General transcription factor IIF subunit 2

Overview
NameGeneral transcription factor IIF subunit 2
Smed IDSMED30027693
Length (bp)1122
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of General transcription factor IIF subunit 2 (SMED30027693) t-SNE clustered cells

Violin plots show distribution of expression levels for General transcription factor IIF subunit 2 (SMED30027693) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of General transcription factor IIF subunit 2 (SMED30027693) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for General transcription factor IIF subunit 2 (SMED30027693) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30027693

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30027693h1SMcG0019262 dd_Smed_v4_2142_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30027693h1SMcG0019262 dd_Smed_v4_2142_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30027693h1SMcG0019262 dd_Smed_v4_2142_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Human
Match: GTF2F2 (general transcription factor IIF subunit 2 [Source:HGNC Symbol;Acc:HGNC:4653])

HSP 1 Score: 119.013 bits (297), Expect = 1.084e-30
Identity = 77/248 (31.05%), Postives = 120/248 (48.39%), Query Frame = 1 
Query:  112 VWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQ-GVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            VWLVK+P YLS +W  +     V K+ + + + +  V        F  N+D+  ++   I G    V+   E      S+G +    F   SSD              K ++ G V  R    P     Y  LK  QI   + P+R +Q  ++     +KP +    N + +   ++  + AR +   ++D++ S FEKHQ+YN+KDL+D+T QP  Y+KEILKE+   +     ++ WELKPEYRHY
Sbjct:   18 VWLVKVPKYLSQQWAKASGRGEVGKLRIAKTQGRTEV-------SFTLNEDLANIHD--IGGKPASVSAPREHPFVLQSVGGQTLTVFTESSSD--------------KLSLEGIVVQRAECRPAASENYMRLKRLQIEESSKPVRLSQQLDKVVTTNYKPVANHQYNIEYERKKKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVVYLKEILKEIGVQNVKGIHKNTWELKPEYRHY 242
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Celegans
Match: gtf-2F2 (General transcription factor IIF subunit 2 [Source:UniProtKB/TrEMBL;Acc:Q8MYM8])

HSP 1 Score: 97.4413 bits (241), Expect = 4.537e-23
Identity = 76/271 (28.04%), Postives = 122/271 (45.02%), Query Frame = 1 
Query:   85 VDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLI---------------CNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            VD       +WLVK+P YLS  W ++ + ++V K+ +    +      LI                 P+     +IPT   FI+  +          +++ +S+ S D K  +   + I+ G          K AI GR+  +    PP  + Y  +K   I     P +  ++ E+  V+FKP S    +  K    +DG ++ R +   L   +   FEKH FY ++DL  L  QP  YVKE+L+E+A  + AP  + +W LKPEY +Y
Sbjct:   11 VDCELAKRSIWLVKVPRYLSELW-DANEGNVVGKLQIGAQVELHTAKGLIHPKPKEPTEGSSSTVTQPE---ASEIPTEYSFILHDV----------KSQTMSVLSED-KQGLGADAPIKTG----------KLAIEGRIIKKAECRPPATSKYMKMKLAHIVKNTQPKKTVKMIEKAAVKFKPVSVHAEDMIKSKQKKDGAKTYRADRDVLRGALFKAFEKHSFYRLQDLQQLLQQPVSYVKEVLQEIAVYNTAPPHKSLWCLKPEYCNY 256
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Fly
Match: TfIIFbeta (gene:FBgn0010421 transcript:FBtr0082310)

HSP 1 Score: 124.405 bits (311), Expect = 8.900e-33
Identity = 81/260 (31.15%), Postives = 128/260 (49.23%), Query Frame = 1 
Query:   85 VDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIK---NKDIPTVNKFIIQGITEK-VNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            +D S     VWLVK+P Y++ +W  +P N  V K+ +++   ++A   L   P  +     + IPT +   +  +T++ + V S        +   DGK         E       +P  +K  + GR+  +    P  DN Y  LK + IR  + P R+ Q  ++    FKP      N +     +   + AR + + +MD++   FEKHQ+YNIKDL+ +T QP  Y+KEILK+V   +     ++MWELK EYRHY
Sbjct:   16 LDLSNAGRGVWLVKVPKYIAQKWEKAPTNMDVGKLRINKTPGQKAQVSLSLTPAVLALDPEEKIPTEHILDVSQVTKQTLGVFSH-------MAPSDGK---------ENSTTSAAQPDNEKLYMEGRIVQKLECRPIADNCYMKLKLESIRKASEPQRRVQPIDKIVQNFKPVKDHAHNIEYRERKKAEGKKARDDKNAVMDMLFHAFEKHQYYNIKDLVKITNQPISYLKEILKDVCDYNMKNPHKNMWELKKEYRHY 259
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Zebrafish
Match: gtf2f2a (general transcription factor IIF, polypeptide 2a [Source:ZFIN;Acc:ZDB-GENE-040625-131])

HSP 1 Score: 131.339 bits (329), Expect = 2.062e-35
Identity = 82/255 (32.16%), Postives = 129/255 (50.59%), Query Frame = 1 
Query:  112 VWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFIL-------QSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQ-GVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            VWLVK+P YLS +W  +     V K+ + +++ K  V        F  N+++ T     I+ I EK ++    R    ++ +  G+T  +       QS     G   G  P   K A+ G V  R    P    +Y  LK  QI   + PLR +Q  ++     +KP +    N   +   ++  + AR +  +++D++ S FEKHQFYNIKDL+D+T QP  Y+KEIL+++   +   + ++ WELKPEYRHY
Sbjct:   18 VWLVKVPKYLSQQWTKATGRGEVGKLRISKNQGKAEV-------SFTLNEELTT-----IETIGEKTSMVRAPREHPFTLQTVGGQTLAVFTENSSGQSDAEASGSGTGSGP--DKIALEGVVVQRAECRPAVSESYMRLKKLQIEELSKPLRFSQQLDKAVTTNYKPVANHAYNLDYEKRKKEEGKRARADKQQVLDMLFSAFEKHQFYNIKDLVDITKQPVIYLKEILRDIGIYNVKGTHKNTWELKPEYRHY 258
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Zebrafish
Match: gtf2f2a (general transcription factor IIF, polypeptide 2a [Source:ZFIN;Acc:ZDB-GENE-040625-131])

HSP 1 Score: 128.642 bits (322), Expect = 1.716e-34
Identity = 81/251 (32.27%), Postives = 127/251 (50.60%), Query Frame = 1 
Query:  112 VWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKT---FILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQ-GVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            VWLVK+P YLS +W  +     V K+ + +++ K  V        F  N+++ T     I+ I EK ++    R    ++ +  G+T   F   SSD              K A+ G V  R    P    +Y  LK  QI   + PLR +Q  ++     +KP +    N   +   ++  + AR +  +++D++ S FEKHQFYNIKDL+D+T QP  Y+KEIL+++   +   + ++ WELKPEYRHY
Sbjct:   18 VWLVKVPKYLSQQWTKATGRGEVGKLRISKNQGKAEV-------SFTLNEELTT-----IETIGEKTSMVRAPREHPFTLQTVGGQTLAVFTENSSD--------------KIALEGVVVQRAECRPAVSESYMRLKKLQIEELSKPLRFSQQLDKAVTTNYKPVANHAYNLDYEKRKKEEGKRARADKQQVLDMLFSAFEKHQFYNIKDLVDITKQPVIYLKEILRDIGIYNVKGTHKNTWELKPEYRHY 242
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Zebrafish
Match: gtf2f2a (general transcription factor IIF, polypeptide 2a [Source:ZFIN;Acc:ZDB-GENE-040625-131])

HSP 1 Score: 128.642 bits (322), Expect = 1.716e-34
Identity = 81/251 (32.27%), Postives = 127/251 (50.60%), Query Frame = 1 
Query:  112 VWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKT---FILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQ-GVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            VWLVK+P YLS +W  +     V K+ + +++ K  V        F  N+++ T     I+ I EK ++    R    ++ +  G+T   F   SSD              K A+ G V  R    P    +Y  LK  QI   + PLR +Q  ++     +KP +    N   +   ++  + AR +  +++D++ S FEKHQFYNIKDL+D+T QP  Y+KEIL+++   +   + ++ WELKPEYRHY
Sbjct:   18 VWLVKVPKYLSQQWTKATGRGEVGKLRISKNQGKAEV-------SFTLNEELTT-----IETIGEKTSMVRAPREHPFTLQTVGGQTLAVFTENSSD--------------KIALEGVVVQRAECRPAVSESYMRLKKLQIEELSKPLRFSQQLDKAVTTNYKPVANHAYNLDYEKRKKEEGKRARADKQQVLDMLFSAFEKHQFYNIKDLVDITKQPVIYLKEILRDIGIYNVKGTHKNTWELKPEYRHY 242
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Zebrafish
Match: gtf2f2b (general transcription factor IIF, polypeptide 2b [Source:ZFIN;Acc:ZDB-GENE-050417-374])

HSP 1 Score: 123.635 bits (309), Expect = 1.178e-32
Identity = 84/267 (31.46%), Postives = 132/267 (49.44%), Query Frame = 1 
Query:   67 DDAVYSVDASQC--DDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFIL--QSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQ-GVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
             D  + VD ++   +  VWLVK+P YLS +W  +     V K+ + + + K  V        F  N+++  V+     G  EK++     R    ++ +  G+T  +  +SSD              K A+ G V  R    P    +Y  LK  QI     PLR +Q  E+     +KP S  + N + +   ++  + AR +  K+++++ S FEKHQFYNI+DL+D+T QP  Y+KEIL+E+   +     +  WELKPEYRHY
Sbjct:    2 SDKGHDVDLTRAKQNTGVWLVKVPKYLSQQWAKASGRGEVGKLKIGKAQGKTVV-------SFNLNEELTVVD---CSG--EKISSVRSPREHPFTMQTVGGQTLAVFTESSD--------------KIALEGMVVQRAECRPAVSESYMKLKKLQIEESTKPLRFSQRLEKAITTNYKPVSNHSHNLEYEKRKKEEGKRARADKQKVLEMLFSAFEKHQFYNIRDLVDITKQPVIYLKEILREIGVYNSRGPHKSTWELKPEYRHY 242
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Zebrafish
Match: gtf2f2a (general transcription factor IIF, polypeptide 2a [Source:ZFIN;Acc:ZDB-GENE-040625-131])

HSP 1 Score: 102.449 bits (254), Expect = 8.635e-26
Identity = 53/140 (37.86%), Postives = 81/140 (57.86%), Query Frame = 1 
Query:  436 KKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQ-GVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            K A+ G V  R    P    +Y  LK  QI   + PLR +Q  ++     +KP +    N   +   ++  + AR +  +++D++ S FEKHQFYNIKDL+D+T QP  Y+KEIL+++   +   + ++ WELKPEYRHY
Sbjct:    9 KIALEGVVVQRAECRPAVSESYMRLKKLQIEELSKPLRFSQQLDKAVTTNYKPVANHAYNLDYEKRKKEEGKRARADKQQVLDMLFSAFEKHQFYNIKDLVDITKQPVIYLKEILRDIGIYNVKGTHKNTWELKPEYRHY 148
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Xenopus
Match: gtf2f2 (mitogen-activated protein kinase 12 [Source:Xenbase;Acc:XB-GENE-956264])

HSP 1 Score: 125.176 bits (313), Expect = 6.829e-33
Identity = 75/258 (29.07%), Postives = 133/258 (51.55%), Query Frame = 1 
Query:   85 VDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRY-KKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQ-GVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            ++ ++ +  +WLVK+P YL+ +W  +     V K+ + +++ K  V        F  N+++ ++     +  +  V    E      S+G   G+T  + +  + G  +   + R   K A+ G V  R    P     Y  +K KQI   + P R++Q  E+     ++P S    N + +   +D  + AR +  +++D++ S FEKHQ+YNIKDL+D+T QP  Y+KEIL+++   +   + ++ WELKPEYRHY
Sbjct:   10 LNGAKQNTGMWLVKLPKYLAQQWAKATGRGEVGKLRIVKNQGKTEV-------SFTLNEELASIQDIGGKPASSLVTAPREHPFLLQSVG---GQTLTVFTESLSGQSDDKSENRVIDKLALEGIVVHRAECRPAASENYMQMKRKQIEESSKPKRQSQQIEKAVTTNYRPVSNHQYNIEYEKKKKDDGKRARADKHQVLDMLFSAFEKHQYYNIKDLVDITKQPVSYLKEILRDIGIYNMKGTHKNTWELKPEYRHY 257
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Xenopus
Match: gtf2f2 (mitogen-activated protein kinase 12 [Source:Xenbase;Acc:XB-GENE-956264])

HSP 1 Score: 122.865 bits (307), Expect = 3.571e-32
Identity = 74/257 (28.79%), Postives = 127/257 (49.42%), Query Frame = 1 
Query:   85 VDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQ-GVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            ++ ++ +  +WLVK+P YL+ +W  +     V K+ + +++ K  V        F  N+++ ++     +  +  V    E      S+G +    F    SD              K A+ G V  R    P     Y  +K KQI   + P R++Q  E+     ++P S    N + +   +D  + AR +  +++D++ S FEKHQ+YNIKDL+D+T QP  Y+KEIL+++   +   + ++ WELKPEYRHY
Sbjct:   10 LNGAKQNTGMWLVKLPKYLAQQWAKATGRGEVGKLRIVKNQGKTEV-------SFTLNEELASIQDIGGKPASSLVTAPREHPFLLQSVGGQTLTVFTESLSD--------------KLALEGIVVHRAECRPAASENYMQMKRKQIEESSKPKRQSQQIEKAVTTNYRPVSNHQYNIEYEKKKKDDGKRARADKHQVLDMLFSAFEKHQYYNIKDLVDITKQPVSYLKEILRDIGIYNMKGTHKNTWELKPEYRHY 245
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Mouse
Match: Gtf2f2 (general transcription factor IIF, polypeptide 2 [Source:MGI Symbol;Acc:MGI:1915955])

HSP 1 Score: 123.25 bits (308), Expect = 1.610e-32
Identity = 78/248 (31.45%), Postives = 122/248 (49.19%), Query Frame = 1 
Query:  112 VWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQ-GVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            VWLVK+P YLS +W  +     V K+ + +++ +  V        F  N+D+  ++   I G    V+   E      S+G +    F   SSD              K ++ G V  R    P     Y  LK  QI   + P+R +Q  ++     +KP +    N + +   ++  + AR +   ++D++ S FEKHQ+YN+KDL+D+T QP GY+KEILKE+   +     ++ WELKPEYRHY
Sbjct:   18 VWLVKVPKYLSQQWAKASGRGEVGKLRIAKNQGRTEV-------SFTLNEDLANIHD--IGGKPASVSAPREHPFVLQSVGGQTLTVFTESSSD--------------KLSLEGIVVQRAECRPAASENYMKLKRLQIEESSKPVRLSQQLDKVVTTNYKPVANHQYNIEYERKKKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVGYLKEILKEIGIQNVKGIHKNTWELKPEYRHY 242
[Close]
BLAST of General transcription factor IIF subunit 2 vs. UniProt/SwissProt
Match: sp|Q01750|T2FB_RAT (General transcription factor IIF subunit 2 OS=Rattus norvegicus OX=10116 GN=Gtf2f2 PE=1 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 5.292e-33
Identity = 79/248 (31.85%), Postives = 123/248 (49.60%), Query Frame = 1 
Query:  112 VWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQ-GVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            VWLVK+P YLS +W  +P    V K+ + +++ +  V        F  N+D+  ++   I G    V+   E      S+G +    F   SSD              K ++ G V  R    P     Y  LK  QI   + P+R +Q  ++     +KP +    N + +   ++  + AR +   ++D++ S FEKHQ+YN+KDL+D+T QP GY+KEILKE+   +     ++ WELKPEYRHY
Sbjct:   18 VWLVKVPKYLSQQWAKAPGRGEVGKLRIAKNQGRTEV-------SFTLNEDLANIHD--IGGKPASVSAPREHPFVLQSVGGQTLTVFTESSSD--------------KLSLEGIVVQRAECRPAASENYMKLKRLQIEESSKPVRLSQQADKVVTTNYKPVANHQYNIEYERKKKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVGYLKEILKEIGIQNVKGIHKNTWELKPEYRHY 242
[Close]
BLAST of General transcription factor IIF subunit 2 vs. UniProt/SwissProt
Match: sp|Q03123|T2FB_XENLA (General transcription factor IIF subunit 2 OS=Xenopus laevis OX=8355 GN=gtf2f2 PE=2 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 7.310e-33
Identity = 76/258 (29.46%), Postives = 134/258 (51.94%), Query Frame = 1 
Query:   85 VDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRY-KKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGVE-FKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            ++ ++ +  +WLVK+P YL+ +W  +     V K+ + +++ K  V        F  N+++ ++      G     ++ S  R     + S  G+T  + +  + G      + R   K A+ G V  R    P   + Y  +K KQI   + P R++Q  E+     +KP S    N + +   +D  + AR++  +++D++ S FEKHQ+YNIKDL+D+T QP  Y+KEIL+++   +   + ++ WELKPEYRHY
Sbjct:   10 LNGAKQNTGMWLVKLPKYLAQQWAKATGRGEVGKLRIVKNQGKTEV-------SFTLNEELASIQDI---GGKPASSLVSTPREHPFLLQSVGGQTLTVLTESLSGQSEDKSENRVIDKLALEGIVVHRAECRPAASDNYMQMKRKQIEESSKPKRQSQQLEKAVTSNYKPVSNHQYNIEYEKKKKDDGKRARVDKHQVLDMLFSAFEKHQYYNIKDLVDITKQPVTYLKEILRDIGIYNMKGTHKNTWELKPEYRHY 257
[Close]
BLAST of General transcription factor IIF subunit 2 vs. UniProt/SwissProt
Match: sp|P41900|T2FB_DROME (General transcription factor IIF subunit 2 OS=Drosophila melanogaster OX=7227 GN=TfIIFbeta PE=2 SV=2)

HSP 1 Score: 124.405 bits (311), Expect = 8.922e-32
Identity = 81/260 (31.15%), Postives = 128/260 (49.23%), Query Frame = 1 
Query:   85 VDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIK---NKDIPTVNKFIIQGITEK-VNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            +D S     VWLVK+P Y++ +W  +P N  V K+ +++   ++A   L   P  +     + IPT +   +  +T++ + V S        +   DGK         E       +P  +K  + GR+  +    P  DN Y  LK + IR  + P R+ Q  ++    FKP      N +     +   + AR + + +MD++   FEKHQ+YNIKDL+ +T QP  Y+KEILK+V   +     ++MWELK EYRHY
Sbjct:   16 LDLSNAGRGVWLVKVPKYIAQKWEKAPTNMDVGKLRINKTPGQKAQVSLSLTPAVLALDPEEKIPTEHILDVSQVTKQTLGVFSH-------MAPSDGK---------ENSTTSAAQPDNEKLYMEGRIVQKLECRPIADNCYMKLKLESIRKASEPQRRVQPIDKIVQNFKPVKDHAHNIEYRERKKAEGKKARDDKNAVMDMLFHAFEKHQYYNIKDLVKITNQPISYLKEILKDVCDYNMKNPHKNMWELKKEYRHY 259
[Close]
BLAST of General transcription factor IIF subunit 2 vs. UniProt/SwissProt
Match: sp|Q8R0A0|T2FB_MOUSE (General transcription factor IIF subunit 2 OS=Mus musculus OX=10090 GN=Gtf2f2 PE=1 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 1.126e-31
Identity = 78/248 (31.45%), Postives = 122/248 (49.19%), Query Frame = 1 
Query:  112 VWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQ-GVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            VWLVK+P YLS +W  +     V K+ + +++ +  V        F  N+D+  ++   I G    V+   E      S+G +    F   SSD              K ++ G V  R    P     Y  LK  QI   + P+R +Q  ++     +KP +    N + +   ++  + AR +   ++D++ S FEKHQ+YN+KDL+D+T QP GY+KEILKE+   +     ++ WELKPEYRHY
Sbjct:   18 VWLVKVPKYLSQQWAKASGRGEVGKLRIAKNQGRTEV-------SFTLNEDLANIHD--IGGKPASVSAPREHPFVLQSVGGQTLTVFTESSSD--------------KLSLEGIVVQRAECRPAASENYMKLKRLQIEESSKPVRLSQQLDKVVTTNYKPVANHQYNIEYERKKKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVGYLKEILKEIGIQNVKGIHKNTWELKPEYRHY 242
[Close]
BLAST of General transcription factor IIF subunit 2 vs. UniProt/SwissProt
Match: sp|Q2T9L9|T2FB_BOVIN (General transcription factor IIF subunit 2 OS=Bos taurus OX=9913 GN=GTF2F2 PE=2 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 1.522e-31
Identity = 77/248 (31.05%), Postives = 123/248 (49.60%), Query Frame = 1 
Query:  112 VWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQ-GVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            VWLVK+P YLS +W  +P    V K+ + +++ +  V        F  N+D+  ++   I G    V+   E      S+G +    F   SSD              K ++ G V  R    P  +  Y  LK  QI   + P+R +Q  ++     +KP +    N + +   ++  + AR +   ++D++ S FEKHQ+YN+KDL+D+T QP  Y+K+ILKE+   +     ++ WELKPEYRHY
Sbjct:   18 VWLVKVPKYLSQQWAKAPGRGEVGKLRIAKNQGRTEV-------SFTLNEDLANIHD--IGGKPASVSAPREHPFVLQSVGGQTLTVFTESSSD--------------KLSLEGIVVQRAECRPAANENYMRLKRLQIEESSKPVRLSQQLDKVVTTNYKPVANHQYNIEYERKKKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVSYLKDILKEIGVQNVKGIHKNTWELKPEYRHY 242
[Close]
BLAST of General transcription factor IIF subunit 2 vs. TrEMBL
Match: A0A0X3NM55 (General transcription factor IIF subunit 2 (Fragment) OS=Schistocephalus solidus OX=70667 GN=T2FB PE=4 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 7.128e-36
Identity = 84/282 (29.79%), Postives = 145/282 (51.42%), Query Frame = 1 
Query:   64 EDDAVYSVDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQ--------DKDKEAVYKLICNPDFI-----KNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSR-DGKTFILQSSD---IEGGYNPGKKPRYKKK-AIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHYS 855
            E   +Y+VD S+  D VWLVK+PNYL++ W+NS  +  V K+V+          D+  E+  +L+ +          +  +P  ++F+IQ  T  +   +  R  +    S   G+  ++   +   +  G +P    R  ++ ++ GRV+ R    PP ++ Y  LK++Q +  N    +  I +     ++P +    N + +   +   ++ R E   ++  +   FE+HQ+Y +KDL+ LT QP  Y+ E+LKE+AT +  P  +HMWELKPEYRHYS
Sbjct:   18 EAHPLYAVDTSRAKDGVWLVKVPNYLADLWLNSSSDGPVGKVVISTSSGVVGKGDQKPESQVRLVTDDALTIEAGEASSLVPKEHQFLIQ-TTPSLAAGNRGRPGDAKRSSAMSGQELVILCEEDVPVSSGSSPVNPSRVTRRYSLFGRVNSRAECRPPPNSKYMMLKAQQCKARNKARHEVCILDTPVRNYRPVANHEFNIEYEQRKKLEGKNVRRERDDVLQDLFKAFERHQYYAVKDLMLLTKQPMSYLTELLKEIATFNTKPPHKHMWELKPEYRHYS 298
[Close]
BLAST of General transcription factor IIF subunit 2 vs. TrEMBL
Match: A0A183T236 (General transcription factor IIF subunit 2 OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS10534 PE=4 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 3.100e-34
Identity = 85/288 (29.51%), Postives = 146/288 (50.69%), Query Frame = 1 
Query:   64 EDDAVYSVDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQ--------DKDKEAVYKLICNPDFI-----KNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSR-DGKTFILQSSD---IEGGYNPGKKPRYKKK-AIIGRVSVRCNVMPPDDNAYFALKSKQIRTY------NTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHYS 855
            E   +Y+VD S+  D VWLVK+PNYL++ W+NS  +  V K+V+          D+  E+  +L+ +          +  +P  ++F+IQ  T  +   +  R  +    S   G+  ++   +   +  G +P    R  ++ ++ GRV+ R    PP ++ Y  LK++Q+ T       N    +  I +     ++P +    N + +   +   ++ R E   ++  +   FE+HQ+Y +KDL+ LT QP  Y+ E+LKE+AT +  P  +HMWELKPEYRHYS
Sbjct:   10 EAHPLYAVDTSRAKDGVWLVKVPNYLADLWLNSSSDGPVGKVVISTSSGVVGKGDQKPESQVRLVTDDALTIEAGEASSLVPKEHQFLIQ-TTPSLAAGNRGRPGDAKRSSAMSGQELVILCEEDVPVSSGSSPVNPSRVTRRYSLFGRVNSRAECRPPPNSKYMMLKAQQVPTLPFCKARNKARHEVCILDTPVRNYRPVANHEFNIEYEQRKKLEGKNVRRERDDVLQDLFKAFERHQYYAVKDLMLLTKQPMSYLTELLKEIATFNTKPPHKHMWELKPEYRHYS 296
[Close]
BLAST of General transcription factor IIF subunit 2 vs. TrEMBL
Match: A0A0B1T9G2 (General transcription factor IIF subunit 2 OS=Oesophagostomum dentatum OX=61180 GN=OESDEN_06193 PE=3 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 3.816e-34
Identity = 78/259 (30.12%), Postives = 133/259 (51.35%), Query Frame = 1 
Query:   79 YSVDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHYS 855
            ++VD       VWLVK+P YLS+ W  +   + V ++V+ Q  + +   KL   P  +  + +    K   Q   E   +  +   + +++ + D K+ + +  D++ G          + ++ GRV  R    PP  N+Y  +K  QI     P ++    E+  V+FKP +    +  +    ++G ++ R + + LM+ +   FEKHQ+Y ++DL  LT +P GYVKEIL E+A  + AP  + MWELKPEYR+Y+
Sbjct:    7 HAVDCEMAKRGVWLVKVPRYLSDLW-EANAGTDVGRLVIQQGANGKGDVKLKSRPGLVLPQSVSATIKGPAQVPDEHSFILGDVLNQTMAVLAED-KSGLNEDVDVKSG----------RLSLEGRVVKRAECRPPASNSYLKMKIAQISNSGQPKKQVLQMEKAAVKFKPVAAHAEDMMRIKQKKEGAKTVRADRNVLMEALFHAFEKHQYYRLQDLQQLTQRPAGYVKEILTEIAVYNTAPPHKSMWELKPEYRNYA 253
[Close]
BLAST of General transcription factor IIF subunit 2 vs. TrEMBL
Match: A0A0R3U368 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS981 PE=4 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 8.320e-34
Identity = 86/290 (29.66%), Postives = 150/290 (51.72%), Query Frame = 1 
Query:   64 EDDAVYSVDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIV--------VDQDKDKEAVYKLICNPDFI-----KNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIG-SRDGKTFIL--------QSSDIEGGYNPGKK-PRY---KKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHYS 855
            E  A++ VD S+  D VWLVK+PNYL++ W+    + +V K+V        VD+D+ +++  +L+ +   I       K IP  ++F+IQ  +      ++ R  +     +  G+  ++         SS IE   +P +  PR    K+ ++ GRV+ R    PP + +Y  LK++Q++  N    +  + ++      P +   +N + +   +   ++ R E   ++  +   FEKHQ+Y  KDL+ LT QP  Y+ E+LKE+A  +  P  ++MWELKPEYRHY+
Sbjct:   12 EAHALHPVDTSRAKDGVWLVKVPNYLADLWLKPNTDRVVGKVVISTAASTSVDKDQKQKSDVRLVTDDSLIGEAGESGKLIPKEHQFLIQTASSASTAANKSRPGSSRGSSAMVGQELVILCEEDCQVPSSSIEALSSPTQGVPRSRAGKRYSLFGRVNSRAECQPPPNASYMKLKAQQLKAKNRARHEVCVLDKPVQTHLPVANHAVNLEYEARKKREGKNLRREREDVLRDLFKAFEKHQYYAFKDLLLLTKQPVTYLTELLKEIAVFNSRPPHQNMWELKPEYRHYA 301
[Close]
BLAST of General transcription factor IIF subunit 2 vs. TrEMBL
Match: A0A4S2M3Y5 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_002864 PE=4 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 2.151e-33
Identity = 88/303 (29.04%), Postives = 142/303 (46.86%), Query Frame = 1 
Query:   73 AVYSVDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQD----------KDKEAVYKLICNPDFIKNKD-----IPTVNKFIIQGITEKVNVKSEE--RAKNLSIGSRDGKTFILQSSD---IEGGYNPG---------------------KKPRYKKK-AIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHYS 855
            A+YSVD S+  D VWLVK+P YL+  W  S  + +V K+V+             +   +   L+ +PD + +       IP  ++F IQ      +  S    +A    + +  G+  I+ + D   + G    G                        RY ++ ++ GRV+ R    PP +  Y  LK++Q++  N P  + ++  E    +KP +    N + +   R   ++ R E   +M  +   FE+HQ+Y I+DLI LT QP  Y+ EILKE+A  +     ++MWELKPEYRHY+
Sbjct:   16 ALYSVDTSRAKDGVWLVKVPKYLAELWRKSGPDGVVGKVVIASSPAPGQAEGNRRPTGSQVTLVTDPDLLAHAGETGELIPKEHQFFIQTSASTSSAASSRPGQASQRRVSALSGQELIILAEDDLAVAGPSGTGTVCNSETDTSSARPSSPFSNSSSARYSRRLSLFGRVNSRAECRPPPNTRYMMLKAQQMKEINRPKHEVRLLYEPVRNYKPVANHVSNIEYEARKRAEGKNLRREKDDVMQDLFKAFERHQYYTIRDLILLTKQPVTYLTEILKEIAIFNPRAPHKNMWELKPEYRHYA 318
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Cavefish
Match: gtf2f2a (general transcription factor IIF subunit 2-like [Source:NCBI gene;Acc:103042992])

HSP 1 Score: 118.242 bits (295), Expect = 8.747e-31
Identity = 78/251 (31.08%), Postives = 124/251 (49.40%), Query Frame = 1 
Query:  112 VWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKT---FILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQ-GVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            VWLVK+P YLS +W  +     V K+ + +++ K  V        F  N+++ T++       +EK ++    R    ++ +  G+T   F   SSD              K A+ G V  R    P     Y  LK  QI     P R +Q  ++     +KP +    N   +   ++  + AR +  +++D++ S FEKHQFYNIKDL+D+T QP  Y+KEIL+++   +   + ++ WELKPEYRHY
Sbjct:   18 VWLVKVPKYLSQQWAKASGRGEVGKLRIGKNQGKAEV-------SFTLNEELTTID-----STSEKASLVRAPREHPFTMQTVGGQTLAVFTENSSD--------------KIALEGVVVQRAECRPAASENYMKLKKLQIEESLKPHRFSQQLDKAVTTNYKPVANHAYNLDYEKKKKEEGKRARADKQQVLDMLFSAFEKHQFYNIKDLVDITKQPVIYLKEILRDIGIYNVKGTHKNTWELKPEYRHY 242
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Cavefish
Match: gtf2f2a (general transcription factor IIF subunit 2-like [Source:NCBI gene;Acc:103042992])

HSP 1 Score: 117.857 bits (294), Expect = 2.119e-30
Identity = 75/253 (29.64%), Postives = 125/253 (49.41%), Query Frame = 1 
Query:  112 VWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYK-----KKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQ-GVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            VWLVK+P YLS +W  +     V K+ + +++ K  V        F  N+++ T++       +EK ++    R    ++ +  G+T  + + +  G  +             K A+ G V  R    P     Y  LK  QI     P R +Q  ++     +KP +    N   +   ++  + AR +  +++D++ S FEKHQFYNIKDL+D+T QP  Y+KEIL+++   +   + ++ WELKPEYRHY
Sbjct:   18 VWLVKVPKYLSQQWAKASGRGEVGKLRIGKNQGKAEV-------SFTLNEELTTID-----STSEKASLVRAPREHPFTMQTVGGQTLAVFTENSSGQSDAEGSSSGTGSGPDKIALEGVVVQRAECRPAASENYMKLKKLQIEESLKPHRFSQQLDKAVTTNYKPVANHAYNLDYEKKKKEEGKRARADKQQVLDMLFSAFEKHQFYNIKDLVDITKQPVIYLKEILRDIGIYNVKGTHKNTWELKPEYRHY 258
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Cavefish
Match: gtf2f2b (general transcription factor IIF subunit 2-like [Source:NCBI gene;Acc:103030467])

HSP 1 Score: 116.316 bits (290), Expect = 6.056e-30
Identity = 75/249 (30.12%), Postives = 125/249 (50.20%), Query Frame = 1 
Query:  112 VWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGV--EFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            VWLVK+P YLS +W  +     V K+ + +++ K  V        F  N+++      +++   EK +     R    ++ S  G+   + +   E   N        + A+ G V  R    P    +Y  +K  QI     P+R +Q C E+ V   +KP S  + N + +   ++  + AR +  K+++++ S FEKHQ+YNIK+L+++T QP  Y+KEIL+E+   +     +  WELKPEYRHY
Sbjct:   19 VWLVKVPKYLSQQWGKASGRGEVGKLKIAKNQGKTVV-------SFNLNEELT-----LVESSGEKASSVRAPREHPFTMQSVGGQVLAVFT---ESSSN--------QIAVEGMVVQRAECRPAVQESYVKVKKLQIEESTKPVRLSQ-CLEKAVTTNYKPVSNHSYNLEYEKKKKEQGKRARADREKVLEMLFSAFEKHQYYNIKNLVEITKQPVIYLKEILREIGVYNPKGPHKSTWELKPEYRHY 243
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Cavefish
Match: gtf2f2a (general transcription factor IIF subunit 2-like [Source:NCBI gene;Acc:103042992])

HSP 1 Score: 109.768 bits (273), Expect = 1.108e-27
Identity = 72/248 (29.03%), Postives = 117/248 (47.18%), Query Frame = 1 
Query:  112 VWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQ-GVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            VWLVK+P YLS +W  +     V+                     F  N+++ T++       +EK ++    R    ++ +  G+T  + + +  G           K A+ G V  R    P     Y  LK  QI     P R +Q  ++     +KP +    N   +   ++  + AR +  +++D++ S FEKHQFYNIKDL+D+T QP  Y+KEIL+++   +   + ++ WELKPEYRHY
Sbjct:   18 VWLVKVPKYLSQQWAKASGRGEVS---------------------FTLNEELTTID-----STSEKASLVRAPREHPFTMQTVGGQTLAVFTENSSGE---------NKIALEGVVVQRAECRPAASENYMKLKKLQIEESLKPHRFSQQLDKAVTTNYKPVANHAYNLDYEKKKKEEGKRARADKQQVLDMLFSAFEKHQFYNIKDLVDITKQPVIYLKEILRDIGIYNVKGTHKNTWELKPEYRHY 230
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Sea Lamprey
Match: gtf2f2b (general transcription factor IIF, polypeptide 2b [Source:ZFIN;Acc:ZDB-GENE-050417-374])

HSP 1 Score: 45.4394 bits (106), Expect = 5.032e-6
Identity = 35/163 (21.47%), Postives = 69/163 (42.33%), Query Frame = 1 
Query:   85 VDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPR--YKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICE 567
            +  ++ +  +WLVK+P YLS +W  + +   V K+ + + + K  V        F+ ++++       +QG + K    +  R   L++    G++  + +    G           Y K  + G V  R +  P    +Y  +K  QI   + P+R +Q  E
Sbjct:   10 LTGAKLNRGLWLVKVPKYLSQQWGKATERGEVGKLKISKGQGKTEV-------SFVLSEELAGGGG--VQGTSAKSTAINTPRDHVLALQGVAGQSLGVFTEGSSGKQTDATSGNVVYDKLFLEGVVVQRADCRPVVGESYMKMKRMQIEESSKPMRLSQQLE 163
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Yeast
Match: TFG2 (TFIIF (Transcription Factor II) middle subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP30 [Source:SGD;Acc:S000003237])

HSP 1 Score: 62.3882 bits (150), Expect = 2.986e-11
Identity = 73/301 (24.25%), Postives = 130/301 (43.19%), Query Frame = 1 
Query:   85 VDASQCDDRVWLVKIPNYLSNEWM--NSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQG---ITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIE------------------------------GGYNPGKKPRYKKKAIIGRVSVRCNVMPP-DDNAYFAL--KSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPS-----GRD----GTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYR 846
            +D  + + +VWLV++P +L+ +W   N+     + KI +++D  K  +     + D I ++    + K +++     TE+   K ++R K L       +   L+  + E                                Y P  K   KK AI+G V   C VMP  +D  Y  +  + + I   N   R T + E  GV     S   ++ + D S     GR+      +S RM   +++D +   F+++ ++++K L + T QP  ++KE L +VATL K       + L+PEY+
Sbjct:   59 LDLERSNRQVWLVRLPMFLAEKWRDRNNLHGQELGKIRINKDGSKITLLLNENDNDSIPHEYDLELTKKVVENEYVFTEQNLKKYQQRKKELEADPEKQRQAYLKKQEREEELKKKQQQQKRRNNRKKFNHRVMTDRDGRDRYIPYVKTIPKKTAIVGTVCHECQVMPSMNDPNYHKIVEQRRNIVKLNNKERITTLDETVGVTM---SHTGMSMRSDNSNFLKVGREKAKSNIKSIRMPKKEILDYLFKLFDEYDYWSLKGLKERTRQPEAHLKECLDKVATLVKKGPYAFKYTLRPEYK 356
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Nematostella
Match: EDO36378 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SIX2])

HSP 1 Score: 107.071 bits (266), Expect = 5.128e-27
Identity = 85/261 (32.57%), Postives = 123/261 (47.13%), Query Frame = 1 
Query:   85 VDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVA---KIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPD-DNAYFALKSKQIRTYNTPLRKTQICEEQGVE-FKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            +D +     VWLVK+P YLSN WM   D S +    KI  DQ   KE +        F   +++  + K I+ G          ++   LSI    G      S+     Y   +K      A+ GRV  R +  P +  N YF  K K I+  + P R+ Q  ++     +KP S   +N +     +D  + AR     +   + S FE+HQ+YN+KDL+  T QP  Y+K ILKE+A  +     ++MWELKPEYRHY
Sbjct:    5 LDCNGAGRDVWLVKVPKYLSNLWMEKADPSGIVGTLKINKDQVGKKEKIVFF-----FASFRELEILKKKILPG----------DKECGLSIALFYG------SNKFFNYYYTSEKL-----AVEGRVVQRADCRPLNPSNDYFLHKRKSIQVASKPARQAQQLKKIVTNAYKPVSDHKMNIEYKKKKKDEGKRARATEEDVKASLFSAFEQHQYYNLKDLVHKTQQPIVYLKSILKEIAQYNTKNPHKNMWELKPEYRHY 239
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Medaka
Match: gtf2f2a (general transcription factor IIF subunit 2 [Source:NCBI gene;Acc:101157760])

HSP 1 Score: 113.235 bits (282), Expect = 5.215e-29
Identity = 76/257 (29.57%), Postives = 123/257 (47.86%), Query Frame = 1 
Query:   85 VDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEK-VNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            +  ++ +  VWLVK+P YL+ +W  +     V K+            ++I    F  N+++      II+GI +K V+   +      S+G +    F   SSD              K A+ G V  R    P    +Y  LK  QI   + P R +Q   +    +KP      N + +       + AR +  ++++++ S FEKHQ+YNIKDL+D+T QP  Y+KEIL+++   +   + ++ WELKPEYRHY
Sbjct:    9 LTGAKLNTGVWLVKVPKYLAQQWAKATGRGEVGKL------------RIIKYVSFTLNEELT-----IIEGIEDKTVSAPRDHPFTMQSVGGQMLAVFTESSSD--------------KIALEGVVVQRAECRPAVSESYMKLKRLQIEESSKPSRLSQQLSKPVTNYKP----VANHEYNQHVHKICKRARADKQQVLEMLFSAFEKHQYYNIKDLVDITKQPVIYLKEILRDIGIYNVKGTHKNTWELKPEYRHY 230
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Medaka
Match: gtf2f2a (general transcription factor IIF subunit 2 [Source:NCBI gene;Acc:101157760])

HSP 1 Score: 111.309 bits (277), Expect = 6.336e-28
Identity = 77/265 (29.06%), Postives = 133/265 (50.19%), Query Frame = 1 
Query:   85 VDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPD--FIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFIL-------QSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            +  ++ +  VWLVK+P YL+ +W  +     V K+ + +  +       +  P+  F  N+++      II+GI +K    S  R    ++ S  G+   +       QS +   G + G      K A+ G V  R    P    +Y  LK  QI   + P R +Q   +    +KP +    N++ +   ++  + AR +  ++++++ S FEKHQ+YNIKDL+D+T QP  Y+KEIL+++   +   + ++ WELKPEYRHY
Sbjct:    9 LTGAKLNTGVWLVKVPKYLAQQWAKATGRGEVGKLRIIKKGN-------LGKPEVSFTLNEELT-----IIEGIEDKT--VSAPRDHPFTMQSVGGQMLAVFTESSSGQSEERSDGSSSGAGSGPDKIALEGVVVQRAECRPAVSESYMKLKRLQIEESSKPSRLSQQLSKPVTNYKPVANHEYNREYEKKKKEEGKRARADKQQVLEMLFSAFEKHQYYNIKDLVDITKQPVIYLKEILRDIGIYNVKGTHKNTWELKPEYRHY 259
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Medaka
Match: gtf2f2b (general transcription factor IIF subunit 2 [Source:NCBI gene;Acc:101164354])

HSP 1 Score: 101.293 bits (251), Expect = 1.555e-24
Identity = 67/249 (26.91%), Postives = 107/249 (42.97%), Query Frame = 1 
Query:  112 VWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAII--GRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            VWLVK+P YLS +W  + +   V KI                           T+ K++   ++E++        K+ S+       F +Q+    GG       +     I   G V  R    P     Y  +K  Q  +        Q+       FKP +    N + +   +   +  R E   +MD++ S FEKHQ+Y+ KDL+D+T QP  Y+KEI++E+   ++  + +  WELKPEY+HY
Sbjct:   18 VWLVKVPKYLSQQWDKATEKGEVGKI---------------------------TIGKYVCFSLSEELAALGSAEEKDASVQVPRDHPFTMQTV---GGQTMAVFSQSDTDGISLEGTVVHRAECRPNVSEDYMKIKRLQSESVKPQRLSQQLDRAITTVFKPVANHDFNVEFEKKKKSEGKMVRAERQVVMDMLFSAFEKHQYYSFKDLVDITKQPVTYLKEIVREIGVCNRKGAHKSSWELKPEYQHY 236
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Medaka
Match: gtf2f2b (general transcription factor IIF subunit 2 [Source:NCBI gene;Acc:101164354])

HSP 1 Score: 81.6481 bits (200), Expect = 4.283e-17
Identity = 36/90 (40.00%), Postives = 57/90 (63.33%), Query Frame = 1 
Query:  583 FKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            FKP +    N + +   +   +  R E   +MD++ S FEKHQ+Y+ KDL+D+T QP  Y+KEI++E+   ++  + +  WELKPEY+HY
Sbjct:  204 FKPVANHDFNVEFEKKKKSEGKMVRAERQVVMDMLFSAFEKHQYYSFKDLVDITKQPVTYLKEIVREIGVCNRKGAHKSSWELKPEYQHY 293
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Planmine SMEST
Match: SMESG000026639.1 (SMESG000026639.1)

HSP 1 Score: 556.599 bits (1433), Expect = 0.000e+0
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 1 
Query:   52 MIDPEDDAVYSVDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHYS 855
            MIDPEDDAVYSVDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHYS
Sbjct:    1 MIDPEDDAVYSVDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHYS 268
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Planmine SMEST
Match: SMESG000026639.1 (SMESG000026639.1)

HSP 1 Score: 503.827 bits (1296), Expect = 0.000e+0
Identity = 248/268 (92.54%), Postives = 248/268 (92.54%), Query Frame = 1 
Query:   52 MIDPEDDAVYSVDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHYS 855
            MIDPEDDAVYSVDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLIC                    ITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHYS
Sbjct:    1 MIDPEDDAVYSVDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLIC--------------------ITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHYS 248
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Planmine SMEST
Match: SMESG000070633.1 (SMESG000070633.1)

HSP 1 Score: 226.098 bits (575), Expect = 3.663e-72
Identity = 115/272 (42.28%), Postives = 174/272 (63.97%), Query Frame = 1 
Query:   52 MIDPEDDAVYSVDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRS-----ARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            M    DD VY+V++ + +  +WLVK+PNY+ ++W+NSP++SIVAKIV+D +      YK ICNPD +KN++  T NK +IQ   +    KSE +  +LS+G+R+ K+F+L+++         +K    KKA+IG+V+V+ NVMP ++  YF+ KSKQ++ +N P R ++ C+ +    K          K P+ R   +S      R++  K+M+LICS FE HQ+Y+IKDL+ LT Q   YVKEIL E+A +S  PS+R+ WELK E+RHY
Sbjct:    1 MNSENDDQVYAVNSERANQNMWLVKVPNYIYDDWINSPEDSIVAKIVIDSEDQSNKSYKFICNPDCLKNQEKATTNKLVIQNSYD--FKKSENKKVDLSVGTRNNKSFVLKTT---------RKNIKTKKALIGQVAVQANVMPDNNEGYFSWKSKQVKIFNEPARTSKFCDIKSQMIKKSEIP-----KKPNNRKILKSFANSKIRIDRRKMMELICSCFETHQYYSIKDLVSLTKQSEAYVKEILLEIAKISTLPSQRNKWELKEEFRHY 256
[Close]
BLAST of General transcription factor IIF subunit 2 vs. Planmine SMEST
Match: SMESG000070634.1 (SMESG000070634.1)

HSP 1 Score: 225.713 bits (574), Expect = 5.120e-72
Identity = 110/267 (41.20%), Postives = 171/267 (64.04%), Query Frame = 1 
Query:   52 MIDPEDDAVYSVDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQDKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSIGSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAYFALKSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRSARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSKAPSRRHMWELKPEYRHY 852
            M    DD VY+V++ + + ++WLVK+PNY+ ++W+NSP++SIVAKIV+D +      YK ICNPD +KN++  T NK +IQ   +    KSE +  +LS+G+R+ K+F+L+++         +K    KK++IG+V+V+ NVMP ++  YF+ KSKQ++ +N P R ++ C+ +    K      +   +           R++  K+M+LICS FE HQ+Y+IKDL+ LT Q   YVKEIL E+A +S  PS+R+ WELK E+RHY
Sbjct:    1 MNSENDDQVYAVNSERANQKMWLVKVPNYIYDDWINSPEDSIVAKIVIDSEDQSNKSYKFICNPDCLKNQEKATTNKLVIQNSYD--FKKSENKKVDLSVGTRNNKSFVLKTT---------RKNLKTKKSLIGQVAVQANVMPDNNEGYFSWKSKQVKIFNEPARTSKFCDIKSQMIKKSEIPKMPNNRKILKSFANSKIRIDRRKMMELICSCFETHQYYSIKDLVSLTKQSEAYVKEILLEIAKISTLPSQRNKWELKEEFRHY 256
The following BLAST results are available for this feature:
BLAST of General transcription factor IIF subunit 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
GTF2F21.084e-3031.05general transcription factor IIF subunit 2 [Source... [more]
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BLAST of General transcription factor IIF subunit 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gtf-2F24.537e-2328.04General transcription factor IIF subunit 2 [Sourc... [more]
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BLAST of General transcription factor IIF subunit 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
TfIIFbeta8.900e-3331.15gene:FBgn0010421 transcript:FBtr0082310[more]
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BLAST of General transcription factor IIF subunit 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
gtf2f2a2.062e-3532.16general transcription factor IIF, polypeptide 2a [... [more]
gtf2f2a1.716e-3432.27general transcription factor IIF, polypeptide 2a [... [more]
gtf2f2a1.716e-3432.27general transcription factor IIF, polypeptide 2a [... [more]
gtf2f2b1.178e-3231.46general transcription factor IIF, polypeptide 2b [... [more]
gtf2f2a8.635e-2637.86general transcription factor IIF, polypeptide 2a [... [more]
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BLAST of General transcription factor IIF subunit 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
gtf2f26.829e-3329.07mitogen-activated protein kinase 12 [Source:Xenbas... [more]
gtf2f23.571e-3228.79mitogen-activated protein kinase 12 [Source:Xenbas... [more]
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BLAST of General transcription factor IIF subunit 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Gtf2f21.610e-3231.45general transcription factor IIF, polypeptide 2 [S... [more]
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BLAST of General transcription factor IIF subunit 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q01750|T2FB_RAT5.292e-3331.85General transcription factor IIF subunit 2 OS=Ratt... [more]
sp|Q03123|T2FB_XENLA7.310e-3329.46General transcription factor IIF subunit 2 OS=Xeno... [more]
sp|P41900|T2FB_DROME8.922e-3231.15General transcription factor IIF subunit 2 OS=Dros... [more]
sp|Q8R0A0|T2FB_MOUSE1.126e-3131.45General transcription factor IIF subunit 2 OS=Mus ... [more]
sp|Q2T9L9|T2FB_BOVIN1.522e-3131.05General transcription factor IIF subunit 2 OS=Bos ... [more]
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BLAST of General transcription factor IIF subunit 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0X3NM557.128e-3629.79General transcription factor IIF subunit 2 (Fragme... [more]
A0A183T2363.100e-3429.51General transcription factor IIF subunit 2 OS=Schi... [more]
A0A0B1T9G23.816e-3430.12General transcription factor IIF subunit 2 OS=Oeso... [more]
A0A0R3U3688.320e-3429.66Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A4S2M3Y52.151e-3329.04Uncharacterized protein OS=Opisthorchis felineus O... [more]
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BLAST of General transcription factor IIF subunit 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
gtf2f2a8.747e-3131.08general transcription factor IIF subunit 2-like [S... [more]
gtf2f2a2.119e-3029.64general transcription factor IIF subunit 2-like [S... [more]
gtf2f2b6.056e-3030.12general transcription factor IIF subunit 2-like [S... [more]
gtf2f2a1.108e-2729.03general transcription factor IIF subunit 2-like [S... [more]
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BLAST of General transcription factor IIF subunit 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gtf2f2b5.032e-621.47general transcription factor IIF, polypeptide 2b [... [more]
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BLAST of General transcription factor IIF subunit 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
TFG22.986e-1124.25TFIIF (Transcription Factor II) middle subunit; in... [more]
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BLAST of General transcription factor IIF subunit 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO363785.128e-2732.57Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of General transcription factor IIF subunit 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 4
Match NameE-valueIdentityDescription
gtf2f2a5.215e-2929.57general transcription factor IIF subunit 2 [Source... [more]
gtf2f2a6.336e-2829.06general transcription factor IIF subunit 2 [Source... [more]
gtf2f2b1.555e-2426.91general transcription factor IIF subunit 2 [Source... [more]
gtf2f2b4.283e-1740.00general transcription factor IIF subunit 2 [Source... [more]
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BLAST of General transcription factor IIF subunit 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000026639.10.000e+0100.00SMESG000026639.1[more]
SMESG000026639.10.000e+092.54SMESG000026639.1[more]
SMESG000070633.13.663e-7242.28SMESG000070633.1[more]
SMESG000070634.15.120e-7241.20SMESG000070634.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027693 ID=SMED30027693|Name=General transcription factor IIF subunit 2|organism=Schmidtea mediterranea sexual|type=transcript|length=1122bp
TTTTTTTTTGTTCATTTAAAAGATAATTTAATTTTATAAAAATATTTTAG
CATGATTGATCCTGAAGATGATGCAGTTTATTCAGTAGATGCATCACAAT
GTGACGATAGGGTTTGGTTGGTAAAAATTCCCAATTATTTATCAAATGAA
TGGATGAACTCTCCGGACAATTCTATTGTTGCAAAAATTGTAGTGGATCA
AGATAAAGATAAAGAAGCTGTCTACAAATTAATTTGTAATCCCGACTTTA
TAAAAAATAAAGACATACCAACAGTAAACAAATTTATTATTCAAGGTATT
ACTGAAAAAGTTAATGTTAAATCCGAAGAACGAGCTAAAAACTTATCTAT
TGGTTCCCGTGATGGTAAAACGTTCATATTGCAATCATCTGATATCGAAG
GAGGCTATAACCCAGGAAAAAAGCCAAGATATAAGAAAAAAGCCATTATC
GGCCGTGTAAGTGTAAGATGTAATGTCATGCCACCTGATGACAATGCATA
TTTCGCATTGAAATCTAAACAAATTAGAACTTATAACACCCCGTTAAGAA
AAACTCAAATTTGTGAGGAACAAGGTGTGGAGTTTAAACCGAAATCCACC
GGCACAATCAATAAAAAGAAAGATCCCAGTGGTCGAGATGGAACTAGATC
AGCCCGAATGGAGCACAGCAAACTCATGGACTTGATCTGTTCACATTTTG
AGAAACATCAATTTTATAATATCAAAGACTTGATCGATCTAACTGGTCAA
CCACCGGGATATGTGAAGGAAATATTGAAAGAAGTTGCCACATTAAGCAA
AGCTCCTTCCCGTCGCCACATGTGGGAACTGAAACCCGAATATCGGCATT
ATTCCTAATGTTTTATAAAAATTTTAAATATTGCCTTGTTTTTTGTTGGA
TTAATTGATTAAATTTTGCTAGCTGAATTGATTTATACATTGAATGATGA
TTACTGATTGCTTTCTTCATTTGTTGCATCAGTTATTTTTCATTGGAGTA
TTTTCTATTGGTCGAGATGATATTTTGATGTGTTTAAATGTTATTGTTAC
ATTTCCCAAATCAATGGCGAATTTATTCTCCAATCCGGATTTTTAAATAC
ATTGTAAATTCGGCAGATCGGA
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protein sequence of SMED30027693-orf-1

>SMED30027693-orf-1 ID=SMED30027693-orf-1|Name=SMED30027693-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=269bp
MIDPEDDAVYSVDASQCDDRVWLVKIPNYLSNEWMNSPDNSIVAKIVVDQ
DKDKEAVYKLICNPDFIKNKDIPTVNKFIIQGITEKVNVKSEERAKNLSI
GSRDGKTFILQSSDIEGGYNPGKKPRYKKKAIIGRVSVRCNVMPPDDNAY
FALKSKQIRTYNTPLRKTQICEEQGVEFKPKSTGTINKKKDPSGRDGTRS
ARMEHSKLMDLICSHFEKHQFYNIKDLIDLTGQPPGYVKEILKEVATLSK
APSRRHMWELKPEYRHYS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
Vocabulary: INTERPRO
TermDefinition
IPR040504TFIIF_beta_N
IPR040450TFIIF_beta_HTH
IPR036388WH-like_DNA-bd_sf
IPR036390WH_DNA-bd_sf
IPR003196TFIIF_beta
IPR011991WHTH_DNA-bd_dom
IPR011039TFIIF_interaction
Vocabulary: cellular component
TermDefinition
GO:0005674transcription factor TFIIF complex
Vocabulary: biological process
TermDefinition
GO:0006367transcription initiation from RNA polymerase II promoter
GO:0006366transcription from RNA polymerase II promoter
Vocabulary: molecular function
TermDefinition
GO:0003824catalytic activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR040504TFIIF, beta subunit, N-terminalPFAMPF17683TFIIF_beta_Ncoord: 19..145
e-value: 5.4E-12
score: 46.6
IPR040450TFIIF beta subunit, HTH domainPFAMPF02270TFIIF_betacoord: 201..265
e-value: 4.5E-20
score: 71.5
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3DG3DSA:1.10.10.10coord: 200..268
e-value: 2.6E-24
score: 87.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 183..201
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 179..201
NoneNo IPR availablePANTHERPTHR10445:SF0GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2coord: 10..267
IPR003196Transcription initiation factor IIF, beta subunitPANTHERPTHR10445GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2coord: 10..267
IPR011039Transcription Factor IIF, Rap30/Rap74, interactionSUPERFAMILYSSF50916Rap30/74 interaction domainscoord: 11..89
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILYSSF46785"Winged helix" DNA-binding domaincoord: 201..267