EF-hand domain-containing protein

Overview
NameEF-hand domain-containing protein
Smed IDSMED30027831
Length (bp)2407
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of EF-hand domain-containing protein (SMED30027831) t-SNE clustered cells

Violin plots show distribution of expression levels for EF-hand domain-containing protein (SMED30027831) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of EF-hand domain-containing protein (SMED30027831) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for EF-hand domain-containing protein (SMED30027831) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30027831

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
headSMED30027831SMESG000032513.1 SmedASXL_017719SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
neuronSMED30027831SMESG000032513.1 dd_Smed_v4_12629_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
epidermal cellSMED30027831SMESG000032513.1 dd_Smed_v4_12629_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
epidermal cellSMED30027831SMESG000032513.1 dd_Smed_v4_14018_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30027831SMESG000032513.1 dd_Smed_v4_12629_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
dorsal ventral margin of the whole animalSMED30027831SMESG000032513.1 dd_Smed_v4_12629_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
dorsal region of the epidermisSMED30027831SMESG000032513.1 dd_Smed_v4_12629_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30027831SMESG000032513.1 dd_Smed_v4_14018_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
dorsal ventral margin of the whole animalSMED30027831SMESG000032513.1 dd_Smed_v4_14018_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: K1QD11 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10016385 PE=4 SV=1)

HSP 1 Score: 226.868 bits (577), Expect = 7.495e-59
Identity = 121/287 (42.16%), Postives = 182/287 (63.41%), Query Frame = 2
Query: 1541 SLPEISEILQPPTPD-VMETKNELATVIRNXXXXXXXXXXXRWGAKKRDFKILRDLENEQKA----EQRKFKMEKMLEKTKVKRANDKKPI--ISVGPRFETYGFLAKYCILKKINVSLYHDTFDVCDEEKKLWLNESELKMALKGVNMNLKDYDLEYLNFVMKLCDYNLQSGADFKLFAVLAALSIQITTLDSWIKNKLGKADFKTLEKKVYLCRKLWEVNVDSSSNSIPLSALMVELRAGGVKQENIEEINQRLGWMKELNFLDFLTYIPLFILLHTSIIDNPLD 2380
            +LP ++ I +PP PD  + T    + V  N+ L      +QR   K++ FK     E+E +     E+R+ K  ++LEK K ++ N    +  I   P F+ YGFLAKYCI  +  + +Y  TF+V D+E K WLN  E  +AL+GVN  L   + EYL  ++++  YN+  GADFKLF+VLAALS +I+ +D W+K  +   DF+TLE K ++C++LWE NVD  +N I L  L +ELRAGG+ +++  E+ ++LG +K L+ LDFLTY+PLFI++H S++DNPL+
Sbjct:  648 ALPTVN-IEEPPLPDPSLLTSKPTSPVKNNRSLASPIKLQQRLINKRKSFKRDTSYEDELRRQELLEKRRQKQMELLEKMKSRQGNMSTEVEYIDGVPSFDDYGFLAKYCIFNRGMLEMYKRTFEVVDDECKGWLNGIEAMIALRGVNNRLTMQEEEYLYRILEVSGYNISKGADFKLFSVLAALSHRISAVDDWMKLLIDTMDFRTLEMKTFMCKRLWECNVDKETNRISLEQLCIELRAGGISEKHEREVREKLGHLKSLDLLDFLTYVPLFIMIHQSVVDNPLN 933          
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A267EZX6 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001008g1 PE=4 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 2.585e-55
Identity = 110/247 (44.53%), Postives = 162/247 (65.59%), Query Frame = 2
Query: 1661 RWGAKKRDFKILRDLENEQK--AEQRKFKMEKMLEKTKVKRANDKKPIISVGPRFETYGFLAKYCILKKINVSLYHDTFDVCDEEKKLWLNESELKMALKGVNMNLKDYDLEYLNFVMKLCDYNLQSGADFKLFAVLAALSIQITTLDSWIKNKLGKADFKTLEKKVYLCRKLWEVNVDSSSNSIPLSALMVELRAGGVKQENIEEINQRLGWMKELNFLDFLTYIPLFILLHTSIIDNPLDMTEKL 2395
            RWGAK+ +    R+LE E+   AE RK K E++   +++K+      +   GP +E YG+LAK+CIL + NV ++  TF   D E+K +L   E+ +ALK VN  L + +  Y+  V+++C Y L++GAD KLF ++AALS +IT+LD+W++N + +  FK L+ K++LC+ LWE  V     ++ +  L +EL AGGV  ++IEE+  RLG  +E + LDFLTYIPLFILLHTSI+DNPL  T  L
Sbjct:  613 RWGAKRINHD--RELEAERARLAELRKQKAEEI--SSRLKKLIPSATMTGDGPLYEEYGWLAKFCILNRTNVPMFMGTFRAVDIEEKGFLQPDEVLLALKAVNSQLTEAEELYIFRVLEMCGYRLKAGADSKLFCIIAALSQRITSLDTWMRNAINEISFKILDMKLFLCKSLWESCVPDGFRTMNIDQLAIELLAGGVANKHIEEVRMRLGPTREFDLLDFLTYIPLFILLHTSIVDNPLSTTRDL 855          
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: R7TZK8 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_225138 PE=4 SV=1)

HSP 1 Score: 217.238 bits (552), Expect = 3.146e-55
Identity = 121/289 (41.87%), Postives = 176/289 (60.90%), Query Frame = 2
Query: 1523 TKLDLPS-LPEISEILQPPTPDVMETKNELATV-IRNXXXXXXXXXXXRWGAKKRDFKILRDLENEQKAEQRKFKMEKMLEKTKVKRANDKKPIIS-VGPRFETYGFLAKYCILKKINVSLYHDTFDVCDEEKKLWLNESELKMALKGVNMNLKDYDLEYLNFVMKLCDYNLQSGADFKLFAVLAALSIQITTLDSWIKNKLGKADFKTLEKKVYLCRKLWEVNVDSSSNSIPLSALMVELRAGGVKQENIEEINQRLGWMKELNFLDFLTYIPLFILLHTSIIDNPLD 2380
            T++  PS LP I  +   P P +  + + L  +  RN L  L     +RWG KK+ F    +   E  AE+RK K ++ML+K K +R +D + +    GP    YG+LAKYCILKK  ++LY   F+  DE+K   LN  +  +AL+  N NL D + EY+  ++ L  Y +  G+DFKLFA+L ALSI+IT+LDSW+++ L K D + L  +V+ C+ LWE NVD  + ++ +  L VELRAGGV  ++  E+   LG    L+ LDF+TYIPLFI++H S++ NPLD
Sbjct:  756 TRVPSPSPLPSIMSMS--PLPSLTSSPSRLTPMERRNSLKKLPDWANRRWGLKKKAFDWEDERRREMMAEERKLKAQQMLDKLKGRRLHDGQLVDEEQGPSVTDYGWLAKYCILKKDQLNLYKHAFEAVDEDKSGRLNSIDTMVALRATNTNLSDCEEEYMYRMLDLIGYKISDGSDFKLFAILVALSIKITSLDSWMRSLLRKIDLRQLSHRVFKCKDLWECNVDEDTRTMSIDQLCVELRAGGVSYQHELEVRDILGPKLALDLLDFITYIPLFIMIHQSVVLNPLD 1042          
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A267DYB9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig001008g3 PE=4 SV=1)

HSP 1 Score: 215.312 bits (547), Expect = 3.604e-55
Identity = 110/247 (44.53%), Postives = 162/247 (65.59%), Query Frame = 2
Query: 1661 RWGAKKRDFKILRDLENEQK--AEQRKFKMEKMLEKTKVKRANDKKPIISVGPRFETYGFLAKYCILKKINVSLYHDTFDVCDEEKKLWLNESELKMALKGVNMNLKDYDLEYLNFVMKLCDYNLQSGADFKLFAVLAALSIQITTLDSWIKNKLGKADFKTLEKKVYLCRKLWEVNVDSSSNSIPLSALMVELRAGGVKQENIEEINQRLGWMKELNFLDFLTYIPLFILLHTSIIDNPLDMTEKL 2395
            RWGAK+ +    R+LE E+   AE RK K E++   +++K+      +   GP +E YG+LAK+CIL + NV ++  TF   D E+K +L   E+ +ALK VN  L + +  Y+  V+++C Y L++GAD KLF ++AALS +IT+LD+W++N + +  FK L+ K++LC+ LWE  V     ++ +  L +EL AGGV  ++IEE+  RLG  +E + LDFLTYIPLFILLHTSI+DNPL  T  L
Sbjct:  605 RWGAKRINHD--RELEAERARLAELRKQKAEEI--SSRLKKLIPSATMTGDGPLYEEYGWLAKFCILNRTNVPMFMGTFRAVDIEEKGFLQPDEVLLALKAVNSQLTEAEELYIFRVLEMCGYRLKAGADSKLFCIIAALSQRITSLDTWMRNAINEISFKILDMKLFLCKSLWESCVPDGFRTMNIDQLAIELLAGGVANKHIEEVRMRLGPTREFDLLDFLTYIPLFILLHTSIVDNPLSTTRDL 847          
BLAST of EF-hand domain-containing protein vs. TrEMBL
Match: A0A1I8J9I5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 214.542 bits (545), Expect = 5.628e-55
Identity = 110/247 (44.53%), Postives = 162/247 (65.59%), Query Frame = 2
Query: 1661 RWGAKKRDFKILRDLENEQK--AEQRKFKMEKMLEKTKVKRANDKKPIISVGPRFETYGFLAKYCILKKINVSLYHDTFDVCDEEKKLWLNESELKMALKGVNMNLKDYDLEYLNFVMKLCDYNLQSGADFKLFAVLAALSIQITTLDSWIKNKLGKADFKTLEKKVYLCRKLWEVNVDSSSNSIPLSALMVELRAGGVKQENIEEINQRLGWMKELNFLDFLTYIPLFILLHTSIIDNPLDMTEKL 2395
            RWGAK+ +    R+LE E+   AE RK K E++   +++K+      +   GP +E YG+LAK+CIL + NV ++  TF   D E+K +L   E+ +ALK VN  L + +  Y+  V+++C Y L++GAD KLF ++AALS +IT+LD+W++N + +  FK L+ K++LC+ LWE  V     ++ +  L +EL AGGV  ++IEE+  RLG  +E + LDFLTYIPLFILLHTSI+DNPL  T  L
Sbjct:  585 RWGAKRINHD--RELEAERARLAELRKQKAEEI--SSRLKKLIPSATMTGDGPLYEEYGWLAKFCILNRTNVPMFMGTFRAVDIEEKGFLQPDEVLLALKAVNSQLTEAEELYIFRVLEMCGYRLKAGADSKLFCIIAALSQRITSLDTWMRNAINEISFKILDMKLFLCKSLWESCVPDGFRTMNIDQLAIELLAGGVANKHIEEVRMRLGPTREFDLLDFLTYIPLFILLHTSIVDNPLSTTRDL 827          
BLAST of EF-hand domain-containing protein vs. Ensembl Nematostella
Match: EDO44314 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVX6])

HSP 1 Score: 55.0694 bits (131), Expect = 1.138e-7
Identity = 58/246 (23.58%), Postives = 104/246 (42.28%), Query Frame = 2
Query: 1814 RFETYGFLAKYCILKKINVSLYHDTFDVCDEEKKLWLNESELKMALKGV--------------------------NMNLKDYDLEYL-----NFVMKLCDYNLQSGADFKLFAVLAALSIQITTLDSWIKNKL--------GKA-------DFKTLEK-----KVYLCRKLWEVNVDSSSNSIPLSALMVELRAGGVKQ----ENIEEINQRLGWMKELNFLDFLTYIPLFILLHTSIIDNPLDMT 2386
            R  T  +LA+Y ++ +  + +Y   F V DE+++  L   ++ MAL+GV                          + N+ +  L++L       + KL +Y+  +G   KL        ++         N +        G+A       + + L+      K +    ++ V  D S+  I    L +EL+AGG+ Q      I  I Q     K+++FLD++ Y+PLF+ +H +I  N LDM+
Sbjct:  609 RDSTIDYLAQYRLVDRSKIEVYGRAFTVEDEDEEGILTYEQMLMALEGVPTITGMSRQQLNYVLQVITEFTACEVHTNVVEASLQHLLISWKENIDKLRNYSCGTGVGDKLRFTYQLQDVRSHYRTVRASNHIRPNGERPGGRAGPLSDGMETRPLQGTKQVLKRFTHNNMFYVTGDRSATFISSENLRIELKAGGLSQIQEDHVIRHIMQTSDDDKKISFLDYMAYLPLFLSIHENICANALDMS 854          
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000032513.1 (SMESG000032513.1)

HSP 1 Score: 1285.4 bits (3325), Expect = 0.000e+0
Identity = 733/766 (95.69%), Postives = 739/766 (96.48%), Query Frame = 2
Query:   98 MTLPIIETNNYYGELDETPYRLPSRFQGNYQKSAVSRSLTVKSSTRNYLCARRPNTSLSMKLFLSDEKVLKVQSNTIPNFCQMEEDSNPVRPSNIELSSRQSREQNKYSSFSRGPSYINQIDRSTVTHQAAEKLVWISELNTWLSENMANYDFLKLSKTATIHDAEVWXXXXXXXXXXXXXXXXXXXXXXMWKRKDKTQISFQTVEQENLKKKLSNREMXXXXXXXXXXXXXXXXXXKKCSENKNNITYSLNTKKFCENSVEIEIXXXXXXXXXXXXVEINRTSNTSNFKVEINKTSKNSDFNDNGNLDKCEPNNQVQHSKALIDPKIAVGTNKEALSFNISKQEQINDTEYRPAFTRSNEKVTESFLRVKDSPSKDTESLTILFNSFTELQVPIPSETVTDSIELKSMDKNLSRKFQQKHKRKDFQNSFDLPDKLKTETFLDVEEFRDFQGXXXXXXXXXXXXXXXXXXXXXXNTKLDLPSLPEISEILQPPTPDVMETKNELATVIRNXXXXXXXXXXXRWGAKKRDFKILRDLENEQKAEQRKFKMEKMLEKTKVKRANDKKPIISVGPRFETYGFLAKYCILKKINVSLYHDTFDVCDEEKKLWLNESELKMALKGVNMNLKDYDLEYLNFVMKLCDYNLQSGADFKLFAVLAALSIQITTLDSWIKNKLGKADFKTLEKKVYLCRKLWEVNVDSSSNSIPLSALMVELRAGGVKQENIEEINQRLGWMKELNFLDFLTYIPLFILLHTSIIDNPLDMTEKL 2395
            MTLPIIETNNYYGELDETPYRLPSRFQGNYQKSAVSRSLTV+SSTRNYLCARRPNTSLSMKLFLSDEKVLKVQSNTIPNFCQMEEDSNPVRPSNIELS RQS                    RSTVTHQAAEKLVWISELNTWLSENMANYDFLKLSKTATIHDAEVWKKIKIKVAKLKNIKNSEKENKNMWKRKDKTQISFQTVEQENLKKKLSNREMNLSQINNSNQNMQISIVNKKCSENKNNITYSLNTKKFCENSVEIEINKSSKNSDFNDSVEINRTSNTSNFK+EINKTSKNSDFNDNGNLDKCEPNNQ+ HSKALIDPKIAVGTNKEALSFNIS QEQINDTEYRP F RSNEKVTESFLRVKDSPSKDTESLTILFNSFTELQVPIPSET+TDSIELKSMDKNLSRKFQQK KRKDFQNSFDLPDKLKTETFLDVEEFRDFQGLLDENYV+ENEEEKIEILSDEINTKLDLPSLPEISEILQPPTPDVMETKNELATVIR+KLLPLLKLQKQRWGAKKRDFKILRDLENEQKAEQRKFKMEKMLEKTKVKRANDKKPIISVGPRFETYGFLAKYCILKKINVSLYHDTFDVCDEEKKLWLNESELKMALKGVNMNLKDYDLEYLN VMKLCDYNLQSGADFKLFAVLAALSIQITTLDSWIKNKLGKADFKTLEKKVYLCRKLWEVNVDSSSNSIPLSALMVELRAGGVKQENIEEINQRLGWMKELNFLDFLTYIPLFILLHTSIIDNPLDMTEKL
Sbjct:    1 MTLPIIETNNYYGELDETPYRLPSRFQGNYQKSAVSRSLTVRSSTRNYLCARRPNTSLSMKLFLSDEKVLKVQSNTIPNFCQMEEDSNPVRPSNIELSCRQSH-------------------RSTVTHQAAEKLVWISELNTWLSENMANYDFLKLSKTATIHDAEVWKKIKIKVAKLKNIKNSEKENKNMWKRKDKTQISFQTVEQENLKKKLSNREMNLSQINNSNQNMQISIVNKKCSENKNNITYSLNTKKFCENSVEIEINKSSKNSDFNDSVEINRTSNTSNFKIEINKTSKNSDFNDNGNLDKCEPNNQI-HSKALIDPKIAVGTNKEALSFNISNQEQINDTEYRPTFPRSNEKVTESFLRVKDSPSKDTESLTILFNSFTELQVPIPSETITDSIELKSMDKNLSRKFQQKQKRKDFQNSFDLPDKLKTETFLDVEEFRDFQGLLDENYVDENEEEKIEILSDEINTKLDLPSLPEISEILQPPTPDVMETKNELATVIRSKLLPLLKLQKQRWGAKKRDFKILRDLENEQKAEQRKFKMEKMLEKTKVKRANDKKPIISVGPRFETYGFLAKYCILKKINVSLYHDTFDVCDEEKKLWLNESELKMALKGVNMNLKDYDLEYLNLVMKLCDYNLQSGADFKLFAVLAALSIQITTLDSWIKNKLGKADFKTLEKKVYLCRKLWEVNVDSSSNSIPLSALMVELRAGGVKQENIEEINQRLGWMKELNFLDFLTYIPLFILLHTSIIDNPLDMTEKL 746          
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000029294.1 (SMESG000029294.1)

HSP 1 Score: 90.1225 bits (222), Expect = 3.016e-18
Identity = 48/134 (35.82%), Postives = 84/134 (62.69%), Query Frame = 2
Query: 1997 NFVMKLCDYNLQSGADFKLFAVLAALSIQITTLDSWIKNKLGKADFKTLEKKVYLCRKLWEVN--VDSSSNSIPLSALMVELRAGGVKQENIEEINQRL--GWMKELNFLDFLTYIPLFILLHTSIIDNPLDMT 2386
            +F++   D N  +   F++F+V+AAL  ++T LDS+ ++ L  +D   +E+K+   R L+ +N   D   N I L ++ ++L+AGG+  +  E +  +L    + E++FLDFL+YIPLFI  H +I++NPLD +
Sbjct:  742 DFILLALDINKHTEITFRMFSVIAALGDRMTKLDSYCRDLLEISDLLDIERKLKFYRDLFNMNEISDHDPNYIKLESISIDLKAGGLNDDQQEFVLSKLRPNEILEISFLDFLSYIPLFISYHDNILNNPLDTS 875          
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000029294.1 (SMESG000029294.1)

HSP 1 Score: 88.9669 bits (219), Expect = 9.772e-18
Identity = 48/134 (35.82%), Postives = 84/134 (62.69%), Query Frame = 2
Query: 1997 NFVMKLCDYNLQSGADFKLFAVLAALSIQITTLDSWIKNKLGKADFKTLEKKVYLCRKLWEVN--VDSSSNSIPLSALMVELRAGGVKQENIEEINQRL--GWMKELNFLDFLTYIPLFILLHTSIIDNPLDMT 2386
            +F++   D N  +   F++F+V+AAL  ++T LDS+ ++ L  +D   +E+K+   R L+ +N   D   N I L ++ ++L+AGG+  +  E +  +L    + E++FLDFL+YIPLFI  H +I++NPLD +
Sbjct: 1286 DFILLALDINKHTEITFRMFSVIAALGDRMTKLDSYCRDLLEISDLLDIERKLKFYRDLFNMNEISDHDPNYIKLESISIDLKAGGLNDDQQEFVLSKLRPNEILEISFLDFLSYIPLFISYHDNILNNPLDTS 1419          
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000029294.1 (SMESG000029294.1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.043e-17
Identity = 48/134 (35.82%), Postives = 84/134 (62.69%), Query Frame = 2
Query: 1997 NFVMKLCDYNLQSGADFKLFAVLAALSIQITTLDSWIKNKLGKADFKTLEKKVYLCRKLWEVN--VDSSSNSIPLSALMVELRAGGVKQENIEEINQRL--GWMKELNFLDFLTYIPLFILLHTSIIDNPLDMT 2386
            +F++   D N  +   F++F+V+AAL  ++T LDS+ ++ L  +D   +E+K+   R L+ +N   D   N I L ++ ++L+AGG+  +  E +  +L    + E++FLDFL+YIPLFI  H +I++NPLD +
Sbjct: 1310 DFILLALDINKHTEITFRMFSVIAALGDRMTKLDSYCRDLLEISDLLDIERKLKFYRDLFNMNEISDHDPNYIKLESISIDLKAGGLNDDQQEFVLSKLRPNEILEISFLDFLSYIPLFISYHDNILNNPLDTS 1443          
BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Match: SMESG000063750.1 (SMESG000063750.1)

HSP 1 Score: 77.411 bits (189), Expect = 3.130e-14
Identity = 55/172 (31.98%), Postives = 97/172 (56.40%), Query Frame = 2
Query: 1901 DEEKKLWLNESELKMALKGVNMNL-KDYDLEYLNFVMKLCDYNLQSGADFKLFAVLAALSIQITTLDSWIKNKLGKADFKTLEKKVYLCRKLWEVNVDSSSN------SIPLSALMVELRAGGVKQENIEEINQRL-----GWMKELNFLDFLTYIPLFILLHTSIIDNPLD 2380
            D++K++ ++   L+ AL+ VN  L    +  Y+  ++KL  +      + + F+ +AALS ++T L+ + K  L K DF  L+ K+   +KL+  N+ SS N      ++ +  L +EL AGG+ +  +  I  +      G+   ++FLD+LTYIPLF+ +H  II++PLD
Sbjct:  882 DQDKEISIDN--LESALQVVNRQLLSKNEFNYIYHILKLPHH---PRINMQTFSCIAALSEKVTHLEPFTKRLLNKFDFNALDTKMEKAKKLF--NLMSSRNDELPDGTVSVKNLAIELMAGGIPENIVNTIIHKFDRENKGY---VDFLDYLTYIPLFVEIHERIINDPLD 1043          
The following BLAST results are available for this feature:
BLAST of EF-hand domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
K1QD117.495e-5942.16Uncharacterized protein OS=Crassostrea gigas OX=29... [more]
A0A267EZX62.585e-5544.53Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
R7TZK83.146e-5541.87Uncharacterized protein OS=Capitella teleta OX=283... [more]
A0A267DYB93.604e-5544.53Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8J9I55.628e-5544.53Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of EF-hand domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO443141.138e-723.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of EF-hand domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of EF-hand domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000032513.10.000e+095.69SMESG000032513.1[more]
SMESG000029294.13.016e-1835.82SMESG000029294.1[more]
SMESG000029294.19.772e-1835.82SMESG000029294.1[more]
SMESG000029294.11.043e-1735.82SMESG000029294.1[more]
SMESG000063750.13.130e-1431.98SMESG000063750.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027831 ID=SMED30027831|Name=EF-hand domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2407bp
TGATAATCTACTCATTGAAAAATAGTTCTGTGAACTACATATTCTGACTT
CAGTCAAGCGTTTTGTGAGATAAATAAATGTAGTCATTAATAAGAGAATG
ACGCTTCCGATAATAGAAACGAACAATTATTATGGAGAATTGGACGAGAC
CCCTTATAGGTTACCATCGCGTTTTCAAGGCAATTATCAGAAATCAGCTG
TTTCAAGATCGTTAACTGTAAAATCTTCGACGCGGAATTATTTATGTGCA
AGGAGACCAAATACGAGTTTATCTATGAAACTATTCTTGTCAGATGAAAA
GGTGCTCAAAGTTCAAAGTAATACCATACCAAATTTTTGTCAAATGGAAG
AAGATTCAAACCCAGTAAGGCCCTCCAACATTGAATTATCCTCTCGGCAA
TCGCGTGAGCAAAATAAATATTCATCCTTTTCCCGGGGTCCTTCATATAT
TAATCAAATAGACCGGTCAACAGTAACTCATCAAGCTGCAGAAAAACTAG
TTTGGATAAGTGAGCTAAATACATGGCTATCAGAGAATATGGCAAACTAC
GATTTTCTTAAACTATCGAAAACAGCAACAATACATGATGCCGAAGTGTG
GAAAAAAATTAAAATAAAAGTCGCGAAGTTGAAAAACATTAAAAATTCAG
AAAAAGAAAATAAAAACATGTGGAAAAGAAAAGATAAAACTCAAATCAGT
TTTCAAACTGTTGAACAAGAGAATTTAAAGAAGAAACTTTCAAATCGAGA
AATGAATTTATCGCAAATTAATAATTCCAACCAAAACATGCAAATATCAA
TTGTCAATAAAAAATGCAGTGAAAATAAAAACAATATAACATATTCCTTA
AATACAAAAAAATTCTGTGAAAATTCAGTAGAAATAGAAATAAACAAATC
CTCAAAGAATTCAGATTTTAATGATAGTGTAGAAATAAACAGAACATCAA
ACACTTCAAATTTCAAAGTAGAAATAAACAAAACCTCAAAGAACTCAGAT
TTCAATGATAATGGTAATTTAGATAAATGTGAACCCAACAATCAAGTACA
ACACTCCAAAGCTTTGATTGACCCTAAAATTGCAGTGGGCACCAACAAAG
AGGCTTTGTCATTTAACATTTCTAAGCAAGAACAGATAAATGATACCGAA
TATAGGCCCGCTTTTACCAGGTCAAATGAAAAAGTTACAGAATCGTTTTT
AAGAGTCAAAGATAGTCCATCTAAAGATACTGAATCGCTTACCATTCTGT
TCAATTCATTTACAGAATTACAAGTCCCAATCCCGTCGGAAACAGTAACA
GATTCTATTGAGTTAAAGTCAATGGATAAAAATTTGTCACGTAAATTTCA
ACAAAAGCATAAAAGAAAAGATTTTCAAAATTCATTTGATTTGCCAGATA
AATTGAAAACAGAAACCTTTCTTGATGTGGAAGAGTTCAGAGACTTTCAA
GGTTTATTGGACGAAAATTATGTTGAGGAGAACGAGGAAGAAAAGATTGA
AATTTTATCAGATGAAATAAATACAAAACTTGATCTGCCATCATTGCCTG
AAATTTCTGAAATTCTTCAACCTCCTACACCTGATGTTATGGAAACCAAA
AATGAACTAGCTACCGTTATCAGAAATAAACTGTTACCATTATTAAAACT
TCAAAAACAAAGATGGGGGGCCAAGAAACGAGATTTTAAAATCTTAAGAG
ATCTCGAAAATGAACAAAAAGCAGAACAAAGAAAATTTAAAATGGAAAAA
ATGTTAGAAAAAACAAAAGTAAAAAGAGCTAATGATAAAAAGCCGATCAT
TTCAGTTGGACCAAGATTCGAAACATACGGTTTTCTAGCAAAATATTGTA
TATTAAAGAAAATAAATGTGTCTCTTTATCATGATACATTTGATGTTTGT
GATGAAGAAAAAAAGCTATGGCTAAACGAATCTGAATTAAAAATGGCATT
GAAAGGAGTCAATATGAATCTGAAAGATTACGACCTAGAATATCTGAATT
TTGTTATGAAACTTTGTGACTACAATCTGCAATCGGGTGCTGATTTCAAG
TTGTTTGCCGTTCTTGCGGCATTATCAATTCAAATTACGACATTGGACTC
ATGGATAAAGAACAAACTGGGAAAGGCAGATTTCAAAACTTTGGAAAAAA
AGGTTTACTTATGTCGGAAGCTTTGGGAAGTCAACGTCGATTCGAGTTCC
AATTCAATTCCTTTAAGTGCTTTAATGGTTGAACTGAGAGCCGGTGGGGT
AAAACAAGAAAATATTGAAGAAATAAATCAAAGACTTGGATGGATGAAAG
AATTGAATTTTTTAGATTTTTTAACTTATATTCCTTTATTTATTTTGTTA
CATACCTCAATTATTGATAACCCTCTTGATATGACGGAAAAATTATAAAT
TTATTAT
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protein sequence of SMED30027831-orf-1

>SMED30027831-orf-1 ID=SMED30027831-orf-1|Name=SMED30027831-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=767bp
MTLPIIETNNYYGELDETPYRLPSRFQGNYQKSAVSRSLTVKSSTRNYLC
ARRPNTSLSMKLFLSDEKVLKVQSNTIPNFCQMEEDSNPVRPSNIELSSR
QSREQNKYSSFSRGPSYINQIDRSTVTHQAAEKLVWISELNTWLSENMAN
YDFLKLSKTATIHDAEVWKKIKIKVAKLKNIKNSEKENKNMWKRKDKTQI
SFQTVEQENLKKKLSNREMNLSQINNSNQNMQISIVNKKCSENKNNITYS
LNTKKFCENSVEIEINKSSKNSDFNDSVEINRTSNTSNFKVEINKTSKNS
DFNDNGNLDKCEPNNQVQHSKALIDPKIAVGTNKEALSFNISKQEQINDT
EYRPAFTRSNEKVTESFLRVKDSPSKDTESLTILFNSFTELQVPIPSETV
TDSIELKSMDKNLSRKFQQKHKRKDFQNSFDLPDKLKTETFLDVEEFRDF
QGLLDENYVEENEEEKIEILSDEINTKLDLPSLPEISEILQPPTPDVMET
KNELATVIRNKLLPLLKLQKQRWGAKKRDFKILRDLENEQKAEQRKFKME
KMLEKTKVKRANDKKPIISVGPRFETYGFLAKYCILKKINVSLYHDTFDV
CDEEKKLWLNESELKMALKGVNMNLKDYDLEYLNFVMKLCDYNLQSGADF
KLFAVLAALSIQITTLDSWIKNKLGKADFKTLEKKVYLCRKLWEVNVDSS
SNSIPLSALMVELRAGGVKQENIEEINQRLGWMKELNFLDFLTYIPLFIL
LHTSIIDNPLDMTEKL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000043dorsal ventral margin of the whole animal
PLANA:0000054dorsal region of the epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: molecular function
TermDefinition
GO:0005509calcium ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR35538FAMILY NOT NAMEDcoord: 173..764
NoneNo IPR availablePANTHERPTHR35538:SF3coord: 173..764