SMED30027622

Overview
NameSMED30027622
Smed IDSMED30027622
Length (bp)2703
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30027622 (SMED30027622) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30027622 (SMED30027622) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30027622 (SMED30027622) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30027622 (SMED30027622) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30027622

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30027622SMESG000032521.1 SMESG000032510.1 SMESG000022393.1 SmedASXL_014243SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
cephalic gangliaSMED30027622SMESG000032521.1 SMESG000032510.1 SMESG000022393.1 dd_Smed_v4_10555_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30027622SMESG000032521.1 SMESG000032510.1 SMESG000022393.1 dd_Smed_v4_10555_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30027622SMESG000032521.1 SMESG000032510.1 SMESG000022393.1 dd_Smed_v4_10555_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027622SMESG000032521.1 SMESG000032510.1 SMESG000022395.1 SMESG000022393.1 Contig42235newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027622SMESG000032521.1 SMESG000032510.1 SMESG000022395.1 SMESG000022393.1 Contig42235uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30027622SMESG000032521.1 SMESG000032510.1 SMESG000022393.1 dd_Smed_v6_10555_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30027622SMESG000032521.1 SMESG000032510.1 SMESG000022393.1 dd_Smed_v4_10555_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of SMED30027622 vs. Planmine SMEST
Match: SMESG000022393.1 (SMESG000022393.1)

HSP 1 Score: 1305.43 bits (3377), Expect = 0.000e+0
Identity = 744/754 (98.67%), Postives = 747/754 (99.07%), Query Frame = 2 
Query:  224 MALDSPVISDQDSLIEQAPMQTGDQISRGSTNYSTFTRKRPVNLPNSLPRKKRQSLMSPQSIXXXXXXXXXXXXFAMTQSMYEPLEKRKQQAKLEANSKLLNLLASEKISQAAKERKRKEIKLKAMXXXXXXXXXXXXXXXRRLRMLADHEAKKKRIIEKINLPSPMTASCINTMNRSVLKQRASMGSGFIEKRPISSTGKVFGFGSSATRDVCEGYTLKKSRDKDTFYVDHKKHEQNVSRMCTSQIISHYNENKMYKSSKLPKENSVKPISVSTYNISNFNKSLIKSKKVSSGNRKDNESGSRINLFIPAIHEVKPKQIHTNINGISKNASIPNQIKSDPKQKSRGRSADIEKSNSNIPVHKTPRSRTTERTKPPPKHVTLQTNSAIPNQKPIQKFESVKIIQXXXXXXXXXXXXQNENEIKTKMSDNSNLLQPEVTRRPEVRFEEKENIKDSDCNLLXXXXXXXXXXXXXXXXLTEEEYKQRISHXXXXXXXXXXXXXXXXXXXLLKEQNEMQTLQQIQKSEIVSCDIKDKTELQISTENNISIDESESTQSSKRETEEDSLPKIITNEINKTEVEPLDIHTVSTVDTIPIINIPVEIPSPSIEMTTSTEMLDRIERKKRLDSIMSRIKKPIGGSTENVSLHMSSTTLQDMLNKGHLSGMSKTAAILMRTTHASQDLSASSVELHTSEESKILNPSCDDNLGPADTPILKSEPEIEISQESRGSCDGQEDEISPSRFEENLTTKSNLMENLV 2485
            MALDSPVISDQDSLIEQAPMQTGDQISRGSTNYSTFTRKRPVNLPNSLPRKKRQSLMSPQSIPTLDSDDLLTPDFAMTQSMYEPLEKRKQQAKLEANSKLLNLLASEKISQAAKERKRKEIKLKAMEQKKKNEEKQKQAEERRLRMLADHEAKKKRIIEKINLPSPMTASCINTMNRSVLKQRASMGSGFIEKRPISSTGKVFGFGSSATRDVCEGYTLKKSRDKDTFYVDHKKHEQNVSRMCTSQIISH NENKMYKSSKLPKENSVK ISVSTYNISNFNKSLIKSKKVSSGNRKDNESGSRI LFIPAIHEVKPKQIHTNINGISKNASIPNQIKSDPKQKSRGRSADIEKSNSNIPVHKTPRSRTTERTKPP KHVTLQTNSAIPNQKPIQKFESVKIIQSHHKSASSLKHAQNENEIKTKMSDNSNLLQPEVTRRPEVRFEEKENIKDSD NLLSDSSISVDRRRISNNNLTEEEYKQRISHQRKLAKEKRRKEQEEKDQRLLKEQNE+QTLQQIQKSEIVSCDIKDKTELQISTENNISIDESESTQSSKRETEEDSLPKIITNEINKTEVEPLDIHTVSTVDTIP+INIPVEI SPSIEMTTSTEMLDRIERKKRLDSIMSRIKKPIGGSTENVSLHMSSTTLQDMLNKGHLSGMSKTAAILMRTTHASQDLSASSVELHTSEESKILNPSCDD+LGPADTPILKSEPEIEISQESRGSCDGQEDEISPSRFEENLTTKSNLMENL 
Sbjct:    1 MALDSPVISDQDSLIEQAPMQTGDQISRGSTNYSTFTRKRPVNLPNSLPRKKRQSLMSPQSIPTLDSDDLLTPDFAMTQSMYEPLEKRKQQAKLEANSKLLNLLASEKISQAAKERKRKEIKLKAMEQKKKNEEKQKQAEERRLRMLADHEAKKKRIIEKINLPSPMTASCINTMNRSVLKQRASMGSGFIEKRPISSTGKVFGFGSSATRDVCEGYTLKKSRDKDTFYVDHKKHEQNVSRMCTSQIISHSNENKMYKSSKLPKENSVKLISVSTYNISNFNKSLIKSKKVSSGNRKDNESGSRIILFIPAIHEVKPKQIHTNINGISKNASIPNQIKSDPKQKSRGRSADIEKSNSNIPVHKTPRSRTTERTKPPSKHVTLQTNSAIPNQKPIQKFESVKIIQSHHKSASSLKHAQNENEIKTKMSDNSNLLQPEVTRRPEVRFEEKENIKDSDSNLLSDSSISVDRRRISNNNLTEEEYKQRISHQRKLAKEKRRKEQEEKDQRLLKEQNEIQTLQQIQKSEIVSCDIKDKTELQISTENNISIDESESTQSSKRETEEDSLPKIITNEINKTEVEPLDIHTVSTVDTIPVINIPVEISSPSIEMTTSTEMLDRIERKKRLDSIMSRIKKPIGGSTENVSLHMSSTTLQDMLNKGHLSGMSKTAAILMRTTHASQDLSASSVELHTSEESKILNPSCDDSLGPADTPILKSEPEIEISQESRGSCDGQEDEISPSRFEENLTTKSNLMENLT 754
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BLAST of SMED30027622 vs. Planmine SMEST
Match: SMESG000032510.1 (SMESG000032510.1)

HSP 1 Score: 867.07 bits (2239), Expect = 0.000e+0
Identity = 530/546 (97.07%), Postives = 532/546 (97.44%), Query Frame = 2 
Query:  392 MSPQSIXXXXXXXXXXXXFAMTQSMYEPLEKRKQQAKLEANSKLLNLLASEKISQAAKERKRKEIKLKAMXXXXXXXXXXXXXXXRRLRMLADHEAKKKRIIEKINLPSPMTASCINTMNRSVLKQRASMGSGFIEKRPISSTGKVFGFGSSATRDVCEGYTLKKSRDKDTFYVDHKKHEQNVSRMCTSQIISHYNENKMYKSSKLPKENSVKPISVSTYNISNFNKSLIKSKKVSSGNRKDNESGSRINLFIPAIHEVKPKQIHTNINGISKNASIPNQIKSDPKQKSRGRSADIEKSNSNIPVHKTPRSRTTERTKPPPKHVTLQTNSAIPNQKPIQKFESVKIIQXXXXXXXXXXXXQNENEIKTKMSDNSNLLQPEVTRRPEVRFEEKENIKDSDCNLLXXXXXXXXXXXXXXXXLTEEEYKQRISHXXXXXXXXXXXXXXXXXXXLLKEQNEMQTLQQIQKSEIVSCDIKDKTELQISTENNISIDESESTQSSKRETEEDSLPKIITNEINKTEVEPLDIHTVSTVDTIPIINIPVEIPS 2029
            MSPQSIPTLDSDDLLTPDFAMTQSMYEPLEKRKQQAKLEANSKLLNLLASEKISQAAKERKRKEIKLKAMEQKKKNEEKQKQAEERRLRMLADHEAKKKRIIEKINLPSPMTASCINTMNRSVLKQRASMGSGFIEKRPISSTGKVFGFGSSATRDVCEGYTLKKSRDKDTFYVDHKKHEQNVSRMCTSQIISH NENK          NSVK ISVSTYNISNFNKSLIKSKKVSSGNRKDNESGSRINLFIPAIHEVKPKQIHTNINGISKNASIPNQIKSDPKQKSRGRSADIEKSNSNIPVHKTPRSRTTERTKPPPKHVTLQTNSAIPNQKPIQKFESVKIIQSHHKSASSLKHAQNENEIKTKMSDNSNLLQPEVTRRPEVRFEEKENIKDSD NLLSDSSISVDRRRISNNNLTEEEYKQRISHQRKLAKEKRRKEQEEKDQRLLKEQNEMQTLQQIQKSEIVSCDIKDKTELQISTENNISIDESESTQSSKRETEEDSLPKIITNEINKTEVEPLDIHTVSTVDTIP+INIPVE+ S
Sbjct:    1 MSPQSIPTLDSDDLLTPDFAMTQSMYEPLEKRKQQAKLEANSKLLNLLASEKISQAAKERKRKEIKLKAMEQKKKNEEKQKQAEERRLRMLADHEAKKKRIIEKINLPSPMTASCINTMNRSVLKQRASMGSGFIEKRPISSTGKVFGFGSSATRDVCEGYTLKKSRDKDTFYVDHKKHEQNVSRMCTSQIISHSNENK----------NSVKLISVSTYNISNFNKSLIKSKKVSSGNRKDNESGSRINLFIPAIHEVKPKQIHTNINGISKNASIPNQIKSDPKQKSRGRSADIEKSNSNIPVHKTPRSRTTERTKPPPKHVTLQTNSAIPNQKPIQKFESVKIIQSHHKSASSLKHAQNENEIKTKMSDNSNLLQPEVTRRPEVRFEEKENIKDSDSNLLSDSSISVDRRRISNNNLTEEEYKQRISHQRKLAKEKRRKEQEEKDQRLLKEQNEMQTLQQIQKSEIVSCDIKDKTELQISTENNISIDESESTQSSKRETEEDSLPKIITNEINKTEVEPLDIHTVSTVDTIPVINIPVEVIS 536
[Close]
BLAST of SMED30027622 vs. Planmine SMEST
Match: SMESG000022393.1 (SMESG000022393.1)

HSP 1 Score: 295.049 bits (754), Expect = 1.119e-94
Identity = 146/148 (98.65%), Postives = 147/148 (99.32%), Query Frame = 2 
Query: 2042 MTTSTEMLDRIERKKRLDSIMSRIKKPIGGSTENVSLHMSSTTLQDMLNKGHLSGMSKTAAILMRTTHASQDLSASSVELHTSEESKILNPSCDDNLGPADTPILKSEPEIEISQESRGSCDGQEDEISPSRFEENLTTKSNLMENLV 2485
            MTTSTEMLDRIERKKRLDSIMSRIKKPIGGSTENVSLHMSSTTLQDMLNKGHLSGMSKTAAILMRTTHASQDLSASSVELHTSEESKILNPSCDD+LGPADTPILKSEPEIEISQESRGSCDGQEDEISPSRFEENLTTKSNLMENL 
Sbjct:    1 MTTSTEMLDRIERKKRLDSIMSRIKKPIGGSTENVSLHMSSTTLQDMLNKGHLSGMSKTAAILMRTTHASQDLSASSVELHTSEESKILNPSCDDSLGPADTPILKSEPEIEISQESRGSCDGQEDEISPSRFEENLTTKSNLMENLT 148
[Close]
BLAST of SMED30027622 vs. Planmine SMEST
Match: SMESG000022393.1 (SMESG000022393.1)

HSP 1 Score: 284.263 bits (726), Expect = 1.220e-90
Identity = 140/142 (98.59%), Postives = 141/142 (99.30%), Query Frame = 2 
Query: 2060 MLDRIERKKRLDSIMSRIKKPIGGSTENVSLHMSSTTLQDMLNKGHLSGMSKTAAILMRTTHASQDLSASSVELHTSEESKILNPSCDDNLGPADTPILKSEPEIEISQESRGSCDGQEDEISPSRFEENLTTKSNLMENLV 2485
            MLDRIERKKRLDSIMSRIKKPIGGSTENVSLHMSSTTLQDMLNKGHLSGMSKTAAILMRTTHASQDLSASSVELHTSEESKILNPSCDD+LGPADTPILKSEPEIEISQESRGSCDGQEDEISPSRFEENLTTKSNLMENL 
Sbjct:    1 MLDRIERKKRLDSIMSRIKKPIGGSTENVSLHMSSTTLQDMLNKGHLSGMSKTAAILMRTTHASQDLSASSVELHTSEESKILNPSCDDSLGPADTPILKSEPEIEISQESRGSCDGQEDEISPSRFEENLTTKSNLMENLT 142
[Close]
BLAST of SMED30027622 vs. Planmine SMEST
Match: SMESG000032521.1 (SMESG000032521.1)

HSP 1 Score: 221.476 bits (563), Expect = 1.087e-67
Identity = 109/110 (99.09%), Postives = 109/110 (99.09%), Query Frame = 2 
Query: 2156 MSSTTLQDMLNKGHLSGMSKTAAILMRTTHASQDLSASSVELHTSEESKILNPSCDDNLGPADTPILKSEPEIEISQESRGSCDGQEDEISPSRFEENLTTKSNLMENLV 2485
            MSSTTLQDMLNKGHLSGMSKTAAILMRTTHASQDLSASSVELHTSEESKILNPSCDDNLGPADTPILKSEPEIEISQESRGSCDGQEDEISPSRFEENLTTKSNLMENL 
Sbjct:    1 MSSTTLQDMLNKGHLSGMSKTAAILMRTTHASQDLSASSVELHTSEESKILNPSCDDNLGPADTPILKSEPEIEISQESRGSCDGQEDEISPSRFEENLTTKSNLMENLT 110
The following BLAST results are available for this feature:
BLAST of SMED30027622 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027622 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027622 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027622 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027622 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027622 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027622 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027622 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027622 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027622 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027622 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027622 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027622 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027622 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000022393.10.000e+098.67SMESG000022393.1[more]
SMESG000032510.10.000e+097.07SMESG000032510.1[more]
SMESG000022393.11.119e-9498.65SMESG000022393.1[more]
SMESG000022393.11.220e-9098.59SMESG000022393.1[more]
SMESG000032521.11.087e-6799.09SMESG000032521.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027622 ID=SMED30027622|Name=SMED30027622|organism=Schmidtea mediterranea sexual|type=transcript|length=2703bp
CCGGGTTGTTTCTTCTGACAACGCATGTGTCTTTCAATAATCAACTCTCA
TTCCATTGAAACAGTTCCGTAACCTTTCTCTGTTTCGATTATTATTCCAT
TTTGAATTTTTCAAATTTCGCCAAGGAGACTTTGCCGACAAAATTATTCA
CTGATTTTATTTCACCTTTCGAGCGTTAAACTTTCTCTTTCAAAACGCCT
TTCAATTTTGGAAAAATTCTACAATGGCTTTAGACTCTCCAGTAATATCT
GATCAGGATTCTCTAATCGAACAAGCTCCTATGCAAACTGGGGACCAGAT
TTCTCGAGGTTCTACGAATTATTCAACGTTTACAAGAAAAAGACCTGTAA
ATCTACCAAATTCTCTTCCAAGGAAAAAACGCCAGTCACTGATGAGCCCA
CAGTCTATTCCAACGTTGGACTCTGACGATTTATTAACTCCGGATTTCGC
AATGACACAATCAATGTATGAGCCGTTAGAAAAACGAAAGCAACAAGCAA
AACTGGAAGCTAATTCTAAATTACTGAATTTATTAGCGTCAGAAAAAATA
TCCCAAGCAGCCAAAGAAAGAAAGCGAAAAGAAATAAAATTAAAAGCTAT
GGAACAAAAGAAAAAAAATGAAGAAAAGCAAAAACAAGCGGAAGAAAGGA
GACTTCGAATGCTTGCAGACCACGAAGCCAAAAAGAAGAGAATAATAGAA
AAGATAAATCTTCCAAGCCCAATGACAGCGAGTTGCATTAATACAATGAA
TAGAAGTGTGTTGAAGCAAAGAGCTTCCATGGGTAGTGGGTTTATAGAAA
AAAGACCAATTTCGTCTACTGGTAAAGTTTTCGGATTTGGATCAAGTGCA
ACTAGAGACGTTTGTGAAGGATACACTCTTAAAAAGTCTAGAGACAAGGA
TACATTTTATGTTGATCATAAGAAACATGAACAAAACGTATCAAGAATGT
GTACCAGTCAAATTATAAGTCATTACAATGAGAATAAAATGTATAAATCT
AGTAAATTGCCAAAAGAAAATTCAGTGAAACCGATTTCAGTGTCAACTTA
TAATATTTCGAATTTCAACAAATCCCTCATCAAAAGCAAAAAAGTCTCCA
GTGGTAACAGAAAAGATAATGAGAGTGGAAGCAGAATAAATCTATTTATA
CCGGCAATCCATGAAGTGAAACCCAAACAAATCCACACTAATATCAATGG
GATATCAAAAAATGCTTCCATTCCAAATCAAATCAAAAGTGATCCAAAAC
AGAAATCACGTGGTAGATCAGCAGATATTGAGAAATCTAATTCAAATATT
CCGGTCCATAAAACCCCAAGATCAAGAACTACTGAAAGAACGAAACCTCC
TCCAAAACACGTGACACTCCAAACCAACAGTGCAATTCCTAATCAAAAAC
CAATTCAAAAATTTGAAAGTGTGAAAATTATTCAATCTCATCACAAATCG
GCATCATCACTCAAACACGCACAAAATGAAAATGAAATTAAAACAAAAAT
GTCAGATAATTCTAACCTGCTTCAACCAGAGGTTACGAGACGGCCAGAAG
TTCGATTTGAAGAAAAAGAAAATATTAAGGATTCTGATTGTAATTTACTC
TCCGATTCTTCGATCTCCGTTGACCGTCGACGAATATCAAATAACAACTT
GACAGAAGAAGAATACAAACAACGAATTTCTCATCAGAGAAAATTAGCCA
AAGAAAAAAGAAGAAAAGAACAAGAAGAAAAAGATCAAAGACTATTAAAA
GAACAAAATGAAATGCAAACCTTACAACAAATACAAAAATCGGAAATTGT
GAGTTGTGATATAAAAGATAAAACTGAATTACAAATATCAACTGAAAACA
ATATTTCCATTGATGAGAGCGAATCAACGCAGTCGTCGAAGCGCGAAACG
GAGGAAGATAGTTTGCCGAAGATTATAACAAACGAAATAAATAAAACAGA
AGTGGAGCCCCTTGACATTCATACCGTTTCAACTGTTGACACTATCCCTA
TAATAAACATTCCAGTAGAAATTCCAAGTCCAAGCATAGAAATGACTACC
TCTACAGAAATGCTCGACCGTATTGAAAGAAAGAAAAGGCTAGACTCGAT
AATGAGTAGAATTAAAAAACCGATTGGAGGTTCCACAGAAAATGTCAGCC
TTCACATGAGTTCAACGACTCTTCAAGACATGCTAAATAAGGGTCATTTG
AGTGGAATGTCAAAAACCGCTGCAATTTTAATGAGAACTACTCATGCATC
ACAAGATCTATCAGCTTCTTCAGTCGAACTTCATACTTCCGAAGAAAGTA
AAATCCTAAATCCATCATGTGACGATAATCTGGGTCCTGCTGATACACCA
ATTTTAAAATCTGAGCCGGAAATTGAAATTTCACAAGAATCAAGGGGATC
CTGTGATGGACAGGAAGATGAAATTAGCCCAAGTAGATTCGAAGAGAATC
TAACAACAAAGTCGAATTTGATGGAAAATCTAGTTTCCAATTCTAATAAG
CCTTATTTTTCATCGTGGCAAGAAAAATACTCTGACAGTTGTTCCCTAGA
CGCAGATGATGATAATACATGATAATTATCCCGAAAGATCAACGAGAAAA
TTCTGATCAGATCATTTATGACATTATTTATGGTGATGAGGTTTTTATGA
TGAATGTTGTAATAATTGGAATTTTGTTGCAGATTAATAGTGGAAATGTA
CAA
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protein sequence of SMED30027622-orf-1

>SMED30027622-orf-1 ID=SMED30027622-orf-1|Name=SMED30027622-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=783bp
MALDSPVISDQDSLIEQAPMQTGDQISRGSTNYSTFTRKRPVNLPNSLPR
KKRQSLMSPQSIPTLDSDDLLTPDFAMTQSMYEPLEKRKQQAKLEANSKL
LNLLASEKISQAAKERKRKEIKLKAMEQKKKNEEKQKQAEERRLRMLADH
EAKKKRIIEKINLPSPMTASCINTMNRSVLKQRASMGSGFIEKRPISSTG
KVFGFGSSATRDVCEGYTLKKSRDKDTFYVDHKKHEQNVSRMCTSQIISH
YNENKMYKSSKLPKENSVKPISVSTYNISNFNKSLIKSKKVSSGNRKDNE
SGSRINLFIPAIHEVKPKQIHTNINGISKNASIPNQIKSDPKQKSRGRSA
DIEKSNSNIPVHKTPRSRTTERTKPPPKHVTLQTNSAIPNQKPIQKFESV
KIIQSHHKSASSLKHAQNENEIKTKMSDNSNLLQPEVTRRPEVRFEEKEN
IKDSDCNLLSDSSISVDRRRISNNNLTEEEYKQRISHQRKLAKEKRRKEQ
EEKDQRLLKEQNEMQTLQQIQKSEIVSCDIKDKTELQISTENNISIDESE
STQSSKRETEEDSLPKIITNEINKTEVEPLDIHTVSTVDTIPIINIPVEI
PSPSIEMTTSTEMLDRIERKKRLDSIMSRIKKPIGGSTENVSLHMSSTTL
QDMLNKGHLSGMSKTAAILMRTTHASQDLSASSVELHTSEESKILNPSCD
DNLGPADTPILKSEPEIEISQESRGSCDGQEDEISPSRFEENLTTKSNLM
ENLVSNSNKPYFSSWQEKYSDSCSLDADDDNT*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000418head
PLANA:0003117parapharyngeal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 482..520
NoneNo IPR availableCOILSCoilCoilcoord: 109..150
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..59
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 713..742
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 478..505
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 717..739
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 325..394
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 541..564
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 10..59
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 325..340
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 376..393