TNF receptor-associated factor

Overview
NameTNF receptor-associated factor
Smed IDSMED30027755
Length (bp)1607
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of TNF receptor-associated factor (SMED30027755) t-SNE clustered cells

Violin plots show distribution of expression levels for TNF receptor-associated factor (SMED30027755) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of TNF receptor-associated factor (SMED30027755) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for TNF receptor-associated factor (SMED30027755) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30027755

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30027755h1SMcG0019375 SmedASXL_011055SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30027755h1SMcG0019375 SmedASXL_011055SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Human
Match: TRAF3 (TNF receptor associated factor 3 [Source:HGNC Symbol;Acc:HGNC:12033])

HSP 1 Score: 88.1965 bits (217), Expect = 6.963e-18
Identity = 47/146 (32.19%), Postives = 76/146 (52.05%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLV 1549
            WKI +   +  +A  G    ++S+ FY+   GYK+C ++Y  G     G  LS++F I +GEYD  LPWP  Q+++L L+DQ S      D +      +   + F +PT ++N   G   FV   V+ N   I  +++I I+ +V
Sbjct:  337 WKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFK----PDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIK-DDTIFIKVIV 477
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Human
Match: TRAF3 (TNF receptor associated factor 3 [Source:HGNC Symbol;Acc:HGNC:12033])

HSP 1 Score: 87.8113 bits (216), Expect = 9.825e-18
Identity = 47/146 (32.19%), Postives = 76/146 (52.05%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLV 1549
            WKI +   +  +A  G    ++S+ FY+   GYK+C ++Y  G     G  LS++F I +GEYD  LPWP  Q+++L L+DQ S      D +      +   + F +PT ++N   G   FV   V+ N   I  +++I I+ +V
Sbjct:  395 WKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFK----PDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIK-DDTIFIKVIV 535
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Human
Match: TRAF3 (TNF receptor associated factor 3 [Source:HGNC Symbol;Acc:HGNC:12033])

HSP 1 Score: 87.8113 bits (216), Expect = 9.825e-18
Identity = 47/146 (32.19%), Postives = 76/146 (52.05%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLV 1549
            WKI +   +  +A  G    ++S+ FY+   GYK+C ++Y  G     G  LS++F I +GEYD  LPWP  Q+++L L+DQ S      D +      +   + F +PT ++N   G   FV   V+ N   I  +++I I+ +V
Sbjct:  395 WKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFK----PDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIK-DDTIFIKVIV 535
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Human
Match: TRAF3 (TNF receptor associated factor 3 [Source:HGNC Symbol;Acc:HGNC:12033])

HSP 1 Score: 87.8113 bits (216), Expect = 1.159e-17
Identity = 47/146 (32.19%), Postives = 76/146 (52.05%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLV 1549
            WKI +   +  +A  G    ++S+ FY+   GYK+C ++Y  G     G  LS++F I +GEYD  LPWP  Q+++L L+DQ S      D +      +   + F +PT ++N   G   FV   V+ N   I  +++I I+ +V
Sbjct:  420 WKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFK----PDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIK-DDTIFIKVIV 560
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Human
Match: TRAF3 (TNF receptor associated factor 3 [Source:HGNC Symbol;Acc:HGNC:12033])

HSP 1 Score: 87.8113 bits (216), Expect = 1.159e-17
Identity = 47/146 (32.19%), Postives = 76/146 (52.05%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLV 1549
            WKI +   +  +A  G    ++S+ FY+   GYK+C ++Y  G     G  LS++F I +GEYD  LPWP  Q+++L L+DQ S      D +      +   + F +PT ++N   G   FV   V+ N   I  +++I I+ +V
Sbjct:  420 WKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFK----PDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIK-DDTIFIKVIV 560
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Celegans
Match: trf-1 (TNF Receptor Associated Factor (TRAF) homolog; TRF-1 [Source:UniProtKB/TrEMBL;Acc:G5EG80])

HSP 1 Score: 69.707 bits (169), Expect = 1.699e-12
Identity = 41/138 (29.71%), Postives = 70/138 (50.72%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKI--CLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQ------KSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFN 1501
            WKI +L ++  +A+ G ++ IFS  F SH  GYK+  C  L+  G   ++G+ +S+Y  + KGE+D  L WP  + I + L+DQ      + +  +  D         A +   ++P  + N+  GS  F  L ++ N
Sbjct:  267 WKIDKLQQRTNEAKSGADTTIFSVPFMSHRFGYKMMACACLF--GDGSSAGKSISLYVLLLKGEFDPTLEWPFHRAIKISLLDQNPRPEDRVNITYVIDPRKL----KANEKFLARPRGERNAAFGSQSFCSLAILQN 398
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Celegans
Match: trf-2 (TNF Receptor Associated Factor (TRAF) homolog [Source:UniProtKB/TrEMBL;Acc:Q9N586])

HSP 1 Score: 54.6842 bits (130), Expect = 7.471e-8
Identity = 26/81 (32.10%), Postives = 45/81 (55.56%), Query Frame = 2 
Query: 1112 WKISELSEKVTKA-REGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLI 1351
            ++I ++   V KA +  ++  I+S+ FYSH  GYK+     P G   A   + S++ C+ KGE+DD L WP    ++  ++
Sbjct:  162 FRIPKIGLTVVKATKNKSHRSIYSQPFYSHGYGYKMMAVAAPYGDGLAFREYFSVFVCLMKGEWDDILEWPFRCDVTFSIL 242
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Fly
Match: Traf4 (gene:FBgn0026319 transcript:FBtr0334401)

HSP 1 Score: 59.6918 bits (143), Expect = 3.269e-9
Identity = 41/162 (25.31%), Postives = 75/162 (46.30%), Query Frame = 2 
Query: 1058 LNSVESIVSNNYRYAPFEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINS-GLGSSEFVKLNVIFNNKRILFNNSIVIR 1540
            L S  S +S NY      WKI++ S K+ +AR  +   + S  FY+   GYK+   ++  G  P     +S+Y  +  GEYD  L WP    I+  L +Q + +       S+      E   F +P+ + +  G G   F+  + + +++  +  +++ +R
Sbjct:  309 LKSAVSKLSINY-TGTLLWKITDWSAKMAEARGKDGLELVSPPFYTSQYGYKLQASMFLNGNGPGENTHVSVYIKVLPGEYDALLKWPFSHSITFTLFEQGAQSGQGGVAESFVPDPTWEN--FQRPSNEPDQLGFGFPRFIS-HELLHSRPFIKGDTVFLR 466
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Fly
Match: Traf4 (gene:FBgn0026319 transcript:FBtr0077429)

HSP 1 Score: 58.9214 bits (141), Expect = 5.972e-9
Identity = 41/162 (25.31%), Postives = 75/162 (46.30%), Query Frame = 2 
Query: 1058 LNSVESIVSNNYRYAPFEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINS-GLGSSEFVKLNVIFNNKRILFNNSIVIR 1540
            L S  S +S NY      WKI++ S K+ +AR  +   + S  FY+   GYK+   ++  G  P     +S+Y  +  GEYD  L WP    I+  L +Q + +       S+      E   F +P+ + +  G G   F+  + + +++  +  +++ +R
Sbjct:  318 LKSAVSKLSINY-TGTLLWKITDWSAKMAEARGKDGLELVSPPFYTSQYGYKLQASMFLNGNGPGENTHVSVYIKVLPGEYDALLKWPFSHSITFTLFEQGAQSGQGGVAESFVPDPTWEN--FQRPSNEPDQLGFGFPRFIS-HELLHSRPFIKGDTVFLR 475
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Fly
Match: Traf6 (gene:FBgn0265464 transcript:FBtr0071188)

HSP 1 Score: 57.3806 bits (137), Expect = 2.062e-8
Identity = 37/150 (24.67%), Postives = 62/150 (41.33%), Query Frame = 2 
Query: 1052 FELNSVESIVSNNYRYAPFEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINS-GLGSSEFVKLNVIF 1498
              L   ++ V   Y      W+I +L   V + R   N+ ++S   Y+ P GYK C +L    + P     LS++  + + E D  L WP   +I L ++     T+   D          E   F +P   I++ G G  E+  ++ I 
Sbjct:  314 LRLARQQAPVDPRYSNGTIVWRIEQLGALVARLRANANNQVYSHECYTSPHGYKFCARL---NIQPRKPHVLSLHVHLMQSENDYHLDWPFKGRIKLCMVHPADATLSQHDTIM----TKPEILAFHKPREAISTRGFGFLEYANISNII 456
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Zebrafish
Match: traf2b (Tnf receptor-associated factor 2b [Source:ZFIN;Acc:ZDB-GENE-030131-5345])

HSP 1 Score: 90.5077 bits (223), Expect = 7.787e-19
Identity = 50/154 (32.47%), Postives = 76/154 (49.35%), Query Frame = 2 
Query: 1106 FEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSD----TVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI 1555
            F WKI+E S +   A  G    +FS +FYS   GYK+CL+LY  G     G  LS++F + KG+YD  L WP  Q+++L L+DQ +       F  D  S           F +P +++N   G   F  L  +      L +++I I+ +V +
Sbjct:  385 FIWKIAEFSRRRQDAVAGRAPAMFSPAFYSSKYGYKMCLRLYLNGDGTGRGSHLSLFFVVMKGKYDALLKWPFSQKVTLMLLDQNNREHIIDAFRPDVSS---------TSFQRPISEMNIASGCPLFCPLAKLAGKSSYLRDDTIFIKAIVDL 529
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Zebrafish
Match: traf2b (Tnf receptor-associated factor 2b [Source:ZFIN;Acc:ZDB-GENE-030131-5345])

HSP 1 Score: 90.5077 bits (223), Expect = 7.787e-19
Identity = 50/154 (32.47%), Postives = 76/154 (49.35%), Query Frame = 2 
Query: 1106 FEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSD----TVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI 1555
            F WKI+E S +   A  G    +FS +FYS   GYK+CL+LY  G     G  LS++F + KG+YD  L WP  Q+++L L+DQ +       F  D  S           F +P +++N   G   F  L  +      L +++I I+ +V +
Sbjct:  385 FIWKIAEFSRRRQDAVAGRAPAMFSPAFYSSKYGYKMCLRLYLNGDGTGRGSHLSLFFVVMKGKYDALLKWPFSQKVTLMLLDQNNREHIIDAFRPDVSS---------TSFQRPISEMNIASGCPLFCPLAKLAGKSSYLRDDTIFIKAIVDL 529
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Zebrafish
Match: traf2b (Tnf receptor-associated factor 2b [Source:ZFIN;Acc:ZDB-GENE-030131-5345])

HSP 1 Score: 90.1225 bits (222), Expect = 1.090e-18
Identity = 50/154 (32.47%), Postives = 76/154 (49.35%), Query Frame = 2 
Query: 1106 FEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSD----TVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI 1555
            F WKI+E S +   A  G    +FS +FYS   GYK+CL+LY  G     G  LS++F + KG+YD  L WP  Q+++L L+DQ +       F  D  S           F +P +++N   G   F  L  +      L +++I I+ +V +
Sbjct:  398 FIWKIAEFSRRRQDAVAGRAPAMFSPAFYSSKYGYKMCLRLYLNGDGTGRGSHLSLFFVVMKGKYDALLKWPFSQKVTLMLLDQNNREHIIDAFRPDVSS---------TSFQRPISEMNIASGCPLFCPLAKLAGKSSYLRDDTIFIKAIVDL 542
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Zebrafish
Match: BX901915.1 (pep chromosome:GRCz11:CHR_ALT_CTG5_1_18:28764891:28768979:-1 gene:ENSDARG00000109857.1 transcript:ENSDART00000191629.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX901915.1)

HSP 1 Score: 85.1149 bits (209), Expect = 5.360e-18
Identity = 47/154 (30.52%), Postives = 78/154 (50.65%), Query Frame = 2 
Query: 1106 FEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSD----TVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI 1555
            F WKIS+ S+K   A  G    +FS +FY+   GYK+CL++Y  G     G  LS++F + +G  D  L WP  Q+++L L+DQ +       F  D  S         + F +P +D+N   G   F  L+ + +    + +++I I+ +V +
Sbjct:  128 FVWKISDFSKKRQDAVAGRAPAMFSPAFYTSKYGYKMCLRIYLNGDGTGRGTHLSLFFVVMRGHSDALLKWPFNQKVTLMLLDQNNREHIIDAFRPDISS---------SSFQRPVSDMNIASGCPLFCPLSKLDSKNSYIRDDTIFIKAIVDL 272
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Zebrafish
Match: AL844899.1 (pep chromosome:GRCz11:CHR_ALT_CTG5_2_4:28756846:28760787:-1 gene:ENSDARG00000111038.1 transcript:ENSDART00000183806.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:AL844899.1)

HSP 1 Score: 85.1149 bits (209), Expect = 5.360e-18
Identity = 47/154 (30.52%), Postives = 78/154 (50.65%), Query Frame = 2 
Query: 1106 FEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSD----TVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI 1555
            F WKIS+ S+K   A  G    +FS +FY+   GYK+CL++Y  G     G  LS++F + +G  D  L WP  Q+++L L+DQ +       F  D  S         + F +P +D+N   G   F  L+ + +    + +++I I+ +V +
Sbjct:  128 FVWKISDFSKKRQDAVAGRAPAMFSPAFYTSKYGYKMCLRIYLNGDGTGRGTHLSLFFVVMRGHSDALLKWPFNQKVTLMLLDQNNREHIIDAFRPDISS---------SSFQRPVSDMNIASGCPLFCPLSKLDSKNSYIRDDTIFIKAIVDL 272
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Xenopus
Match: hspb6 (heat shock protein family B (small) member 6 [Source:Xenbase;Acc:XB-GENE-876273])

HSP 1 Score: 91.2781 bits (225), Expect = 2.366e-19
Identity = 48/150 (32.00%), Postives = 78/150 (52.00%), Query Frame = 2 
Query: 1106 FEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKS-DTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFN-NSIVIRCLV 1549
            F WKIS+L+ K   A  G    ++S +FY+   GYK+CL++Y  G     G  +S++F + KGEYD  LPWP   +++  L+D  + D+VF +         +   A F +P  D+N   G   F  +  + + K      N++ IRC++
Sbjct:  232 FLWKISDLTNKSHDAVTGRAISLYSPAFYTAKYGYKVCLRIYLNGDGAGKGTHVSLFFTVMKGEYDALLPWPFKHKVTFVLVDHNNRDSVFDAFR------PDVTSASFQRPINDMNVASGCPLFCPIAKLNSPKHAYIRENTLYIRCII 375
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Xenopus
Match: ssh2 (slingshot protein phosphatase 2 [Source:Xenbase;Acc:XB-GENE-993093])

HSP 1 Score: 88.5817 bits (218), Expect = 5.498e-18
Identity = 77/263 (29.28%), Postives = 125/263 (47.53%), Query Frame = 2 
Query:  806 EENDQKNQFIIRKLQQSIEEIKSKAARKSKEIE-VRLTQLEEYYLQS--ETKQEKLEQ-KVFALXXXXXXXXXXXXHKTKHDLATCFELNSVESI--VSNNYRYAPFE---------WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLV 1549
            +E  + N+  I +LQ+ IE    K     KEI  +R T  E   ++S  E+ Q +L Q +V A ++   NP   ++   +HD     ++ SV  I     + R+   E         WKI +   +  +A  G    ++S+ FY+   GYK+C ++Y  G     G  LS++F I +GEYD  LPWP  Q++SL L+DQ        D +      +   + F +PT ++N   G   FV   V+ N   I  +++I I+ +V
Sbjct:  301 KEQLRNNEARIIQLQRVIEGQADKLKDLDKEIRNLRQTGDEADGMKSNYESLQNRLSQLEVMAKNAASWNPGVLETQINRHD-----QMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVSLMLMDQGPSRRHLGDAFK----PDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIK-DDTIFIKVIV 553
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Xenopus
Match: ssh2 (slingshot protein phosphatase 2 [Source:Xenbase;Acc:XB-GENE-993093])

HSP 1 Score: 88.1965 bits (217), Expect = 6.110e-18
Identity = 77/263 (29.28%), Postives = 125/263 (47.53%), Query Frame = 2 
Query:  806 EENDQKNQFIIRKLQQSIEEIKSKAARKSKEIE-VRLTQLEEYYLQS--ETKQEKLEQ-KVFALXXXXXXXXXXXXHKTKHDLATCFELNSVESI--VSNNYRYAPFE---------WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLV 1549
            +E  + N+  I +LQ+ IE    K     KEI  +R T  E   ++S  E+ Q +L Q +V A ++   NP   ++   +HD     ++ SV  I     + R+   E         WKI +   +  +A  G    ++S+ FY+   GYK+C ++Y  G     G  LS++F I +GEYD  LPWP  Q++SL L+DQ        D +      +   + F +PT ++N   G   FV   V+ N   I  +++I I+ +V
Sbjct:  276 KEQLRNNEARIIQLQRVIEGQADKLKDLDKEIRNLRQTGDEADGMKSNYESLQNRLSQLEVMAKNAASWNPGVLETQINRHD-----QMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVSLMLMDQGPSRRHLGDAFK----PDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIK-DDTIFIKVIV 528
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Xenopus
Match: slc12a1 (solute carrier family 12 member 1 [Source:Xenbase;Acc:XB-GENE-479925])

HSP 1 Score: 85.8853 bits (211), Expect = 4.392e-17
Identity = 45/150 (30.00%), Postives = 77/150 (51.33%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILF--NNSIVIRCLVGI 1555
            WKI++   K  +A EG    IFS+ FY+   GY++C + Y  G     G FLS+YF + KGE+D  L WP  Q+++L L+DQ       +D +      +   + F +P +++N   G   F     + N K   +  ++++ I+ +V +
Sbjct:  388 WKITDYERKKREAMEGRAVSIFSQHFYTSWCGYRLCARAYLNGDGSGKGSFLSLYFVVMKGEFDSILLWPFKQKVTLMLLDQSGKKNHITDVFR----ADPNSSSFKRPDSEMNIASGCPRFASHAQLENPKNGCYIKDDTLFIKIVVDL 533
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Xenopus
Match: slc12a1 (solute carrier family 12 member 1 [Source:Xenbase;Acc:XB-GENE-479925])

HSP 1 Score: 85.1149 bits (209), Expect = 6.300e-17
Identity = 41/123 (33.33%), Postives = 64/123 (52.03%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFV 1480
            WKI++   K  +A EG    IFS+ FY+   GY++C + Y  G     G FLS+YF + KGE+D  L WP  Q+++L L+DQ       +D +      +   + F +P +++N   G   F 
Sbjct:  387 WKITDYERKKREAMEGRAVSIFSQHFYTSWCGYRLCARAYLNGDGSGKGSFLSLYFVVMKGEFDSILLWPFKQKVTLMLLDQSGKKNHITDVFR----ADPNSSSFKRPDSEMNIASGCPRFA 505
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BLAST of TNF receptor-associated factor vs. Ensembl Mouse
Match: Traf3 (TNF receptor-associated factor 3 [Source:MGI Symbol;Acc:MGI:108041])

HSP 1 Score: 87.8113 bits (216), Expect = 6.786e-18
Identity = 47/146 (32.19%), Postives = 76/146 (52.05%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLV 1549
            WKI +   +  +A  G    ++S+ FY+   GYK+C ++Y  G     G  LS++F I +GEYD  LPWP  Q+++L L+DQ S      D +      +   + F +PT ++N   G   FV   V+ N   I  +++I I+ +V
Sbjct:  394 WKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFK----PDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYI-KDDTIFIKVIV 534
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Mouse
Match: Traf3 (TNF receptor-associated factor 3 [Source:MGI Symbol;Acc:MGI:108041])

HSP 1 Score: 87.8113 bits (216), Expect = 6.786e-18
Identity = 47/146 (32.19%), Postives = 76/146 (52.05%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLV 1549
            WKI +   +  +A  G    ++S+ FY+   GYK+C ++Y  G     G  LS++F I +GEYD  LPWP  Q+++L L+DQ S      D +      +   + F +PT ++N   G   FV   V+ N   I  +++I I+ +V
Sbjct:  394 WKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFK----PDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYI-KDDTIFIKVIV 534
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Mouse
Match: Traf3 (TNF receptor-associated factor 3 [Source:MGI Symbol;Acc:MGI:108041])

HSP 1 Score: 87.8113 bits (216), Expect = 7.799e-18
Identity = 47/146 (32.19%), Postives = 76/146 (52.05%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLV 1549
            WKI +   +  +A  G    ++S+ FY+   GYK+C ++Y  G     G  LS++F I +GEYD  LPWP  Q+++L L+DQ S      D +      +   + F +PT ++N   G   FV   V+ N   I  +++I I+ +V
Sbjct:  419 WKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFK----PDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIK-DDTIFIKVIV 559
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Mouse
Match: Traf5 (TNF receptor-associated factor 5 [Source:MGI Symbol;Acc:MGI:107548])

HSP 1 Score: 81.6481 bits (200), Expect = 5.524e-17
Identity = 42/137 (30.66%), Postives = 69/137 (50.36%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDT-----VFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNK 1507
            WK+++   K  +A EG+   +FS+ FY+   GY++C + Y  G     G  LS+YF + +GE+D  L WP  Q+++L L+DQ          F +D  S         + F +P  ++N   G   FV  + + N+K
Sbjct:  100 WKVTDYRVKKREAVEGHTVSVFSQPFYTSRCGYRLCARAYLNGDGSGKGTHLSLYFVVMRGEFDSLLQWPFRQRVTLMLLDQSGKKNHIVETFKADPNS---------SSFKRPDGEMNIASGCPRFVSHSTLENSK 227
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Mouse
Match: Traf5 (TNF receptor-associated factor 5 [Source:MGI Symbol;Acc:MGI:107548])

HSP 1 Score: 81.6481 bits (200), Expect = 5.524e-17
Identity = 42/137 (30.66%), Postives = 69/137 (50.36%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDT-----VFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNK 1507
            WK+++   K  +A EG+   +FS+ FY+   GY++C + Y  G     G  LS+YF + +GE+D  L WP  Q+++L L+DQ          F +D  S         + F +P  ++N   G   FV  + + N+K
Sbjct:  100 WKVTDYRVKKREAVEGHTVSVFSQPFYTSRCGYRLCARAYLNGDGSGKGTHLSLYFVVMRGEFDSLLQWPFRQRVTLMLLDQSGKKNHIVETFKADPNS---------SSFKRPDGEMNIASGCPRFVSHSTLENSK 227
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BLAST of TNF receptor-associated factor vs. UniProt/SwissProt
Match: sp|Q60803|TRAF3_MOUSE (TNF receptor-associated factor 3 OS=Mus musculus OX=10090 GN=Traf3 PE=1 SV=2)

HSP 1 Score: 87.8113 bits (216), Expect = 5.455e-17
Identity = 47/146 (32.19%), Postives = 76/146 (52.05%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLV 1549
            WKI +   +  +A  G    ++S+ FY+   GYK+C ++Y  G     G  LS++F I +GEYD  LPWP  Q+++L L+DQ S      D +      +   + F +PT ++N   G   FV   V+ N   I  +++I I+ +V
Sbjct:  419 WKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFK----PDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIK-DDTIFIKVIV 559
[Close]
BLAST of TNF receptor-associated factor vs. UniProt/SwissProt
Match: sp|Q13114|TRAF3_HUMAN (TNF receptor-associated factor 3 OS=Homo sapiens OX=9606 GN=TRAF3 PE=1 SV=2)

HSP 1 Score: 87.8113 bits (216), Expect = 5.567e-17
Identity = 47/146 (32.19%), Postives = 76/146 (52.05%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLV 1549
            WKI +   +  +A  G    ++S+ FY+   GYK+C ++Y  G     G  LS++F I +GEYD  LPWP  Q+++L L+DQ S      D +      +   + F +PT ++N   G   FV   V+ N   I  +++I I+ +V
Sbjct:  420 WKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFK----PDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIK-DDTIFIKVIV 560
[Close]
BLAST of TNF receptor-associated factor vs. UniProt/SwissProt
Match: sp|O00463|TRAF5_HUMAN (TNF receptor-associated factor 5 OS=Homo sapiens OX=9606 GN=TRAF5 PE=1 SV=2)

HSP 1 Score: 84.7297 bits (208), Expect = 5.229e-16
Identity = 44/136 (32.35%), Postives = 69/136 (50.74%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDT----VFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNK 1507
            WK+++   K  +A +G+   IFS+SFY+   GY++C + Y  G     G  LS+YF + +GE+D  L WP  Q+++L L+DQ         F  D  S         + F +P  ++N   G   FV  +V+ N K
Sbjct:  408 WKVTDYKMKKREAVDGHTVSIFSQSFYTSRCGYRLCARAYLNGDGSGRGSHLSLYFVVMRGEFDSLLQWPFRQRVTLMLLDQSGKKNIMETFKPDPNS---------SSFKRPDGEMNIASGCPRFVAHSVLENAK 534
[Close]
BLAST of TNF receptor-associated factor vs. UniProt/SwissProt
Match: sp|Q12933|TRAF2_HUMAN (TNF receptor-associated factor 2 OS=Homo sapiens OX=9606 GN=TRAF2 PE=1 SV=2)

HSP 1 Score: 82.8037 bits (203), Expect = 1.903e-15
Identity = 70/245 (28.57%), Postives = 117/245 (47.76%), Query Frame = 2 
Query:  842 KLQQSIEEIKSKAARKSKEIEVRLTQLEEYYLQSE--TKQEKLEQ-KVFALXXXXXXXXXXXXHKTKHDLATCFELNSVESIVSNNYRYAPFEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSD----TVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI 1555
            +L Q  E ++ K A     + V   ++E   + +E  ++Q +L+Q K+ ALSS+    + S   K   DLA       V  + ++ Y    F WKIS+ + K  +A  G    IFS +FY+   GYK+CL++Y  G     G  LS++F + KG  D  L WP  Q+++L L+DQ +       F  D  S         + F +P  D+N   G   F  ++ +      + +++I I+ +V +
Sbjct:  267 ELLQRCESLEKKTATFENIVCVLNREVERVAMTAEACSRQHRLDQDKIEALSSKVQQLERSIGLK---DLAMADLEQKVLEMEASTYDGV-FIWKISDFARKRQEAVAGRIPAIFSPAFYTSRYGYKMCLRIYLNGDGTGRGTHLSLFFVVMKGPNDALLRWPFNQKVTLMLLDQNNREHVIDAFRPDVTS---------SSFQRPVNDMNIASGCPLFCPVSKMEAKNSYVRDDAIFIKAIVDL 498
[Close]
BLAST of TNF receptor-associated factor vs. UniProt/SwissProt
Match: sp|P39429|TRAF2_MOUSE (TNF receptor-associated factor 2 OS=Mus musculus OX=10090 GN=Traf2 PE=1 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.080e-15
Identity = 46/154 (29.87%), Postives = 76/154 (49.35%), Query Frame = 2 
Query: 1106 FEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSD----TVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI 1555
            F WKIS+ + K  +A  G    IFS +FY+   GYK+CL++Y  G     G  LS++F + KG  D  L WP  Q+++L L+D  +       F  D  S         + F +P +D+N   G   F  ++ +      + +++I I+ +V +
Sbjct:  354 FIWKISDFTRKRQEAVAGRTPAIFSPAFYTSRYGYKMCLRVYLNGDGTGRGTHLSLFFVVMKGPNDALLQWPFNQKVTLMLLDHNNREHVIDAFRPDVTS---------SSFQRPVSDMNIASGCPLFCPVSKMEAKNSYVRDDAIFIKAIVDL 498
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BLAST of TNF receptor-associated factor vs. TrEMBL
Match: T1E101 (TRAF-6 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 1.022e-26
Identity = 125/489 (25.56%), Postives = 217/489 (44.38%), Query Frame = 2 
Query:  149 IVGLKYEGVWKLLRKYHF-VIEIAKYEEFICIICRGLLKNAHQNKCGCRYCYECIIDFIHSDEKHCPGPTEECSQGLIDNNSDLQIDHTANRTVANILVPCPISPCGVVIELKYMVHHVKLHKCHSKSSNFYDADFG--------DETASETCPS--SHVNLLTDRIGFLNEKVXXXXXXXXXXXXKVINIQSLVEEIKWKQDSAIENLKVINDKTTEKLRLARETLLSTEENDQ--KNQFI--IRKLQQSIEEIKSKAARKSKEIEVRLTQLEEYYLQSETKQEK-LEQKVFALXXXXXXXXXXXXHKTKHDLATCFELNSVESIVSNNYRYAPFEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKS-----DTVFSSDEYSYYSCNNAEKAVFSQPTTDINS-GLGSSEFVKLNVIFNNKRILFNNSIVIRCLV 1549
            ++  K + V +LL KY+   ++ +   + +CI+C  L+++A ++ CGC YCY CI  ++  +  +CPG   EC +  ++   D+  +   +  ++ I+V CP   C    EL  +  H  L KC  +S        G        DE A         H  L+   + F NE   ++    + ++E + +++ ++   K   D     ++ +     E+LR     +L   ++ Q  KN+ I  +   +  ++++KS      +EIE     LE         Q K L++++F +     +    +  K    +     LN +   V        F W+I E S+K     +     ++S+SF SH   YK CL LY  G +      L ++  I KGE DDFL WPM + ++L +I+Q S      TVF  D Y         K  F +P  + N   +G+   V+ + I     IL N+SI++ C+V
Sbjct:    1 MIPSKLDNVQELLSKYNVKRVDESVNNDLLCILCNKLMRDACESACGCHYCYTCIRIYLSQNHNYCPGDKTECKEDYLNLEGDVFRNIRLSTRISRIVVKCPNIRCNYTSELLRLKEH--LDKCTPESVECPCVTIGCEEEPLLKDEMAKHLTDQVLRHTQLI---MSFCNELKSEINHNNETTKEDISHLKEVLRVQKALNDEKTAQMEFL----IEELRNKDNMILVLSQDIQEVKNEMITLLTSKESELKKLKSDIQIFRQEIEFMKNDLEVVGSTDIKNQVKDLKKEIFGIKMNIDSNNEERERK----MNNLIRLNKLCPNVD-----MDFMWRIEEYSKKRRTVTD-----VYSDSFQSH--NYKFCLNLYIYGDN------LGLWCVIMKGEKDDFLEWPMRRTVTLSIIEQDSLKTHKTTVFQYDTYQ------RNKKAFDRPKDERNDYCIGTDSLVRYSYIKETPMILVNDSIIVACVV 452
[Close]
BLAST of TNF receptor-associated factor vs. TrEMBL
Match: D5JG59 (TNF receptor-associated factor-2-like protein A OS=Dugesia japonica OX=6161 GN=TRAF-2A PE=2 SV=1)

HSP 1 Score: 110.923 bits (276), Expect = 6.007e-23
Identity = 58/177 (32.77%), Postives = 97/177 (54.80%), Query Frame = 2 
Query: 1025 KTKHDLATCFELNSVESIVSNN--YRYAPFEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLV 1549
            KT  +L       S+++I + N  +R A F WK ++   +   A  G    + S++FY+   GYK+CL++YP G     G  LS++F I KGE+DD L WP  Q+++L ++DQ+S +   SD +      +A  A + +PT + N   G  +F+ L++I NN     N++I ++  V
Sbjct:  176 KTIEELKKKAPATSIQTIDNKNVIFRNASFIWKFTDFEVRRVDAESGGRVSVTSDAFYTSEFGYKMCLRVYPAGDGVGKGTHLSVFFAIMKGEFDDLLSWPFKQKVTLSILDQESGSKPHSDTFK----PDARSACYQKPTDECNIASGFPKFILLSLIMNNSIYCKNDTIFLKIAV 348
[Close]
BLAST of TNF receptor-associated factor vs. TrEMBL
Match: A0A0F7GY08 (TNF receptor associated factor 5 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 1.238e-22
Identity = 55/141 (39.01%), Postives = 80/141 (56.74%), Query Frame = 2 
Query: 1100 APFEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTT----DINSGLGSSEFVKLNVIFNNKR 1510
            APFEWKI+   EK  +A  G    ++SE FYSH  GYKI L++ P G +     +LS+ F I  GEYDD L WPM +Q+ + LI+QK+  V  S  Y + S     K +F +P +    D +   G+ +    +V+FNN++
Sbjct:   46 APFEWKITNFMEKHERAISGEKLTVYSEPFYSHKNGYKIQLRIDPNGDEAVKALYLSVSFGIIGGEYDDELDWPMTKQVKISLINQKTGLVHISKRYKFDSVLKKSKCIFFKPISRFEDDEDYFFGTLKLSNFSVLFNNQK 186
[Close]
BLAST of TNF receptor-associated factor vs. TrEMBL
Match: A0A1S3K6C1 (TNF receptor-associated factor 2 OS=Lingula unguis OX=7574 GN=LOC106179037 PE=4 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 1.345e-21
Identity = 113/438 (25.80%), Postives = 196/438 (44.75%), Query Frame = 2 
Query:  224 EEFICIICRGLLKNAHQNKCGCRYCYECIIDFIHSDEKHCPGPTEECSQGLIDNNSDLQI--DHTANRTVANILVPCPISPCGVVIELK-YMVHHVKLHKCHSKSSNFYDADFGD---ETASETCPSSHVNLLTDRIGFLNEKVXXXXXXXXXXXXKVIN-IQSLVEEIKWKQDSAIENLKVINDK--------TTEKLRLARETLLSTEENDQKNQFIIRKLQQSIEEIKSKAARKSKEIEVRLTQLEEYYLQSETKQEKLEQKVFALXXXXXXXXXXXXHKTKHDLATCFELNSVESIVSNNYRYAPFEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDT----VFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFV 1480
            E F C  C+ +L++A Q  CG R+C  C+        + CP    +C +  ID   D QI  D    R V  +LV C  + C    +LK Y  +H +   C S+     + D  D   +  +E    + ++ +  R+  L ++V   QQ   E+  + +  ++  ++E + +     E +  IN +        +T ++   R+ L + E      + ++  + + IE  K+ A  +S+E   R+   EE    SE K + LE +V AL                 D++   +   ++++ S +Y    F WKI+E+S K   A  G  S ++S +FY+   GYK+C +LY  G     G  +S++F + +G YD  L WP  Q+++  L+DQ +       F  D         A  + F +PT+++N   G   FV
Sbjct:  270 EHFKCRSCQLVLRDAVQTPCGHRHCAGCVERSRSGGGRLCP----DCPE--IDEMDDRQIYPDMAVRREVHRLLVACRQTGCDWTGDLKTYQQNHER--SCLSRRVTTSEDDSSDAICKLQTEAAMLAQMDEIRQRVEDLQKEVGHKQQTGVEAMTRRVGEMEVGLQECRVQVQQLEEKVVEINRREPSTPGESSTVRVEQMRDNLKTLEHKLSTLEGMVAAIARDIE--KTSALLESQEATRRVQ--EERLKSSEDKVKALE-RVIAL----------------RDISMAEQDLRIQALESASYN-GVFLWKITEVSRKRHDAVSGRVSSLYSPAFYTSAHGYKMCCRLYLNGDGMGKGTHVSLFFVVMRGHYDALLRWPFKQKVTCILLDQNNLNHVIDAFRPD---------ASSSSFKRPTSEMNIASGFPSFV 668
[Close]
BLAST of TNF receptor-associated factor vs. TrEMBL
Match: A0A444UFS2 (TNF receptor-associated factor OS=Acipenser ruthenus OX=7906 GN=EOD39_5070 PE=3 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 2.683e-21
Identity = 123/487 (25.26%), Postives = 198/487 (40.66%), Query Frame = 2 
Query:  149 IVGLKYEGVWKLLRKYHFVIEIAKYEEFICIICRGLLKNAHQNKCGCRYCYECIIDFIHSDEKHCPGPTEECSQGLIDNNSDLQIDHTANRTVANILVPCPISP-CGVVIELKYMVHHVKLHKCHSKSSNFYDADFGDET---------------ASETCPSSHVNLLTDRIGF---LNEKVXXXXXXXXXXXXKVINIQSLVEEIKWKQDSAIENLKVINDKTTEKLRLARETLLSTEENDQKNQFIIRKLQQSIEEIKSKAARKSKEIEVRLTQLEEYYLQSETKQE---------KLEQKVFALXXXXXXXXXXXXHKTKHDLATCFELN-SVESIVSNNYRYAPFE---------WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQ---KSDT--VFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFV 1480
            +VG+ ++ V KL             E++IC  CR ++ N HQ  CG  +CY+CI  F+  +      PT      LI +N   Q D+   R V N+ + C  SP C   + L  +  H  L +C  +S     A   D                   E CP    +L+   +      +E  E   + + +     + I  L + +  K     E  KV+N    E   L ++          K   ++  +Q+S+E+ K + +     I+++L QL   + Q  ++ E          L+Q+V  + S        +   T+H       LN  VE +  N  R+   E         WKI +  +K  +A EG    IFS++FY+   GY++C + Y  G        LS+Y  + +GE+D  LPWP  Q ++L ++DQ   K+D   VF  D  S           F +P  ++N   G   FV
Sbjct:   30 LVGMNFQFVEKL------------EEQYICPSCRRVVLNPHQTGCGHIFCYQCITTFLECNS----APTCPIDNVLIKSNEVFQ-DNCCKREVLNLEIYCTNSPNCSSKVTLCRLKDH--LEQCQYESLQCTHAGCTDRMFRKDLKEHLKNICKYRVEPCPHCQTHLVLIHLKLKEHFHECPEVPVECMYKKYGCSLRIADLQKTLHLKHQEIQELTKVVNKLEKEIKPLMQQV--------TKTDNMLSNVQKSLEDQKDRVSI----IQIQLQQLTSAFNQDPSRSELGLLKRSVESLKQQVSVIESLKDRLTSLEGQYTRHSGL----LNIHVEQLSLNEERFRQLEAMSYNGKLIWKIEDYKKKKKEAVEGRVPSIFSQAFYTSRSGYRLCARAYLNGDGTGKRTHLSLYVVMMRGEFDSLLPWPFEQSVTLMILDQSGAKNDIVDVFRPDLNS---------NSFKRPVNEMNLAFGFPCFV 472
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Cavefish
Match: traf2b (TNF receptor associated factor 2 [Source:NCBI gene;Acc:103028261])

HSP 1 Score: 91.6633 bits (226), Expect = 2.918e-19
Identity = 67/244 (27.46%), Postives = 109/244 (44.67%), Query Frame = 2 
Query:  845 LQQSIEEIKSKAARKSKEIEVRLTQLEEYYLQSETKQEKLEQ---KVFALXXXXXXXXXXXXHKTKHDLATCFELNSVESIVSNNYRYAPFEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSD----TVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI 1555
            +QQ +  +++     ++E+E  + +LE    Q +  QEK+E    KV  L              T  DL       ++  +    Y    F WKIS+ S +   A  G    +FS +FYS   GYK+CL+LY  G     G  LS++F + +G+YD  L WP  Q+++L L+DQ +       F  D  S           F +P +++N   G   F  L  +      L +++I I+ +V +
Sbjct:  305 VQQKMTALENIVCVLNREVERTVLRLEALTRQQQLDQEKIENLSNKVRQLERTL----------TMRDLQLAESEQALRELQFCTYD-GVFVWKISDFSRRRQDALAGRAPAMFSPAFYSSKYGYKMCLRLYLNGDGTGRGSHLSLFFVVMRGKYDALLKWPFSQKVTLMLLDQNNREHIIDAFRPDISS---------TSFQRPISEMNIASGCPLFCPLAKLAGKSSYLRDDTIFIKAIVDL 528
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Cavefish
Match: traf2a (TNF receptor-associated factor 2-like [Source:NCBI gene;Acc:103036375])

HSP 1 Score: 86.6557 bits (213), Expect = 1.036e-17
Identity = 62/229 (27.07%), Postives = 103/229 (44.98%), Query Frame = 2 
Query:  890 SKEIEVRLTQLEEYYLQSETKQEKLE---QKVFALXXXXXXXXXXXXHKTKHDLATCFELNSVESIVSNNYRYAPFEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSD----TVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI 1555
            ++E+E   T +E Y  Q    Q+K+E    KV  L                 DL+       +  + +  Y    F WKIS+ S+K   A  G    +FS +FY+   GYK+CL++Y  G     G  LS++F + +G  D  L WP  Q+++L L+DQ +       F  D  S         + F +P +D+N   G   F  L+ + +    + +++I I+ +V +
Sbjct:  248 NREMERSATTMEAYNRQHRLDQDKIEILNNKVRQLERTVGL----------RDLSIVEMEGKMREMSAATYDGV-FVWKISDFSKKRQDAVAGRAPAMFSPAFYTSKYGYKMCLRIYLNGDGTGRGTHLSLFFVVMRGHSDALLKWPFNQKVTLMLLDQNNREHIIDAFRPDISS---------SSFQRPVSDMNIASGCPLFCPLSKLDSKNSYIRDDTIFIKAIVDL 456
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Cavefish
Match: traf1 (TNF receptor-associated factor 1-like [Source:NCBI gene;Acc:103045252])

HSP 1 Score: 85.8853 bits (211), Expect = 2.086e-17
Identity = 47/153 (30.72%), Postives = 80/153 (52.29%), Query Frame = 2 
Query: 1106 FEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSD----TVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILF-NNSIVIRCLV 1549
            F W+I ++S+K+ +A  G  S  +S +FY+   G+K+C++LY  G     G  +S++F I KG+YD  L WP  Q+++  LIDQ         F  D  S         A F +P +++N   G   F  L+ + + K     ++++ I+C+V
Sbjct:  394 FLWRICDVSQKLKEAATGQKSSQYSPAFYTSRYGFKVCMRLYMYGDGVGKGTHISLFFVIMKGDYDPLLSWPFKQKVTFFLIDQNQREHVVDAFRPDLRS---------ASFQRPVSEMNVASGCPLFFPLSKLRSPKHAYVKDDTLFIKCVV 537
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Cavefish
Match: traf1 (TNF receptor-associated factor 1-like [Source:NCBI gene;Acc:103045252])

HSP 1 Score: 85.8853 bits (211), Expect = 2.261e-17
Identity = 47/153 (30.72%), Postives = 80/153 (52.29%), Query Frame = 2 
Query: 1106 FEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSD----TVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILF-NNSIVIRCLV 1549
            F W+I ++S+K+ +A  G  S  +S +FY+   G+K+C++LY  G     G  +S++F I KG+YD  L WP  Q+++  LIDQ         F  D  S         A F +P +++N   G   F  L+ + + K     ++++ I+C+V
Sbjct:  371 FLWRICDVSQKLKEAATGQKSSQYSPAFYTSRYGFKVCMRLYMYGDGVGKGTHISLFFVIMKGDYDPLLSWPFKQKVTFFLIDQNQREHVVDAFRPDLRS---------ASFQRPVSEMNVASGCPLFFPLSKLRSPKHAYVKDDTLFIKCVV 514
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Cavefish
Match: traf2a (TNF receptor-associated factor 2-like [Source:NCBI gene;Acc:103036375])

HSP 1 Score: 85.1149 bits (209), Expect = 4.624e-17
Identity = 47/154 (30.52%), Postives = 78/154 (50.65%), Query Frame = 2 
Query: 1106 FEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSD----TVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI 1555
            F WKIS+ S+K   A  G    +FS +FY+   GYK+CL++Y  G     G  LS++F + +G  D  L WP  Q+++L L+DQ +       F  D  S         + F +P +D+N   G   F  L+ + +    + +++I I+ +V +
Sbjct:  425 FVWKISDFSKKRQDAVAGRAPAMFSPAFYTSKYGYKMCLRIYLNGDGTGRGTHLSLFFVVMRGHSDALLKWPFNQKVTLMLLDQNNREHIIDAFRPDISS---------SSFQRPVSDMNIASGCPLFCPLSKLDSKNSYIRDDTIFIKAIVDL 569
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000074.1 (pep scaffold:Pmarinus_7.0:GL482142:165593:173010:-1 gene:ENSPMAG00000000066.1 transcript:ENSPMAT00000000074.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 92.4337 bits (228), Expect = 2.470e-20
Identity = 46/146 (31.51%), Postives = 78/146 (53.42%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLV 1549
            WKI + + +   A  G    ++S+ FY+ P GY++C + Y  G     G  LS++F + +GEYD  LPWP  Q+++L L+DQ +     SD +      +   + F +PT+D+N   G   FV  +V   +   L +++I I+ +V
Sbjct:  329 WKIRDYARRKQDAVSGRTLSLYSQPFYTSPFGYRMCARAYLNGDGVGRGTHLSLFFVVMRGEYDALLPWPFRQRVTLTLLDQGAARRQLSDTFK----PDPASSSFRRPTSDMNIASGCPLFVS-HVALESASYLQDDTIFIKVVV 469
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002130.1 (pep scaffold:Pmarinus_7.0:GL484662:8457:12718:-1 gene:ENSPMAG00000001936.1 transcript:ENSPMAT00000002130.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 89.3521 bits (220), Expect = 1.884e-19
Identity = 43/146 (29.45%), Postives = 75/146 (51.37%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLV 1549
            WKI + + +  +A  G    ++S+ FY+ P GYK+C + Y  G     G  LS++F + +GEYD  L WP  ++++L L+DQ +     SD ++     +     F +P  D+N   G   FV  +V       L ++++ +R +V
Sbjct:  272 WKIRDYARRKQEAASGRTLSLYSQPFYTSPFGYKMCARAYLNGDGVGRGTHLSLFFVVMRGEYDALLEWPFRRRVTLSLLDQGAARRHLSDTFT----PDPASGSFRRPDGDMNVASGCPLFVS-HVALEGSSYLVDDTVFVRVVV 412
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Sea Lamprey
Match: traf6 (TNF receptor-associated factor 6 [Source:NCBI gene;Acc:554561])

HSP 1 Score: 59.3066 bits (142), Expect = 1.364e-9
Identity = 43/173 (24.86%), Postives = 83/173 (47.98%), Query Frame = 2 
Query: 1106 FEWKISELSEKVTK------AREGNN----SFIFSESFYSHPRGYKICLKL-------YPIGVDPAS----GR--FLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQ-KSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINS-GLGSSEFVKLNVIFNNKRILFNNSIVIRCLV 1549
            F W++   S  +        A +G +    + + S  FY+   GY++CL+L        P G    S    GR   L+++  + +G+YD  LPWP   +I + ++DQ + D+V  ++        + + + F +P+   N  G G   F+ +N + +++  L ++ +V+RC V
Sbjct:  395 FTWRVERFSRLLPGGLASGFASDGMHPDAPAVMHSSGFYTGVPGYRLCLRLQVLSGREVPAGAASPSVLLAGRQPHLALFVHLLQGDYDHRLPWPFKGRIRISVVDQCERDSVHVTEMMDA----DPQLSAFQRPSVHRNPKGFGYMNFMDINTL-HHRSYLRDDCLVVRCEV 562
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Nematostella
Match: EDO35237 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SM27])

HSP 1 Score: 98.2117 bits (243), Expect = 8.679e-22
Identity = 49/148 (33.11%), Postives = 82/148 (55.41%), Query Frame = 2 
Query: 1106 FEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLV 1549
            F WKI + +E+  +A+ GN + I+S  FY+H  GYK+C +L+P G   A   +LSI+  + +GEYD  LPWP  + I++ LIDQ  + +  S+ +    C+ +    F +PT +IN   G   F    V+   +  +  N + ++ +V
Sbjct:  382 FLWKIDKFTERYAEAKSGNATSIYSSPFYTHRFGYKLCARLFPNGDGSARDNYLSIFIALMRGEYDSILPWPFQETITITLIDQ-DNGMNISETFDPDPCSIS----FQRPTRNINVASGCPRFCSQTVL-RTRGYMNENCVFVKVVV 523
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Nematostella
Match: EDO45915 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRA4])

HSP 1 Score: 82.8037 bits (203), Expect = 4.415e-17
Identity = 42/126 (33.33%), Postives = 66/126 (52.38%), Query Frame = 2 
Query: 1106 FEWKISELSEKVTKAREGNNS-FIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFV 1480
            F WKI   S K+  A+ G  S  + SE+FY+ P GYK+C  ++  G+    GR++S+   ++ GE+D  L WP+  + +  L+DQ  DT    D  + +     E    S+P+ D   G G+  FV
Sbjct:  268 FIWKIQCFSRKLMDAKTGRASEPMISEAFYTSPHGYKMCAGVWLNGIGTGRGRYISVGLQVQSGEFDSILKWPLYPRYTFTLLDQSEDTKTKRDVVATF-----EPQSISRPSADRQLGKGARRFV 388
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Nematostella
Match: EDO49464 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG36])

HSP 1 Score: 81.6481 bits (200), Expect = 1.661e-16
Identity = 42/128 (32.81%), Postives = 64/128 (50.00%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQK-----SDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFV 1480
            WKI E + +  +   G    I+S  FY    GYK C ++YP G     G  LS++F + +GE+D  LPWP  Q+++ +L+DQ       DT F  D  S         + F +PT+++N   G   F+
Sbjct:  382 WKIDEYTRRYQEGVSGKTPSIYSPPFYVGRYGYKACARVYPNGDGMGKGSHLSLFFVLMRGEFDALLPWPFRQKVTFKLLDQDRVHDIGDT-FRPDPTS---------SSFKRPTSNMNIASGCPLFI 499
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Nematostella
Match: EDO49545 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGK7])

HSP 1 Score: 76.2554 bits (186), Expect = 5.734e-15
Identity = 52/146 (35.62%), Postives = 68/146 (46.58%), Query Frame = 2 
Query: 1061 NSVESIVSNNYRYAPFEWKISELSEKVTKAREGNNSF-IFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVI 1495
            N   S+ +N      F WKIS    +  +A        IFS  FY+   GYK+ LK Y  G D   G  LS+Y  I KG+YD  L WP  Q+I+  LIDQ       +   S        K VF++PT   N G+G+  FV   V+
Sbjct:  244 NESRSLCTNGI----FLWKISNYKHQYDQAVASPEELAIFSPPFYTSQYGYKLRLKAYLNGRDRGKGTHLSLYIIIMKGDYDALLEWPFKQKITFYLIDQSEKQNHRTHLLSPNRSLPNIKVVFNRPTVKENLGIGNPCFVPNEVL 385
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Nematostella
Match: EDO41243 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4Z7])

HSP 1 Score: 74.3294 bits (181), Expect = 3.542e-14
Identity = 40/125 (32.00%), Postives = 61/125 (48.80%), Query Frame = 2 
Query: 1106 FEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFV 1480
            F W+I + ++ + +A+ G    + SE F++   GYK+   LYP G        +S +  I +GEYD  L WP  + I + L+DQK + V            N     F +P T  N+G GS+ FV
Sbjct:  320 FVWRIPDFADSLRQAKLGLKVRLDSEPFFTEKNGYKLRATLYPNGTMSGKNTHISAFVSINRGEYDGILAWPFTRVIKITLLDQK-ENVLQRRNVEKTIIPNPSLENFHRPKTVSNNGRGSATFV 443

HSP 2 Score: 53.5286 bits (127), Expect = 1.140e-7
Identity = 35/103 (33.98%), Postives = 50/103 (48.54%), Query Frame = 2 
Query:  200 FVIEIAKYEEFICIICRGLLKNAHQNKCGCRYCYECIIDFIHSDEK-HCPGPTEECSQGLIDNNSDLQIDHTANRTVANILVPCPISPCGVVIELKYMVHHVK 505
            FVI +A  EE+ C IC   L+   Q KCG R+C EC+ +      +  CP   E      +D   D+ +D  A RT+ + LV CP   C    EL+ +  H +
Sbjct:   15 FVIAVA--EEYTCRICHLPLRVPVQTKCGHRFCKECLDEASRRKSRAECPMDREP-----LDLKKDVFLDKAAERTILSFLVKCPCKGCKWEAELRDLEAHAR 110
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Medaka
Match: traf2b (TNF receptor associated factor 2 [Source:NCBI gene;Acc:101159764])

HSP 1 Score: 86.2705 bits (212), Expect = 1.496e-17
Identity = 47/154 (30.52%), Postives = 76/154 (49.35%), Query Frame = 2 
Query: 1106 FEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSD----TVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI 1555
            F WKI++ S +  +A  G    +FS +FYS   GYK+CL+LY  G     G  LS++F + + +YD  L WP  Q+++L L+DQ +       F  D  S           F +P +++N   G   F  L  +      L +++I I+ +V +
Sbjct:  369 FVWKITDFSHRRQEAVSGRAPAMFSPAFYSSKYGYKMCLRLYLNGDGTGRGTHLSLFFVVMRSKYDALLKWPFSQKVTLMLLDQNNREHIIDAFRPDITS---------TSFQRPVSEMNIASGCPLFCPLAKLAGKSPYLRDDTIFIKAIVDL 513
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Medaka
Match: traf2b (TNF receptor associated factor 2 [Source:NCBI gene;Acc:101159764])

HSP 1 Score: 86.2705 bits (212), Expect = 1.531e-17
Identity = 47/154 (30.52%), Postives = 76/154 (49.35%), Query Frame = 2 
Query: 1106 FEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSD----TVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI 1555
            F WKI++ S +  +A  G    +FS +FYS   GYK+CL+LY  G     G  LS++F + + +YD  L WP  Q+++L L+DQ +       F  D  S           F +P +++N   G   F  L  +      L +++I I+ +V +
Sbjct:  376 FVWKITDFSHRRQEAVSGRAPAMFSPAFYSSKYGYKMCLRLYLNGDGTGRGTHLSLFFVVMRSKYDALLKWPFSQKVTLMLLDQNNREHIIDAFRPDITS---------TSFQRPVSEMNIASGCPLFCPLAKLAGKSPYLRDDTIFIKAIVDL 520
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Medaka
Match: ENSORLT00000020861.2 (TNF receptor-associated factor 2 [Source:NCBI gene;Acc:101167626])

HSP 1 Score: 83.5741 bits (205), Expect = 1.453e-16
Identity = 46/154 (29.87%), Postives = 77/154 (50.00%), Query Frame = 2 
Query: 1106 FEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSD----TVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI 1555
            F WKIS+ ++K   A  G    +FS +FY+   GYK+CL++Y  G     G  LS++F + +G  D  L WP  Q+++L L+DQ +       F  D  S         + F +P +D+N   G   F  L+ + +    + + +I I+ +V +
Sbjct:  422 FIWKISDFTKKRQDAMAGRAPAMFSPAFYTSKYGYKMCLRIYLNGDGTGRGTHLSLFFVVMRGHSDALLKWPFNQKVTLMLLDQNNREHIIDAFRPDISS---------SSFQRPVSDMNIASGCPLFCPLSKLDSKNSYIRDETIFIKAIVDL 566
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Medaka
Match: traf3 (TNF receptor associated factor 3 [Source:NCBI gene;Acc:101161601])

HSP 1 Score: 80.1073 bits (196), Expect = 1.180e-15
Identity = 39/128 (30.47%), Postives = 64/128 (50.00%), Query Frame = 2 
Query: 1112 WKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVI 1495
            WKI +   +   A  G    ++S+ FY+   GYK+C ++Y  G     G  LS++F + +GEYD  LPWP  Q+++L L+DQ        D +      +   + F +P  ++N   G   FV  +V+
Sbjct:  302 WKIRDYKRRKQDAVAGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVVMRGEYDALLPWPFKQKVTLMLMDQGPSRKHLGDAFK----PDPSSSSFRRPVAEMNIASGCPLFVSQSVL 425
[Close]
BLAST of TNF receptor-associated factor vs. Ensembl Medaka
Match: unm_sa808 (TNF receptor-associated factor 2 [Source:NCBI gene;Acc:101155367])

HSP 1 Score: 78.9518 bits (193), Expect = 3.750e-15
Identity = 43/150 (28.67%), Postives = 78/150 (52.00%), Query Frame = 2 
Query: 1106 FEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI 1555
            F W+IS+ ++K   A  G    +FS +FY+   GYK+CL++Y  G     G  LS++F + +G  D  L WP  Q+++L L+DQ S+     D +      +   + F +P +++N   G   F  L+ +      + +++I I+ +V +
Sbjct:  449 FIWRISDFAKKRQDAVAGRAPAMFSPAFYTSKYGYKMCLRIYLNGDGTGRGTHLSLFFVVMRGLSDALLKWPFNQKVTLMLLDQ-SNREHIIDAFR----PDVTSSSFQRPVSEMNIASGCPLFCPLSKLDAKNSYIRDDTIFIKAIVDL 593
[Close]
BLAST of TNF receptor-associated factor vs. Planmine SMEST
Match: SMESG000067158.1 (SMESG000067158.1)

HSP 1 Score: 910.598 bits (2352), Expect = 0.000e+0
Identity = 468/471 (99.36%), Postives = 469/471 (99.58%), Query Frame = 2 
Query:  143 MEIVGLKYEGVWKLLRKYHFVIEIAKYEEFICIICRGLLKNAHQNKCGCRYCYECIIDFIHSDEKHCPGPTEECSQGLIDNNSDLQIDHTANRTVANILVPCPISPCGVVIELKYMVHHVKLHKCHSKSSNFYDADFGDETASETCPSSHVNLLTDRIGFLNEKVXXXXXXXXXXXXKVINIQSLVEEIKWKQDSAIENLKVINDKTTEKLRLARETLLSTEENDQKNQFIIRKLQQSIEEIKSKAARKSKEIEVRLTQLEEYYLQSETKQEKLEQKVFALXXXXXXXXXXXXHKTKHDLATCFELNSVESIVSNNYRYAPFEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI 1555
            MEIVGLKYEGVWKLLRKYHFVIEIAKYEEFICIICRGLLKNAHQNKCGCRYCYECIIDFIHSDEKHCPGPTEEC QGLIDNNSDLQIDHTANRTVANILVPCPISPCGVVIELKYMVHHVKLHKCHSKSSNFYDADFGDETASETCPSSHVNLLTDRIGFLNEKVEQLQQELDESQEKVINIQSLVEEIKWKQDSAIENLKVINDKTTEKLRLARETLLSTEENDQKNQFIIRKL+QSIEEIKSK ARKSKEIEVRLTQLEEYYLQSETKQEKLEQKVFALSSQPPNPQPSKSHKTKHDLATCFELNSVESIVSNNYRYAPFEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI
Sbjct:    1 MEIVGLKYEGVWKLLRKYHFVIEIAKYEEFICIICRGLLKNAHQNKCGCRYCYECIIDFIHSDEKHCPGPTEECRQGLIDNNSDLQIDHTANRTVANILVPCPISPCGVVIELKYMVHHVKLHKCHSKSSNFYDADFGDETASETCPSSHVNLLTDRIGFLNEKVEQLQQELDESQEKVINIQSLVEEIKWKQDSAIENLKVINDKTTEKLRLARETLLSTEENDQKNQFIIRKLKQSIEEIKSKTARKSKEIEVRLTQLEEYYLQSETKQEKLEQKVFALSSQPPNPQPSKSHKTKHDLATCFELNSVESIVSNNYRYAPFEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI 471
[Close]
BLAST of TNF receptor-associated factor vs. Planmine SMEST
Match: SMESG000067158.1 (SMESG000067158.1)

HSP 1 Score: 908.286 bits (2346), Expect = 0.000e+0
Identity = 470/473 (99.37%), Postives = 471/473 (99.58%), Query Frame = 2 
Query:  137 FEMEIVGLKYEGVWKLLRKYHFVIEIAKYEEFICIICRGLLKNAHQNKCGCRYCYECIIDFIHSDEKHCPGPTEECSQGLIDNNSDLQIDHTANRTVANILVPCPISPCGVVIELKYMVHHVKLHKCHSKSSNFYDADFGDETASETCPSSHVNLLTDRIGFLNEKVXXXXXXXXXXXXKVINIQSLVEEIKWKQDSAIENLKVINDKTTEKLRLARETLLSTEENDQKNQFIIRKLQQSIEEIKSKAARKSKEIEVRLTQLEEYYLQSETKQEKLEQKVFALXXXXXXXXXXXXHKTKHDLATCFELNSVESIVSNNYRYAPFEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI 1555
            FEMEIVGLKYEGVWKLLRKYHFVIEIAKYEEFICIICRGLLKNAHQNKCGCRYCYECIIDFIHSDEKHCPGPTEEC QGLIDNNSDLQIDHTANRTVANILVPCPISPCGVVIELKYMVHHVKLHKCHSKSSNFYDADFGDETASETCPSSHVNLLTDRIGFLNEKVEQLQQELDESQEKVINIQSLVEEIKWKQDSAIENLKVINDKTTEKLRLARETLLSTEENDQKNQFIIRKL+QSIEEIKSK ARKSKEIEVRLTQLEEYYLQSETKQEKLEQKVFALSSQPPNPQPSKSHKTKHDLATCFELNSVESIVSNNYRYAPFEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI
Sbjct:  222 FEMEIVGLKYEGVWKLLRKYHFVIEIAKYEEFICIICRGLLKNAHQNKCGCRYCYECIIDFIHSDEKHCPGPTEECRQGLIDNNSDLQIDHTANRTVANILVPCPISPCGVVIELKYMVHHVKLHKCHSKSSNFYDADFGDETASETCPSSHVNLLTDRIGFLNEKVEQLQQELDESQEKVINIQSLVEEIKWKQDSAIENLKVINDKTTEKLRLARETLLSTEENDQKNQFIIRKLKQSIEEIKSKTARKSKEIEVRLTQLEEYYLQSETKQEKLEQKVFALSSQPPNPQPSKSHKTKHDLATCFELNSVESIVSNNYRYAPFEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVGI 694

HSP 2 Score: 357.836 bits (917), Expect = 1.484e-115
Identity = 181/186 (97.31%), Postives = 182/186 (97.85%), Query Frame = 2 
Query:  143 MEIVGLKYEGVWKLLRKYHFVIEIAKYEEFICIICRGLLKNAHQNKCGCRYCYECIIDFIHSDEKHCPGPTEECSQGLIDNNSDLQIDHTANRTVANILVPCPISPCGVVIELKYMVHHVKLHKCHSKSSNFYDADFGDETASETCPSSHVNLLTDRIGFLNEKVXXXXXXXXXXXXKVINIQSLV 700
            MEIVGLKYEGVWKLLRKYHFVIEIAKYEEFICIICRGLLKNAHQNKCGCRYCYECIIDFIHSDEKHCPGPTEEC QGLIDNNSDLQIDHTANRTVANILVPCPISPCGVVIELKYMVHHVKLHKCHSKSSNFYDADFGDETASETCPSSHVNLLTDRIGFLNEKVEQLQQELDESQ K +NI SLV
Sbjct:    1 MEIVGLKYEGVWKLLRKYHFVIEIAKYEEFICIICRGLLKNAHQNKCGCRYCYECIIDFIHSDEKHCPGPTEECRQGLIDNNSDLQIDHTANRTVANILVPCPISPCGVVIELKYMVHHVKLHKCHSKSSNFYDADFGDETASETCPSSHVNLLTDRIGFLNEKVEQLQQELDESQVKKLNI-SLV 185
[Close]
BLAST of TNF receptor-associated factor vs. Planmine SMEST
Match: SMESG000070126.1 (SMESG000070126.1)

HSP 1 Score: 229.18 bits (583), Expect = 3.171e-69
Identity = 146/475 (30.74%), Postives = 243/475 (51.16%), Query Frame = 2 
Query:  149 IVGLKYEGVWKLLRKYHFVIEIAKYEEFICIICRGLLKNAHQNKCGCRYCYECIIDFIHSDEKHCPGPTEECSQGLIDNNSDLQIDHTANRTVANILVPCPISPCGVVIELKYMVHHVKLHKCHSKSSNFYDADFGDETASETC--PSSHVNLLTDRIGFLNEKVXXXXXXXXXXXXKVINIQSLVEEIKWKQDSAIENLKVINDKTTEKLRLARETLLSTEENDQKNQFIIRKLQQSIEEIKSKAARKSKEIEVRLTQLEEYYLQ--SETKQEKLEQKVFALXXXXXXXXXXX---XHKTKHDLATCFELNSVESIVSNNYRYAPFEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVG 1552
            +   KYE +WKLL +Y F  +    +EF+C+IC  +LKN +Q+ CGCR+C ECI+D+++  E HCPG +E+C   LI+ N DL  D+  +R ++ + V CP + C    EL+ + +H+  HK     +N              C  PS+H+ L+ + +                  +++     LV E       +  NLK  N+  +  L L      S +E + K+  +I  L+  + E++     K +E+   L + +E  +Q  SE +  K   +VF+ + +P   +  +     K K       +L  ++ I +++  YAPFEWKI +   K  KA     +++ S  FYSH  GYK+C+ ++P G     G ++S++  + KGE+D  L WPM   + + L+DQ SD+ F   ++ Y S     K  +S+P    N G+G S+F++LN +FNN RI   +SI+++C VG
Sbjct:    1 MANAKYERIWKLLDEYEFAYDSLLSDEFLCVICNSILKNPYQSPCGCRFCMECIVDYLNDKETHCPGSSEDCKDQLINFNGDLHADYVISRKISQVKVKCPKALCDYRSELQNIGNHISSHKESCPYTNI------------GCQYPSAHMGLIVEHL-----------------HDQIYEHGKLVTE-------SFANLK--NEVASLTLALKH----SKQELENKDNLLI-DLENRLNELQGTREIKIQELTETLDKCKEENVQLSSELRTLKSVSEVFSSNQKPKKGEKVQDEDLEKYKVISKNITQLIPMKPISTDHISYAPFEWKIRDF--KALKASTVIPTYVISRPFYSHVNGYKLCMTIHPSGTGIGLGSYISVFIHVMKGEFDSNLAWPMKLDLRICLVDQISDSYFVDKKFDYSSAILRNKHKYSRPFCVKNIGIGESQFIQLNALFNNSRIYLEDSIILKCFVG 430
[Close]
BLAST of TNF receptor-associated factor vs. Planmine SMEST
Match: SMESG000070126.1 (SMESG000070126.1)

HSP 1 Score: 228.794 bits (582), Expect = 9.775e-69
Identity = 146/475 (30.74%), Postives = 243/475 (51.16%), Query Frame = 2 
Query:  149 IVGLKYEGVWKLLRKYHFVIEIAKYEEFICIICRGLLKNAHQNKCGCRYCYECIIDFIHSDEKHCPGPTEECSQGLIDNNSDLQIDHTANRTVANILVPCPISPCGVVIELKYMVHHVKLHKCHSKSSNFYDADFGDETASETC--PSSHVNLLTDRIGFLNEKVXXXXXXXXXXXXKVINIQSLVEEIKWKQDSAIENLKVINDKTTEKLRLARETLLSTEENDQKNQFIIRKLQQSIEEIKSKAARKSKEIEVRLTQLEEYYLQ--SETKQEKLEQKVFALXXXXXXXXXXX---XHKTKHDLATCFELNSVESIVSNNYRYAPFEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLVG 1552
            +   KYE +WKLL +Y F  +    +EF+C+IC  +LKN +Q+ CGCR+C ECI+D+++  E HCPG +E+C   LI+ N DL  D+  +R ++ + V CP + C    EL+ + +H+  HK     +N              C  PS+H+ L+ + +                  +++     LV E       +  NLK  N+  +  L L      S +E + K+  +I  L+  + E++     K +E+   L + +E  +Q  SE +  K   +VF+ + +P   +  +     K K       +L  ++ I +++  YAPFEWKI +   K  KA     +++ S  FYSH  GYK+C+ ++P G     G ++S++  + KGE+D  L WPM   + + L+DQ SD+ F   ++ Y S     K  +S+P    N G+G S+F++LN +FNN RI   +SI+++C VG
Sbjct:   27 MANAKYERIWKLLDEYEFAYDSLLSDEFLCVICNSILKNPYQSPCGCRFCMECIVDYLNDKETHCPGSSEDCKDQLINFNGDLHADYVISRKISQVKVKCPKALCDYRSELQNIGNHISSHKESCPYTNI------------GCQYPSAHMGLIVEHL-----------------HDQIYEHGKLVTE-------SFANLK--NEVASLTLALKH----SKQELENKDNLLI-DLENRLNELQGTREIKIQELTETLDKCKEENVQLSSELRTLKSVSEVFSSNQKPKKGEKVQDEDLEKYKVISKNITQLIPMKPISTDHISYAPFEWKIRDF--KALKASTVIPTYVISRPFYSHVNGYKLCMTIHPSGTGIGLGSYISVFIHVMKGEFDSNLAWPMKLDLRICLVDQISDSYFVDKKFDYSSAILRNKHKYSRPFCVKNIGIGESQFIQLNALFNNSRIYLEDSIILKCFVG 456
[Close]
BLAST of TNF receptor-associated factor vs. Planmine SMEST
Match: SMESG000070126.1 (SMESG000070126.1)

HSP 1 Score: 226.098 bits (575), Expect = 1.618e-65
Identity = 145/474 (30.59%), Postives = 242/474 (51.05%), Query Frame = 2 
Query:  149 IVGLKYEGVWKLLRKYHFVIEIAKYEEFICIICRGLLKNAHQNKCGCRYCYECIIDFIHSDEKHCPGPTEECSQGLIDNNSDLQIDHTANRTVANILVPCPISPCGVVIELKYMVHHVKLHKCHSKSSNFYDADFGDETASETC--PSSHVNLLTDRIGFLNEKVXXXXXXXXXXXXKVINIQSLVEEIKWKQDSAIENLKVINDKTTEKLRLARETLLSTEENDQKNQFIIRKLQQSIEEIKSKAARKSKEIEVRLTQLEEYYLQ--SETKQEKLEQKVFALXXXXXXXXXXX---XHKTKHDLATCFELNSVESIVSNNYRYAPFEWKISELSEKVTKAREGNNSFIFSESFYSHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISLQLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNVIFNNKRILFNNSIVIRCLV 1549
            +   KYE +WKLL +Y F  +    +EF+C+IC  +LKN +Q+ CGCR+C ECI+D+++  E HCPG +E+C   LI+ N DL  D+  +R ++ + V CP + C    EL+ + +H+  HK     +N              C  PS+H+ L+ + +                  +++     LV E       +  NLK  N+  +  L L      S +E + K+  +I  L+  + E++     K +E+   L + +E  +Q  SE +  K   +VF+ + +P   +  +     K K       +L  ++ I +++  YAPFEWKI +   K  KA     +++ S  FYSH  GYK+C+ ++P G     G ++S++  + KGE+D  L WPM   + + L+DQ SD+ F   ++ Y S     K  +S+P    N G+G S+F++LN +FNN RI   +SI+++C V
Sbjct:    1 MANAKYERIWKLLDEYEFAYDSLLSDEFLCVICNSILKNPYQSPCGCRFCMECIVDYLNDKETHCPGSSEDCKDQLINFNGDLHADYVISRKISQVKVKCPKALCDYRSELQNIGNHISSHKESCPYTNI------------GCQYPSAHMGLIVEHL-----------------HDQIYEHGKLVTE-------SFANLK--NEVASLTLALKH----SKQELENKDNLLI-DLENRLNELQGTREIKIQELTETLDKCKEENVQLSSELRTLKSVSEVFSSNQKPKKGEKVQDEDLEKYKVISKNITQLIPMKPISTDHISYAPFEWKIRDF--KALKASTVIPTYVISRPFYSHVNGYKLCMTIHPSGTGIGLGSYISVFIHVMKGEFDSNLAWPMKLDLRICLVDQISDSYFVDKKFDYSSAILRNKHKYSRPFCVKNIGIGESQFIQLNALFNNSRIYLEDSIILKCFV 429

HSP 2 Score: 73.559 bits (179), Expect = 1.874e-13
Identity = 38/121 (31.40%), Postives = 67/121 (55.37%), Query Frame = 2 
Query:  149 IVGLKYEGVWKLLR--KYHFVIEIAKYEEFICIICRGLLKNAHQNKCGCRYCYECIIDFIHSDEKHCPGPTEECSQGLIDNNSDLQIDHTANRTVANILVPCPISPCGVVIELKYMVHHVK 505
            +V    E ++K+++  K+  V EI++  E  C IC  L+  A    CGC +C +CI  ++   +K CPG +  C   +I+  +D+ I++ AN  ++ I+V CP   C    EL+ + +HV+
Sbjct:  429 VVDKMSESIYKIMKEKKFEIVSEISR--ELKCNICNELMIKACSAPCGCCFCSDCIEQYLDGADKFCPGTSRRCQMKMINYANDIVIENRANVKISEIIVKCPRQNCEFEDELEMIANHVR 547
The following BLAST results are available for this feature:
BLAST of TNF receptor-associated factor vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TRAF36.963e-1832.19TNF receptor associated factor 3 [Source:HGNC Symb... [more]
TRAF39.825e-1832.19TNF receptor associated factor 3 [Source:HGNC Symb... [more]
TRAF39.825e-1832.19TNF receptor associated factor 3 [Source:HGNC Symb... [more]
TRAF31.159e-1732.19TNF receptor associated factor 3 [Source:HGNC Symb... [more]
TRAF31.159e-1732.19TNF receptor associated factor 3 [Source:HGNC Symb... [more]
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BLAST of TNF receptor-associated factor vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
trf-11.699e-1229.71TNF Receptor Associated Factor (TRAF) homolog; TRF... [more]
trf-27.471e-832.10TNF Receptor Associated Factor (TRAF) homolog [So... [more]
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BLAST of TNF receptor-associated factor vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Traf43.269e-925.31gene:FBgn0026319 transcript:FBtr0334401[more]
Traf45.972e-925.31gene:FBgn0026319 transcript:FBtr0077429[more]
Traf62.062e-824.67gene:FBgn0265464 transcript:FBtr0071188[more]
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BLAST of TNF receptor-associated factor vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
traf2b7.787e-1932.47Tnf receptor-associated factor 2b [Source:ZFIN;Acc... [more]
traf2b7.787e-1932.47Tnf receptor-associated factor 2b [Source:ZFIN;Acc... [more]
traf2b1.090e-1832.47Tnf receptor-associated factor 2b [Source:ZFIN;Acc... [more]
BX901915.15.360e-1830.52pep chromosome:GRCz11:CHR_ALT_CTG5_1_18:28764891:2... [more]
AL844899.15.360e-1830.52pep chromosome:GRCz11:CHR_ALT_CTG5_2_4:28756846:28... [more]
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BLAST of TNF receptor-associated factor vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hspb62.366e-1932.00heat shock protein family B (small) member 6 [Sour... [more]
ssh25.498e-1829.28slingshot protein phosphatase 2 [Source:Xenbase;Ac... [more]
ssh26.110e-1829.28slingshot protein phosphatase 2 [Source:Xenbase;Ac... [more]
slc12a14.392e-1730.00solute carrier family 12 member 1 [Source:Xenbase;... [more]
slc12a16.300e-1733.33solute carrier family 12 member 1 [Source:Xenbase;... [more]
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BLAST of TNF receptor-associated factor vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Traf36.786e-1832.19TNF receptor-associated factor 3 [Source:MGI Symbo... [more]
Traf36.786e-1832.19TNF receptor-associated factor 3 [Source:MGI Symbo... [more]
Traf37.799e-1832.19TNF receptor-associated factor 3 [Source:MGI Symbo... [more]
Traf55.524e-1730.66TNF receptor-associated factor 5 [Source:MGI Symbo... [more]
Traf55.524e-1730.66TNF receptor-associated factor 5 [Source:MGI Symbo... [more]
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BLAST of TNF receptor-associated factor vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q60803|TRAF3_MOUSE5.455e-1732.19TNF receptor-associated factor 3 OS=Mus musculus O... [more]
sp|Q13114|TRAF3_HUMAN5.567e-1732.19TNF receptor-associated factor 3 OS=Homo sapiens O... [more]
sp|O00463|TRAF5_HUMAN5.229e-1632.35TNF receptor-associated factor 5 OS=Homo sapiens O... [more]
sp|Q12933|TRAF2_HUMAN1.903e-1528.57TNF receptor-associated factor 2 OS=Homo sapiens O... [more]
sp|P39429|TRAF2_MOUSE2.080e-1529.87TNF receptor-associated factor 2 OS=Mus musculus O... [more]
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BLAST of TNF receptor-associated factor vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
T1E1011.022e-2625.56TRAF-6 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1[more]
D5JG596.007e-2332.77TNF receptor-associated factor-2-like protein A OS... [more]
A0A0F7GY081.238e-2239.01TNF receptor associated factor 5 (Fragment) OS=Sch... [more]
A0A1S3K6C11.345e-2125.80TNF receptor-associated factor 2 OS=Lingula unguis... [more]
A0A444UFS22.683e-2125.26TNF receptor-associated factor OS=Acipenser ruthen... [more]
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BLAST of TNF receptor-associated factor vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
traf2b2.918e-1927.46TNF receptor associated factor 2 [Source:NCBI gene... [more]
traf2a1.036e-1727.07TNF receptor-associated factor 2-like [Source:NCBI... [more]
traf12.086e-1730.72TNF receptor-associated factor 1-like [Source:NCBI... [more]
traf12.261e-1730.72TNF receptor-associated factor 1-like [Source:NCBI... [more]
traf2a4.624e-1730.52TNF receptor-associated factor 2-like [Source:NCBI... [more]
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BLAST of TNF receptor-associated factor vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSPMAT00000000074.12.470e-2031.51pep scaffold:Pmarinus_7.0:GL482142:165593:173010:-... [more]
ENSPMAT00000002130.11.884e-1929.45pep scaffold:Pmarinus_7.0:GL484662:8457:12718:-1 g... [more]
traf61.364e-924.86TNF receptor-associated factor 6 [Source:NCBI gene... [more]
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BLAST of TNF receptor-associated factor vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of TNF receptor-associated factor vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO352378.679e-2233.11Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO459154.415e-1733.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO494641.661e-1632.81Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO495455.734e-1535.62Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO412433.542e-1432.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of TNF receptor-associated factor vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
traf2b1.496e-1730.52TNF receptor associated factor 2 [Source:NCBI gene... [more]
traf2b1.531e-1730.52TNF receptor associated factor 2 [Source:NCBI gene... [more]
ENSORLT00000020861.21.453e-1629.87TNF receptor-associated factor 2 [Source:NCBI gene... [more]
traf31.180e-1530.47TNF receptor associated factor 3 [Source:NCBI gene... [more]
unm_sa8083.750e-1528.67TNF receptor-associated factor 2 [Source:NCBI gene... [more]
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BLAST of TNF receptor-associated factor vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000067158.10.000e+099.36SMESG000067158.1[more]
SMESG000067158.11.484e-11599.37SMESG000067158.1[more]
SMESG000070126.13.171e-6930.74SMESG000070126.1[more]
SMESG000070126.19.775e-6930.74SMESG000070126.1[more]
SMESG000070126.11.618e-6530.59SMESG000070126.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027755 ID=SMED30027755|Name=TNF receptor-associated factor|organism=Schmidtea mediterranea sexual|type=transcript|length=1607bp
AAACATTTTTATTTAAATATTATATTTTTCTATATTGACTAATTTTTATT
CATTTATACCTGACTAAATATATATTTTACTTTACTGAATTTTGTTACAA
TCAAAGTTTTATTGGAATTCTTATAGAGTTTGAAAATTTGAAATGGAAAT
TGTCGGCCTTAAATATGAAGGAGTTTGGAAACTTTTACGAAAATATCATT
TCGTAATTGAAATCGCTAAATACGAGGAATTTATTTGTATAATCTGTCGT
GGCCTTCTCAAAAATGCTCATCAGAATAAATGTGGATGCCGCTATTGTTA
CGAGTGTATTATTGATTTCATTCACAGTGATGAGAAACACTGTCCCGGAC
CAACTGAAGAATGCAGTCAAGGATTGATTGATAACAATTCCGATTTACAA
ATCGACCACACTGCAAACAGAACTGTAGCAAATATCCTCGTCCCATGCCC
GATATCTCCGTGTGGGGTTGTGATTGAATTGAAATACATGGTCCATCACG
TTAAGTTACACAAATGTCACTCAAAATCATCTAATTTTTACGATGCTGAT
TTCGGTGATGAAACTGCGTCTGAAACCTGTCCAAGTTCTCACGTAAATTT
ACTGACTGATCGTATTGGGTTCCTTAACGAAAAAGTGGAACAATTACAGC
AAGAACTAGACGAATCTCAAGAAAAGGTCATCAACATCCAATCGCTAGTA
GAGGAGATCAAATGGAAGCAAGATAGTGCAATTGAAAATTTGAAAGTAAT
CAATGATAAAACGACGGAAAAACTGAGGTTGGCCCGGGAAACATTGCTGA
GCACTGAAGAAAACGATCAAAAGAATCAATTTATAATAAGAAAACTCCAA
CAGTCTATCGAAGAAATAAAATCGAAAGCTGCCCGTAAAAGCAAGGAAAT
CGAAGTAAGATTAACGCAATTGGAAGAATATTATCTGCAATCCGAAACCA
AACAGGAAAAACTCGAACAAAAAGTGTTTGCTCTGTCGTCCCAACCCCCA
AACCCACAGCCATCGAAATCTCATAAAACCAAACATGACCTGGCCACTTG
TTTTGAACTCAACTCTGTTGAATCTATCGTTTCTAACAATTATAGATACG
CGCCTTTTGAGTGGAAGATATCTGAGCTGTCGGAAAAAGTAACTAAAGCC
AGAGAAGGAAATAACTCTTTCATTTTTAGCGAGTCGTTTTATTCTCATCC
AAGAGGCTATAAAATCTGTCTTAAACTTTATCCAATAGGAGTCGACCCCG
CATCGGGTAGGTTTTTATCGATTTATTTCTGTATTAAAAAAGGCGAATAC
GATGACTTCTTACCTTGGCCGATGATGCAACAAATTTCTCTGCAGTTGAT
TGATCAGAAATCTGATACCGTTTTCTCTTCCGATGAATACAGTTACTATT
CTTGCAATAATGCGGAAAAAGCGGTATTTTCGCAACCGACCACTGACATA
AATAGCGGACTGGGTTCGTCTGAGTTCGTCAAGTTGAATGTTATATTTAA
TAACAAAAGAATTCTTTTCAATAATTCCATTGTTATTAGGTGCTTGGTAG
GAATTTGATTGGTTTGATATTTGTATATTTAATAAATTTTGCTTTTTGGA
AAAAAAA
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protein sequence of SMED30027755-orf-1

>SMED30027755-orf-1 ID=SMED30027755-orf-1|Name=SMED30027755-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=472bp
MEIVGLKYEGVWKLLRKYHFVIEIAKYEEFICIICRGLLKNAHQNKCGCR
YCYECIIDFIHSDEKHCPGPTEECSQGLIDNNSDLQIDHTANRTVANILV
PCPISPCGVVIELKYMVHHVKLHKCHSKSSNFYDADFGDETASETCPSSH
VNLLTDRIGFLNEKVEQLQQELDESQEKVINIQSLVEEIKWKQDSAIENL
KVINDKTTEKLRLARETLLSTEENDQKNQFIIRKLQQSIEEIKSKAARKS
KEIEVRLTQLEEYYLQSETKQEKLEQKVFALSSQPPNPQPSKSHKTKHDL
ATCFELNSVESIVSNNYRYAPFEWKISELSEKVTKAREGNNSFIFSESFY
SHPRGYKICLKLYPIGVDPASGRFLSIYFCIKKGEYDDFLPWPMMQQISL
QLIDQKSDTVFSSDEYSYYSCNNAEKAVFSQPTTDINSGLGSSEFVKLNV
IFNNKRILFNNSIVIRCLVGI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0007165signal transduction
GO:0042981regulation of apoptotic process
GO:0016567protein ubiquitination
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR043211TNF_rcpt-assoc_TRAF
IPR002083MATH/TRAF_dom
IPR013083Znf_RING/FYVE/PHD
IPR008974TRAF-like
IPR001841Znf_RING
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0008270zinc ion binding
GO:0004842ubiquitin-protein transferase activity
GO:0046872metal ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 235..255
NoneNo IPR availableCOILSCoilCoilcoord: 154..188
NoneNo IPR availableCOILSCoilCoilcoord: 260..280
NoneNo IPR availableCOILSCoilCoilcoord: 200..227
NoneNo IPR availablePANTHERPTHR10131:SF129TNF RECEPTOR-ASSOCIATED FACTOR 6coord: 28..472
NoneNo IPR availableSUPERFAMILYSSF57850RING/U-boxcoord: 30..105
IPR002083MATH/TRAF domainSMARTSM00061math_3coord: 331..454
e-value: 5.1E-6
score: 36.0
IPR002083MATH/TRAF domainPROSITEPS50144MATHcoord: 322..472
score: 17.118
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 22..137
e-value: 1.7E-10
score: 42.9
IPR008974TRAF-likeGENE3DG3DSA:2.60.210.10coord: 290..474
e-value: 1.1E-39
score: 137.8
IPR008974TRAF-likeSUPERFAMILYSSF49599TRAF domain-likecoord: 325..472
IPR043211TNF receptor-associated factor TRAFPANTHERPTHR10131TNF RECEPTOR ASSOCIATED FACTORcoord: 28..472
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 35..71
score: 8.624