S-adenosylmethionine synthase

Overview
NameS-adenosylmethionine synthase
Smed IDSMED30027707
Length (bp)1648
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of S-adenosylmethionine synthase (SMED30027707) t-SNE clustered cells

Violin plots show distribution of expression levels for S-adenosylmethionine synthase (SMED30027707) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of S-adenosylmethionine synthase (SMED30027707) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for S-adenosylmethionine synthase (SMED30027707) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30027707

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 14

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
headSMED30027707SMESG000027587.1 SmedASXL_009719SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
pharynxSMED30027707SMESG000027587.1 dd_Smed_v4_1580_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
epidermal cellSMED30027707SMESG000027587.1 dd_Smed_v4_1580_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
headSMED30027707SMESG000027587.1 Contig50933uc_Smed_v2PMID:27612384
Roberts-Galbraith et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
headSMED30027707SMESG000027587.1 Contig50933newmark_estsPMID:27612384
Roberts-Galbraith et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
Category 2 cellSMED30027707SMESG000027587.1 dd_Smed_v6_1580_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30027707SMESG000027587.1 dd_Smed_v6_1580_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30027707SMESG000027587.1 dd_Smed_v6_1580_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neural progenitor cellSMED30027707SMESG000027587.1 dd_Smed_v6_1580_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
secretory systemSMED30027707SMESG000027587.1 dd_Smed_v6_1580_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30027707SMESG000027587.1 dd_Smed_v4_1580_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
dorsal region of the epidermisSMED30027707SMESG000027587.1 dd_Smed_v4_1580_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30027707SMESG000027587.1 SMED_00341_V2_1rGPL14150PMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30027707SMESG000027587.1 SMED_00341_V2_1rGPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of S-adenosylmethionine synthase vs. Ensembl Human
Match: MAT2A (methionine adenosyltransferase 2A [Source:HGNC Symbol;Acc:HGNC:6904])

HSP 1 Score: 590.112 bits (1520), Expect = 0.000e+0
Identity = 290/380 (76.32%), Postives = 333/380 (87.63%), Query Frame = 3
Query:  135 NTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
             TFLFTSESVGEGHPDKICDQISDA+LDAHL+QDP+AKVACET AKTGMIL+ GEI+S+A VDYQKVVRE +K IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVHL+R+ ++IGAGDQGLMFGYATDET ECMPLT+VLAH LN +L+E RRNGTLPWLRPD+KTQVTV+Y  D GA +P+RVHT+VIS QHDE +    ++  L +++IK V+P  YLDE+TIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVK GLCRRVLVQVSYAIGV+HPLSI +  YGTS  S+ ELL IV  NFDLRPG IV++L+L+ PIY++TA YGHFGR+ F WE PKKLK+
Sbjct:   16 GTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGRDSFPWEVPKKLKY 395          
BLAST of S-adenosylmethionine synthase vs. Ensembl Human
Match: MAT1A (methionine adenosyltransferase 1A [Source:HGNC Symbol;Acc:HGNC:6903])

HSP 1 Score: 579.711 bits (1493), Expect = 0.000e+0
Identity = 281/382 (73.56%), Postives = 336/382 (87.96%), Query Frame = 3
Query:  129 SSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            S   F+FTSESVGEGHPDKICDQISDA+LDAHLKQDPNAKVACET  KTGM+L+ GEI+S A+VDYQ+VVR+TIK IGYD   KGFD+KTCNVL+A+E+QSPDIAQ VHL+R+ +++GAGDQGLMFGYATDET ECMPLT++LAH LN R+++ RR+G LPWLRPD+KTQVTV+Y  DNGA IP+R+HT+VIS QH+E I+ + +++ L +++I+ V+P  YLDE+T+YHL PSGRFVIGGPQGDAG+TGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVA PLSI + +YGTS  ++ ELL +V  NFDLRPG IV++L+L+ PIY+KTACYGHFGR EF WE P+KL F
Sbjct:   14 SEGVFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGEITSMAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALEQQSPDIAQCVHLDRNEEDVGAGDQGLMFGYATDETEECMPLTIILAHKLNARMADLRRSGLLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHNEDITLEEMRRALKEQVIRAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSQKTERELLDVVHKNFDLRPGVIVRDLDLKKPIYQKTACYGHFGRSEFPWEVPRKLVF 395          
BLAST of S-adenosylmethionine synthase vs. Ensembl Human
Match: MAT2A (methionine adenosyltransferase 2A [Source:HGNC Symbol;Acc:HGNC:6904])

HSP 1 Score: 449.129 bits (1154), Expect = 3.843e-156
Identity = 226/299 (75.59%), Postives = 259/299 (86.62%), Query Frame = 3
Query:  279 MILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVK 1175
            MIL+ GEI+S+A VDYQKVVRE +K IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVHL+R+ ++IGAGDQGLMFGYATDET ECMPLT+VLAH LN +L+E RRNGTLPWLRPD+KTQVTV+Y  D GA +P+RVHT+VIS QHDE +    ++  L +++IK V+P  YLDE+TIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVK GLCRRVLVQVSYAIGV+HPLSI +  YGTS  S+ ELL IV  NFDLRPG IV+
Sbjct:    1 MILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVR 299          
BLAST of S-adenosylmethionine synthase vs. Ensembl Human
Match: MAT1A (methionine adenosyltransferase 1A [Source:HGNC Symbol;Acc:HGNC:6903])

HSP 1 Score: 235.343 bits (599), Expect = 6.750e-75
Identity = 113/186 (60.75%), Postives = 134/186 (72.04%), Query Frame = 3
Query:  156 ESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQ 713
            ESVGEGHPDKICDQISDA+LDAHLKQDPNAKVAC                                         GFD+KTCNVL+A+E+QSPDIAQ VHL+R+ +++GAGDQGLMFGYATDET ECMPLT++LAH LN R+++ RR+G LPWLRPD+KTQVTV+Y  DNGA IP+R+HT+VIS Q
Sbjct:    1 ESVGEGHPDKICDQISDAVLDAHLKQDPNAKVAC-----------------------------------------GFDFKTCNVLVALEQQSPDIAQCVHLDRNEEDVGAGDQGLMFGYATDETEECMPLTIILAHKLNARMADLRRSGLLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQ 145          
BLAST of S-adenosylmethionine synthase vs. Ensembl Celegans
Match: sams-3 (Probable S-adenosylmethionine synthase 3 [Source:UniProtKB/Swiss-Prot;Acc:P50305])

HSP 1 Score: 545.814 bits (1405), Expect = 0.000e+0
Identity = 271/382 (70.94%), Postives = 327/382 (85.60%), Query Frame = 3
Query:  129 SSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            S + FLFTSESV EGHPDK+CDQISDA+LDAHL QDP+AKVACET  KTGMI++ GEI+SKAVVDYQ +VR  IK+IGYD   KGFDYKTCNVL+A+E+QSP+IA GVH+++D D++GAGDQG+MFGYATDET E MPLT++L+H LN++L E RR+G L W+RPD+KTQVT+EY S+ GAC+PLRVHTVVISTQH   IS   L++ LI+++IK VIP + +D++TIYHLNP G F+IGGP GDAGLTGRKIIVDTYGGWGAHGGGAFSGKD +KVDRSAAYAARWVAKSLVK+GLCRR LVQVSYAIGVA PLS+ V S+GTS+ ++ ELL+IV DNFDLRPG I+K+L+L+ PIY+ TA  GHFG  EF WE+P+ L+ 
Sbjct:    2 SQHKFLFTSESVSEGHPDKMCDQISDAVLDAHLAQDPHAKVACETVTKTGMIMLCGEITSKAVVDYQVLVRNVIKKIGYDDSSKGFDYKTCNVLVALEQQSPEIAAGVHVDKDSDDVGAGDQGIMFGYATDETEEAMPLTLLLSHKLNRKLHELRRSGELEWVRPDSKTQVTIEYASEGGACVPLRVHTVVISTQHSPDISLDDLRKELIEKVIKAVIPANLIDDKTIYHLNPCGSFIIGGPMGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDPTKVDRSAAYAARWVAKSLVKSGLCRRCLVQVSYAIGVAKPLSVMVFSFGTSALNEGELLKIVNDNFDLRPGMIIKDLDLKKPIYEPTAENGHFGHNEFPWEQPRHLQI 383          
BLAST of S-adenosylmethionine synthase vs. Ensembl Celegans
Match: sams-4 (Probable S-adenosylmethionine synthase 4 [Source:UniProtKB/Swiss-Prot;Acc:P50306])

HSP 1 Score: 543.502 bits (1399), Expect = 0.000e+0
Identity = 271/378 (71.69%), Postives = 322/378 (85.19%), Query Frame = 3
Query:  141 FLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            FLFTSESV EGHPDK+CDQISDA+LDAHL QDP+AKVACET  KTGMI++ GEI+SKAVVDYQ +VR  IK+IGYD   KGFDYKTCNVL+A+E+QSP+IA GVH+++D D++GAGDQG+MFGYATDET E MPLT++L+H LN++L E RR+G L W+RPD+KTQVT+EY S+ GAC+PLRVHTVVISTQH   IS   L++ LI+++IK VIP + +D++TIYHLNP G F+IGGP GDAGLTGRKIIVDTYGGWGAHGGGAFSGKD +KVDRSAAYAARWVAKSLVKAGLCRR LVQVSYAIGVA PLS+ V S+GTS+ S+ +LL IV DNFDLRPG I+K L+L+ PIY+ TA  GHFG   F WE+P+ LK 
Sbjct:    6 FLFTSESVSEGHPDKMCDQISDAVLDAHLAQDPHAKVACETVTKTGMIMLCGEITSKAVVDYQVLVRNVIKKIGYDDSSKGFDYKTCNVLVALEQQSPEIAAGVHVDKDSDDVGAGDQGIMFGYATDETEEAMPLTLLLSHKLNRKLHELRRSGELEWVRPDSKTQVTIEYASEGGACVPLRVHTVVISTQHSPDISLDDLRKELIEKVIKAVIPANLIDDKTIYHLNPCGSFIIGGPMGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDPTKVDRSAAYAARWVAKSLVKAGLCRRCLVQVSYAIGVAKPLSVMVFSFGTSALSEEDLLTIVNDNFDLRPGKIIKNLDLKRPIYEPTAENGHFGHNNFPWEQPRNLKI 383          
BLAST of S-adenosylmethionine synthase vs. Ensembl Celegans
Match: sams-4 (Probable S-adenosylmethionine synthase 4 [Source:UniProtKB/Swiss-Prot;Acc:P50306])

HSP 1 Score: 543.502 bits (1399), Expect = 0.000e+0
Identity = 271/378 (71.69%), Postives = 322/378 (85.19%), Query Frame = 3
Query:  141 FLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            FLFTSESV EGHPDK+CDQISDA+LDAHL QDP+AKVACET  KTGMI++ GEI+SKAVVDYQ +VR  IK+IGYD   KGFDYKTCNVL+A+E+QSP+IA GVH+++D D++GAGDQG+MFGYATDET E MPLT++L+H LN++L E RR+G L W+RPD+KTQVT+EY S+ GAC+PLRVHTVVISTQH   IS   L++ LI+++IK VIP + +D++TIYHLNP G F+IGGP GDAGLTGRKIIVDTYGGWGAHGGGAFSGKD +KVDRSAAYAARWVAKSLVKAGLCRR LVQVSYAIGVA PLS+ V S+GTS+ S+ +LL IV DNFDLRPG I+K L+L+ PIY+ TA  GHFG   F WE+P+ LK 
Sbjct:    6 FLFTSESVSEGHPDKMCDQISDAVLDAHLAQDPHAKVACETVTKTGMIMLCGEITSKAVVDYQVLVRNVIKKIGYDDSSKGFDYKTCNVLVALEQQSPEIAAGVHVDKDSDDVGAGDQGIMFGYATDETEEAMPLTLLLSHKLNRKLHELRRSGELEWVRPDSKTQVTIEYASEGGACVPLRVHTVVISTQHSPDISLDDLRKELIEKVIKAVIPANLIDDKTIYHLNPCGSFIIGGPMGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDPTKVDRSAAYAARWVAKSLVKAGLCRRCLVQVSYAIGVAKPLSVMVFSFGTSALSEEDLLTIVNDNFDLRPGKIIKNLDLKRPIYEPTAENGHFGHNNFPWEQPRNLKI 383          
BLAST of S-adenosylmethionine synthase vs. Ensembl Celegans
Match: sams-5 (Probable S-adenosylmethionine synthase 5 [Source:UniProtKB/Swiss-Prot;Acc:Q27522])

HSP 1 Score: 542.732 bits (1397), Expect = 0.000e+0
Identity = 272/384 (70.83%), Postives = 325/384 (84.64%), Query Frame = 3
Query:  129 SSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF*K 1280
            S N FLFTSESV EGHPDK+CDQISDA+LDAHL QDP+AKVACET  KTGMI++ GEI+SKAVVDYQ +VR  IK+IGYD   KGFDYKTCNVL+A+E+QSP+IA GVH+++D  ++GAGDQG+MFGYATDET E MPLT++L+H LN +L E RR+G L W+RPD+KTQVT+EY S+ GAC+PLRVHTVVISTQH   IS + L++ LI+++IK VIP + +D +T+YHLNP G FV+GGP GDAGLTGRKIIVDTYGGWGAHGGGAFSGKD +KVDRSAAYAARWVAKSLVKAGLCRR LVQVSYAIGVA PLS+ V S+GTS+ ++AELL+IV DNFDLRPG I+K L+L+  IY+ TA  GHFG  +F WE+ +KLK  K
Sbjct:    2 SKNKFLFTSESVSEGHPDKMCDQISDAVLDAHLAQDPHAKVACETVTKTGMIMLCGEITSKAVVDYQVLVRNVIKKIGYDDSSKGFDYKTCNVLVALEQQSPEIAAGVHVDKDIADVGAGDQGIMFGYATDETEEAMPLTLLLSHKLNYKLHELRRSGELEWVRPDSKTQVTIEYSSEGGACVPLRVHTVVISTQHSPDISLEDLRKELIEKVIKVVIPGNLIDNDTVYHLNPCGSFVVGGPMGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDPTKVDRSAAYAARWVAKSLVKAGLCRRCLVQVSYAIGVAKPLSVMVFSFGTSALNEAELLKIVNDNFDLRPGMIIKNLDLKRAIYEPTAENGHFGHNDFPWEQARKLKIDK 385          
BLAST of S-adenosylmethionine synthase vs. Ensembl Celegans
Match: sams-5 (Probable S-adenosylmethionine synthase 5 [Source:UniProtKB/Swiss-Prot;Acc:Q27522])

HSP 1 Score: 542.732 bits (1397), Expect = 0.000e+0
Identity = 272/384 (70.83%), Postives = 325/384 (84.64%), Query Frame = 3
Query:  129 SSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF*K 1280
            S N FLFTSESV EGHPDK+CDQISDA+LDAHL QDP+AKVACET  KTGMI++ GEI+SKAVVDYQ +VR  IK+IGYD   KGFDYKTCNVL+A+E+QSP+IA GVH+++D  ++GAGDQG+MFGYATDET E MPLT++L+H LN +L E RR+G L W+RPD+KTQVT+EY S+ GAC+PLRVHTVVISTQH   IS + L++ LI+++IK VIP + +D +T+YHLNP G FV+GGP GDAGLTGRKIIVDTYGGWGAHGGGAFSGKD +KVDRSAAYAARWVAKSLVKAGLCRR LVQVSYAIGVA PLS+ V S+GTS+ ++AELL+IV DNFDLRPG I+K L+L+  IY+ TA  GHFG  +F WE+ +KLK  K
Sbjct:    2 SKNKFLFTSESVSEGHPDKMCDQISDAVLDAHLAQDPHAKVACETVTKTGMIMLCGEITSKAVVDYQVLVRNVIKKIGYDDSSKGFDYKTCNVLVALEQQSPEIAAGVHVDKDIADVGAGDQGIMFGYATDETEEAMPLTLLLSHKLNYKLHELRRSGELEWVRPDSKTQVTIEYSSEGGACVPLRVHTVVISTQHSPDISLEDLRKELIEKVIKVVIPGNLIDNDTVYHLNPCGSFVVGGPMGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDPTKVDRSAAYAARWVAKSLVKAGLCRRCLVQVSYAIGVAKPLSVMVFSFGTSALNEAELLKIVNDNFDLRPGMIIKNLDLKRAIYEPTAENGHFGHNDFPWEQARKLKIDK 385          
BLAST of S-adenosylmethionine synthase vs. Ensembl Fly
Match: Sam-S (gene:FBgn0005278 transcript:FBtr0089430)

HSP 1 Score: 535.413 bits (1378), Expect = 0.000e+0
Identity = 277/393 (70.48%), Postives = 323/393 (82.19%), Query Frame = 3
Query:  120 NGSSSN--------TFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            NGS+ N        TFLFTSESVGEGHPDK+CDQISDAILDAHLKQDPNAKVACET AKTGMIL+ GEI+SKAVVDYQKVVRET++ IGYD   KGFD+KTCNVLLA+++QSP+IA GVH+ R  + IGAGDQG+MFGYATDET ECMPLTVVLAH LN++++E RR+    W RPD+KTQVT EY  + G+ +P RVHT+V+S QH E IS + L+  ++++++K VIP  Y+D  TI H+NP G FVIGGP GDAGLTGRKIIVDTYGGWGAHGGGAFSGKDF+KVDRSAAYAARWVAKSLVKAGLC+R LVQVSYAIG+A PLSI V  YGTS  S  ELL I+  NFDLRPG IVK+LNLR PIY++T+ YGHFGR  F+WE+ K L+ 
Sbjct:   14 NGSNGNSYDMEDGATFLFTSESVGEGHPDKMCDQISDAILDAHLKQDPNAKVACETVAKTGMILLCGEITSKAVVDYQKVVRETVQHIGYDDSSKGFDFKTCNVLLALDQQSPEIAAGVHVNRAEEEIGAGDQGIMFGYATDETEECMPLTVVLAHKLNEKIAELRRSDVFWWARPDSKTQVTCEYLFNQGSAVPKRVHTIVVSMQHSEKISLETLRSEVMEKVVKVVIPAKYIDANTIVHINPCGLFVIGGPMGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDFTKVDRSAAYAARWVAKSLVKAGLCKRCLVQVSYAIGLAEPLSITVFDYGTSHKSQKELLDIIRRNFDLRPGKIVKDLNLRQPIYQRTSTYGHFGRAGFSWEEAKPLEI 406          
BLAST of S-adenosylmethionine synthase vs. Ensembl Fly
Match: Sam-S (gene:FBgn0005278 transcript:FBtr0089433)

HSP 1 Score: 535.413 bits (1378), Expect = 0.000e+0
Identity = 277/393 (70.48%), Postives = 323/393 (82.19%), Query Frame = 3
Query:  120 NGSSSN--------TFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            NGS+ N        TFLFTSESVGEGHPDK+CDQISDAILDAHLKQDPNAKVACET AKTGMIL+ GEI+SKAVVDYQKVVRET++ IGYD   KGFD+KTCNVLLA+++QSP+IA GVH+ R  + IGAGDQG+MFGYATDET ECMPLTVVLAH LN++++E RR+    W RPD+KTQVT EY  + G+ +P RVHT+V+S QH E IS + L+  ++++++K VIP  Y+D  TI H+NP G FVIGGP GDAGLTGRKIIVDTYGGWGAHGGGAFSGKDF+KVDRSAAYAARWVAKSLVKAGLC+R LVQVSYAIG+A PLSI V  YGTS  S  ELL I+  NFDLRPG IVK+LNLR PIY++T+ YGHFGR  F+WE+ K L+ 
Sbjct:   14 NGSNGNSYDMEDGATFLFTSESVGEGHPDKMCDQISDAILDAHLKQDPNAKVACETVAKTGMILLCGEITSKAVVDYQKVVRETVQHIGYDDSSKGFDFKTCNVLLALDQQSPEIAAGVHVNRAEEEIGAGDQGIMFGYATDETEECMPLTVVLAHKLNEKIAELRRSDVFWWARPDSKTQVTCEYLFNQGSAVPKRVHTIVVSMQHSEKISLETLRSEVMEKVVKVVIPAKYIDANTIVHINPCGLFVIGGPMGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDFTKVDRSAAYAARWVAKSLVKAGLCKRCLVQVSYAIGLAEPLSITVFDYGTSHKSQKELLDIIRRNFDLRPGKIVKDLNLRQPIYQRTSTYGHFGRAGFSWEEAKPLEI 406          
BLAST of S-adenosylmethionine synthase vs. Ensembl Fly
Match: Sam-S (gene:FBgn0005278 transcript:FBtr0089432)

HSP 1 Score: 535.413 bits (1378), Expect = 0.000e+0
Identity = 277/393 (70.48%), Postives = 323/393 (82.19%), Query Frame = 3
Query:  120 NGSSSN--------TFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            NGS+ N        TFLFTSESVGEGHPDK+CDQISDAILDAHLKQDPNAKVACET AKTGMIL+ GEI+SKAVVDYQKVVRET++ IGYD   KGFD+KTCNVLLA+++QSP+IA GVH+ R  + IGAGDQG+MFGYATDET ECMPLTVVLAH LN++++E RR+    W RPD+KTQVT EY  + G+ +P RVHT+V+S QH E IS + L+  ++++++K VIP  Y+D  TI H+NP G FVIGGP GDAGLTGRKIIVDTYGGWGAHGGGAFSGKDF+KVDRSAAYAARWVAKSLVKAGLC+R LVQVSYAIG+A PLSI V  YGTS  S  ELL I+  NFDLRPG IVK+LNLR PIY++T+ YGHFGR  F+WE+ K L+ 
Sbjct:   14 NGSNGNSYDMEDGATFLFTSESVGEGHPDKMCDQISDAILDAHLKQDPNAKVACETVAKTGMILLCGEITSKAVVDYQKVVRETVQHIGYDDSSKGFDFKTCNVLLALDQQSPEIAAGVHVNRAEEEIGAGDQGIMFGYATDETEECMPLTVVLAHKLNEKIAELRRSDVFWWARPDSKTQVTCEYLFNQGSAVPKRVHTIVVSMQHSEKISLETLRSEVMEKVVKVVIPAKYIDANTIVHINPCGLFVIGGPMGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDFTKVDRSAAYAARWVAKSLVKAGLCKRCLVQVSYAIGLAEPLSITVFDYGTSHKSQKELLDIIRRNFDLRPGKIVKDLNLRQPIYQRTSTYGHFGRAGFSWEEAKPLEI 406          
BLAST of S-adenosylmethionine synthase vs. Ensembl Fly
Match: Sam-S (gene:FBgn0005278 transcript:FBtr0089429)

HSP 1 Score: 535.413 bits (1378), Expect = 0.000e+0
Identity = 277/393 (70.48%), Postives = 323/393 (82.19%), Query Frame = 3
Query:  120 NGSSSN--------TFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            NGS+ N        TFLFTSESVGEGHPDK+CDQISDAILDAHLKQDPNAKVACET AKTGMIL+ GEI+SKAVVDYQKVVRET++ IGYD   KGFD+KTCNVLLA+++QSP+IA GVH+ R  + IGAGDQG+MFGYATDET ECMPLTVVLAH LN++++E RR+    W RPD+KTQVT EY  + G+ +P RVHT+V+S QH E IS + L+  ++++++K VIP  Y+D  TI H+NP G FVIGGP GDAGLTGRKIIVDTYGGWGAHGGGAFSGKDF+KVDRSAAYAARWVAKSLVKAGLC+R LVQVSYAIG+A PLSI V  YGTS  S  ELL I+  NFDLRPG IVK+LNLR PIY++T+ YGHFGR  F+WE+ K L+ 
Sbjct:   14 NGSNGNSYDMEDGATFLFTSESVGEGHPDKMCDQISDAILDAHLKQDPNAKVACETVAKTGMILLCGEITSKAVVDYQKVVRETVQHIGYDDSSKGFDFKTCNVLLALDQQSPEIAAGVHVNRAEEEIGAGDQGIMFGYATDETEECMPLTVVLAHKLNEKIAELRRSDVFWWARPDSKTQVTCEYLFNQGSAVPKRVHTIVVSMQHSEKISLETLRSEVMEKVVKVVIPAKYIDANTIVHINPCGLFVIGGPMGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDFTKVDRSAAYAARWVAKSLVKAGLCKRCLVQVSYAIGLAEPLSITVFDYGTSHKSQKELLDIIRRNFDLRPGKIVKDLNLRQPIYQRTSTYGHFGRAGFSWEEAKPLEI 406          
BLAST of S-adenosylmethionine synthase vs. Ensembl Fly
Match: Sam-S (gene:FBgn0005278 transcript:FBtr0089434)

HSP 1 Score: 535.413 bits (1378), Expect = 0.000e+0
Identity = 277/393 (70.48%), Postives = 323/393 (82.19%), Query Frame = 3
Query:  120 NGSSSN--------TFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            NGS+ N        TFLFTSESVGEGHPDK+CDQISDAILDAHLKQDPNAKVACET AKTGMIL+ GEI+SKAVVDYQKVVRET++ IGYD   KGFD+KTCNVLLA+++QSP+IA GVH+ R  + IGAGDQG+MFGYATDET ECMPLTVVLAH LN++++E RR+    W RPD+KTQVT EY  + G+ +P RVHT+V+S QH E IS + L+  ++++++K VIP  Y+D  TI H+NP G FVIGGP GDAGLTGRKIIVDTYGGWGAHGGGAFSGKDF+KVDRSAAYAARWVAKSLVKAGLC+R LVQVSYAIG+A PLSI V  YGTS  S  ELL I+  NFDLRPG IVK+LNLR PIY++T+ YGHFGR  F+WE+ K L+ 
Sbjct:   14 NGSNGNSYDMEDGATFLFTSESVGEGHPDKMCDQISDAILDAHLKQDPNAKVACETVAKTGMILLCGEITSKAVVDYQKVVRETVQHIGYDDSSKGFDFKTCNVLLALDQQSPEIAAGVHVNRAEEEIGAGDQGIMFGYATDETEECMPLTVVLAHKLNEKIAELRRSDVFWWARPDSKTQVTCEYLFNQGSAVPKRVHTIVVSMQHSEKISLETLRSEVMEKVVKVVIPAKYIDANTIVHINPCGLFVIGGPMGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDFTKVDRSAAYAARWVAKSLVKAGLCKRCLVQVSYAIGLAEPLSITVFDYGTSHKSQKELLDIIRRNFDLRPGKIVKDLNLRQPIYQRTSTYGHFGRAGFSWEEAKPLEI 406          
BLAST of S-adenosylmethionine synthase vs. Ensembl Zebrafish
Match: mat2aa (methionine adenosyltransferase II, alpha a [Source:ZFIN;Acc:ZDB-GENE-030131-2049])

HSP 1 Score: 589.341 bits (1518), Expect = 0.000e+0
Identity = 288/380 (75.79%), Postives = 336/380 (88.42%), Query Frame = 3
Query:  135 NTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            + FLFTSESVGEGHPDKICDQISDA+LDAHLKQDP+AKVACET AKTGMIL+ GEI+S AVVDYQKVVR+TIK IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVHL+R+ +++GAGDQGLMFGYATDET ECMPLT+VLAH LN +++E RRNGTLPWLRPD+KTQVTV+Y+ D GA +P+RVHT+V+S QHD+ +    ++  L ++++K V+P  YLD++TIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVKAGLC+RVLVQVSYAIGVAHPLS+ +  YGTS  S+ ELL IV  NFDLRPG IV+EL+L+ PIY++TA YGHFGRE F WE PKKLK+
Sbjct:   16 DCFLFTSESVGEGHPDKICDQISDAVLDAHLKQDPDAKVACETVAKTGMILLAGEITSHAVVDYQKVVRDTIKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDVGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKMAELRRNGTLPWLRPDSKTQVTVQYRQDRGAMLPVRVHTIVVSVQHDDVVCLDEMRDALKEKVVKAVVPNVYLDDDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCKRVLVQVSYAIGVAHPLSVSIFHYGTSQRSEKELLEIVKKNFDLRPGVIVRELDLKKPIYQRTAAYGHFGRESFPWEVPKKLKY 395          
BLAST of S-adenosylmethionine synthase vs. Ensembl Zebrafish
Match: mat1a (methionine adenosyltransferase I, alpha [Source:ZFIN;Acc:ZDB-GENE-030131-6127])

HSP 1 Score: 579.711 bits (1493), Expect = 0.000e+0
Identity = 285/378 (75.40%), Postives = 334/378 (88.36%), Query Frame = 3
Query:  138 TFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLK 1271
            +F+FTSESVGEGHPDKICDQISDA+LDAHLKQDP+AKVACET  KTGM+L+ GEI+S+A VDYQK+VR+TIK IGYD+ EKGFDYKTCNVL+A+E+QSPDIAQGVH++R  ++IGAGDQGLMFGYATDET ECMPLT+VLAH LN +++E RR+GT+PWLRPD+KTQVTV YK +NGA IPLRVHTVVIS QHD+ IS +  Q+ L +++IK V+P  YLD++T+YHL PSGRFVIGGPQGDAG+TGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVK+ LCRRVLVQVSYAIGVAHPLSI + +YG+S  ++ ELL IV  NFDLRPG IV++LNL+ P Y+ TACYGHFGR EF WE  K LK
Sbjct:   12 SFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPDAKVACETVCKTGMVLLCGEITSRANVDYQKIVRDTIKHIGYDNSEKGFDYKTCNVLVALEQQSPDIAQGVHVDRHEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNSKMAELRRDGTIPWLRPDSKTQVTVHYKQENGAVIPLRVHTVVISVQHDDNISLEEQQRILKEKVIKAVVPARYLDDKTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKSKLCRRVLVQVSYAIGVAHPLSISLFTYGSSEKTEKELLHIVNKNFDLRPGVIVRDLNLKRPFYQNTACYGHFGRSEFPWEMAKTLK 389          
BLAST of S-adenosylmethionine synthase vs. Ensembl Zebrafish
Match: mat1a (methionine adenosyltransferase I, alpha [Source:ZFIN;Acc:ZDB-GENE-030131-6127])

HSP 1 Score: 579.711 bits (1493), Expect = 0.000e+0
Identity = 285/378 (75.40%), Postives = 334/378 (88.36%), Query Frame = 3
Query:  138 TFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLK 1271
            +F+FTSESVGEGHPDKICDQISDA+LDAHLKQDP+AKVACET  KTGM+L+ GEI+S+A VDYQK+VR+TIK IGYD+ EKGFDYKTCNVL+A+E+QSPDIAQGVH++R  ++IGAGDQGLMFGYATDET ECMPLT+VLAH LN +++E RR+GT+PWLRPD+KTQVTV YK +NGA IPLRVHTVVIS QHD+ IS +  Q+ L +++IK V+P  YLD++T+YHL PSGRFVIGGPQGDAG+TGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVK+ LCRRVLVQVSYAIGVAHPLSI + +YG+S  ++ ELL IV  NFDLRPG IV++LNL+ P Y+ TACYGHFGR EF WE  K LK
Sbjct:   12 SFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPDAKVACETVCKTGMVLLCGEITSRANVDYQKIVRDTIKHIGYDNSEKGFDYKTCNVLVALEQQSPDIAQGVHVDRHEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNSKMAELRRDGTIPWLRPDSKTQVTVHYKQENGAVIPLRVHTVVISVQHDDNISLEEQQRILKEKVIKAVVPARYLDDKTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKSKLCRRVLVQVSYAIGVAHPLSISLFTYGSSEKTEKELLHIVNKNFDLRPGVIVRDLNLKRPFYQNTACYGHFGRSEFPWEMAKTLK 389          
BLAST of S-adenosylmethionine synthase vs. Ensembl Zebrafish
Match: mat2ab (methionine adenosyltransferase II, alpha b [Source:ZFIN;Acc:ZDB-GENE-050327-6])

HSP 1 Score: 539.65 bits (1389), Expect = 0.000e+0
Identity = 269/347 (77.52%), Postives = 307/347 (88.47%), Query Frame = 3
Query:  135 NTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVK 1175
            + FLFTSESVGEGHPDKICDQISDA+LDAHLKQDP+AKVACET AKTGMIL+ GE++S+AVVDYQKVVR+TIK IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVHLERD  ++GAGDQGLMFGYATDET ECMPLT+VLAH LN +++E RRNGTLPWLRPD+KTQVTV+Y+ ++GA +P+RVHT+VIS QHDE I    ++  L  ++I  V+P  YLD++TIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVKA LCRRVLVQVSYAIGVAHPLSI +  YGTS  S+ ELL+IV  NFDLRPG IV+
Sbjct:   17 DCFLFTSESVGEGHPDKICDQISDAVLDAHLKQDPDAKVACETVAKTGMILLAGEVTSRAVVDYQKVVRDTIKHIGYDDSTKGFDYKTCNVLVALEQQSPDIAQGVHLERDEQDVGAGDQGLMFGYATDETEECMPLTIVLAHKLNSKMAELRRNGTLPWLRPDSKTQVTVQYRQEHGAMLPIRVHTIVISVQHDEDICLDEMRDALKDKVINTVVPSMYLDDDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAKLCRRVLVQVSYAIGVAHPLSISIFHYGTSQKSEQELLKIVKKNFDLRPGVIVR 363          
BLAST of S-adenosylmethionine synthase vs. Ensembl Zebrafish
Match: mat2al (methionine adenosyltransferase II, alpha-like [Source:ZFIN;Acc:ZDB-GENE-060421-5255])

HSP 1 Score: 528.094 bits (1359), Expect = 0.000e+0
Identity = 262/382 (68.59%), Postives = 318/382 (83.25%), Query Frame = 3
Query:  129 SSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            S  TFLFTSESVGEGH DK+CDQISDA+LDA+L QDP+++VACE  +KTGMIL+ GE++SKAVVD Q+VVR+T+K IGYD   KGFDYKTCNVL++++ Q  +I+  V   RD ++IGAGDQGLMFGYATDET ECMPLT++LAH LN ++ E  + G  PW+ PD+KTQVTVEY+ + GA  P+RVHTVVIS QH   I+ + ++  L+++++K VIP  YLD++TIYHL PSG+F++GGPQGDAGLTGRKIIVDTYGGWG HGGGAFSGKD+SKVDRS AYAARWVAKSLVKA LCRRVLVQ+SYAIG++HPLS+ V  YGTS+  + +LL IV  NFDLRPG IVKEL+L+ PIY+KTACYGHFGRE F WEKPKKL F
Sbjct:    9 SGKTFLFTSESVGEGHSDKMCDQISDAVLDAYLTQDPDSRVACECVSKTGMILLCGEVTSKAVVDLQRVVRDTVKTIGYDDSSKGFDYKTCNVLVSLQPQVSEISDCVFEGRDSEDIGAGDQGLMFGYATDETEECMPLTILLAHKLNYKMKELSKTGVCPWILPDSKTQVTVEYRDNMGAMEPVRVHTVVISVQHSPDITLEDIRHNLMEKVVKAVIPAKYLDDKTIYHLLPSGKFLLGGPQGDAGLTGRKIIVDTYGGWGGHGGGAFSGKDYSKVDRSGAYAARWVAKSLVKAKLCRRVLVQISYAIGISHPLSVSVFHYGTSTRDEDDLLEIVKKNFDLRPGVIVKELDLKKPIYQKTACYGHFGREGFPWEKPKKLIF 390          
BLAST of S-adenosylmethionine synthase vs. Ensembl Xenopus
Match: use1 (unconventional SNARE in the ER 1 homolog [Source:Xenbase;Acc:XB-GENE-972033])

HSP 1 Score: 585.874 bits (1509), Expect = 0.000e+0
Identity = 291/396 (73.48%), Postives = 338/396 (85.35%), Query Frame = 3
Query:  117 MNGSSSN----------TFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            MNG   N          +FLFTSESVGEGHPDKICDQISDA+LDAHLKQDP+AKVACET AKTGMIL+ GEI+S+A VDYQK+VR+TIK IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVHL+R+ +++GAGDQGLMFGYATDET E MPLT+VLAH LN R++E RRNGTLPWLRPD+KTQVTV+Y  D GA IP+RVHT+V+S QHDETI    ++  L ++I+K V+P  YLD++TIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVK+GLCRRVLVQV+YAIGV+HPLSI +  YGTS  S+ ELL IV  NFDLRPG IV++L L+ PIY++TA YGHFGR+ F WE PKKL++
Sbjct:    1 MNGQMLNGFHDDLIDEGSFLFTSESVGEGHPDKICDQISDAVLDAHLKQDPDAKVACETVAKTGMILLAGEITSRASVDYQKIVRDTIKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDVGAGDQGLMFGYATDETEEGMPLTIVLAHKLNARMAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVIPIRVHTIVVSVQHDETICLDEMRDALKEKIVKAVVPAKYLDDDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKSGLCRRVLVQVAYAIGVSHPLSISIFHYGTSQKSERELLAIVKKNFDLRPGVIVRDLELKKPIYQRTAAYGHFGRDSFPWEVPKKLEY 396          
BLAST of S-adenosylmethionine synthase vs. Ensembl Xenopus
Match: use1 (unconventional SNARE in the ER 1 homolog [Source:Xenbase;Acc:XB-GENE-972033])

HSP 1 Score: 585.104 bits (1507), Expect = 0.000e+0
Identity = 287/380 (75.53%), Postives = 334/380 (87.89%), Query Frame = 3
Query:  135 NTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
             +FLFTSESVGEGHPDKICDQISDA+LDAHLKQDP+AKVACET AKTGMIL+ GEI+S+A VDYQK+VR+TIK IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVHL+R+ +++GAGDQGLMFGYATDET E MPLT+VLAH LN R++E RRNGTLPWLRPD+KTQVTV+Y  D GA IP+RVHT+V+S QHDETI    ++  L ++I+K V+P  YLD++TIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVK+GLCRRVLVQV+YAIGV+HPLSI +  YGTS  S+ ELL IV  NFDLRPG IV++L L+ PIY++TA YGHFGR+ F WE PKKL++
Sbjct:   14 GSFLFTSESVGEGHPDKICDQISDAVLDAHLKQDPDAKVACETVAKTGMILLAGEITSRASVDYQKIVRDTIKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDVGAGDQGLMFGYATDETEEGMPLTIVLAHKLNARMAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVIPIRVHTIVVSVQHDETICLDEMRDALKEKIVKAVVPAKYLDDDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKSGLCRRVLVQVAYAIGVSHPLSISIFHYGTSQKSERELLAIVKKNFDLRPGVIVRDLELKKPIYQRTAAYGHFGRDSFPWEVPKKLEY 393          
BLAST of S-adenosylmethionine synthase vs. Ensembl Xenopus
Match: mat1a (methionine adenosyltransferase I, alpha [Source:NCBI gene;Acc:100127858])

HSP 1 Score: 579.711 bits (1493), Expect = 0.000e+0
Identity = 287/378 (75.93%), Postives = 333/378 (88.10%), Query Frame = 3
Query:  141 FLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            F+FTSESVGEGHPDKICDQISDA+LDAHL QDP+AKVACET  KTGM+L+ GEI+S+AVVDYQK+VR+TIK IGYD  EKGFDYKTCNVL+A+E+QSPDIAQGVHL+R  ++IGAGDQGLMFGYATDET ECMPLT+VLAH LN +L+E RRNG LPWLRPD+KTQVTV+Y   NGA IP+RVHT+VIS QHDETIS   +Q+ L + +IK V+P  YLDE+T+YHL PSGRFVIGGPQGDAG+TGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLV A LC RVLVQVSYAIGVA+PLS+ + +YGTS  ++ ELL IV  NFDLRPG IV++L+L+ P+Y+KTACYGHFGRE+F WE PK+L F
Sbjct:   19 FMFTSESVGEGHPDKICDQISDAVLDAHLSQDPDAKVACETVCKTGMVLLCGEITSRAVVDYQKIVRDTIKYIGYDDSEKGFDYKTCNVLVALEQQSPDIAQGVHLDRTEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNSKLAELRRNGVLPWLRPDSKTQVTVKYIQKNGAVIPVRVHTIVISVQHDETISLSDMQEALKEHVIKAVVPAKYLDEKTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVHAKLCHRVLVQVSYAIGVAYPLSVSLFTYGTSEKTEKELLDIVNANFDLRPGVIVRDLDLKKPLYQKTACYGHFGREDFPWEVPKELFF 396          
BLAST of S-adenosylmethionine synthase vs. Ensembl Xenopus
Match: use1 (unconventional SNARE in the ER 1 homolog [Source:Xenbase;Acc:XB-GENE-972033])

HSP 1 Score: 559.681 bits (1441), Expect = 0.000e+0
Identity = 281/390 (72.05%), Postives = 329/390 (84.36%), Query Frame = 3
Query:  111 IKMNG--SSSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            I  NG   S+N FLF          +KICDQISDA+LDAHLKQDP+AKVACET AKTGMIL+ GEI+S+A VDYQK+VR+TIK IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVHL+R+ +++GAGDQGLMFGYATDET E MPLT+VLAH LN R++E RRNGTLPWLRPD+KTQVTV+Y  D GA IP+RVHT+V+S QHDETI    ++  L ++I+K V+P  YLD++TIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVK+GLCRRVLVQV+YAIGV+HPLSI +  YGTS  S+ ELL IV  NFDLRPG IV++L L+ PIY++TA YGHFGR+ F WE PKKL++
Sbjct:    1 IAANGLNPSANHFLFK---------NKICDQISDAVLDAHLKQDPDAKVACETVAKTGMILLAGEITSRASVDYQKIVRDTIKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDVGAGDQGLMFGYATDETEEGMPLTIVLAHKLNARMAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVIPIRVHTIVVSVQHDETICLDEMRDALKEKIVKAVVPAKYLDDDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKSGLCRRVLVQVAYAIGVSHPLSISIFHYGTSQKSERELLAIVKKNFDLRPGVIVRDLELKKPIYQRTAAYGHFGRDSFPWEVPKKLEY 381          
BLAST of S-adenosylmethionine synthase vs. Ensembl Xenopus
Match: use1 (unconventional SNARE in the ER 1 homolog [Source:Xenbase;Acc:XB-GENE-972033])

HSP 1 Score: 554.673 bits (1428), Expect = 0.000e+0
Identity = 274/365 (75.07%), Postives = 320/365 (87.67%), Query Frame = 3
Query:  180 DKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            DKICDQISDA+LDAHLKQDP+AKVACET AKTGMIL+ GEI+S+A VDYQK+VR+TIK IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVHL+R+ +++GAGDQGLMFGYATDET E MPLT+VLAH LN R++E RRNGTLPWLRPD+KTQVTV+Y  D GA IP+RVHT+V+S QHDETI    ++  L ++I+K V+P  YLD++TIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVK+GLCRRVLVQV+YAIGV+HPLSI +  YGTS  S+ ELL IV  NFDLRPG IV++L L+ PIY++TA YGHFGR+ F WE PKKL++
Sbjct:   19 DKICDQISDAVLDAHLKQDPDAKVACETVAKTGMILLAGEITSRASVDYQKIVRDTIKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDVGAGDQGLMFGYATDETEEGMPLTIVLAHKLNARMAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVIPIRVHTIVVSVQHDETICLDEMRDALKEKIVKAVVPAKYLDDDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKSGLCRRVLVQVAYAIGVSHPLSISIFHYGTSQKSERELLAIVKKNFDLRPGVIVRDLELKKPIYQRTAAYGHFGRDSFPWEVPKKLEY 383          
BLAST of S-adenosylmethionine synthase vs. Ensembl Mouse
Match: Mat2a (methionine adenosyltransferase II, alpha [Source:MGI Symbol;Acc:MGI:2443731])

HSP 1 Score: 589.726 bits (1519), Expect = 0.000e+0
Identity = 290/380 (76.32%), Postives = 333/380 (87.63%), Query Frame = 3
Query:  135 NTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
             TFLFTSESVGEGHPDKICDQISDA+LDAHL+QDP+AKVACET AKTGMIL+ GEI+S+A +DYQKVVRE IK IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVHL+R+ ++IGAGDQGLMFGYATDET ECMPLT+VLAH LN +L+E RRNGTLPWLRPD+KTQVTV+Y  D GA +P+RVHT+VIS QHDE +    ++  L +++IK V+P  YLDE+TIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVK GLCRRVLVQVSYAIGV+HPLSI +  YGTS  S+ ELL IV  NFDLRPG IV++L+L+ PIY++TA YGHFGR+ F WE PKKLK+
Sbjct:   16 GTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAIDYQKVVREAIKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGRDSFPWEVPKKLKY 395          
BLAST of S-adenosylmethionine synthase vs. Ensembl Mouse
Match: Mat2a (methionine adenosyltransferase II, alpha [Source:MGI Symbol;Acc:MGI:2443731])

HSP 1 Score: 589.726 bits (1519), Expect = 0.000e+0
Identity = 290/380 (76.32%), Postives = 333/380 (87.63%), Query Frame = 3
Query:  135 NTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
             TFLFTSESVGEGHPDKICDQISDA+LDAHL+QDP+AKVACET AKTGMIL+ GEI+S+A +DYQKVVRE IK IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVHL+R+ ++IGAGDQGLMFGYATDET ECMPLT+VLAH LN +L+E RRNGTLPWLRPD+KTQVTV+Y  D GA +P+RVHT+VIS QHDE +    ++  L +++IK V+P  YLDE+TIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVK GLCRRVLVQVSYAIGV+HPLSI +  YGTS  S+ ELL IV  NFDLRPG IV++L+L+ PIY++TA YGHFGR+ F WE PKKLK+
Sbjct:   16 GTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAIDYQKVVREAIKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGRDSFPWEVPKKLKY 395          
BLAST of S-adenosylmethionine synthase vs. Ensembl Mouse
Match: Mat1a (methionine adenosyltransferase I, alpha [Source:MGI Symbol;Acc:MGI:88017])

HSP 1 Score: 585.104 bits (1507), Expect = 0.000e+0
Identity = 285/383 (74.41%), Postives = 337/383 (87.99%), Query Frame = 3
Query:  126 SSSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            S    F+FTSESVGEGHPDKICDQISDA+LDAHLKQDPNAKVACET  KTGM+L+ GEI+S A+VDYQ+VVR+TIK IGYD   KGFD+KTCNVL+A+E+QSPDIAQ VHL+R+ +++GAGDQGLMFGYATDET ECMPLT+VLAH LN R+++ RR+G LPWLRPD+KTQVTV+Y  DNGA IP+R+HT+VIS QH+E I+ + +Q+ L +++IK V+P  YLDE+T+YHL PSGRFVIGGPQGDAG+TGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVA PLSI + +YGTS+ ++ ELL +V  NFDLRPG IV++L+L+ PIY+KTACYGHFGR EF WE PKKL F
Sbjct:   14 SEEGAFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGEITSVAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALEQQSPDIAQCVHLDRNEEDVGAGDQGLMFGYATDETEECMPLTIVLAHKLNTRIADLRRSGVLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHNEDITLEAMQEALKEQVIKAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSNKTERELLEVVNKNFDLRPGVIVRDLDLKKPIYQKTACYGHFGRSEFPWEVPKKLVF 396          
BLAST of S-adenosylmethionine synthase vs. Ensembl Mouse
Match: Mat1a (methionine adenosyltransferase I, alpha [Source:MGI Symbol;Acc:MGI:88017])

HSP 1 Score: 585.104 bits (1507), Expect = 0.000e+0
Identity = 285/383 (74.41%), Postives = 337/383 (87.99%), Query Frame = 3
Query:  126 SSSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            S    F+FTSESVGEGHPDKICDQISDA+LDAHLKQDPNAKVACET  KTGM+L+ GEI+S A+VDYQ+VVR+TIK IGYD   KGFD+KTCNVL+A+E+QSPDIAQ VHL+R+ +++GAGDQGLMFGYATDET ECMPLT+VLAH LN R+++ RR+G LPWLRPD+KTQVTV+Y  DNGA IP+R+HT+VIS QH+E I+ + +Q+ L +++IK V+P  YLDE+T+YHL PSGRFVIGGPQGDAG+TGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVA PLSI + +YGTS+ ++ ELL +V  NFDLRPG IV++L+L+ PIY+KTACYGHFGR EF WE PKKL F
Sbjct:   14 SEEGAFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPNAKVACETVCKTGMVLLCGEITSVAMVDYQRVVRDTIKHIGYDDSAKGFDFKTCNVLVALEQQSPDIAQCVHLDRNEEDVGAGDQGLMFGYATDETEECMPLTIVLAHKLNTRIADLRRSGVLPWLRPDSKTQVTVQYMQDNGAVIPVRIHTIVISVQHNEDITLEAMQEALKEQVIKAVVPAKYLDEDTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAEPLSISIFTYGTSNKTERELLEVVNKNFDLRPGVIVRDLDLKKPIYQKTACYGHFGRSEFPWEVPKKLVF 396          
BLAST of S-adenosylmethionine synthase vs. Ensembl Mouse
Match: Mat2a (methionine adenosyltransferase II, alpha [Source:MGI Symbol;Acc:MGI:2443731])

HSP 1 Score: 540.806 bits (1392), Expect = 0.000e+0
Identity = 270/350 (77.14%), Postives = 307/350 (87.71%), Query Frame = 3
Query:  135 NTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELN 1184
             TFLFTSESVGEGHPDKICDQISDA+LDAHL+QDP+AKVACET AKTGMIL+ GEI+S+A +DYQKVVRE IK IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVHL+R+ ++IGAGDQGLMFGYATDET ECMPLT+VLAH LN +L+E RRNGTLPWLRPD+KTQVTV+Y  D GA +P+RVHT+VIS QHDE +    ++  L +++IK V+P  YLDE+TIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVK GLCRRVLVQVSYAIGV+HPLSI +  YGTS  S+ ELL IV  NFDLRPG IV+ L+
Sbjct:   16 GTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAIDYQKVVREAIKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRVLH 365          
BLAST of S-adenosylmethionine synthase vs. UniProt/SwissProt
Match: sp|P18298|METK2_RAT (S-adenosylmethionine synthase isoform type-2 OS=Rattus norvegicus OX=10116 GN=Mat2a PE=1 SV=1)

HSP 1 Score: 590.497 bits (1521), Expect = 0.000e+0
Identity = 290/380 (76.32%), Postives = 334/380 (87.89%), Query Frame = 3
Query:  135 NTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
             TFLFTSESVGEGHPDKICDQI+DA+LDAHL+QDP+AKVACET AKTGMIL+ GEI+S+A +DYQKVVRE IK IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVHL+R+ ++IGAGDQGLMFGYATDET ECMPLT+VLAH LN +L+E RRNGTLPWLRPD+KTQVTV+Y  D GA IP+RVHT+VIS QHDE +    ++  L +++IK V+P  YLDE+TIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVK GLCRRVLVQVSYAIGV+HPLSI +  YGTS  S+ ELL IV +NFDLRPG IV++L+L+ PIY++TA YGHFGR+ F WE PKKLK+
Sbjct:   16 GTFLFTSESVGEGHPDKICDQINDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAIDYQKVVREAIKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVIPIRVHTIVISVQHDEEVCLDEMRDALKEKLIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKNNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGRDSFPWEVPKKLKY 395          
BLAST of S-adenosylmethionine synthase vs. UniProt/SwissProt
Match: sp|Q5R5H1|METK2_PONAB (S-adenosylmethionine synthase isoform type-2 OS=Pongo abelii OX=9601 GN=MAT2A PE=2 SV=1)

HSP 1 Score: 590.112 bits (1520), Expect = 0.000e+0
Identity = 290/380 (76.32%), Postives = 333/380 (87.63%), Query Frame = 3
Query:  135 NTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
             TFLFTSESVGEGHPDKICDQISDA+LDAHL+QDP+AKVACET AKTGMIL+ GEI+S+A VDYQKVVRE +K IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVHL+R+ ++IGAGDQGLMFGYATDET ECMPLT+VLAH LN +L+E RRNGTLPWLRPD+KTQVTV+Y  D GA +P+RVHT+VIS QHDE +    ++  L +++IK V+P  YLDE+TIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVK GLCRRVLVQVSYAIGV+HPLSI +  YGTS  S+ ELL IV  NFDLRPG IV++L+L+ PIY++TA YGHFGR+ F WE PKKLK+
Sbjct:   16 GTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGRDSFPWEVPKKLKY 395          
BLAST of S-adenosylmethionine synthase vs. UniProt/SwissProt
Match: sp|Q4R924|METK2_MACFA (S-adenosylmethionine synthase isoform type-2 OS=Macaca fascicularis OX=9541 GN=MAT2A PE=2 SV=1)

HSP 1 Score: 590.112 bits (1520), Expect = 0.000e+0
Identity = 290/380 (76.32%), Postives = 333/380 (87.63%), Query Frame = 3
Query:  135 NTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
             TFLFTSESVGEGHPDKICDQISDA+LDAHL+QDP+AKVACET AKTGMIL+ GEI+S+A VDYQKVVRE +K IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVHL+R+ ++IGAGDQGLMFGYATDET ECMPLT+VLAH LN +L+E RRNGTLPWLRPD+KTQVTV+Y  D GA +P+RVHT+VIS QHDE +    ++  L +++IK V+P  YLDE+TIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVK GLCRRVLVQVSYAIGV+HPLSI +  YGTS  S+ ELL IV  NFDLRPG IV++L+L+ PIY++TA YGHFGR+ F WE PKKLK+
Sbjct:   16 GTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGRDSFPWEVPKKLKY 395          
BLAST of S-adenosylmethionine synthase vs. UniProt/SwissProt
Match: sp|P31153|METK2_HUMAN (S-adenosylmethionine synthase isoform type-2 OS=Homo sapiens OX=9606 GN=MAT2A PE=1 SV=1)

HSP 1 Score: 590.112 bits (1520), Expect = 0.000e+0
Identity = 290/380 (76.32%), Postives = 333/380 (87.63%), Query Frame = 3
Query:  135 NTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
             TFLFTSESVGEGHPDKICDQISDA+LDAHL+QDP+AKVACET AKTGMIL+ GEI+S+A VDYQKVVRE +K IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVHL+R+ ++IGAGDQGLMFGYATDET ECMPLT+VLAH LN +L+E RRNGTLPWLRPD+KTQVTV+Y  D GA +P+RVHT+VIS QHDE +    ++  L +++IK V+P  YLDE+TIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVK GLCRRVLVQVSYAIGV+HPLSI +  YGTS  S+ ELL IV  NFDLRPG IV++L+L+ PIY++TA YGHFGR+ F WE PKKLK+
Sbjct:   16 GTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAVDYQKVVREAVKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGRDSFPWEVPKKLKY 395          
BLAST of S-adenosylmethionine synthase vs. UniProt/SwissProt
Match: sp|Q3THS6|METK2_MOUSE (S-adenosylmethionine synthase isoform type-2 OS=Mus musculus OX=10090 GN=Mat2a PE=1 SV=2)

HSP 1 Score: 589.726 bits (1519), Expect = 0.000e+0
Identity = 290/380 (76.32%), Postives = 333/380 (87.63%), Query Frame = 3
Query:  135 NTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
             TFLFTSESVGEGHPDKICDQISDA+LDAHL+QDP+AKVACET AKTGMIL+ GEI+S+A +DYQKVVRE IK IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVHL+R+ ++IGAGDQGLMFGYATDET ECMPLT+VLAH LN +L+E RRNGTLPWLRPD+KTQVTV+Y  D GA +P+RVHT+VIS QHDE +    ++  L +++IK V+P  YLDE+TIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVK GLCRRVLVQVSYAIGV+HPLSI +  YGTS  S+ ELL IV  NFDLRPG IV++L+L+ PIY++TA YGHFGR+ F WE PKKLK+
Sbjct:   16 GTFLFTSESVGEGHPDKICDQISDAVLDAHLQQDPDAKVACETVAKTGMILLAGEITSRAAIDYQKVVREAIKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKLAELRRNGTLPWLRPDSKTQVTVQYMQDRGAVLPIRVHTIVISVQHDEEVCLDEMRDALKEKVIKAVVPAKYLDEDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKGGLCRRVLVQVSYAIGVSHPLSISIFHYGTSQKSERELLEIVKKNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGRDSFPWEVPKKLKY 395          
BLAST of S-adenosylmethionine synthase vs. TrEMBL
Match: A0A1I8I605 (S-adenosylmethionine synthase OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008771g3 PE=3 SV=1)

HSP 1 Score: 622.854 bits (1605), Expect = 0.000e+0
Identity = 302/385 (78.44%), Postives = 345/385 (89.61%), Query Frame = 3
Query:  120 NGSSSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            NG    TFLFTSESVGEGHPDKICDQ+SDA+LDAHLKQDPNAKVACE F KTGMILVGGEI+SKAVVDYQ+V+R TIK+IGYD   KGFDYKTCNVL+A+EEQSPDIAQGVH  R  D+IGAGDQGLMFGYATDET ECMPLT+VLAH LN +L++CRR+GTLPWLRPDTKTQVTVEYK +NGAC+PLR+HT+V+STQHDE+++ + L++ +++ ++K V P   LDE TIYHLNPSGRFVIGGPQ DAGLTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAAYAARWVAKSLVK+GLCRRVLVQVSYAIGV+ PL++Y++SYGT   SD ELLRIV +NFDLRPG IV++L LR PIY+KT+CYGHFGR+EFTWE+ K LKF
Sbjct:    3 NGEGLKTFLFTSESVGEGHPDKICDQVSDAVLDAHLKQDPNAKVACECFTKTGMILVGGEITSKAVVDYQRVIRGTIKDIGYDDSNKGFDYKTCNVLVALEEQSPDIAQGVHEARSEDDIGAGDQGLMFGYATDETVECMPLTIVLAHKLNAQLAQCRRDGTLPWLRPDTKTQVTVEYKDENGACVPLRIHTIVVSTQHDESVTQEQLRKDVMEHVVKAVCPSDMLDENTIYHLNPSGRFVIGGPQSDAGLTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAAYAARWVAKSLVKSGLCRRVLVQVSYAIGVSKPLAVYLNSYGTGKRSDEELLRIVEENFDLRPGCIVRDLELRKPIYQKTSCYGHFGRDEFTWEQAKPLKF 387          
BLAST of S-adenosylmethionine synthase vs. TrEMBL
Match: A0A267DP28 (S-adenosylmethionine synthase OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008771g2 PE=3 SV=1)

HSP 1 Score: 622.468 bits (1604), Expect = 0.000e+0
Identity = 302/385 (78.44%), Postives = 344/385 (89.35%), Query Frame = 3
Query:  120 NGSSSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            NG    TFLFTSESVGEGHPDKICDQ+SDA+LDAHLKQDPNAKVACE F KTGMILVGGEI+SKAVVDYQ+V+R TIK+IGYD   KGFDYKTCNVL+A+EEQSPDIAQGVH  R  D+IGAGDQGLMFGYATDET ECMPLT+VLAH LN +L++CRR+GTLPWLRPDTKTQVTVEYK +NGAC+PLR+HT+V+STQHDE+++ + L++ ++  ++K V P   LDE TIYHLNPSGRFVIGGPQ DAGLTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAAYAARWVAKSLVK+GLCRRVLVQVSYAIGV+ PL++Y++SYGT   SD ELLRIV +NFDLRPG IV++L LR PIY+KT+CYGHFGR+EFTWE+ K LKF
Sbjct:    3 NGEGLKTFLFTSESVGEGHPDKICDQVSDAVLDAHLKQDPNAKVACECFTKTGMILVGGEITSKAVVDYQRVIRGTIKDIGYDDSNKGFDYKTCNVLVALEEQSPDIAQGVHEARSEDDIGAGDQGLMFGYATDETVECMPLTIVLAHKLNAQLAQCRRDGTLPWLRPDTKTQVTVEYKDENGACVPLRIHTIVVSTQHDESVTQEQLRKDVMDHVVKAVCPSDMLDENTIYHLNPSGRFVIGGPQSDAGLTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAAYAARWVAKSLVKSGLCRRVLVQVSYAIGVSKPLAVYLNSYGTGKRSDEELLRIVEENFDLRPGCIVRDLELRKPIYQKTSCYGHFGRDEFTWEQAKPLKF 387          
BLAST of S-adenosylmethionine synthase vs. TrEMBL
Match: A0A1I8HKQ4 (S-adenosylmethionine synthase OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig008771g1 PE=3 SV=1)

HSP 1 Score: 620.157 bits (1598), Expect = 0.000e+0
Identity = 301/385 (78.18%), Postives = 344/385 (89.35%), Query Frame = 3
Query:  120 NGSSSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            NG    TFLFTSESVGEGHPDKICDQ+SDA+LDAHLKQDPNAKVACE F KTGMILVGGEI+SKAVVDYQ+V+R TIK+IGYD   KGFDYKTCNVL+A+EEQSPDIAQGVH  R  D+IGAGDQGLMFGYATDET ECMPLT+VLAH LN +L++CRR+GTLPWLRPDTKTQVTVEYK +NGAC+PLR+HT+V+STQHDE+++ + L++ +++ ++K V P   LDE TIYHLNPSGRFVIGGPQ DAGLTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAAYAARWVAKSLVK+GLCRRVLVQVSYAIGV+ PL++Y++SYGT   SD ELL IV +NFDLRPG IV++L LR PIY+KT+CYGHFGR+EFTWE+ K LKF
Sbjct:    3 NGEGLKTFLFTSESVGEGHPDKICDQVSDAVLDAHLKQDPNAKVACECFTKTGMILVGGEITSKAVVDYQRVIRGTIKDIGYDDSNKGFDYKTCNVLVALEEQSPDIAQGVHEARSEDDIGAGDQGLMFGYATDETVECMPLTIVLAHKLNAQLAQCRRDGTLPWLRPDTKTQVTVEYKDENGACVPLRIHTIVVSTQHDESVTQEQLRKDVMEHVVKAVCPSDMLDENTIYHLNPSGRFVIGGPQSDAGLTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAAYAARWVAKSLVKSGLCRRVLVQVSYAIGVSKPLAVYLNSYGTGKRSDEELLHIVEENFDLRPGCIVRDLELRKPIYQKTSCYGHFGRDEFTWEQAKPLKF 387          
BLAST of S-adenosylmethionine synthase vs. TrEMBL
Match: A0A267H2L8 (S-adenosylmethionine synthase OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig014959g3 PE=3 SV=1)

HSP 1 Score: 613.609 bits (1581), Expect = 0.000e+0
Identity = 297/384 (77.34%), Postives = 340/384 (88.54%), Query Frame = 3
Query:  123 GSSSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            G    TFLFTSESVGEGHPDKICDQ+SDA+LDAHLKQD NAKVACE F KTGMI+VGGEISS A+VDYQ+V+RETI++IGYD   KGFDYKTCNVL+A+EEQSPDIAQGVH+ R  D+IGAGDQGLMFGYATDET ECMPLT+VLAH LN RL++CRR+GTLPWLRPDTKTQVTVEYK +NGAC+PLR+HT+V+STQHD+ I+ + L+  +++ ++K V P+  LDE T+YHLNPSGRFVIGGPQ DAGLTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAAYAARWVAKSLVKA LCRRVLVQ+SYAIGVA PL++Y++SYGT + SD ELLRIV  NFDLRPG IVK+L LR PIY+KT+CYGHFG  EF+WE  K+LKF
Sbjct:    6 GEGFKTFLFTSESVGEGHPDKICDQVSDAVLDAHLKQDANAKVACECFTKTGMIMVGGEISSTAIVDYQRVIRETIRQIGYDDSSKGFDYKTCNVLVALEEQSPDIAQGVHVARADDDIGAGDQGLMFGYATDETEECMPLTIVLAHGLNARLAQCRRDGTLPWLRPDTKTQVTVEYKDENGACVPLRIHTIVVSTQHDDNITQEKLRHDVMEHVVKAVCPKEMLDERTVYHLNPSGRFVIGGPQSDAGLTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAAYAARWVAKSLVKAKLCRRVLVQISYAIGVAKPLAVYLNSYGTGAKSDEELLRIVETNFDLRPGRIVKDLELRKPIYQKTSCYGHFGNPEFSWEMAKELKF 389          
BLAST of S-adenosylmethionine synthase vs. TrEMBL
Match: A0A267EMZ1 (S-adenosylmethionine synthase OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig014959g2 PE=3 SV=1)

HSP 1 Score: 612.068 bits (1577), Expect = 0.000e+0
Identity = 296/384 (77.08%), Postives = 340/384 (88.54%), Query Frame = 3
Query:  123 GSSSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            G    TFLFTSESVGEGHPDKICDQ+SDA+LDAHLKQD NAKVACE F KTGMI+VGGEISS A+VDYQ+V+RETI++IGYD   KGFDYKTCNVL+A+EEQSPDIAQGVH+ R  D+IGAGDQGLMFGYATDET ECMPLT+VLAH LN RL++CRR+GTLPWLRPDTKTQVTVEYK +NGAC+PLR+HT+V+STQHD+ I+ + L+  +++ ++K V P+  LDE T+YHLNPSGRFVIGGPQ DAGLTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAAYAARWVAKSLVKA LCRRVLVQ+SYAIGVA PL++Y++SYGT + SD ELLRIV  NFDLRPG IVK+L LR PIY+KT+CYGHFG  EF+WE  K+L+F
Sbjct:    6 GEGFKTFLFTSESVGEGHPDKICDQVSDAVLDAHLKQDANAKVACECFTKTGMIMVGGEISSTAIVDYQRVIRETIRQIGYDDSSKGFDYKTCNVLVALEEQSPDIAQGVHVARADDDIGAGDQGLMFGYATDETEECMPLTIVLAHGLNARLAQCRRDGTLPWLRPDTKTQVTVEYKDENGACVPLRIHTIVVSTQHDDNITQEKLRHDVMEHVVKAVCPKEMLDERTVYHLNPSGRFVIGGPQSDAGLTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAAYAARWVAKSLVKAKLCRRVLVQISYAIGVAKPLAVYLNSYGTGAKSDEELLRIVETNFDLRPGRIVKDLELRKPIYQKTSCYGHFGNPEFSWEMAKELQF 389          
BLAST of S-adenosylmethionine synthase vs. Ensembl Cavefish
Match: mat2ab (S-adenosylmethionine synthase [Source:NCBI gene;Acc:103044681])

HSP 1 Score: 581.252 bits (1497), Expect = 0.000e+0
Identity = 288/392 (73.47%), Postives = 339/392 (86.48%), Query Frame = 3
Query:  114 KMNGSSS-----NTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            +MNG  +     + FLFTSESVGEGHPDKICDQISDA+LDAHLKQDP+AKVACET AKTGMIL+ GE++S+A VDYQKVVR+TI+ IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVHL+R+ +++GAGDQGLMFGYATDET ECMPLT+VLAH LN +++E RRNG LPWLRPD+KTQVTV+Y+ D GA +P+RVHT+V+S QHDE I    ++  L ++++K V+P  YLD++TIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVKA LCRRVLVQVSYAIGVAHPLSI +  YGTS  S+ ELL IV  NFDLRPG IV++L LR PIY++TA YGHFGR+ F+WE PKKL++
Sbjct:    9 EMNGFHNALIDEDCFLFTSESVGEGHPDKICDQISDAVLDAHLKQDPDAKVACETVAKTGMILLAGEVTSRATVDYQKVVRDTIRHIGYDDSTKGFDYKTCNVLVALEQQSPDIAQGVHLDRNQEDVGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKMAELRRNGVLPWLRPDSKTQVTVQYRQDRGAMLPVRVHTIVVSVQHDEDICLDEMRDALKEKVVKAVVPCVYLDDDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAELCRRVLVQVSYAIGVAHPLSISIFHYGTSQKSERELLDIVRKNFDLRPGVIVRDLQLRKPIYQRTAAYGHFGRDSFSWEIPKKLQY 400          
BLAST of S-adenosylmethionine synthase vs. Ensembl Cavefish
Match: mat1a (S-adenosylmethionine synthase [Source:NCBI gene;Acc:103041217])

HSP 1 Score: 579.711 bits (1493), Expect = 0.000e+0
Identity = 285/379 (75.20%), Postives = 336/379 (88.65%), Query Frame = 3
Query:  138 TFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            +F+FTSESVGEGHPDKICDQISDA+LDAHL+QDP+AKVACET  KTGM+L+ GEI+S+A VDYQKVVR+TIK IGYD+ +KGFDYKTCNVL+A+E+QSPDIAQGVH++R  ++IGAGDQGLMFGYATDET ECMPLT+VLAH LN +++E RR+GT+PWLRPD+KTQVTV YK +NGA IPLRVHTVVIS QHD+ IS +  Q+ L +++IK V+P  YLD++T+YHL PSGRFVIGGPQGDAG+TGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLV A LC+RVLVQVSYAIGVAHPLSI + +YG+S  ++ ELL+IV  NFDLRPG IV++L L+ PIY+ TACYGHFGR EF WE PK LKF
Sbjct:   12 SFMFTSESVGEGHPDKICDQISDAVLDAHLRQDPDAKVACETVCKTGMVLLCGEITSRANVDYQKVVRDTIKHIGYDNSDKGFDYKTCNVLVALEQQSPDIAQGVHIDRHEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNSKMAELRRDGTIPWLRPDSKTQVTVHYKQENGAVIPLRVHTVVISVQHDDLISLEEQQRILKEKVIKAVVPAKYLDDKTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVMAKLCKRVLVQVSYAIGVAHPLSISLFTYGSSEKTEKELLQIVNKNFDLRPGVIVRDLKLKRPIYQDTACYGHFGRSEFPWEVPKTLKF 390          
BLAST of S-adenosylmethionine synthase vs. Ensembl Cavefish
Match: mat1a (S-adenosylmethionine synthase [Source:NCBI gene;Acc:103041217])

HSP 1 Score: 570.852 bits (1470), Expect = 0.000e+0
Identity = 285/390 (73.08%), Postives = 336/390 (86.15%), Query Frame = 3
Query:  138 TFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIV-----------KELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            +F+FTSESVGEGHPDKICDQISDA+LDAHL+QDP+AKVACET  KTGM+L+ GEI+S+A VDYQKVVR+TIK IGYD+ +KGFDYKTCNVL+A+E+QSPDIAQGVH++R  ++IGAGDQGLMFGYATDET ECMPLT+VLAH LN +++E RR+GT+PWLRPD+KTQVTV YK +NGA IPLRVHTVVIS QHD+ IS +  Q+ L +++IK V+P  YLD++T+YHL PSGRFVIGGPQGDAG+TGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLV A LC+RVLVQVSYAIGVAHPLSI + +YG+S  ++ ELL+IV  NFDLRPG IV           ++L L+ PIY+ TACYGHFGR EF WE PK LKF
Sbjct:   12 SFMFTSESVGEGHPDKICDQISDAVLDAHLRQDPDAKVACETVCKTGMVLLCGEITSRANVDYQKVVRDTIKHIGYDNSDKGFDYKTCNVLVALEQQSPDIAQGVHIDRHEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNSKMAELRRDGTIPWLRPDSKTQVTVHYKQENGAVIPLRVHTVVISVQHDDLISLEEQQRILKEKVIKAVVPAKYLDDKTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVMAKLCKRVLVQVSYAIGVAHPLSISLFTYGSSEKTEKELLQIVNKNFDLRPGVIVRVFQTWAVNNYRDLKLKRPIYQDTACYGHFGRSEFPWEVPKTLKF 401          
BLAST of S-adenosylmethionine synthase vs. Ensembl Cavefish
Match: mat2aa (S-adenosylmethionine synthase [Source:NCBI gene;Acc:103039813])

HSP 1 Score: 569.311 bits (1466), Expect = 0.000e+0
Identity = 288/380 (75.79%), Postives = 335/380 (88.16%), Query Frame = 3
Query:  135 NTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            + FLFTSESVGEGHPDKICDQISDA+LDAHLKQDP+AKVACET AKTGMIL+ GE++S+A VDYQKVVR+ IK IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVHL+R+ ++IGAGDQGLMFGYATDET ECMPLT+VLAH LN +++E RRNGTLPWLRPD+KTQVTV+Y+ D GA +P+RVHTVV+S QHDE +    ++  L ++++K V+P  YLD++TIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVKAGLC+RVLVQVSYAIGVAHPLSI +  YGTS  S+ ELL IV  NFDLRPG IV++L+L+ PIY++TA YGHFGRE F WE PKKLK+
Sbjct:   16 DCFLFTSESVGEGHPDKICDQISDAVLDAHLKQDPDAKVACETVAKTGMILLAGEVTSRACVDYQKVVRDAIKHIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRNEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKMAELRRNGTLPWLRPDSKTQVTVQYRQDRGAMLPVRVHTVVVSVQHDEVVCLDEMRDALKEKVVKAVVPSVYLDDDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCKRVLVQVSYAIGVAHPLSISIFHYGTSQRSEKELLDIVKKNFDLRPGVIVRDLDLKKPIYQRTAAYGHFGRESFPWEVPKKLKY 395          
BLAST of S-adenosylmethionine synthase vs. Ensembl Cavefish
Match: mat2al (S-adenosylmethionine synthase [Source:NCBI gene;Acc:103022635])

HSP 1 Score: 503.056 bits (1294), Expect = 3.952e-176
Identity = 258/383 (67.36%), Postives = 307/383 (80.16%), Query Frame = 3
Query:  129 SSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQ-GDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            S  TFLFTSESVG+GH DK+CDQISDA+LDA+L QDP+AKVACE  AKTGMIL+ GE++SKAVVD +KVVR+T+K+IGYD   KGFDYKTCNVL+A++ Q  +I   V   RD ++IGAGDQG MFGYATDET ECMPLT++LAH LN ++ E  +NG   W+ PD+K+QVTVEY+ + G   P+RVHTVVIS QH   I+   +++ L+++++K VIP  YLD+ TIYHL PSG+F+IGGPQ        RKIIVDTYGGWG HGGGAFSGKD+SKVDRS AYAARWVAKSLVKA LCRRVLVQ+SYAIG++HPLSI V  YGTS+  + ELL IV  NFDLRPG IVKEL L+ PIY+KTA YGHFGREEF WEKPKKL F
Sbjct:    9 SGKTFLFTSESVGQGHSDKMCDQISDAVLDAYLSQDPDAKVACECVAKTGMILLCGEVTSKAVVDLEKVVRDTVKDIGYDDSSKGFDYKTCNVLVALQPQCEEITDCVFEGRDEEDIGAGDQGFMFGYATDETDECMPLTILLAHKLNHKMKELSKNGECAWILPDSKSQVTVEYRDNMGVMEPIRVHTVVISVQHTPDITLSEIRKNLMEKVVKTVIPAKYLDDRTIYHLLPSGKFLIGGPQVKKCRPYWRKIIVDTYGGWGGHGGGAFSGKDYSKVDRSGAYAARWVAKSLVKAKLCRRVLVQISYAIGISHPLSISVFHYGTSTRDEDELLEIVQKNFDLRPGVIVKELGLKRPIYQKTASYGHFGREEFPWEKPKKLVF 391          
BLAST of S-adenosylmethionine synthase vs. Ensembl Yeast
Match: SAM2 (S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; comparative analysis suggests that a mitochondrially targeted form may result from translation starting at a non-canonical codon upstream of the annotated start codon [Source:SGD;Acc:S000002910])

HSP 1 Score: 550.436 bits (1417), Expect = 0.000e+0
Identity = 268/383 (69.97%), Postives = 323/383 (84.33%), Query Frame = 3
Query:  126 SSSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            S S TFLFTSESVGEGHPDKICDQ+SDAILDA L+QDP +KVACET AKTGMI+V GEI++KA +DYQ++VR+TIK+IGYD   KGFDYKTCNVL+AIE+QSPDIAQG+H E+  +++GAGDQG+MFGYATDET E +PLT++LAH LN  +++ RR+G+LPWLRPDTKTQVTVEY+ DNG  +P R+ TVVIS QH + IS   L+  L K+I++KVIP+  LDE T Y + PSGRFVIGGPQGDAGLTGRKIIVD YGG  + GGGAFSGKD+SKVDRSAAYAARWVAKSLV AGLC+RV VQ SYAIG+A PLS++VD+YGT++ SD E++ I+  NFDLRPG +VKEL+L  PIY  TA YGHF  +E++WEKPKKL+F
Sbjct:    2 SKSKTFLFTSESVGEGHPDKICDQVSDAILDACLEQDPFSKVACETAAKTGMIMVFGEITTKARLDYQQIVRDTIKKIGYDDSAKGFDYKTCNVLVAIEQQSPDIAQGLHYEKSLEDLGAGDQGIMFGYATDETPEGLPLTILLAHKLNMAMADARRDGSLPWLRPDTKTQVTVEYEDDNGRWVPKRIDTVVISAQHADEISTADLRTQLQKDIVEKVIPKDMLDENTKYFIQPSGRFVIGGPQGDAGLTGRKIIVDAYGGASSVGGGAFSGKDYSKVDRSAAYAARWVAKSLVAAGLCKRVQVQFSYAIGIAEPLSLHVDTYGTATKSDDEIIEIIKKNFDLRPGVLVKELDLARPIYLPTASYGHFTNQEYSWEKPKKLEF 384          
BLAST of S-adenosylmethionine synthase vs. Ensembl Yeast
Match: SAM1 (S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication [Source:SGD;Acc:S000004170])

HSP 1 Score: 543.888 bits (1400), Expect = 0.000e+0
Identity = 267/381 (70.08%), Postives = 319/381 (83.73%), Query Frame = 3
Query:  132 SNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            + TFLFTSESVGEGHPDKICDQ+SDAILDA L +DP++KVACET AKTGMI+V GEI++KA +DYQK+VR+TIK+IGYD   KGFDYKTCNVL+AIE+QSPDIAQGVH E+D ++IGAGDQG+MFGYATDET E +PLT++LAH LN  +++ RR+G+L WLRPDTKTQVTVEYK D+G  +P R+ TVV+S QH + I+ + L+  L  EII+KVIP   LDE T Y + PSGRFVIGGPQGDAGLTGRKIIVD YGG  + GGGAFSGKD+SKVDRSAAYAARWVAKSLV AGLC+RV VQ SYAIG+A PLS++VD+YGT++ SD E++ I+  NFDLRPG +VKEL+L  PIY  TA YGHF  +E+ WEKPK LKF
Sbjct:    2 AGTFLFTSESVGEGHPDKICDQVSDAILDACLAEDPHSKVACETAAKTGMIMVFGEITTKAQLDYQKIVRDTIKKIGYDDSAKGFDYKTCNVLVAIEQQSPDIAQGVHEEKDLEDIGAGDQGIMFGYATDETPEGLPLTILLAHKLNMAMADARRDGSLAWLRPDTKTQVTVEYKDDHGRWVPQRIDTVVVSAQHADEITTEDLRAQLKSEIIEKVIPRDMLDENTKYFIQPSGRFVIGGPQGDAGLTGRKIIVDAYGGASSVGGGAFSGKDYSKVDRSAAYAARWVAKSLVAAGLCKRVQVQFSYAIGIAEPLSLHVDTYGTATKSDEEIIDIISKNFDLRPGVLVKELDLARPIYLPTASYGHFTNQEYPWEKPKTLKF 382          
BLAST of S-adenosylmethionine synthase vs. Ensembl Nematostella
Match: EDO37850 (S-adenosylmethionine synthase [Source:UniProtKB/TrEMBL;Acc:A7SEP5])

HSP 1 Score: 568.155 bits (1463), Expect = 0.000e+0
Identity = 282/383 (73.63%), Postives = 336/383 (87.73%), Query Frame = 3
Query:  126 SSSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            + +NTFLFTSESVGEGHPDK+CDQISDAILDAHLKQDPNAKVACET AKTGMIL+ GEI+S AVVDYQ VVR+ IK+IGYD  EKGFDYKTCNVL+A+E+QS DIA GVH+ R+ +++GAGDQGLMFGYATDET E MPLTVVLAH +N++L+E RR+GTLPW RPD+KTQVTVEYK ++G  IPLRVHT+VIS QHD TI+ ++L++ L+++I+K V+P  +LDE+TI+HL PSG FVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARW+AKSLV  GLC+RVLVQ+SYAIG+A PLS+ V SYGTS  S+ ELL+IV DNFDLRPG IVKEL+L+ P+Y+K+  YGHF +  FTWE PK L++
Sbjct:   26 TQNNTFLFTSESVGEGHPDKMCDQISDAILDAHLKQDPNAKVACETVAKTGMILLCGEITSNAVVDYQSVVRQCIKDIGYDDSEKGFDYKTCNVLVALEQQSVDIAHGVHVGREEEDVGAGDQGLMFGYATDETEELMPLTVVLAHKMNQKLAEYRRDGTLPWARPDSKTQVTVEYKFEHGRAIPLRVHTIVISVQHDPTITMENLRKELMEKIVKTVVPSKFLDEDTIFHLQPSGLFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWIAKSLVSEGLCKRVLVQISYAIGIAQPLSVTVFSYGTSKKSEDELLKIVRDNFDLRPGKIVKELDLKKPVYQKSCVYGHF-KPGFTWETPKPLQY 407          
BLAST of S-adenosylmethionine synthase vs. Ensembl Nematostella
Match: EDO37825 (S-adenosylmethionine synthase [Source:UniProtKB/TrEMBL;Acc:A7SEP6])

HSP 1 Score: 547.74 bits (1410), Expect = 0.000e+0
Identity = 279/385 (72.47%), Postives = 323/385 (83.90%), Query Frame = 3
Query:  132 SNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF*KIP 1286
            +NTFLFTSESVGEGHPDK+CDQISDAILDAHLKQDPNAKVACE+ AKTGMI+V GEI+S A VDYQKVVR+TIK+IGYD   KGFDYKTC VL AIE+QSPDIAQGVH+ R  +++GAGDQGLMFGYATDET E MPLTVVLAH LNKRL++CRRNG+LPW+RPD+KTQVTVEYK   G  +PLRVHT+VIS QHD  I    +++ L+  ++K+V+PE Y+D +T+YH+ PSG FVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD SKVDRSAAYAARW+AKSLV A LC RVLVQ+SYAIGVA PLSI V +YGT    + ELL ++  NFDLRPG IV++L+L+ PIY+ T  YGHF +  F WE PK LK+ K P
Sbjct:    8 NNTFLFTSESVGEGHPDKMCDQISDAILDAHLKQDPNAKVACESVAKTGMIVVCGEITSLANVDYQKVVRDTIKQIGYDDSSKGFDYKTCTVLQAIEQQSPDIAQGVHIGRSDEDLGAGDQGLMFGYATDETDELMPLTVVLAHGLNKRLADCRRNGSLPWVRPDSKTQVTVEYKFQGGKAVPLRVHTIVISVQHDPNIELDVMRKELMDYVVKEVVPEKYIDNDTVYHMQPSGSFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDPSKVDRSAAYAARWIAKSLVAAKLCHRVLVQLSYAIGVAEPLSISVFTYGTGYRPEEELLEVIKHNFDLRPGVIVRDLDLKKPIYQSTCLYGHF-KPGFPWEVPKPLKYIKKP 391          
BLAST of S-adenosylmethionine synthase vs. Ensembl Nematostella
Match: EDO45117 (S-adenosylmethionine synthase [Source:UniProtKB/TrEMBL;Acc:A7RTZ9])

HSP 1 Score: 506.523 bits (1303), Expect = 2.212e-177
Identity = 260/383 (67.89%), Postives = 308/383 (80.42%), Query Frame = 3
Query:  126 SSSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            S  + FLFTSESV EGH DK+CDQISDA+LDAHL+QDP AKVACET  KTG++L+ GEI+S A VDYQ VVR TI++IGY+    GFDYKTC+VLLAI+EQ  +IAQ VHL R  D IGAGDQGLMFGYATDET E MPLT VLAH L  RL+ECR+   LPWL PD KTQVTV+Y+ + GACIP RVHTV+ISTQH   +  + ++  L + II  VIPE YLD++TI+HL PSG+F+ GGP GDAGLTGRKIIVDTYGGWGAHGGGAFSGKD +KVDRS AYA RW+AKSLV AGLCRRVLVQV+YAIG+A PLSI V SYG+ +++D +LL ++ DNFDLRPG I+++LNL  PIYK T+ +GHF +  F WE+PKKLK 
Sbjct:   47 SDCDNFLFTSESVNEGHSDKMCDQISDAVLDAHLEQDPYAKVACETATKTGLVLLFGEITSNARVDYQAVVRNTIRDIGYNDSSTGFDYKTCSVLLAIQEQVAEIAQTVHLNRRDDEIGAGDQGLMFGYATDETEELMPLTTVLAHKLCARLAECRKGKILPWLLPDGKTQVTVDYRLERGACIPERVHTVLISTQHTPDVGIEEIRHHLKETIINHVIPEKYLDDKTIFHLLPSGQFIDGGPLGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDVTKVDRSGAYACRWIAKSLVAAGLCRRVLVQVAYAIGIAEPLSISVFSYGSGNYTDKQLLGVIKDNFDLRPGVIIRDLNLLRPIYKATSTFGHF-KPGFPWEEPKKLKL 428          
BLAST of S-adenosylmethionine synthase vs. Ensembl Nematostella
Match: EDO26870 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TAU6])

HSP 1 Score: 224.557 bits (571), Expect = 1.776e-70
Identity = 128/212 (60.38%), Postives = 155/212 (73.11%), Query Frame = 3
Query:  417 IEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYA 1052
            I +QS DI QGV   +  D  GAGDQGLMFGYAT+ET   MP  V  AH L +R SE RRNGTLPWLRPD K+QVT+ ++ D+      +V  VV+STQHD +IS   L++ +++ IIK V+P  +L E T+YH+NP+G FVIGGP GDAGLTGRKIIVDTYGG   HGGGAFSGKD SKVDRSAAY  R+VAK++V A L  +  +QVSYA
Sbjct:    1 IGKQSVDINQGVDRAKPEDQ-GAGDQGLMFGYATNETDSLMPAPVYYAHRLVERQSELRRNGTLPWLRPDAKSQVTINWEGDS-----PKVDAVVLSTQHDPSISLDDLREAVLENIIKPVLPAEWLHEGTLYHINPTGNFVIGGPVGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYIGRYVAKNIVAAELADKCEIQVSYA 206          
BLAST of S-adenosylmethionine synthase vs. Ensembl Medaka
Match: ENSORLT00000011581.2 (S-adenosylmethionine synthase [Source:NCBI gene;Acc:101171015])

HSP 1 Score: 587.8 bits (1514), Expect = 0.000e+0
Identity = 286/380 (75.26%), Postives = 338/380 (88.95%), Query Frame = 3
Query:  135 NTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            + FLFTSESVGEGHPDKICDQISDA+LDAHL QDP+AKVACET AKTGMIL+ GEI+S+A VDYQKVVR+ I++IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVHL+R  +++GAGDQGLMFGYATDET ECMPLT+VLAH LN +++E RR+GTLPWLRPD+KTQVTV+YK D GA +PLRVHT+VIS QHDE +  + +++CL ++++K V+P  YLD+ETIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVK+ LCRRVLVQVSYAIGV+HPLS+ +  YGTS  S+ ELL IV+ NFDLRPG IV+EL+L+ PIY++TA YGHFGR+ F+WE PKKLK+
Sbjct:   13 DCFLFTSESVGEGHPDKICDQISDAVLDAHLSQDPDAKVACETVAKTGMILLAGEITSRAKVDYQKVVRDAIRDIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHLDRKEEDVGAGDQGLMFGYATDETDECMPLTIVLAHKLNAKMAELRRDGTLPWLRPDSKTQVTVQYKQDRGAVVPLRVHTIVISVQHDECVCLEEMRRCLKEQVVKAVVPSGYLDDETIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKSNLCRRVLVQVSYAIGVSHPLSVSIFHYGTSQRSERELLDIVMKNFDLRPGVIVRELDLKKPIYQRTAAYGHFGRDVFSWEMPKKLKY 392          
BLAST of S-adenosylmethionine synthase vs. Ensembl Medaka
Match: mat2ab (S-adenosylmethionine synthase [Source:NCBI gene;Acc:101160913])

HSP 1 Score: 586.645 bits (1511), Expect = 0.000e+0
Identity = 291/392 (74.23%), Postives = 340/392 (86.73%), Query Frame = 3
Query:  114 KMNGSSS-----NTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            +MNG  +     + FLFTSESVGEGHPDKICDQISDA+LDAHLKQDP+AKVACET AKTGMIL+ GE++S+A VDYQKVVR+TI++IGYD   KGFDYKTCNVL+A+E+QSPDIAQGVH++R  + IGAGDQGLMFGYATDET ECMPLT+VLAH LN +++E RRNGTLPWLRPD+KTQVTV+Y+ D GA +P+RVHT+V+S QHDE I  + ++  L +++IK V+P  YLD++TIYHL PSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSI +  YGTS  S+ ELL IV  NFDLRPG IV++L+L+ P Y+KTA YGHFGR+ F WE PKKLK+
Sbjct:    5 QMNGFHNSLIEEDCFLFTSESVGEGHPDKICDQISDAVLDAHLKQDPDAKVACETVAKTGMILLAGEVTSRATVDYQKVVRDTIRQIGYDDSSKGFDYKTCNVLVALEQQSPDIAQGVHIDRSEEEIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKMAELRRNGTLPWLRPDSKTQVTVQYRQDRGAMLPVRVHTIVVSVQHDEDICLEEMRDALKEKVIKAVVPCVYLDDDTIYHLQPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSISIFHYGTSKKSERELLDIVRKNFDLRPGVIVRDLDLKKPFYQKTAAYGHFGRDSFPWEVPKKLKY 396          
BLAST of S-adenosylmethionine synthase vs. Ensembl Medaka
Match: mat1a (S-adenosylmethionine synthase [Source:NCBI gene;Acc:101160342])

HSP 1 Score: 578.17 bits (1489), Expect = 0.000e+0
Identity = 284/380 (74.74%), Postives = 336/380 (88.42%), Query Frame = 3
Query:  135 NTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            N+F+FTSESVGEGHPDKICDQISDA+LDAHLKQDP+AKVACET  KTGM+L+ GEI+S+A VDYQKVVR+TIK IGYD+ +KGFDYKTCNVL+A+E+QSPDIAQGVH++R  ++IGAGDQGLMFGYATDET ECMPLT+VLAH LN +++E RRNGT+PWLRPD+KTQVTV Y+  +GA IP RVHT+VIS QHD+ ++ +  Q+ L +++IK V+P  YLD++TI HL PSGRFVIGGPQGDAG+TGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAAYAARWVAKSLVKA LCRRVLVQVSYAIGVA PLSI + +YG+S  +++ELL+IV  NFDLRPG IV++L L+ PIY++TA YGHFGR EF+WE PKKL F
Sbjct:    8 NSFMFTSESVGEGHPDKICDQISDAVLDAHLKQDPDAKVACETVCKTGMVLLCGEITSRANVDYQKVVRDTIKHIGYDNSDKGFDYKTCNVLVALEQQSPDIAQGVHVDRQEEDIGAGDQGLMFGYATDETEECMPLTIVLAHKLNAKMAELRRNGTVPWLRPDSKTQVTVHYEQKDGAVIPKRVHTIVISVQHDDGVTLEEQQKALKEKVIKAVVPAKYLDDKTICHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAAYAARWVAKSLVKAKLCRRVLVQVSYAIGVAQPLSISLFTYGSSKKTESELLKIVNKNFDLRPGVIVRDLQLKRPIYQQTAAYGHFGRSEFSWEVPKKLVF 387          
BLAST of S-adenosylmethionine synthase vs. Ensembl Medaka
Match: ENSORLT00000016106.2 (S-adenosylmethionine synthase [Source:NCBI gene;Acc:101159865])

HSP 1 Score: 572.778 bits (1475), Expect = 0.000e+0
Identity = 285/397 (71.79%), Postives = 334/397 (84.13%), Query Frame = 3
Query:  111 IKMNGS---------SSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            ++MNGS             FLFTSESVGEGHPDK+CDQISDA+LDAHL QDP+AKVACET  KTGM+L+ GEI+S+A VDYQKVVR+ IK IGYD   KG DYKTCNVL+A+EEQSPDIAQGVH++R    IGAGDQGLMFGYATDET ECMPLT+VLAH LN R++E RRNGT+PWLRPD+KTQVTV Y  ++GA IP R+HTVVIS QHD+ +S +  +  L++++I+ VIP  YLDE T+YHL PSGRFVIGGPQGDAG+TGRKIIVDTYGGWGAHGGGAFSGKD++KVDRSAAYAARWVAKSLV A LCRRVLVQV+YAIGVAHPLSI + ++G+S  S+ ELL+IV  NFDLRPG IV++L+L+ PIY+KTACYGHFGR+EF WE PK L+F
Sbjct:    1 MEMNGSVDGSCERAPDEGAFLFTSESVGEGHPDKMCDQISDAVLDAHLSQDPDAKVACETVCKTGMVLLCGEITSRASVDYQKVVRDVIKHIGYDSSSKGLDYKTCNVLVALEEQSPDIAQGVHVDRMESEIGAGDQGLMFGYATDETEECMPLTIVLAHKLNARMAELRRNGTVPWLRPDSKTQVTVHYSQESGAVIPERIHTVVISVQHDDHVSLEEQRSVLMEKVIRAVIPAKYLDENTVYHLQPSGRFVIGGPQGDAGVTGRKIIVDTYGGWGAHGGGAFSGKDYTKVDRSAAYAARWVAKSLVNARLCRRVLVQVAYAIGVAHPLSISLFTFGSSQKSEKELLQIVYRNFDLRPGVIVRDLDLKRPIYQKTACYGHFGRKEFPWEVPKNLEF 397          
BLAST of S-adenosylmethionine synthase vs. Ensembl Medaka
Match: mat2al (S-adenosylmethionine synthase [Source:NCBI gene;Acc:101170772])

HSP 1 Score: 533.872 bits (1374), Expect = 0.000e+0
Identity = 270/390 (69.23%), Postives = 318/390 (81.54%), Query Frame = 3
Query:  117 MNGSSSN----TFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            MN S  +    TFLFTSESVGEGH DK+CDQISDA+LDA+L QDP++KVACE  AKTGMIL+ GE++S+A VD Q VVR TIK+IGYD   KGFDYKTCNVL+A+E Q+ +I+  V   R+ ++IGAGDQGLMFGYATDET ECMPLT++LAH LN ++ E  RNG  PW+ PD K+QVTVEY+ D GA  PLRVHTVVIS QH   IS + ++  L+++++K VIP  YLD++T+YHL PSG+F++GGPQGDAGLTGRKIIVDTYGGWG HGGGAFSGKD+SKVDRS AYAARWVAKSLVKAGLCRR LVQ+SY+I V+HPLSI V  YGTSS  + ELL+IV DNFDLRPG IV EL L+ PIY+ TACYGHFGREEF WEKPK L F
Sbjct:    1 MNASGGSRGGKTFLFTSESVGEGHSDKMCDQISDAVLDAYLSQDPDSKVACECVAKTGMILLVGEVTSRAKVDLQAVVRNTIKKIGYDSSSKGFDYKTCNVLVALEPQAQEISDCVFEGRNQEDIGAGDQGLMFGYATDETEECMPLTILLAHKLNHKMKELSRNGDCPWILPDCKSQVTVEYRDDAGAMEPLRVHTVVISVQHSPDISLEEIRHNLMEKVVKAVIPAKYLDDQTVYHLLPSGKFLLGGPQGDAGLTGRKIIVDTYGGWGGHGGGAFSGKDYSKVDRSGAYAARWVAKSLVKAGLCRRALVQISYSIAVSHPLSITVFHYGTSSRDEDELLQIVKDNFDLRPGVIVTELGLKRPIYQATACYGHFGREEFPWEKPKPLVF 390          
BLAST of S-adenosylmethionine synthase vs. Planmine SMEST
Match: SMESG000027587.1 (SMESG000027587.1)

HSP 1 Score: 770.385 bits (1988), Expect = 0.000e+0
Identity = 386/386 (100.00%), Postives = 386/386 (100.00%), Query Frame = 3
Query:  117 MNGSSSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            MNGSSSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF
Sbjct:    1 MNGSSSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 386          
BLAST of S-adenosylmethionine synthase vs. Planmine SMEST
Match: SMESG000053202.1 (SMESG000053202.1)

HSP 1 Score: 616.305 bits (1588), Expect = 0.000e+0
Identity = 301/379 (79.42%), Postives = 343/379 (90.50%), Query Frame = 3
Query:  138 TFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            TFLFTSESVGEGHPDKICDQ+SDA+LDAHL QDP AKVACETF+KTGMIL+GGEI+S A+V+YQ+V+R+TIK+IG+D CEKGFDYKTCN+L+AIE+Q+P+IAQ VH ER  ++IGAGDQGLMFGYATDET ECMPLTVVLAH LN +L+ECRRN TLP+LRPDTKTQVTV YKS NGACIPL+VHT+VISTQHDE I    L++ L++ ++K VIPE YLD  TIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD+SKVDRS AYAARWVAKSLVKAGLCRRVLVQ+SYAIG+A PLSI+VDSYGT S  D++LL I+ DNFDLRPGHI K+L+LR PIY+KTACYGHFGR EFTWE+PK LK+
Sbjct:   15 TFLFTSESVGEGHPDKICDQVSDAVLDAHLSQDPKAKVACETFSKTGMILIGGEITSNAIVNYQRVIRQTIKDIGFDSCEKGFDYKTCNLLIAIEQQNPEIAQSVHEERSDEDIGAGDQGLMFGYATDETEECMPLTVVLAHGLNMKLAECRRNNTLPYLRPDTKTQVTVLYKSVNGACIPLKVHTIVISTQHDENIHINQLRKDLMEHVVKTVIPEKYLDASTIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDYSKVDRSGAYAARWVAKSLVKAGLCRRVLVQLSYAIGIAAPLSIFVDSYGTGSIPDSQLLEIINDNFDLRPGHIEKDLDLRRPIYQKTACYGHFGRPEFTWEQPKTLKY 393          
BLAST of S-adenosylmethionine synthase vs. Planmine SMEST
Match: SMESG000013953.1 (SMESG000013953.1)

HSP 1 Score: 562.762 bits (1449), Expect = 0.000e+0
Identity = 278/381 (72.97%), Postives = 322/381 (84.51%), Query Frame = 3
Query:  132 SNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETFAKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLAIEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHALNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQHDETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYXXXXXXXXXXXXXKDFSKVDRSAAYAARWVAKSLVKAGLCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGHIVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF 1274
            ++ FLFTSESVGEGHPDKICDQISDAILDAHLKQDP AKVACE+F KTGM+LVGGEI+S AVV+Y  +VR TIK IG+D  EKG DY+TCNVL+AIE+QSPDIAQGV++ R  D+IGAGDQGLMFGYATDET ECMP+T++LAH LN+RL+ CRRN  L WLRPDTKTQVT+ YK+ +G  +PL VHT+VIS QHDE ++ ++++  L+K +I  VI    L   TIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKD +KVDRSAAYAARWVAKSLVK GLC+RVLVQ SYAIG+A PLSI+V SY +S+ +D ELL IV  NFDLRPG I+++L LR PI++KTA YGHFGR EF WE PKKL F
Sbjct:    4 NDIFLFTSESVGEGHPDKICDQISDAILDAHLKQDPYAKVACESFTKTGMVLVGGEITSHAVVNYSDIVRNTIKRIGFDSSEKGLDYRTCNVLVAIEQQSPDIAQGVYVNRSEDSIGAGDQGLMFGYATDETEECMPMTLILAHKLNQRLAYCRRNDILGWLRPDTKTQVTIAYKNKDGGLLPLYVHTIVISAQHDEEVTLENIKSDLMKHVISNVIDSKMLTSSTIYHLNPSGRFVIGGPQGDAGLTGRKIIVDTYGGWGAHGGGAFSGKDATKVDRSAAYAARWVAKSLVKNGLCKRVLVQFSYAIGIAAPLSIFVYSYNSSTKTDNELLAIVNQNFDLRPGAIIRDLKLREPIFEKTASYGHFGRNEFLWENPKKLHF 384          
The following BLAST results are available for this feature:
BLAST of S-adenosylmethionine synthase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
MAT2A0.000e+076.32methionine adenosyltransferase 2A [Source:HGNC Sym... [more]
MAT1A0.000e+073.56methionine adenosyltransferase 1A [Source:HGNC Sym... [more]
MAT2A3.843e-15675.59methionine adenosyltransferase 2A [Source:HGNC Sym... [more]
MAT1A6.750e-7560.75methionine adenosyltransferase 1A [Source:HGNC Sym... [more]
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BLAST of S-adenosylmethionine synthase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sams-30.000e+070.94Probable S-adenosylmethionine synthase 3 [Source:... [more]
sams-40.000e+071.69Probable S-adenosylmethionine synthase 4 [Source:... [more]
sams-40.000e+071.69Probable S-adenosylmethionine synthase 4 [Source:... [more]
sams-50.000e+070.83Probable S-adenosylmethionine synthase 5 [Source:... [more]
sams-50.000e+070.83Probable S-adenosylmethionine synthase 5 [Source:... [more]
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BLAST of S-adenosylmethionine synthase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Sam-S0.000e+070.48gene:FBgn0005278 transcript:FBtr0089430[more]
Sam-S0.000e+070.48gene:FBgn0005278 transcript:FBtr0089433[more]
Sam-S0.000e+070.48gene:FBgn0005278 transcript:FBtr0089432[more]
Sam-S0.000e+070.48gene:FBgn0005278 transcript:FBtr0089429[more]
Sam-S0.000e+070.48gene:FBgn0005278 transcript:FBtr0089434[more]
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BLAST of S-adenosylmethionine synthase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mat2aa0.000e+075.79methionine adenosyltransferase II, alpha a [Source... [more]
mat1a0.000e+075.40methionine adenosyltransferase I, alpha [Source:ZF... [more]
mat1a0.000e+075.40methionine adenosyltransferase I, alpha [Source:ZF... [more]
mat2ab0.000e+077.52methionine adenosyltransferase II, alpha b [Source... [more]
mat2al0.000e+068.59methionine adenosyltransferase II, alpha-like [Sou... [more]
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BLAST of S-adenosylmethionine synthase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
use10.000e+073.48unconventional SNARE in the ER 1 homolog [Source:X... [more]
use10.000e+075.53unconventional SNARE in the ER 1 homolog [Source:X... [more]
mat1a0.000e+075.93methionine adenosyltransferase I, alpha [Source:NC... [more]
use10.000e+072.05unconventional SNARE in the ER 1 homolog [Source:X... [more]
use10.000e+075.07unconventional SNARE in the ER 1 homolog [Source:X... [more]
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BLAST of S-adenosylmethionine synthase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Mat2a0.000e+076.32methionine adenosyltransferase II, alpha [Source:M... [more]
Mat2a0.000e+076.32methionine adenosyltransferase II, alpha [Source:M... [more]
Mat1a0.000e+074.41methionine adenosyltransferase I, alpha [Source:MG... [more]
Mat1a0.000e+074.41methionine adenosyltransferase I, alpha [Source:MG... [more]
Mat2a0.000e+077.14methionine adenosyltransferase II, alpha [Source:M... [more]
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BLAST of S-adenosylmethionine synthase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P18298|METK2_RAT0.000e+076.32S-adenosylmethionine synthase isoform type-2 OS=Ra... [more]
sp|Q5R5H1|METK2_PONAB0.000e+076.32S-adenosylmethionine synthase isoform type-2 OS=Po... [more]
sp|Q4R924|METK2_MACFA0.000e+076.32S-adenosylmethionine synthase isoform type-2 OS=Ma... [more]
sp|P31153|METK2_HUMAN0.000e+076.32S-adenosylmethionine synthase isoform type-2 OS=Ho... [more]
sp|Q3THS6|METK2_MOUSE0.000e+076.32S-adenosylmethionine synthase isoform type-2 OS=Mu... [more]
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BLAST of S-adenosylmethionine synthase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8I6050.000e+078.44S-adenosylmethionine synthase OS=Macrostomum ligna... [more]
A0A267DP280.000e+078.44S-adenosylmethionine synthase OS=Macrostomum ligna... [more]
A0A1I8HKQ40.000e+078.18S-adenosylmethionine synthase OS=Macrostomum ligna... [more]
A0A267H2L80.000e+077.34S-adenosylmethionine synthase OS=Macrostomum ligna... [more]
A0A267EMZ10.000e+077.08S-adenosylmethionine synthase OS=Macrostomum ligna... [more]
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BLAST of S-adenosylmethionine synthase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mat2ab0.000e+073.47S-adenosylmethionine synthase [Source:NCBI gene;Ac... [more]
mat1a0.000e+075.20S-adenosylmethionine synthase [Source:NCBI gene;Ac... [more]
mat1a0.000e+073.08S-adenosylmethionine synthase [Source:NCBI gene;Ac... [more]
mat2aa0.000e+075.79S-adenosylmethionine synthase [Source:NCBI gene;Ac... [more]
mat2al3.952e-17667.36S-adenosylmethionine synthase [Source:NCBI gene;Ac... [more]
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BLAST of S-adenosylmethionine synthase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of S-adenosylmethionine synthase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
SAM20.000e+069.97S-adenosylmethionine synthetase; catalyzes transfe... [more]
SAM10.000e+070.08S-adenosylmethionine synthetase; catalyzes transfe... [more]
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BLAST of S-adenosylmethionine synthase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 4
Match NameE-valueIdentityDescription
EDO378500.000e+073.63S-adenosylmethionine synthase [Source:UniProtKB/T... [more]
EDO378250.000e+072.47S-adenosylmethionine synthase [Source:UniProtKB/T... [more]
EDO451172.212e-17767.89S-adenosylmethionine synthase [Source:UniProtKB/T... [more]
EDO268701.776e-7060.38Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of S-adenosylmethionine synthase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000011581.20.000e+075.26S-adenosylmethionine synthase [Source:NCBI gene;Ac... [more]
mat2ab0.000e+074.23S-adenosylmethionine synthase [Source:NCBI gene;Ac... [more]
mat1a0.000e+074.74S-adenosylmethionine synthase [Source:NCBI gene;Ac... [more]
ENSORLT00000016106.20.000e+071.79S-adenosylmethionine synthase [Source:NCBI gene;Ac... [more]
mat2al0.000e+069.23S-adenosylmethionine synthase [Source:NCBI gene;Ac... [more]
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BLAST of S-adenosylmethionine synthase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000027587.10.000e+0100.00SMESG000027587.1[more]
SMESG000053202.10.000e+079.42SMESG000053202.1[more]
SMESG000013953.10.000e+072.97SMESG000013953.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027707 ID=SMED30027707|Name=S-adenosylmethionine synthase|organism=Schmidtea mediterranea sexual|type=transcript|length=1648bp
TGGAGAATTTTAAACAATATCATACGAAGCTTAAATCAAAATTAACAATT
TTGCAACTATAAATACGAACTGCTTTCAGAAATAATTTGTCTCTTACAGT
TTTGTAAAAAATAAAAATGAATGGTTCTTCTTCCAATACATTTTTATTTA
CTTCTGAATCAGTTGGTGAAGGCCATCCAGATAAAATCTGTGATCAAATT
AGTGATGCCATTCTAGATGCACATTTAAAACAAGATCCAAACGCTAAAGT
TGCCTGTGAAACTTTTGCTAAAACTGGTATGATATTAGTCGGAGGAGAAA
TCTCATCTAAGGCGGTAGTTGATTATCAAAAAGTTGTCAGAGAAACTATT
AAAGAAATTGGATATGATCATTGCGAAAAAGGATTTGATTATAAAACATG
CAATGTTCTTCTAGCCATTGAAGAACAAAGTCCAGATATTGCTCAGGGTG
TTCACTTGGAACGAGACTTCGACAATATTGGAGCTGGAGATCAAGGACTT
ATGTTCGGTTATGCTACTGATGAAACCGCAGAATGCATGCCTTTAACAGT
AGTTCTTGCTCACGCTTTGAATAAGCGATTATCAGAGTGCAGAAGAAATG
GGACTTTACCTTGGTTGCGACCAGATACTAAAACACAAGTGACAGTGGAA
TATAAATCAGACAATGGAGCATGTATCCCTCTCAGAGTTCACACGGTTGT
CATTTCAACTCAGCACGATGAAACCATCAGTCATCAACATTTGCAGCAAT
GTCTTATCAAAGAAATTATTAAAAAAGTCATTCCAGAGCATTATCTCGAT
GAAGAAACAATTTACCACCTCAACCCAAGTGGAAGATTTGTTATCGGTGG
TCCCCAAGGCGACGCAGGACTGACTGGTCGGAAAATAATTGTGGACACTT
ATGGCGGCTGGGGAGCACATGGAGGGGGAGCGTTTTCTGGGAAAGATTTC
TCCAAAGTCGACAGAAGTGCTGCATATGCTGCAAGATGGGTGGCAAAATC
TTTGGTCAAAGCTGGCCTTTGTCGTCGGGTTTTGGTACAGGTTTCGTACG
CCATTGGAGTCGCACATCCCCTTTCAATTTATGTTGACAGTTACGGAACT
TCCTCTCATTCAGATGCTGAACTGTTGCGGATTGTCGTTGACAATTTTGA
TTTGCGGCCTGGACACATTGTCAAAGAGCTCAATTTGCGGCACCCAATAT
ACAAGAAGACCGCTTGCTATGGACATTTTGGGCGGGAGGAATTTACTTGG
GAAAAACCGAAGAAACTGAAATTTTAAAAGATTCCCTGTTGATAATAAAT
TGTTTCTATGTCGAGATGTGCATCATTTATTGACTTCTATTTTAGTGTAT
AAATATCCTTGACATAGAATTTGCTTTTATGAATAACGTTCAGCAAGTGC
CTCAAGAAATTTGTGATAGATATAATATTTTTTGTCAATGAATAAATTGG
TTTTCTTAGTTATTTCGTCAATTTTACACTGGTATATGACATCAGTGTAA
AGCAGTTTTTAATTGGCCGAATGTTTTGATTGGTTGGCTTATTGTGTAAT
ACGAATGCTTTTGTGAGTCATTGATGCAAATATTGGCTTATTTATTTTAT
CCATCGCAAGCTTTGTTTTGTTGAAATATTAAAAAAACTCCCAGCCAC
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protein sequence of SMED30027707-orf-1

>SMED30027707-orf-1 ID=SMED30027707-orf-1|Name=SMED30027707-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=387bp
MNGSSSNTFLFTSESVGEGHPDKICDQISDAILDAHLKQDPNAKVACETF
AKTGMILVGGEISSKAVVDYQKVVRETIKEIGYDHCEKGFDYKTCNVLLA
IEEQSPDIAQGVHLERDFDNIGAGDQGLMFGYATDETAECMPLTVVLAHA
LNKRLSECRRNGTLPWLRPDTKTQVTVEYKSDNGACIPLRVHTVVISTQH
DETISHQHLQQCLIKEIIKKVIPEHYLDEETIYHLNPSGRFVIGGPQGDA
GLTGRKIIVDTYGGWGAHGGGAFSGKDFSKVDRSAAYAARWVAKSLVKAG
LCRRVLVQVSYAIGVAHPLSIYVDSYGTSSHSDAELLRIVVDNFDLRPGH
IVKELNLRHPIYKKTACYGHFGREEFTWEKPKKLKF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000012neural progenitor cell
PLANA:0000016pharynx
PLANA:0000028Category 2 cell
PLANA:0000034epidermis
PLANA:0000054dorsal region of the epidermis
PLANA:0000418head
PLANA:0000429neoblast
PLANA:0000484secretory system
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR002133S-AdoMet_synthetase
IPR022630S-AdoMet_synt_C
IPR022629S-AdoMet_synt_central
IPR022628S-AdoMet_synt_N
IPR022631ADOMET_SYNTHASE_CS
IPR022636S-AdoMet_synthetase_sfam
Vocabulary: molecular function
TermDefinition
GO:0004478methionine adenosyltransferase activity
GO:0005524ATP binding
GO:0016740transferase activity
GO:0046872metal ion binding
GO:0000166nucleotide binding
Vocabulary: biological process
TermDefinition
GO:0006556S-adenosylmethionine biosynthetic process
GO:0006730one-carbon metabolic process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022630S-adenosylmethionine synthetase, C-terminalPFAMPF02773S-AdoMet_synt_Ccoord: 243..380
e-value: 4.2E-69
score: 230.9
IPR022629S-adenosylmethionine synthetase, central domainPFAMPF02772S-AdoMet_synt_Mcoord: 121..241
e-value: 3.2E-47
score: 159.7
IPR002133S-adenosylmethionine synthetaseTIGRFAMTIGR01034TIGR01034coord: 10..385
e-value: 6.2E-189
score: 625.6
IPR002133S-adenosylmethionine synthetasePIRSFPIRSF000497MATcoord: 3..386
e-value: 1.9E-172
score: 571.1
IPR002133S-adenosylmethionine synthetasePANTHERPTHR11964S-ADENOSYLMETHIONINE SYNTHETASEcoord: 6..386
NoneNo IPR availableGENE3DG3DSA:3.30.300.10coord: 120..379
e-value: 3.8E-178
score: 594.1
NoneNo IPR availableGENE3DG3DSA:3.30.300.10coord: 10..245
e-value: 3.8E-178
score: 594.1
NoneNo IPR availableGENE3DG3DSA:3.30.300.10coord: 16..282
e-value: 3.8E-178
score: 594.1
NoneNo IPR availablePANTHERPTHR11964:SF12S-ADENOSYLMETHIONINE SYNTHASE ISOFORM TYPE-2coord: 6..386
NoneNo IPR availableCDDcd18079S-AdoMet_syntcoord: 10..382
e-value: 0.0
score: 716.49
IPR022628S-adenosylmethionine synthetase, N-terminalPFAMPF00438S-AdoMet_synt_Ncoord: 9..106
e-value: 2.0E-44
score: 150.3
IPR022631S-adenosylmethionine synthetase, conserved sitePROSITEPS00377ADOMET_SYNTHASE_2coord: 269..277
IPR022631S-adenosylmethionine synthetase, conserved sitePROSITEPS00376ADOMET_SYNTHASE_1coord: 122..132
IPR022636S-adenosylmethionine synthetase superfamilySUPERFAMILYSSF55973S-adenosylmethionine synthetasecoord: 118..242
IPR022636S-adenosylmethionine synthetase superfamilySUPERFAMILYSSF55973S-adenosylmethionine synthetasecoord: 243..385
IPR022636S-adenosylmethionine synthetase superfamilySUPERFAMILYSSF55973S-adenosylmethionine synthetasecoord: 8..114