLTALHVAAHCGHYKVAKLIVDKKANPNAKALNGFTPLHIACKKNRVRVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVSALIN 430          

HSP 7 Score: 53.9138 bits (128), Expect = 1.147e-8
Identity = 27/90 (30.00%), Postives = 45/90 (50.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            DG  P+H   R G   V  +LLD    I  + K    P+H A++G H   ++LLL  +   +  T      +H A+  GH ++ K+++D+
Sbjct:  276 DGLTPLHCGARSGHEQVVEILLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKLIVDK 365          

HSP 8 Score: 48.9062 bits (115), Expect = 5.908e-7
Identity = 22/88 (25.00%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +   ++  +LLL ++  +D         +H A+  GH ++ KL++D+  N   +  +G+ P+H A     + ++++LL
Sbjct:  309 NGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKLIVDKKANPNAKALNGFTPLHIACKKNRVRVMELLL 396          
BLAST of SMED30027856 vs. Ensembl Medaka
Match: ank1b (ankyrin-1 [Source:NCBI gene;Acc:101157380])

HSP 1 Score: 81.6481 bits (200), Expect = 3.440e-18
Identity = 37/85 (43.53%), Postives = 53/85 (62.35%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            P+H+A R G  +VA+ LL N   +D  AK    P+H A+R GH E++KLLLD   N +  T +G  P+H A+  GH+  ++ILLD
Sbjct:  438 PLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLD 522          

HSP 2 Score: 77.7962 bits (190), Expect = 6.068e-17
Identity = 35/88 (39.77%), Postives = 55/88 (62.50%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIA R G V + RLLLD    ID + K +  P+H A+R GH  I+++LL+     + +TK+G  P+H A+ G H++ ++ LL
Sbjct:  236 NGITPLHIASRRGNVMMVRLLLDRGAQIDAQTKDELTPLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLL 323          

HSP 3 Score: 71.2478 bits (173), Expect = 1.288e-14
Identity = 35/93 (37.63%), Postives = 55/93 (59.14%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            V+   G  P+HIA     + VA+LLL+   N++   K    P+H ASR G+  +++LLLDR    + +TK    P+H A+  GH+ I++ILL+
Sbjct:  199 VLSKTGFTPLHIAAHYENMSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVMMVRLLLDRGAQIDAQTKDELTPLHCAARNGHVRIIEILLE 291          

HSP 4 Score: 67.0106 bits (162), Expect = 4.011e-13
Identity = 32/89 (35.96%), Postives = 52/89 (58.43%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            GH P+HIA REG +   R+LLD         K+ + P+H AS+ G  ++ +LLL+R  N     K+G  P+H A    +L+++K+L+ +
Sbjct:  501 GHTPLHIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSK 589          

HSP 5 Score: 65.4698 bits (158), Expect = 1.357e-12
Identity = 35/97 (36.08%), Postives = 53/97 (54.64%), Query Frame = 1
Query:  211 VDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            VD    DD   P+H A R G  ++ +LLLD+  N D        P+H A+R GH   +++LLD        TK G+ P+H AS  G +++ ++LL+R
Sbjct:  461 VDARAKDD-QTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLLER 556          

HSP 6 Score: 63.1586 bits (152), Expect = 8.628e-12
Identity = 27/90 (30.00%), Postives = 53/90 (58.89%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+ P+HIA ++  ++VA +LL N  + +CE+ +   P+H AS+ G  +++ +L+ +  N     K+G  P+H  +  GH+ I   L+ +
Sbjct:  599 NGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLVKQ 688          

HSP 7 Score: 61.6178 bits (148), Expect = 2.697e-11
Identity = 41/123 (33.33%), Postives = 60/123 (48.78%), Query Frame = 1
Query:  142 ECFKEQLMNGNAEGVRENLKGEFVDKVIDD---DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +C + QL+  NAE              IDD   D   P+H+A   G   +A++LLD     +  A   + P+H A +  H   + LLL  + + E  T+SG  P+H A+  GHL I+K LL R
Sbjct:  317 DCVR-QLLQYNAE--------------IDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLLQR 424          

HSP 8 Score: 60.077 bits (144), Expect = 1.001e-10
Identity = 28/94 (29.79%), Postives = 49/94 (52.13%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            + + + G  P+H+A   G +++ + LL    + +    +   P+H ASR GH E+ + LL      +   K    P+H A+  GH E++K+LLD
Sbjct:  396 EAVTESGLTPLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLD 489          

HSP 9 Score: 59.3066 bits (142), Expect = 1.918e-10
Identity = 37/112 (33.04%), Postives = 57/112 (50.89%), Query Frame = 1
Query:  169 GNAEGVRENL-KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            GN   VR  L +G  +D    D+   P+H A R G V +  +LL++   I  + K    PIH A++G H + ++ LL  N   +  T     P+H A+  GH  + K+LLD+
Sbjct:  248 GNVMMVRLLLDRGAQIDAQTKDE-LTPLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDK 358          

HSP 10 Score: 58.9214 bits (141), Expect = 2.758e-10
Identity = 25/90 (27.78%), Postives = 52/90 (57.78%), Query Frame = 1
Query:  226 DDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            + +G  P+H+  +EG V +A  L+    ++   ++  + P+H A   G+ +++K LL +  +   +T+ G+ P+H A+  GH +I+ +LL
Sbjct:  663 NKNGLTPLHLVAQEGHVGIADTLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNAKTRMGYTPLHQAAQQGHTDIVTLLL 752          

HSP 11 Score: 58.151 bits (139), Expect = 4.004e-10
Identity = 27/87 (31.03%), Postives = 48/87 (55.17%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G  P+H+A + G VDVA LLL+   N +   K    P+H A    + +++KLL+ +  +     ++G+ P+H A+    +E+  +LL
Sbjct:  534 GFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSAHSTARNGYTPLHIAAKQNQIEVASVLL 620          

HSP 12 Score: 57.3806 bits (137), Expect = 7.889e-10
Identity = 30/85 (35.29%), Postives = 49/85 (57.65%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R      A +LL N+ N D  +K  + P+H A+   +  + +LLL+R  N     K+G  P+H AS  G++ ++++LLDR
Sbjct:  175 LHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENMSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVMMVRLLLDR 259          

HSP 13 Score: 54.299 bits (129), Expect = 9.518e-9
Identity = 28/88 (31.82%), Postives = 46/88 (52.27%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  +    LLL ++ +++   +    P+H A+  GH  I+K LL R  +          P+H AS  GH E+ + LL
Sbjct:  368 NGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLL 455          

HSP 14 Score: 53.9138 bits (128), Expect = 1.341e-8
Identity = 28/88 (31.82%), Postives = 43/88 (48.86%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  PIH+A +   +D  R LL  N  ID        P+H A+  GH  + K+LLD+         +G+ P+H A    H+  + +LL
Sbjct:  302 NGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLL 389          

HSP 15 Score: 53.9138 bits (128), Expect = 1.380e-8
Identity = 23/86 (26.74%), Postives = 48/86 (55.81%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            G+ P+H+AC  G + + + LL    +++ + +  + P+H A++ GH++I+ LLL         T +G  P+  A   G++ ++ +L
Sbjct:  699 GYTPLHVACHYGNIKMVKFLLQQQAHVNAKTRMGYTPLHQAAQQGHTDIVTLLLKHGALPNEITSNGTSPLGIAKRLGYISVIDVL 784          

HSP 16 Score: 52.7582 bits (125), Expect = 2.901e-8
Identity = 26/111 (23.42%), Postives = 58/111 (52.25%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +GN +   +++K      + + +G   +H+A +EG V +   LL +   ++   K+    +H A+  G  +++  L++   N   ++  G+ P++ A+   HLE++K LL+
Sbjct:   20 SGNLDKALDHIKNGININIANQNGLNGLHLASKEGHVKMVLELLHSGIELEATTKKGNTALHIAALAGQEKVVAELVNYGANVNAQSHKGFSPLYMAAQENHLEVVKFLLE 130          

HSP 17 Score: 52.373 bits (124), Expect = 3.897e-8
Identity = 24/90 (26.67%), Postives = 47/90 (52.22%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G  P+H+A     +DV +LL+    +    A+  + P+H A++    E+  +LL    +  CE+  G  P+H AS  G  +++ +L+ +
Sbjct:  566 NGLTPLHVAVHHNNLDVVKLLVSKGGSAHSTARNGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLISK 655          

HSP 18 Score: 51.6026 bits (122), Expect = 6.831e-8
Identity = 24/89 (26.97%), Postives = 48/89 (53.93%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  P+H+A +EG  D+  +L+    N++   K    P+H  ++ GH  I   L+ +  +    ++ G+ P+H A   G+++++K LL +
Sbjct:  633 GITPLHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQ 721          
BLAST of SMED30027856 vs. Ensembl Medaka
Match: ENSORLT00000040233.1 (pep primary_assembly:ASM223467v1:19:16412534:16434057:1 gene:ENSORLG00000021910.1 transcript:ENSORLT00000040233.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 77.7962 bits (190), Expect = 4.600e-17
Identity = 34/90 (37.78%), Postives = 57/90 (63.33%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
             G  P+HIA   G ++VA LLL+    +D +A+    P+H AS+ G+S +++LLL+R    +  TK G  P+H A+  GH +++++LL+R
Sbjct:  210 SGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVRLLLERGSKIDARTKDGLTPLHCAARSGHEQVVEMLLNR 299          

HSP 2 Score: 74.3294 bits (181), Expect = 8.810e-16
Identity = 31/84 (36.90%), Postives = 52/84 (61.90%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            P+H+A + G  ++ RLLL+    ID   K    P+H A+R GH +++++LL+R      +TK+G  P+H A+ G HL  +++LL
Sbjct:  247 PLHVASKRGNSNMVRLLLERGSKIDARTKDGLTPLHCAARSGHEQVVEMLLNRGAPILSKTKNGLSPLHMATQGDHLNCVQLLL 330          

HSP 3 Score: 66.6254 bits (161), Expect = 4.549e-13
Identity = 30/110 (27.27%), Postives = 61/110 (55.45%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            GN E   + L+      + + +G   +H+A +EG V+V   L+    N+D   K+    +H AS  G +E++K L+    N   ++++G+ P++ A+   HL+++++LL+
Sbjct:   28 GNLEKALDYLQNGVDINICNQNGLNALHLASKEGHVEVVAELIKQGANVDAATKKGNTALHIASLAGQTEVVKELVSNGANVNAQSQNGFTPLYMAAQENHLDVVQLLLE 137          

HSP 4 Score: 60.4622 bits (145), Expect = 5.124e-11
Identity = 29/85 (34.12%), Postives = 50/85 (58.82%), Query Frame = 1
Query:  247 IHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +HIA R+     A LLL N+ N D E+K  + P+H A+  G+  +  LLL+R    + + ++   P+H AS  G+  ++++LL+R
Sbjct:  182 LHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVRLLLER 266          

HSP 5 Score: 60.4622 bits (145), Expect = 6.387e-11
Identity = 41/144 (28.47%), Postives = 70/144 (48.61%), Query Frame = 1
Query:  133 GEFECFKEQLMNG---NAE----------GVREN--------LKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G+ E  KE + NG   NA+            +EN        L+      +  +DG  P+ +A ++G   V  LLL+N    D + K +   +H A+R   ++   LLL  + N + E+KSG+ P+H A+  G++ +  +LL+R
Sbjct:   94 GQTEVVKELVSNGANVNAQSQNGFTPLYMAAQENHLDVVQLLLENGSSQSIATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNR 233          

HSP 6 Score: 58.5362 bits (140), Expect = 2.845e-10
Identity = 31/111 (27.93%), Postives = 62/111 (55.86%), Query Frame = 1
Query:  169 GNAEGVRENLK-GEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            G+ E V E +K G  VD      G+  +HIA   G  +V + L+ N  N++ +++  + P++ A++  H ++++LLL+   +    T+ G+ P+  A   GH +++ +LL+
Sbjct:   61 GHVEVVAELIKQGANVDAATKK-GNTALHIASLAGQTEVVKELVSNGANVNAQSQNGFTPLYMAAQENHLDVVQLLLENGSSQSIATEDGFTPLAVALQQGHDQVVSLLLE 170          

HSP 7 Score: 54.6842 bits (130), Expect = 5.667e-9
Identity = 34/112 (30.36%), Postives = 56/112 (50.00%), Query Frame = 1
Query:  169 GNAEGVRENL-KGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            GN+  VR  L +G  +D    D G  P+H A R G   V  +LL+    I  + K    P+H A++G H   ++LLL  +   +  T      +H A+  GH ++ K+++D+
Sbjct:  255 GNSNMVRLLLERGSKIDARTKD-GLTPLHCAARSGHEQVVEMLLNRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDK 365          

HSP 8 Score: 54.299 bits (129), Expect = 6.794e-9
Identity = 25/92 (27.17%), Postives = 50/92 (54.35%), Query Frame = 1
Query:  223 IDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            + +D    +H+A   G   VA++++D   N + +A   + P+H A +    ++++LLL    + +  T+SG  P+H A+  GH  I+  L++
Sbjct:  339 VTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVHQLIN 430          

HSP 9 Score: 47.3654 bits (111), Expect = 1.634e-6
Identity = 21/88 (23.86%), Postives = 49/88 (55.68%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+H+A +   ++  +LLL ++  +D         +H A+  GH ++ K+++D+  N   +  +G+ P+H A     ++++++LL
Sbjct:  309 NGLSPLHMATQGDHLNCVQLLLHHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLL 396          
BLAST of SMED30027856 vs. Ensembl Medaka
Match: ank1a (ankyrin-1 [Source:NCBI gene;Acc:101174837])

HSP 1 Score: 77.7962 bits (190), Expect = 7.569e-17
Identity = 35/85 (41.18%), Postives = 53/85 (62.35%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            P+H+A R G  +VA  LL N   +D +AK    P+H A+R GH E++KLLL+   N    T +G  P+H A+  GH++ +++LLD
Sbjct:  378 PLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLLD 462          

HSP 2 Score: 67.781 bits (164), Expect = 1.933e-13
Identity = 33/89 (37.08%), Postives = 51/89 (57.30%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            GH P+HIA REG V   RLLLD         K+ + P+H AS+ G  ++ +LLL+R  N     K+G  P+H A    +L+++ +L+ +
Sbjct:  441 GHSPLHIAAREGHVQTVRLLLDMEAQQTKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSK 529          

HSP 3 Score: 66.2402 bits (160), Expect = 8.078e-13
Identity = 43/123 (34.96%), Postives = 62/123 (50.41%), Query Frame = 1
Query:  142 ECFKEQLMNGNAEGVRENLKGEFVDKVIDD---DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +C K QL+  NAE              IDD   D   P+H+A   G   +A++LLD     +  A   + P+H A +  H  ++ LLL  + + E  T+SG  P+H AS  GHL I+KILL +
Sbjct:  257 DCVK-QLLQYNAE--------------IDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNSRALNGFTPLHIACKKNHLRVMDLLLKHSASIEAVTESGLTPLHVASFMGHLNIVKILLQK 364          

HSP 4 Score: 64.6994 bits (156), Expect = 2.783e-12
Identity = 34/97 (35.05%), Postives = 54/97 (55.67%), Query Frame = 1
Query:  211 VDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            VD    DD   P+H A R G  ++ +LLL++  N +        P+H A+R GH + ++LLLD        TK G+ P+H AS  G +++ ++LL+R
Sbjct:  401 VDAKAKDD-QTPLHCAARMGHKELVKLLLEHKANPNSTTTAGHSPLHIAAREGHVQTVRLLLDMEAQQTKMTKKGFTPLHVASKYGKVDVAELLLER 496          

HSP 5 Score: 63.5438 bits (153), Expect = 6.347e-12
Identity = 32/87 (36.78%), Postives = 51/87 (58.62%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G  P+HIA     ++VA+LLL+   N       +  P+H A+R GH  I+++LLD     + +TK+G  P+H A+ G H++ +K LL
Sbjct:  183 GFTPLHIAAHYENLNVAQLLLNRGAN------DELTPLHCAARNGHFRIIEILLDNGAPIQAKTKNGLSPIHMAAQGDHMDCVKQLL 263          

HSP 6 Score: 61.6178 bits (148), Expect = 2.696e-11
Identity = 32/91 (35.16%), Postives = 47/91 (51.65%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +D   P+H A R G   +  +LLDN   I  + K    PIH A++G H + +K LL  N   +  T     P+H A+  GH  + K+LLD+
Sbjct:  208 NDELTPLHCAARNGHFRIIEILLDNGAPIQAKTKNGLSPIHMAAQGDHMDCVKQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDK 298          

HSP 7 Score: 59.6918 bits (143), Expect = 1.166e-10
Identity = 30/88 (34.09%), Postives = 48/88 (54.55%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  P+HIAC++  + V  LLL ++ +I+   +    P+H AS  GH  I+K+LL +  +          P+H AS  GH E+ + LL
Sbjct:  308 NGFTPLHIACKKNHLRVMDLLLKHSASIEAVTESGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLL 395          

HSP 8 Score: 59.3066 bits (142), Expect = 2.011e-10
Identity = 29/90 (32.22%), Postives = 51/90 (56.67%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            +G+  +HIA ++  V+VA  LL +  + + E+ +   P+H AS+ G  +I+ LL+ +  N     KSG  P+H  +  GH+ I  IL+ +
Sbjct:  539 NGYTALHIAAKQNQVEVANSLLQHGASANAESLQGVTPLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQ 628          

HSP 9 Score: 58.151 bits (139), Expect = 4.753e-10
Identity = 27/94 (28.72%), Postives = 50/94 (53.19%), Query Frame = 1
Query:  217 KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            + + + G  P+H+A   G +++ ++LL    +      +   P+H ASR GH E+ + LL      + + K    P+H A+  GH E++K+LL+
Sbjct:  336 EAVTESGLTPLHVASFMGHLNIVKILLQKGASPSASNVKVETPLHMASRSGHFEVAEFLLQNAAPVDAKAKDDQTPLHCAARMGHKELVKLLLE 429          

HSP 10 Score: 55.8398 bits (133), Expect = 2.456e-9
Identity = 26/89 (29.21%), Postives = 49/89 (55.06%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  P+H+A +EG  D+  LL+    N++   K    P+H  ++ GH  I  +L+ +  +    T+ G+ P+H A   G+++++K LL +
Sbjct:  573 GVTPLHLASQEGRPDIVSLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKQGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQ 661          

HSP 11 Score: 55.4546 bits (132), Expect = 3.737e-9
Identity = 28/111 (25.23%), Postives = 58/111 (52.25%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            +GN +   E++K        + +G   +H+A +EG V +   LL N   ++   K+    +H A+  G  +++  L++   N   +++ G+ P++ A+   HLE++K LL+
Sbjct:   17 SGNLDKALEHIKNGIDINTANQNGLNALHLASKEGHVKMVLELLHNGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGTNVNAQSQKGFTPLYMAAQENHLEVVKFLLE 127          

HSP 12 Score: 54.299 bits (129), Expect = 8.169e-9
Identity = 28/88 (31.82%), Postives = 44/88 (50.00%), Query Frame = 1
Query:  232 DGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            +G  PIH+A +   +D  + LL  N  ID        P+H A+  GH  + K+LLD+         +G+ P+H A    HL ++ +LL
Sbjct:  242 NGLSPIHMAAQGDHMDCVKQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKPNSRALNGFTPLHIACKKNHLRVMDLLL 329          

HSP 13 Score: 52.373 bits (124), Expect = 4.579e-8
Identity = 30/109 (27.52%), Postives = 53/109 (48.62%), Query Frame = 1
Query:  169 GNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            G+ + VR  L  E     +   G  P+H+A + G VDVA LLL+   N +   K    P+H A    + +++ LL+ +  +     ++G+  +H A+    +E+   LL
Sbjct:  452 GHVQTVRLLLDMEAQQTKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVNLLVSKGGSPHSAARNGYTALHIAAKQNQVEVANSLL 560          
BLAST of SMED30027856 vs. Ensembl Medaka
Match: ankrd52a (serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C [Source:NCBI gene;Acc:101155432])

HSP 1 Score: 76.2554 bits (186), Expect = 2.667e-16
Identity = 36/92 (39.13%), Postives = 59/92 (64.13%), Query Frame = 1
Query:  220 VIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            + D  G   +H A + GF ++ +LLL+   N+    K++ +PIH A+  GH E++KLLL R+ +  C+ K G+ P+H A++ GH+EI+K LL
Sbjct:  135 IADRTGRTALHHAAQSGFQEMVKLLLNKGANLSAIDKKERQPIHCAAYLGHLEVVKLLLSRSNDKSCKDKLGYTPLHAAAASGHIEIVKYLL 226          

HSP 2 Score: 56.6102 bits (135), Expect = 1.510e-9
Identity = 28/86 (32.56%), Postives = 48/86 (55.81%), Query Frame = 1
Query:  244 PIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            P+H+A        A  LL +  N++   +     +H+A++ G  E++KLLL++  N     K   +P+H A+  GHLE++K+LL R
Sbjct:  110 PLHVASANRATRCAEALLSHLSNLNIADRTGRTALHHAAQSGFQEMVKLLLNKGANLSAIDKKERQPIHCAAYLGHLEVVKLLLSR 195          

HSP 3 Score: 52.373 bits (124), Expect = 4.805e-8
Identity = 36/113 (31.86%), Postives = 55/113 (48.67%), Query Frame = 1
Query:  166 NGNAEGVRENLK-GEFVD--KVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILL 495
            NG+++ +R  +  GE  D   V D  G  P+ +A   G  D  R LL+   + D + KR    +H A+  GH + +  LL    +  C    G  P+H+A+S G  EIL  L+
Sbjct:  656 NGHSDCLRMMIDYGEEGDLTNVADKFGQTPLMLAVLGGHTDCVRFLLERASSPDAQDKRGSTALHRAAVLGHDDCVTALLKHKASPLCRDIQGRTPLHYAASRGQTEILSSLM 768          

HSP 4 Score: 45.4394 bits (106), Expect = 9.834e-6
Identity = 27/83 (32.53%), Postives = 39/83 (46.99%), Query Frame = 1
Query:  223 IDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGH 471
            ID     PIH A   G ++V +LLL  + +  C+ K  + P+H A+  GH EI+K LL      +     G   +H A   G 
Sbjct:  169 IDKKERQPIHCAAYLGHLEVVKLLLSRSNDKSCKDKLGYTPLHAAAASGHIEIVKYLLRMGAEIDEPNTFGNTALHMACYMGQ 251          
BLAST of SMED30027856 vs. Planmine SMEST
Match: SMESG000010599.1 (SMESG000010599.1)

HSP 1 Score: 261.922 bits (668), Expect = 5.474e-85
Identity = 124/124 (100.00%), Postives = 124/124 (100.00%), Query Frame = 1
Query:  130 MGEFECFKEQLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            MGEFECFKEQLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR
Sbjct:    1 MGEFECFKEQLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 124          

HSP 2 Score: 123.25 bits (308), Expect = 5.862e-33
Identity = 58/90 (64.44%), Postives = 70/90 (77.78%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLD 498
            + G+ PIH A   G  ++ +LLLD+NCN +CEA +  RPIH+AS GGHSEILKLLLD NCN ECETKSG RP+HWAS  GHL I+K+LLD
Sbjct:  133 NTGNRPIHCAASGGHSEILKLLLDSNCNFECEANQGERPIHFASWGGHSEILKLLLDCNCNIECETKSGMRPIHWASREGHLNIIKVLLD 222          

HSP 3 Score: 105.916 bits (263), Expect = 7.080e-27
Identity = 51/91 (56.04%), Postives = 63/91 (69.23%), Query Frame = 1
Query:  229 DDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            + G  PIH A   G  ++ +LLLD NCNI+CE K   RPIH+ASR GH  I+K+LLD NCN EC TK  WRP+H+A   GH EILK+LL +
Sbjct:  166 NQGERPIHFASWGGHSEILKLLLDCNCNIECETKSGMRPIHWASREGHLNIIKVLLDCNCNIECMTKLKWRPIHYAIKEGHSEILKLLLRK 256          

HSP 4 Score: 87.4261 bits (215), Expect = 2.367e-20
Identity = 40/65 (61.54%), Postives = 50/65 (76.92%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETK 429
            G  PIH A REG +++ ++LLD NCNI+C  K KWRPIHYA + GHSEILKLLL +NC  +C+TK
Sbjct:  201 GMRPIHWASREGHLNIIKVLLDCNCNIECMTKLKWRPIHYAIKEGHSEILKLLLRKNCRIDCKTK 265          
BLAST of SMED30027856 vs. Planmine SMEST
Match: SMESG000010218.1 (SMESG000010218.1)

HSP 1 Score: 188.348 bits (477), Expect = 4.784e-58
Identity = 88/125 (70.40%), Postives = 105/125 (84.00%), Query Frame = 1
Query:  130 MGEFECFKEQLMNGNAEGVRENLKGEFVDKVI-DDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            MG+F+ FK+ LM GNAEGV  NL+GEFVDK++ D +G+F IHIACR+GFV VA+LLLD NCNI+CE+K   RPIHYASR GHSEI+KLLLDRNCN ECE KSG RP+HWAS  GH EI+K+LL+R
Sbjct:    1 MGDFQSFKKNLMKGNAEGVMNNLRGEFVDKIVCDYNGYFSIHIACRDGFVAVAKLLLDRNCNIECESKSGMRPIHYASRQGHSEIVKLLLDRNCNTECEDKSGKRPIHWASRNGHTEIVKLLLER 125          
BLAST of SMED30027856 vs. Planmine SMEST
Match: SMESG000010218.1 (SMESG000010218.1)

HSP 1 Score: 188.348 bits (477), Expect = 5.121e-58
Identity = 88/125 (70.40%), Postives = 105/125 (84.00%), Query Frame = 1
Query:  130 MGEFECFKEQLMNGNAEGVRENLKGEFVDKVI-DDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            MG+F+ FK+ LM GNAEGV  NL+GEFVDK++ D +G+F IHIACR+GFV VA+LLLD NCNI+CE+K   RPIHYASR GHSEI+KLLLDRNCN ECE KSG RP+HWAS  GH EI+K+LL+R
Sbjct:    1 MGDFQSFKKNLMKGNAEGVMNNLRGEFVDKIVCDYNGYFSIHIACRDGFVAVAKLLLDRNCNIECESKSGMRPIHYASRQGHSEIVKLLLDRNCNTECEDKSGKRPIHWASRNGHTEIVKLLLER 125          
BLAST of SMED30027856 vs. Planmine SMEST
Match: SMESG000010579.1 (SMESG000010579.1)

HSP 1 Score: 194.126 bits (492), Expect = 1.025e-57
Identity = 90/125 (72.00%), Postives = 104/125 (83.20%), Query Frame = 1
Query:  130 MGEFECFKEQLMNGNAEGVRENLKGEFVDKVIDDD-GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            MG+++ FK+ LM GNAEGVR NL+GEFVDK++DD  G FPIH+ACR GFV VA+LLLD NCNI+CE K  WRPIH ASR GHSEI+KLLLDRNCN ECE KSG RP+HWAS  GH EI+K+LLDR
Sbjct:    1 MGDYQSFKKHLMRGNAEGVRNNLRGEFVDKIVDDYYGDFPIHVACRSGFVAVAKLLLDRNCNIECETKSGWRPIHCASRNGHSEIVKLLLDRNCNTECENKSGERPIHWASRKGHSEIVKLLLDR 125          

HSP 2 Score: 93.9745 bits (232), Expect = 1.205e-22
Identity = 52/112 (46.43%), Postives = 67/112 (59.82%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            NG++E V+  L      +  +  G  PIH A R+G  ++ +LLLD NCNI+C+ K   RPIHYAS  GHSEI               KSG RP+HWAS GGH EI+K+LL+R
Sbjct:   80 NGHSEIVKLLLDRNCNTECENKSGERPIHWASRKGHSEIVKLLLDRNCNIECKTKSGMRPIHYASLQGHSEI--------------DKSGKRPIHWASWGGHSEIVKLLLER 177          
BLAST of SMED30027856 vs. Planmine SMEST
Match: SMESG000043462.1 (SMESG000043462.1)

HSP 1 Score: 181.415 bits (459), Expect = 1.201e-56
Identity = 87/124 (70.16%), Postives = 100/124 (80.65%), Query Frame = 1
Query:  130 MGEFECFKEQLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            MG+F+CFKE LMNGN EGVR NLK EFVDK++D+DG+FPIHI CR GF+ V RLLLD NCNI+CE K   RPIH+AS  GH EILKLLLDRNCN ECE + G  P+H+AS  GH EILK+LLDR
Sbjct:    1 MGDFQCFKEHLMNGNVEGVRNNLKWEFVDKIVDNDGNFPIHIVCRNGFLSVVRLLLDCNCNIECEDKCGMRPIHWASENGHLEILKLLLDRNCNIECENRWGRIPIHYASGNGHSEILKLLLDR 124          

HSP 2 Score: 115.546 bits (288), Expect = 2.731e-31
Identity = 58/89 (65.17%), Postives = 66/89 (74.16%), Query Frame = 1
Query:  235 GHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKILLDR 501
            G  PIH A   G  ++ +LLLD NCNI+C+ K   RPIH+A R GHSEILKLLLDRNCN ECE KSG R VH+ASS GH EILK+LL R
Sbjct:  102 GRIPIHYASGNGHSEILKLLLDRNCNIECQDKWGNRPIHWACRNGHSEILKLLLDRNCNIECENKSGRRSVHFASSNGHSEILKLLLQR 190          

HSP 3 Score: 91.2781 bits (225), Expect = 3.863e-22
Identity = 48/109 (44.04%), Postives = 69/109 (63.30%), Query Frame = 1
Query:  166 NGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVDVARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETKSGWRPVHWASSGGHLEILKIL 492
            NG++E ++  L      +  D  G+ PIH ACR G  ++ +LLLD NCNI+CE K   R +H+AS  GHSEILKLLL R C F+C+TK G  P+  A    +++ +++L
Sbjct:  112 NGHSEILKLLLDRNCNIECQDKWGNRPIHWACRNGHSEILKLLLDRNCNIECENKSGRRSVHFASSNGHSEILKLLLQRKCRFDCKTKDGKSPLDLAKQFNNVDCVELL 220          
The following BLAST results are available for this feature:
BLAST of SMED30027856 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANK35.577e-1737.78ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494][more]
ANK19.378e-1740.91ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492][more]
ANK19.378e-1740.91ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492][more]
ANK19.383e-1740.91ankyrin 1 [Source:HGNC Symbol;Acc:HGNC:492][more]
ANK23.566e-1638.46ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493][more]
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BLAST of SMED30027856 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
unc-444.869e-1538.37AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-444.869e-1538.37AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-444.873e-1538.37AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-444.873e-1538.37AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-444.877e-1538.37AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
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BLAST of SMED30027856 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ank4.163e-1537.23gene:FBgn0011747 transcript:FBtr0089172[more]
Ank4.163e-1537.23gene:FBgn0011747 transcript:FBtr0300497[more]
Ank4.163e-1537.23gene:FBgn0011747 transcript:FBtr0089171[more]
Ank4.163e-1537.23gene:FBgn0011747 transcript:FBtr0089173[more]
Ank22.859e-1432.98gene:FBgn0261788 transcript:FBtr0334062[more]
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BLAST of SMED30027856 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ank1b6.100e-1842.35ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
ank1b6.158e-1842.35ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
BX640476.16.284e-1842.35ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:79... [more]
ank1b6.287e-1842.35ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
BX640476.16.288e-1842.35ankyrin 1, erythrocytic b [Source:NCBI gene;Acc:79... [more]
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BLAST of SMED30027856 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANK23.463e-1638.46ankyrin 2, neuronal [Source:NCBI gene;Acc:10049480... [more]
ANK23.464e-1638.46ankyrin 2, neuronal [Source:NCBI gene;Acc:10049480... [more]
ANK23.465e-1638.46ankyrin 2, neuronal [Source:NCBI gene;Acc:10049480... [more]
ANK23.481e-1638.46ankyrin 2, neuronal [Source:NCBI gene;Acc:10049480... [more]
ANK23.481e-1638.46ankyrin 2, neuronal [Source:NCBI gene;Acc:10049480... [more]
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BLAST of SMED30027856 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ank23.146e-1738.46ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025][more]
Ank23.409e-1738.46ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025][more]
Ank24.059e-1738.30ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025][more]
Ank24.130e-1738.46ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025][more]
Ank18.283e-1740.91ankyrin 1, erythroid [Source:MGI Symbol;Acc:MGI:88... [more]
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BLAST of SMED30027856 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P16157|ANK1_HUMAN4.504e-1640.91Ankyrin-1 OS=Homo sapiens OX=9606 GN=ANK1 PE=1 SV=... [more]
sp|Q02357|ANK1_MOUSE6.067e-1640.91Ankyrin-1 OS=Mus musculus OX=10090 GN=Ank1 PE=1 SV... [more]
sp|O70511|ANK3_RAT1.282e-1537.36Ankyrin-3 OS=Rattus norvegicus OX=10116 GN=Ank3 PE... [more]
sp|Q8C8R3|ANK2_MOUSE1.838e-1538.46Ankyrin-2 OS=Mus musculus OX=10090 GN=Ank2 PE=1 SV... [more]
sp|Q01484|ANK2_HUMAN1.839e-1538.46Ankyrin-2 OS=Homo sapiens OX=9606 GN=ANK2 PE=1 SV=... [more]
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BLAST of SMED30027856 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
Q16FJ01.619e-2143.96AAEL014741-PA (Fragment) OS=Aedes aegypti OX=7159 ... [more]
J9HSB73.157e-2144.00AAEL017480-PA (Fragment) OS=Aedes aegypti OX=7159 ... [more]
E3Q4M01.266e-1940.18Uncharacterized protein OS=Colletotrichum graminic... [more]
Q16FI91.484e-1942.22AAEL014742-PA (Fragment) OS=Aedes aegypti OX=7159 ... [more]
A0A090MA793.708e-1938.66WGS project CBMG000000000 data, contig CS5907-c003... [more]
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BLAST of SMED30027856 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ank1a1.414e-1740.91ankyrin 1, erythrocytic a [Source:ZFIN;Acc:ZDB-GEN... [more]
ank1b2.591e-1739.77ankyrin 1, erythrocytic b [Source:ZFIN;Acc:ZDB-GEN... [more]
ank3b6.981e-1739.33ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2][more]
ENSAMXT00000017800.21.868e-1637.37pep primary_assembly:Astyanax_mexicanus-2.0:16:210... [more]
ENSAMXT00000037173.13.417e-1637.23pep primary_assembly:Astyanax_mexicanus-2.0:16:210... [more]
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BLAST of SMED30027856 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000000095.11.366e-1635.56pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 g... [more]
ENSPMAT00000000093.11.378e-1635.56pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 g... [more]
ENSPMAT00000007449.11.216e-1433.93pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1... [more]
ENSPMAT00000007446.11.245e-1433.93pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1... [more]
ENSPMAT00000002048.12.714e-1439.13pep scaffold:Pmarinus_7.0:GL477431:30859:73495:1 g... [more]
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BLAST of SMED30027856 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 3
Match NameE-valueIdentityDescription
GDE12.319e-1137.50Glycerophosphocholine (GroPCho) phosphodiesterase;... [more]
PHO819.782e-941.07Cyclin-dependent kinase (CDK) inhibitor; regulates... [more]
NAS69.858e-732.26Evolutionarily conserved 19S regulatory particle a... [more]
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BLAST of SMED30027856 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO454859.105e-1247.27Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO460991.056e-1133.71Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO359871.894e-1132.97Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO387023.455e-1136.47Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO340344.607e-1138.04Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30027856 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000039903.13.100e-1841.11pep primary_assembly:ASM223467v1:15:18469388:18484... [more]
ank1b3.440e-1843.53ankyrin-1 [Source:NCBI gene;Acc:101157380][more]
ENSORLT00000040233.14.600e-1737.78pep primary_assembly:ASM223467v1:19:16412534:16434... [more]
ank1a7.569e-1741.18ankyrin-1 [Source:NCBI gene;Acc:101174837][more]
ankrd52a2.667e-1639.13serine/threonine-protein phosphatase 6 regulatory ... [more]
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BLAST of SMED30027856 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000010599.15.474e-85100.00SMESG000010599.1[more]
SMESG000010218.14.784e-5870.40SMESG000010218.1[more]
SMESG000010218.15.121e-5870.40SMESG000010218.1[more]
SMESG000010579.11.025e-5772.00SMESG000010579.1[more]
SMESG000043462.11.201e-5670.16SMESG000043462.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027856 ID=SMED30027856|Name=SMED30027856|organism=Schmidtea mediterranea sexual|type=transcript|length=502bp
ATTTTGAACTTACTAATTAAATAATTGTTTTTGATTCATTTTCAAAATAT
GGATTAAATAATTTATTAATTAATATGAAATTGATTAATATTTATTAATT
TACAGATAAAACAGAAAGGAAATATCGAGATGGGGGAATTTGAATGTTTC
AAGGAGCAATTGATGAATGGAAATGCAGAGGGGGTCAGGGAGAATTTGAA
GGGGGAATTTGTGGATAAAGTTATTGATGATGATGGCCATTTTCCCATTC
ACATCGCCTGCCGTGAGGGATTTGTTGATGTTGCTAGGTTATTGCTGGAT
AATAACTGTAATATTGATTGTGAGGCCAAACGAAAATGGAGACCAATTCA
TTATGCGAGCAGAGGAGGGCATTCAGAAATTTTGAAATTATTGCTTGACC
GCAATTGCAATTTTGAGTGCGAGACAAAATCGGGATGGAGACCGGTTCAT
TGGGCGAGTAGTGGAGGACATTTAGAAATATTGAAAATACTGCTTGACCG
CA
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protein sequence of SMED30027856-orf-1

>SMED30027856-orf-1 ID=SMED30027856-orf-1|Name=SMED30027856-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=124bp
MGEFECFKEQLMNGNAEGVRENLKGEFVDKVIDDDGHFPIHIACREGFVD
VARLLLDNNCNIDCEAKRKWRPIHYASRGGHSEILKLLLDRNCNFECETK
SGWRPVHWASSGGHLEILKILLDR
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000419prepharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR036770Ankyrin_rpt-contain_sf
IPR002110Ankyrin_rpt
IPR020683Ankyrin_rpt-contain_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0003824catalytic activity
GO:0004190aspartic-type endopeptidase activity
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
GO:0009116nucleoside metabolic process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002110Ankyrin repeatSMARTSM00248ANK_2acoord: 68..97
e-value: 0.013
score: 24.6
coord: 35..64
e-value: 0.0045
score: 26.2
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 101..124
score: 10.072
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 35..67
score: 11.007
IPR002110Ankyrin repeatPROSITEPS50088ANK_REPEATcoord: 68..100
score: 9.244
IPR036770Ankyrin repeat-containing domain superfamilyGENE3DG3DSA:1.25.40.20coord: 5..124
e-value: 1.2E-29
score: 105.5
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILYSSF48403Ankyrin repeatcoord: 11..123
IPR020683Ankyrin repeat-containing domainPFAMPF12796Ank_2coord: 13..95
e-value: 2.9E-13
score: 50.2
IPR020683Ankyrin repeat-containing domainPROSITEPS50297ANK_REP_REGIONcoord: 35..124
score: 27.345
NoneNo IPR availablePANTHERPTHR24178FAMILY NOT NAMEDcoord: 30..122