SMED30027753

Overview
NameSMED30027753
Smed IDSMED30027753
Length (bp)2267
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30027753 (SMED30027753) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30027753 (SMED30027753) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30027753 (SMED30027753) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30027753 (SMED30027753) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30027753

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30027753h1SMcG0015591 dd_Smed_v4_11917_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30027753h1SMcG0015591 dd_Smed_v4_11917_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30027753h1SMcG0015591 dd_Smed_v4_11917_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30027753h1SMcG0015591 dd_Smed_v4_11917_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30027753h1SMcG0015591 dd_Smed_v4_11917_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30027753h1SMcG0015591 dd_Smed_v4_11917_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30027753h1SMcG0015591 dd_Smed_v4_11917_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30027753h1SMcG0015591 dd_Smed_v6_11917_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30027753 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000911:39257..41439 +10846
Homology
[Close]
BLAST of SMED30027753 vs. Ensembl Human
Match: SLC18A1 (solute carrier family 18 member A1 [Source:HGNC Symbol;Acc:HGNC:10934])

HSP 1 Score: 82.0333 bits (201), Expect = 1.263e-15
Identity = 49/203 (24.14%), Postives = 98/203 (48.28%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P KV P  +    +  +LK P++L+    +  AN  +  L  TLP WM   +C+ + Q GL +    + Y+ G  L  +      +++    +I  ++ G  L+ +P +H+   +  P  G+   +G+V+  + P+  HL D    S YG++ ++   +  +    G  + G I +  GF  L +  G++N+++  L + L++
Sbjct:  280 PSKVSPESAKGTPLFMLLKDPYILVAAGSICFANMGVAILEPTLPIWMMQTMCSPKWQLGLAFLPASVSYLIGTNLFGVLANKMGRWL--CSLIGMLVVGTSLLCVPLAHNIFGLIGPNAGLGLAIGMVDSSMMPIMGHLVDLRHTSVYGSVYAIADVAFCMGFAIGPSTGGAIVKAIGFPWLMVITGVINIVYAPLCYYLRS 480
[Close]
BLAST of SMED30027753 vs. Ensembl Human
Match: SLC18A1 (solute carrier family 18 member A1 [Source:HGNC Symbol;Acc:HGNC:10934])

HSP 1 Score: 82.0333 bits (201), Expect = 1.263e-15
Identity = 49/203 (24.14%), Postives = 98/203 (48.28%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P KV P  +    +  +LK P++L+    +  AN  +  L  TLP WM   +C+ + Q GL +    + Y+ G  L  +      +++    +I  ++ G  L+ +P +H+   +  P  G+   +G+V+  + P+  HL D    S YG++ ++   +  +    G  + G I +  GF  L +  G++N+++  L + L++
Sbjct:  280 PSKVSPESAKGTPLFMLLKDPYILVAAGSICFANMGVAILEPTLPIWMMQTMCSPKWQLGLAFLPASVSYLIGTNLFGVLANKMGRWL--CSLIGMLVVGTSLLCVPLAHNIFGLIGPNAGLGLAIGMVDSSMMPIMGHLVDLRHTSVYGSVYAIADVAFCMGFAIGPSTGGAIVKAIGFPWLMVITGVINIVYAPLCYYLRS 480
[Close]
BLAST of SMED30027753 vs. Ensembl Human
Match: SLC18A2 (solute carrier family 18 member A2 [Source:HGNC Symbol;Acc:HGNC:10935])

HSP 1 Score: 80.1073 bits (196), Expect = 4.951e-15
Identity = 49/203 (24.14%), Postives = 100/203 (49.26%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P +V+P       +  +LK P++LI    +  AN  +  L   LP WM   +C+ + Q G+ +    I Y+ G  +  +      +++    ++  +I G+ ++ +PF+ +   +  P  G+ F +G+V+  + P+  +L D    S YG++ ++   +  +    G  + G IA+  GF  L   +GI++++F  L F L++
Sbjct:  272 PSRVQPESQKGTPLTTLLKDPYILIAAGSICFANMGIAMLEPALPIWMMETMCSRKWQLGVAFLPASISYLIGTNIFGILAHKMGRWL--CALLGMIIVGVSILCIPFAKNIYGLIAPNFGVGFAIGMVDSSMMPIMGYLVDLRHVSVYGSVYAIADVAFCMGYAIGPSAGGAIAKAIGFPWLMTIIGIIDILFAPLCFFLRS 472
[Close]
BLAST of SMED30027753 vs. Ensembl Human
Match: SLC18A3 (solute carrier family 18 member A3 [Source:HGNC Symbol;Acc:HGNC:10936])

HSP 1 Score: 68.1662 bits (165), Expect = 3.447e-11
Identity = 43/179 (24.02%), Postives = 83/179 (46.37%), Query Frame = -3 
Query:  835 IMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGL-VTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILN 1368
            I R++  P++ +    L   N  +  L  T+ +WM   + A+E + G+ W   F+ +V GV L V L  +Y P   +    +   + G    ++P    +  + + + G+ F + +V+  + P    L D    S YG++ ++   S ++    G + +G I    GF  LS+ +G+ N
Sbjct:  282 IHRLMLDPYIAVVAGALTTCNIPLAFLEPTIATWMKHTMAASEWEMGMAWLPAFVPHVLGVYLTVRLAARY-PHLQWLYGALGLAVIGASSCIVPACRSFAPLVVSLCGLCFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYSVAYALGPIVAGHIVHSLGFEQLSLGMGLAN 459
[Close]
BLAST of SMED30027753 vs. Ensembl Human
Match: SLC18A1 (solute carrier family 18 member A1 [Source:HGNC Symbol;Acc:HGNC:10934])

HSP 1 Score: 67.0106 bits (162), Expect = 5.803e-11
Identity = 42/172 (24.42%), Postives = 80/172 (46.51%), Query Frame = -3 
Query:  892 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSG 1407
            P KV P  +    +  +LK P++L+    +  AN  +  L  TLP WM   +C+ + Q GL +    + Y+ G  L  +      +++    +I  ++ G  L+ +P +H+   +  P  G+   +G+V+  + P+  HL D    S YG++ ++   +  +    G   SG
Sbjct:  280 PSKVSPESAKGTPLFMLLKDPYILVAAGSICFANMGVAILEPTLPIWMMQTMCSPKWQLGLAFLPASVSYLIGTNLFGVLANKMGRWL--CSLIGMLVVGTSLLCVPLAHNIFGLIGPNAGLGLAIGMVDSSMMPIMGHLVDLRHTSVYGSVYAIADVAFCMGFAIGYSESG 449
[Close]
BLAST of SMED30027753 vs. Ensembl Celegans
Match: unc-17 (Vesicular acetylcholine transporter unc-17 [Source:UniProtKB/Swiss-Prot;Acc:P34711])

HSP 1 Score: 70.8626 bits (172), Expect = 1.779e-12
Identity = 46/191 (24.08%), Postives = 90/191 (47.12%), Query Frame = -3 
Query:  799 IMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATE-IQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1368
            + R+   PF+  C   L +AN S+  L  T+ +WM   +  T     G++W   F  +V GV +     +  P   +   ++   + GI    +P++     + IP+  + F + +++  + P+  HL D    S YG++ ++   S ++   FG + +G I   +GF  L+I +   N+ +  + FLL+ 
Sbjct:  260 MWRLFMDPFIACCSGALIMANVSLAFLEPTITTWMSEMMPDTPGWLVGVIWLPPFFPHVLGVYVTVKMLRAFPHHTWAIAMVGLAMEGIACFAIPYTTSVMQLVIPLSFVCFGIALIDTSLLPMLGHLVDTRHVSVYGSVYAIADISYSLAYAFGPIIAGWIVTNWGFTALNIIIFATNVTYAPVLFLLRK 450
[Close]
BLAST of SMED30027753 vs. Ensembl Celegans
Match: cat-1 (pep chromosome:WBcel235:X:5705719:5710625:-1 gene:WBGene00000295.1 transcript:W01C8.6a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:cat-1)

HSP 1 Score: 63.5438 bits (153), Expect = 3.663e-10
Identity = 43/191 (22.51%), Postives = 89/191 (46.60%), Query Frame = -3 
Query:  799 SIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1371
            SI ++ K P++++    + I N  +  L  +LP WM     A  +++G  +    I Y+ G  +         +++  +  I  ++ G  L+ +P +     + IP   + F +G+++  +FPL  +L D      YG++ ++   +       G   SGP+ +  GF  +   + +++ ++  L FLLKN
Sbjct:  312 SIKQLAKDPYIIVAAGAITIGNLGIAMLEPSLPLWMMESWGANSLERGAAFLPASISYLIGTNIFGPLAHRIGRWL--SSFIGLVVIGFSLLSIPSATSVAGLIIPHALLGFSIGMIDASMFPLMGYLVDIRHVGVYGSIYAIADAAFCFAFALGPFFSGPLVKSLGFPTMMYIIAVISFLYAPLMFLLKN 500
[Close]
BLAST of SMED30027753 vs. Ensembl Celegans
Match: cat-1 (pep chromosome:WBcel235:X:5705719:5710585:-1 gene:WBGene00000295.1 transcript:W01C8.6b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:cat-1)

HSP 1 Score: 63.1586 bits (152), Expect = 4.588e-10
Identity = 43/191 (22.51%), Postives = 89/191 (46.60%), Query Frame = -3 
Query:  799 SIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1371
            SI ++ K P++++    + I N  +  L  +LP WM     A  +++G  +    I Y+ G  +         +++  +  I  ++ G  L+ +P +     + IP   + F +G+++  +FPL  +L D      YG++ ++   +       G   SGP+ +  GF  +   + +++ ++  L FLLKN
Sbjct:  249 SIKQLAKDPYIIVAAGAITIGNLGIAMLEPSLPLWMMESWGANSLERGAAFLPASISYLIGTNIFGPLAHRIGRWL--SSFIGLVVIGFSLLSIPSATSVAGLIIPHALLGFSIGMIDASMFPLMGYLVDIRHVGVYGSIYAIADAAFCFAFALGPFFSGPLVKSLGFPTMMYIIAVISFLYAPLMFLLKN 437
[Close]
BLAST of SMED30027753 vs. Ensembl Fly
Match: VAChT (gene:FBgn0270928 transcript:FBtr0083671)

HSP 1 Score: 75.0998 bits (183), Expect = 1.042e-13
Identity = 45/199 (22.61%), Postives = 93/199 (46.73%), Query Frame = -3 
Query:  799 KPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1395
            K  +  +I I R+L  P++ +C   L ++N ++  L  T+  WM   +     + G+VW   F  +V GV +     +  P+  +        + G    ++PF   ++++ +PI  + F + +++  + P   +L D  + S YG++ ++   S +I    G + +G + E  GF  L+  +   NL +  +   L+N
Sbjct:  247 KDVQDQVIPIWRLLMDPYIAVCAGALTMSNVALAFLEPTISLWMEDNMTTDNWKIGMVWLPAFFPHVLGVVITVKMARKYPQHQWLMAAGGLALEGFSCFIIPFCSGYKMLMLPICVICFGIALIDTALLPTLGYLVDVRYVSVYGSIYAIADISYSIAYAVGPIIAGGVVEAIGFTALNFLIAFSNLAYVPVLRKLRN 445
[Close]
BLAST of SMED30027753 vs. Ensembl Fly
Match: Vmat (gene:FBgn0260964 transcript:FBtr0310062)

HSP 1 Score: 66.6254 bits (161), Expect = 5.013e-11
Identity = 50/192 (26.04%), Postives = 89/192 (46.35%), Query Frame = -3 
Query:  799 SIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLV-TLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1371
            S+  ++  P++LI    +  AN  +  L  +LP WM   + AT  +QG+ +    I Y+ G  L   L  K      ++   +  +I G  LI +P +     + IP  G+ F +G+V+  + P   +L D   ++ YG++ +L   +  +    G   SG + +  GF  +   + IL  ++  L  LLKN
Sbjct:  391 SLKSLISDPYILIAAGAITFANMGIAMLEPSLPLWMVDNMGATRWEQGVAFLPASISYLIGTNLFGPLGHKIG---RWFAACLGLIIIGGCLIFIPMATSITHLIIPNAGLGFAIGMVDSSMMPELGYLVDIRHSAVYGSVYALGDVAFCVGFAVGPALSGSLVKSIGFEWMLFGIAILCFMYAPLLTLLKN 579
[Close]
BLAST of SMED30027753 vs. Ensembl Fly
Match: Vmat (gene:FBgn0260964 transcript:FBtr0091491)

HSP 1 Score: 66.6254 bits (161), Expect = 5.013e-11
Identity = 50/192 (26.04%), Postives = 89/192 (46.35%), Query Frame = -3 
Query:  799 SIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLV-TLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1371
            S+  ++  P++LI    +  AN  +  L  +LP WM   + AT  +QG+ +    I Y+ G  L   L  K      ++   +  +I G  LI +P +     + IP  G+ F +G+V+  + P   +L D   ++ YG++ +L   +  +    G   SG + +  GF  +   + IL  ++  L  LLKN
Sbjct:  391 SLKSLISDPYILIAAGAITFANMGIAMLEPSLPLWMVDNMGATRWEQGVAFLPASISYLIGTNLFGPLGHKIG---RWFAACLGLIIIGGCLIFIPMATSITHLIIPNAGLGFAIGMVDSSMMPELGYLVDIRHSAVYGSVYALGDVAFCVGFAVGPALSGSLVKSIGFEWMLFGIAILCFMYAPLLTLLKN 579
[Close]
BLAST of SMED30027753 vs. Ensembl Fly
Match: Vmat (gene:FBgn0260964 transcript:FBtr0091493)

HSP 1 Score: 66.6254 bits (161), Expect = 5.013e-11
Identity = 50/192 (26.04%), Postives = 89/192 (46.35%), Query Frame = -3 
Query:  799 SIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLV-TLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1371
            S+  ++  P++LI    +  AN  +  L  +LP WM   + AT  +QG+ +    I Y+ G  L   L  K      ++   +  +I G  LI +P +     + IP  G+ F +G+V+  + P   +L D   ++ YG++ +L   +  +    G   SG + +  GF  +   + IL  ++  L  LLKN
Sbjct:  391 SLKSLISDPYILIAAGAITFANMGIAMLEPSLPLWMVDNMGATRWEQGVAFLPASISYLIGTNLFGPLGHKIG---RWFAACLGLIIIGGCLIFIPMATSITHLIIPNAGLGFAIGMVDSSMMPELGYLVDIRHSAVYGSVYALGDVAFCVGFAVGPALSGSLVKSIGFEWMLFGIAILCFMYAPLLTLLKN 579
[Close]
BLAST of SMED30027753 vs. Ensembl Fly
Match: Vmat (gene:FBgn0260964 transcript:FBtr0091492)

HSP 1 Score: 66.2402 bits (160), Expect = 6.486e-11
Identity = 50/192 (26.04%), Postives = 89/192 (46.35%), Query Frame = -3 
Query:  799 SIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLV-TLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1371
            S+  ++  P++LI    +  AN  +  L  +LP WM   + AT  +QG+ +    I Y+ G  L   L  K      ++   +  +I G  LI +P +     + IP  G+ F +G+V+  + P   +L D   ++ YG++ +L   +  +    G   SG + +  GF  +   + IL  ++  L  LLKN
Sbjct:  391 SLKSLISDPYILIAAGAITFANMGIAMLEPSLPLWMVDNMGATRWEQGVAFLPASISYLIGTNLFGPLGHKIG---RWFAACLGLIIIGGCLIFIPMATSITHLIIPNAGLGFAIGMVDSSMMPELGYLVDIRHSAVYGSVYALGDVAFCVGFAVGPALSGSLVKSIGFEWMLFGIAILCFMYAPLLTLLKN 579
[Close]
BLAST of SMED30027753 vs. Ensembl Zebrafish
Match: slc18a2 (solute carrier family 18 member 2 [Source:ZFIN;Acc:ZDB-GENE-080514-1])

HSP 1 Score: 85.1149 bits (209), Expect = 9.659e-17
Identity = 51/203 (25.12%), Postives = 101/203 (49.75%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P KV+P      S++ ++K P++LI    +  AN ++  L   LP WM   +C  + Q G+ +    I Y+ G  +  +      +++    +I  ++ GI ++ +P + D   + +P  G+ F +G+V+  + P+  +L D    S YG++ ++   +  +    G  + G IA   GF  L   +G+++++F  L F L+N
Sbjct:  320 PSKVEPESQKGTSLITLMKDPYILIAAGSICFANMAIAMLEPALPIWMMETMCPRKWQLGIAFVPASISYLIGTNIFAVLAHKMGRWL--CSLIGMLLVGISILCVPLAKDIYGLIVPNFGVGFAIGMVDSSMMPIMGYLVDLRHVSVYGSVYAIADVAFCMGFALGPSAGGAIARSIGFPWLMTIIGLVDIMFAPLCFFLRN 520
[Close]
BLAST of SMED30027753 vs. Ensembl Zebrafish
Match: SLC18A1 (solute carrier family 18 member A1 [Source:NCBI gene;Acc:571483])

HSP 1 Score: 84.3445 bits (207), Expect = 1.687e-16
Identity = 55/203 (27.09%), Postives = 99/203 (48.77%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P K+ P       +  +LK P++LI    L  AN  +  L  TLP WM   +CA + Q G+ +    + Y+ G  L  +      +++    ++   I GI L+ +PF+ +   +  P  G+ F VG+V+  +F +  +L D    S YG++ ++   ++ +    G  + G +    GF  L + +GI+N+++  L F LKN
Sbjct:  268 PSKISPTSMEGTPLFILLKDPYILISAGSLCFANMGVAVLEPTLPIWMMQTMCAPKWQLGVAFLPASVSYLIGTNLFGILANKMGRWL--CSMVGMFIVGISLLCVPFAKNLYGLIGPNGGLGFAVGMVDTSMFAIMGYLVDIRHVSVYGSVYAIADVALCVGFAVGPSTGGALVRTIGFPNLMVIIGIINILYAPLCFFLKN 468
[Close]
BLAST of SMED30027753 vs. Ensembl Zebrafish
Match: SLC18A1 (solute carrier family 18 member A1 [Source:NCBI gene;Acc:571483])

HSP 1 Score: 84.3445 bits (207), Expect = 1.707e-16
Identity = 55/203 (27.09%), Postives = 99/203 (48.77%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P K+ P       +  +LK P++LI    L  AN  +  L  TLP WM   +CA + Q G+ +    + Y+ G  L  +      +++    ++   I GI L+ +PF+ +   +  P  G+ F VG+V+  +F +  +L D    S YG++ ++   ++ +    G  + G +    GF  L + +GI+N+++  L F LKN
Sbjct:  269 PSKISPTSMEGTPLFILLKDPYILISAGSLCFANMGVAVLEPTLPIWMMQTMCAPKWQLGVAFLPASVSYLIGTNLFGILANKMGRWL--CSMVGMFIVGISLLCVPFAKNLYGLIGPNGGLGFAVGMVDTSMFAIMGYLVDIRHVSVYGSVYAIADVALCVGFAVGPSTGGALVRTIGFPNLMVIIGIINILYAPLCFFLKN 469
[Close]
BLAST of SMED30027753 vs. Ensembl Zebrafish
Match: SLC18A1 (solute carrier family 18 member A1 [Source:NCBI gene;Acc:571483])

HSP 1 Score: 83.9593 bits (206), Expect = 2.113e-16
Identity = 55/203 (27.09%), Postives = 99/203 (48.77%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P K+ P       +  +LK P++LI    L  AN  +  L  TLP WM   +CA + Q G+ +    + Y+ G  L  +      +++    ++   I GI L+ +PF+ +   +  P  G+ F VG+V+  +F +  +L D    S YG++ ++   ++ +    G  + G +    GF  L + +GI+N+++  L F LKN
Sbjct:  262 PSKISPTSMEGTPLFILLKDPYILISAGSLCFANMGVAVLEPTLPIWMMQTMCAPKWQLGVAFLPASVSYLIGTNLFGILANKMGRWL--CSMVGMFIVGISLLCVPFAKNLYGLIGPNGGLGFAVGMVDTSMFAIMGYLVDIRHVSVYGSVYAIADVALCVGFAVGPSTGGALVRTIGFPNLMVIIGIINILYAPLCFFLKN 462
[Close]
BLAST of SMED30027753 vs. Ensembl Zebrafish
Match: SLC18A1 (solute carrier family 18 member A1 [Source:NCBI gene;Acc:571483])

HSP 1 Score: 83.9593 bits (206), Expect = 2.572e-16
Identity = 55/203 (27.09%), Postives = 99/203 (48.77%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P K+ P       +  +LK P++LI    L  AN  +  L  TLP WM   +CA + Q G+ +    + Y+ G  L  +      +++    ++   I GI L+ +PF+ +   +  P  G+ F VG+V+  +F +  +L D    S YG++ ++   ++ +    G  + G +    GF  L + +GI+N+++  L F LKN
Sbjct:  285 PSKISPTSMEGTPLFILLKDPYILISAGSLCFANMGVAVLEPTLPIWMMQTMCAPKWQLGVAFLPASVSYLIGTNLFGILANKMGRWL--CSMVGMFIVGISLLCVPFAKNLYGLIGPNGGLGFAVGMVDTSMFAIMGYLVDIRHVSVYGSVYAIADVALCVGFAVGPSTGGALVRTIGFPNLMVIIGIINILYAPLCFFLKN 485
[Close]
BLAST of SMED30027753 vs. Ensembl Xenopus
Match: ddx42 (DEAD-box helicase 42 [Source:Xenbase;Acc:XB-GENE-972670])

HSP 1 Score: 78.1814 bits (191), Expect = 1.249e-14
Identity = 55/203 (27.09%), Postives = 94/203 (46.31%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P +      P      +L  P++L+  + L I N +   L  TLP  M   +CA   Q GL +    + Y   + +     +   +++    ++  +I GI +I +P + +   +  P   +    G++E  + PL  HL D    S+YG + +++  ++ I    G L  G IA+  GF  L I LGI+NL+F  L  LL+N
Sbjct:  212 PTRFSTVDVPATPYKNLLMDPYILVAAVGLCICNLTFGMLETTLPIRMMETMCAPRYQLGLCFLPCIVAYFICLNVFAELAQKIGRWLCI--MLGMLILGISVICMPLALNIYYLIGPSAALGIGFGMMETSVMPLMAHLVDLRHTSNYGGIYAISDIALCIGYALGPLCGGAIAKAVGFKWLMIILGIINLVFAPLFILLRN 412
[Close]
BLAST of SMED30027753 vs. Ensembl Xenopus
Match: ddx42 (DEAD-box helicase 42 [Source:Xenbase;Acc:XB-GENE-972670])

HSP 1 Score: 78.5666 bits (192), Expect = 1.333e-14
Identity = 55/203 (27.09%), Postives = 94/203 (46.31%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P +      P      +L  P++L+  + L I N +   L  TLP  M   +CA   Q GL +    + Y   + +     +   +++    ++  +I GI +I +P + +   +  P   +    G++E  + PL  HL D    S+YG + +++  ++ I    G L  G IA+  GF  L I LGI+NL+F  L  LL+N
Sbjct:  260 PTRFSTVDVPATPYKNLLMDPYILVAAVGLCICNLTFGMLETTLPIRMMETMCAPRYQLGLCFLPCIVAYFICLNVFAELAQKIGRWLCI--MLGMLILGISVICMPLALNIYYLIGPSAALGIGFGMMETSVMPLMAHLVDLRHTSNYGGIYAISDIALCIGYALGPLCGGAIAKAVGFKWLMIILGIINLVFAPLFILLRN 460
[Close]
BLAST of SMED30027753 vs. Ensembl Xenopus
Match: ddx42 (DEAD-box helicase 42 [Source:Xenbase;Acc:XB-GENE-972670])

HSP 1 Score: 77.7962 bits (190), Expect = 2.545e-14
Identity = 58/204 (28.43%), Postives = 95/204 (46.57%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPI-ISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P K  P   P      +L  P++++  + L I NFS   +  T+P  M   +C+   Q GL +    + Y   + L      +A K   +  I I  M  GI ++ LP + +   +  P  G+    G+ E  +FP+  HL D    S+YG + +++  ++ I    G    G IA+  GF  L + LGI+NL++  L  LL+N
Sbjct:  283 PTKFSPLSVPETPYRVLLTDPYIVVAAVGLCICNFSFGIMEPTIPIRMMETMCSPPYQLGLAFLPCMLAYFICLNLFA---GFAHKIGRWLCIMIGMMALGISVLCLPLARNIFGLIAPQAGVGIGFGLFETSMFPMMAHLVDIRHTSNYGAIYAISDIALCIGYALGPSCGGAIAKAIGFTWLMVILGIINLVYAPLFILLRN 483
[Close]
BLAST of SMED30027753 vs. Ensembl Xenopus
Match: ddx42 (DEAD-box helicase 42 [Source:Xenbase;Acc:XB-GENE-972670])

HSP 1 Score: 74.7146 bits (182), Expect = 2.461e-13
Identity = 54/188 (28.72%), Postives = 91/188 (48.40%), Query Frame = -3 
Query:  799 MLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPI-ISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1359
            +L  P++++  + L I NFS   +  T+P  M   +C+   Q GL +    + Y   + L      +A K   +  I I  M  GI ++ LP + +   +  P  G+    G+ E  +FP+  HL D    S+YG + +++  ++ I    G    G IA+  GF  L + LGI+NL++  L  LL+N
Sbjct:  266 LLTDPYIVVAAVGLCICNFSFGIMEPTIPIRMMETMCSPPYQLGLAFLPCMLAYFICLNLFA---GFAHKIGRWLCIMIGMMALGISVLCLPLARNIFGLIAPQAGVGIGFGLFETSMFPMMAHLVDIRHTSNYGAIYAISDIALCIGYALGPSCGGAIAKAIGFTWLMVILGIINLVYAPLFILLRN 450
[Close]
BLAST of SMED30027753 vs. Ensembl Xenopus
Match: gtf2e1.2 (general transcription factor IIE, polypeptide 1, alpha 56kDa, gene 2 [Source:Xenbase;Acc:XB-GENE-6001963])

HSP 1 Score: 74.3294 bits (181), Expect = 3.301e-13
Identity = 49/203 (24.14%), Postives = 97/203 (47.78%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P K  P  +       +L+ P++L+    L+IAN ++  L  TLP WM   +C+ + Q G+ +    + Y+    L  +      +++    ++  ++ G  L+ +P + +   +  P   +   +G+V+  I P+  +L D    S YG + ++   ++ +    G   +G IA   GF  L + +GI+N++F  L  LL+N
Sbjct:  231 PSKFSPTTAAPTPYHELLRDPYILVAAACLWIANMAVGMLEPTLPIWMMETMCSPDWQLGIAFLPASVAYLICTNLFGILSHKMGRWL--CSMLGMIVVGGGLLCVPLARNIYGLIGPNAAIGIALGMVDSSIMPIMGYLVDLRHTSVYGGIYAICDTALCLGFAVGPSLAGVIASNIGFSWLMVIIGIINILFSPLCILLRN 431
[Close]
BLAST of SMED30027753 vs. Ensembl Mouse
Match: Slc18a1 (solute carrier family 18 (vesicular monoamine), member 1 [Source:MGI Symbol;Acc:MGI:106684])

HSP 1 Score: 84.3445 bits (207), Expect = 1.872e-16
Identity = 55/213 (25.82%), Postives = 102/213 (47.89%), Query Frame = -3 
Query:  799 DKDSDFC---PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1428
            D    FC   P KV P  +    ++ +LK P++L+    + +AN  +  L  TLP WM   +C+ E Q GL +    + Y+ G  L  +      +++    ++  +  GI L+ +P +H+   +  P  G+ F +G+V+  + P+  +L D    S YG++ ++   +  +    G  + G I    GF  L + +G +N+I+  L   L+N
Sbjct:  267 DGALQFCILWPSKVSPESAMGTPLLTLLKDPYILVAAGSICLANMGVAILEPTLPIWMMQTMCSPEWQLGLAFLPASVAYLIGTNLFGVLANKMGRWL--CSLVGMVAVGISLLCVPLAHNIFGLIGPNAGLGFAIGMVDSSLMPIMGYLVDLRHTSVYGSVYAIADVAFCVGFAIGPSTGGVIVPVIGFPWLMVIIGTINIIYAPLCCFLQN 477
[Close]
BLAST of SMED30027753 vs. Ensembl Mouse
Match: Slc18a2 (solute carrier family 18 (vesicular monoamine), member 2 [Source:MGI Symbol;Acc:MGI:106677])

HSP 1 Score: 80.8777 bits (198), Expect = 2.254e-15
Identity = 49/203 (24.14%), Postives = 100/203 (49.26%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P +V+P       +  +LK P++LI    +  AN  +  L   LP WM   +C+ + Q G+ +    I Y+ G  +  +      +++    ++  ++ GI ++ +PF+ +   +  P  G+ F +G+V+  + P+  +L D    S YG++ ++   +  +    G  + G IA+  GF  L   +GI++++F  L F L++
Sbjct:  275 PSRVQPESQKGTPLTTLLKDPYILIAAGSICFANMGIAMLEPALPIWMMETMCSRKWQLGVAFLPASISYLIGTNIFGILAHKMGRWL--CALLGMIVVGISILCIPFAKNIYGLIAPNFGVGFAIGMVDSSMMPIMGYLVDLRHVSVYGSVYAIADVAFCMGYAIGPSAGGAIAKAIGFPWLMTIIGIIDIVFAPLCFFLRS 475
[Close]
BLAST of SMED30027753 vs. Ensembl Mouse
Match: Slc18a3 (solute carrier family 18 (vesicular monoamine), member 3 [Source:MGI Symbol;Acc:MGI:1101061])

HSP 1 Score: 70.8626 bits (172), Expect = 3.085e-12
Identity = 44/179 (24.58%), Postives = 85/179 (47.49%), Query Frame = -3 
Query:  835 IMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGL-VTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILN 1368
            I R++  P++ +    L   N  +  L  T+ +WM   + A+E + G+VW   F+ +V GV L V L  +Y P   +    +   + G+   ++P    +  + + + G+ F + +V+  + P    L D    S YG++ ++   S ++    G + +G I    GF  LS+ +G+ N
Sbjct:  282 IHRLMLDPYIAVVAGALTTCNIPLAFLEPTIATWMKHTMAASEWEMGMVWLPAFVPHVLGVYLTVRLAARY-PHLQWLYGALGLAVIGVSSCVVPACRSFAPLVVSLCGLCFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYSVAYALGPIVAGHIVHSLGFEQLSLGMGLAN 459
[Close]
BLAST of SMED30027753 vs. UniProt/SwissProt
Match: sp|Q01818|VMAT1_RAT (Chromaffin granule amine transporter OS=Rattus norvegicus OX=10116 GN=Slc18a1 PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 4.325e-16
Identity = 53/203 (26.11%), Postives = 101/203 (49.75%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P KV P  +   S++ +LK P++L+    + +AN  +  L  TLP WM   +C+ E Q GL +    + Y+ G  L  +      +++    ++  +  GI L+ +P +H+   +  P  G+ F +G+V+  + P+  +L D    S YG++ ++   +  +    G  + G I +  GF  L + +G +N+I+  L   L+N
Sbjct:  277 PSKVSPESAMGTSLLTLLKDPYILVAAGSICLANMGVAILEPTLPIWMMQTMCSPEWQLGLAFLPASVAYLIGTNLFGVLANKMGRWL--CSLVGMVAVGISLLCVPLAHNIFGLIGPNAGLGFAIGMVDSSLMPIMGYLVDLRHTSVYGSVYAIADVAFCVGFAIGPSTGGVIVQVIGFPWLMVIIGTINIIYAPLCCFLQN 477
[Close]
BLAST of SMED30027753 vs. UniProt/SwissProt
Match: sp|Q8R090|VMAT1_MOUSE (Chromaffin granule amine transporter OS=Mus musculus OX=10090 GN=Slc18a1 PE=1 SV=1)

HSP 1 Score: 84.3445 bits (207), Expect = 1.310e-15
Identity = 55/213 (25.82%), Postives = 102/213 (47.89%), Query Frame = -3 
Query:  799 DKDSDFC---PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1428
            D    FC   P KV P  +    ++ +LK P++L+    + +AN  +  L  TLP WM   +C+ E Q GL +    + Y+ G  L  +      +++    ++  +  GI L+ +P +H+   +  P  G+ F +G+V+  + P+  +L D    S YG++ ++   +  +    G  + G I    GF  L + +G +N+I+  L   L+N
Sbjct:  267 DGALQFCILWPSKVSPESAMGTPLLTLLKDPYILVAAGSICLANMGVAILEPTLPIWMMQTMCSPEWQLGLAFLPASVAYLIGTNLFGVLANKMGRWL--CSLVGMVAVGISLLCVPLAHNIFGLIGPNAGLGFAIGMVDSSLMPIMGYLVDLRHTSVYGSVYAIADVAFCVGFAIGPSTGGVIVPVIGFPWLMVIIGTINIIYAPLCCFLQN 477
[Close]
BLAST of SMED30027753 vs. UniProt/SwissProt
Match: sp|P54219|VMAT1_HUMAN (Chromaffin granule amine transporter OS=Homo sapiens OX=9606 GN=SLC18A1 PE=1 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 6.063e-15
Identity = 49/203 (24.14%), Postives = 98/203 (48.28%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P KV P  +    +  +LK P++L+    +  AN  +  L  TLP WM   +C+ + Q GL +    + Y+ G  L  +      +++    +I  ++ G  L+ +P +H+   +  P  G+   +G+V+  + P+  HL D    S YG++ ++   +  +    G  + G I +  GF  L +  G++N+++  L + L++
Sbjct:  280 PSKVSPESAKGTPLFMLLKDPYILVAAGSICFANMGVAILEPTLPIWMMQTMCSPKWQLGLAFLPASVSYLIGTNLFGVLANKMGRWL--CSLIGMLVVGTSLLCVPLAHNIFGLIGPNAGLGLAIGMVDSSMMPIMGHLVDLRHTSVYGSVYAIADVAFCMGFAIGPSTGGAIVKAIGFPWLMVITGVINIVYAPLCYYLRS 480
[Close]
BLAST of SMED30027753 vs. UniProt/SwissProt
Match: sp|Q8BRU6|VMAT2_MOUSE (Synaptic vesicular amine transporter OS=Mus musculus OX=10090 GN=Slc18a2 PE=1 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 1.576e-14
Identity = 49/203 (24.14%), Postives = 100/203 (49.26%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P +V+P       +  +LK P++LI    +  AN  +  L   LP WM   +C+ + Q G+ +    I Y+ G  +  +      +++    ++  ++ GI ++ +PF+ +   +  P  G+ F +G+V+  + P+  +L D    S YG++ ++   +  +    G  + G IA+  GF  L   +GI++++F  L F L++
Sbjct:  275 PSRVQPESQKGTPLTTLLKDPYILIAAGSICFANMGIAMLEPALPIWMMETMCSRKWQLGVAFLPASISYLIGTNIFGILAHKMGRWL--CALLGMIVVGISILCIPFAKNIYGLIAPNFGVGFAIGMVDSSMMPIMGYLVDLRHVSVYGSVYAIADVAFCMGYAIGPSAGGAIAKAIGFPWLMTIIGIIDIVFAPLCFFLRS 475
[Close]
BLAST of SMED30027753 vs. UniProt/SwissProt
Match: sp|Q05940|VMAT2_HUMAN (Synaptic vesicular amine transporter OS=Homo sapiens OX=9606 GN=SLC18A2 PE=1 SV=2)

HSP 1 Score: 80.1073 bits (196), Expect = 2.378e-14
Identity = 49/203 (24.14%), Postives = 100/203 (49.26%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P +V+P       +  +LK P++LI    +  AN  +  L   LP WM   +C+ + Q G+ +    I Y+ G  +  +      +++    ++  +I G+ ++ +PF+ +   +  P  G+ F +G+V+  + P+  +L D    S YG++ ++   +  +    G  + G IA+  GF  L   +GI++++F  L F L++
Sbjct:  272 PSRVQPESQKGTPLTTLLKDPYILIAAGSICFANMGIAMLEPALPIWMMETMCSRKWQLGVAFLPASISYLIGTNIFGILAHKMGRWL--CALLGMIIVGVSILCIPFAKNIYGLIAPNFGVGFAIGMVDSSMMPIMGYLVDLRHVSVYGSVYAIADVAFCMGYAIGPSAGGAIAKAIGFPWLMTIIGIIDILFAPLCFFLRS 472
[Close]
BLAST of SMED30027753 vs. TrEMBL
Match: A0A0H3YJ27 (Slc18a-1 OS=Schmidtea mediterranea OX=79327 GN=slc18a-1 PE=2 SV=1)

HSP 1 Score: 324.324 bits (830), Expect = 1.766e-120
Identity = 182/315 (57.78%), Postives = 222/315 (70.48%), Query Frame = -3 
Query:  841 FSDINTRGQYFGLGNTLYFFSHSIGAVYGAEIDSRLGKSPVFF-------FXXXXXXXXXXXXXXLCKNVSNGFQNDDKNEAGIKTIYNKDIIETNNESEGNKKEIQKQVINSDQSPDKDSDFCPMKV--KPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGI 1758
            FS++NTRG++FGLGNTLYF SHSIG VYGAE+D RLGKS VFF       F +    +    T   C    N F  ++K   G+         ETN   + N+ ++   + ++ + P+K SDF   K   K N    +   +++K P+V  CL QLFI N SMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGL+ L  K A +  F++PII+Q+I GILLILLP SHDWRL+FIPIIGMYF VG++EQV+FPLTT+LAD  FNSDYG+M+SLTSQS+T+  V GTLSSGPIA+RFGF  L I LGI
Sbjct:  150 FSNVNTRGRFFGLGNTLYFVSHSIGPVYGAEVDVRLGKSAVFFLLIPLWIFTMAATFILYRRTEKRCNKSKNNF--NEKLSIGVTPD------ETNELDDHNELKVAIIISSNSKMPEKYSDFKGEKKFQKENNEIFMKFYKIMKNPYVSSCLCQLFIVNLSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLIVLLLKCAYQLRFFSPIIAQIIVGILLILLPLSHDWRLLFIPIIGMYFSVGVIEQVLFPLTTYLADHHFNSDYGSMASLTSQSLTLSGVVGTLSSGPIAQRFGFRSLCICLGI 456

HSP 2 Score: 140.584 bits (353), Expect = 1.766e-120
Identity = 78/162 (48.15%), Postives = 104/162 (64.20%), Query Frame = -2 
Query: 1757 MQFNCGSVLVILMVSLNSFMITIPESTLYTILPKYFVDKHRSSXXXXXXXXXXXXXXXXNLRINISKL---CPLKKPPYFMVSMVFLAKNLAQVISNSIVGCIVDKVNNFTILITAQCFGIVVTLIYGFGNSVIAYMCGRVLDAFVNSSLFISVMAIISNLF 2233
            M F C   L  +++ LN +M+ I ES+LYTILPKY VD+  ++T N               + N S+    C L+KPPYF+VSM+FL+K+LAQ   NSI+GC VDKVNNF IL+  Q  GI++ LIY   NS+  Y+ GR+LDAFV+ SLFI   +I+S LF
Sbjct:    1 MSFKCDDCLAAIVICLNFYMLGIQESSLYTILPKYLVDEPFNATNNET------------FKPNTSRTIFQCHLQKPPYFIVSMIFLSKSLAQFFFNSIIGCFVDKVNNFIILLIGQGLGIIIALIYALNNSITMYIVGRLLDAFVSCSLFIGGFSIVSKLF 150
[Close]
BLAST of SMED30027753 vs. TrEMBL
Match: A0A0H3YK88 (Slc18a-8 OS=Schmidtea mediterranea OX=79327 GN=slc18a-8 PE=2 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 7.257e-79
Identity = 140/313 (44.73%), Postives = 197/313 (62.94%), Query Frame = -3 
Query:  826 FSDINTRGQYFGLGNTLYFFSHSIGAVYGAEIDSRLGKSPVFFFXXXXXXXXXXXXXXLCKNVSNGFQNDDKNEAGIKTIYNKDIIETNNE--SEGNKKEIQKQVINSDQSPDKDSDFCPMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIF 1758
            F  I  R +Y+ L N +YF SHSIG  YGAE+D R GKS VF  L+PLWIV +L+T+ L     N      ++E  ++    K  +E+ +E  +E N + + K          K   F     K  K+ L  I + ++ PFV++CL QLF+ N SM++LP TLPSWM    C++EI+QG+VW FGF+GY+ GV  V +  K+A +F +Y P++  ++ GIL+I+LPFS DW++VF+PI+GMY  VG V+Q++ PLT +LA     S+   +SSLTSQS+TI  V  TL  G I   FGFH L+I+LGI+NLIF
Sbjct:  149 FIVIEVRDRYYALVNIIYFISHSIGPFYGAEVDERFGKSTVFLILVPLWIVCLLLTVFLYWRNRN---RSKRSEIKLEGQRQKPNLESLDECINEANVQILDKA---------KMDIFVQNSRKKTKT-LNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYIVGVVTVIIVDKHAYRFRYYVPVVGHLMIGILMIILPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNIVSSLTSQSLTISSVISTLLCGHIVMLFGFHFLTITLGIINLIF 448

HSP 2 Score: 96.2857 bits (238), Expect = 7.257e-79
Identity = 56/159 (35.22%), Postives = 94/159 (59.12%), Query Frame = -2 
Query: 1757 MQFNCGSVLVILMVSLNSFMITIPESTLYTILPKYFVDKHRSSXXXXXXXXXXXXXXXXNLRINISKLCPLKKPPYFMVSMVFLAKNLAQVISNSIVGCIVDKVNNFTILITAQCFGIVVTLIYGFGNSVIAYMCGRVLDAFVNSSLFISVMAIISNLF 2233
            M F   ++L  ++VSLN F++ + E++LY ILPKYF ++   S  N   +          +   + + C  +KPPY +VS +FL+KN+A +  N  +G I+ K+N + ILI +   GI V+ IY + ++++ Y+ GRVLD+F+   + I +  +IS LF
Sbjct:    1 MFFKFDNLLAGVVVSLNFFLMVLQENSLYAILPKYFSNQTSISARNETVL----------INSTVPQNCSFQKPPYVVVSQIFLSKNVAMIFLNIFIGIIIYKLNIYIILILSHFVGIGVSFIYAYTDTLLLYIIGRVLDSFITCGILIGIYFLISKLF 149
[Close]
BLAST of SMED30027753 vs. TrEMBL
Match: A0A2I6QBP9 (Vesicular acetylcholine transporter (Fragment) OS=Terebratalia transversa OX=34513 PE=2 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 5.247e-19
Identity = 64/216 (29.63%), Postives = 110/216 (50.93%), Query Frame = -3 
Query:  802 INSDQSPDKDSDFCPMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLK 1449
            I  ++   K +   P+   P       I R+L  P++ I    L ++N S+  L  TL +WM   + + E Q GLVW   FI +V GV L     K  PK  +    I  M+ G++ +++PFS ++ +V IPI+G+ F + +V+  + P   +L D    S YG++ ++   S ++   FG + +G +    GF  L+I + + N+I+  L  +LK
Sbjct:  262 IREERRLKKANSEVPLNGTP-------IWRLLMDPYIAITAGALAMSNVSLAFLEPTLANWMESTMGSKEWQTGLVWLPAFIPHVLGVVLTVKLAKAYPKHQWLLAFIGLMMEGLMCLIIPFSGEFGVVIIPIMGICFGIALVDTALLPTLGYLVDVRHVSVYGSVYAIADISYSMAYAFGPIIAGSVVAAIGFLWLNIIICLTNVIYAPLLIILK 470
[Close]
BLAST of SMED30027753 vs. TrEMBL
Match: A0A5N5STS3 (Vesicular acetylcholine transporter OS=Armadillidium nasatum OX=96803 GN=VAChT PE=4 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 2.347e-18
Identity = 57/211 (27.01%), Postives = 104/211 (49.29%), Query Frame = -3 
Query:  799 PDKDSDFCPMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1431
            P KD         P  +P   I R+   P++L+C   L ++N S+  L  T+  WM   +   E Q G++W   F  +V+GV L        PK+ +   +I   + GI    LPF+ ++ +V +PI  + F + +++  + P   +L D  + S YG++ ++   S +    FG + +G +    GF  L+I + + NL++C L + L++
Sbjct:  266 PIKDEQRDKGYEHPKGTP---IFRLFMDPYILVCAGALMMSNVSLAFLEPTISVWMMDNMHVKEWQLGMIWLPSFFPHVAGVILTVKLSNLYPKYQWVMAVIGLALEGISCFFLPFATNYWVVMLPICVICFGIALIDTALLPTLGYLVDTRYVSVYGSIYAIADISYSFAYAFGPMIAGSVVASIGFVALNIGIALSNLLYCPLLYSLRH 473
[Close]
BLAST of SMED30027753 vs. TrEMBL
Match: A0A444T4C9 (Vesicular acetylcholine transporter OS=Armadillidium vulgare OX=13347 GN=Avbf_11944 PE=4 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 3.241e-18
Identity = 56/211 (26.54%), Postives = 105/211 (49.76%), Query Frame = -3 
Query:  799 PDKDSDFCPMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1431
            P KD        +P  +P   I R+   P++L+C   L ++N S+  L  T+  WM   +   E Q G++W   F  +V+GV L        PK+ +   ++   + GI    LPF+ ++ +V +PI  + F + +++  + P   +L D  + S YG++ ++   S +    FG + +G +    GF  L+I + + NL++C L + L++
Sbjct:  266 PIKDERRDKGYEQPKGTP---IFRLFMDPYILVCAGALMMSNVSLAFLEPTISVWMMDNMHVKEWQLGMIWLPSFFPHVAGVILTVKLSNLYPKYQWVMAVVGLALEGISCFFLPFATNYWVVMLPICFICFGIALIDTALLPTLGYLVDTRYVSVYGSIYAIADISYSFAYAFGPMIAGSVVSSIGFVALNIGIALSNLLYCPLLYSLRH 473
[Close]
BLAST of SMED30027753 vs. Ensembl Cavefish
Match: slc18a2 (synaptic vesicular amine transporter [Source:NCBI gene;Acc:103032219])

HSP 1 Score: 90.5077 bits (223), Expect = 1.484e-18
Identity = 54/203 (26.60%), Postives = 104/203 (51.23%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P KV+P      S+M ++K P++LI    +  AN ++  L   LP WM   +CA + Q G+ +    + Y+ G  +  +      +++    +I  ++ GI ++ +PF+ D   + +P  G+ F +G+V+  + P+  +L D    S YG++ ++   +  +   FG  + G IA   GF  L   +GI++++F  L F L++
Sbjct:  270 PSKVEPESQKGTSLMTLMKDPYILIAAGSICFANMAIAMLEPALPIWMMETMCARKWQLGVAFLPASVSYLIGTNIFAVLANKMGRWL--CALIGMLLVGISILCVPFAKDIYGLILPNFGVGFAIGMVDSSMMPIMGYLVDLRHISVYGSVYAIADVAFCMGFAFGPSAGGAIARSIGFPWLMTIIGIVDILFAPLCFFLRS 470
[Close]
BLAST of SMED30027753 vs. Ensembl Cavefish
Match: SLC18A1 (solute carrier family 18 member A1 [Source:NCBI gene;Acc:103028146])

HSP 1 Score: 82.0333 bits (201), Expect = 7.306e-16
Identity = 54/203 (26.60%), Postives = 99/203 (48.77%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P K+ P       ++ +LK P++LI    L  AN  +  L  TLP WM   +C+ + Q G+ +    + Y+ G  L  L      +++    +I   I GI LI +P + +   +  P  G+ F +G+V+  +  +  +L D   +S YG++ ++   ++ +    G  + G I    GF  L + +GI+N+++  L + LKN
Sbjct:  268 PSKISPGSVEGTPLLTLLKDPYILISAGSLCFANMGVAILEPTLPIWMMQTMCSQKWQLGVAFLPASVSYLIGTNLFGLLANKMGRWL--CSMIGMFIVGISLICVPLASNIYGLIGPNGGLGFAIGMVDSSMMAIMGYLVDIRHSSVYGSVYAIADVALCMGFAIGPSTGGAIVRVIGFPYLMVMIGIVNILYAPLCYFLKN 468
[Close]
BLAST of SMED30027753 vs. Ensembl Cavefish
Match: SLC18A1 (solute carrier family 18 member A1 [Source:NCBI gene;Acc:103028146])

HSP 1 Score: 81.6481 bits (200), Expect = 8.424e-16
Identity = 54/203 (26.60%), Postives = 99/203 (48.77%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P K+ P       ++ +LK P++LI    L  AN  +  L  TLP WM   +C+ + Q G+ +    + Y+ G  L  L      +++    +I   I GI LI +P + +   +  P  G+ F +G+V+  +  +  +L D   +S YG++ ++   ++ +    G  + G I    GF  L + +GI+N+++  L + LKN
Sbjct:  276 PSKISPGSVEGTPLLTLLKDPYILISAGSLCFANMGVAILEPTLPIWMMQTMCSQKWQLGVAFLPASVSYLIGTNLFGLLANKMGRWL--CSMIGMFIVGISLICVPLASNIYGLIGPNGGLGFAIGMVDSSMMAIMGYLVDIRHSSVYGSVYAIADVALCMGFAIGPSTGGAIVRVIGFPYLMVMIGIVNILYAPLCYFLKN 476
[Close]
BLAST of SMED30027753 vs. Ensembl Cavefish
Match: slc18a3b (solute carrier family 18 member 3b [Source:NCBI gene;Acc:394082])

HSP 1 Score: 77.0258 bits (188), Expect = 2.428e-14
Identity = 44/199 (22.11%), Postives = 94/199 (47.24%), Query Frame = -3 
Query:  799 KPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1395
            + N+   + + R++  P++ +    L + N  +  L  T+  WM   + AT+ Q GL W   F  +V GV L     +  P   ++   +  +I G    ++P    +  + +P+ G+ F + +V+  + P    L D  + S YG++ ++   S ++    G +++G I    GF  L++ +G+ N+++      L++
Sbjct:  216 RQNQPAGVPLHRLIMDPYIAVVAGALTVCNIPLAFLEPTIAGWMENTMHATKWQIGLAWLPSFFPHVLGVYLTVKLAERYPSMQWFYGAVGMLIIGASSCVVPACKSFSQLMLPLCGICFGIALVDTALLPTMAFLVDVRYASVYGSVYAIADISYSVAYALGPVAAGHIVHTMGFAQLNLGMGLANVLYAPALLALRH 414
[Close]
BLAST of SMED30027753 vs. Ensembl Cavefish
Match: slc18a3a (solute carrier family 18 member A3 [Source:NCBI gene;Acc:103044616])

HSP 1 Score: 69.707 bits (169), Expect = 5.216e-12
Identity = 46/191 (24.08%), Postives = 91/191 (47.64%), Query Frame = -3 
Query:  799 IMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGL-VTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1368
            I +++  P++ +    L   N  +  L  T+ +WM   + +++ Q GL W   F  +V GV + V L  +Y P   ++   +  +I G     +P   ++  + IP+ G+ F + +V+  + P    L D    S YG++ ++   S  +    G + +G I    GF  L++ +G+ N+++     LL+N
Sbjct:  259 IYKLMIDPYIAVVAGALTTCNIPLAFLEPTIANWMEETMDSSQWQIGLTWLPAFFPHVLGVYITVKLAAQY-PHLQWFYGALGMVIIGASSCTVPACKNFEQLIIPLCGICFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYCVAYAMGPIVAGKIVHDLGFVQLNLGMGLANVLYAPALLLLRN 448
[Close]
BLAST of SMED30027753 vs. Ensembl Sea Lamprey
Match: slc18a3a (solute carrier family 18 member 3a [Source:NCBI gene;Acc:559347])

HSP 1 Score: 83.1889 bits (204), Expect = 7.125e-17
Identity = 54/199 (27.14%), Postives = 100/199 (50.25%), Query Frame = -3 
Query:  799 PNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGL-VTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1392
            P  +P   I R++  P++++    L +AN  +  L  T+ SWM   + A E Q+G+ W   F  +V GV + V L  KY P+  ++   +  +I G    L+P    +  +  P+ G+ F + +V+  + PL   L D    S YG++ ++   S ++    G + +G I    GF  LS+ +G+ N+++  +  LL++
Sbjct:  268 PQGTP---IYRLMMDPYIVVVAGALTMANIPLAFLEPTIASWMKKTMGANEWQRGMTWLPAFFPHVLGVYITVKLAAKY-PQLQWFYGGLGLVIIGSSSCLVPACRTFEELIAPLCGICFGIALVDTALLPLLAFLVDVRHASVYGSVYAIADISYSLAYAMGPIVAGQIVHTMGFVQLSLGMGLTNVLYAPMLLLLRH 462
[Close]
BLAST of SMED30027753 vs. Ensembl Sea Lamprey
Match: slc18a2 (solute carrier family 18 member 2 [Source:ZFIN;Acc:ZDB-GENE-080514-1])

HSP 1 Score: 75.485 bits (184), Expect = 1.613e-14
Identity = 50/203 (24.63%), Postives = 97/203 (47.78%), Query Frame = -3 
Query:  799 PMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1407
            P K +P       ++ +L+ P++LI    + IAN  +  L   LP WM   +C+ + Q G+ +    I Y  G  +         +++    +I  ++ GI +I +P +     + +P  G+ F +G+V+  + PL  +L D    S YG++ ++   +  +    G  + G IA   GF  L   +GI++++F  L + L++
Sbjct:  292 PSKSEPESQTGTPLLTLLRDPYILITAGSICIANMGIAMLEPALPIWMMETMCSPKWQLGVAFIPASISYWIGTNIFGPLAHKMGRWL--CALIGMVVVGISIICVPLAKTIYGLIVPNFGVGFAIGMVDSSMMPLMGYLVDLRHVSVYGSVYAIADVAFCMGFALGPSAGGAIARTIGFPWLMTIIGIIDILFAPLCYFLRS 492
[Close]
BLAST of SMED30027753 vs. Ensembl Medaka
Match: SLC18A1 (solute carrier family 18 member A1 [Source:NCBI gene;Acc:101172172])

HSP 1 Score: 82.8037 bits (203), Expect = 3.167e-16
Identity = 56/209 (26.79%), Postives = 106/209 (50.72%), Query Frame = -3 
Query:  799 KDSDFCPMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1425
            + S  CP  V+   +PL++   +LK P++LI    L  AN  +  L  TLP WM   +C+ + Q G+ +    I Y+ G  L  +      +++    ++  +I GI L+ +PF+     +  P  G+ F +G+V+  +  +  +L D    S YGT+ ++   ++ +    G  + G + +  GF CL + +G++N+++  L  LL+N
Sbjct:  227 QPSKICPGSVE--GTPLLT---LLKDPYILISAGSLCFANMGVAILEPTLPIWMMQTMCSPKWQLGVAFLPASISYLIGTNLFGVLANKMGRWL--CSMLGMVIVGISLLCVPFATSIYGLIGPNGGLGFAIGMVDSSMMAIMGYLVDIRHASVYGTVYAIADVALCMGFAIGPSTGGALVQAVGFPCLMVFIGVINILYAPLCLLLRN 428
[Close]
BLAST of SMED30027753 vs. Ensembl Medaka
Match: SLC18A1 (solute carrier family 18 member A1 [Source:NCBI gene;Acc:101172172])

HSP 1 Score: 82.4185 bits (202), Expect = 5.817e-16
Identity = 56/209 (26.79%), Postives = 106/209 (50.72%), Query Frame = -3 
Query:  799 KDSDFCPMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1425
            + S  CP  V+   +PL++   +LK P++LI    L  AN  +  L  TLP WM   +C+ + Q G+ +    I Y+ G  L  +      +++    ++  +I GI L+ +PF+     +  P  G+ F +G+V+  +  +  +L D    S YGT+ ++   ++ +    G  + G + +  GF CL + +G++N+++  L  LL+N
Sbjct:  294 QPSKICPGSVE--GTPLLT---LLKDPYILISAGSLCFANMGVAILEPTLPIWMMQTMCSPKWQLGVAFLPASISYLIGTNLFGVLANKMGRWL--CSMLGMVIVGISLLCVPFATSIYGLIGPNGGLGFAIGMVDSSMMAIMGYLVDIRHASVYGTVYAIADVALCMGFAIGPSTGGALVQAVGFPCLMVFIGVINILYAPLCLLLRN 495
[Close]
BLAST of SMED30027753 vs. Ensembl Medaka
Match: slc18a3a (solute carrier family 18 member 3a [Source:NCBI gene;Acc:559347])

HSP 1 Score: 75.485 bits (184), Expect = 9.240e-14
Identity = 48/198 (24.24%), Postives = 92/198 (46.46%), Query Frame = -3 
Query:  775 IMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKNSNKL*VSF 1368
            I +++  P++ +    L I N  +  L  T+ +WM   + ATE + G+ W   F  +V GV L        P   ++   +  +  G     +P   ++  + IP+ G+ F + +V+  + P    L D    S YG++ ++   S  +    G + +G I    GF  L++ +G+ N+++     LLKN +++  SF
Sbjct:  264 IYKLMIDPYIAVVAGALIICNIPLAFLEPTIANWMEDTMQATEWEIGMTWFPAFFPHVLGVYLTVKLAAKHPHLQWFYGAMGMVFIGASSCTVPACKNFGQLMIPLCGVCFGIALVDTALLPTLGFLVDVRHVSVYGSVYAIADISYCVAYALGPVVAGQIVHNLGFVQLNLGMGLANVLYAPALLLLKNVSQMKPSF 461
[Close]
BLAST of SMED30027753 vs. Ensembl Medaka
Match: slc18a3b (probable vesicular acetylcholine transporter-B [Source:NCBI gene;Acc:101163117])

HSP 1 Score: 72.7886 bits (177), Expect = 5.473e-13
Identity = 45/209 (21.53%), Postives = 96/209 (45.93%), Query Frame = -3 
Query:  775 KVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKNSNKL*VSF 1401
            + + N      I +++  P++ +    L +AN  +  L  T+ +WM   + +T+ + GL W   F  +V GV +        P   ++   +  +I G     +P    +  +  P+ G+ F + +V+  + P    L D    S YG++ ++   S ++    G + +G I    GF  L++ +G++N+++     LL+  +++  SF
Sbjct:  251 RTRENMPVGTPIYKLMVDPYIAVVAGALTVANIPLAFLEPTIANWMETTMHSTQWEMGLTWLPAFFPHVLGVYITVKLAAKNPHLQWFYGALGMVIIGASSCTVPACKTFGQLIAPLCGICFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYSVAYAMGPIVAGQIVHTLGFVQLNLGMGLVNVLYAPALLLLRTVSQMKPSF 459
[Close]
BLAST of SMED30027753 vs. Ensembl Medaka
Match: slc18a2 (solute carrier family 18 member A2 [Source:NCBI gene;Acc:101158793])

HSP 1 Score: 55.4546 bits (132), Expect = 1.595e-7
Identity = 52/197 (26.40%), Postives = 90/197 (45.69%), Query Frame = -3 
Query:  799 SPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLV-TLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCV-GIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKN 1383
            +PL+++M   K P++LI    +   N ++  +  TLP WM   +C  + Q G+ +    I Y+ G  +  TL  K              + + I ++L+ FS     V   I  +  CV G+V+  + P+  +L D    S YG++ ++   +  +    G    G IAE  GF  L   +GI+++ F  L   L+N
Sbjct:  280 TPLLTLM---KDPYILIAAGAICFGNMAIAMMEPTLPIWMMETMCTRKWQLGIAFLPASISYLIGTNIFGTLAHKMG----------RWLCALIGMVLVGFS-----VICVISHVMSCVAGMVDSSMMPIMGYLVDLRHISVYGSVYAIADVAFCMGFALGPSIGGSIAENIGFPWLMTIIGIVDIFFAPLCIFLRN 458
[Close]
BLAST of SMED30027753 vs. Planmine SMEST
Match: SMESG000041534.1 (SMESG000041534.1)

HSP 1 Score: 589.726 bits (1519), Expect = 0.000e+0
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = -3 
Query:  787 SHSIGAVYGAEIDSRLGKSPVFFFXXXXXXXXXXXXXXLCKNVSNGFQNDDKNEAGIKTIYNKDIIETNNESEGNKKEIQKQVINSDQSPDKDSDFCPMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKNSNKL 1698
            SHSIGAVYGAEIDSRLGKSPVFFFLIPLWIVTILVTILLCKNVSNGFQNDDKNEAGIKTIYNKDIIETNNESEGNKKEIQKQVINSDQSPDKDSDFCPMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKNSNKL
Sbjct:  117 SHSIGAVYGAEIDSRLGKSPVFFFLIPLWIVTILVTILLCKNVSNGFQNDDKNEAGIKTIYNKDIIETNNESEGNKKEIQKQVINSDQSPDKDSDFCPMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKNSNKL 420

HSP 2 Score: 157.918 bits (398), Expect = 9.806e-42
Identity = 102/125 (81.60%), Postives = 105/125 (84.00%), Query Frame = -2 
Query: 1859 MQFNCGSVLVILMVSLNSFMITIPESTLYTILPKYFVDKHRSSXXXXXXXXXXXXXXXXNLRINISKLCPLKKPPYFMVSMVFLAKNLAQVISNSIVGCIVDKVNNFTILITAQCFGIVVTLIYG 2233
            MQFNCGSVLVILMVSLNSFMITIPESTLYTILPKYFVDKHRSSTTNNNTIVDVTVNDTTNLRINISKLCPLKKPPYFMVSMVFLAKNLAQVISNSI   +V   NN       Q     +  +YG
Sbjct:    1 MQFNCGSVLVILMVSLNSFMITIPESTLYTILPKYFVDKHRSSTTNNNTIVDVTVNDTTNLRINISKLCPLKKPPYFMVSMVFLAKNLAQVISNSIRNVLVFHGNNLKSFFRYQHESHSIGAVYG 125
[Close]
BLAST of SMED30027753 vs. Planmine SMEST
Match: SMESG000041535.1 (SMESG000041535.1)

HSP 1 Score: 478.789 bits (1231), Expect = 2.898e-180
Identity = 262/322 (81.37%), Postives = 276/322 (85.71%), Query Frame = -3 
Query:  796 FFSDINTRGQYFGLGNTLYFFSHSIGAVYGAEIDSRLGKSPVFFFXXXXXXXXXXXXXXLCKNVSNGFQNDDKNEAGIKTIYNKDIIETNNESEGNKKEIQKQVINSDQSPDKDSDFCPMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKNS 1761
             FSDINTRG+Y GLGNTLYFFSHSIGAVYGAEID+RLGKSPVFF LIPLWIVTI +TI +   V    Q+  K    IK   +K+ IE  N +  N  E   +VINS Q+PDKDSDF PMK KPNKSPLISIM MLKTP VLICLIQLFI NFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFY PII+QMI GILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQS+TIC V GTLSSGPIAERFGFH LSI+LGILNL FC L FLL+NS
Sbjct:  149 LFSDINTRGRYIGLGNTLYFFSHSIGAVYGAEIDTRLGKSPVFFLLIPLWIVTISITISIYSRV----QDKVKYGHEIKKCDDKETIEIENLNNENTSEYPAEVINSVQNPDKDSDFSPMKAKPNKSPLISIMWMLKTPSVLICLIQLFIVNFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYNPIIAQMIIGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSLTICGVMGTLSSGPIAERFGFHTLSIALGILNLTFCGLHFLLRNS 466

HSP 2 Score: 174.096 bits (440), Expect = 2.898e-180
Identity = 95/150 (63.33%), Postives = 121/150 (80.67%), Query Frame = -2 
Query: 1757 MITIPESTLYTILPKYFVDK----------HRSSXXXXXXXXXXXXXXXXNLRINISKLCPLKKPPYFMVSMVFLAKNLAQVISNSIVGCIVDKVNNFTILITAQCFGIVVTLIYGFGNSVIAYMCGRVLDAFVNSSLFISVMAIISNLF 2176
            M++IPES+LYTILPKY VD+          + +     N   +VT+ND  NLRINISKLC ++KPPYF+VSMVFL+KNLAQV+SN I+GCI++K+N++ ILI AQCFGIVV LIY FGNSV+AYMCGRVLDAFVN++ +IS++AIIS+LF
Sbjct:    1 MMSIPESSLYTILPKYLVDQLNNVTANTTANTTVNATINITFNVTLNDRINLRINISKLCHIQKPPYFIVSMVFLSKNLAQVVSNLIIGCIIEKINHYFILIAAQCFGIVVALIYAFGNSVVAYMCGRVLDAFVNAASYISILAIISSLF 150
[Close]
BLAST of SMED30027753 vs. Planmine SMEST
Match: SMESG000041557.1 (SMESG000041557.1)

HSP 1 Score: 326.25 bits (835), Expect = 1.623e-124
Identity = 189/308 (61.36%), Postives = 227/308 (73.70%), Query Frame = -3 
Query:  841 FSDINTRGQYFGLGNTLYFFSHSIGAVYGAEIDSRLGKSPVFFFXXXXXXXXXXXXXXLCKNVSNGFQNDDKNEAGIKTIYNKDIIETNNESEGNKKEIQKQVINSDQSPDKDSDFCPMKV--KPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGI 1758
            FS++NTRG++FGLGNTLYF SHSIG VYGAE+D RLGKS VFF LIPLWI T+  T +L +       N  KN    K        ETN   + N+ ++   + +  ++P+K SDF   K   K NK   +   +++K P+V  CL QLFI N SMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGL+ L  K A +  F++PII+Q+I GILLILLP SHDWRL+FIPIIGMYF VG++EQV+FPLTT+LAD  FNSDYG+M+SLTSQS+T+  V GTLSSGPIA+RFGF  L I LGI
Sbjct:  150 FSNVNTRGRFFGLGNTLYFVSHSIGPVYGAEVDVRLGKSAVFFLLIPLWIFTMAATFILYRRTEKR-CNKSKNNCNEKLSIGVTPDETNELDDHNELKVAIIISSISKTPEKYSDFKGEKKFQKENKEIFMKFYKIMKNPYVSSCLCQLFIVNLSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLIVLLLKCAYQLRFFSPIIAQIIVGILLILLPLSHDWRLLFIPIIGMYFSVGVIEQVLFPLTTYLADHHFNSDYGSMASLTSQSLTLSGVVGTLSSGPIAQRFGFRSLCICLGI 456

HSP 2 Score: 140.969 bits (354), Expect = 1.623e-124
Identity = 78/162 (48.15%), Postives = 104/162 (64.20%), Query Frame = -2 
Query: 1757 MQFNCGSVLVILMVSLNSFMITIPESTLYTILPKYFVDKHRSSXXXXXXXXXXXXXXXXNLRINISKL---CPLKKPPYFMVSMVFLAKNLAQVISNSIVGCIVDKVNNFTILITAQCFGIVVTLIYGFGNSVIAYMCGRVLDAFVNSSLFISVMAIISNLF 2233
            M F C   L  +++ LN +M+ I ES+LYTILPKY VD+  ++T N               + N S+    C L+KPPYF+VSM+FL+K+LAQ   NSI+GC VDKVNNF IL+  Q  GI++ LIY   NS+  Y+ GR+LDAFV+ SLFI   +I+S LF
Sbjct:    1 MSFKCDDCLAAIVICLNFYMLGIQESSLYTILPKYLVDEPFNATNNET------------FKPNTSRTIFQCHLQKPPYFIVSMIFLSKSLAQFFFNSIIGCFVDKVNNFIILLIGQGLGIIIALIYALNNSITMYIVGRLLDAFVSCSLFIGGFSIVSKLF 150
[Close]
BLAST of SMED30027753 vs. Planmine SMEST
Match: SMESG000069017.1 (SMESG000069017.1)

HSP 1 Score: 272.322 bits (695), Expect = 6.993e-104
Identity = 152/328 (46.34%), Postives = 220/328 (67.07%), Query Frame = -3 
Query:  793 SQIFFSDINTRGQYFGLGNTLYFFSHSIGAVYGAEIDSRLGKSPVFFFXXXXXXXXXXXXXXLCKNVSNGFQNDDKNEAGIKTIYNKDIIETNNESEGNKKEIQKQVINSDQSPDKDSDFCPMKVKP--NKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLLKNSN 1770
            SQ+F  +   R +Y+G  NTL+F SHS+G VYGA +D + GKS VF  LIP+W +++L T LL + +    +N       ++ + N +  +T  +S  N+    K +I S+ S   ++    ++ K   N   L +I +MLK P VL CL+Q+F+ N  MF+LP TLPSWM F+LC++E+QQG+VW+FGF+GY+ GV LV +   YAP+F    P+  Q+I GI LI++PF+ DWR +F+PIIGMY  VG+VEQ+I PLTT+L+D  +NS+YGTMSSLTSQS+ +C V  +L SGPIAE++GF  L+I+LGI+NL F  +  + +  N
Sbjct:  239 SQMFL-ETRIRSKYYGFVNTLFFVSHSLGPVYGASVDEKFGKSAVFLLLIPIWSISLLFTYLLHRRIKTINKNKKGVPRFLEIMENHNWNDTRVDSPNNE---SKNLILSESSEKNETIKVKIENKQSGNHRTLFTIFKMLKDPKVLACLVQIFVVNLCMFSLPPTLPSWMAFKLCSSELQQGIVWSFGFLGYILGVSLVIIIDAYAPRFRNLAPVAGQIIIGIQLIMIPFTRDWRFLFLPIIGMYISVGVVEQIILPLTTYLSDSLYNSNYGTMSSLTSQSLNLCSVISSLCSGPIAEKYGFTTLAIALGIINLSFSPISLIHRKIN 562

HSP 2 Score: 126.331 bits (316), Expect = 6.993e-104
Identity = 70/154 (45.45%), Postives = 100/154 (64.94%), Query Frame = -2 
Query: 1751 VLVILMVSLNSFMITIPESTLYTILPKYFVDKHRSSXXXXXXXXXXXXXXXXNLRINISKLCPLKKPPYFMVSMVFLAKNLAQVISNSIVGCIVDKVNNFTILITAQCFGIVVTLIYGFGNSVIAYMCGRVLDAFVNSSLFISVMAIISNLFFR 2212
            +LVI++V LN FM+ I ES+LYTILPKY  ++      N + I             N+S  C  K PPY ++SM+FL+KNLA +  N I+G I+D++NNF ILI AQ  G+ V L+Y F NS++ Y+ GR++DAFV S +F++V +IIS +F  
Sbjct:  101 LLVIIVVGLNFFMMVIEESSLYTILPKYLSNRTFGEINNYSRIYAS----------NLSMKCVSKDPPYVIISMIFLSKNLATIFINPIIGMILDRMNNFIILIFAQILGVSVLLVYAFSNSILMYIIGRIIDAFVTSVIFLAVYSIISQMFLE 244
[Close]
BLAST of SMED30027753 vs. Planmine SMEST
Match: SMESG000064367.1 (SMESG000064367.1)

HSP 1 Score: 229.565 bits (584), Expect = 5.069e-79
Identity = 140/314 (44.59%), Postives = 197/314 (62.74%), Query Frame = -3 
Query:  826 FFSDINTRGQYFGLGNTLYFFSHSIGAVYGAEIDSRLGKSPVFFFXXXXXXXXXXXXXXLCKNVSNGFQNDDKNEAGIKTIYNKDIIETNNE--SEGNKKEIQKQVINSDQSPDKDSDFCPMKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFRLCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGILLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGTMSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIF 1761
             F  I  R +Y+ L N +YF SHSIG  YGAE+D R GKS VF  L+PLWIV +L+T+ L     N      ++E  ++    K  +E+ +E  +E N + + K          K   F     K  K+ L  I + ++ PFV++CL QLF+ N SM++LP TLPSWM    C++EI+QG+VW FGF+GY+ GV  V +  K+A +F +Y P++  ++ GIL+I+LPFS DW++VF+PI+GMY  VG V+Q++ PLT +LA     S+   +SSLTSQS+TI  V  TL  G I   FGFH L+I+LGI+NLIF
Sbjct:  128 LFIVIEVRDRYYALVNIIYFISHSIGPFYGAEVDERYGKSTVFLILVPLWIVCLLLTVFLYWRNRN---RSKRSEIKLEGQRQKPNLESLDECINEANVQILDKA---------KMDIFVQNSRKKTKT-LNIIYKFIRDPFVMLCLGQLFVVNLSMYSLPPTLPSWMASEFCSSEIEQGIVWTFGFLGYIVGVVTVIIVDKHAYRFRYYVPVVGHLMIGILMIILPFSRDWKMVFLPIVGMYVGVGFVDQIVIPLTINLAIFRHKSNTNIVSSLTSQSLTISSVISTLLCGHIVMLFGFHFLTITLGIINLIF 428

HSP 2 Score: 85.8853 bits (211), Expect = 5.069e-79
Identity = 48/139 (34.53%), Postives = 81/139 (58.27%), Query Frame = -2 
Query: 1757 ITIPESTLYTILPKYFVDKHRSSXXXXXXXXXXXXXXXXNLRINISKLCPLKKPPYFMVSMVFLAKNLAQVISNSIVGCIVDKVNNFTILITAQCFGIVVTLIYGFGNSVIAYMCGRVLDAFVNSSLFISVMAIISNLF 2173
            + + E++LY ILPKYF ++   S  N   +          +   + + C  +KPPY +VS +FL+KN+A +  N  +G I+ K+N + ILI +   GI V+ IY + ++++ Y+ GRVLD+F+   + I +  +IS LF
Sbjct:    1 MVLQENSLYAILPKYFSNQTSISARNETVL----------INSTVPQNCSFQKPPYVVVSQIFLSKNVAMIFLNIFIGIIIYKLNIYIILILSHFVGIGVSFIYAYTDTLLLYIIGRVLDSFITCGILIGIYFLISKLF 129
The following BLAST results are available for this feature:
BLAST of SMED30027753 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SLC18A11.263e-1524.14solute carrier family 18 member A1 [Source:HGNC Sy... [more]
SLC18A11.263e-1524.14solute carrier family 18 member A1 [Source:HGNC Sy... [more]
SLC18A24.951e-1524.14solute carrier family 18 member A2 [Source:HGNC Sy... [more]
SLC18A33.447e-1124.02solute carrier family 18 member A3 [Source:HGNC Sy... [more]
SLC18A15.803e-1124.42solute carrier family 18 member A1 [Source:HGNC Sy... [more]
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BLAST of SMED30027753 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
unc-171.779e-1224.08Vesicular acetylcholine transporter unc-17 [Sourc... [more]
cat-13.663e-1022.51pep chromosome:WBcel235:X:5705719:5710625:-1 gene:... [more]
cat-14.588e-1022.51pep chromosome:WBcel235:X:5705719:5710585:-1 gene:... [more]
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BLAST of SMED30027753 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
VAChT1.042e-1322.61gene:FBgn0270928 transcript:FBtr0083671[more]
Vmat5.013e-1126.04gene:FBgn0260964 transcript:FBtr0310062[more]
Vmat5.013e-1126.04gene:FBgn0260964 transcript:FBtr0091491[more]
Vmat5.013e-1126.04gene:FBgn0260964 transcript:FBtr0091493[more]
Vmat6.486e-1126.04gene:FBgn0260964 transcript:FBtr0091492[more]
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BLAST of SMED30027753 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
slc18a29.659e-1725.12solute carrier family 18 member 2 [Source:ZFIN;Acc... [more]
SLC18A11.687e-1627.09solute carrier family 18 member A1 [Source:NCBI ge... [more]
SLC18A11.707e-1627.09solute carrier family 18 member A1 [Source:NCBI ge... [more]
SLC18A12.113e-1627.09solute carrier family 18 member A1 [Source:NCBI ge... [more]
SLC18A12.572e-1627.09solute carrier family 18 member A1 [Source:NCBI ge... [more]
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BLAST of SMED30027753 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ddx421.249e-1427.09DEAD-box helicase 42 [Source:Xenbase;Acc:XB-GENE-9... [more]
ddx421.333e-1427.09DEAD-box helicase 42 [Source:Xenbase;Acc:XB-GENE-9... [more]
ddx422.545e-1428.43DEAD-box helicase 42 [Source:Xenbase;Acc:XB-GENE-9... [more]
ddx422.461e-1328.72DEAD-box helicase 42 [Source:Xenbase;Acc:XB-GENE-9... [more]
gtf2e1.23.301e-1324.14general transcription factor IIE, polypeptide 1, a... [more]
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BLAST of SMED30027753 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Slc18a11.872e-1625.82solute carrier family 18 (vesicular monoamine), me... [more]
Slc18a22.254e-1524.14solute carrier family 18 (vesicular monoamine), me... [more]
Slc18a33.085e-1224.58solute carrier family 18 (vesicular monoamine), me... [more]
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BLAST of SMED30027753 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q01818|VMAT1_RAT4.325e-1626.11Chromaffin granule amine transporter OS=Rattus nor... [more]
sp|Q8R090|VMAT1_MOUSE1.310e-1525.82Chromaffin granule amine transporter OS=Mus muscul... [more]
sp|P54219|VMAT1_HUMAN6.063e-1524.14Chromaffin granule amine transporter OS=Homo sapie... [more]
sp|Q8BRU6|VMAT2_MOUSE1.576e-1424.14Synaptic vesicular amine transporter OS=Mus muscul... [more]
sp|Q05940|VMAT2_HUMAN2.378e-1424.14Synaptic vesicular amine transporter OS=Homo sapie... [more]
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BLAST of SMED30027753 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0H3YJ271.766e-12057.78Slc18a-1 OS=Schmidtea mediterranea OX=79327 GN=slc... [more]
A0A0H3YK887.257e-7944.73Slc18a-8 OS=Schmidtea mediterranea OX=79327 GN=slc... [more]
A0A2I6QBP95.247e-1929.63Vesicular acetylcholine transporter (Fragment) OS=... [more]
A0A5N5STS32.347e-1827.01Vesicular acetylcholine transporter OS=Armadillidi... [more]
A0A444T4C93.241e-1826.54Vesicular acetylcholine transporter OS=Armadillidi... [more]
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BLAST of SMED30027753 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
slc18a21.484e-1826.60synaptic vesicular amine transporter [Source:NCBI ... [more]
SLC18A17.306e-1626.60solute carrier family 18 member A1 [Source:NCBI ge... [more]
SLC18A18.424e-1626.60solute carrier family 18 member A1 [Source:NCBI ge... [more]
slc18a3b2.428e-1422.11solute carrier family 18 member 3b [Source:NCBI ge... [more]
slc18a3a5.216e-1224.08solute carrier family 18 member A3 [Source:NCBI ge... [more]
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BLAST of SMED30027753 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
slc18a3a7.125e-1727.14solute carrier family 18 member 3a [Source:NCBI ge... [more]
slc18a21.613e-1424.63solute carrier family 18 member 2 [Source:ZFIN;Acc... [more]
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BLAST of SMED30027753 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027753 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027753 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SLC18A13.167e-1626.79solute carrier family 18 member A1 [Source:NCBI ge... [more]
SLC18A15.817e-1626.79solute carrier family 18 member A1 [Source:NCBI ge... [more]
slc18a3a9.240e-1424.24solute carrier family 18 member 3a [Source:NCBI ge... [more]
slc18a3b5.473e-1321.53probable vesicular acetylcholine transporter-B [So... [more]
slc18a21.595e-726.40solute carrier family 18 member A2 [Source:NCBI ge... [more]
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BLAST of SMED30027753 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000041534.19.806e-42100.00SMESG000041534.1[more]
SMESG000041535.12.898e-18081.37SMESG000041535.1[more]
SMESG000041557.11.623e-12461.36SMESG000041557.1[more]
SMESG000069017.16.993e-10446.34SMESG000069017.1[more]
SMESG000064367.15.069e-7944.59SMESG000064367.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027753 ID=SMED30027753|Name=SMED30027753|organism=Schmidtea mediterranea sexual|type=transcript|length=2267bp
AGAGAACTATAAACAACAATTAAACTTCGACAAAGTCCAACGCAAAAACA
GATACCACAGTGTGGACATCAAAAGAATGAATTCAAAACCAAAAACACCT
ATGGACAGAACACAAAAAAAGAAATACTTTAATTTAAACATTTCATTCAT
GAGTGGCACACTCGACTTTGAGATTCAAAAAGAACTCAATCGCACTTGCC
TGCCAATTTGTATATCCCACAAGTCTTGGACACTAAGGCAAGGACTGGCA
AACAACACAAAAAGAGAAAGCATTCGCACAATGGAAAAAGGCAGACACAA
AAGAAAATTTTCTTCCAAAAAATGTTGTATATTGCATCCCATGCAACGAA
TGTAAAGAAATGTACACTGGAAGTACACGAAGGAAATTACATCAAGGAAT
AAAACAAAGCCTAGACTCTGCCGGAAAATCCTCATTATATAGGCATCGCC
AACATTTCAAAAATATAATCAAAATAACAGTTTATTTTCTCGAATTTTGA
AACAGAGTGGGATTCAATTTTTTTTCAAATTCCAGACGTTCTTTTATTTG
TGCCCAAACAAATTTATTCAGTATTTTGCGTTCAATGAGATAAAATGTTT
GCGCTTATTTCCTTATTACTTGAAAGTGGTTTGTGATAAAATCAAAGTGT
TAGTGTGTTGAGTTCTTATTCTGATCGATAGTACAGTGAACTAGATTTGC
AATTTTAACATACCGGACAGTAATTAAAAATAAAGTACAGATTAAAGATT
TAAATATAATATCTATTTTCAAAGAAACGATACTTACAATTTATTAGAGT
TTTTAAGTAAAAAAGGCAAAGCACAAAAAATTAAATTTAATATTCCAAGA
GAAATACTTAGACAATGAAATCCGAATCTCTCAGCTATAGGACCACTAGA
TAATGTTCCAAATACTCTACAAATGGTCATAGATTGAGACGTCAAAGAAG
ACATGGTTCCATAATCGGAATTAAAGTCTCGGTCTGCCAAATGTGTTGTC
AATGGAAAAATTACTTGTTCAACAATTCCCACACAGAAATACATGCCGAT
AATTGGAATGAATACCAAGCGCCAATCGTGACTGAATGGTAATAGTATCA
GTAAAATCCCAGAGATCATCTGGGAAATTATTGGAGTATAGAAGATAAAC
TTTGGAGCATATTTAAAAGTCAAAGTAACTAGTCCTACACCGCTCACATA
TCCAATAAATCCAAACGCCCAAACTAATCCTTGCTGGATTTCCGTAGCGC
ATAATCGAAACCCCATCCATGAAGGCAGTGTAGCTGGTAACGCAAACATA
CTAAAGTTTGCAATAAATAATTGAATAAGACAGATTAAAACAAAAGGAGT
TTTAAGCATCCGCATTATTGAAATCAGAGGACTTTTATTCGGCTTAACTT
TCATTGGACAGAAATCAGAATCTTTATCAGGACTCTGATCTGAATTTATA
ACTTGTTTTTGGATTTCTTTCTTATTTCCTTCGCTTTCATTATTAGTCTC
AATAATGTCTTTATTATATATCGTTTTAATACCAGCTTCATTTTTATCAT
CATTCTGGAAACCATTTGAAACATTTTTGCACAGTAAAATTGTTACCAAA
ATTGTAACAATCCATAAGGGTATGAGGAAAAAGAAAACTGGGGATTTTCC
TAACCTTGAGTCTATTTCTGCTCCATATACAGCGCCAATAGAATGACTGA
AAAAATATAAAGTATTACCTAAACCAAAATATTGACCTCTCGTGTTGATA
TCTGAAAAAAAGATTTGAGATTATTGCCATGACTGATATAAATAGCGACG
AGTTAACGAATGCATCGAGAACTCTTCCACACATATAAGCTATCACCGAA
TTCCCAAAACCATAAATCAACGTAACAACAATACCAAAACATTGCGCTGT
TATTAAAATGGTAAAATTGTTTACTTTATCAACAATACAACCAACAATTG
AATTCGAAATCACTTGTGCCAGGTTTTTGGCCAGGAATACCATGCTAACC
ATGAAATATGGTGGTTTTTTAAGCGGGCAAAGTTTTGAAATGTTTATCCT
TAAATTGGTTGTGTCATTGACAGTGACATCAACAATGGTATTGTTATTGG
TAGTAGAACTTCGATGTTTATCGACAAAGTATTTTGGAAGAATTGTATAC
AAAGTGCTTTCTGGAATAGTTATCATGAAAGAATTCAAGCTCACCATAAG
AATCACTAAAACACTACCACAATTGAATTGCATTGAATGTTATTCACAGT
GAACTTCATTGTAAGAG
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protein sequence of SMED30027753-orf-1

>SMED30027753-orf-1 ID=SMED30027753-orf-1|Name=SMED30027753-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=118bp
ENYKQQLNFDKVQRKNRYHSVDIKRMNSKPKTPMDRTQKKKYFNLNISFM
SGTLDFEIQKELNRTCLPICISHKSWTLRQGLANNTKRESIRTMEKGRHK
RKFSSKKCCILHPMQRM*
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protein sequence of SMED30027753-orf-2

>SMED30027753-orf-2 ID=SMED30027753-orf-2|Name=SMED30027753-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=173bp
MQFNCGSVLVILMVSLNSFMITIPESTLYTILPKYFVDKHRSSTTNNNTI
VDVTVNDTTNLRINISKLCPLKKPPYFMVSMVFLAKNLAQVISNSIVGCI
VDKVNNFTILITAQCFGIVVTLIYGFGNSVIAYMCGRVLDAFVNSSLFIS
VMAIISNLFFRYQHERSIFWFR*
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protein sequence of SMED30027753-orf-3

>SMED30027753-orf-3 ID=SMED30027753-orf-3|Name=SMED30027753-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=207bp
MKVKPNKSPLISIMRMLKTPFVLICLIQLFIANFSMFALPATLPSWMGFR
LCATEIQQGLVWAFGFIGYVSGVGLVTLTFKYAPKFIFYTPIISQMISGI
LLILLPFSHDWRLVFIPIIGMYFCVGIVEQVIFPLTTHLADRDFNSDYGT
MSSLTSQSMTICRVFGTLSSGPIAERFGFHCLSISLGILNLIFCALPFLL
KNSNKL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR020846MFS_dom
IPR011701MFS
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
Vocabulary: biological process
TermDefinition
GO:0055085transmembrane transport