SMED30027845

Overview


Name	SMED30027845
Smed ID	SMED30027845
Length (bp)	3108

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30027845 (SMED30027845) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30027845 (SMED30027845) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30027845 (SMED30027845) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30027845 (SMED30027845) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Anatomical Expression

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 16

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
tail region	SMED30027845	h1SMcG0022824	SmedASXL_018222	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
tail region	SMED30027845		SmedASXL_012104	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
X2 cell	SMED30027845		SmedASXL_012104	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015
Smed sexual biotype	SMED30027845	h1SMcG0002129	Contig34301	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30027845	h1SMcG0002129	Contig34301	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30027845	h1SMcG0022949	Contig25721	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30027845	h1SMcG0022949	Contig25721	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30027845		Contig51991	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30027845		Contig51991	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30027845		Contig306	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30027845		Contig306	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30027845		Contig30367	newmark_ests	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30027845		Contig30367	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30027845		Contig26522	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30027845		Contig26522	newmark_ests	PMID:29674431 Fincher et al., 2018
parapharyngeal region	SMED30027845		dd_Smed_v6_4_1_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017

Note: Hover over icons to view figure legend

Homology

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BLAST of SMED30027845 vs. Ensembl Zebrafish
Match: BX548011.3 (pep chromosome:GRCz11:4:68562464:68565244:1 gene:ENSDARG00000113526.1 transcript:ENSDART00000192954.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX548011.3)

HSP 1 Score: 115.161 bits (287), Expect = 1.216e-27
Identity = 87/213 (40.85%), Postives = 106/213 (49.77%), Query Frame = -3

Query: 1541 RANPFTEGTDL---ICRLPLPTLF*DYRLLTLETCCGYRYGLKRNL*LLPQIFKGHQGRFGHHKNRGAFDISHPFLQIT*F*GHVYVYKEKRTLPETLDDVSEIACVTTLCCRNNQISVFKFRNVNLIPFRFRRLLNQ*LDKLSINRRLSPPSDN*VTRSLRTD*PMFKYCSHGTLLHFSLQGSHLNNCYYYQDLYP*PLHSDSRQ*LLSNHG 2170
            R  P   G+DL   + RL +PT     RL TLETCCGY YG  R+L LLP+IFKG +   G  +N GAF  + P L +  F G +   K++   P                 R  ++    F ++N  PFR                RLS P  N V  SLRTD PMF  CSHGTLLHF LQ S LN CYY+QDL+P  LH   R  L  + G
Sbjct:   59 RLKPQNYGSDLPTSLTRL-VPT---RQRLFTLETCCGYGYGPARDLHLLPRIFKGRRELTGRRRNHGAFQGAGPSLGLNPFQGALPFTKKRELFPGLTPASPGSFASPHWAPRGARLRHSGFGDLNPTPFRS--------AGGDGGHRLSLP--NSVRPSLRTDSPMFICCSHGTLLHFGLQSSRLNICYYHQDLHPRLLHPGPRPRLPRHRG 257

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BLAST of SMED30027845 vs. Ensembl Zebrafish
Match: CT583728.14 (pep chromosome:GRCz11:4:55797866:55801210:-1 gene:ENSDARG00000112047.1 transcript:ENSDART00000186943.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CT583728.14)

HSP 1 Score: 114.775 bits (286), Expect = 1.820e-27
Identity = 79/202 (39.11%), Postives = 97/202 (48.02%), Query Frame = -3

Query: 1541 TDLICRLPLPTLF*DYRLLTLETCCGYRYGLKRNL*LLPQIFKGHQGRFGHHKNRGAFDISHPFLQIT*F*GHVYVYKEKRTLPETLDDVSEIACVTTLCCRNNQISVFKFRNVNLIPFRFRRLLNQ*LDKLSINRRLSPPSDN*VTRSLRTD*PMFKYCSHGTLLHFSLQGSHLNNCYYYQDLYP*PLHSDSRQ*LLSNHG 2146
            T L C +P        RL TLETCCGY YG  R+L LLP+IFKG +   G  +N GAF  + P L +  F G +   K++   P                 R  ++    F ++N  PFR              +    P   N V  SLRTD PMF  CSHGTLLHF LQ S LN CYY+QDL+P  LH   R  L  + G
Sbjct:   69 TSLTCLVPT-----RQRLFTLETCCGYGYGPARDLHLLPRIFKGRRELTGRRRNHGAFQGAGPSLGLNPFQGALPFTKKRELFPGLTPASPGSFASPHWAPRGARLRHSGFGDLNPTPFR----------SAGGDGGHRPSLPNSVRPSLRTDSPMFICCSHGTLLHFGLQSSRLNICYYHQDLHPRLLHPGPRPRLPRHRG 255

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BLAST of SMED30027845 vs. Ensembl Zebrafish
Match: CT583728.25 (pep chromosome:GRCz11:4:55781670:55785014:-1 gene:ENSDARG00000116179.1 transcript:ENSDART00000181348.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CT583728.25)

HSP 1 Score: 114.775 bits (286), Expect = 1.820e-27
Identity = 79/202 (39.11%), Postives = 97/202 (48.02%), Query Frame = -3

Query: 1541 TDLICRLPLPTLF*DYRLLTLETCCGYRYGLKRNL*LLPQIFKGHQGRFGHHKNRGAFDISHPFLQIT*F*GHVYVYKEKRTLPETLDDVSEIACVTTLCCRNNQISVFKFRNVNLIPFRFRRLLNQ*LDKLSINRRLSPPSDN*VTRSLRTD*PMFKYCSHGTLLHFSLQGSHLNNCYYYQDLYP*PLHSDSRQ*LLSNHG 2146
            T L C +P        RL TLETCCGY YG  R+L LLP+IFKG +   G  +N GAF  + P L +  F G +   K++   P                 R  ++    F ++N  PFR              +    P   N V  SLRTD PMF  CSHGTLLHF LQ S LN CYY+QDL+P  LH   R  L  + G
Sbjct:   69 TSLTCLVPT-----RQRLFTLETCCGYGYGPARDLHLLPRIFKGRRELTGRRRNHGAFQGAGPSLGLNPFQGALPFTKKRELFPGLTPASPGSFASPHWAPRGARLRHSGFGDLNPTPFR----------SAGGDGGHRPSLPNSVRPSLRTDSPMFICCSHGTLLHFGLQSSRLNICYYHQDLHPRLLHPGPRPRLPRHRG 255

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BLAST of SMED30027845 vs. Ensembl Zebrafish
Match: CT583728.27 (pep chromosome:GRCz11:4:55813426:55829342:-1 gene:ENSDARG00000117174.1 transcript:ENSDART00000185611.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CT583728.27)

HSP 1 Score: 113.62 bits (283), Expect = 3.814e-27
Identity = 75/186 (40.32%), Postives = 92/186 (49.46%), Query Frame = -3

Query: 1541 RLLTLETCCGYRYGLKRNL*LLPQIFKGHQGRFGHHKNRGAFDISHPFLQIT*F*GHVYVYKEKRTLPETLDDVSEIACVTTLCCRNNQISVFKFRNVNLIPFRFRRLLNQ*LDKLSINRRLSPPSDN*VTRSLRTD*PMFKYCSHGTLLHFSLQGSHLNNCYYYQDLYP*PLHSDSRQ*LLSNHG 2098
            RL TLETCCGY YG  R+L LLP+IFKG +   G  +N GAF  + P L +  F G +   K++   P                 R  ++    F ++N  PFR              +    P   N V  SLRTD PMF  CSHGTLLHF LQ S LN CYY+QDL+P  LH   R  L  + G
Sbjct:   84 RLFTLETCCGYGYGPARDLHLLPRIFKGRRELTGRRRNHGAFQGAGPSLGLNPFQGALPFTKKRELFPGLTPASPGSFASPHWAPRGARLRHSGFGDLNPTPFR----------SAGGDGGHRPSLPNSVRPSLRTDSPMFICCSHGTLLHFGLQSSRLNICYYHQDLHPRLLHPGPRPRLPRHRG 259

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BLAST of SMED30027845 vs. Ensembl Zebrafish
Match: CABZ01072614.1 (pep scaffold:GRCz11:KZ115963.1:1221:1388:1 gene:ENSDARG00000110428.1 transcript:ENSDART00000181375.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CABZ01072614.1)

HSP 1 Score: 88.9669 bits (219), Expect = 2.720e-21
Identity = 44/55 (80.00%), Postives = 46/55 (83.64%), Query Frame = 1

Query: 2536 MPRHLISDVHEWINEIPTVPIYYLAKLQPRERAWKI*RGKKTLLSLTLVRLCRET 2700
            MPRHLISD HEW+NEIPTVP  YLAK QPRERAW+  RGKKTLLSLTLV  C ET
Sbjct:    1 MPRHLISDAHEWMNEIPTVPTCYLAKPQPRERAWQNQRGKKTLLSLTLVWPCEET 55

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BLAST of SMED30027845 vs. UniProt/SwissProt
Match: sp|Q8TGM7|ART2_YEAST (Putative uncharacterized protein ART2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ART2 PE=5 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.398e-13
Identity = 35/51 (68.63%), Postives = 37/51 (72.55%), Query Frame = -1

Query: 2854 VCIRTENQNQVSFSPFGRHQVSVLVELTLGHLRYHLTDVPPQSNSPPDTVL 3006
            VCI TENQNQ  F PF   ++SVL E  LGHLRY LTDVPPQ NSPPD V 
Sbjct:    2 VCIHTENQNQGGFYPFVLLEISVLHEPPLGHLRYRLTDVPPQPNSPPDNVF 52

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BLAST of SMED30027845 vs. UniProt/SwissProt
Match: sp|Q6CQE5|TAR1_KLULA (Protein TAR1 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=TAR1-A PE=4 SV=2)

HSP 1 Score: 70.8626 bits (172), Expect = 2.190e-13
Identity = 34/55 (61.82%), Postives = 41/55 (74.55%), Query Frame = -3

Query:  215 LPLSDFTYYLTLSSKFFSTFPHGTCLLSDSHKYLALDGVYHPIWTAFSNNPTHRK 379
             P ++FTY+ TL SKFFS+F H TC LS S +YLALDG+YHP+  AF NN T RK
Sbjct:    7 FPFNNFTYFFTLFSKFFSSFHHCTCSLSVSRQYLALDGIYHPLRAAFPNNSTRRK 61

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BLAST of SMED30027845 vs. UniProt/SwissProt
Match: sp|Q8TGM6|TAR1_YEAST (Protein TAR1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TAR1 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 1.339e-12
Identity = 33/55 (60.00%), Postives = 41/55 (74.55%), Query Frame = -3

Query:  215 LPLSDFTYYLTLSSKFFSTFPHGTCLLSDSHKYLALDGVYHPIWTAFSNNPTHRK 379
             P ++FTY+ TL SKFFS+F H TC LS S +YLALDG+YHP+  AF NN T R+
Sbjct:   22 FPFNNFTYFFTLFSKFFSSFHHCTCSLSVSRQYLALDGIYHPLRAAFPNNSTLRR 76

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BLAST of SMED30027845 vs. UniProt/SwissProt
Match: sp|Q3E811|RRT15_YEAST (Regulator of rDNA transcription protein 15 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RRT15 PE=3 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 2.561e-11
Identity = 32/46 (69.57%), Postives = 34/46 (73.91%), Query Frame = -1

Query: 2869 VCIRTENQNQVSFSPFGRHQVSVLVELTLGHLRYHLTDVPPQSNSP 3006
            VCI TENQNQ  F PF   ++SVL E  LGHLRY LTDVPPQ NSP
Sbjct:    2 VCIHTENQNQGDFYPFVLLEISVLHESPLGHLRYRLTDVPPQPNSP 47

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BLAST of SMED30027845 vs. TrEMBL
Match: A0A5B6TN23 (Senescence-associated protein OS=Gossypium australe OX=47621 GN=EPI10_034369 PE=4 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 1.647e-53
Identity = 79/114 (69.30%), Postives = 85/114 (74.56%), Query Frame = -3

Query: 2432 VSKETIRVVVFHCRRESPTYTTPLMSPYKVGLESSSTGSSFPAIFSKPVPLAVVSLDSR*GQ*ESR*SIHARH*LDDEAFGYLKRVIVTPAVNQRLFEFLHIDIHGTGQKSHCV 2773
            +SK T++VVVF  R  +PTY TPL S +KVGLESSSTGSSFPA  +KPVPLAVVSLDSR GQ ES           DEAFGYLKRVIVTPAV  RL EFLH DI  TGQKSHCV
Sbjct:  237 ISKITLKVVVFQFRPRAPTYPTPLKSFHKVGLESSSTGSSFPADSAKPVPLAVVSLDSRQGQWESH----------DEAFGYLKRVIVTPAVYPRLVEFLHFDIQSTGQKSHCV 340

HSP 2 Score: 99.3673 bits (246), Expect = 1.647e-53
Identity = 49/68 (72.06%), Postives = 52/68 (76.47%), Query Frame = -1

Query: 2842 LKTSKGSIGRAFTVCIRTENQNQVSFSPFGRHQVSVLVELTLGHLRYHLTDVPPQSNSPPDTVLRTAR 3045
             + SKGSIG AFTV IRT NQNQ SF PF  H++SVLVEL LGHLRY LTDVPPQ NSPPD V R  R
Sbjct:  149 FRRSKGSIGHAFTVRIRTGNQNQTSFYPFVPHEISVLVELILGHLRYLLTDVPPQPNSPPDNVFRPDR 216

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BLAST of SMED30027845 vs. TrEMBL
Match: A0A1I7WDS0 (Uncharacterized protein OS=Heterorhabditis bacteriophora OX=37862 PE=4 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 2.813e-50
Identity = 118/207 (57.00%), Postives = 129/207 (62.32%), Query Frame = -1

Query:  880 PTYDIDPV*VPRYSAIHFQGWLIRQVSCYTLLSG*RLPCPPSCCLYQPTPFVVSHERDVRHLNQMFGSSHIASSAYQKWPTRHSYSMYISNKERHTSNPFKV*E*VENISSPILLIIRFTR*NYNHKCQLS*GKLRREPATRWFD*SFAPILMSDDRFARQNRGGPPSEFPLTSTCTSIVHHLSGLYMIAQTPPRCEHL*TGRCCTL 1500
            P+Y + P  V        Q   I +VSCYTLLSG RLP PPSCCLYQPTPF+ SHER VRHLN  FGSSH ASSAYQKWPT  + S    NKE  TS P K    VE +S P  LIIRFT  N+    QLS GKLRREP       SFAPI  S DRFARQNR G P EFPL S+C+SIVHHLSG  + A   P C+    G    L
Sbjct:  107 PSYSLRPGPVTAGYRSFAQAPSIFRVSCYTLLSGYRLPWPPSCCLYQPTPFMGSHERKVRHLNPAFGSSHSASSAYQKWPTWSTRSCQQFNKETETSYPLK----VEIVSPPSPLIIRFTGYNHAPLLQLSWGKLRREP-------SFAPIPKSIDRFARQNRYGLPPEFPLASSCSSIVHHLSGRKLYALPLPICKQTRQGHGAPL 302

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BLAST of SMED30027845 vs. TrEMBL
Match: A0A1C7LLU5 (Uncharacterized protein (Fragment) OS=Grifola frondosa OX=5627 GN=A0H81_15002 PE=4 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 8.933e-47
Identity = 75/122 (61.48%), Postives = 82/122 (67.21%), Query Frame = -3

Query: 2369 RRESPTYTTPLMSPYKVGLESSSTGSSFPAIFSKPVPLAVVSLDSR*GQ*ESR*SIHARH*LDDEAFGYLKRVIVTPAVNQRLFEFLHIDIHGTGQKSHCVKAIY*PSQCFVLIRQSDSSSP 2734
            R +SPTY+TP MS + V LESSSTGSSFPA  +KPVPLAVVSLD                  +DEAFGYLKRVIVTPAV  RL EFLH DI  TGQKSHCV   + PSQC+VLIR   S  P
Sbjct:   99 RWDSPTYSTPSMSFHNVKLESSSTGSSFPADSAKPVPLAVVSLD------------------NDEAFGYLKRVIVTPAVYPRLVEFLHFDIQSTGQKSHCVNTTFWPSQCYVLIRHYGSILP 202

HSP 2 Score: 84.3445 bits (207), Expect = 8.933e-47
Identity = 41/64 (64.06%), Postives = 45/64 (70.31%), Query Frame = -1

Query: 2854 LKTSKGSIGRAFTVCIRTENQNQVSFSPFGRHQVSVLVELTLGHLRYHLTDVPPQSNSPPDTVL 3045
             + +KGSIG  F VCI TENQNQ  F PF   ++SVL E  LGHLRY LTDVPPQ NSPPD V 
Sbjct:   23 FRGTKGSIGHTFMVCIHTENQNQGDFYPFVLLEISVLDESPLGHLRYLLTDVPPQPNSPPDNVF 86

HSP 3 Score: 115.931 bits (289), Expect = 7.500e-23
Identity = 91/232 (39.22%), Postives = 106/232 (45.69%), Query Frame = -1

Query: 1066 TNYQLIDVYHLHLITELPDRLGPTNPCSNTVHMEPFSTSVFKDLXXXXXXXXXXXTRDRSTLTHASSFLATTAPSYTISHTRSV*TPTYDIDP-V*VPRYSAIHFQGWLIRQVSCYTLLSG*RLPCPPSCCLYQPTPFVVSHERDVRHLNQMFGSSHIASSAYQKWPTRHSYSMYISNKERHTSNPFKV*E*VENISSPILLIIRFTR*NYNHKCQLS*GKLRREPATRWFD 1758
            T++++     +   TELP  LG TNPC   V MEPFSTSVFK                      ASS   T+ P+Y+ +         ++ D  V V R SAIHFQG+            G RLP PPSCCL + TPFVVS ER  RHLN  FGSS IASSAYQKWPTR+S S                                              GKLR+EPATRWFD
Sbjct:  264 TDFRVAARTSVLFETELPYLLGSTNPCPTAVTMEPFSTSVFK----------------------ASSL--TSTPAYSSARH-------FNADGGVWVTRLSAIHFQGY------------GFRLPWPPSCCLDELTPFVVSDERVFRHLNPTFGSSRIASSAYQKWPTRNSQSPS--------------------------------------------GKLRQEPATRWFD 408

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BLAST of SMED30027845 vs. TrEMBL
Match: J9I0R4 (Uncharacterized protein OS=Oxytricha trifallax OX=1172189 GN=OXYTRI_13058 PE=4 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 1.146e-46
Identity = 87/164 (53.05%), Postives = 94/164 (57.32%), Query Frame = -2

Query:  876 FLMSVMLGTLTRCLVHPTSPVLLTKNGPLGIRIRCTSPIKRDILRTHLKFENKLRTFRPQYF*SFALPGKTTIT--------NASYPEGNFGGNQLLDGSISLSPLYSCPTIDLHVRTAAVLHQSFL*LQPAQA*FTIFRVST*SLKLRPDANIFRRVDVALCV 1343
            +LMSV LGTLTR   HP+SPVLLTKNGPLG RI    P   +  R        LR  R        +P  +  +          SYPEGNFGGNQLLDGSISLSPLY   TIDLHVRTA  LHQSF  L P QA FTIFRV T  L L+P       VD A C 
Sbjct:  663 YLMSVQLGTLTRRQEHPSSPVLLTKNGPLGTRIE---PPCLNQERPGFLPIQSLRIGR-----GHCIPEASNHSLYLIKLRICPSYPEGNFGGNQLLDGSISLSPLYPSSTIDLHVRTATSLHQSFPWLHPTQAQFTIFRVLTFMLVLKPFTRGSWSVDAAACA 818

HSP 2 Score: 87.8113 bits (216), Expect = 1.146e-46
Identity = 56/100 (56.00%), Postives = 62/100 (62.00%), Query Frame = -3

Query: 1400 LRTD*PMFKYCSHGTLLHFSLQGSHLNNCYYYQDLYP*PLHSDSRQ*LLSNHGTLLHDIAYSFGVNTNLRHRSGIGPTLQRHPFSGLVDSAGELLHTP*R 1699
            LR + P+   CSHG LLHF LQ SHLNNCYY+QDL+    +  SRQ L +N   LL   A            S IG TLQRHPFSGLV SAGELLHTP R
Sbjct:  569 LRIESPVTNCCSHGNLLHFGLQNSHLNNCYYHQDLHQTLFNPGSRQRLRNNAHALLLIAASL------SLQWSSIGRTLQRHPFSGLVHSAGELLHTPQR 662

HSP 3 Score: 149.443 bits (376), Expect = 1.368e-32
Identity = 125/278 (44.96%), Postives = 146/278 (52.52%), Query Frame = -1

Query: 1855 SQAQQGXXXXXXXXXXXXXXXFR*IVDRDSRNLVNPFMHVTN*MTRHLAT*RES*LLPPLTSACLNFFTLTFMALGRNHIASKPFTSHRNALF*LDSRIPLVHASSRLTVNCTMTTMNPKTHKS*PNPPINQVEKVPLQLHLGFPQNRIKT---AQFQFNRLLD*AHHTRSLEPILLPKVRI*FADFPYLHCSKTI-GCSPWRPAADIGTA*NEIYNCSLRFSRAIRGASDTTRTAVLLTFHIPFSR*PDSRDMFMYTKKRELFPKHLMTSLRSLVLP 2676
            SQAQQGLLSPL  PSPFPWL  R IVDRDS NLVNPFM VTN MTRHLAT RES LLPP T A LNFFTLTF ALGRNHI S PF  H NA F L+ R+PLV +SS   V+C+                     ++   L +G    R ++    Q Q N           L  I  P+V   F   P     +   GC PWRP A +GT   EI   SLRFS  I  A DT+++ VL   + P S              +  F +  + SL SL+LP
Sbjct:  296 SQAQQGLLSPLIVPSPFPWLWVRQIVDRDSGNLVNPFMRVTNQMTRHLATLRESQLLPPFTRAWLNFFTLTFRALGRNHIVSTPFQGHHNAWFLLNCRVPLVRSSSESIVHCS--------------------GRIAPYLPVGLSSFRARSLWGPQTQNNLTFRAGPDCPILRAIPFPEVTELFCRLPLSTLFQLARGCQPWRPDAVMGTTWCEII--SLRFSWTIVNAPDTSKSKVLFQTYNPSS-------------GQSNFRRFTLASLSSLMLP 538

HSP 4 Score: 89.7373 bits (221), Expect = 3.314e-14
Identity = 53/78 (67.95%), Postives = 58/78 (74.36%), Query Frame = -2

Query: 2838 LSDTSCFKL*RHQKDR*AALSRSVFVLKIKIK*AFPLLVDTRFLSWLSSPWDTCVTI*QMYRPSQTPHLTLSSEQLES 3071
             SDTS  KL + QKDR A LS+ VFVLKIKIK AFPLLV  RFL  LS P DTCV + QM RPSQTPHLT+SS Q+ S
Sbjct:  194 FSDTSSLKLLK-QKDRQAMLSQFVFVLKIKIKQAFPLLVYKRFLFSLSLPQDTCVIVQQMCRPSQTPHLTMSSTQISS 270

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BLAST of SMED30027845 vs. TrEMBL
Match: J9HKN0 (Uncharacterized protein OS=Oxytricha trifallax OX=1172189 GN=OXYTRI_14248 PE=4 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 1.184e-46
Identity = 87/164 (53.05%), Postives = 94/164 (57.32%), Query Frame = -2

Query:  876 FLMSVMLGTLTRCLVHPTSPVLLTKNGPLGIRIRCTSPIKRDILRTHLKFENKLRTFRPQYF*SFALPGKTTIT--------NASYPEGNFGGNQLLDGSISLSPLYSCPTIDLHVRTAAVLHQSFL*LQPAQA*FTIFRVST*SLKLRPDANIFRRVDVALCV 1343
            +LMSV LGTLTR   HP+SPVLLTKNGPLG RI    P   +  R        LR  R        +P  +  +          SYPEGNFGGNQLLDGSISLSPLY   TIDLHVRTA  LHQSF  L P QA FTIFRV T  L L+P       VD A C 
Sbjct:  663 YLMSVQLGTLTRRQEHPSSPVLLTKNGPLGTRIE---PPCLNQERPGFLPIQSLRIGR-----GHCIPEASNHSLYLIKLRICPSYPEGNFGGNQLLDGSISLSPLYPSSTIDLHVRTATSLHQSFPWLHPTQAQFTIFRVLTFMLVLKPFTRGSWSVDAAACA 818

HSP 2 Score: 87.8113 bits (216), Expect = 1.184e-46
Identity = 56/100 (56.00%), Postives = 62/100 (62.00%), Query Frame = -3

Query: 1400 LRTD*PMFKYCSHGTLLHFSLQGSHLNNCYYYQDLYP*PLHSDSRQ*LLSNHGTLLHDIAYSFGVNTNLRHRSGIGPTLQRHPFSGLVDSAGELLHTP*R 1699
            LR + P+   CSHG LLHF LQ SHLNNCYY+QDL+    +  SRQ L +N   LL   A            S IG TLQRHPFSGLV SAGELLHTP R
Sbjct:  569 LRIESPVTNCCSHGNLLHFGLQNSHLNNCYYHQDLHQTLFNPGSRQRLRNNAHALLLIAASL------SLQWSSIGRTLQRHPFSGLVHSAGELLHTPQR 662

HSP 3 Score: 149.443 bits (376), Expect = 1.182e-32
Identity = 125/278 (44.96%), Postives = 146/278 (52.52%), Query Frame = -1

Query: 1855 SQAQQGXXXXXXXXXXXXXXXFR*IVDRDSRNLVNPFMHVTN*MTRHLAT*RES*LLPPLTSACLNFFTLTFMALGRNHIASKPFTSHRNALF*LDSRIPLVHASSRLTVNCTMTTMNPKTHKS*PNPPINQVEKVPLQLHLGFPQNRIKT---AQFQFNRLLD*AHHTRSLEPILLPKVRI*FADFPYLHCSKTI-GCSPWRPAADIGTA*NEIYNCSLRFSRAIRGASDTTRTAVLLTFHIPFSR*PDSRDMFMYTKKRELFPKHLMTSLRSLVLP 2676
            SQAQQGLLSPL  PSPFPWL  R IVDRDS NLVNPFM VTN MTRHLAT RES LLPP T A LNFFTLTF ALGRNHI S PF  H NA F L+ R+PLV +SS   V+C+                     ++   L +G    R ++    Q Q N           L  I  P+V   F   P     +   GC PWRP A +GT   EI   SLRFS  I  A DT+++ VL   + P S              +  F +  + SL SL+LP
Sbjct:  296 SQAQQGLLSPLIVPSPFPWLWVRQIVDRDSGNLVNPFMRVTNQMTRHLATLRESQLLPPFTRAWLNFFTLTFRALGRNHIVSTPFQGHHNAWFLLNCRVPLVRSSSESIVHCS--------------------GRIAPYLPVGLSSFRARSLWGPQTQNNLTFRAGPDCPILRAIPFPEVTELFCRLPLSTLFQLARGCQPWRPDAVMGTTWCEII--SLRFSWTIVNAPDTSKSKVLFQTYNPSS-------------GQSNFRRFTLASLSSLMLP 538

HSP 4 Score: 89.7373 bits (221), Expect = 3.371e-14
Identity = 53/78 (67.95%), Postives = 58/78 (74.36%), Query Frame = -2

Query: 2838 LSDTSCFKL*RHQKDR*AALSRSVFVLKIKIK*AFPLLVDTRFLSWLSSPWDTCVTI*QMYRPSQTPHLTLSSEQLES 3071
             SDTS  KL + QKDR A LS+ VFVLKIKIK AFPLLV  RFL  LS P DTCV + QM RPSQTPHLT+SS Q+ S
Sbjct:  194 FSDTSSLKLLK-QKDRQAMLSQFVFVLKIKIKQAFPLLVYKRFLFSLSLPQDTCVIVQQMCRPSQTPHLTMSSTQISS 270

[Close]

BLAST of SMED30027845 vs. Ensembl Cavefish
Match: wu:fi09b08 (wu:fi09b08 [Source:ZFIN;Acc:ZDB-GENE-030131-5630])

HSP 1 Score: 92.4337 bits (228), Expect = 1.292e-22
Identity = 45/55 (81.82%), Postives = 47/55 (85.45%), Query Frame = 1

Query: 2536 MPRHLISDVHEWINEIPTVPIYYLAKLQPRERAWKI*RGKKTLLSLTLVRLCRET 2700
            MPRHLISD HEW+NEIPTVP YYLAK QPRERAW+  RGKKTLLSLTLV  C ET
Sbjct:    1 MPRHLISDAHEWMNEIPTVPTYYLAKPQPRERAWQNQRGKKTLLSLTLVWHCEET 55

[Close]

BLAST of SMED30027845 vs. Ensembl Cavefish
Match: ENSAMXT00000038176.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000544.1:4818:11684:1 gene:ENSAMXG00000035219.1 transcript:ENSAMXT00000038176.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 65.855 bits (159), Expect = 2.391e-14
Identity = 37/104 (35.58%), Postives = 52/104 (50.00%), Query Frame = -3

Query: 1787 RLLTLETCCGYRYGLKRNL*LLPQIFKGHQGRFGHHKNRGAFDISHPFLQIT*F*GHVYVYKEKRTLPETLDDVSEIACVTTLCCRNNQISVFKFRNVNLIPFR 2098
            RL TLETCCGY YG  R+L  LP+IFKG +   G  ++RGAF  + P+L +  F G +   K++   P           +     R   +    F ++N  PFR
Sbjct:   25 RLFTLETCCGYGYGPARDLHPLPRIFKGQRELTGRRQSRGAFQGAGPYLGVNPFQGALPFTKKRELFPVPPLASPGSFALPHWAPRGAYLRHSGFGDLNPTPFR 128

HSP 2 Score: 33.8834 bits (76), Expect = 2.391e-14
Identity = 16/20 (80.00%), Postives = 17/20 (85.00%), Query Frame = -2

Query: 2109 SQSFYRRYGSNLPTSLTYIV 2168
            SQS  R YGS+LPTSLTYIV
Sbjct:    1 SQSLSRSYGSDLPTSLTYIV 20

[Close]

BLAST of SMED30027845 vs. Ensembl Cavefish
Match: ENSAMXT00000056251.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000324.1:2396:8895:1 gene:ENSAMXG00000029631.1 transcript:ENSAMXT00000056251.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 63.929 bits (154), Expect = 1.290e-10
Identity = 36/104 (34.62%), Postives = 52/104 (50.00%), Query Frame = -3

Query: 1787 RLLTLETCCGYRYGLKRNL*LLPQIFKGHQGRFGHHKNRGAFDISHPFLQIT*F*GHVYVYKEKRTLPETLDDVSEIACVTTLCCRNNQISVFKFRNVNLIPFR 2098
            R +TLETCCGY YG  R+L  LP+IFKG +   G  ++RGAF  + P+L +  F G +   K++   P           +     R   +    F ++N  PFR
Sbjct:   32 RPVTLETCCGYGYGPARDLHPLPRIFKGQRELTGRRQSRGAFQGAGPYLGVNPFQGALPFTKKRELFPVPPLASPGSFALPHWAPRGAYLRHSGFGDLNPTPFR 135

[Close]

BLAST of SMED30027845 vs. Ensembl Cavefish
Match: ENSAMXT00000053994.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000656.1:21:2715:1 gene:ENSAMXG00000034184.1 transcript:ENSAMXT00000053994.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.2174 bits (121), Expect = 1.594e-6
Identity = 38/98 (38.78%), Postives = 47/98 (47.96%), Query Frame = -3

Query: 1895 PSYPVPRANPFTEGTDLICRLPLPTLF*DYRLLTLETCCGYRYGLKRNL*LLPQIFKGHQGRFGHHKNRGAFDISHPFLQIT*F*GHVYVYKEKRTLP 2188
            P+ P  RANP+ E TDL CRLPLPTLF                   R+L  LP+IFKG +   G  +    F    P  +     G   ++KEKRTLP
Sbjct:  119 PTDPALRANPYLEVTDLTCRLPLPTLF----------------QHARDLHPLPRIFKGQRELTGRRQVPRRFPGRGPLSRGEPIPGSPALHKEKRTLP 200

[Close]

BLAST of SMED30027845 vs. Ensembl Yeast
Match: YLR154W-A (Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; encoded within the 25S rRNA gene on the opposite strand [Source:SGD;Acc:S000028675])

HSP 1 Score: 69.707 bits (169), Expect = 2.013e-15
Identity = 35/51 (68.63%), Postives = 37/51 (72.55%), Query Frame = -1

Query: 2854 VCIRTENQNQVSFSPFGRHQVSVLVELTLGHLRYHLTDVPPQSNSPPDTVL 3006
            VCI TENQNQ  F PF   ++SVL E  LGHLRY LTDVPPQ NSPPD V 
Sbjct:    2 VCIHTENQNQGGFYPFVLLEISVLHEPPLGHLRYRLTDVPPQPNSPPDNVF 52

[Close]

BLAST of SMED30027845 vs. Ensembl Yeast
Match: TAR1 (Protein potentially involved in regulation of respiratory metabolism; located in the mitochondria; interacts genetically with RPO41 and physically with Coq5p; encoded within the 25S rRNA gene on the opposite strand [Source:SGD;Acc:S000028422])

HSP 1 Score: 68.9366 bits (167), Expect = 1.929e-14
Identity = 33/55 (60.00%), Postives = 41/55 (74.55%), Query Frame = -3

Query:  215 LPLSDFTYYLTLSSKFFSTFPHGTCLLSDSHKYLALDGVYHPIWTAFSNNPTHRK 379
             P ++FTY+ TL SKFFS+F H TC LS S +YLALDG+YHP+  AF NN T R+
Sbjct:   22 FPFNNFTYFFTLFSKFFSSFHHCTCSLSVSRQYLALDGIYHPLRAAFPNNSTLRR 76

[Close]

BLAST of SMED30027845 vs. Ensembl Yeast
Match: RRT15 (Putative protein of unknown function; identified by fungal homology comparisons and RT-PCR; identified in a screen for mutants with decreased levels of rDNA transcription [Source:SGD;Acc:S000028567])

HSP 1 Score: 63.5438 bits (153), Expect = 3.688e-13
Identity = 32/46 (69.57%), Postives = 34/46 (73.91%), Query Frame = -1

Query: 2869 VCIRTENQNQVSFSPFGRHQVSVLVELTLGHLRYHLTDVPPQSNSP 3006
            VCI TENQNQ  F PF   ++SVL E  LGHLRY LTDVPPQ NSP
Sbjct:    2 VCIHTENQNQGDFYPFVLLEISVLHESPLGHLRYRLTDVPPQPNSP 47

[Close]

BLAST of SMED30027845 vs. Ensembl Nematostella
Match: EDO25991 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T944])

HSP 1 Score: 107.071 bits (266), Expect = 1.658e-27
Identity = 55/77 (71.43%), Postives = 62/77 (80.52%), Query Frame = -1

Query: 2833 LQTLKTSKGSIGRAFTVCIRTENQNQVSFSPFGRHQVSVLVELTLGHLRYHLTDVPPQSNSPPDTVL---RTARKHP 3054
            L+ LKT KGSIG AFTVCI TENQNQVSF PF  H++SVL+ELTLGHLRY LTDVPPQ NS PD+V    R+AR+ P
Sbjct:   36 LKLLKT-KGSIGHAFTVCIHTENQNQVSFYPFVLHEISVLIELTLGHLRYRLTDVPPQPNSQPDSVFDTDRSARERP 111

[Close]

BLAST of SMED30027845 vs. Ensembl Nematostella
Match: EDO27487 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T944])

HSP 1 Score: 107.071 bits (266), Expect = 1.658e-27
Identity = 55/77 (71.43%), Postives = 62/77 (80.52%), Query Frame = -1

Query: 2833 LQTLKTSKGSIGRAFTVCIRTENQNQVSFSPFGRHQVSVLVELTLGHLRYHLTDVPPQSNSPPDTVL---RTARKHP 3054
            L+ LKT KGSIG AFTVCI TENQNQVSF PF  H++SVL+ELTLGHLRY LTDVPPQ NS PD+V    R+AR+ P
Sbjct:   36 LKLLKT-KGSIGHAFTVCIHTENQNQVSFYPFVLHEISVLIELTLGHLRYRLTDVPPQPNSQPDSVFDTDRSARERP 111

[Close]

BLAST of SMED30027845 vs. Ensembl Nematostella
Match: EDO25368 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T944])

HSP 1 Score: 107.071 bits (266), Expect = 1.658e-27
Identity = 55/77 (71.43%), Postives = 62/77 (80.52%), Query Frame = -1

Query: 2833 LQTLKTSKGSIGRAFTVCIRTENQNQVSFSPFGRHQVSVLVELTLGHLRYHLTDVPPQSNSPPDTVL---RTARKHP 3054
            L+ LKT KGSIG AFTVCI TENQNQVSF PF  H++SVL+ELTLGHLRY LTDVPPQ NS PD+V    R+AR+ P
Sbjct:   36 LKLLKT-KGSIGHAFTVCIHTENQNQVSFYPFVLHEISVLIELTLGHLRYRLTDVPPQPNSQPDSVFDTDRSARERP 111

[Close]

BLAST of SMED30027845 vs. Ensembl Nematostella
Match: EDO26578 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TBM8])

HSP 1 Score: 107.071 bits (266), Expect = 1.767e-27
Identity = 53/71 (74.65%), Postives = 58/71 (81.69%), Query Frame = -1

Query: 2842 LQTLKTSKGSIGRAFTVCIRTENQNQVSFSPFGRHQVSVLVELTLGHLRYHLTDVPPQSNSPPDTVLRTAR 3054
            L+ LKT KGSIG AFTVCI TENQNQVSF PF  H++SVL+ELTLGHLRY LTDVPPQ NS PD+V  T R
Sbjct:   36 LKLLKT-KGSIGHAFTVCIHTENQNQVSFYPFVLHEISVLIELTLGHLRYRLTDVPPQPNSQPDSVFDTDR 105

[Close]

BLAST of SMED30027845 vs. Ensembl Nematostella
Match: EDO26790 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TB26])

HSP 1 Score: 106.686 bits (265), Expect = 1.879e-27
Identity = 53/71 (74.65%), Postives = 58/71 (81.69%), Query Frame = -1

Query: 2842 LQTLKTSKGSIGRAFTVCIRTENQNQVSFSPFGRHQVSVLVELTLGHLRYHLTDVPPQSNSPPDTVLRTAR 3054
            L+ LKT KGSIG AFTVCI TENQNQVSF PF  H++SVL+ELTLGHLRY LTDVPPQ NS PD+V  T R
Sbjct:   21 LKLLKT-KGSIGHAFTVCIHTENQNQVSFYPFVLHEISVLIELTLGHLRYRLTDVPPQPNSQPDSVFDTDR 90

[Close]

BLAST of SMED30027845 vs. Planmine SMEST
Match: SMESG000056391.1 (SMESG000056391.1)

HSP 1 Score: 80.8777 bits (198), Expect = 4.511e-17
Identity = 53/118 (44.92%), Postives = 67/118 (56.78%), Query Frame = -1

Query: 2140 LNFFT-LTFMALGRNHIASK--PFTSHRNALF*LDSRIPLVHASSRLTVNCTMTTMNPKTHKS*PNPPINQVEKVPLQLHLGFPQNRIKTAQFQFNRLLD*AHHTRSLEPILLPKVRI 2484
            L+FFT  + + + R++   K  PF + RN         P    S+    +C    +  K      N  INQ EKVP QLH  FPQN IKTAQFQ N++L+ A+HTR LEPILLPKVRI
Sbjct:   82 LHFFTQRSIIGISRDYKFQKFIPFMARRNTR-------PET-TSTVYNDDCKFENLQSK-----QNSRINQFEKVPFQLHFIFPQNSIKTAQFQINKILNKAYHTRFLEPILLPKVRI 186

[Close]

BLAST of SMED30027845 vs. Planmine SMEST
Match: SMESG000023237.1 (SMESG000023237.1)

HSP 1 Score: 55.0694 bits (131), Expect = 2.032e-7
Identity = 26/39 (66.67%), Postives = 32/39 (82.05%), Query Frame = 2

Query: 1151 MIRSIGDEMFSTYSQTLNGFEVCLSLLEMYIEYECLVGH 1267
            MIRSIGDEM S YSQTLNGFE+CLS L++   ++CL+ H
Sbjct:    1 MIRSIGDEMLSDYSQTLNGFELCLSSLKI---HKCLIHH 36

The following BLAST results are available for this feature:

BLAST of SMED30027845 vs. Ensembl Human
BLAST of SMED30027845 vs. Ensembl Celegans
BLAST of SMED30027845 vs. Ensembl Fly
BLAST of SMED30027845 vs. Ensembl Zebrafish
BLAST of SMED30027845 vs. Ensembl Xenopus
BLAST of SMED30027845 vs. Ensembl Mouse
BLAST of SMED30027845 vs. UniProt/SwissProt
BLAST of SMED30027845 vs. TrEMBL
BLAST of SMED30027845 vs. Ensembl Cavefish
BLAST of SMED30027845 vs. Ensembl Sea Lamprey
BLAST of SMED30027845 vs. Ensembl Yeast
BLAST of SMED30027845 vs. Ensembl Nematostella
BLAST of SMED30027845 vs. Ensembl Medaka
BLAST of SMED30027845 vs. Planmine SMEST

BLAST of SMED30027845 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30027845 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30027845 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30027845 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
BX548011.3	1.216e-27	40.85	pep chromosome:GRCz11:4:68562464:68565244:1 gene:E...	[more]
CT583728.14	1.820e-27	39.11	pep chromosome:GRCz11:4:55797866:55801210:-1 gene:...	[more]
CT583728.25	1.820e-27	39.11	pep chromosome:GRCz11:4:55781670:55785014:-1 gene:...	[more]
CT583728.27	3.814e-27	40.32	pep chromosome:GRCz11:4:55813426:55829342:-1 gene:...	[more]
CABZ01072614.1	2.720e-21	80.00	pep scaffold:GRCz11:KZ115963.1:1221:1388:1 gene:EN...	[more]

BLAST of SMED30027845 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30027845 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30027845 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4


Match Name	E-value	Identity	Description
sp\|Q8TGM7\|ART2_YEAST	1.398e-13	68.63	Putative uncharacterized protein ART2 OS=Saccharom...	[more]
sp\|Q6CQE5\|TAR1_KLULA	2.190e-13	61.82	Protein TAR1 OS=Kluyveromyces lactis (strain ATCC ...	[more]
sp\|Q8TGM6\|TAR1_YEAST	1.339e-12	60.00	Protein TAR1 OS=Saccharomyces cerevisiae (strain A...	[more]
sp\|Q3E811\|RRT15_YEAST	2.561e-11	69.57	Regulator of rDNA transcription protein 15 OS=Sacc...	[more]

BLAST of SMED30027845 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A5B6TN23	1.647e-53	69.30	Senescence-associated protein OS=Gossypium austral...	[more]
A0A1I7WDS0	2.813e-50	57.00	Uncharacterized protein OS=Heterorhabditis bacteri...	[more]
A0A1C7LLU5	8.933e-47	61.48	Uncharacterized protein (Fragment) OS=Grifola fron...	[more]
J9I0R4	1.146e-46	53.05	Uncharacterized protein OS=Oxytricha trifallax OX=...	[more]
J9HKN0	1.184e-46	53.05	Uncharacterized protein OS=Oxytricha trifallax OX=...	[more]

BLAST of SMED30027845 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4


Match Name	E-value	Identity	Description
wu:fi09b08	1.292e-22	81.82	wu:fi09b08 [Source:ZFIN;Acc:ZDB-GENE-030131-5630]	[more]
ENSAMXT00000038176.1	2.391e-14	35.58	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
ENSAMXT00000056251.1	1.290e-10	34.62	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]
ENSAMXT00000053994.1	1.594e-6	38.78	pep primary_assembly:Astyanax_mexicanus-2.0:APWO02...	[more]

BLAST of SMED30027845 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30027845 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 3


Match Name	E-value	Identity	Description
YLR154W-A	2.013e-15	68.63	Dubious open reading frame; unlikely to encode a f...	[more]
TAR1	1.929e-14	60.00	Protein potentially involved in regulation of resp...	[more]
RRT15	3.688e-13	69.57	Putative protein of unknown function; identified b...	[more]

BLAST of SMED30027845 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO25991	1.658e-27	71.43	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO27487	1.658e-27	71.43	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO25368	1.658e-27	71.43	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO26578	1.767e-27	74.65	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO26790	1.879e-27	74.65	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30027845 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30027845 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2


Match Name	E-value	Identity	Description
SMESG000056391.1	4.511e-17	44.92	SMESG000056391.1	[more]
SMESG000023237.1	2.032e-7	66.67	SMESG000023237.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence

transcript sequence

>SMED30027845 ID=SMED30027845|Name=SMED30027845|organism=Schmidtea mediterranea sexual|type=transcript|length=3108bp
TCATTAAGCGGAGGAAAAGAAACTAACAAGGATTTCCCTAGTAGTGGCGA
ACGACCGGGAATTAGCCCAGCACTGAATCCTGTATCTTTGATACTAGGAG
TTGTGGTGTACAGGTTGTGTTTTTAATGGTTGAGTATACTCCAAATTCAC
TTGATTGTGATGTTGTCCATAGAGGGTGTTAGACCCGTTGGAGTATACAA
GGCTGTTATTGACACTTCCTATGAGTCGGATTGTTTGAGAATGCAGTCCA
AATTGGGTGGTAAACTCCATCTAAGGCTAAATACTTGTGCGAGTCCGATA
GTAAACAAGTACCGTGAGGGAAAGTTGAAAAGAACTTTGAAGAGAGAGTT
AAATAGTACGTGAAATCGCTTAGAGGTAAACGAGTGGAGCCGAAGCTGAT
GTATGGGATTCAGTTGATTGTGTGTTATCTTGTCTTTATTGAAATCTCTC
AAGGATTCTCTAGAGATGAGCTCGTAATATGCGATCAATGCACTTTCTAT
GCATCAAAACTGCGACCAATAGAGCTGTTTTTCTGTCGTGTTGATAAAGG
TGACCAGTTCTTCGGAATCGGTTTTATAGTTCAATGCACGGTTAAACATA
GTGTATTGGTGATTGCGATTTGCTAATTTTAGTGATTGTCAATGCTGATT
GTTTGATGGTTAGCTCGAAACGAGATAGCTGTTGAATGGTTGGTGTAGAT
GATTGCTAAAGGGTAAATCTATAGTCTGCAGTGTATATGTAGGTTACCTG
CTCGTCCCGTCTTGAAACACGGACCAAGGAGTTTTATATGATCGCGAGTC
AAAGGGCTTTACGAAACCCCATGGCGAAATTAATGTAAAGGCTTGCTTCG
GTAAGCTGTGATGTGACCTCGTGGAAACACAGAGTGCAACATCGACCCGT
CTAAAGATGTTCGCATCTGGGCGGAGTTTGAGCGATCATGTAGAGACCCG
AAAGATGGTGAACTATGCTTGTGCAGGTTGAAGTCAGAGGAAACTCTGAT
GGAGGACCGCCGCGGTTCTGACGTGCAAATCGATCGTCGGACATGAGTAT
AGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTT
TCCCTCAGGATAGCTGGCATTTGTGATTGTAGTTTTACCTGGTAAAGCGA
ATGATTAGAAGTATTGGGGACGAAATGTTCTCAACTTATTCTCAAACTTT
AAATGGGTTCGAAGTATGTCTCTCTTTATTGGAGATGTACATCGAATACG
AATGCCTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGTGGGATGAA
CCAAACATCTGGTTAAGGTGCCTAACATCACGCTCATGAGAAACCACAAA
AGGTGTTGGTTGATAAAGACAGCAGGACGGTGGACATGGAAGTCGTCATC
CGCTAAGGAGTGTGTAACAACTCACCTGCCGAATCAACCAGCCCTGAAAA
TGGATGGCGCTGTAGCGTGGGACCTATACCGGATCGATGTCGTAAGTTGG
TGTTTACACCGAACGAGTATGCGATATCGTGTAGGAGGGTGCCGTGGTTG
CTAAGAAGCTACTGGCGTGAGTCAGAGTGGAGCGGTCACGGGTACAGATC
TTGGTAGTAGTAGCAATTATTCAAGTGAGATCCTTGAAGACTGAAGTGGA
GAAGGGTTCCATGTGAACAGTATTTGAACATGGGTTAGTCGGTCCTAAGC
GATCTGGTAACTCAGTTATCAGATGGAGGTGATAGACGTCTATTAATTGA
TAGTTTGTCTAGTTATTGATTCAATAGCCTCCTGAAGCGAAAGGGAATCA
GGTTAACATTCCTGAACTTGAATACGGAAATTTGGTTGTTTCGGCAACAC
AAGGTGGTAACACAAGCGATCTCAGAGACGTCATCAAGTGTTTCGGGAAG
AGTTCTCTTTTCTTTGTATACATAAACATGTCCCTAGAATCAGGTTATCT
GGAGAAAGGGATGTGAAATGTCAAAAGCACCGCGGTTCTTGTGGTGTCCG
AAGCGCCCCTGATGGCCCTTGAAAATCTGAGGGAGCAGTTATAAATTTCG
TTTCAAGCCGTACCGATATCCGCAGCAGGTCTCCAAGGTGAGCAGCCTAT
AGTCTTAGAACAATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTACCT
TCGGTAAAAGGATTGGCTCTAGGGACCGGGTATGATGGGCTTAATCAAGA
AGTCGATTGAATTGGAATTGGGCTGTTTTGATTCTGTTTTGGGGAAACCC
TAGATGGAGTTGAAGTGGAACTTTTTCGACTTGATTGATGGGCGGGTTAG
GCTATGATTTGTGAGTTTTCGGATTCATGGTCGTCATTGTGCAATTAACG
GTCAACCTAGAACTGGCATGGACTAGAGGAATCCGACTGTCTAATTAAAA
CAAAGCATTGCGATGGCTAGTAAATGGCTTTGACGCAATGTGATTTCTGC
CCAGTGCCATGAATGTCAATGTGAAGAAATTCAAACAAGCGCTGGTTAAC
GGCGGGAGTAACTATGACTCTCTTCAGGTAGCCAAATGCCTCGTCATCTA
ATTAGTGACGTGCATGAATGGATTAACGAGATTCCTACTGTCCCTATCTA
CTATCTAGCGAAACTACAGCCAAGGGAACGGGCTTGGAAAATATAGCGGG
GAAAGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTAGGGAGACA
TTAGAGGTGTAGTATAAGTGGGAGACTCACGTCGACAGTGAAATACCACT
ACTCTGATCGTTTCTTTACTTACTCAGTAATGCAGTGCAGTTGATTCAGG
TCAATTTATTGTGGAGAAAAGAAAGCGATCTTCGGATGCTTTCGAGCTGT
TCTGAGGACAGTGTCAGGTGGGGAGTTTGACTGGGGCGGTACATCTGTCA
AATGGTAACGCAGGTGTCCCAAGGTGAGCTCAACCAGGACAGAAACCTGG
TGTCGACCAAAAGGGGAAAAGCTCACTTGATTTTGATTTTCAGTACGAAT
ACAGACCGTGAAAGCGCGGCCTATCGATCCTTTTGATGTCTTCAGAGTTT
GAAGCAAGAGGTGTCAGAAAGACAAATTCATTAGTGTGTTGGAGCAAATT
TATGTTAA

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000420	parapharyngeal region
PLANA:0000421	tail
PLANA:0002111	X2 cell

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SMED30027845

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 16

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