SMED30027628

Overview
NameSMED30027628
Smed IDSMED30027628
Length (bp)1882
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30027628 (SMED30027628) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30027628 (SMED30027628) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30027628 (SMED30027628) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30027628 (SMED30027628) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30027628

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30027628SMESG000077696.1 SMESG000077694.1 dd_Smed_v4_1292_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30027628SMESG000077696.1 SMESG000077694.1 dd_Smed_v4_1292_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30027628SMESG000077696.1 SMESG000077694.1 dd_Smed_v4_1292_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30027628SMESG000077696.1 SMESG000077694.1 dd_Smed_v4_1292_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30027628SMESG000077696.1 SMESG000077694.1 dd_Smed_v4_1292_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30027628SMESG000077696.1 SMESG000077694.1 dd_Smed_v4_1292_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30027628 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000979:49733..75616 +9153
Homology
[Close]
BLAST of SMED30027628 vs. TrEMBL
Match: A0A3P6HMB2 (Uncharacterized protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS6098 PE=4 SV=1)

HSP 1 Score: 83.5741 bits (205), Expect = 7.995e-13
Identity = 49/135 (36.30%), Postives = 80/135 (59.26%), Query Frame = 2 
Query:  239 EENALCKREKSRIAAKKRRTQENENLSELYISLPVATQ------SKLLSCYDNQFIGNFEKATIIKISAFVLNLYNSLSDLLFDNSFQFDKHPLVSLFSQSIFGLFWCDKTSKLIFSSPGLARLTGLYWCNMIGM 625
            E+ AL ++EKSR+AAK RR QE++ LS L  +LPV+ +      +++ S   N    N EK+ +I+++   L LYNSL+ +L +        PL+SL S+S++GL      S +++++P LA      W N+IG+
Sbjct:   87 EQRALRRKEKSRLAAKLRRNQESDILSCLLRALPVSLENSQQATTEIKSVSSNSNGLNLEKSGVIRMAGQTLFLYNSLAHVLGNKEV-----PLISLLSKSLYGLLVHPTDSTVVYATPPLAEALNWPWINLIGI 216
[Close]
BLAST of SMED30027628 vs. TrEMBL
Match: A0A5K3EMQ1 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 1.091e-12
Identity = 49/135 (36.30%), Postives = 80/135 (59.26%), Query Frame = 2 
Query:  239 EENALCKREKSRIAAKKRRTQENENLSELYISLPVATQ------SKLLSCYDNQFIGNFEKATIIKISAFVLNLYNSLSDLLFDNSFQFDKHPLVSLFSQSIFGLFWCDKTSKLIFSSPGLARLTGLYWCNMIGM 625
            E+ AL ++EKSR+AAK RR QE++ LS L  +LPV+ +      +++ S   N    N EK+ +I+++   L LYNSL+ +L +        PL+SL S+S++GL      S +++++P LA      W N+IG+
Sbjct:  185 EQRALRRKEKSRLAAKLRRNQESDILSCLLRALPVSLENSQQATTEIKSVSSNSNGLNLEKSGVIRMAGQTLFLYNSLAHVLGNKEV-----PLISLLSKSLYGLLVHPTDSTVVYATPPLAEALNWPWINLIGI 314
[Close]
BLAST of SMED30027628 vs. TrEMBL
Match: A0A3P7D7A1 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS14521 PE=4 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 1.561e-12
Identity = 53/140 (37.86%), Postives = 77/140 (55.00%), Query Frame = 2 
Query:  239 EENALCKREKSRIAAKKRRTQENENLSELYISLPV------ATQSKLLSCYDNQFIG------NFEKATIIKISAFVLNLYNSLSDLLFDNSFQFDKHPLVSLFSQSIFGLFWCDKTSKLIFSSPGLARLTGLYWCNMIG 622
            E  AL ++EKSR+AAK RR +E+  LS+L  +LPV      A+ +  L   D   +       + EK+ +I+I+   L LYNSLS +L   S      PL+SL S+SI+GLF   +   + + +P LA   G  W N+IG
Sbjct:  150 EVRALRRKEKSRLAAKVRRNRESNILSQLMHALPVPLDAPSASATSRLRGEDAATVPCMGSCVSLEKSGVIRIAGQTLFLYNSLSQILGTQST-----PLISLLSRSIYGLFVHPRNRTIAYVTPPLAEALGWPWINLIG 284
[Close]
BLAST of SMED30027628 vs. TrEMBL
Match: A0A183TDH3 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 PE=4 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.602e-12
Identity = 53/140 (37.86%), Postives = 77/140 (55.00%), Query Frame = 2 
Query:  239 EENALCKREKSRIAAKKRRTQENENLSELYISLPV------ATQSKLLSCYDNQFIG------NFEKATIIKISAFVLNLYNSLSDLLFDNSFQFDKHPLVSLFSQSIFGLFWCDKTSKLIFSSPGLARLTGLYWCNMIG 622
            E  AL ++EKSR+AAK RR +E+  LS+L  +LPV      A+ +  L   D   +       + EK+ +I+I+   L LYNSLS +L   S      PL+SL S+SI+GLF   +   + + +P LA   G  W N+IG
Sbjct:  150 EVRALRRKEKSRLAAKVRRNRESNILSQLMHALPVPLDAPSASATSRLRGEDAATVPCMGSCVSLEKSGVIRIAGQTLFLYNSLSQILGTQST-----PLISLLSRSIYGLFVHPRNRTIAYVTPPLAEALGWPWINLIG 284
[Close]
BLAST of SMED30027628 vs. TrEMBL
Match: A0A0V0J596 (Uncharacterized protein (Fragment) OS=Schistocephalus solidus OX=70667 GN=TR135927 PE=4 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 2.985e-12
Identity = 53/140 (37.86%), Postives = 77/140 (55.00%), Query Frame = 2 
Query:  239 EENALCKREKSRIAAKKRRTQENENLSELYISLPV------ATQSKLLSCYDNQFIG------NFEKATIIKISAFVLNLYNSLSDLLFDNSFQFDKHPLVSLFSQSIFGLFWCDKTSKLIFSSPGLARLTGLYWCNMIG 622
            E  AL ++EKSR+AAK RR +E+  LS+L  +LPV      A+ +  L   D   +       + EK+ +I+I+   L LYNSLS +L   S      PL+SL S+SI+GLF   +   + + +P LA   G  W N+IG
Sbjct:  301 EVRALRRKEKSRLAAKVRRNRESNILSQLMHALPVPLDAPSASATSRLRGEDAATVPCMGSCVSLEKSGVIRIAGQTLFLYNSLSQILGTQST-----PLISLLSRSIYGLFVHPRNRTIAYVTPPLAEALGWPWINLIG 435
[Close]
BLAST of SMED30027628 vs. Planmine SMEST
Match: SMESG000077694.1 (SMESG000077694.1)

HSP 1 Score: 1144.41 bits (2959), Expect = 0.000e+0
Identity = 574/576 (99.65%), Postives = 575/576 (99.83%), Query Frame = 2 
Query:   44 MCKILSNNKKCEXXXXXXXXXXXXXXXXSGLNDVVSRDFNSCFSNFPDITKNSDNNSTNKFSNCSEENALCKREKSRIAAKKRRTQENENLSELYISLPVATQSKLLSCYDNQFIGNFEKATIIKISAFVLNLYNSLSDLLFDNSFQFDKHPLVSLFSQSIFGLFWCDKTSKLIFSSPGLARLTGLYWCNMIGMPRSSIIVECGSQDSSNMIFLRFMMSALKDRGIFTVNPQKPGNYKQSEESTLEKSQILYNQAKRDFIGKLPCSVWCSQKLPNGITFDLIQPLMIKSESFSHGTKFNQSHKHCPYRLPIFTDMHLREIKRPGHRFTSYHYLDSLCTMSVSGDFYSSLGYLKEDFSKSNLLSFFHFDDIVRVSKHVNEVLSGIKKIQVGIARLCTPKIDKYRWCRYIFQINSIKDISESNFKSHRSSISQGESYSQNFVRLSTTLNKNNIDFQDDILNCWLEASFSLCHYFNEYQPDFASISNGDLTNLDKKYKNLDCNKPSSVEQVQQFLQKDSPINLFEESSWYNVSTTPIGRSADLNYNKISLQTNENNFPYYHEQDSILLKELMRPVQNYC 1771
            MCKILSN+KKCELKKFQTKKVLTKKLTLSGLNDVVSRDFNSCFSNFPDITKNSDNNSTNKFSNCSEENALCKREKSRIAAKKRRTQENENLSELYISLPVATQSKLLSCYDNQFIGNFEKATIIKISAFVLNLYNSLSDLLFDNS QFDKHPLVSLFSQSIFGLFWCDKTSKLIFSSPGLARLTGLYWCNMIGMPRSSIIVECGSQDSSNMIFLRFMMSALKDRGIFTVNPQKPGNYKQSEESTLEKSQILYNQAKRDFIGKLPCSVWCSQKLPNGITFDLIQPLMIKSESFSHGTKFNQSHKHCPYRLPIFTDMHLREIKRPGHRFTSYHYLDSLCTMSVSGDFYSSLGYLKEDFSKSNLLSFFHFDDIVRVSKHVNEVLSGIKKIQVGIARLCTPKIDKYRWCRYIFQINSIKDISESNFKSHRSSISQGESYSQNFVRLSTTLNKNNIDFQDDILNCWLEASFSLCHYFNEYQPDFASISNGDLTNLDKKYKNLDCNKPSSVEQVQQFLQKDSPINLFEESSWYNVSTTPIGRSADLNYNKISLQTNENNFPYYHEQDSILLKELMRPVQNYC
Sbjct:    1 MCKILSNDKKCELKKFQTKKVLTKKLTLSGLNDVVSRDFNSCFSNFPDITKNSDNNSTNKFSNCSEENALCKREKSRIAAKKRRTQENENLSELYISLPVATQSKLLSCYDNQFIGNFEKATIIKISAFVLNLYNSLSDLLFDNSLQFDKHPLVSLFSQSIFGLFWCDKTSKLIFSSPGLARLTGLYWCNMIGMPRSSIIVECGSQDSSNMIFLRFMMSALKDRGIFTVNPQKPGNYKQSEESTLEKSQILYNQAKRDFIGKLPCSVWCSQKLPNGITFDLIQPLMIKSESFSHGTKFNQSHKHCPYRLPIFTDMHLREIKRPGHRFTSYHYLDSLCTMSVSGDFYSSLGYLKEDFSKSNLLSFFHFDDIVRVSKHVNEVLSGIKKIQVGIARLCTPKIDKYRWCRYIFQINSIKDISESNFKSHRSSISQGESYSQNFVRLSTTLNKNNIDFQDDILNCWLEASFSLCHYFNEYQPDFASISNGDLTNLDKKYKNLDCNKPSSVEQVQQFLQKDSPINLFEESSWYNVSTTPIGRSADLNYNKISLQTNENNFPYYHEQDSILLKELMRPVQNYC 576
[Close]
BLAST of SMED30027628 vs. Planmine SMEST
Match: SMESG000022424.1 (SMESG000022424.1)

HSP 1 Score: 52.7582 bits (125), Expect = 7.528e-7
Identity = 41/130 (31.54%), Postives = 69/130 (53.08%), Query Frame = 2 
Query:  239 EENALCKREKSRIAAKKRRTQENENLSELYISLPVATQSKLLSCYDNQFIGNFEKATIIKISAFVLNLYNSLSDLLFDNSFQFDKHPLVSLFSQSIFGLFWCDKTSKLIFSSPGLAR-LTGLYWCNMIGM 625
            E+ A  ++EKSR+AA+ RR +E++ L  L  +LP+   S       NQ +   EK+ +I+I+   L L  SL  +L      +   PL+S    S F  F+ D   +++++SP +   L   +W N+IG+
Sbjct:   27 EKKADKRKEKSRVAAQIRRNKESQTLILLQNALPICLPSP------NQML---EKSIVIRIAGQTLFLLKSLDQVL--GMTDWLTVPLIS----SNFLAFFTDNIGQIVYASPNIQNVLEQHHWSNLIGI 141
[Close]
BLAST of SMED30027628 vs. Planmine SMEST
Match: SMESG000022424.1 (SMESG000022424.1)

HSP 1 Score: 52.7582 bits (125), Expect = 7.667e-7
Identity = 41/130 (31.54%), Postives = 69/130 (53.08%), Query Frame = 2 
Query:  239 EENALCKREKSRIAAKKRRTQENENLSELYISLPVATQSKLLSCYDNQFIGNFEKATIIKISAFVLNLYNSLSDLLFDNSFQFDKHPLVSLFSQSIFGLFWCDKTSKLIFSSPGLAR-LTGLYWCNMIGM 625
            E+ A  ++EKSR+AA+ RR +E++ L  L  +LP+   S       NQ +   EK+ +I+I+   L L  SL  +L      +   PL+S    S F  F+ D   +++++SP +   L   +W N+IG+
Sbjct:   16 EKKADKRKEKSRVAAQIRRNKESQTLILLQNALPICLPSP------NQML---EKSIVIRIAGQTLFLLKSLDQVL--GMTDWLTVPLIS----SNFLAFFTDNIGQIVYASPNIQNVLEQHHWSNLIGI 130
The following BLAST results are available for this feature:
BLAST of SMED30027628 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027628 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027628 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027628 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027628 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027628 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027628 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027628 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3P6HMB27.995e-1336.30Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A5K3EMQ11.091e-1236.30Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A3P7D7A11.561e-1237.86Uncharacterized protein OS=Schistocephalus solidus... [more]
A0A183TDH31.602e-1237.86Uncharacterized protein OS=Schistocephalus solidus... [more]
A0A0V0J5962.985e-1237.86Uncharacterized protein (Fragment) OS=Schistocepha... [more]
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BLAST of SMED30027628 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027628 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027628 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027628 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027628 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027628 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000077694.10.000e+099.65SMESG000077694.1[more]
SMESG000022424.17.528e-731.54SMESG000022424.1[more]
SMESG000022424.17.667e-731.54SMESG000022424.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027628 ID=SMED30027628|Name=SMED30027628|organism=Schmidtea mediterranea sexual|type=transcript|length=1882bp
ATAATCACTTAATTAATAAAATTTGTAAAAATCTTAATTCAAAATGTGTA
AAATATTATCTAACAATAAAAAATGTGAACTTAAGAAATTTCAAACTAAA
AAAGTGTTGACTAAAAAGTTAACATTATCAGGCCTGAATGATGTAGTATC
ACGCGATTTTAATAGTTGTTTTAGTAATTTTCCAGATATAACTAAAAACT
CTGATAATAATTCTACAAATAAATTTTCTAATTGCTCTGAAGAAAATGCA
TTGTGCAAACGAGAAAAATCTCGAATAGCAGCAAAAAAGCGTCGAACTCA
AGAAAATGAAAATCTTAGTGAATTATATATATCTTTACCTGTTGCAACTC
AATCAAAACTTCTCTCTTGTTATGATAACCAGTTTATTGGGAATTTTGAA
AAGGCGACTATTATCAAAATATCAGCATTTGTACTTAATCTATACAATTC
TTTATCAGATCTTCTATTCGACAATTCTTTCCAGTTTGACAAACACCCAT
TAGTTTCATTATTCAGTCAATCAATATTCGGATTGTTTTGGTGTGACAAA
ACTTCAAAACTAATATTTTCTTCACCTGGCCTAGCTAGGTTAACAGGATT
GTATTGGTGTAATATGATTGGAATGCCTAGAAGTAGTATTATTGTTGAAT
GTGGAAGTCAAGATTCATCAAATATGATTTTTTTACGTTTTATGATGAGC
GCCTTAAAAGATAGAGGAATTTTCACAGTAAATCCTCAAAAACCTGGAAA
TTATAAGCAATCAGAAGAATCCACGTTAGAAAAGTCTCAAATTTTATATA
ATCAAGCTAAAAGAGATTTCATAGGAAAATTGCCTTGTTCTGTGTGGTGT
TCTCAAAAATTGCCTAATGGCATTACTTTTGACCTAATCCAACCTTTAAT
GATAAAATCCGAGAGCTTTTCTCATGGAACCAAATTTAATCAATCACACA
AGCACTGCCCTTATAGACTACCAATTTTCACTGATATGCACCTTAGAGAA
ATCAAACGTCCAGGTCATAGATTCACCAGTTATCATTATTTAGATTCATT
ATGTACAATGTCAGTTAGTGGAGATTTCTATAGTTCTCTTGGATATTTGA
AAGAAGATTTTTCCAAATCAAATCTCTTAAGCTTTTTTCATTTTGATGAT
ATTGTTCGAGTATCTAAACATGTAAATGAAGTTCTTTCTGGAATAAAAAA
AATACAAGTTGGTATTGCTAGGTTATGCACTCCAAAAATCGATAAATATC
GTTGGTGCCGTTATATTTTTCAAATTAATAGCATAAAAGACATCTCTGAA
TCAAATTTCAAAAGTCACAGATCTAGTATCAGTCAAGGAGAATCGTATTC
TCAAAATTTTGTTCGATTATCAACTACATTAAACAAGAATAATATAGATT
TTCAAGATGATATTCTTAATTGTTGGCTTGAAGCTTCTTTCTCACTGTGC
CATTATTTTAATGAATACCAACCAGATTTTGCCTCAATATCAAATGGTGA
TTTGACCAATTTAGATAAGAAATATAAAAACTTAGATTGTAATAAACCAT
CCTCAGTGGAACAAGTTCAACAATTTCTTCAAAAAGACAGCCCAATAAAT
TTATTTGAGGAATCGAGTTGGTATAATGTTTCAACAACTCCAATTGGACG
TTCTGCTGATCTGAATTATAATAAAATATCACTTCAAACTAATGAAAATA
ATTTTCCATACTACCATGAGCAAGATTCAATACTTTTGAAAGAATTAATG
AGGCCGGTTCAAAATTATTGTTAACAAAAAGAATAAACTTGTAACATGTC
TATAAATATAATTTAATGAAAATAATATTTTTCTATTTATTTATGAAAGT
GTTCATTTAAAATATTTTTTCATAGAATAAAA
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protein sequence of SMED30027628-orf-1

>SMED30027628-orf-1 ID=SMED30027628-orf-1|Name=SMED30027628-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=577bp
MCKILSNNKKCELKKFQTKKVLTKKLTLSGLNDVVSRDFNSCFSNFPDIT
KNSDNNSTNKFSNCSEENALCKREKSRIAAKKRRTQENENLSELYISLPV
ATQSKLLSCYDNQFIGNFEKATIIKISAFVLNLYNSLSDLLFDNSFQFDK
HPLVSLFSQSIFGLFWCDKTSKLIFSSPGLARLTGLYWCNMIGMPRSSII
VECGSQDSSNMIFLRFMMSALKDRGIFTVNPQKPGNYKQSEESTLEKSQI
LYNQAKRDFIGKLPCSVWCSQKLPNGITFDLIQPLMIKSESFSHGTKFNQ
SHKHCPYRLPIFTDMHLREIKRPGHRFTSYHYLDSLCTMSVSGDFYSSLG
YLKEDFSKSNLLSFFHFDDIVRVSKHVNEVLSGIKKIQVGIARLCTPKID
KYRWCRYIFQINSIKDISESNFKSHRSSISQGESYSQNFVRLSTTLNKNN
IDFQDDILNCWLEASFSLCHYFNEYQPDFASISNGDLTNLDKKYKNLDCN
KPSSVEQVQQFLQKDSPINLFEESSWYNVSTTPIGRSADLNYNKISLQTN
ENNFPYYHEQDSILLKELMRPVQNYC*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000101muscle cell
PLANA:0002032epidermal cell
PLANA:0002056medial muscle cell
PLANA:0003001lateral region of the whole animal
PLANA:0003116parenchymal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.30.450.20coord: 316..464
e-value: 7.1E-7
score: 30.8