Kunitz-type protease inhibitor

Overview


Name	Kunitz-type protease inhibitor
Smed ID	SMED30027656
Length (bp)	215

Neoblast Clusters

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30027656

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 2

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication	Specimen	Lifecycle	Evidence
prepharyngeal region	SMED30027656		SmedASXL_079247	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
parenchyma	SMED30027656		dd_Smed_v4_6318_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of Kunitz-type protease inhibitor vs. Ensembl Human
Match: APLP2 (amyloid beta precursor like protein 2 [Source:HGNC Symbol;Acc:HGNC:598])

HSP 1 Score: 57.7658 bits (138), Expect = 4.343e-11
Identity = 23/52 (44.23%), Postives = 34/52 (65.38%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            C+    TG C A   R+ FD   G+C++FIYGGC  N+NNF++++ C  +CK
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCK 361

[Close]

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            C+    TG C A   R+ FD   G+C++FIYGGC  N+NNF++++ C  +CK
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCK 361

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Human
Match: APLP2 (amyloid beta precursor like protein 2 [Source:HGNC Symbol;Acc:HGNC:598])

HSP 1 Score: 57.7658 bits (138), Expect = 4.787e-11
Identity = 23/52 (44.23%), Postives = 34/52 (65.38%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            C+    TG C A   R+ FD   G+C++FIYGGC  N+NNF++++ C  +CK
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCK 361

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Human
Match: APLP2 (amyloid beta precursor like protein 2 [Source:HGNC Symbol;Acc:HGNC:598])

HSP 1 Score: 57.3806 bits (137), Expect = 5.178e-11
Identity = 23/52 (44.23%), Postives = 34/52 (65.38%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            C+    TG C A   R+ FD   G+C++FIYGGC  N+NNF++++ C  +CK
Sbjct:  320 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCK 371

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BLAST of Kunitz-type protease inhibitor vs. Ensembl Human
Match: EPPIN (epididymal peptidase inhibitor [Source:HGNC Symbol;Acc:HGNC:15932])

HSP 1 Score: 54.299 bits (129), Expect = 8.504e-11
Identity = 26/53 (49.06%), Postives = 32/53 (60.38%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN 214
            C    ETG C AYF+ + +D K   C  F+YGGC  N NNF++K  C N CKN
Sbjct:   61 CEMPKETGPCLAYFLHWWYDKKDNTCSMFVYGGCQGNNNNFQSKANCLNTCKN 113

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BLAST of Kunitz-type protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667211:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3d.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 60.077 bits (144), Expect = 2.618e-12
Identity = 27/64 (42.19%), Postives = 38/64 (59.38%), Query Frame = 2

Query:   17 IVTFNFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            +V    S +RA  C+   E G CD Y +R+ F+  L EC  F +GGC  N+NNF+  E+CE+ C
Sbjct:  526 VVGLQTSGARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 589

HSP 2 Score: 57.3806 bits (137), Expect = 2.059e-11
Identity = 26/59 (44.07%), Postives = 30/59 (50.85%), Query Frame = 2

Query:   38 ESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN 214
            +S    C   IE G C   F R+ FD  +  C  F YGGC  N NNF T +EC N C N
Sbjct:  851 KSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKCVN 909

HSP 3 Score: 47.3654 bits (111), Expect = 8.361e-8
Identity = 20/51 (39.22%), Postives = 29/51 (56.86%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            C    + G+C  Y  R+ F+++ G+C QF YG C  N+NNF  +  CE  C
Sbjct: 2168 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 2218

HSP 4 Score: 46.9802 bits (110), Expect = 9.685e-8
Identity = 21/51 (41.18%), Postives = 28/51 (54.90%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            C    ++G C   F+R+ FD +   C  F Y GC  N NNF +KEEC  +C
Sbjct:  728 CLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAIC 778

HSP 5 Score: 46.9802 bits (110), Expect = 1.111e-7
Identity = 22/60 (36.67%), Postives = 32/60 (53.33%), Query Frame = 2

Query:   29 NFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            N +     +C   +E G C  +  R+ F+   G C  F YGGC  N+N+F T++ECE  C
Sbjct: 1065 NLAAQIEEKCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 1124

HSP 6 Score: 45.4394 bits (106), Expect = 3.783e-7
Identity = 20/52 (38.46%), Postives = 28/52 (53.85%), Query Frame = 2

Query:   53 ECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            EC      G C   F RY ++    +C QF Y GC  N NN++++E CE+ C
Sbjct:  422 ECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 473

HSP 7 Score: 44.2838 bits (103), Expect = 9.056e-7
Identity = 20/53 (37.74%), Postives = 26/53 (49.06%), Query Frame = 2

Query:   50 PECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            P C   +  G C    +RY +D     C  F Y GC  N NNF + E+C+ LC
Sbjct: 2731 PLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLC 2783

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667211:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 60.077 bits (144), Expect = 2.618e-12
Identity = 27/64 (42.19%), Postives = 38/64 (59.38%), Query Frame = 2

Query:   17 IVTFNFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            +V    S +RA  C+   E G CD Y +R+ F+  L EC  F +GGC  N+NNF+  E+CE+ C
Sbjct:  526 VVGLQTSGARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 589

HSP 2 Score: 57.3806 bits (137), Expect = 2.059e-11
Identity = 26/59 (44.07%), Postives = 30/59 (50.85%), Query Frame = 2

Query:   38 ESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN 214
            +S    C   IE G C   F R+ FD  +  C  F YGGC  N NNF T +EC N C N
Sbjct:  860 KSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKCVN 918

HSP 3 Score: 47.3654 bits (111), Expect = 8.361e-8
Identity = 20/51 (39.22%), Postives = 29/51 (56.86%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            C    + G+C  Y  R+ F+++ G+C QF YG C  N+NNF  +  CE  C
Sbjct: 2177 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 2227

HSP 4 Score: 46.9802 bits (110), Expect = 9.685e-8
Identity = 21/51 (41.18%), Postives = 28/51 (54.90%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            C    ++G C   F+R+ FD +   C  F Y GC  N NNF +KEEC  +C
Sbjct:  737 CLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAIC 787

HSP 5 Score: 46.9802 bits (110), Expect = 1.111e-7
Identity = 22/60 (36.67%), Postives = 32/60 (53.33%), Query Frame = 2

Query:   29 NFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            N +     +C   +E G C  +  R+ F+   G C  F YGGC  N+N+F T++ECE  C
Sbjct: 1074 NLAAQIEEKCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 1133

HSP 6 Score: 45.4394 bits (106), Expect = 3.783e-7
Identity = 20/52 (38.46%), Postives = 28/52 (53.85%), Query Frame = 2

Query:   53 ECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            EC      G C   F RY ++    +C QF Y GC  N NN++++E CE+ C
Sbjct:  422 ECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 473

HSP 7 Score: 44.2838 bits (103), Expect = 9.056e-7
Identity = 20/53 (37.74%), Postives = 26/53 (49.06%), Query Frame = 2

Query:   50 PECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            P C   +  G C    +RY +D     C  F Y GC  N NNF + E+C+ LC
Sbjct: 2740 PLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLC 2792

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667209:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3c.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 59.6918 bits (143), Expect = 3.277e-12
Identity = 27/64 (42.19%), Postives = 38/64 (59.38%), Query Frame = 2

Query:   17 IVTFNFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            +V    S +RA  C+   E G CD Y +R+ F+  L EC  F +GGC  N+NNF+  E+CE+ C
Sbjct:  397 VVGLQTSGARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 460

HSP 2 Score: 57.3806 bits (137), Expect = 2.654e-11
Identity = 26/59 (44.07%), Postives = 30/59 (50.85%), Query Frame = 2

Query:   38 ESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN 214
            +S    C   IE G C   F R+ FD  +  C  F YGGC  N NNF T +EC N C N
Sbjct:  731 KSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKCVN 789

HSP 3 Score: 46.9802 bits (110), Expect = 9.777e-8
Identity = 20/51 (39.22%), Postives = 29/51 (56.86%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            C    + G+C  Y  R+ F+++ G+C QF YG C  N+NNF  +  CE  C
Sbjct: 2048 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 2098

HSP 4 Score: 46.9802 bits (110), Expect = 1.189e-7
Identity = 20/46 (43.48%), Postives = 27/46 (58.70%), Query Frame = 2

Query:   71 ETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            ++G C   F+R+ FD +   C  F Y GC  N NNF +KEEC  +C
Sbjct:  613 DSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAIC 658

HSP 5 Score: 46.595 bits (109), Expect = 1.325e-7
Identity = 22/60 (36.67%), Postives = 32/60 (53.33%), Query Frame = 2

Query:   29 NFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            N +     +C   +E G C  +  R+ F+   G C  F YGGC  N+N+F T++ECE  C
Sbjct:  945 NLAAQIEEKCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 1004

HSP 6 Score: 45.0542 bits (105), Expect = 4.512e-7
Identity = 20/52 (38.46%), Postives = 28/52 (53.85%), Query Frame = 2

Query:   53 ECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            EC      G C   F RY ++    +C QF Y GC  N NN++++E CE+ C
Sbjct:  293 ECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 344

HSP 7 Score: 44.2838 bits (103), Expect = 1.091e-6
Identity = 20/53 (37.74%), Postives = 26/53 (49.06%), Query Frame = 2

Query:   50 PECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            P C   +  G C    +RY +D     C  F Y GC  N NNF + E+C+ LC
Sbjct: 2611 PLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLC 2663

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667209:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3h.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 59.3066 bits (142), Expect = 5.547e-12
Identity = 27/64 (42.19%), Postives = 38/64 (59.38%), Query Frame = 2

Query:   17 IVTFNFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            +V    S +RA  C+   E G CD Y +R+ F+  L EC  F +GGC  N+NNF+  E+CE+ C
Sbjct:  305 VVGLQTSGARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 368

HSP 2 Score: 56.6102 bits (135), Expect = 4.363e-11
Identity = 26/59 (44.07%), Postives = 30/59 (50.85%), Query Frame = 2

Query:   38 ESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN 214
            +S    C   IE G C   F R+ FD  +  C  F YGGC  N NNF T +EC N C N
Sbjct:  639 KSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKCVN 697

HSP 3 Score: 46.595 bits (109), Expect = 1.445e-7
Identity = 20/51 (39.22%), Postives = 29/51 (56.86%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            C    + G+C  Y  R+ F+++ G+C QF YG C  N+NNF  +  CE  C
Sbjct: 1575 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 1625

HSP 4 Score: 46.2098 bits (108), Expect = 1.793e-7
Identity = 20/46 (43.48%), Postives = 27/46 (58.70%), Query Frame = 2

Query:   71 ETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            ++G C   F+R+ FD +   C  F Y GC  N NNF +KEEC  +C
Sbjct:  521 DSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAIC 566

HSP 5 Score: 46.2098 bits (108), Expect = 2.016e-7
Identity = 22/60 (36.67%), Postives = 32/60 (53.33%), Query Frame = 2

Query:   29 NFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            N +     +C   +E G C  +  R+ F+   G C  F YGGC  N+N+F T++ECE  C
Sbjct:  853 NLAAQIEEKCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 912

HSP 6 Score: 44.669 bits (104), Expect = 6.669e-7
Identity = 20/52 (38.46%), Postives = 28/52 (53.85%), Query Frame = 2

Query:   53 ECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            EC      G C   F RY ++    +C QF Y GC  N NN++++E CE+ C
Sbjct:  201 ECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 252

HSP 7 Score: 43.5134 bits (101), Expect = 1.597e-6
Identity = 20/53 (37.74%), Postives = 26/53 (49.06%), Query Frame = 2

Query:   50 PECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            P C   +  G C    +RY +D     C  F Y GC  N NNF + E+C+ LC
Sbjct: 2138 PLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLC 2190

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Celegans
Match: mlt-11 (pep chromosome:WBcel235:V:20667211:20678513:1 gene:WBGene00012186.1 transcript:W01F3.3e.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mlt-11)

HSP 1 Score: 58.9214 bits (141), Expect = 7.084e-12
Identity = 27/64 (42.19%), Postives = 38/64 (59.38%), Query Frame = 2

Query:   17 IVTFNFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            +V    S +RA  C+   E G CD Y +R+ F+  L EC  F +GGC  N+NNF+  E+CE+ C
Sbjct:  526 VVGLQTSGARATRCSLPKERGPCDKYELRFYFNADLNECKYFFWGGCEGNQNNFERVEDCESAC 589

HSP 2 Score: 56.225 bits (134), Expect = 5.412e-11
Identity = 26/59 (44.07%), Postives = 30/59 (50.85%), Query Frame = 2

Query:   38 ESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN 214
            +S    C   IE G C   F R+ FD  +  C  F YGGC  N NNF T +EC N C N
Sbjct:  860 KSPVATCEADIEVGECAGVFSRFAFDKSINACRSFTYGGCGGNANNFATLQECTNKCVN 918

HSP 3 Score: 46.595 bits (109), Expect = 1.724e-7
Identity = 20/51 (39.22%), Postives = 29/51 (56.86%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            C    + G+C  Y  R+ F+++ G+C QF YG C  N+NNF  +  CE  C
Sbjct: 1651 CAMPPDAGVCTNYTPRWFFNSQTGQCEQFAYGSCGGNENNFFDRNTCERKC 1701

HSP 4 Score: 46.2098 bits (108), Expect = 2.118e-7
Identity = 21/51 (41.18%), Postives = 28/51 (54.90%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            C    ++G C   F+R+ FD +   C  F Y GC  N NNF +KEEC  +C
Sbjct:  737 CLHPRDSGNCRGQFVRWFFDDEKKNCDVFTYTGCQGNGNNFASKEECMAIC 787

HSP 5 Score: 45.8246 bits (107), Expect = 2.406e-7
Identity = 22/60 (36.67%), Postives = 32/60 (53.33%), Query Frame = 2

Query:   29 NFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            N +     +C   +E G C  +  R+ F+   G C  F YGGC  N+N+F T++ECE  C
Sbjct: 1074 NLAAQIEEKCLQPVEPGPCKNFADRWYFNVDDGTCHPFKYGGCAGNRNHFFTQKECEVHC 1133

HSP 6 Score: 44.669 bits (104), Expect = 7.805e-7
Identity = 20/52 (38.46%), Postives = 28/52 (53.85%), Query Frame = 2

Query:   53 ECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            EC      G C   F RY ++    +C QF Y GC  N NN++++E CE+ C
Sbjct:  422 ECVGVSLPGPCHGSFQRYFYNEDSQKCEQFTYSGCGGNGNNYESREACEDRC 473

HSP 7 Score: 43.5134 bits (101), Expect = 1.850e-6
Identity = 20/53 (37.74%), Postives = 26/53 (49.06%), Query Frame = 2

Query:   50 PECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            P C   +  G C    +RY +D     C  F Y GC  N NNF + E+C+ LC
Sbjct: 2214 PLCIQPVVKGSCQEAHLRYYYDRVTDSCRLFEYSGCDGNANNFGSLEDCQRLC 2266

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Fly
Match: CG3604 (gene:FBgn0031562 transcript:FBtr0077545)

HSP 1 Score: 60.077 bits (144), Expect = 3.445e-13
Identity = 29/61 (47.54%), Postives = 38/61 (62.30%), Query Frame = 2

Query:   29 NFSESRAPE-CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            + SE   PE C+   ETG C A F RY ++     C +F+YGGC  NKNNF++KE+CE  C
Sbjct:   45 DASELTVPEDCHQPKETGRCFALFYRYAYNVDTQSCEEFVYGGCAGNKNNFESKEQCEQAC 105

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Fly
Match: CG3604 (gene:FBgn0031562 transcript:FBtr0346581)

HSP 1 Score: 60.077 bits (144), Expect = 3.445e-13
Identity = 29/61 (47.54%), Postives = 38/61 (62.30%), Query Frame = 2

Query:   29 NFSESRAPE-CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            + SE   PE C+   ETG C A F RY ++     C +F+YGGC  NKNNF++KE+CE  C
Sbjct:   45 DASELTVPEDCHQPKETGRCFALFYRYAYNVDTQSCEEFVYGGCAGNKNNFESKEQCEQAC 105

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Fly
Match: Ppn (gene:FBgn0003137 transcript:FBtr0301838)

HSP 1 Score: 53.9138 bits (128), Expect = 4.450e-10
Identity = 25/62 (40.32%), Postives = 36/62 (58.06%), Query Frame = 2

Query:   29 NFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN 214
            N  E     C    ETG C+ Y ++Y FDT  G C +F YGGC  N N F+++ EC++ C++
Sbjct: 1603 NVQEPPQKACGLPKETGTCNNYSVKYYFDTSYGGCARFWYGGCDGNDNRFESEAECKDTCQD 1664

HSP 2 Score: 48.521 bits (114), Expect = 3.538e-8
Identity = 21/56 (37.50%), Postives = 32/56 (57.14%), Query Frame = 2

Query:   41 SRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            SR   C++    G C  + +++ FD K+G C QF YG C  N N F+T+ +C+  C
Sbjct: 1917 SRQDVCDEEPAPGECSTWVLKWHFDRKIGACRQFYYGNCGGNGNRFETENDCQQRC 1972

HSP 3 Score: 46.595 bits (109), Expect = 1.782e-7
Identity = 19/52 (36.54%), Postives = 28/52 (53.85%), Query Frame = 2

Query:   53 ECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            +C+   + G CD + + + ++   G C  F YGGC  N N F T+EEC   C
Sbjct: 2000 QCSQPADPGQCDKWALHWNYNETEGRCQSFYYGGCGGNDNRFATEEECSARC 2051

HSP 4 Score: 46.2098 bits (108), Expect = 2.743e-7
Identity = 21/55 (38.18%), Postives = 29/55 (52.73%), Query Frame = 2

Query:   50 PECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN 214
            P C   +E+G C   F R+ +D +   C  F YGGC  NKNN+ T+  C   C+ 
Sbjct: 1728 PTCEQPVESGPCAGNFERWYYDNETDICRPFTYGGCKGNKNNYPTEHACNYNCRQ 1782

HSP 5 Score: 41.9726 bits (97), Expect = 8.582e-6
Identity = 23/59 (38.98%), Postives = 31/59 (52.54%), Query Frame = 2

Query:   53 ECNDTIET-------GLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            ECND   T       G C     R+ FD + GEC +F + GC  N+NNF ++ +C N C
Sbjct: 2120 ECNDAQTTCALPPVRGRCSDLSRRWYFDERSGECHEFEFTGCRGNRNNFVSQSDCLNFC 2178

HSP 6 Score: 41.9726 bits (97), Expect = 8.926e-6
Identity = 19/46 (41.30%), Postives = 24/46 (52.17%), Query Frame = 2

Query:   71 ETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            +TG C     ++ F      C+ F Y GC  NKNNF T E CE+ C
Sbjct: 1795 QTGDCSEKLAKWHFSESEKRCVPFYYSGCGGNKNNFPTLESCEDHC 1840

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Fly
Match: Ppn (gene:FBgn0003137 transcript:FBtr0301839)

HSP 1 Score: 53.9138 bits (128), Expect = 4.451e-10
Identity = 25/62 (40.32%), Postives = 36/62 (58.06%), Query Frame = 2

Query:   29 NFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN 214
            N  E     C    ETG C+ Y ++Y FDT  G C +F YGGC  N N F+++ EC++ C++
Sbjct: 1603 NVQEPPQKACGLPKETGTCNNYSVKYYFDTSYGGCARFWYGGCDGNDNRFESEAECKDTCQD 1664

HSP 2 Score: 48.521 bits (114), Expect = 3.680e-8
Identity = 21/56 (37.50%), Postives = 32/56 (57.14%), Query Frame = 2

Query:   41 SRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            SR   C++    G C  + +++ FD K+G C QF YG C  N N F+T+ +C+  C
Sbjct: 1860 SRQDVCDEEPAPGECSTWVLKWHFDRKIGACRQFYYGNCGGNGNRFETENDCQQRC 1915

HSP 3 Score: 46.595 bits (109), Expect = 1.909e-7
Identity = 19/53 (35.85%), Postives = 28/53 (52.83%), Query Frame = 2

Query:   53 ECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            +C+   + G CD + + + ++   G C  F YGGC  N N F T+EEC   C 
Sbjct: 1943 QCSQPADPGQCDKWALHWNYNETEGRCQSFYYGGCGGNDNRFATEEECSARCS 1995

HSP 4 Score: 46.2098 bits (108), Expect = 2.743e-7
Identity = 21/55 (38.18%), Postives = 29/55 (52.73%), Query Frame = 2

Query:   50 PECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN 214
            P C   +E+G C   F R+ +D +   C  F YGGC  NKNN+ T+  C   C+ 
Sbjct: 1728 PTCEQPVESGPCAGNFERWYYDNETDICRPFTYGGCKGNKNNYPTEHACNYNCRQ 1782

HSP 5 Score: 45.0542 bits (105), Expect = 5.667e-7
Identity = 20/51 (39.22%), Postives = 31/51 (60.78%), Query Frame = 2

Query:   68 IETGLCDAYFI---RYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            + TG C+   +   R+ +D + G C+ FIY GC  N+NNF++ E C N C+
Sbjct: 2265 VATGRCNGPSVHERRWYYDDEAGNCVSFIYAGCSGNQNNFRSFEACTNQCR 2315

HSP 6 Score: 41.9726 bits (97), Expect = 8.927e-6
Identity = 23/59 (38.98%), Postives = 31/59 (52.54%), Query Frame = 2

Query:   53 ECNDTIET-------GLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            ECND   T       G C     R+ FD + GEC +F + GC  N+NNF ++ +C N C
Sbjct: 2063 ECNDAQTTCALPPVRGRCSDLSRRWYFDERSGECHEFEFTGCRGNRNNFVSQSDCLNFC 2121

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Fly
Match: Ppn (gene:FBgn0003137 transcript:FBtr0301837)

HSP 1 Score: 53.9138 bits (128), Expect = 4.451e-10
Identity = 25/62 (40.32%), Postives = 36/62 (58.06%), Query Frame = 2

Query:   29 NFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN 214
            N  E     C    ETG C+ Y ++Y FDT  G C +F YGGC  N N F+++ EC++ C++
Sbjct: 1603 NVQEPPQKACGLPKETGTCNNYSVKYYFDTSYGGCARFWYGGCDGNDNRFESEAECKDTCQD 1664

HSP 2 Score: 48.521 bits (114), Expect = 3.645e-8
Identity = 21/56 (37.50%), Postives = 32/56 (57.14%), Query Frame = 2

Query:   41 SRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            SR   C++    G C  + +++ FD K+G C QF YG C  N N F+T+ +C+  C
Sbjct: 1917 SRQDVCDEEPAPGECSTWVLKWHFDRKIGACRQFYYGNCGGNGNRFETENDCQQRC 1972

HSP 3 Score: 46.595 bits (109), Expect = 1.909e-7
Identity = 19/52 (36.54%), Postives = 28/52 (53.85%), Query Frame = 2

Query:   53 ECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            +C+   + G CD + + + ++   G C  F YGGC  N N F T+EEC   C
Sbjct: 2000 QCSQPADPGQCDKWALHWNYNETEGRCQSFYYGGCGGNDNRFATEEECSARC 2051

HSP 4 Score: 46.2098 bits (108), Expect = 2.798e-7
Identity = 21/55 (38.18%), Postives = 29/55 (52.73%), Query Frame = 2

Query:   50 PECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN 214
            P C   +E+G C   F R+ +D +   C  F YGGC  NKNN+ T+  C   C+ 
Sbjct: 1728 PTCEQPVESGPCAGNFERWYYDNETDICRPFTYGGCKGNKNNYPTEHACNYNCRQ 1782

HSP 5 Score: 45.0542 bits (105), Expect = 5.838e-7
Identity = 20/51 (39.22%), Postives = 31/51 (60.78%), Query Frame = 2

Query:   68 IETGLCDAYFI---RYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            + TG C+   +   R+ +D + G C+ FIY GC  N+NNF++ E C N C+
Sbjct: 2322 VATGRCNGPSVHERRWYYDDEAGNCVSFIYAGCSGNQNNFRSFEACTNQCR 2372

HSP 6 Score: 41.9726 bits (97), Expect = 9.106e-6
Identity = 23/59 (38.98%), Postives = 31/59 (52.54%), Query Frame = 2

Query:   53 ECNDTIET-------GLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            ECND   T       G C     R+ FD + GEC +F + GC  N+NNF ++ +C N C
Sbjct: 2120 ECNDAQTTCALPPVRGRCSDLSRRWYFDERSGECHEFEFTGCRGNRNNFVSQSDCLNFC 2178

HSP 7 Score: 41.9726 bits (97), Expect = 9.471e-6
Identity = 19/46 (41.30%), Postives = 24/46 (52.17%), Query Frame = 2

Query:   71 ETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            +TG C     ++ F      C+ F Y GC  NKNNF T E CE+ C
Sbjct: 1795 QTGDCSEKLAKWHFSESEKRCVPFYYSGCGGNKNNFPTLESCEDHC 1840

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Zebrafish
Match: aplp2 (amyloid beta (A4) precursor-like protein 2 [Source:ZFIN;Acc:ZDB-GENE-061009-28])

HSP 1 Score: 58.5362 bits (140), Expect = 1.712e-11
Identity = 24/52 (46.15%), Postives = 34/52 (65.38%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            C+   ETG C A   R+ FD +  +C++FIYGGC  N+NNF ++E C  +CK
Sbjct:  315 CSLEAETGPCRASMPRWHFDMQQRKCVRFIYGGCAGNRNNFDSEEYCMVVCK 366

[Close]

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            C+   ETG C A   R+ FD +  +C++FIYGGC  N+NNF ++E C  +CK
Sbjct:  315 CSLEAETGPCRASMPRWHFDMQQRKCVRFIYGGCAGNRNNFDSEEYCMVVCK 366

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Zebrafish
Match: wfikkn2b (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2b [Source:ZFIN;Acc:ZDB-GENE-110414-1])

HSP 1 Score: 55.8398 bits (133), Expect = 1.281e-10
Identity = 24/54 (44.44%), Postives = 33/54 (61.11%), Query Frame = 2

Query:   47 APECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            +  C   I  G C AY +R+ + + L +C  FIYGGC  N+NNFK+K  CE+ C
Sbjct:  372 SASCMLPIHKGPCKAYELRWAYSSTLHQCRLFIYGGCKANENNFKSKRSCEDAC 425

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Zebrafish
Match: spint1b (serine peptidase inhibitor, Kunitz type 1 b [Source:ZFIN;Acc:ZDB-GENE-071218-1])

HSP 1 Score: 55.0694 bits (131), Expect = 2.225e-10
Identity = 27/67 (40.30%), Postives = 38/67 (56.72%), Query Frame = 2

Query:   14 IIVTFNFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN 214
            +IV    SE     C    +TG C A FIR+ ++     C QFI+GGC  N NN+ ++ EC+N C+N
Sbjct:  221 VIVLVLTSEQSERHCLTPKKTGPCRASFIRWNYNAASRRCEQFIFGGCMENSNNYLSETECQNACEN 287

HSP 2 Score: 48.1358 bits (113), Expect = 7.158e-8
Identity = 22/61 (36.07%), Postives = 32/61 (52.46%), Query Frame = 2

Query:   26 FNFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
             N   ++   C D   TG C A+F  + +D    +C  F YGGC  N+NNF+T ++C   C
Sbjct:  353 LNIEVNKKAHCTDPPATGPCRAHFHHWYYDPLSKKCHSFTYGGCDGNRNNFETADKCMKNC 413

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Zebrafish
Match: col28a2a (collagen, type XXVIII, alpha 2a [Source:ZFIN;Acc:ZDB-GENE-030131-4444])

HSP 1 Score: 55.0694 bits (131), Expect = 2.330e-10
Identity = 22/53 (41.51%), Postives = 29/53 (54.72%), Query Frame = 2

Query:   50 PECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            P C  +   G C  Y IR+ ++ +   C QF YGGC  N N F T+EEC+  C
Sbjct: 1134 PRCQLSFVQGSCRNYVIRWYYEKQSNSCAQFWYGGCDGNDNRFHTEEECKTTC 1186

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Xenopus
Match: itch (itchy E3 ubiquitin protein ligase [Source:Xenbase;Acc:XB-GENE-976485])

HSP 1 Score: 56.9954 bits (136), Expect = 7.237e-11
Identity = 24/63 (38.10%), Postives = 37/63 (58.73%), Query Frame = 2

Query:   23 TFNFSESRAPE-CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            T N  ++  PE CN   E G C  + +++ +D +   C +F YGGC  N+N F T++ECE +C
Sbjct: 2997 TANSPQTTGPEVCNLQKEEGTCRDFVLKWYYDPETKSCARFWYGGCGGNENRFSTQKECEKIC 3059

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Xenopus
Match: WFIKKN2 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 [Source:NCBI gene;Acc:100486381])

HSP 1 Score: 56.6102 bits (135), Expect = 8.596e-11
Identity = 25/51 (49.02%), Postives = 34/51 (66.67%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            CN     G+C AY  R+ ++ +L +C  FIYGGC  N NNF+TKE CE++C
Sbjct:  370 CNFPALQGMCKAYKPRWAYNKQLKQCQSFIYGGCGGNDNNFETKETCEDMC 420

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Xenopus
Match: APLP2 (amyloid beta (A4) precursor-like protein 2 [Source:NCBI gene;Acc:100380132])

HSP 1 Score: 54.6842 bits (130), Expect = 4.231e-10
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            C+    TG C A   R+ FD    +C++FIYGGC  N+NNF++++ C  +CK
Sbjct:  297 CSQEAMTGPCRAMMPRWYFDLGQKKCVRFIYGGCGGNRNNFESEDYCMAVCK 348

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Xenopus
Match: APLP2 (amyloid beta (A4) precursor-like protein 2 [Source:NCBI gene;Acc:100380132])

HSP 1 Score: 54.6842 bits (130), Expect = 4.233e-10
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            C+    TG C A   R+ FD    +C++FIYGGC  N+NNF++++ C  +CK
Sbjct:  297 CSQEAMTGPCRAMMPRWYFDLGQKKCVRFIYGGCGGNRNNFESEDYCMAVCK 348

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Xenopus
Match: APLP2 (amyloid beta (A4) precursor-like protein 2 [Source:NCBI gene;Acc:100380132])

HSP 1 Score: 54.6842 bits (130), Expect = 4.235e-10
Identity = 22/52 (42.31%), Postives = 33/52 (63.46%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            C+    TG C A   R+ FD    +C++FIYGGC  N+NNF++++ C  +CK
Sbjct:  297 CSQEAMTGPCRAMMPRWYFDLGQKKCVRFIYGGCGGNRNNFESEDYCMAVCK 348

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Mouse
Match: Aplp2 (amyloid beta (A4) precursor-like protein 2 [Source:MGI Symbol;Acc:MGI:88047])

HSP 1 Score: 57.7658 bits (138), Expect = 2.811e-11
Identity = 23/52 (44.23%), Postives = 34/52 (65.38%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            C+    TG C A   R+ FD   G+C++FIYGGC  N+NNF++++ C  +CK
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCK 361

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Mouse
Match: Aplp2 (amyloid beta (A4) precursor-like protein 2 [Source:MGI Symbol;Acc:MGI:88047])

HSP 1 Score: 56.9954 bits (136), Expect = 4.924e-11
Identity = 23/52 (44.23%), Postives = 34/52 (65.38%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            C+    TG C A   R+ FD   G+C++FIYGGC  N+NNF++++ C  +CK
Sbjct:  310 CSQEAMTGPCRAVMPRWYFDLSKGKCVRFIYGGCGGNRNNFESEDYCMAVCK 361

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Mouse
Match: App (amyloid beta (A4) precursor protein [Source:MGI Symbol;Acc:MGI:88059])

HSP 1 Score: 56.225 bits (134), Expect = 1.171e-10
Identity = 23/46 (50.00%), Postives = 29/46 (63.04%), Query Frame = 2

Query:   71 ETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            ETG C A   R+ FD   G+C+ F YGGC  N+NNF T+E C  +C
Sbjct:  296 ETGPCRAMISRWYFDVTEGKCVPFFYGGCGGNRNNFDTEEYCMAVC 341

[Close]

Query:   71 ETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            ETG C A   R+ FD   G+C+ F YGGC  N+NNF T+E C  +C
Sbjct:  296 ETGPCRAMISRWYFDVTEGKCVPFFYGGCGGNRNNFDTEEYCMAVC 341

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Mouse
Match: App (amyloid beta (A4) precursor protein [Source:MGI Symbol;Acc:MGI:88059])

HSP 1 Score: 56.225 bits (134), Expect = 1.172e-10
Identity = 23/46 (50.00%), Postives = 29/46 (63.04%), Query Frame = 2

Query:   71 ETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            ETG C A   R+ FD   G+C+ F YGGC  N+NNF T+E C  +C
Sbjct:  296 ETGPCRAMISRWYFDVTEGKCVPFFYGGCGGNRNNFDTEEYCMAVC 341

[Close]

BLAST of Kunitz-type protease inhibitor vs. UniProt/SwissProt
Match: sp|P81906|ISPI2_GALME (Inducible serine protease inhibitor 2 (Fragment) OS=Galleria mellonella OX=7137 PE=1 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 6.063e-14
Identity = 28/50 (56.00%), Postives = 35/50 (70.00%), Query Frame = 2

Query:   50 PECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECE 199
            P+C   +ETG+C A   R+G+DTKL EC QF+YGGC  N+NNFK  E C 
Sbjct:    3 PKCTLPLETGICRAELHRFGYDTKLKECTQFVYGGCHHNENNFKKLEVCR 52

[Close]

BLAST of Kunitz-type protease inhibitor vs. UniProt/SwissProt
Match: sp|P0DJ50|VKT31_MESMA (Kunitz-type serine protease inhibitor BmKTT-2 OS=Mesobuthus martensii OX=34649 PE=2 SV=2)

HSP 1 Score: 61.2326 bits (147), Expect = 3.463e-13
Identity = 29/68 (42.65%), Postives = 40/68 (58.82%), Query Frame = 2

Query:    8 FVIIVTFNFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
             ++ V    S++   +C    +TG C AYFIRY ++ K GEC +F+YGGC  N NNF TK +C   C 
Sbjct:   10 IILSVVCTISDAEGVDCTLPSDTGRCKAYFIRYFYNQKAGECQKFVYGGCEGNSNNFLTKSDCCKQCS 77

[Close]

BLAST of Kunitz-type protease inhibitor vs. UniProt/SwissProt
Match: sp|G3LH89|VKT_BOMIG (Kunitz-type serine protease inhibitor Bi-KTI OS=Bombus ignitus OX=130704 PE=2 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 4.227e-13
Identity = 25/59 (42.37%), Postives = 37/59 (62.71%), Query Frame = 2

Query:   35 SESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            S      C  ++ TG C  YF R+G++ ++G+C++FIYGGC  N NNF+  EEC+  C 
Sbjct:   23 SAEVPSHCTLSLATGTCKGYFPRFGYNIEMGKCVEFIYGGCDGNANNFRNLEECQQSCS 81

[Close]

BLAST of Kunitz-type protease inhibitor vs. UniProt/SwissProt
Match: sp|P0DMJ5|VKT_HETCR (PI-stichotoxin-Hcr2e OS=Heteractis crispa OX=175771 PE=1 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 5.434e-13
Identity = 29/70 (41.43%), Postives = 42/70 (60.00%), Query Frame = 2

Query:    5 CFVIIVTFNFSESRAPE-CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            C ++I  F+F  ++A   C +    G C AYF R+ FD++ G+C  FIYGGC  N NNF+T   C  +C+
Sbjct:    8 CLILIAGFSFKSTQAGSICLEPKVVGPCTAYFPRFYFDSETGKCTPFIYGGCEGNGNNFETLHACRAICR 77

[Close]

BLAST of Kunitz-type protease inhibitor vs. UniProt/SwissProt
Match: sp|P81902|CSTI_BOMMO (Trypsin inhibitor OS=Bombyx mori OX=7091 PE=1 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 5.880e-13
Identity = 27/53 (50.94%), Postives = 35/53 (66.04%), Query Frame = 2

Query:   50 PECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            P+C   I+TG C   F RY +D+   +C++FIYGGC  N NNF+T EECE  C
Sbjct:    2 PDCLLPIKTGPCKGSFPRYAYDSSEDKCVEFIYGGCQANANNFETIEECEAAC 54

[Close]

BLAST of Kunitz-type protease inhibitor vs. TrEMBL
Match: A0A0R3WBP8 (BPTI/Kunitz inhibitor domain-containing protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS8078 PE=4 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 3.584e-18
Identity = 33/69 (47.83%), Postives = 47/69 (68.12%), Query Frame = 2

Query:    2 FCFVIIVTFNFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
                ++V  + +E+R   C   IE GLC AY  R+G+D + GEC++FIYGGC  NKN+F +KE+CEN+C
Sbjct:   25 LALFVLVAISLTEARIDPCKLPIEPGLCRAYMPRWGYDQESGECVRFIYGGCGGNKNHFHSKEQCENMC 93

[Close]

BLAST of Kunitz-type protease inhibitor vs. TrEMBL
Match: C7SCV7 (Collagen alpha-3(VI) chain OS=Echinococcus granulosus OX=6210 GN=EGR_07243 PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 8.001e-18
Identity = 33/71 (46.48%), Postives = 48/71 (67.61%), Query Frame = 2

Query:    2 FCFVIIVTFNFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN 214
            F   ++V  +FSE+R   C   ++ G C AYF R+GF  + GEC++FIYGGC  NKN F +KE+CE++C +
Sbjct:    5 FALFLLVAISFSEARIDPCKLPLDPGFCRAYFPRWGFHQESGECVRFIYGGCGGNKNQFHSKEQCESMCGH 75

[Close]

BLAST of Kunitz-type protease inhibitor vs. TrEMBL
Match: A0A0R3X360 (BPTI/Kunitz inhibitor domain-containing protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS7747 PE=4 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 2.362e-15
Identity = 31/58 (53.45%), Postives = 43/58 (74.14%), Query Frame = 2

Query:   35 SESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            +E+R   C   IE GLC AY  R+G+D + G+C++FIYGGC  NKN+F +KE+CEN+C
Sbjct:   16 TEARIDPCKLPIEPGLCRAYIPRWGYDQESGDCVRFIYGGCGGNKNHFHSKEQCENMC 73

[Close]

BLAST of Kunitz-type protease inhibitor vs. TrEMBL
Match: A0A0R3SS90 (BPTI/Kunitz inhibitor domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS8135 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.711e-15
Identity = 32/51 (62.75%), Postives = 37/51 (72.55%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            CN  I+TG C AYF R+GFD  +G CI FIYGGC  N+NNF + EECE LC
Sbjct:    5 CNQPIDTGRCMAYFPRWGFDRSMGRCIPFIYGGCKGNQNNFHSMEECETLC 55

[Close]

BLAST of Kunitz-type protease inhibitor vs. TrEMBL
Match: A0A564YH51 (BPTI/Kunitz inhibitor domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS6499 PE=4 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 3.402e-15
Identity = 35/68 (51.47%), Postives = 42/68 (61.76%), Query Frame = 2

Query:    5 CFVIIVTFNFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            C + I   N    +   CN  I+TG C AYF R+GFD  +G CI FIYGGC  N+NNF + EECE LC
Sbjct:   11 CVLAICHCNNRMDQFEACNQPIDTGRCMAYFPRWGFDRSMGRCIPFIYGGCKGNQNNFHSMEECETLC 78

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Cavefish
Match: col28a2b (collagen, type XXVIII, alpha 2b [Source:ZFIN;Acc:ZDB-GENE-130530-762])

HSP 1 Score: 60.8474 bits (146), Expect = 1.724e-12
Identity = 24/53 (45.28%), Postives = 32/53 (60.38%), Query Frame = 2

Query:   50 PECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            P C  T++ G C  Y IR+ +D +   C QF YGGC  N+N F T+EEC+  C
Sbjct: 1081 PRCELTLDQGPCREYNIRWYYDKQANACAQFWYGGCDGNRNRFDTEEECKKTC 1133

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Cavefish
Match: aplp2 (amyloid beta precursor like protein 2 [Source:NCBI gene;Acc:103022515])

HSP 1 Score: 58.9214 bits (141), Expect = 9.161e-12
Identity = 24/52 (46.15%), Postives = 34/52 (65.38%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            C+   ETG C A   R+ FD +  +C++FIYGGC  N+NNF ++E C  +CK
Sbjct:  315 CSLEAETGPCRASMPRWHFDMQQRKCVRFIYGGCAGNRNNFDSEEYCMAVCK 366

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Cavefish
Match: aplp2 (amyloid beta precursor like protein 2 [Source:NCBI gene;Acc:103022515])

HSP 1 Score: 58.9214 bits (141), Expect = 9.166e-12
Identity = 24/52 (46.15%), Postives = 34/52 (65.38%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            C+   ETG C A   R+ FD +  +C++FIYGGC  N+NNF ++E C  +CK
Sbjct:  315 CSLEAETGPCRASMPRWHFDMQQRKCVRFIYGGCAGNRNNFDSEEYCMAVCK 366

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Cavefish
Match: aplp2 (amyloid beta precursor like protein 2 [Source:NCBI gene;Acc:103022515])

HSP 1 Score: 58.9214 bits (141), Expect = 9.170e-12
Identity = 24/52 (46.15%), Postives = 34/52 (65.38%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            C+   ETG C A   R+ FD +  +C++FIYGGC  N+NNF ++E C  +CK
Sbjct:  315 CSLEAETGPCRASMPRWHFDMQQRKCVRFIYGGCAGNRNNFDSEEYCMAVCK 366

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Cavefish
Match: col28a2a (collagen alpha-1(XXVIII) chain-like [Source:NCBI gene;Acc:103029985])

HSP 1 Score: 58.151 bits (139), Expect = 2.026e-11
Identity = 22/53 (41.51%), Postives = 31/53 (58.49%), Query Frame = 2

Query:   50 PECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            P C+ ++  G C  Y IR+ +D     C QF YGGC  N+N F+T+ EC+  C
Sbjct: 1192 PRCSLSLNQGPCREYLIRWYYDKAANSCAQFWYGGCEGNENRFETEAECKKTC 1244

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Sea Lamprey
Match: WFIKKN1 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 [Source:HGNC Symbol;Acc:HGNC:30912])

HSP 1 Score: 48.9062 bits (115), Expect = 6.294e-9
Identity = 19/51 (37.25%), Postives = 33/51 (64.71%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            C+  ++ G C  + +R+ ++  +  C  FIYGGC  N+NNF+++E CE+ C
Sbjct:  374 CSLPVQQGPCKQWALRWAYNPLIKRCQSFIYGGCEGNENNFQSQELCEDNC 424

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Sea Lamprey
Match: tfpi2 (tissue factor pathway inhibitor 2 [Source:ZFIN;Acc:ZDB-GENE-060503-38])

HSP 1 Score: 47.7506 bits (112), Expect = 1.163e-8
Identity = 21/47 (44.68%), Postives = 27/47 (57.45%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEEC 196
            C    ETG C A+  RY ++  LG C+ F+YGGC  N N F+  E C
Sbjct:   87 CRPDAETGRCRAHITRYFYNMTLGMCMPFVYGGCGGNNNRFEDMESC 133

HSP 2 Score: 46.9802 bits (110), Expect = 2.813e-8
Identity = 24/58 (41.38%), Postives = 30/58 (51.72%), Query Frame = 2

Query:   38 ESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            E +   C    + G C A F RY F+     C  FIYGGC  N+NNF+T +EC   C 
Sbjct:   21 EDQRVTCTLPAKPGDCRAAFPRYFFNRSTLRCESFIYGGCHSNENNFETMQECRAACS 78

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Sea Lamprey
Match: WFIKKN1 (WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 [Source:HGNC Symbol;Acc:HGNC:30912])

HSP 1 Score: 47.7506 bits (112), Expect = 1.508e-8
Identity = 21/60 (35.00%), Postives = 34/60 (56.67%), Query Frame = 2

Query:   35 SESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN 214
            +E  A EC++  +   C +   ++ +D+ L  C  F+YGGC  N+N F T E C+  C+N
Sbjct:  334 AEFPASECHEPPDVTHCGSARTKWYYDSGLNACDTFVYGGCDSNRNRFDTYEGCKRWCQN 393

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005038.1 (pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 gene:ENSPMAG00000004579.1 transcript:ENSPMAT00000005038.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 44.2838 bits (103), Expect = 2.405e-7
Identity = 24/55 (43.64%), Postives = 29/55 (52.73%), Query Frame = 2

Query:   47 APE-CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            AP+ C   +E G+C A   RY F+   G C +F YGGC  N NNFK    C   C
Sbjct:   71 APKACRLDMEVGVCRALLRRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHC 125

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005043.1 (pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 gene:ENSPMAG00000004579.1 transcript:ENSPMAT00000005043.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 43.8986 bits (102), Expect = 2.730e-7
Identity = 24/55 (43.64%), Postives = 29/55 (52.73%), Query Frame = 2

Query:   47 APE-CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            AP+ C   +E G+C A   RY F+   G C +F YGGC  N NNFK    C   C
Sbjct:   70 APKACRLDMEVGVCRALLRRYFFNMTSGSCEEFGYGGCQGNLNNFKDAASCYQHC 124

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Nematostella
Match: EDO41696 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3T1])

HSP 1 Score: 51.9878 bits (123), Expect = 3.659e-11
Identity = 25/52 (48.08%), Postives = 32/52 (61.54%), Query Frame = 2

Query:   53 ECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            EC     TG C AYF R+ ++    +C QF+YGGC  N NNF++K ECE  C
Sbjct:    1 ECLQPKLTGPCRAYFERWFYNQTSRKCKQFVYGGCQGNSNNFESKAECEKKC 52

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Nematostella
Match: EDO26263 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3T1])

HSP 1 Score: 51.9878 bits (123), Expect = 3.659e-11
Identity = 25/52 (48.08%), Postives = 32/52 (61.54%), Query Frame = 2

Query:   53 ECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            EC     TG C AYF R+ ++    +C QF+YGGC  N NNF++K ECE  C
Sbjct:    1 ECLQPKLTGPCRAYFERWFYNQTSRKCKQFVYGGCQGNSNNFESKAECEKKC 52

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Nematostella
Match: EDO41921 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S321])

HSP 1 Score: 49.2914 bits (116), Expect = 4.391e-10
Identity = 23/47 (48.94%), Postives = 31/47 (65.96%), Query Frame = 2

Query:   71 ETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            + G C AY  R+ F+ +  EC +FIYGGC  N+N F TK EC+ +CK
Sbjct:    1 DPGPCKAYMPRFYFEIEKKECQEFIYGGCGGNENRFFTKRECQRICK 47

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Nematostella
Match: EDO38466 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCV3])

HSP 1 Score: 49.2914 bits (116), Expect = 6.453e-10
Identity = 22/45 (48.89%), Postives = 27/45 (60.00%), Query Frame = 2

Query:   74 TGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            TG C A FIR+ +D +  EC  F YGGC  N NNF++K  C   C
Sbjct:   21 TGPCMARFIRWHYDMRSSECKMFTYGGCQGNANNFESKAACLKKC 65

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Nematostella
Match: EDO26072 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TCY8])

HSP 1 Score: 49.2914 bits (116), Expect = 7.356e-10
Identity = 22/45 (48.89%), Postives = 27/45 (60.00%), Query Frame = 2

Query:   74 TGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            TG C A FIR+ +D +  EC  F YGGC  N NNF++K  C   C
Sbjct:   21 TGPCMARFIRWHYDMRSSECKMFTYGGCQGNANNFESKAACLKKC 65

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Medaka
Match: APP (amyloid-beta A4 protein [Source:NCBI gene;Acc:101162104])

HSP 1 Score: 61.2326 bits (147), Expect = 1.585e-12
Identity = 25/53 (47.17%), Postives = 35/53 (66.04%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN 214
            C    ETG C A   R+ FD + G C+QFIYGGC  N+NNF+++E C ++C +
Sbjct:  330 CWADAETGPCRALLPRWFFDREEGRCVQFIYGGCGGNRNNFESEEYCMSVCSS 382

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Medaka
Match: col28a2a (collagen alpha-1(XXVIII) chain [Source:NCBI gene;Acc:101157899])

HSP 1 Score: 60.4622 bits (145), Expect = 2.679e-12
Identity = 23/53 (43.40%), Postives = 33/53 (62.26%), Query Frame = 2

Query:   50 PECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            P C+ T++ G C  Y IR+ +D +   C QF YGGC  N+N F+T+ EC+  C
Sbjct:  968 PRCSITLDQGTCRDYVIRWYYDQQANACAQFWYGGCGGNENRFETESECKKTC 1020

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Medaka
Match: aplp2 (amyloid beta precursor like protein 2 [Source:NCBI gene;Acc:101168561])

HSP 1 Score: 57.7658 bits (138), Expect = 2.259e-11
Identity = 24/52 (46.15%), Postives = 32/52 (61.54%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            C    ETG C A   R+ FD    +C++FIYGGC  N+NNF ++E C  +CK
Sbjct:  314 CTLEAETGPCRASMPRWHFDLSQRKCVRFIYGGCAGNRNNFDSEEYCMAVCK 365

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Medaka
Match: aplp2 (amyloid beta precursor like protein 2 [Source:NCBI gene;Acc:101168561])

HSP 1 Score: 57.7658 bits (138), Expect = 2.260e-11
Identity = 24/52 (46.15%), Postives = 32/52 (61.54%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            C    ETG C A   R+ FD    +C++FIYGGC  N+NNF ++E C  +CK
Sbjct:  314 CTLEAETGPCRASMPRWHFDLSQRKCVRFIYGGCAGNRNNFDSEEYCMAVCK 365

[Close]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Medaka
Match: aplp2 (amyloid beta precursor like protein 2 [Source:NCBI gene;Acc:101168561])

HSP 1 Score: 57.7658 bits (138), Expect = 2.261e-11
Identity = 24/52 (46.15%), Postives = 32/52 (61.54%), Query Frame = 2

Query:   56 CNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            C    ETG C A   R+ FD    +C++FIYGGC  N+NNF ++E C  +CK
Sbjct:  314 CTLEAETGPCRASMPRWHFDLSQRKCVRFIYGGCAGNRNNFDSEEYCMAVCK 365

[Close]

BLAST of Kunitz-type protease inhibitor vs. Planmine SMEST
Match: SMESG000037991.1 (SMESG000037991.1)

HSP 1 Score: 144.821 bits (364), Expect = 2.097e-47
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 2

Query:    2 FCFVIIVTFNFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN 214
            FCFVIIVTFNFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN
Sbjct:    7 FCFVIIVTFNFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCKN 77

[Close]

BLAST of Kunitz-type protease inhibitor vs. Planmine SMEST
Match: SMESG000038055.1 (SMESG000038055.1)

HSP 1 Score: 92.0485 bits (227), Expect = 1.783e-26
Identity = 39/67 (58.21%), Postives = 49/67 (73.13%), Query Frame = 2

Query:   11 VIIVTFNFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            + +      E +APECN ++ETGLC AYF+RYGFD KLG+C  F+YGGCPVN NNF T+ ECE  C+
Sbjct:   10 LFVTALQLIEGKAPECNLSLETGLCRAYFVRYGFDVKLGKCRTFVYGGCPVNDNNFHTRAECEERCE 76

[Close]

BLAST of Kunitz-type protease inhibitor vs. Planmine SMEST
Match: SMESG000038052.1 (SMESG000038052.1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.760e-26
Identity = 39/70 (55.71%), Postives = 51/70 (72.86%), Query Frame = 2

Query:    2 FCFVIIVTFNFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLCK 211
            F  + +     +ES++PECN  +ETGLC+AYF+RYGFD KLG+C  F+YGGC VN NNF T+ ECE  C+
Sbjct:    7 FSCIFVAVLQLTESKSPECNLPLETGLCEAYFVRYGFDVKLGKCRTFVYGGCHVNDNNFHTRAECEERCE 76

[Close]

BLAST of Kunitz-type protease inhibitor vs. Planmine SMEST
Match: SMESG000037994.1 (SMESG000037994.1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.743e-20
Identity = 37/69 (53.62%), Postives = 45/69 (65.22%), Query Frame = 2

Query:    2 FCFVIIVTFNFSESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
            F  +  V F+  ESRAPECN+  +TG+C AYF R+ F+ + G C  FIYGGC  N NNF T EECE  C
Sbjct:    7 FTLIFCVIFSLGESRAPECNEPKKTGMCAAYFPRFYFNIQSGNCESFIYGGCQANGNNFNTIEECEARC 75

[Close]

BLAST of Kunitz-type protease inhibitor vs. Planmine SMEST
Match: SMESG000037999.1 (SMESG000037999.1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.509e-19
Identity = 35/70 (50.00%), Postives = 51/70 (72.86%), Query Frame = 2

Query:    8 FVIIVTFNFS---ESRAPECNDTIETGLCDAYFIRYGFDTKLGECIQFIYGGCPVNKNNFKTKEECENLC 208
             V++V F+ S   ESR  ECN+  ++G+C+AYF R+ F+++ G+C  FIYGGC  N NNF+T++ECE LC
Sbjct:    6 LVLVVLFSLSQLLESRLKECNEPKQSGMCEAYFKRFYFNSETGKCETFIYGGCGKNGNNFQTEDECEELC 75

The following BLAST results are available for this feature:

BLAST of Kunitz-type protease inhibitor vs. Ensembl Human
BLAST of Kunitz-type protease inhibitor vs. Ensembl Celegans
BLAST of Kunitz-type protease inhibitor vs. Ensembl Fly
BLAST of Kunitz-type protease inhibitor vs. Ensembl Zebrafish
BLAST of Kunitz-type protease inhibitor vs. Ensembl Xenopus
BLAST of Kunitz-type protease inhibitor vs. Ensembl Mouse
BLAST of Kunitz-type protease inhibitor vs. UniProt/SwissProt
BLAST of Kunitz-type protease inhibitor vs. TrEMBL
BLAST of Kunitz-type protease inhibitor vs. Ensembl Cavefish
BLAST of Kunitz-type protease inhibitor vs. Ensembl Sea Lamprey
BLAST of Kunitz-type protease inhibitor vs. Ensembl Yeast
BLAST of Kunitz-type protease inhibitor vs. Ensembl Nematostella
BLAST of Kunitz-type protease inhibitor vs. Ensembl Medaka
BLAST of Kunitz-type protease inhibitor vs. Planmine SMEST

BLAST of Kunitz-type protease inhibitor vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
APLP2	4.343e-11	44.23	amyloid beta precursor like protein 2 [Source:HGNC...	[more]
APLP2	4.343e-11	44.23	amyloid beta precursor like protein 2 [Source:HGNC...	[more]
APLP2	4.787e-11	44.23	amyloid beta precursor like protein 2 [Source:HGNC...	[more]
APLP2	5.178e-11	44.23	amyloid beta precursor like protein 2 [Source:HGNC...	[more]
EPPIN	8.504e-11	49.06	epididymal peptidase inhibitor [Source:HGNC Symbol...	[more]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
mlt-11	2.618e-12	42.19	pep chromosome:WBcel235:V:20667211:20678513:1 gene...	[more]
mlt-11	2.618e-12	42.19	pep chromosome:WBcel235:V:20667211:20678513:1 gene...	[more]
mlt-11	3.277e-12	42.19	pep chromosome:WBcel235:V:20667209:20678513:1 gene...	[more]
mlt-11	5.547e-12	42.19	pep chromosome:WBcel235:V:20667209:20678513:1 gene...	[more]
mlt-11	7.084e-12	42.19	pep chromosome:WBcel235:V:20667211:20678513:1 gene...	[more]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
CG3604	3.445e-13	47.54	gene:FBgn0031562 transcript:FBtr0077545	[more]
CG3604	3.445e-13	47.54	gene:FBgn0031562 transcript:FBtr0346581	[more]
Ppn	4.450e-10	40.32	gene:FBgn0003137 transcript:FBtr0301838	[more]
Ppn	4.451e-10	40.32	gene:FBgn0003137 transcript:FBtr0301839	[more]
Ppn	4.451e-10	40.32	gene:FBgn0003137 transcript:FBtr0301837	[more]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
aplp2	1.712e-11	46.15	amyloid beta (A4) precursor-like protein 2 [Source...	[more]
aplp2	1.712e-11	46.15	amyloid beta (A4) precursor-like protein 2 [Source...	[more]
wfikkn2b	1.281e-10	44.44	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
spint1b	2.225e-10	40.30	serine peptidase inhibitor, Kunitz type 1 b [Sourc...	[more]
col28a2a	2.330e-10	41.51	collagen, type XXVIII, alpha 2a [Source:ZFIN;Acc:Z...	[more]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
itch	7.237e-11	38.10	itchy E3 ubiquitin protein ligase [Source:Xenbase;...	[more]
WFIKKN2	8.596e-11	49.02	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
APLP2	4.231e-10	42.31	amyloid beta (A4) precursor-like protein 2 [Source...	[more]
APLP2	4.233e-10	42.31	amyloid beta (A4) precursor-like protein 2 [Source...	[more]
APLP2	4.235e-10	42.31	amyloid beta (A4) precursor-like protein 2 [Source...	[more]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Aplp2	2.811e-11	44.23	amyloid beta (A4) precursor-like protein 2 [Source...	[more]
Aplp2	4.924e-11	44.23	amyloid beta (A4) precursor-like protein 2 [Source...	[more]
App	1.171e-10	50.00	amyloid beta (A4) precursor protein [Source:MGI Sy...	[more]
App	1.171e-10	50.00	amyloid beta (A4) precursor protein [Source:MGI Sy...	[more]
App	1.172e-10	50.00	amyloid beta (A4) precursor protein [Source:MGI Sy...	[more]

BLAST of Kunitz-type protease inhibitor vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|P81906\|ISPI2_GALME	6.063e-14	56.00	Inducible serine protease inhibitor 2 (Fragment) O...	[more]
sp\|P0DJ50\|VKT31_MESMA	3.463e-13	42.65	Kunitz-type serine protease inhibitor BmKTT-2 OS=M...	[more]
sp\|G3LH89\|VKT_BOMIG	4.227e-13	42.37	Kunitz-type serine protease inhibitor Bi-KTI OS=Bo...	[more]
sp\|P0DMJ5\|VKT_HETCR	5.434e-13	41.43	PI-stichotoxin-Hcr2e OS=Heteractis crispa OX=17577...	[more]
sp\|P81902\|CSTI_BOMMO	5.880e-13	50.94	Trypsin inhibitor OS=Bombyx mori OX=7091 PE=1 SV=1	[more]

BLAST of Kunitz-type protease inhibitor vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0R3WBP8	3.584e-18	47.83	BPTI/Kunitz inhibitor domain-containing protein OS...	[more]
C7SCV7	8.001e-18	46.48	Collagen alpha-3(VI) chain OS=Echinococcus granulo...	[more]
A0A0R3X360	2.362e-15	53.45	BPTI/Kunitz inhibitor domain-containing protein OS...	[more]
A0A0R3SS90	2.711e-15	62.75	BPTI/Kunitz inhibitor domain-containing protein OS...	[more]
A0A564YH51	3.402e-15	51.47	BPTI/Kunitz inhibitor domain-containing protein OS...	[more]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
col28a2b	1.724e-12	45.28	collagen, type XXVIII, alpha 2b [Source:ZFIN;Acc:Z...	[more]
aplp2	9.161e-12	46.15	amyloid beta precursor like protein 2 [Source:NCBI...	[more]
aplp2	9.166e-12	46.15	amyloid beta precursor like protein 2 [Source:NCBI...	[more]
aplp2	9.170e-12	46.15	amyloid beta precursor like protein 2 [Source:NCBI...	[more]
col28a2a	2.026e-11	41.51	collagen alpha-1(XXVIII) chain-like [Source:NCBI g...	[more]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
WFIKKN1	6.294e-9	37.25	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
tfpi2	1.163e-8	44.68	tissue factor pathway inhibitor 2 [Source:ZFIN;Acc...	[more]
WFIKKN1	1.508e-8	35.00	WAP, follistatin/kazal, immunoglobulin, kunitz and...	[more]
ENSPMAT00000005038.1	2.405e-7	43.64	pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 g...	[more]
ENSPMAT00000005043.1	2.730e-7	43.64	pep scaffold:Pmarinus_7.0:GL481015:31246:35903:1 g...	[more]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Kunitz-type protease inhibitor vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO41696	3.659e-11	48.08	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO26263	3.659e-11	48.08	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO41921	4.391e-10	48.94	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO38466	6.453e-10	48.89	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO26072	7.356e-10	48.89	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Kunitz-type protease inhibitor vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
APP	1.585e-12	47.17	amyloid-beta A4 protein [Source:NCBI gene;Acc:1011...	[more]
col28a2a	2.679e-12	43.40	collagen alpha-1(XXVIII) chain [Source:NCBI gene;A...	[more]
aplp2	2.259e-11	46.15	amyloid beta precursor like protein 2 [Source:NCBI...	[more]
aplp2	2.260e-11	46.15	amyloid beta precursor like protein 2 [Source:NCBI...	[more]
aplp2	2.261e-11	46.15	amyloid beta precursor like protein 2 [Source:NCBI...	[more]

BLAST of Kunitz-type protease inhibitor vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000037991.1	2.097e-47	100.00	SMESG000037991.1	[more]
SMESG000038055.1	1.783e-26	58.21	SMESG000038055.1	[more]
SMESG000038052.1	3.760e-26	55.71	SMESG000038052.1	[more]
SMESG000037994.1	1.743e-20	53.62	SMESG000037994.1	[more]
SMESG000037999.1	1.509e-19	50.00	SMESG000037999.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence

transcript sequence

>SMED30027656 ID=SMED30027656|Name=Kunitz-type protease inhibitor|organism=Schmidtea mediterranea sexual|type=transcript|length=215bp
TTTCTGTTTTGTGATTATAGTCACTTTTAATTTCAGTGAAAGTCGAGCCC
CTGAATGCAACGATACTATTGAAACTGGCTTATGCGACGCTTACTTTATC
AGATACGGTTTTGATACAAAATTAGGAGAATGCATACAATTTATATACGG
TGGATGTCCAGTCAATAAAAACAACTTTAAAACAAAAGAAGAATGTGAAA
ATCTTTGTAAAAATT

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000419	prepharyngeal region

Vocabulary: **INTERPRO**
Term	Definition
IPR036880	Kunitz_BPTI_sf
IPR002223	Kunitz_BPTI
IPR020901	Prtase_inh_Kunz-CS

Vocabulary: **molecular function**
Term	Definition
GO:0004867	serine-type endopeptidase inhibitor activity
GO:0030414	peptidase inhibitor activity

Vocabulary: **cellular component**
Term	Definition
GO:0005576	extracellular region

Vocabulary: **biological process**
Term	Definition
GO:0010466	negative regulation of peptidase activity
GO:0010951	negative regulation of endopeptidase activity

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002223	Pancreatic trypsin inhibitor Kunitz domain	PRINTS	PR00759	BASICPTASE	coord: 54..69 score: 44.9 coord: 16..30 score: 27.47 coord: 44..54 score: 53.0
IPR002223	Pancreatic trypsin inhibitor Kunitz domain	SMART	SM00131	KU_2	coord: 17..70 e-value: 1.1E-16 score: 71.5
IPR002223	Pancreatic trypsin inhibitor Kunitz domain	PFAM	PF00014	Kunitz_BPTI	coord: 19..70 e-value: 3.4E-16 score: 59.2
IPR002223	Pancreatic trypsin inhibitor Kunitz domain	PROSITE	PS50279	BPTI_KUNITZ_2	coord: 19..69 score: 13.814
IPR002223	Pancreatic trypsin inhibitor Kunitz domain	CDD	cd00109	KU	coord: 19..70 e-value: 7.37589E-16 score: 62.2674
IPR036880	Pancreatic trypsin inhibitor Kunitz domain superfamily	GENE3D	G3DSA:4.10.410.10		coord: 14..71 e-value: 5.9E-19 score: 69.7
IPR036880	Pancreatic trypsin inhibitor Kunitz domain superfamily	SUPERFAMILY	SSF57362	BPTI-like	coord: 13..70
None	No IPR available	PANTHER	PTHR10083	KUNITZ-TYPE PROTEASE INHIBITOR-RELATED	coord: 17..69
IPR020901	Proteinase inhibitor I2, Kunitz, conserved site	PROSITE	PS00280	BPTI_KUNITZ_1	coord: 47..65

Main menu

Kunitz-type protease inhibitor

Zeng et. al., 2018

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 2

Owlery

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