MFS domain-containing protein

Overview
NameMFS domain-containing protein
Smed IDSMED30027803
Length (bp)967
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30027803

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
whole organismSMED30027803h1SMcG0007977 Contig43121uc_Smed_v2PMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
whole organismSMED30027803h1SMcG0007977 Contig43121newmark_estsPMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of MFS domain-containing protein vs. TrEMBL
Match: T1G643 (MFS domain-containing protein OS=Helobdella robusta OX=6412 GN=20216540 PE=4 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 8.529e-11
Identity = 50/140 (35.71%), Postives = 78/140 (55.71%), Query Frame = 2 
Query:  563 KQKSHNIFKI---FWMFLSAIFDRNLLRKWAFFGMMFSNFILYACFPIAYNNLPEYALENMVSDTFQYPLLL---GMGNCIGQISIGYLGDRPFFQSIALYIFSLTISGICCLILPTYKIYTTAYIIFSLFFGFTISANF 964
            K+K H  +++   F + L  + D ++ +   F      + +LY  + I Y  LP+ A +  +S T +  LLL   G+ + IGQI IG++GDRP   S  LYI  ++I+GI  L +P    Y   ++I+ L FGFTISAN+
Sbjct:  392 KRKHHTFYEVMNGFRIALKNMMDFSIFKSPVFICFCLHSLMLYVSYDIPYMYLPDLAKDLDISAT-KASLLLSISGISSTIGQIVIGFIGDRPKVNSRYLYISMISIAGISTLFVPIIVDYWL-FVIYCLLFGFTISANY 529
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BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A267ECZ0 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig029100g2 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.591e-10
Identity = 37/116 (31.90%), Postives = 68/116 (58.62%), Query Frame = 2 
Query:  629 LLRKWAFFGMMFSNFILYACFPIAYNNLPEYALENMVSDTFQYPL---LLGMGNCIGQISIGYLGDRPFFQSIALYIFSLTISGICCLILPTYKIYTTAYIIFSLFFGFTISANFS 967
            L   W +   + SNF+LY  + I Y  +P++A E  +SD  +  L   L+G+ N IGQI IGY+GD P   ++ LY   ++++G+   ++P +     + +++ + +GF +SAN++
Sbjct:  352 LFTSWNYLSFLASNFLLYFWYDITYTFMPDHAKEIGLSDN-EVSLIIALIGVSNTIGQIVIGYIGDFPRVNTVLLYALCMSLTGVANFLVP-WCHSLASLLVYGILYGFLVSANYA 465
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BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A267F3Q8 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig002266g2 PE=4 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 8.025e-10
Identity = 37/116 (31.90%), Postives = 68/116 (58.62%), Query Frame = 2 
Query:  629 LLRKWAFFGMMFSNFILYACFPIAYNNLPEYALENMVSDTFQYPL---LLGMGNCIGQISIGYLGDRPFFQSIALYIFSLTISGICCLILPTYKIYTTAYIIFSLFFGFTISANFS 967
            L   W +   + SNF+LY  + I Y  +P++A E  +SD  +  L   L+G+ N IGQI IGY+GD P   ++ LY   ++++G+   ++P +     + +++ + +GF +SAN++
Sbjct:  589 LFTSWNYLSFLASNFLLYFWYDITYTFMPDHAKEIGLSDG-EVSLIIALIGVSNTIGQIVIGYIGDFPRVNTVLLYALCMSLTGVANFLVP-WCHSLASLLVYGILYGFLVSANYA 702
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BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A087U7C8 (Monocarboxylate transporter 9 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_00022 PE=4 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.076e-9
Identity = 43/122 (35.25%), Postives = 70/122 (57.38%), Query Frame = 2 
Query:  614 IFDRNLLRKWAFFGMMFSNFILYACFPIAYNNLPEYALE---NMVSDTFQYPLLLGMGNCIGQISIGYLGDRPFFQSIALYIFSLTISGICCLILP-TYKIYTTAYIIFSLFFGFTISANFS 967
            +FD  + R   +     SNF+LYAC  + Y  +P++A     +M S +F   +L G+ NC+G + +G++GD+P+  S  LY   + ISG   ++LP T   Y TA ++    +GF ISAN++
Sbjct:  440 MFDFTIFRNVGYTLFCISNFLLYACVDVPYVYIPDHAHSSGTDMESASFLISIL-GVLNCLGVVIVGFIGDKPWIDSSLLYSGFILISGFSLVLLPLTINYYITASLVG--IYGFAISANYT 558
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BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A1I8G9Q6 (MFS domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.224e-9
Identity = 37/116 (31.90%), Postives = 68/116 (58.62%), Query Frame = 2 
Query:  629 LLRKWAFFGMMFSNFILYACFPIAYNNLPEYALENMVSDTFQYPL---LLGMGNCIGQISIGYLGDRPFFQSIALYIFSLTISGICCLILPTYKIYTTAYIIFSLFFGFTISANFS 967
            L   W +   + SNF+LY  + I Y  +P++A E  +SD  +  L   L+G+ N IGQI IGY+GD P   ++ LY   ++++G+   ++P +     + +++ + +GF +SAN++
Sbjct:   64 LFTSWNYLSFLASNFLLYFWYDITYTFMPDHAKEIGLSDN-EVSLIIALIGVSNTIGQIVIGYIGDFPRVNTVLLYALCMSLTGVANFLVP-WCHSLASLLVYGILYGFLVSANYA 177
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BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000023543.1 (SMESG000023543.1)

HSP 1 Score: 573.163 bits (1476), Expect = 0.000e+0
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 2 
Query:   77 KRSETYSQISEIVSSAVENEKIVDDSNKIESHEQSKNNPNVVKQLNVNSNKCQLNAQSGSLKTLXXXXXXXXXXXXXXXXPKYFVDKLEKSSSIPLIRQSIYLEGIKAYLIQDHRAIFGKRKVDKGMQITFEAANNPKNIELHHKCVTKSRFQAKYFVSLLIKQKSHNIFKIFWMFLSAIFDRNLLRKWAFFGMMFSNFILYACFPIAYNNLPEYALENMVSDTFQYPLLLGMGNCIGQISIGYLGDRPFFQSIALYIFSLTISGICCLILPTYKIYTTAYIIFSLFFGFTISANFS 967
            KRSETYSQISEIVSSAVENEKIVDDSNKIESHEQSKNNPNVVKQLNVNSNKCQLNAQSGSLKTLSIEKVSIVVKSSSCVSPKYFVDKLEKSSSIPLIRQSIYLEGIKAYLIQDHRAIFGKRKVDKGMQITFEAANNPKNIELHHKCVTKSRFQAKYFVSLLIKQKSHNIFKIFWMFLSAIFDRNLLRKWAFFGMMFSNFILYACFPIAYNNLPEYALENMVSDTFQYPLLLGMGNCIGQISIGYLGDRPFFQSIALYIFSLTISGICCLILPTYKIYTTAYIIFSLFFGFTISANFS
Sbjct:    7 KRSETYSQISEIVSSAVENEKIVDDSNKIESHEQSKNNPNVVKQLNVNSNKCQLNAQSGSLKTLSIEKVSIVVKSSSCVSPKYFVDKLEKSSSIPLIRQSIYLEGIKAYLIQDHRAIFGKRKVDKGMQITFEAANNPKNIELHHKCVTKSRFQAKYFVSLLIKQKSHNIFKIFWMFLSAIFDRNLLRKWAFFGMMFSNFILYACFPIAYNNLPEYALENMVSDTFQYPLLLGMGNCIGQISIGYLGDRPFFQSIALYIFSLTISGICCLILPTYKIYTTAYIIFSLFFGFTISANFS 303
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BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000023545.1 (SMESG000023545.1)

HSP 1 Score: 58.151 bits (139), Expect = 1.391e-9
Identity = 29/34 (85.29%), Postives = 30/34 (88.24%), Query Frame = 2 
Query:    2 VIDEQKAKLFQNEFNKFIAKVDIINKRSETYSQI 103
            VIDEQKAKLFQNEFNKFIAKVDIINK S  Y +I
Sbjct:  221 VIDEQKAKLFQNEFNKFIAKVDIINKVSILYFEI 254
[Close]
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000023545.1 (SMESG000023545.1)

HSP 1 Score: 58.151 bits (139), Expect = 1.472e-9
Identity = 29/34 (85.29%), Postives = 30/34 (88.24%), Query Frame = 2 
Query:    2 VIDEQKAKLFQNEFNKFIAKVDIINKRSETYSQI 103
            VIDEQKAKLFQNEFNKFIAKVDIINK S  Y +I
Sbjct:  220 VIDEQKAKLFQNEFNKFIAKVDIINKVSILYFEI 253
The following BLAST results are available for this feature:
BLAST of MFS domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
T1G6438.529e-1135.71MFS domain-containing protein OS=Helobdella robust... [more]
A0A267ECZ02.591e-1031.90MFS domain-containing protein OS=Macrostomum ligna... [more]
A0A267F3Q88.025e-1031.90MFS domain-containing protein OS=Macrostomum ligna... [more]
A0A087U7C81.076e-935.25Monocarboxylate transporter 9 (Fragment) OS=Stegod... [more]
A0A1I8G9Q61.224e-931.90MFS domain-containing protein OS=Macrostomum ligna... [more]
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BLAST of MFS domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000023543.10.000e+0100.00SMESG000023543.1[more]
SMESG000023545.11.391e-985.29SMESG000023545.1[more]
SMESG000023545.11.472e-985.29SMESG000023545.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027803 ID=SMED30027803|Name=MFS domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=967bp
CGTTATAGATGAACAAAAAGCCAAATTATTTCAAAATGAATTTAACAAAT
TTATTGCAAAAGTTGACATAATAAATAAAAGATCAGAAACATATTCTCAA
ATTTCAGAAATAGTCAGTAGTGCTGTTGAAAATGAGAAAATTGTGGATGA
TTCTAATAAAATTGAAAGCCATGAGCAATCGAAAAATAATCCAAATGTGG
TCAAACAACTGAATGTAAACTCAAATAAATGTCAGTTGAATGCTCAATCA
GGTTCCTTGAAAACTCTTTCAATTGAAAAGGTTTCGATTGTCGTGAAAAG
TTCAAGCTGTGTCTCGCCTAAATACTTTGTTGATAAATTAGAAAAATCTA
GTTCTATTCCACTGATCAGACAATCTATATATTTAGAAGGTATAAAGGCT
TATCTTATTCAAGATCATAGAGCAATATTTGGAAAAAGAAAAGTTGATAA
AGGCATGCAAATAACATTTGAAGCTGCAAACAATCCAAAAAATATTGAGT
TACATCATAAATGTGTGACAAAATCAAGATTTCAAGCAAAATACTTTGTC
AGCTTATTGATAAAACAAAAATCGCACAATATTTTCAAAATATTTTGGAT
GTTTCTATCTGCAATCTTTGATAGAAACCTTTTGCGCAAATGGGCATTTT
TTGGAATGATGTTCAGTAATTTCATTCTTTATGCTTGTTTTCCAATAGCA
TATAACAACCTTCCTGAGTACGCTCTTGAGAACATGGTTAGCGATACCTT
CCAATATCCTTTATTGCTAGGTATGGGAAATTGCATTGGTCAAATAAGTA
TTGGCTACTTAGGAGACAGGCCTTTCTTTCAGAGTATAGCTTTGTATATT
TTTTCACTTACTATATCCGGTATTTGTTGTTTAATTCTCCCAACTTATAA
AATATACACGACTGCCTATATAATATTCAGCCTTTTTTTCGGATTTACAA
TATCTGCAAATTTCAGT
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protein sequence of SMED30027803-orf-1

>SMED30027803-orf-1 ID=SMED30027803-orf-1|Name=SMED30027803-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=322bp
VIDEQKAKLFQNEFNKFIAKVDIINKRSETYSQISEIVSSAVENEKIVDD
SNKIESHEQSKNNPNVVKQLNVNSNKCQLNAQSGSLKTLSIEKVSIVVKS
SSCVSPKYFVDKLEKSSSIPLIRQSIYLEGIKAYLIQDHRAIFGKRKVDK
GMQITFEAANNPKNIELHHKCVTKSRFQAKYFVSLLIKQKSHNIFKIFWM
FLSAIFDRNLLRKWAFFGMMFSNFILYACFPIAYNNLPEYALENMVSDTF
QYPLLLGMGNCIGQISIGYLGDRPFFQSIALYIFSLTISGICCLILPTYK
IYTTAYIIFSLFFGFTISANFS
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0015718monocarboxylic acid transport
GO:0055085transmembrane transport
Vocabulary: molecular function
TermDefinition
GO:0022857transmembrane transporter activity
Vocabulary: INTERPRO
TermDefinition
IPR036259MFS_trans_sf
IPR020846MFS_dom
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 203..322
e-value: 1.8E-5
score: 26.1
NoneNo IPR availablePANTHERPTHR11360:SF260GON-2 EXTRAGENIC MODIFIERcoord: 130..322
NoneNo IPR availablePANTHERPTHR11360MONOCARBOXYLATE TRANSPORTERcoord: 130..322
NoneNo IPR availableCDDcd17352MFS_MCT_SLC16coord: 210..322
e-value: 3.69102E-12
score: 64.1108
NoneNo IPR availableTMHMMTMhelixcoord: 214..234
NoneNo IPR availableTMHMMTMhelixcoord: 302..321
NoneNo IPR availableTMHMMTMhelixcoord: 249..271
NoneNo IPR availableTMHMMTMhelixcoord: 278..298
IPR036259MFS transporter superfamilySUPERFAMILYSSF103473MFS general substrate transportercoord: 205..320