Non-specific serine/threonine protein kinase
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30027773 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Human
Match: STK3 (serine/threonine kinase 3 [Source:HGNC Symbol;Acc:HGNC:11406]) HSP 1 Score: 93.9745 bits (232), Expect = 6.163e-24 Identity = 51/142 (35.92%), Postives = 77/142 (54.23%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ L S + F+K CL+ NP +R +A QL+ FI
Sbjct: 171 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFI 306 HSP 2 Score: 38.891 bits (89), Expect = 6.163e-24 Identity = 36/126 (28.57%), Postives = 62/126 (49.21%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K S Q + +K+ +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E+E I L + LH ++ +HRDIK
Sbjct: 53 EVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQ-VPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIK 176
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Human
Match: STK3 (serine/threonine kinase 3 [Source:HGNC Symbol;Acc:HGNC:11406]) HSP 1 Score: 93.9745 bits (232), Expect = 6.980e-24 Identity = 51/142 (35.92%), Postives = 77/142 (54.23%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ L S + F+K CL+ NP +R +A QL+ FI
Sbjct: 143 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFI 278 HSP 2 Score: 38.5058 bits (88), Expect = 6.980e-24 Identity = 36/126 (28.57%), Postives = 62/126 (49.21%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K S Q + +K+ +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E+E I L + LH ++ +HRDIK
Sbjct: 25 EVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQ-VPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIK 148
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Human
Match: STK4 (serine/threonine kinase 4 [Source:HGNC Symbol;Acc:HGNC:11408]) HSP 1 Score: 90.8929 bits (224), Expect = 5.977e-23 Identity = 49/142 (34.51%), Postives = 76/142 (53.52%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ L S F+K CL+ +P +R +A QL+ F+
Sbjct: 146 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITAIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDNFTDFVKQCLVKSPEQRATATQLLQHPFV 281 HSP 2 Score: 38.5058 bits (88), Expect = 5.977e-23 Identity = 35/126 (27.78%), Postives = 61/126 (48.41%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G V+ K + Q +V K +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E+E I L + LH ++ +HRDIK
Sbjct: 28 EVFDVLEKLGEGSYGSVYKAIHKETGQ-IVAIKQVPVESDLQEIIKEISIMQQCDSPHVVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILQSTLKGLEYLHFMRKIHRDIK 151
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Human
Match: STK4 (serine/threonine kinase 4 [Source:HGNC Symbol;Acc:HGNC:11408]) HSP 1 Score: 90.8929 bits (224), Expect = 9.156e-23 Identity = 49/142 (34.51%), Postives = 76/142 (53.52%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ L S F+K CL+ +P +R +A QL+ F+
Sbjct: 146 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITAIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDNFTDFVKQCLVKSPEQRATATQLLQHPFV 281 HSP 2 Score: 37.7354 bits (86), Expect = 9.156e-23 Identity = 35/126 (27.78%), Postives = 61/126 (48.41%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G V+ K + Q +V K +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E+E I L + LH ++ +HRDIK
Sbjct: 28 EVFDVLEKLGEGSYGSVYKAIHKETGQ-IVAIKQVPVESDLQEIIKEISIMQQCDSPHVVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILQSTLKGLEYLHFMRKIHRDIK 151
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Human
Match: PAK1 (p21 (RAC1) activated kinase 1 [Source:HGNC Symbol;Acc:HGNC:8590]) HSP 1 Score: 78.1814 bits (191), Expect = 1.662e-21 Identity = 45/142 (31.69%), Postives = 76/142 (53.52%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I +DNI+L G+ KL DFG +T E + ++ GT +MAPE++ Y K+DIWS G++ +EM+ G PP+ + P RA+ + + + +S F+ CL ++ +R SA++L+ QF+
Sbjct: 386 IHRDIKSDNILLGMDGSVKLTDFGFCAQ---ITPE---QSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFL 521 HSP 2 Score: 46.2098 bits (108), Expect = 1.662e-21 Identity = 33/117 (28.21%), Postives = 58/117 (49.57%), Query Frame = 3
Query: 2157 GAFGKVFICQDKISSQELVLKKSTEMEFSLHRDIFIG----YRLKNSSKLFQY--SRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
GA G V+ D + QE+ +K+ ++ +++ I R + + Y S +V D + + M YL+GGSLT + + C+ E + + + L + LH Q++HRDIK
Sbjct: 279 GASGTVYTAMDVATGQEVAIKQ-MNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGD--ELWVVMEYLAGGSLTD-VVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIK 391
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Celegans
Match: max-2 (Serine/threonine-protein kinase max-2 [Source:UniProtKB/Swiss-Prot;Acc:G5EGQ3]) HSP 1 Score: 88.9669 bits (219), Expect = 1.620e-25 Identity = 44/142 (30.99%), Postives = 78/142 (54.93%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
++ I +DN++L G KL D G +I + GT +M+PEIL + Y+YK+DIWS G++ +EM+ G PP+ ++ P +AI + + + + K +S E + F+ CL+V+P++R +L+ F+
Sbjct: 273 VHRDIKSDNVLLGMNGEVKLTDMGF-------CAQIQPGSKRDTVVGTPYWMSPEILNKKQYNYKVDIWSLGIMALEMIDGEPPYLRETPLKAIYLIAQNGKPEIKQRDRLSSEFNNFLDKCLVVDPDQRADTTELLAHPFL 407 HSP 2 Score: 47.7506 bits (112), Expect = 1.620e-25 Identity = 39/128 (30.47%), Postives = 61/128 (47.66%), Query Frame = 3
Query: 2130 FDFLKEFATGAFGKVFICQ-----DKISSQELVLKKSTEMEFSLHR-DIFIGYRLKNSSKLFQY--SRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
++ K+ GA G VF+ D ++ + + K + E L + YR N L Y S +V D D + + M YL GG+LT + + + + E + + + L +H LHR IVHRDIK
Sbjct: 156 YEMKKQIGVGASGTVFVANVAGSTDVVAVKRMAFKTQPKKEMLLTEIKVMKQYRHPN---LVNYIESYLV-DADDLWVVMDYLEGGNLTDVVVKTE-LDEGQIAAVLQECLKALHFLHRHSIVHRDIK 278
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Celegans
Match: max-2 (Serine/threonine-protein kinase max-2 [Source:UniProtKB/Swiss-Prot;Acc:G5EGQ3]) HSP 1 Score: 89.3521 bits (220), Expect = 2.202e-25 Identity = 44/142 (30.99%), Postives = 78/142 (54.93%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
++ I +DN++L G KL D G +I + GT +M+PEIL + Y+YK+DIWS G++ +EM+ G PP+ ++ P +AI + + + + K +S E + F+ CL+V+P++R +L+ F+
Sbjct: 445 VHRDIKSDNVLLGMNGEVKLTDMGF-------CAQIQPGSKRDTVVGTPYWMSPEILNKKQYNYKVDIWSLGIMALEMIDGEPPYLRETPLKAIYLIAQNGKPEIKQRDRLSSEFNNFLDKCLVVDPDQRADTTELLAHPFL 579 HSP 2 Score: 46.9802 bits (110), Expect = 2.202e-25 Identity = 39/128 (30.47%), Postives = 61/128 (47.66%), Query Frame = 3
Query: 2130 FDFLKEFATGAFGKVFICQ-----DKISSQELVLKKSTEMEFSLHR-DIFIGYRLKNSSKLFQY--SRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
++ K+ GA G VF+ D ++ + + K + E L + YR N L Y S +V D D + + M YL GG+LT + + + + E + + + L +H LHR IVHRDIK
Sbjct: 328 YEMKKQIGVGASGTVFVANVAGSTDVVAVKRMAFKTQPKKEMLLTEIKVMKQYRHPN---LVNYIESYLV-DADDLWVVMDYLEGGNLTDVVVKTE-LDEGQIAAVLQECLKALHFLHRHSIVHRDIK 450
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Celegans
Match: max-2 (Serine/threonine-protein kinase max-2 [Source:UniProtKB/Swiss-Prot;Acc:G5EGQ3]) HSP 1 Score: 89.3521 bits (220), Expect = 2.310e-25 Identity = 44/142 (30.99%), Postives = 78/142 (54.93%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
++ I +DN++L G KL D G +I + GT +M+PEIL + Y+YK+DIWS G++ +EM+ G PP+ ++ P +AI + + + + K +S E + F+ CL+V+P++R +L+ F+
Sbjct: 496 VHRDIKSDNVLLGMNGEVKLTDMGF-------CAQIQPGSKRDTVVGTPYWMSPEILNKKQYNYKVDIWSLGIMALEMIDGEPPYLRETPLKAIYLIAQNGKPEIKQRDRLSSEFNNFLDKCLVVDPDQRADTTELLAHPFL 630 HSP 2 Score: 46.9802 bits (110), Expect = 2.310e-25 Identity = 39/128 (30.47%), Postives = 61/128 (47.66%), Query Frame = 3
Query: 2130 FDFLKEFATGAFGKVFICQ-----DKISSQELVLKKSTEMEFSLHR-DIFIGYRLKNSSKLFQY--SRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
++ K+ GA G VF+ D ++ + + K + E L + YR N L Y S +V D D + + M YL GG+LT + + + + E + + + L +H LHR IVHRDIK
Sbjct: 379 YEMKKQIGVGASGTVFVANVAGSTDVVAVKRMAFKTQPKKEMLLTEIKVMKQYRHPN---LVNYIESYLV-DADDLWVVMDYLEGGNLTDVVVKTE-LDEGQIAAVLQECLKALHFLHRHSIVHRDIK 501
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Celegans
Match: max-2 (Serine/threonine-protein kinase max-2 [Source:UniProtKB/Swiss-Prot;Acc:G5EGQ3]) HSP 1 Score: 89.3521 bits (220), Expect = 2.407e-25 Identity = 44/142 (30.99%), Postives = 78/142 (54.93%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
++ I +DN++L G KL D G +I + GT +M+PEIL + Y+YK+DIWS G++ +EM+ G PP+ ++ P +AI + + + + K +S E + F+ CL+V+P++R +L+ F+
Sbjct: 493 VHRDIKSDNVLLGMNGEVKLTDMGF-------CAQIQPGSKRDTVVGTPYWMSPEILNKKQYNYKVDIWSLGIMALEMIDGEPPYLRETPLKAIYLIAQNGKPEIKQRDRLSSEFNNFLDKCLVVDPDQRADTTELLAHPFL 627 HSP 2 Score: 46.9802 bits (110), Expect = 2.407e-25 Identity = 39/128 (30.47%), Postives = 61/128 (47.66%), Query Frame = 3
Query: 2130 FDFLKEFATGAFGKVFICQ-----DKISSQELVLKKSTEMEFSLHR-DIFIGYRLKNSSKLFQY--SRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
++ K+ GA G VF+ D ++ + + K + E L + YR N L Y S +V D D + + M YL GG+LT + + + + E + + + L +H LHR IVHRDIK
Sbjct: 376 YEMKKQIGVGASGTVFVANVAGSTDVVAVKRMAFKTQPKKEMLLTEIKVMKQYRHPN---LVNYIESYLV-DADDLWVVMDYLEGGNLTDVVVKTE-LDEGQIAAVLQECLKALHFLHRHSIVHRDIK 498
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Celegans
Match: max-2 (Serine/threonine-protein kinase max-2 [Source:UniProtKB/Swiss-Prot;Acc:G5EGQ3]) HSP 1 Score: 88.5817 bits (218), Expect = 2.509e-25 Identity = 43/142 (30.28%), Postives = 79/142 (55.63%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
++ I +DN++L G KL D G ++ + + GT +M+PEIL + Y+YK+DIWS G++ +EM+ G PP+ ++ P +AI + + + + K +S E + F+ CL+V+P++R +L+ F+
Sbjct: 116 VHRDIKSDNVLLGMNGEVKLTDMGFCAQIQPGS-------KRDTVVGTPYWMSPEILNKKQYNYKVDIWSLGIMALEMIDGEPPYLRETPLKAIYLIAQNGKPEIKQRDRLSSEFNNFLDKCLVVDPDQRADTTELLAHPFL 250 HSP 2 Score: 47.3654 bits (111), Expect = 2.509e-25 Identity = 39/124 (31.45%), Postives = 59/124 (47.58%), Query Frame = 3
Query: 2142 KEFATGAFGKVFICQ-----DKISSQELVLKKSTEMEFSLHR-DIFIGYRLKNSSKLFQY--SRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
K+ GA G VF+ D ++ + + K + E L + YR N L Y S +V D D + + M YL GG+LT + + + + E + + + L +H LHR IVHRDIK
Sbjct: 3 KQIGVGASGTVFVANVAGSTDVVAVKRMAFKTQPKKEMLLTEIKVMKQYRHPN---LVNYIESYLV-DADDLWVVMDYLEGGNLTDVVVKTE-LDEGQIAAVLQECLKALHFLHRHSIVHRDIK 121
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Fly
Match: mbt (gene:FBgn0025743 transcript:FBtr0074285) HSP 1 Score: 88.9669 bits (219), Expect = 9.997e-24 Identity = 46/142 (32.39%), Postives = 78/142 (54.93%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I +D+I+L G KL DFG + L K K+ GT +M+PE++ +PY ++DIWS G++++EMV G PPF + P +A+ ++ M K + +S L F+ L+ +P +R +A +L+ F+
Sbjct: 484 IHRDIKSDSILLAADGRVKLSDFGFC-----AQVSQELPKRKSLV-GTPYWMSPEVISRLPYGPEVDIWSLGIMVIEMVDGEPPFFNEPPLQAMRRIRDMQPPNLKNAHKVSPRLQSFLDRMLVRDPAQRATAAELLAHPFL 619 HSP 2 Score: 42.3578 bits (98), Expect = 9.997e-24 Identity = 34/129 (26.36%), Postives = 60/129 (46.51%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFS-------LHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
EN D + G+ G V I DK + +++ +KK M+ L ++ I + + + YS + + D + + M YL GG+LT + + + E + + Q L + LH ++HRDIK
Sbjct: 366 ENLDHFNKIGEGSTGTVCIATDKSTGRQVAVKK---MDLRKQQRRELLFNEVVIMRDYHHPNIVETYSSFLVN-DELWVVMEYLEGGALTDIVTHSR-MDEEQIATVCKQCLKALAYLHSQGVIHRDIK 489
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Fly
Match: inaC (gene:FBgn0004784 transcript:FBtr0087047) HSP 1 Score: 80.4925 bits (197), Expect = 1.025e-20 Identity = 54/136 (39.71%), Postives = 68/136 (50.00%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVL---ISIELHQFIKSCLIVNPNERPSA 2917
IY + DNI+LDG G+ KL+DFGLSK E VT + + + GT NYMAPEI+ PYS D WSFG++L E + G PF +K K +V S+E I S L PN R A
Sbjct: 492 IYRDLKLDNILLDGEGHVKLVDFGLSK--EGVTER---QTTRTFC-GTPNYMAPEIVSYDPYSIAADWWSFGVLLFEFMAGQAPFEGDDETTVFRNIKD------KKAVFPKHFSVEAMDIITSFLTKKPNNRLGA 615 HSP 2 Score: 40.4318 bits (93), Expect = 1.025e-20 Identity = 29/129 (22.48%), Postives = 59/129 (45.74%), Query Frame = 3
Query: 2127 NFDFLKEFATGAFGKVFICQDKISSQELVLK--------KSTEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
+F+F+K G+FGKV + + + + + +K ++ +ME ++ + + + +S + MD + M Y GG L +QQ E+ I + ++ + LH I++RD+K
Sbjct: 370 DFNFVKVIGKGSFGKVLLAERRGTDELYAVKVLRKDVIIQTDDMELPMNEKKILALSGRPPFLVSMHS-CFQTMDRLFFVMEYCKGGDLMYHMQQYGRFKESVAIFYAVEVAIALFFLHERDIIYRDLK 497
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Fly
Match: hpo (gene:FBgn0261456 transcript:FBtr0331920) HSP 1 Score: 87.4261 bits (215), Expect = 1.884e-20 Identity = 48/142 (33.80%), Postives = 75/142 (52.82%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G AKL DFG++ + + + + GT +MAPE++ EI Y DIWS G+ +EM G PP+ + P RAI + + F+ S E F+ CL+ P++R +A +L+ +FI
Sbjct: 158 IHRDIKAANILLNTEGYAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIEEIGYDCVADIWSLGITALEMAEGKPPYGEIHPMRAIFMIPQKPPPSFREPDRWSTEFIDFVSKCLVKEPDDRATATELLEHEFI 293 HSP 2 Score: 32.7278 bits (73), Expect = 1.884e-20 Identity = 34/124 (27.42%), Postives = 59/124 (47.58%), Query Frame = 3
Query: 2130 FDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
FD + + G++G V+ K SS +V K +E LH ++I I + + + Y K D + + M Y GS++ ++ ++K + E+E I L + LH + +HRDIK
Sbjct: 42 FDIMYKLGEGSYGSVYKAVHKESSS-IVAIKLVPVESDLHEIIKEISIMQQCDSPYVVRYYGSYFKQYD-LWICMEYCGAGSVSDIMRLRKKTLTEDEIATILSDTLQGLVYLHLRRKIHRDIK 163
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Fly
Match: hpo (gene:FBgn0261456 transcript:FBtr0086500) HSP 1 Score: 87.4261 bits (215), Expect = 1.884e-20 Identity = 48/142 (33.80%), Postives = 75/142 (52.82%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G AKL DFG++ + + + + GT +MAPE++ EI Y DIWS G+ +EM G PP+ + P RAI + + F+ S E F+ CL+ P++R +A +L+ +FI
Sbjct: 158 IHRDIKAANILLNTEGYAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIEEIGYDCVADIWSLGITALEMAEGKPPYGEIHPMRAIFMIPQKPPPSFREPDRWSTEFIDFVSKCLVKEPDDRATATELLEHEFI 293 HSP 2 Score: 32.7278 bits (73), Expect = 1.884e-20 Identity = 34/124 (27.42%), Postives = 59/124 (47.58%), Query Frame = 3
Query: 2130 FDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
FD + + G++G V+ K SS +V K +E LH ++I I + + + Y K D + + M Y GS++ ++ ++K + E+E I L + LH + +HRDIK
Sbjct: 42 FDIMYKLGEGSYGSVYKAVHKESSS-IVAIKLVPVESDLHEIIKEISIMQQCDSPYVVRYYGSYFKQYD-LWICMEYCGAGSVSDIMRLRKKTLTEDEIATILSDTLQGLVYLHLRRKIHRDIK 163
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Fly
Match: Pak3 (gene:FBgn0044826 transcript:FBtr0330047) HSP 1 Score: 80.1073 bits (196), Expect = 2.756e-20 Identity = 43/142 (30.28%), Postives = 73/142 (51.41%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I +DN++L G+ K+ DFG ++E ++ + GT +MAPE++ Y K+DIWS G++ +EM+ G PP+ + P RA+ + K +S L F+ CL V + R +A +L+ F+
Sbjct: 411 IHRDIKSDNVLLGMDGSVKVTDFGFCANIEG-------DEKRQTMVGTPYWMAPEVVTRKKYGKKVDIWSIGIMAIEMIEGQPPYLYETPLRALYLIAANGRPDIKSWDKLSPNLQDFLDRCLQVEVDRRATADELLSHPFL 545 HSP 2 Score: 39.2762 bits (90), Expect = 2.756e-20 Identity = 35/128 (27.34%), Postives = 61/128 (47.66%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLHRDIFIG--YRLK--NSSKLFQY--SRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E + +E GA G VFI D + ++ V K+ +M+ +D+ + LK N L + + +++ D + + M Y+ GG LT + + + E + + + L I LH I+HRDIK
Sbjct: 291 ERYKTTQEVGKGASGIVFIAAD-LQNESQVAVKTIDMKNQSSKDLILTEIRVLKDFNHKNLVNFLDAYLLEPEDQLWVVMEYMDGGPLTD-VVTETVMKERQIACVCRETLYAISFLHAKGIIHRDIK 416
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Zebrafish
Match: stk3 (serine/threonine kinase 3 (STE20 homolog, yeast) [Source:NCBI gene;Acc:324125]) HSP 1 Score: 95.1301 bits (235), Expect = 1.190e-24 Identity = 51/144 (35.42%), Postives = 78/144 (54.17%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFIIG 2950
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ S + F+K CL+ NP +R +A QL+ FI+G
Sbjct: 142 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPEHWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIVG 279 HSP 2 Score: 39.2762 bits (90), Expect = 1.190e-24 Identity = 35/126 (27.78%), Postives = 62/126 (49.21%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K S Q + +K+ +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E+E + L + LH ++ +HRDIK
Sbjct: 24 EVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQ-VPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLTEDEIATVLKSTLKGLEYLHFMRKIHRDIK 147
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Zebrafish
Match: stk3 (serine/threonine kinase 3 (STE20 homolog, yeast) [Source:NCBI gene;Acc:324125]) HSP 1 Score: 95.1301 bits (235), Expect = 1.190e-24 Identity = 51/144 (35.42%), Postives = 78/144 (54.17%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFIIG 2950
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ S + F+K CL+ NP +R +A QL+ FI+G
Sbjct: 142 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPEHWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIVG 279 HSP 2 Score: 39.2762 bits (90), Expect = 1.190e-24 Identity = 35/126 (27.78%), Postives = 62/126 (49.21%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K S Q + +K+ +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E+E + L + LH ++ +HRDIK
Sbjct: 24 EVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQ-VPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLTEDEIATVLKSTLKGLEYLHFMRKIHRDIK 147
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Zebrafish
Match: map3k2 (mitogen-activated protein kinase kinase kinase 2 [Source:ZFIN;Acc:ZDB-GENE-120215-96]) HSP 1 Score: 72.0182 bits (175), Expect = 4.206e-23 Identity = 47/144 (32.64%), Postives = 76/144 (52.78%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSV--LISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
++ I NI+ D AGN KL DFG S+ ++ TI + +K+ GT +M+PE++ Y K DIWS G +VEM+T PP+ A A+ + K+ P++ +S F+K + V +RP+A+ L+ F+
Sbjct: 478 VHRDIKGANILRDSAGNVKLGDFGASRRLQ--TICLSGTGIKSVT-GTPYWMSPEVISGEGYGRKADIWSIGCTVVEMLTQRPPW---AEFEAMAAIFKIATQPTNPTLPPHVSDHCRDFLKR-IFVETKQRPAAEDLLRHTFV 614 HSP 2 Score: 57.3806 bits (137), Expect = 4.206e-23 Identity = 40/138 (28.99%), Postives = 66/138 (47.83%), Query Frame = 3
Query: 2127 NFDFLKEFATGAFGKVFICQDKISSQELVLKK---------------STEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDM--DSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
N+ K GAFG+VF+C D + +EL +K+ + E E L +++F ++ QY ++D ++ + M Y+ GGS+ +++ + EN K + QIL + LH IVHRDIK
Sbjct: 353 NWRLGKLLGQGAFGRVFLCYDADTGRELAVKQVQFDPDSPETSKEVSALECEIQLLKNLF-------HERIVQYYGCLRDTHEKTLSIFMEYMPGGSIKDQLKSYGALTENVTRKYTRQILEGVCYLHSNMIVHRDIK 483
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Zebrafish
Match: map3k2 (mitogen-activated protein kinase kinase kinase 2 [Source:ZFIN;Acc:ZDB-GENE-120215-96]) HSP 1 Score: 72.0182 bits (175), Expect = 4.679e-23 Identity = 47/146 (32.19%), Postives = 77/146 (52.74%), Query Frame = 2
Query: 2513 LYIYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSV--LISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
+ ++ I NI+ D AGN KL DFG S+ ++ TI + +K+ GT +M+PE++ Y K DIWS G +VEM+T PP+ A A+ + K+ P++ +S F+K + V +RP+A+ L+ F+
Sbjct: 484 MIVHRDIKGANILRDSAGNVKLGDFGASRRLQ--TICLSGTGIKSVT-GTPYWMSPEVISGEGYGRKADIWSIGCTVVEMLTQRPPW---AEFEAMAAIFKIATQPTNPTLPPHVSDHCRDFLKR-IFVETKQRPAAEDLLRHTFV 622 HSP 2 Score: 57.3806 bits (137), Expect = 4.679e-23 Identity = 40/138 (28.99%), Postives = 66/138 (47.83%), Query Frame = 3
Query: 2127 NFDFLKEFATGAFGKVFICQDKISSQELVLKK---------------STEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDM--DSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
N+ K GAFG+VF+C D + +EL +K+ + E E L +++F ++ QY ++D ++ + M Y+ GGS+ +++ + EN K + QIL + LH IVHRDIK
Sbjct: 361 NWRLGKLLGQGAFGRVFLCYDADTGRELAVKQVQFDPDSPETSKEVSALECEIQLLKNLF-------HERIVQYYGCLRDTHEKTLSIFMEYMPGGSIKDQLKSYGALTENVTRKYTRQILEGVCYLHSNMIVHRDIK 491
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Zebrafish
Match: si:ch211-1i11.3 (si:ch211-1i11.3 [Source:ZFIN;Acc:ZDB-GENE-030131-8638]) HSP 1 Score: 80.4925 bits (197), Expect = 1.059e-22 Identity = 48/147 (32.65%), Postives = 78/147 (53.06%), Query Frame = 2
Query: 2519 IYFYISADNIILDG-AGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYK--IDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSV--LISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
++ I DN++++ +G K+ DFG SK + + GT+ YMAPEI+ + P Y DIWS G ++EM TG PPFH+ +A + K+ +K P+V +S + FI C NP++R +A +L+ + F+
Sbjct: 752 VHRDIKGDNVLVNTYSGVLKISDFGTSKRLAGIN------PCTETFTGTLQYMAPEIIDQGPRGYGKPADIWSLGCTIIEMATGKPPFHELGSPQAA--MFKVGMFKIHPAVPECMSEQAKDFIMCCFEPNPDKRTTASELLKNGFL 890 HSP 2 Score: 47.7506 bits (112), Expect = 1.059e-22 Identity = 41/119 (34.45%), Postives = 66/119 (55.46%), Query Frame = 3
Query: 2157 GAFGKVFICQD-----KISSQELVLKKSTEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQ-KCIHENEKIKISY--QILTQIHELHRLQIVHRDIK 2489
G +G V+ +D +I+ +E+ K ST + LH +I + RLK+ + + QY V + I++ M + GGSL+S ++ + + +NE + Y QIL + LH QIVHRDIK
Sbjct: 641 GTYGVVYAGRDLSNQVRIAIKEIPEKDSTYSQ-PLHEEIALHKRLKHRN-IVQYLGSVSEDGFIKIFMEEVPGGSLSSLLRSKWGPLKDNEATIVFYTKQILDGLKYLHDNQIVHRDIK 757
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Xenopus
Match: elp4 (elongator acetyltransferase complex subunit 4 [Source:Xenbase;Acc:XB-GENE-991975]) HSP 1 Score: 94.7449 bits (234), Expect = 3.412e-24 Identity = 52/142 (36.62%), Postives = 77/142 (54.23%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ L S E F+K CL+ NP +R +A QL+ FI
Sbjct: 144 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDEFTDFVKKCLVKNPEQRATATQLLQHPFI 279 HSP 2 Score: 38.891 bits (89), Expect = 3.412e-24 Identity = 36/126 (28.57%), Postives = 61/126 (48.41%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K S Q + +K+ +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E E I L + LH ++ +HRDIK
Sbjct: 26 EVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQ-VPVESDLQEIIKEISIMQQCDSHYVVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLTEEEIATILRSTLKGLEYLHFMRKIHRDIK 149
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Xenopus
Match: elp4 (elongator acetyltransferase complex subunit 4 [Source:Xenbase;Acc:XB-GENE-991975]) HSP 1 Score: 95.1301 bits (235), Expect = 4.115e-24 Identity = 52/142 (36.62%), Postives = 77/142 (54.23%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ L S E F+K CL+ NP +R +A QL+ FI
Sbjct: 140 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDEFTDFVKKCLVKNPEQRATATQLLQHPFI 275 HSP 2 Score: 38.5058 bits (88), Expect = 4.115e-24 Identity = 36/126 (28.57%), Postives = 61/126 (48.41%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K S Q + +K+ +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E E I L + LH ++ +HRDIK
Sbjct: 22 EVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQ-VPVESDLQEIIKEISIMQQCDSHYVVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLTEEEIATILRSTLKGLEYLHFMRKIHRDIK 145
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Xenopus
Match: elp4 (elongator acetyltransferase complex subunit 4 [Source:Xenbase;Acc:XB-GENE-991975]) HSP 1 Score: 95.1301 bits (235), Expect = 4.394e-24 Identity = 52/142 (36.62%), Postives = 77/142 (54.23%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ L S E F+K CL+ NP +R +A QL+ FI
Sbjct: 147 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDEFTDFVKKCLVKNPEQRATATQLLQHPFI 282 HSP 2 Score: 38.5058 bits (88), Expect = 4.394e-24 Identity = 36/126 (28.57%), Postives = 61/126 (48.41%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K S Q + +K+ +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E E I L + LH ++ +HRDIK
Sbjct: 29 EVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQ-VPVESDLQEIIKEISIMQQCDSHYVVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLTEEEIATILRSTLKGLEYLHFMRKIHRDIK 152
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Xenopus
Match: elp4 (elongator acetyltransferase complex subunit 4 [Source:Xenbase;Acc:XB-GENE-991975]) HSP 1 Score: 95.1301 bits (235), Expect = 4.468e-24 Identity = 52/142 (36.62%), Postives = 77/142 (54.23%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ L S E F+K CL+ NP +R +A QL+ FI
Sbjct: 142 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDEFTDFVKKCLVKNPEQRATATQLLQHPFI 277 HSP 2 Score: 38.1206 bits (87), Expect = 4.468e-24 Identity = 36/126 (28.57%), Postives = 61/126 (48.41%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K S Q + +K+ +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E E I L + LH ++ +HRDIK
Sbjct: 24 EVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQ-VPVESDLQEIIKEISIMQQCDSHYVVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLTEEEIATILRSTLKGLEYLHFMRKIHRDIK 147
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Xenopus
Match: elp4 (elongator acetyltransferase complex subunit 4 [Source:Xenbase;Acc:XB-GENE-991975]) HSP 1 Score: 95.1301 bits (235), Expect = 4.468e-24 Identity = 52/142 (36.62%), Postives = 77/142 (54.23%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ L S E F+K CL+ NP +R +A QL+ FI
Sbjct: 139 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDEFTDFVKKCLVKNPEQRATATQLLQHPFI 274 HSP 2 Score: 38.1206 bits (87), Expect = 4.468e-24 Identity = 36/126 (28.57%), Postives = 61/126 (48.41%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K S Q + +K+ +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E E I L + LH ++ +HRDIK
Sbjct: 21 EVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQ-VPVESDLQEIIKEISIMQQCDSHYVVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLTEEEIATILRSTLKGLEYLHFMRKIHRDIK 144
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Mouse
Match: Stk3 (serine/threonine kinase 3 [Source:MGI Symbol;Acc:MGI:1928487]) HSP 1 Score: 91.6633 bits (226), Expect = 9.260e-24 Identity = 50/142 (35.21%), Postives = 77/142 (54.23%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ L S + F+K CL+ +P +R +A QL+ FI
Sbjct: 141 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKSPEQRATATQLLQHPFI 276 HSP 2 Score: 39.6614 bits (91), Expect = 9.260e-24 Identity = 36/126 (28.57%), Postives = 62/126 (49.21%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K S Q + +K+ +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E+E I L + LH ++ +HRDIK
Sbjct: 23 EVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQ-VPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILKSTLKGLEYLHFMRKIHRDIK 146
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Mouse
Match: Stk3 (serine/threonine kinase 3 [Source:MGI Symbol;Acc:MGI:1928487]) HSP 1 Score: 91.6633 bits (226), Expect = 9.336e-24 Identity = 50/142 (35.21%), Postives = 77/142 (54.23%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ L S + F+K CL+ +P +R +A QL+ FI
Sbjct: 143 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKSPEQRATATQLLQHPFI 278 HSP 2 Score: 39.6614 bits (91), Expect = 9.336e-24 Identity = 36/126 (28.57%), Postives = 62/126 (49.21%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K S Q + +K+ +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E+E I L + LH ++ +HRDIK
Sbjct: 25 EVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQ-VPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILKSTLKGLEYLHFMRKIHRDIK 148
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Mouse
Match: Stk4 (serine/threonine kinase 4 [Source:MGI Symbol;Acc:MGI:1929004]) HSP 1 Score: 90.5077 bits (223), Expect = 8.529e-23 Identity = 49/142 (34.51%), Postives = 76/142 (53.52%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ L S F+K CL+ +P +R +A QL+ F+
Sbjct: 146 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITAIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDNFMDFVKQCLVKSPEQRATATQLLQHPFV 281 HSP 2 Score: 37.7354 bits (86), Expect = 8.529e-23 Identity = 35/126 (27.78%), Postives = 61/126 (48.41%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G V+ K + Q +V K +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E+E I L + LH ++ +HRDIK
Sbjct: 28 EVFDVLEKLGEGSYGSVYKAIHKETGQ-IVAIKQVPVESDLQEIIKEISIMQQCDSPHVVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILQSTLKGLEYLHFMRKIHRDIK 151
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Mouse
Match: Pak1 (p21 (RAC1) activated kinase 1 [Source:MGI Symbol;Acc:MGI:1339975]) HSP 1 Score: 78.1814 bits (191), Expect = 1.303e-21 Identity = 45/142 (31.69%), Postives = 76/142 (53.52%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I +DNI+L G+ KL DFG +T E + ++ GT +MAPE++ Y K+DIWS G++ +EM+ G PP+ + P RA+ + + + +S F+ CL ++ +R SA++L+ QF+
Sbjct: 385 IHRDIKSDNILLGMDGSVKLTDFGFCAQ---ITPE---QSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFL 520 HSP 2 Score: 46.2098 bits (108), Expect = 1.303e-21 Identity = 33/117 (28.21%), Postives = 58/117 (49.57%), Query Frame = 3
Query: 2157 GAFGKVFICQDKISSQELVLKKSTEMEFSLHRDIFIG----YRLKNSSKLFQY--SRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
GA G V+ D + QE+ +K+ ++ +++ I R + + Y S +V D + + M YL+GGSLT + + C+ E + + + L + LH Q++HRDIK
Sbjct: 278 GASGTVYTAMDVATGQEVAIKQ-MNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGD--ELWVVMEYLAGGSLTD-VVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIK 390
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Mouse
Match: Pak1 (p21 (RAC1) activated kinase 1 [Source:MGI Symbol;Acc:MGI:1339975]) HSP 1 Score: 78.1814 bits (191), Expect = 1.303e-21 Identity = 45/142 (31.69%), Postives = 76/142 (53.52%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I +DNI+L G+ KL DFG +T E + ++ GT +MAPE++ Y K+DIWS G++ +EM+ G PP+ + P RA+ + + + +S F+ CL ++ +R SA++L+ QF+
Sbjct: 385 IHRDIKSDNILLGMDGSVKLTDFGFCAQ---ITPE---QSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFL 520 HSP 2 Score: 46.2098 bits (108), Expect = 1.303e-21 Identity = 33/117 (28.21%), Postives = 58/117 (49.57%), Query Frame = 3
Query: 2157 GAFGKVFICQDKISSQELVLKKSTEMEFSLHRDIFIG----YRLKNSSKLFQY--SRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
GA G V+ D + QE+ +K+ ++ +++ I R + + Y S +V D + + M YL+GGSLT + + C+ E + + + L + LH Q++HRDIK
Sbjct: 278 GASGTVYTAMDVATGQEVAIKQ-MNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGD--ELWVVMEYLAGGSLTD-VVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIK 390
BLAST of Non-specific serine/threonine protein kinase vs. UniProt/SwissProt
Match: sp|G5EGQ3|MAX2_CAEEL (Serine/threonine-protein kinase max-2 OS=Caenorhabditis elegans OX=6239 GN=max-2 PE=1 SV=1) HSP 1 Score: 89.3521 bits (220), Expect = 2.531e-24 Identity = 44/142 (30.99%), Postives = 78/142 (54.93%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
++ I +DN++L G KL D G +I + GT +M+PEIL + Y+YK+DIWS G++ +EM+ G PP+ ++ P +AI + + + + K +S E + F+ CL+V+P++R +L+ F+
Sbjct: 493 VHRDIKSDNVLLGMNGEVKLTDMGF-------CAQIQPGSKRDTVVGTPYWMSPEILNKKQYNYKVDIWSLGIMALEMIDGEPPYLRETPLKAIYLIAQNGKPEIKQRDRLSSEFNNFLDKCLVVDPDQRADTTELLAHPFL 627 HSP 2 Score: 46.9802 bits (110), Expect = 2.531e-24 Identity = 39/128 (30.47%), Postives = 61/128 (47.66%), Query Frame = 3
Query: 2130 FDFLKEFATGAFGKVFICQ-----DKISSQELVLKKSTEMEFSLHR-DIFIGYRLKNSSKLFQY--SRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
++ K+ GA G VF+ D ++ + + K + E L + YR N L Y S +V D D + + M YL GG+LT + + + + E + + + L +H LHR IVHRDIK
Sbjct: 376 YEMKKQIGVGASGTVFVANVAGSTDVVAVKRMAFKTQPKKEMLLTEIKVMKQYRHPN---LVNYIESYLV-DADDLWVVMDYLEGGNLTDVVVKTE-LDEGQIAAVLQECLKALHFLHRHSIVHRDIK 498
BLAST of Non-specific serine/threonine protein kinase vs. UniProt/SwissProt
Match: sp|Q7ZUQ3|STK3_DANRE (Serine/threonine-protein kinase 3 OS=Danio rerio OX=7955 GN=stk3 PE=2 SV=1) HSP 1 Score: 95.1301 bits (235), Expect = 7.352e-24 Identity = 51/144 (35.42%), Postives = 78/144 (54.17%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFIIG 2950
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ S + F+K CL+ NP +R +A QL+ FI+G
Sbjct: 142 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPEHWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIVG 279 HSP 2 Score: 39.2762 bits (90), Expect = 7.352e-24 Identity = 35/126 (27.78%), Postives = 62/126 (49.21%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K S Q + +K+ +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E+E + L + LH ++ +HRDIK
Sbjct: 24 EVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQ-VPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLTEDEIATVLKSTLKGLEYLHFMRKIHRDIK 147
BLAST of Non-specific serine/threonine protein kinase vs. UniProt/SwissProt
Match: sp|Q10056|SHK2_SCHPO (Serine/threonine-protein kinase shk2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=shk2 PE=3 SV=1) HSP 1 Score: 95.1301 bits (235), Expect = 1.191e-23 Identity = 47/142 (33.10%), Postives = 80/142 (56.34%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I +DN++LD +GN K+ DFG + N T + + GT +MAPE++++ Y K+DIWS G++++EM+ PP+ ++ P RA+ + K K L+S L F+ SCL ++ R +A +L+ F+
Sbjct: 431 IHRDIKSDNVLLDNSGNIKITDFGFCARLSNRTNK------RVTMVGTPYWMAPEVVKQNEYGTKVDIWSLGIMIIEMIENEPPYLREDPIRALYLIAKNGTPTLKKPNLVSKNLKSFLNSCLTIDTIFRATAAELLTHSFL 566 HSP 2 Score: 38.5058 bits (88), Expect = 1.191e-23 Identity = 35/117 (29.91%), Postives = 56/117 (47.86%), Query Frame = 3
Query: 2157 GAFGKVFI------CQDKISSQELVLKKSTEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
G F V I CQ + +EL+L + T M S+H +I + S +V++ + + M Y++ GSLT I++ K + E + +I + I LH I+HRDIK
Sbjct: 335 GIFDSVAIKSIDLQCQTR---KELILNEITVMRESIHPNIVT----------YLDSFLVRERH-LWVVMEYMNAGSLTDIIEKSK-LTEAQIARICLETCKGIQHLHARNIIHRDIK 436
BLAST of Non-specific serine/threonine protein kinase vs. UniProt/SwissProt
Match: sp|Q802A6|STK4_SQUAC (Serine/threonine-protein kinase 3/4 OS=Squalus acanthias OX=7797 GN=STK4 PE=2 SV=1) HSP 1 Score: 93.9745 bits (232), Expect = 1.297e-23 Identity = 51/142 (35.92%), Postives = 77/142 (54.23%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ L + E F+K CL+ NP +R +A QL+ FI
Sbjct: 148 IHRDIKAGNILLNNEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGISAIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWTDEFTDFVKQCLVKNPEQRAAATQLLQHPFI 283 HSP 2 Score: 39.6614 bits (91), Expect = 1.297e-23 Identity = 36/126 (28.57%), Postives = 62/126 (49.21%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K S Q + +K+ +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E+E I L + LH ++ +HRDIK
Sbjct: 30 EVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQ-VPVESDLQEIIKEISIMQQCDSPHVVKYYGSYFKNTD-LWIVMEYCGAGSVSDLIRIRNKTLTEDEIATILQSTLKGLEYLHFMRKIHRDIK 153
BLAST of Non-specific serine/threonine protein kinase vs. UniProt/SwissProt
Match: sp|Q6IP06|STK3_XENLA (Serine/threonine-protein kinase 3 OS=Xenopus laevis OX=8355 GN=stk3 PE=2 SV=1) HSP 1 Score: 93.9745 bits (232), Expect = 2.006e-23 Identity = 51/142 (35.92%), Postives = 77/142 (54.23%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ L + E F+K CL+ NP +R +A QL+ FI
Sbjct: 142 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWTDEFTDFVKKCLVKNPEQRATATQLLQHPFI 277 HSP 2 Score: 38.891 bits (89), Expect = 2.006e-23 Identity = 36/126 (28.57%), Postives = 62/126 (49.21%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K S Q + +K+ +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E+E I L + LH ++ +HRDIK
Sbjct: 24 EVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQ-VPVESDLQEIIKEISIMQQCDSHYVVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILRSTLKGLEYLHFMRKIHRDIK 147
BLAST of Non-specific serine/threonine protein kinase vs. TrEMBL
Match: T2M6M6 (Mitogen-activated protein kinase kinase kinase 3 OS=Hydra vulgaris OX=6087 GN=MAP3K3 PE=2 SV=1) HSP 1 Score: 83.9593 bits (206), Expect = 5.825e-25 Identity = 45/142 (31.69%), Postives = 74/142 (52.11%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I NI+LD +GN KL DFG SK ++ + + + + GT +M+PE++ Y K DIWS G ++EM+T PP+ + P A+ ++ P SI +F+ C +P+ RP+A +L+ F+
Sbjct: 489 IHRDIKGANILLDSSGNCKLADFGASKQIQTIRSQTGCKSVH----GTPYWMSPEVINGAGYGRKADIWSLGCTVLEMLTTKPPWFQFEPMAALFKIATQTTIPHLPDD-SSISCKRFVDDCFKRDPSLRPNALELLSYAFV 625 HSP 2 Score: 62.003 bits (149), Expect = 5.825e-25 Identity = 42/135 (31.11%), Postives = 69/135 (51.11%), Query Frame = 3
Query: 2127 NFDF--LKEFATGAFGKVFICQDKISSQELVLK------------KSTEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
NF + +++ +G FG V++C D + +EL +K K E+ L R+I + Y+ N ++ +Y ++ SI + M Y+ GGS+ KI + + E E +QIL I+ LH I+HRDIK
Sbjct: 364 NFKWKQVRQIGSGGFGTVYLCVDLNTGKELAMKYIETGHINTAALKEVEI---LQREISL-YKTLNHERIVEYYGTIQANTSISIFMEYMEGGSIHDKISKIGALDEKETSCYCFQILEGINYLHSKNIIHRDIK 494
BLAST of Non-specific serine/threonine protein kinase vs. TrEMBL
Match: I7LT45 (Cyclin-dependent kinase-like Serine/Threonine kinase family protein OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHERM_00716030 PE=4 SV=2) HSP 1 Score: 98.9821 bits (245), Expect = 1.199e-24 Identity = 52/142 (36.62%), Postives = 84/142 (59.15%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ + +DNI+L+ G+ K+ DFG + + T E +K + GT +MAPE++ YS K+DIWS G++ +E++ G PP+ + PA+A+ + K +K + S E F+ +CL ++P ERP+AQQLI FI
Sbjct: 295 IHRDLKSDNILLNQNGDVKIADFGFATQL---TREHFSKKSQV---GTPAWMAPELILRKDYSQKVDIWSLGVICIEILEGEPPYIRDKPAKAMYYITKKKPHKLD-ATKFSQEACDFVSTCLNIDPQERPTAQQLISHPFI 429 HSP 2 Score: 46.2098 bits (108), Expect = 1.199e-24 Identity = 40/131 (30.53%), Postives = 64/131 (48.85%), Query Frame = 3
Query: 2130 FDFLKEFATGAFGKVFICQDKISSQELVLK----------KSTEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQ-KCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
+DFL + GA KV+ C++K + QE ++ + T+ME I + KN++ + QY + MD I +A+ Y++ GSLT I + E I QIL + +HR +HRD+K
Sbjct: 177 YDFLSQIGKGATCKVYRCKNKQTGQECAMRVIRMLNPDQIEKTKME------ILLMNESKNNN-IVQYYKSYIYMDVIFMAIEYMNAGSLTELIYGNFSFLSEQMASYICEQILFGLFFIHRTNQIHRDLK 300
BLAST of Non-specific serine/threonine protein kinase vs. TrEMBL
Match: T1G1N2 (Protein kinase domain-containing protein OS=Helobdella robusta OX=6412 GN=20214980 PE=4 SV=1) HSP 1 Score: 91.6633 bits (226), Expect = 2.956e-24 Identity = 53/149 (35.57%), Postives = 78/149 (52.35%), Query Frame = 2
Query: 2510 LLYIYFYISADNIILDGAGNAKLIDFGLSK---SMENVTIEILLEKLKAYA-PGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
L+ ++ I NI+ D GN KL DFG +K S+ N T ++ A GT YM+PEI+ Y K+DIWS G +VEM+TG+PP+H+ AI ++ +K S+++ F+ C +P RPSA+ L FI
Sbjct: 124 LMIVHRDIKGANILRDHEGNIKLTDFGAAKRLSSIVNKTADVSKNLTGAKTMTGTPYYMSPEIIEGKRYGRKVDIWSLGCTVVEMLTGHPPWHEYEGVAAIFKIATQPHPSYKLPPDTSLQVKDFLSCCFERDPERRPSAKILYNHMFI 272 HSP 2 Score: 52.373 bits (124), Expect = 2.956e-24 Identity = 39/131 (29.77%), Postives = 65/131 (49.62%), Query Frame = 3
Query: 2127 NFDFLKEFATGAFGKVFICQDKISSQELVLKK----------STEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
N+ K G FG+V++C D + EL +K+ S E+ +L +I + L N ++ QY K+ + + M ++ GGS+ +IQ + E+ + + Q+L + LH L IVHRDIK
Sbjct: 4 NWKKGKILGAGGFGQVYLCYDVDTGVELAVKQVNVYCQSEDISKEVR-ALQSEIKLLQDLSNP-RIVQYYGSQKEPHMLSIFMEFVPGGSVKDQIQDYGPLTESVTKRYTKQVLEGLEFLHSLMIVHRDIK 132
BLAST of Non-specific serine/threonine protein kinase vs. TrEMBL
Match: A7S4G7 (Predicted protein (Fragment) OS=Nematostella vectensis OX=45351 GN=v1g104385 PE=4 SV=1) HSP 1 Score: 81.2629 bits (199), Expect = 3.518e-24 Identity = 47/142 (33.10%), Postives = 74/142 (52.11%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
++ I NI+ D GN KL DFG SK ++ + + + GT +MAPE++ Y K D+WS G +VEM+T PP+ P A+ ++ PS L S++ +F++S L +N RPSA +L+ F+
Sbjct: 126 VHRDIKGANILRDLYGNVKLADFGASKRLQTIRSKTGFRSVH----GTPYWMAPEVINGEGYGRKADVWSVGCTVVEMLTTKPPWADFEPMAALFKIATQPTEPELPSDL-SVDAVEFVRSTLRMNSKSRPSADELLRFSFV 262 HSP 2 Score: 62.3882 bits (150), Expect = 3.518e-24 Identity = 47/130 (36.15%), Postives = 68/130 (52.31%), Query Frame = 3
Query: 2127 NFDFLKEFATGAFGKVFICQDKISSQELVLKK--------STEMEF-SLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
N+ K GAFG+V++C D + +EL +K+ ST+ E +L +I +N ++ QY I D I + M YL GGS+ I+Q ++E+ K S QIL I LH +IVHRDIK
Sbjct: 3 NWRKGKLLGAGAFGQVYMCHDLDTGRELAVKQIETGQLNSSTKNEVKALEGEIEFMKAFRNE-RIVQYYGIETDDLHIYIFMEYLPGGSIHEHIKQHGALNESLTRKYSRQILEGILYLHTNRIVHRDIK 131
BLAST of Non-specific serine/threonine protein kinase vs. TrEMBL
Match: T1EDA5 (Protein kinase domain-containing protein OS=Helobdella robusta OX=6412 GN=20194557 PE=4 SV=1) HSP 1 Score: 90.5077 bits (223), Expect = 7.819e-24 Identity = 46/138 (33.33%), Postives = 73/138 (52.90%), Query Frame = 2
Query: 2531 ISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
+ NI+ D GN KL DFG SK ++ ++ I+ + GT+ YM+PE+L Y K DIWS G +VEM+TG+PP+H+ AI ++ E + +S + F+ C + RP++ QL+ F+
Sbjct: 132 VKGANILRDNEGNVKLSDFGASKRLQTISNSIMQTQ-----TGTIYYMSPELLDGNGYGRKTDIWSLGCSVVEMLTGHPPWHQLENFAAIFKIVTSDEPGYSLPDSVSKQARDFVSECFTRSLERRPTSSQLLYHSFV 264 HSP 2 Score: 51.9878 bits (123), Expect = 7.819e-24 Identity = 35/133 (26.32%), Postives = 63/133 (47.37%), Query Frame = 3
Query: 2121 MENFDFLKEFATGAFGKVFICQDKISSQELVLKK----------STEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
M+++ + +G+FG+VF+C D + +E +K+ + + +L +I I + QY D + M Y+ GGS+ +I+Q + E I + Q+L + LH+ IVHRD+K
Sbjct: 1 MKSWKRGRLLGSGSFGQVFLCCDMETGKEYAMKQVHIFSKNSQHCSRVLSALREEINILKEGVKHPHIVQYFGCQDDGVVFSILMEYMPGGSVKDQIKQYGPLTERNTISYTKQVLLGLEYLHKKSIVHRDVK 133
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Cavefish
Match: stk3 (serine/threonine kinase 3 [Source:NCBI gene;Acc:103027492]) HSP 1 Score: 93.9745 bits (232), Expect = 1.855e-24 Identity = 51/142 (35.92%), Postives = 77/142 (54.23%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ L S + F+K CL+ NP +R +A QL+ FI
Sbjct: 142 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFI 277 HSP 2 Score: 39.6614 bits (91), Expect = 1.855e-24 Identity = 36/126 (28.57%), Postives = 62/126 (49.21%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K S Q + +K+ +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E+E I L + LH ++ +HRDIK
Sbjct: 24 EVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQ-VPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILKSTLKGLEYLHFMRKIHRDIK 147
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Cavefish
Match: STK4 (serine/threonine kinase 4 [Source:HGNC Symbol;Acc:HGNC:11408]) HSP 1 Score: 85.1149 bits (209), Expect = 1.125e-22 Identity = 47/137 (34.31%), Postives = 70/137 (51.09%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLI 2929
I+ I A NI+L+ G AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ S F+ CL+ NP R +A QL+
Sbjct: 149 IHRDIKAGNILLNSEGQAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITAIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRNPDQWSEPFRDFVSQCLVKNPENRATATQLL 279 HSP 2 Score: 42.743 bits (99), Expect = 1.125e-22 Identity = 37/126 (29.37%), Postives = 62/126 (49.21%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQ-KCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K + E+V K +E L ++I I + + + Y K+ D + + M Y GS++ I+Q+ K + E+E I L + LH ++ +HRDIK
Sbjct: 31 EVFDVLEKLGEGSYGSVFKAHYK-ETGEIVAIKQVPVESDLQEIIKEISIMQQCNSPHVVRYYGSYFKNSD-LWIVMEYCGAGSVSDIIRQRNKTLAEDEIAAILQSTLKGLEYLHFMRKIHRDIK 154
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Cavefish
Match: map3k2 (mitogen-activated protein kinase kinase kinase 2 [Source:NCBI gene;Acc:103026704]) HSP 1 Score: 70.4774 bits (171), Expect = 1.156e-22 Identity = 47/146 (32.19%), Postives = 78/146 (53.42%), Query Frame = 2
Query: 2513 LYIYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELH--QFIKSCLIVNPNERPSAQQLIGSQFI 2944
+ ++ I NI+ D AGN KL DFG S+ ++ TI + +K+ GT +M+PE++ Y K DIWS G +VEM+T PP+ A A+ + K+ P++ + H F+K + V +RP+A++L+ F+
Sbjct: 481 MIVHRDIKGANILRDSAGNVKLGDFGASRRLQ--TICLSGTGIKSVT-GTPYWMSPEVISGEGYGRKADIWSVGCTVVEMLTLRPPW---AEFEAMAAIFKIATQPTNPALPAHVSDHCRDFLKR-IFVETKQRPAAEELLRHIFV 619 HSP 2 Score: 57.3806 bits (137), Expect = 1.156e-22 Identity = 41/138 (29.71%), Postives = 65/138 (47.10%), Query Frame = 3
Query: 2127 NFDFLKEFATGAFGKVFICQDKISSQELVLKK---------------STEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLA--MPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
N+ K GAFG+VF+C D + +EL +K+ + E E L +++F ++ QY ++D L+ M Y+ GGS+ +++ + EN K + QIL + LH IVHRDIK
Sbjct: 358 NWRLGKLLGQGAFGRVFLCYDADTGRELAVKQVQFDPDSPETSKEVSALECEIQLLKNLF-------HERIVQYYGCLRDTHERTLSIFMEYMPGGSIKDQLKSYGALTENVTRKYTRQILEGVCYLHSNMIVHRDIK 488
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Cavefish
Match: si:ch211-1i11.3 (mitogen-activated protein kinase kinase kinase 5-like [Source:NCBI gene;Acc:103038360]) HSP 1 Score: 76.6406 bits (187), Expect = 3.008e-22 Identity = 48/145 (33.10%), Postives = 76/145 (52.41%), Query Frame = 2
Query: 2531 ISADNIILDG-AGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSY--KIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSV--LISIELHQFIKSCLIVNPNERPSAQQLIGSQFIIG 2950
I DN++++ +G K+ DFG SK + + GT+ YMAPEI+ + P Y DIWS G ++EM TG PPFH+ +A + K+ +K P+V +S E + C NP++R +A +L+ + F+ G
Sbjct: 754 IKGDNVLINTYSGVLKISDFGTSKRLAGIN------PCTETFTGTLQYMAPEIIDQGPRGYGKPADIWSLGCTIIEMATGKPPFHELGSPQA--AMFKVGMFKIHPNVPESMSEEAKACLMCCFESNPDKRATASELLKNAFLRG 890 HSP 2 Score: 49.6766 bits (117), Expect = 3.008e-22 Identity = 42/119 (35.29%), Postives = 66/119 (55.46%), Query Frame = 3
Query: 2157 GAFGKVFICQD-----KISSQELVLKKSTEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQ-KCIHENEKIKISY--QILTQIHELHRLQIVHRDIK 2489
G +G V+ +D +I+ +E+ K ST + LH +I + RLK+ + + QY V + I++ M + GGSL+S ++ Q + +NE + Y QIL + LH QIVHRDIK
Sbjct: 639 GTYGVVYAGRDLSNQVRIAIKEIPEKDSTYSQ-PLHEEIALHKRLKHRN-IVQYLGSVSEGGFIKIFMEEVPGGSLSSLLRSQWGPLKDNEPTIVFYTKQILEGLKYLHDNQIVHRDIK 755
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Cavefish
Match: pak6b (serine/threonine-protein kinase PAK 6-like [Source:NCBI gene;Acc:103030407]) HSP 1 Score: 83.1889 bits (204), Expect = 7.094e-22 Identity = 49/143 (34.27%), Postives = 75/143 (52.45%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFII 2947
I+ I +D+I+L G KL DFG I + K K+ GT +MAPE++ + PY ++D+WS G+++VEMV G PP+ P A+ L+ + S IS L F+ S L +P +R SA L+ F++
Sbjct: 512 IHRDIKSDSILLTLDGRIKLSDFGFC-----AQISKDIPKRKSLV-GTPYWMAPEVISKTPYGTEVDVWSLGIMVVEMVDGEPPYFSDTPVAAMKRLRDEPAPSVRNSSKISPVLKDFLDSMLTRDPLQRSSASDLLKHPFLL 648 HSP 2 Score: 41.9726 bits (97), Expect = 7.094e-22 Identity = 37/131 (28.24%), Postives = 69/131 (52.67%), Query Frame = 3
Query: 2118 YMENFDFLKEFATGAFGKVFICQDKISSQELV-----LKKSTEMEFSLHRDIFI--GYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
Y+ENF + E G+ G V I ++K S +++ L+K E L ++ I YR KN ++++ + + +++ I M YL GG+LT+ + + + + E + + +L + LH ++HRDIK
Sbjct: 395 YLENFVKIGE---GSTGVVCIAREKHSGKQVAVKMMDLRKQQRREL-LFNEVVIMRDYRHKNVVEMYKSALVEEELWVI---MEYLQGGALTNIVSETR-LTEEQIATVCEAVLQALAYLHAQGVIHRDIK 517
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Sea Lamprey
Match: map3k2 (mitogen-activated protein kinase kinase kinase 2 [Source:ZFIN;Acc:ZDB-GENE-120215-96]) HSP 1 Score: 66.2402 bits (160), Expect = 4.811e-21 Identity = 43/143 (30.07%), Postives = 69/143 (48.25%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELH-QFIKSCLIVNPNERPSAQQLIGSQFI 2944
++ I NI+ D GN KL DFG SK ++ + + + GT +M+PE++ Y K DIWS G +VEM+T PP+ A A+ + K+ P + + H + + + V RPSA+ L+ F+
Sbjct: 483 VHRDIKGANILRDSTGNVKLGDFGASKRLQTICSQGTAMR---SVTGTPYWMSPEVINGEGYGRKADIWSVGCTVVEMLTEKPPW---AEFEAMAAIFKIATQPTDPRLPLHASEHARDLLRRIFVEAKLRPSAEDLLRHPFV 619 HSP 2 Score: 53.5286 bits (127), Expect = 4.811e-21 Identity = 42/134 (31.34%), Postives = 68/134 (50.75%), Query Frame = 3
Query: 2127 NFDFLKEFATGAFGKVFICQDKISSQELVLKK--------STEMEF-SLHRDIFIGYRLKN--SSKLFQYSRIVKDMD--SIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
N+ K GAFG+V++C D + +EL +K+ T E +L +I + LKN ++ QY ++D ++ + M Y+ GGS+ +++ + EN K + QIL + LH IVHRDIK
Sbjct: 358 NWQRGKLLGQGAFGRVYLCYDADTGRELAVKQVQFDPGSVETSKEVNALECEIQL---LKNLQHERIVQYFGCLRDPQQKTLSIFMEYMPGGSIKDQLRAYGALTENVTRKYTRQILEGVSFLHGNMIVHRDIK 488
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Sea Lamprey
Match: pak4 (p21 protein (Cdc42/Rac)-activated kinase 4 [Source:ZFIN;Acc:ZDB-GENE-040704-69]) HSP 1 Score: 80.1073 bits (196), Expect = 8.837e-20 Identity = 46/142 (32.39%), Postives = 76/142 (53.52%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I +D+I+L G KL DFG V+ E+ K GT +MAPE++ +PY ++D+WS G++++EMV G PP+ + P +A+ ++ V K K S L F+ L+ +P R +A +L+ F+
Sbjct: 535 IHRDIKSDSILLTADGRIKLSDFGFCA---QVSKEVTRRKSLV---GTPYWMAPEVISRLPYGPEVDVWSLGIMVIEMVDGEPPYFNEPPLQAMRRIRDNVPPKMKNIQKASPLLRGFLDQMLVRDPALRATALELLQHPFL 670 HSP 2 Score: 35.4242 bits (80), Expect = 8.837e-20 Identity = 31/131 (23.66%), Postives = 61/131 (46.56%), Query Frame = 3
Query: 2118 YMENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLHRDIFIG-------YRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
Y+ENF + E +TG V + D+ S +++ +KK + R++ Y +N ++ + D + + M +L GG+LT + + ++E + + +L + LH ++HRDIK
Sbjct: 418 YLENFIKIGEGSTGV---VCLAVDRRSGRQVAVKK-MHLRKQQRRELLFNEVVIMREYHHENVVDMYNSYLVA---DELWVVMEFLEGGALTDIVTHTR-MNEEQIATVCVSVLRALAYLHGQGVIHRDIK 540
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Sea Lamprey
Match: slka (STE20-like kinase a [Source:ZFIN;Acc:ZDB-GENE-050411-66]) HSP 1 Score: 84.7297 bits (208), Expect = 4.220e-19 Identity = 53/153 (34.64%), Postives = 79/153 (51.63%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEI-----LREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFIIGYLQN 2962
I+ + A NI+L G+ KL DFG+S +N L++ ++ GT +MAPE+ +++ PY YK DIWS G+ L+E+ PP H+ P R ++++ K S E F+K CL NP R +A QL+ FI G N
Sbjct: 151 IHRDLKAGNILLTFEGDVKLADFGVS--AKNTRT---LQRRDSFI-GTPYWMAPEVVMCETMKDAPYDYKADIWSLGITLIELAQMEPPNHELNPMRVMLKIAKNDPPSLSFPSKWSAEFTDFLKKCLDKNPETRWNALQLLQHSFISGVDSN 297 HSP 2 Score: 28.4906 bits (62), Expect = 4.220e-19 Identity = 29/130 (22.31%), Postives = 63/130 (48.46%), Query Frame = 3
Query: 2130 FDFLKEFATGAFGKVFICQDKIS---SQELVLKKSTEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDS------IQLAMPYLSGGSLTS-KIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
+ + E GAFGKV+ Q+K S + V++ +E E D + + L + IV +++ + + + + GG++ + ++ ++ + E + + Q+L ++ LH +I+HRD+K
Sbjct: 33 WSVIGELGDGAFGKVYKAQNKESGVLAAAKVIETKSEEELE---DYMVEIEI---LALCDHPNIVGLLNAFYYENKLWIFIEFCPGGAVDAIMLELERGLAEPQIRVVCKQMLEALNYLHTHRIIHRDLK 156
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006766.1 (pep scaffold:Pmarinus_7.0:GL478342:9725:27922:-1 gene:ENSPMAG00000006086.1 transcript:ENSPMAT00000006766.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 63.1586 bits (152), Expect = 4.874e-19 Identity = 38/102 (37.25%), Postives = 51/102 (50.00%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVE 2824
+Y + +NI+LD G+ L DFGL K I GT Y+APE+L++ PY +D W G VL EM+ G PPF+ K LK+M E
Sbjct: 213 VYRDLKPENILLDYQGHIVLTDFGLCKE------GIDPHSTTCTFCGTPEYLAPEVLKKHPYDRTVDWWCLGCVLYEMLYGLPPFYSK-------NLKQMYE 301 HSP 2 Score: 49.6766 bits (117), Expect = 4.874e-19 Identity = 38/134 (28.36%), Postives = 62/134 (46.27%), Query Frame = 3
Query: 2127 NFDFLKEFATGAFGKVFICQD---------KISSQELVLKKSTEMEFSLHRDIFIGYRLKNSSKLF----QYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
+FDFL G+FGKV + + K+ + ++L+K E R++ + KN F YS + D + M Y++GG L +Q+Q E+ + +I + I LH L IV+RD+K
Sbjct: 91 DFDFLTVLGKGSFGKVLLAKHKSDGIFYAVKVMKKSIILQKKEEKHIMAERNVLV----KNVRHPFLVGLHYS--FQSADKLYFVMDYVNGGELFFHLQRQGSFPESRAQFYAAEIGSAIGYLHSLNIVYRDLK 218
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008103.1 (pep scaffold:Pmarinus_7.0:GL476484:390774:392599:-1 gene:ENSPMAG00000007328.1 transcript:ENSPMAT00000008103.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 82.4185 bits (202), Expect = 1.175e-18 Identity = 42/124 (33.87%), Postives = 71/124 (57.26%), Query Frame = 2
Query: 2573 KLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
KL DFG V+ E+ K GT +MAPE++ +PY ++DIWS G++++EMV G PP+ + P +A+ ++ + K K S +++L F+ L+ +P +R SA +L+G F+
Sbjct: 3 KLSDFGFCA---QVSKEVTRRKSLV---GTPYWMAPEVISRLPYGPEVDIWSLGVMVMEMVDGEPPYFNEPPLQAMRRIRDNIPPKIKNSQGTTVQLRSFLDRMLVRDPTQRASAVELLGHPFL 120
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Yeast
Match: STE20 (Cdc42p-activated signal transducing kinase; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p; member of the PAK (p21-activated kinase) family [Source:SGD;Acc:S000000999]) HSP 1 Score: 88.5817 bits (218), Expect = 2.122e-21 Identity = 46/143 (32.17%), Postives = 77/143 (53.85%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFII 2947
++ I +DNI+L G+ KL DFG + + ++ + GT +MAPE++ Y K+DIWS G++++EM+ G PP+ + P RA+ + K K +S L +F+ CL V P +R SA +L+ ++I
Sbjct: 736 LHRDIKSDNILLSMEGDIKLTDFGFCAQINELNLK------RTTMVGTPYWMAPEVVSRKEYGPKVDIWSLGIMIIEMIEGEPPYLNETPLRALYLIATNGTPKLKEPENLSSSLKKFLDWCLCVEPEDRASATELLHDEYIT 872 HSP 2 Score: 31.5722 bits (70), Expect = 2.122e-21 Identity = 39/141 (27.66%), Postives = 62/141 (43.97%), Query Frame = 3
Query: 2088 N*FCSRGFASYMENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDS------IQLAMPYLSGGSLTSKIQQQKCI-HENEKIKISYQILTQIHELHRLQIVHRDIK 2489
N CS G S + L + GA G V+ + I + V K +E +++ I L K ++ IV +DS + + M Y+ GGSLT + CI E + + + L+ + LH ++HRDIK
Sbjct: 608 NEICSDGDPSTK--YANLVKIGQGASGGVYTAYE-IGTNVSVAIKQMNLEKQPKKELIINEIL--VMKGSKHPNIVNFIDSYVLKGDLWVIMEYMEGGSLTDVVTH--CILTEGQIGAVCRETLSGLEFLHSKGVLHRDIK 741
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Yeast
Match: YPL150W_mRNA (Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study [Source:SGD;Acc:S000006071]) HSP 1 Score: 79.337 bits (194), Expect = 7.517e-21 Identity = 51/147 (34.69%), Postives = 75/147 (51.02%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKS-MENVTIEILLEKLKAYAPGTVNYMAPEILREIPYS-YKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFIIGY 2953
++ + +NI+LD GNAKL DFG ++ M T+E + GT YMAPE++ Y +KIDIWS G++L ++TG PF A+ ++ E K+ V I + I L NP ERPS Q++ F+ Y
Sbjct: 154 VHRDLKLENILLDKNGNAKLTDFGFTRECMTKTTLETVC--------GTTVYMAPELIERRTYDGFKIDIWSLGVILYTLITGYLPFDDDDEAKTKWKIVNE-EPKYDAKV-IPDDARDLISRLLAKNPGERPSLSQVLRHPFLQPY 290 HSP 2 Score: 39.2762 bits (90), Expect = 7.517e-21 Identity = 29/123 (23.58%), Postives = 62/123 (50.41%), Query Frame = 3
Query: 2127 NFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLHRDIFI--GYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
N+ LK+ G+FGKV++ + + +++ LK S + + ++ R++F + +KL++ ++ + +A+ Y G L + + I E ++ QI ++ H + VHRD+K
Sbjct: 40 NYKILKQIGEGSFGKVYLALHRPTHRKVCLKTSDKNDPNIVREVFYHRQFDFPYITKLYE---VIVTESKVWMALEYCPGKELYDHLLSLRRISLLECGELFAQISGAVYYAHSMHCVHRDLK 159
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Yeast
Match: SKM1 (Member of the PAK family of serine/threonine protein kinases; similar to Ste20p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth; SKM1 has a paralog, CLA4, that arose from the whole genome duplication [Source:SGD;Acc:S000005473]) HSP 1 Score: 86.6557 bits (213), Expect = 6.595e-20 Identity = 47/143 (32.87%), Postives = 80/143 (55.94%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLK-AYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I +DNI+L+ G K+ DFG +E+ ++ K A GT +MAPEI+ + Y K+D+WS G++L+EM+ G PP+ + P +A+ + K + +S + QF+ +CL VN R S ++L+ +F+
Sbjct: 504 IHRDIKSDNILLNSQGLVKITDFGF-------CVELTEKRSKRATMVGTPYWMAPEIVNQKGYDEKVDVWSLGIMLIEMIEGEPPYLNEDPLKALYLIANNGSPKLRHPESVSKQTKQFLDACLQVNVESRASVRKLLTFEFL 639 HSP 2 Score: 28.8758 bits (63), Expect = 6.595e-20 Identity = 18/66 (27.27%), Postives = 31/66 (46.97%), Query Frame = 3
Query: 2319 DMDSIQLAMPYLSGGSLTSKI---------QQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
D + + + M Y+ GG LT + + ++EN+ I + + LH +I+HRDIK
Sbjct: 444 DDEELWVIMEYMEGGCLTDILDAVARSNTGEHSSPLNENQMAYIVKETCQGLKFLHNKKIIHRDIK 509
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Yeast
Match: CLA4 (Cdc42p-activated signal transducing kinase; member of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a paralog, SKM1, that arose from the whole genome duplication [Source:SGD;Acc:S000005242]) HSP 1 Score: 82.8037 bits (203), Expect = 1.952e-19 Identity = 43/142 (30.28%), Postives = 76/142 (53.52%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I +DN++LD K+ DFG + + + +A GT +MAPE++++ Y KID+WS G++ +EM+ G PP+ + P +A+ + K K +S+E+ +F+ CL V+ R S ++L+ F
Sbjct: 690 IHRDIKSDNVLLDTRARVKITDFGFCARLTDKRSK------RATMVGTPYWMAPEVVKQREYDEKIDVWSLGIMTIEMLEGEPPYLNEDPLKALYLIATNGTPKLKHPESLSLEIKRFLSVCLCVDVRYRASTEELLHHGFF 825 HSP 2 Score: 30.8018 bits (68), Expect = 1.952e-19 Identity = 28/109 (25.69%), Postives = 48/109 (44.04%), Query Frame = 3
Query: 2187 DKISSQELVLKKSTEMEFSLHRDIFI-GYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHE-------LHRLQIVHRDIK 2489
DK++ +++VL K E ++ + + R KN + ++ D + + M ++ GGSLT I+ + QI + E LH I+HRDIK
Sbjct: 589 DKVAIKQMVLSKQPRKELIVNEILVMKDSRHKNIVNFLEA--YLRTDDDLWVVMEFMEGGSLTDIIENSPTNDNSHSPLTEPQIAYIVRETCQGLKFLHDKHIIHRDIK 695
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Yeast
Match: MKK2 (MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication [Source:SGD;Acc:S000006061]) HSP 1 Score: 76.2554 bits (186), Expect = 6.859e-19 Identity = 52/156 (33.33%), Postives = 76/156 (48.72%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAI--IELKKMV-----EYKFKPSVLISIE--LHQFIKSCLIVNPNERPSAQQLIGSQFIIGYLQ 2959
I+ I NI+L+ G KL DFG+S N L GT YMAPE ++ PYS D+WS G+ L+E+ G PF + + IEL M+ + K +P + IS FI CL + ERPS +Q++ +I+G ++
Sbjct: 339 IHRDIKPQNILLNEKGEIKLCDFGVSGEAVN--------SLAMTFTGTSFYMAPERIQGQPYSVTCDVWSLGLTLLEVAGGRFPFESDKITQNVAPIELLTMILTFSPQLKDEPELDISWSKTFRSFIDYCLKKDARERPSPRQMLKHPWIVGQMK 486 HSP 2 Score: 35.4242 bits (80), Expect = 6.859e-19 Identity = 37/127 (29.13%), Postives = 58/127 (45.67%), Query Frame = 3
Query: 2148 FATGAFGKVFICQDKISSQELVLKK----STEMEFSLHRDIFIGYRLKNSSK---LFQYSRIVKD--MDSIQLAMPYLSGGSLTSK----IQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
GA G V C+ K + LK +T+ E+ + IF + S K + QY + D SI +AM Y+ G SL + +++ I E KI+ +L + LH +++HRDIK
Sbjct: 220 LGEGAGGSVAKCRLKNGKKVFALKTINTMNTDPEY--QKQIFRELQFNKSFKSDYIVQYYGMFTDEQSSSIYIAMEYMGGKSLEATYKNLLKRGGRISERVIGKIAESVLRGLSYLHERKVIHRDIK 344
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Nematostella
Match: EDO41410 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4G7]) HSP 1 Score: 81.2629 bits (199), Expect = 1.574e-28 Identity = 47/138 (34.06%), Postives = 72/138 (52.17%), Query Frame = 2
Query: 2531 ISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I NI+ D GN KL DFG SK ++ + + + GT +MAPE++ Y K D+WS G +VEM+T PP+ P A+ ++ PS L S++ +F++S L +N RPSA +L+ F+
Sbjct: 130 IKGANILRDLYGNVKLADFGASKRLQTIRSKTGFRSVH----GTPYWMAPEVINGEGYGRKADVWSVGCTVVEMLTTKPPWADFEPMAALFKIATQPTEPELPSDL-SVDAVEFVRSTLRMNSKSRPSADELLRFSFV 262 HSP 2 Score: 64.6994 bits (156), Expect = 1.574e-28 Identity = 48/132 (36.36%), Postives = 69/132 (52.27%), Query Frame = 3
Query: 2121 MENFDFLKEFATGAFGKVFICQDKISSQELVLKK--------STEMEF-SLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
M N+ K GAFG+V++C D + +EL +K+ ST+ E +L +I +N ++ QY I D I + M YL GGS+ I+Q ++E+ K S QIL I LH +IVHRDIK
Sbjct: 1 MNNWRKGKLLGAGAFGQVYMCHDLDTGRELAVKQIETGQLNSSTKNEVKALEGEIEFMKAFRNE-RIVQYYGIETDDLHIYIFMEYLPGGSIHEHIKQHGALNESLTRKYSRQILEGILYLHTNRIVHRDIK 131
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Nematostella
Match: EDO42845 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0A9]) HSP 1 Score: 92.0485 bits (227), Expect = 3.244e-23 Identity = 50/147 (34.01%), Postives = 76/147 (51.70%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFIIGYLQ 2959
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++E+ Y DIWS G+ +EM G PP+ P RAI + F+ S + F+ CL+ NP ER +A +L+ FI G Q
Sbjct: 140 IHRDIKAGNILLNSEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEVGYDCLADIWSLGITAMEMAEGKPPYADIHPMRAIFMIPTKPPPTFRDPGQWSEDFKDFVSKCLVKNPEERATATELLQHSFIQGAKQ 280 HSP 2 Score: 36.1946 bits (82), Expect = 3.244e-23 Identity = 34/127 (26.77%), Postives = 62/127 (48.82%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKK---STEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTS--KIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K S Q + +K+ T+++ + ++I + + + + Y K+ D + + M Y GS++ KI+ + E K + Y L + LH + +HRDIK
Sbjct: 22 EVFDVLEKLGEGSYGSVFKAMHKESGQVVAIKQVPVDTDLQ-EIIKEISMMQQCDSPYVVKYYGSYFKNTD-LWIVMEYCGAGSVSDLMKIRNKTLTEEEIKCVLKY-TLKGLEYLHLRRKIHRDIK 145
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Nematostella
Match: EDO48741 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RIM4]) HSP 1 Score: 86.2705 bits (212), Expect = 1.204e-22 Identity = 51/153 (33.33%), Postives = 80/153 (52.29%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEIL-----REIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFIIGYLQN 2962
I+ + A N++L GN K+ DFG+S + L+K + GT +MAPE++ ++ PY YK DIWS G+ L+E+ PP H P R + ++ K F S +L+ F+ SC++ NP+ R SA ++I FI + N
Sbjct: 140 IHRDLKAGNLLLASDGNVKMADFGVSAKNKKT-----LQKRSTFI-GTPYWMAPEVVVTETYKDDPYDYKADIWSAGITLIELAQMVPPNHNMHPMRVLFKIPKSDPPTFDNPNKWSNDLNDFLASCVVKNPDMRASATEMIKHPFIADFTDN 286 HSP 2 Score: 39.6614 bits (91), Expect = 1.204e-22 Identity = 33/128 (25.78%), Postives = 66/128 (51.56%), Query Frame = 3
Query: 2127 NFDFLKEFATGAFGKVFICQDKISS-----QELVLKKSTEME-FSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTS-KIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
N++ + E G+FGKV+ Q K S + +++ E+E F + DI + KN LF+ + D + + + + SGG+L ++ ++ ++E E ++ Q+ + LH +++HRD+K
Sbjct: 21 NWELVGELGDGSFGKVYKAQHKTESSLSAAKIIIVNDENELEDFMVEIDILSECKHKNLVGLFET--FLHD-GKLWMMLEFCSGGALDDLMLELERGLNEPEIRAVTRQLFEGLQFLHNHKVIHRDLK 145
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Nematostella
Match: EDO41242 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4Z6]) HSP 1 Score: 60.077 bits (144), Expect = 2.907e-20 Identity = 38/125 (30.40%), Postives = 69/125 (55.20%), Query Frame = 3
Query: 2127 NFDFLKEFATGAFGKVFICQDKISSQELVLKK----STEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
N+D L+ G+F KVF C ++ + E K+ E + + ++I + L++ + + YS I ++ +++ L M Y++GGSL ++ QQ E + ++ Q+L + LH +IVHRDIK
Sbjct: 4 NYDMLELIGEGSFAKVFRCIERKTGLEFAAKELRLTDVEDKEKIEQEIAVWKDLRHENIVSLYSSI-REGETVCLIMEYVAGGSLFDEVIQQTYYSEKQARLVTRQLLNALEYLHSRRIVHRDIK 127 HSP 2 Score: 57.7658 bits (138), Expect = 2.907e-20 Identity = 38/143 (26.57%), Postives = 73/143 (51.05%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNA---KLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVL---ISIELHQFIKSCLIVNPNERPSAQQLI 2929
++ I DN++L GN KL DFGL++++ N T + + A G ++APE + E P +DIW+ G++ ++ G PPF + + ++ + + +Y PS +S + + ++V+P R SA++ +
Sbjct: 122 VHRDIKPDNLLLKRTGNNVTIKLADFGLAQALPNDT-----DVISCGASGAPMFLAPETVLEEPIGRAVDIWACGVIFYLLLVGYPPFWSNSDEQLLLSILRG-QYTM-PSPFWDNVSNDARDLVHKLIVVDPEARISAKEAL 257
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Nematostella
Match: EDO45846 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRL1]) HSP 1 Score: 82.4185 bits (202), Expect = 7.694e-20 Identity = 48/142 (33.80%), Postives = 78/142 (54.93%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I +D+I+L G KL DFG VT ++ K GT +MAPE++ PY ++DIWS G++++EMV G PP+ + P +A+ +L+ + + + IS L F+ + LI +P R +A +L+ FI
Sbjct: 145 IHRDIKSDSILLTSNGTVKLSDFGFCA---QVTEDMPRRKSLV---GTPYWMAPEVISRKPYGTEVDIWSLGIMVLEMVDGEPPYFNEPPLQAMRKLRDLEPPTSRNPIQISPRLQSFLDNALIRDPLYRATAFELLNHPFI 280 HSP 2 Score: 33.8834 bits (76), Expect = 7.694e-20 Identity = 30/129 (23.26%), Postives = 59/129 (45.74%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLHRDIFIG-------YRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
EN + G+ G V + DK + +++ +KK +++ R++ Y N +++ S +V D + + M +L GG+LT + + E + + +L + LH ++HRDIK
Sbjct: 27 ENLGNFMKIGEGSTGIVCLATDKRTGRQVAVKK-MDLKKQQRRELLFNEVVIMRDYPHPNIVEMYG-SHLVGD--ELWVVMEFLEGGTLTDIVTHTN-LSEEQVACVCRAVLKALTFLHSQGVIHRDIK 150
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Medaka
Match: stk3 (serine/threonine-protein kinase 3 [Source:NCBI gene;Acc:101155570]) HSP 1 Score: 93.9745 bits (232), Expect = 2.504e-24 Identity = 51/142 (35.92%), Postives = 77/142 (54.23%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ L S + F+K CL+ NP +R +A QL+ FI
Sbjct: 143 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFI 278 HSP 2 Score: 39.6614 bits (91), Expect = 2.504e-24 Identity = 36/126 (28.57%), Postives = 62/126 (49.21%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K S Q + +K+ +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E+E I L + LH ++ +HRDIK
Sbjct: 25 EVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQ-VPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILKSTLKGLEYLHFMRKIHRDIK 148
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Medaka
Match: stk3 (serine/threonine-protein kinase 3 [Source:NCBI gene;Acc:101155570]) HSP 1 Score: 93.9745 bits (232), Expect = 2.588e-24 Identity = 51/142 (35.92%), Postives = 77/142 (54.23%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G+AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ L S + F+K CL+ NP +R +A QL+ FI
Sbjct: 146 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFI 281 HSP 2 Score: 39.2762 bits (90), Expect = 2.588e-24 Identity = 36/126 (28.57%), Postives = 62/126 (49.21%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K S Q + +K+ +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E+E I L + LH ++ +HRDIK
Sbjct: 28 EVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQ-VPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTD-LWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILKSTLKGLEYLHFMRKIHRDIK 151
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Medaka
Match: ENSORLT00000044704.1 (serine/threonine-protein kinase 4 [Source:NCBI gene;Acc:101175466]) HSP 1 Score: 90.8929 bits (224), Expect = 1.457e-23 Identity = 50/142 (35.21%), Postives = 73/142 (51.41%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ L S F+ CL+ NP R +A QL+ FI
Sbjct: 149 IHRDIKAGNILLNAEGQAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITAIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRNPDLWSENFRDFVSQCLVKNPENRATATQLLQHPFI 284 HSP 2 Score: 39.6614 bits (91), Expect = 1.457e-23 Identity = 35/126 (27.78%), Postives = 61/126 (48.41%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K + E+V K +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E E + + L + LH ++ +HRDIK
Sbjct: 31 EVFDVLEKLGEGSYGCVFKANYK-ETGEIVAIKQVPVESDLQEIIKEISIMQQCNSPHVVRYYGSYFKNSD-LWIVMEYCGAGSVSDIIRIRNKTLSEEEIATVVHSTLKGLEYLHFMRKIHRDIK 154
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Medaka
Match: ENSORLT00000024938.2 (serine/threonine-protein kinase 4 [Source:NCBI gene;Acc:101175466]) HSP 1 Score: 90.8929 bits (224), Expect = 2.251e-23 Identity = 50/142 (35.21%), Postives = 73/142 (51.41%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFI 2944
I+ I A NI+L+ G AKL DFG++ + + + + GT +MAPE+++EI Y+ DIWS G+ +EM G PP+ P RAI + F+ L S F+ CL+ NP R +A QL+ FI
Sbjct: 149 IHRDIKAGNILLNAEGQAKLADFGVAGQLTDTMAK------RNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITAIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRNPDLWSENFRDFVSQCLVKNPENRATATQLLQHPFI 284 HSP 2 Score: 39.2762 bits (90), Expect = 2.251e-23 Identity = 35/126 (27.78%), Postives = 61/126 (48.41%), Query Frame = 3
Query: 2124 ENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLH---RDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQ-QQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
E FD L++ G++G VF K + E+V K +E L ++I I + + + Y K+ D + + M Y GS++ I+ + K + E E + + L + LH ++ +HRDIK
Sbjct: 31 EVFDVLEKLGEGSYGCVFKANYK-ETGEIVAIKQVPVESDLQEIIKEISIMQQCNSPHVVRYYGSYFKNSD-LWIVMEYCGAGSVSDIIRIRNKTLSEEEIATVVHSTLKGLEYLHFMRKIHRDIK 154
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Medaka
Match: map3k3 (mitogen-activated protein kinase kinase kinase 3 [Source:NCBI gene;Acc:101169290]) HSP 1 Score: 75.8702 bits (185), Expect = 7.746e-22 Identity = 48/141 (34.04%), Postives = 73/141 (51.77%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQF 2941
++ I NI+ D AGN KL DFG SK ++ TI + +++ GT +M+PE++ Y K D+WS G +VEM+T PP+ + AI ++ PS S + FI C+ V RPSA++L+ F
Sbjct: 477 VHRDIKGANILRDSAGNVKLGDFGASKRLQ--TICMSGTGIRSVT-GTPYWMSPEVISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPLLPS-HTSDQARDFIH-CIFVEAKHRPSAEELLRHPF 612 HSP 2 Score: 49.2914 bits (116), Expect = 7.746e-22 Identity = 42/129 (32.56%), Postives = 64/129 (49.61%), Query Frame = 3
Query: 2142 KEFATGAFGKVFICQDKISSQELVLKK--------STEMEFS-LHRDIFIGYRLKN--SSKLFQYSRIVKDMD--SIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
K GAFG V++C D + +EL K+ T E S L +I + LKN ++ QY ++D ++ + M Y+ GGS+ +++ + EN K + QIL + LH IVHRDIK
Sbjct: 357 KLLGQGAFGLVYLCYDVDTGRELAAKQVQFDPDSPETSKEVSALECEIQL---LKNLHHERIVQYYGCLRDHSEKTLTIFMEYMPGGSVKDQLKAYGALTENVTRKYTRQILEGMSYLHSNMIVHRDIK 482
BLAST of Non-specific serine/threonine protein kinase vs. Planmine SMEST
Match: SMESG000024565.1 (SMESG000024565.1) HSP 1 Score: 915.605 bits (2365), Expect = 0.000e+0 Identity = 443/443 (100.00%), Postives = 443/443 (100.00%), Query Frame = 2
Query: 185 MKIFVIRVENPEIKIEIKLEEYSLVRNIRNKLDKIFPGRVKWNKHFIKLVHKNIIMDDEKSIGWYKLCEGDEILCVRIENYLCSDDVILEVFLSGFVPEVIYLIVQRNYSISKIKSMIERIQCEIINICDFTGNKMTGSQTVTETSSCAYYRLIVKYGRKETCISTFEDCLKQYHKFRIIHYYDFILRLRHLYECLFETRINEVIKQMNEIKAIMLSNDPFEIRLKENLSEFPSAFIKCHSIFCDDISSRIWDICDDYWQLSRNVIENDEWIVIGLFFRMALLIVLHQYLEHERNHCEIATKTFNSIVVDALGYSVTLLIKIRQMESFCETAGYSFDLIGELMEDICVKWMDPFLSTSRYYSEFDEVFNRYYNKTFASLKFGNGSVEFHFHSDPQTIIEAKEAMAQKCGVKVNDLYAIYQRNEDKPTDGEPYDETILDFVLMI 1513
MKIFVIRVENPEIKIEIKLEEYSLVRNIRNKLDKIFPGRVKWNKHFIKLVHKNIIMDDEKSIGWYKLCEGDEILCVRIENYLCSDDVILEVFLSGFVPEVIYLIVQRNYSISKIKSMIERIQCEIINICDFTGNKMTGSQTVTETSSCAYYRLIVKYGRKETCISTFEDCLKQYHKFRIIHYYDFILRLRHLYECLFETRINEVIKQMNEIKAIMLSNDPFEIRLKENLSEFPSAFIKCHSIFCDDISSRIWDICDDYWQLSRNVIENDEWIVIGLFFRMALLIVLHQYLEHERNHCEIATKTFNSIVVDALGYSVTLLIKIRQMESFCETAGYSFDLIGELMEDICVKWMDPFLSTSRYYSEFDEVFNRYYNKTFASLKFGNGSVEFHFHSDPQTIIEAKEAMAQKCGVKVNDLYAIYQRNEDKPTDGEPYDETILDFVLMI
Sbjct: 1 MKIFVIRVENPEIKIEIKLEEYSLVRNIRNKLDKIFPGRVKWNKHFIKLVHKNIIMDDEKSIGWYKLCEGDEILCVRIENYLCSDDVILEVFLSGFVPEVIYLIVQRNYSISKIKSMIERIQCEIINICDFTGNKMTGSQTVTETSSCAYYRLIVKYGRKETCISTFEDCLKQYHKFRIIHYYDFILRLRHLYECLFETRINEVIKQMNEIKAIMLSNDPFEIRLKENLSEFPSAFIKCHSIFCDDISSRIWDICDDYWQLSRNVIENDEWIVIGLFFRMALLIVLHQYLEHERNHCEIATKTFNSIVVDALGYSVTLLIKIRQMESFCETAGYSFDLIGELMEDICVKWMDPFLSTSRYYSEFDEVFNRYYNKTFASLKFGNGSVEFHFHSDPQTIIEAKEAMAQKCGVKVNDLYAIYQRNEDKPTDGEPYDETILDFVLMI 443 HSP 2 Score: 262.692 bits (670), Expect = 2.222e-138 Identity = 127/142 (89.44%), Postives = 133/142 (93.66%), Query Frame = 2
Query: 2531 ISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFIIGYL 2956
I DNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAP R +IELKKMVEY+FKP++LISIE +QFIK CL V+PNERPSAQQLI SQFII YL
Sbjct: 566 IKPDNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPPRVVIELKKMVEYEFKPNLLISIEFNQFIKWCLTVDPNERPSAQQLIESQFIIDYL 707 HSP 3 Score: 251.136 bits (640), Expect = 2.222e-138 Identity = 123/123 (100.00%), Postives = 123/123 (100.00%), Query Frame = 3
Query: 2121 MENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
MENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK
Sbjct: 445 MENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 567
BLAST of Non-specific serine/threonine protein kinase vs. Planmine SMEST
Match: SMESG000024565.1 (SMESG000024565.1) HSP 1 Score: 261.922 bits (668), Expect = 7.503e-139 Identity = 127/142 (89.44%), Postives = 133/142 (93.66%), Query Frame = 2
Query: 2531 ISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFIIGYL 2956
I DNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAP R +IELKKMVEY+FKP++LISIE +QFIK CL V+PNERPSAQQLI SQFII YL
Sbjct: 122 IKPDNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPPRVVIELKKMVEYEFKPNLLISIEFNQFIKWCLTVDPNERPSAQQLIESQFIIDYL 263 HSP 2 Score: 253.447 bits (646), Expect = 7.503e-139 Identity = 123/123 (100.00%), Postives = 123/123 (100.00%), Query Frame = 3
Query: 2121 MENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
MENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK
Sbjct: 1 MENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 123
BLAST of Non-specific serine/threonine protein kinase vs. Planmine SMEST
Match: SMESG000024532.1 (SMESG000024532.1) HSP 1 Score: 276.174 bits (705), Expect = 1.705e-105 Identity = 134/148 (90.54%), Postives = 139/148 (93.92%), Query Frame = 2
Query: 2519 IYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFIIGYLQN 2962
I+ I DNI+ DGAGNAKLIDFGLSK M+N T E +LEKLKAYAPGT+NYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFIIGYLQN
Sbjct: 539 IHRDIKPDNIVFDGAGNAKLIDFGLSKWMKNSTDENILEKLKAYAPGTINYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFIIGYLQN 686 HSP 2 Score: 128.257 bits (321), Expect = 1.705e-105 Identity = 63/123 (51.22%), Postives = 87/123 (70.73%), Query Frame = 3
Query: 2121 MENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
MEN + KEF G FG+VF QD IS ++L +K + + SL+RDIFI + N+S+LF+YS + K + +AMPY++GGSLTS IQ +K + E E ++ISYQIL QI +LH L+I+HRDIK
Sbjct: 422 MENMNLGKEFGAGGFGRVFFYQDIISGKQLAMKTANSDKSSLYRDIFISALIMNNSELFKYSGLFKYKSELYIAMPYMTGGSLTSYIQGKKGLFEKEALRISYQILIQIEKLHELKIIHRDIK 544 HSP 3 Score: 251.136 bits (640), Expect = 3.627e-71 Identity = 162/437 (37.07%), Postives = 244/437 (55.84%), Query Frame = 2
Query: 185 MKIFVIRVENPEIKIEIKLEEYSLVRNIRNKLDKIFPGRVKWNKHFIKLVHKNIIMDDEKSIGWYKLCEGDEILCVRIENYLCSDDVILEVFLSGFVPEVIYLIVQRNYSISKIKSMIERIQCEIINICDFTGNKMTGSQTVTETSSCAYYRLIVKYGRKETCISTFEDCLKQYHKFRIIHYYDFILRLRHLYECLFETRINEVIKQMNEIKAIMLSNDPFEIRLKENLSEFPSAFIKCHSIFCDDISSRIWDICDDYWQLSRNVIENDEWIVIGLFFRMALLIVLHQYLEHERNHCEIATKTFNSIVVDALGYSVTLLIKIRQMESFCETAGYSFDLIGELMEDICVKWMDPFLSTSRYYSEFDEVFNRYYNKTFASL---KFGNGSVEFHFHSDPQTIIEAKEAMAQKCGVKVNDLYAIYQRNEDKPTDGEPYDE 1486
MKI V+ EN +K I+++++S V +I++K+DKI G+ KW K ++ ++KNII+ DE I YK+CE DEI +IENYL SDDVI+EVFL+G P +I+LIV+ N+SI+ I+SMIE +Q ++INI D+ G +M Q V++ YYRLIV Y + +FEDC++Q+ +F + F L +E + T IN V +N+I+ IM ND + RLKENLS PS + +F +I RI DIC ++W+ +++ + +I F +++L+ LHQY + E K+FN IV+DAL Y V + + C+ Y L + D FL R SE+ E+ N Y ++ + K N + T+++AK +AQK GV + DLY I N DK + + Y E
Sbjct: 1 MKISVVNGENLSVKFVIEIDKHSYVGDIKHKIDKILNGQGKWEKEHLRFIYKNIILHDE-YIKRYKVCESDEIWFGKIENYLRSDDVIIEVFLAGCSPVLIWLIVKINHSITVIRSMIEIVQSKLINIYDWNGREMQNVQIVSDIDPIGYYRLIVSYEDERGKWLSFEDCVRQHDEFT----FRFCLNSEFEFEDM--TEINTV-NLINKIRTIMSLNDVIDARLKENLSNIPSGVVNSKLLFYYNIQLRILDICQEFWKSTKDSPYYINYNIIKS-FELSILVALHQYSHSRQKDPENKDKSFNGIVIDALDYLV------KHLHDHCKDLKYHQKL-----------FFDHFLLVPRPVSEYIELRNEYSSQLITKIIINKKDNIAPFLTLLLTQGTVLDAKCEIAQKLGVSIKDLYCINHWNHDKAEEDKIYKE 411
BLAST of Non-specific serine/threonine protein kinase vs. Planmine SMEST
Match: SMESG000024843.1 (SMESG000024843.1) HSP 1 Score: 160.229 bits (404), Expect = 4.745e-74 Identity = 78/143 (54.55%), Postives = 101/143 (70.63%), Query Frame = 2
Query: 2531 ISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFIIGYLQ 2959
I +NI+ D N KLIDFGL+K + + + L K KA +PGT+NYMAPE+ +E P S KIDIWSFGMV+VEMVTG PF+ P + ++ L K+++Y+FKP + IS +L QFI CL V+PN RP+AQQLI SQ I YLQ
Sbjct: 416 IKPENIVFDNERNVKLIDFGLAKYIGDFRSKDLSRKRKASSPGTINYMAPELTKEDPNSVKIDIWSFGMVMVEMVTGQLPFNVMGPGQILLRLGKLLKYEFKPMMEISNDLKQFISWCLTVDPNVRPTAQQLIESQLINNYLQ 558 HSP 2 Score: 139.043 bits (349), Expect = 4.745e-74 Identity = 69/125 (55.20%), Postives = 94/125 (75.20%), Query Frame = 3
Query: 2115 SYMENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
S M +F+ L E A G FGKVFIC+DKIS +++V+KK +++ SLHR+I IG L + +L +YS +++D +I L MPYL GG+LTS IQQ KC+ ENE I+IS+QILTQI ELH+ +I+H DIK
Sbjct: 294 SKMADFENLNELANGGFGKVFICEDKISGKQVVMKKLIDVKDSLHREILIGSILFDK-ELIKYSEVIRDEKNIHLTMPYLPGGTLTSNIQQLKCLGENEVIRISHQILTQISELHKSKIIHCDIK 417 HSP 3 Score: 160.999 bits (406), Expect = 3.439e-41 Identity = 104/317 (32.81%), Postives = 167/317 (52.68%), Query Frame = 2
Query: 590 MTGSQTVTETSSCAYYRLIVKYGRKETCISTFEDCLKQYHKFRIIHYYDFILRLRHLYECLFETRINEVIKQMNEIKAIMLSNDPFEIRLKENLSEFPSAFIKCHSIFCDDISSRIWDICDDYWQL------SRNVIENDEWIVIGLFFRMALLIVLHQYLEHERNHCEIATKTFNSIVVDALGYSVTLLIKIRQMESFCETAGYSFDLIGELMEDICVKWMDPFLSTSRYYSEFDEVFNRYYNKTFASLKFGNGSVEFHFHSDPQTIIEAKEAMAQKCGVKVNDLYAIYQRNEDKPTDGEPYDETILDFVLMIKFV 1522
M +Q+VT+ + YYRLIV G++ TF++C Q+ + +II YY +E E ++I Q+ +I+ I+ SND + RL ENLS FP AF+K + +F DI+ RIWDIC++Y +L S NDEW + LFF ALL+ + +Y + +N + K+FN I+VDAL V R + + CE ++ +W++ F ++ + DE+F + ++ + ++ + E +P+TI+EA +A+A K + DLY+I DKP DG+PY + + F LM K
Sbjct: 1 MQNNQSVTDIDAFGYYRLIVNCGQEADDNLTFDECCNQHDRCKIILYY--YSENWWEFEKFSEIARVKIINQIKKIRQIISSNDTIDPRLNENLSHFPKAFVKQNYLFGHDITKRIWDICNEYRKLMKSYPFSVQFCLNDEWHAMKLFFLTALLVTMRKYSDAVQNDPKNTKKSFNDILVDALDCLV------RHLMNPCEDWKHA-----------AKRWLNSFFLITQSDGDMDELFVK-FDPSMIIVRIKSEEYEMDVELNPKTILEATKAVANKFRIDRQDLYSINHWIPDKPNDGDPYFQPMTFFELMSKMA 297
BLAST of Non-specific serine/threonine protein kinase vs. Planmine SMEST
Match: SMESG000024843.1 (SMESG000024843.1) HSP 1 Score: 160.229 bits (404), Expect = 4.824e-74 Identity = 78/143 (54.55%), Postives = 101/143 (70.63%), Query Frame = 2
Query: 2531 ISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPGTVNYMAPEILREIPYSYKIDIWSFGMVLVEMVTGNPPFHKKAPARAIIELKKMVEYKFKPSVLISIELHQFIKSCLIVNPNERPSAQQLIGSQFIIGYLQ 2959
I +NI+ D N KLIDFGL+K + + + L K KA +PGT+NYMAPE+ +E P S KIDIWSFGMV+VEMVTG PF+ P + ++ L K+++Y+FKP + IS +L QFI CL V+PN RP+AQQLI SQ I YLQ
Sbjct: 424 IKPENIVFDNERNVKLIDFGLAKYIGDFRSKDLSRKRKASSPGTINYMAPELTKEDPNSVKIDIWSFGMVMVEMVTGQLPFNVMGPGQILLRLGKLLKYEFKPMMEISNDLKQFISWCLTVDPNVRPTAQQLIESQLINNYLQ 566 HSP 2 Score: 139.043 bits (349), Expect = 4.824e-74 Identity = 69/125 (55.20%), Postives = 94/125 (75.20%), Query Frame = 3
Query: 2115 SYMENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLHRDIFIGYRLKNSSKLFQYSRIVKDMDSIQLAMPYLSGGSLTSKIQQQKCIHENEKIKISYQILTQIHELHRLQIVHRDIK 2489
S M +F+ L E A G FGKVFIC+DKIS +++V+KK +++ SLHR+I IG L + +L +YS +++D +I L MPYL GG+LTS IQQ KC+ ENE I+IS+QILTQI ELH+ +I+H DIK
Sbjct: 302 SKMADFENLNELANGGFGKVFICEDKISGKQVVMKKLIDVKDSLHREILIGSILFDK-ELIKYSEVIRDEKNIHLTMPYLPGGTLTSNIQQLKCLGENEVIRISHQILTQISELHKSKIIHCDIK 425 HSP 3 Score: 166.777 bits (421), Expect = 4.730e-43 Identity = 107/323 (33.13%), Postives = 171/323 (52.94%), Query Frame = 2
Query: 572 DFTGNKMTGSQTVTETSSCAYYRLIVKYGRKETCISTFEDCLKQYHKFRIIHYYDFILRLRHLYECLFETRINEVIKQMNEIKAIMLSNDPFEIRLKENLSEFPSAFIKCHSIFCDDISSRIWDICDDYWQL------SRNVIENDEWIVIGLFFRMALLIVLHQYLEHERNHCEIATKTFNSIVVDALGYSVTLLIKIRQMESFCETAGYSFDLIGELMEDICVKWMDPFLSTSRYYSEFDEVFNRYYNKTFASLKFGNGSVEFHFHSDPQTIIEAKEAMAQKCGVKVNDLYAIYQRNEDKPTDGEPYDETILDFVLMIKFV 1522
D GN+M +Q+VT+ + YYRLIV G++ TF++C Q+ + +II YY +E E ++I Q+ +I+ I+ SND + RL ENLS FP AF+K + +F DI+ RIWDIC++Y +L S NDEW + LFF ALL+ + +Y + +N + K+FN I+VDAL V R + + CE ++ +W++ F ++ + DE+F + ++ + ++ + E +P+TI+EA +A+A K + DLY+I DKP DG+PY + + F LM K
Sbjct: 3 DCKGNEMQNNQSVTDIDAFGYYRLIVNCGQEADDNLTFDECCNQHDRCKIILYY--YSENWWEFEKFSEIARVKIINQIKKIRQIISSNDTIDPRLNENLSHFPKAFVKQNYLFGHDITKRIWDICNEYRKLMKSYPFSVQFCLNDEWHAMKLFFLTALLVTMRKYSDAVQNDPKNTKKSFNDILVDALDCLV------RHLMNPCEDWKHA-----------AKRWLNSFFLITQSDGDMDELFVK-FDPSMIIVRIKSEEYEMDVELNPKTILEATKAVANKFRIDRQDLYSINHWIPDKPNDGDPYFQPMTFFELMSKMA 305 The following BLAST results are available for this feature:
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30027773 ID=SMED30027773|Name=Non-specific serine/threonine protein kinase|organism=Schmidtea mediterranea sexual|type=transcript|length=3128bpback to top protein sequence of SMED30027773-orf-1 >SMED30027773-orf-1 ID=SMED30027773-orf-1|Name=SMED30027773-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=153bp MLLYIYFYISADNIILDGAGNAKLIDFGLSKSMENVTIEILLEKLKAYAPback to top protein sequence of SMED30027773-orf-2 >SMED30027773-orf-2 ID=SMED30027773-orf-2|Name=SMED30027773-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=447bp MKIFVIRVENPEIKIEIKLEEYSLVRNIRNKLDKIFPGRVKWNKHFIKLVback to top protein sequence of SMED30027773-orf-3 >SMED30027773-orf-3 ID=SMED30027773-orf-3|Name=SMED30027773-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=145bp MENFDFLKEFATGAFGKVFICQDKISSQELVLKKSTEMEFSLHRDIFIGYback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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