RNA-binding protein pno1

Overview


Name	RNA-binding protein pno1
Smed ID	SMED30027596
Length (bp)	660

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of RNA-binding protein pno1 (SMED30027596) t-SNE clustered cells

Violin plots show distribution of expression levels for RNA-binding protein pno1 (SMED30027596) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of RNA-binding protein pno1 (SMED30027596) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for RNA-binding protein pno1 (SMED30027596) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30027596

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 4

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
pharynx	SMED30027596	SMESG000015341.1	dd_Smed_v4_1077_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30027596	SMESG000015341.1	dd_Smed_v4_1077_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
gut	SMED30027596	SMESG000015341.1	dd_Smed_v4_1077_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30027596	SMESG000015341.1	dd_Smed_v4_1077_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Homology

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BLAST of RNA-binding protein pno1 vs. Ensembl Human
Match: PNO1 (partner of NOB1 homolog [Source:HGNC Symbol;Acc:HGNC:32790])

HSP 1 Score: 107.842 bits (268), Expect = 4.638e-28
Identity = 67/177 (37.85%), Postives = 97/177 (54.80%), Query Frame = 3

Query:   66 TRSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVRSPVNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKLGQV-KRSLD 593
            TR + VP NR  PL+E W+ I   +   LGLQ       R+  +       D   L K  +F+ AF  GF+  D  +LIR+D L++++  I D+ + +KGDH +RAIGRI G  G  +  +EN TR RIV++D   HI+GS+Q + +A   +  L  G   +KV G +  V  RS D
Sbjct:   75 TRKIPVPANRYTPLKENWMKIFTPIVEHLGLQIRFNLKSRNVEIRTCKETKDVSALTKAADFVKAFILGFQVEDALALIRLDDLFLESFEITDV-KPLKGDHLSRAIGRIAGKGGKTKFTIENVTRTRIVLADVKVHILGSFQNIKMARTAICNLILGNPPSKVYGNIRAVASRSAD 250

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BLAST of RNA-binding protein pno1 vs. Ensembl Celegans
Match: pno-1 (RNA-binding protein pno-1 [Source:UniProtKB/Swiss-Prot;Acc:O18216])

HSP 1 Score: 118.627 bits (296), Expect = 2.234e-32
Identity = 69/170 (40.59%), Postives = 104/170 (61.18%), Query Frame = 3

Query:   66 TRSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVRSPVN--DEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKL 569
            +R V VP +R  PL++ WV+I   +   LGLQ      KR   V +R+PV+  D   LQK  +F+ AF  GFE  D  +LIR+D L+++T  + D+   +KGDH +RAIGRI G +G  +  +EN T+ RIV++++  HI+G+YQ + +A   V +L  G+  +KV G L
Sbjct:   99 SRVVPVPKHRYTPLKDNWVNIFTPIVKNLGLQVRFNLKKRQ--VEIRNPVDREDTTDLQKATDFVRAFILGFEVNDAIALIRLDHLFLETFEVADVKHSLKGDHVSRAIGRIAGKDGRTKLVIENTTKTRIVVANTKIHILGAYQNLKLARNAVCSLILGSNPSKVYGSL 266

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Query:   66 TRSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVRSPVN--DEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKL 569
            +R V VP +R  PL++ WV+I   +   LGLQ      KR   V +R+PV+  D   LQK  +F+ AF  GFE  D  +LIR+D L+++T  + D+   +KGDH +RAIGRI G +G  +  +EN T+ RIV++++  HI+G+YQ + +A   V +L  G+  +KV G L
Sbjct:   99 SRVVPVPKHRYTPLKDNWVNIFTPIVKNLGLQVRFNLKKRQ--VEIRNPVDREDTTDLQKATDFVRAFILGFEVNDAIALIRLDHLFLETFEVADVKHSLKGDHVSRAIGRIAGKDGRTKLVIENTTKTRIVVANTKIHILGAYQNLKLARNAVCSLILGSNPSKVYGSL 266

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BLAST of RNA-binding protein pno1 vs. Ensembl Fly
Match: l(1)G0004 (gene:FBgn0027334 transcript:FBtr0077298)

HSP 1 Score: 113.235 bits (282), Expect = 1.276e-30
Identity = 66/174 (37.93%), Postives = 99/174 (56.90%), Query Frame = 3

Query:   69 RSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVRSPVNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKLGQVKRSL 590
            R V+VP +R   L+E W+ I   +   + LQ       R   + V     D   LQ+G +F+ AF  GFE  D  +L+R++ L+V++  IKD+ + ++GDH +RAIGR+ G  G  +  +EN T+ RIV++DS  HI+GSYQ + +A R V  L  G+  +KV G L  V   L
Sbjct:   64 RKVSVPPHRYSSLKEHWMKIFTPVVEHMKLQIRFNMKARQVELRVGPETPDIANLQRGADFVRAFLCGFEVDDALALLRLEDLFVESFEIKDV-KTLRGDHQSRAIGRLAGKGGRTKFTIENVTKTRIVLADSKIHILGSYQNIQLARRAVCNLILGSPPSKVYGNLRAVASRL 236

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BLAST of RNA-binding protein pno1 vs. Ensembl Zebrafish
Match: pno1 (partner of NOB1 homolog [Source:NCBI gene;Acc:393520])

HSP 1 Score: 105.145 bits (261), Expect = 3.608e-27
Identity = 60/167 (35.93%), Postives = 92/167 (55.09%), Query Frame = 3

Query:   69 RSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVRSPVNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKL 569
            R V VP +R  PL+E W+ I   +   L LQ       R   +       D   L +  +F+ AF  GF+  D  +LIR+D L+++T  + D+ + +KGDH +RAIGRI G  G  +  +EN T+ RIV++D+  HI+GS+Q + +A   +  L  G+  +KV G L
Sbjct:   76 RKVPVPSHRYTPLKENWLKIFTPIVENLQLQVRFNLKTRQVEIKTCKETQDIGALTRAADFVKAFVLGFQVEDALALIRLDELFLETFDVTDV-KPLKGDHLSRAIGRIAGKGGKTKFTIENVTKTRIVLADTKIHILGSFQNIKMARTAICNLILGSPPSKVYGNL 241

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BLAST of RNA-binding protein pno1 vs. Ensembl Xenopus
Match: ehf (ETS homologous factor [Source:Xenbase;Acc:XB-GENE-853705])

HSP 1 Score: 105.916 bits (263), Expect = 1.992e-27
Identity = 61/170 (35.88%), Postives = 94/170 (55.29%), Query Frame = 3

Query:   69 RSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVRSPVNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKLGQV 578
            R V VP +R  PL+E W+ I   +   L LQ       R+  +       D   L K  +F+ AF  GF+  D  +L+R+D L++++  + D+ + +KGDH +RAIGRI G  G  +  +EN TR RIV++DS  HI+GS+Q + +A   +  L  G+  +KV G +  V
Sbjct:   62 RKVPVPSHRYTPLKENWMKIFTPIVEHLQLQVRFNLKTRNVEIKTCKETTDVGALTKATDFVRAFILGFQVEDALALVRLDDLFLESFEVTDV-KPLKGDHLSRAIGRIAGKGGKTKFTIENVTRTRIVLADSKIHILGSFQNIKMARTAICNLILGSPPSKVYGNIRAV 230

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BLAST of RNA-binding protein pno1 vs. Ensembl Mouse
Match: Pno1 (partner of NOB1 homolog [Source:MGI Symbol;Acc:MGI:1913499])

HSP 1 Score: 106.686 bits (265), Expect = 9.750e-28
Identity = 67/177 (37.85%), Postives = 97/177 (54.80%), Query Frame = 3

Query:   66 TRSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVRSPVNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKLGQV-KRSLD 593
            TR + VP NR  PL+E W+ I   +   LGLQ       R+  +       D   L K  +F+ AF  GF+  D  +LIR+D L++++  I D+ + +KGDH +RAIGRI G  G  +  +EN TR RIV++D   HI+GS+Q + +A   +  L  G   +KV G +  V  RS D
Sbjct:   71 TRKIPVPGNRYTPLKENWMKIFTPIVEHLGLQIRFNLKSRNVEIRTCKDTKDVSALTKAADFVKAFVLGFQVEDALALIRLDDLFLESFEITDV-KPLKGDHLSRAIGRIAGKGGKTKFTIENVTRTRIVLADVHVHILGSFQNIKMARTAICNLILGNPPSKVYGNIRAVASRSAD 246

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BLAST of RNA-binding protein pno1 vs. UniProt/SwissProt
Match: sp|Q5B0Z5|PNO1_EMENI (Pre-rRNA-processing protein pno1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=pno1 PE=3 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 7.435e-32
Identity = 67/179 (37.43%), Postives = 97/179 (54.19%), Query Frame = 3

Query:   66 TRSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVRSPVNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKLGQVKRSLDSTF 602
            TR V VP +R+ PL+  W  I   L   L LQ       R+  +       D   LQKG +F+ AF  GF+  D  +L+R+D LY+++  I+D+   + G+H +RAIGRI G +G  R  +ENA+R RIVI+D   HI+G +Q ++     V +L  GA   KV G L +V   +   F
Sbjct:   80 TRKVPVPPHRMTPLKANWPKIYPPLVEHLKLQVRINIKSRAVELRTSKFTTDTGALQKGEDFVKAFTLGFDIDDAIALLRLDDLYIRSFEIRDVKASLNGEHLSRAIGRIAGKDGRTRHAIENASRTRIVIADQKIHILGRFQNINAGQEAVVSLILGAPPGKVYGNLRKVAARMKERF 258

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BLAST of RNA-binding protein pno1 vs. UniProt/SwissProt
Match: sp|Q2UMG1|PNO1_ASPOR (Pre-rRNA-processing protein pno1 OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=pno1 PE=3 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 1.443e-31
Identity = 66/179 (36.87%), Postives = 98/179 (54.75%), Query Frame = 3

Query:   66 TRSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVRSPVNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKLGQVKRSLDSTF 602
            TR V VP +R  PL+  W  I   L   L LQ       R+  +       D   LQKG +F+ AF  GF+  D  +L+R+D LY+++  I+D+   + G+H +RAIGRI G +G ++  +ENATR RIV++D   H++G Y+ + VA   V +L  G+   KV G L +V   +   F
Sbjct:   82 TRKVPVPPHRFSPLKASWSRIYPPLVEHLKLQVRMNIKSRAVELRTSKFTTDTGALQKGEDFVKAFTLGFDVDDAIALLRLDDLYIRSFEIRDVKASLHGEHLSRAIGRIAGKDGKLKHSIENATRTRIVLADQKIHLLGGYRNILVAQEAVVSLILGSPPGKVYGNLRKVASRMKERF 260

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BLAST of RNA-binding protein pno1 vs. UniProt/SwissProt
Match: sp|Q753C6|PNO1_ASHGO (Pre-rRNA-processing protein PNO1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=PNO1 PE=3 SV=3)

HSP 1 Score: 118.627 bits (296), Expect = 2.327e-31
Identity = 69/188 (36.70%), Postives = 96/188 (51.06%), Query Frame = 3

Query:   66 TRSVNVPVNRIKPLREKWVDI---------AKVXXXXXXXXXXXXNHKRSFTVSVRSPVNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKLGQVKRSLDSTF 602
            +R V VP +R+ PLR  W  I          +V             H R  T        D   LQKG +F+ AF  GF+  D  SL+R+D LY++T  IKD+ + + GDH +RAIGRI G +G  +  +ENATR RIV++DS  HI+G +  + +A   V +L  G+   KV G L  V   L   +
Sbjct:   89 SRKVPVPPHRMTPLRNSWTKIYPPLVDHLKLQVRMNLKTKSVELRTHPRHTT--------DPGALQKGADFIKAFTLGFDLDDSISLLRLDDLYIETFEIKDV-KTLHGDHLSRAIGRIAGKDGKTKFAIENATRTRIVLADSKIHILGGFTHIRMAREAVVSLILGSPPGKVYGNLRTVASRLKERY 267

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BLAST of RNA-binding protein pno1 vs. UniProt/SwissProt
Match: sp|O18216|PNO1_CAEEL (RNA-binding protein pno-1 OS=Caenorhabditis elegans OX=6239 GN=pno-1 PE=3 SV=1)

HSP 1 Score: 118.627 bits (296), Expect = 2.842e-31
Identity = 69/170 (40.59%), Postives = 104/170 (61.18%), Query Frame = 3

Query:   66 TRSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVRSPVN--DEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKL 569
            +R V VP +R  PL++ WV+I   +   LGLQ      KR   V +R+PV+  D   LQK  +F+ AF  GFE  D  +LIR+D L+++T  + D+   +KGDH +RAIGRI G +G  +  +EN T+ RIV++++  HI+G+YQ + +A   V +L  G+  +KV G L
Sbjct:   99 SRVVPVPKHRYTPLKDNWVNIFTPIVKNLGLQVRFNLKKRQ--VEIRNPVDREDTTDLQKATDFVRAFILGFEVNDAIALIRLDHLFLETFEVADVKHSLKGDHVSRAIGRIAGKDGRTKLVIENTTKTRIVVANTKIHILGAYQNLKLARNAVCSLILGSNPSKVYGSL 266

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BLAST of RNA-binding protein pno1 vs. UniProt/SwissProt
Match: sp|Q6BJ75|PNO1_DEBHA (Pre-rRNA-processing protein PNO1 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=PNO1 PE=3 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 3.807e-31
Identity = 66/176 (37.50%), Postives = 99/176 (56.25%), Query Frame = 3

Query:   42 SNQQTNLITRSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVRSPVNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKL 569
            S  +  L +R V VP +R+ PL+  W  I   L   L LQ       ++  +       D+  LQKG +F+ AF  GF+  D  +L+R+D LY++T  +KD+ + + GDH +RAIGRI G +G  +  +ENATR RIV++DS  HI+G +  + +A   V +L  G+   KV G L
Sbjct:   70 SGMKVKLESRKVPVPPHRMTPLKNTWTKIYPPLVDHLKLQVRMNLKTKTIEMKTNKNTVDQGALQKGADFVKAFTLGFDVDDAIALLRLDDLYIETFEVKDV-KTLNGDHLSRAIGRIAGKDGKTKFAIENATRTRIVLADSKIHILGGFTHIRMAREAVVSLILGSPPGKVYGNL 244

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BLAST of RNA-binding protein pno1 vs. TrEMBL
Match: U6J8M4 (RNA-binding protein pno1 OS=Echinococcus granulosus OX=6210 GN=EGR_05299 PE=4 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 6.553e-42
Identity = 79/180 (43.89%), Postives = 119/180 (66.11%), Query Frame = 3

Query:   69 RSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVR-SP-VNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKLGQVKRSLDSTF 602
            R+V VP NR  PL+E+W  IA++L+ QL L       K+++ V +R SP   D   LQ+G++F+ AF KGF   D  +++R+DG+YV++ +I D+ + ++GDH  RA+GRICGANG +R  +ENATR RI+++D T HI+GS + + VA + +  L  G+  NKV G++      LDSTF
Sbjct:    5 RTVLVPQNRRGPLKEQWKSIAEILTKQLKLMVCVRTPKKTWRVMLRNSPETADAQHLQRGVDFVSAFVKGFSIQDALAIVRIDGIYVESFHIADVRQRLRGDHMARAVGRICGANGRIRLAIENATRTRIIVADQTIHILGSNERIAVARKAICNLIIGSPPNKVFGRIQNQTARLDSTF 184

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BLAST of RNA-binding protein pno1 vs. TrEMBL
Match: A0A0R3W8A3 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS6574 PE=4 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 1.510e-41
Identity = 80/180 (44.44%), Postives = 118/180 (65.56%), Query Frame = 3

Query:   69 RSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVR-SPVNDEVK-LQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKLGQVKRSLDSTF 602
            R+V VP NR  PL+E W    K+L+ QL L      H R++ V +R SP  D+ + LQ+G++F+ AF KGF   D  +++R+DG+YV++ +I D+ + ++GDH  RAIGRICGANG +R  +ENATR RI+++D T HI+GS + + VA + +  L  G+  NKV G++      LDSTF
Sbjct:    5 RTVLVPANRRSPLKEHW----KILTKQLKLMVCVKTHSRNWRVLLRNSPETDDSQHLQRGVDFVSAFVKGFSIQDALAIVRIDGIYVESFHIADVRQRLRGDHMARAIGRICGANGRIRLSIENATRTRIIVADQTIHILGSNERIAVARKAICNLIIGSPPNKVFGRIQNQTARLDSTF 180

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BLAST of RNA-binding protein pno1 vs. TrEMBL
Match: A0A087W247 (rRNA processing protein Rrp20 OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000257400 PE=4 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 4.131e-41
Identity = 79/180 (43.89%), Postives = 118/180 (65.56%), Query Frame = 3

Query:   69 RSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVR-SP-VNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKLGQVKRSLDSTF 602
            R+V VP NR  PL+E W  IA++L+ QL L       K+++ V +R SP   D   LQ+G++F+ AF KGF   D  +++R+DG+YV++ +I D+ + ++GDH  RA+GRICGANG +R  +ENATR RI+++D T HI+GS + + VA + +  L  G+  NKV G++      LDSTF
Sbjct:   29 RTVLVPQNRRGPLKEHWKSIAEILTKQLKLMVCVKTPKKTWRVMLRNSPETADAQHLQRGVDFVSAFVKGFSIQDALAIVRIDGIYVESFHIADVRQRLRGDHMARAVGRICGANGRIRLAIENATRTRIIVADQTIHILGSNERIAVARKAICNLIIGSPPNKVFGRIQNQTARLDSTF 208

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BLAST of RNA-binding protein pno1 vs. TrEMBL
Match: A0A0R3ULC2 (KH domain-containing protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS8467 PE=4 SV=1)

HSP 1 Score: 147.132 bits (370), Expect = 7.513e-41
Identity = 78/180 (43.33%), Postives = 116/180 (64.44%), Query Frame = 3

Query:   69 RSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVR-SP-VNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKLGQVKRSLDSTF 602
            R++ VP NR  PL++ W  IA++L+ QL L       KR + V +R SP   D   L KG++F+ AF KGF   D  +++R+DG+YV++ +I D+ + ++GDH  RA+GRICGANG +R  +ENATR RIV++D T HI+GS + + VA + +  L  G+  NK+ G++      LDSTF
Sbjct:    5 RTILVPQNRRSPLKDSWKTIAEILTKQLKLMVCVKTSKRHWKVMLRTSPETTDAQHLHKGVDFVSAFIKGFTVQDALAIVRIDGIYVESFHISDVRQRLRGDHMARAVGRICGANGRVRLSIENATRTRIVVADQTIHILGSNERIAVARKAICNLIIGSPPNKIFGRVQNQAARLDSTF 184

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BLAST of RNA-binding protein pno1 vs. TrEMBL
Match: A0A3P7L9Z3 (KH_8 domain-containing protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS6131 PE=4 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 1.993e-40
Identity = 78/180 (43.33%), Postives = 117/180 (65.00%), Query Frame = 3

Query:   69 RSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVR-SP-VNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKLGQVKRSLDSTF 602
            R+V VP NR  PL+E W  I ++L+ QL L       K+++ V +R SP   D   LQKG++F+ AF KGF   D  +++R+DG+YV++ +I D+ + ++GDH  RA+GRICGANG +R  +ENATR RIV++D T HI+GS + + VA + +  L  G+  +K+ G++      LDSTF
Sbjct:    5 RTVLVPQNRRAPLKESWKSIVEILTKQLKLMVCMKTAKKNWKVMLRNSPETTDPQFLQKGVDFVSAFVKGFALQDALAIVRIDGIYVESFHISDVRQRLRGDHVARAVGRICGANGRVRLSIENATRTRIVVADQTIHILGSNERIAVARKAICNLIIGSPPSKIFGRIQTQAARLDSTF 184

[Close]

BLAST of RNA-binding protein pno1 vs. Ensembl Cavefish
Match: pno1 (partner of NOB1 homolog [Source:NCBI gene;Acc:103037712])

HSP 1 Score: 101.293 bits (251), Expect = 5.498e-26
Identity = 57/167 (34.13%), Postives = 92/167 (55.09%), Query Frame = 3

Query:   69 RSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVRSPVNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKL 569
            R + VP +R  PL++ W+ I   +   L LQ       R+  +       D   L K  +F+ AF  GF+  D  +LIR+D L+++T  + D+ + +KGDH  RA+GRI G  G  +  +EN T+ RIV++D+  HI+GS+Q + +A   +  L  G+  +KV G +
Sbjct:   62 RKIPVPSHRYTPLKDNWLKIFTPIVENLQLQVRFNLKTRNVEIKTCKETQDIGALTKAADFVKAFVLGFQVEDALALIRLDELFLETFDVTDV-KPLKGDHLCRAVGRIAGKGGKTKFTIENVTKTRIVLADTKVHILGSFQNIKMARTAICNLILGSPPSKVYGNI 227

[Close]

BLAST of RNA-binding protein pno1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004119.1 (pep scaffold:Pmarinus_7.0:GL483005:2857:6290:1 gene:ENSPMAG00000003755.1 transcript:ENSPMAT00000004119.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 111.694 bits (278), Expect = 1.562e-30
Identity = 63/170 (37.06%), Postives = 95/170 (55.88%), Query Frame = 3

Query:   69 RSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVRSPVNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKLGQV 578
            R V VP NR  PL+E W+ I   +   L LQ       R+  +       D   LQK  +F+ AF  GF+  D  +L+R+D L++++  I D+ + +KGDH +RA+GRI G  G  +  +EN TR RIV++D+  HI+GS+Q + +A   +  L  G+  +KV G L  V
Sbjct:   64 RKVPVPANRFTPLKENWMKIFTPVVEHLQLQIRFNLKTRNVELKTCKETQDIGALQKAADFVRAFVLGFQVEDALALMRLDDLFLESFDITDV-KPLKGDHLSRAVGRIAGKGGKTKFTIENVTRSRIVLADTKVHILGSFQNIKIARTAICNLISGSPPSKVYGNLRAV 232

[Close]

BLAST of RNA-binding protein pno1 vs. Ensembl Yeast
Match: PNO1 (Essential nucleolar protein required for pre-18S rRNA processing; interacts with Dim1p, an 18S rRNA dimethyltransferase, and also with Nob1p, which is involved in proteasome biogenesis; contains a KH domain [Source:SGD;Acc:S000005671])

HSP 1 Score: 115.161 bits (287), Expect = 8.368e-32
Identity = 71/191 (37.17%), Postives = 102/191 (53.40%), Query Frame = 3

Query:   42 SNQQTNLI---TRSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVRSP-VNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKLGQVKRSLDSTF 602
            S  Q N I   +R + VP +R+ PLR  W  I   L   L LQ       +S  +        D   LQKG +F+ AF  GF+  D  +L+R+D LY++T  +KD+ + + GDH +RAIGRI G +G  +  +ENATR RIV++DS  HI+G +  + +A   V +L  G+   KV G L  V   L   +
Sbjct:   85 SKTQGNKIKFESRKIMVPPHRMTPLRNSWTKIYPPLVEHLKLQVRMNLKTKSVELRTNPKFTTDPGALQKGADFIKAFTLGFDLDDSIALLRLDDLYIETFEVKDV-KTLTGDHLSRAIGRIAGKDGKTKFAIENATRTRIVLADSKIHILGGFTHIRMARESVVSLILGSPPGKVYGNLRTVASRLKERY 274

[Close]

BLAST of RNA-binding protein pno1 vs. Ensembl Nematostella
Match: PNO1 (RNA-binding protein pno1 [Source:UniProtKB/Swiss-Prot;Acc:A7RP64])

HSP 1 Score: 107.842 bits (268), Expect = 6.917e-29
Identity = 60/171 (35.09%), Postives = 96/171 (56.14%), Query Frame = 3

Query:   66 TRSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVRSPVNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKLGQV 578
            TR + VP +R  PL+E W+ I   +   L LQ       R   +      +D   +QK  +F+ AF  GFE  D  +LIR+D L++++  I D+ + +KGDH +RAIGR+ G  G  +  +EN T+ RIV++++  HI+GS+Q + +A   +  L  G+  +KV G +  V
Sbjct:   61 TRKIPVPSHRYTPLKENWMKIFTPVVEHLKLQIRFNLGSRHVEIRASKETSDIGAVQKAADFVQAFILGFEVEDALALIRLDDLFLESFEIADV-KPLKGDHLSRAIGRVAGKGGKTKFTIENVTKTRIVLAETKIHILGSFQNIKIARTAICNLILGSPPSKVYGNMRAV 230

[Close]

BLAST of RNA-binding protein pno1 vs. Ensembl Medaka
Match: pno1 (partner of NOB1 homolog [Source:NCBI gene;Acc:100049395])

HSP 1 Score: 104.76 bits (260), Expect = 3.129e-27
Identity = 60/170 (35.29%), Postives = 94/170 (55.29%), Query Frame = 3

Query:   69 RSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVRSPVNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKLGQV 578
            R V VP +R  PL+E W+ I   +   L LQ       R+  +       D   L K  +F+ AF  GF+  D  +LIR+D L++++  + D+ + +KGDH +RAIGRI G  G  +  +EN T+ RIV++D+  HI+GS+Q + +A   +  L  G+  +KV G +  V
Sbjct:   67 RKVAVPAHRYTPLKENWLKIFTPIVENLQLQVRFNLKTRNVEIKTCKETQDIAALTKAADFVKAFVLGFQVDDAMALIRLDELFLESFDVTDV-KPLKGDHLSRAIGRIAGKGGKTKFTIENVTKTRIVLADTKIHILGSFQNIKMARTAICNLILGSPPSKVYGNIRAV 235

[Close]

BLAST of RNA-binding protein pno1 vs. Planmine SMEST
Match: SMESG000015341.1 (SMESG000015341.1)

HSP 1 Score: 357.836 bits (917), Expect = 1.933e-127
Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 3

Query:   39 MSNQQTNLITRSVNVPVNRIKPLREKWVDIAKVXXXXXXXXXXXXNHKRSFTVSVRSPVNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKLGQVKRSLDSTF 602
            MSNQQTNLITRSVNVPVNRIKPLREKWVDIAKVLSLQLGLQSLAANHKRSFTVSVRSPVNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKLGQVKRSLDSTF
Sbjct:    1 MSNQQTNLITRSVNVPVNRIKPLREKWVDIAKVLSLQLGLQSLAANHKRSFTVSVRSPVNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYIKDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGSYQAVDVACRKVGALARGALINKVAGKLGQVKRSLDSTF 188

The following BLAST results are available for this feature:

BLAST of RNA-binding protein pno1 vs. Ensembl Human
BLAST of RNA-binding protein pno1 vs. Ensembl Celegans
BLAST of RNA-binding protein pno1 vs. Ensembl Fly
BLAST of RNA-binding protein pno1 vs. Ensembl Zebrafish
BLAST of RNA-binding protein pno1 vs. Ensembl Xenopus
BLAST of RNA-binding protein pno1 vs. Ensembl Mouse
BLAST of RNA-binding protein pno1 vs. UniProt/SwissProt
BLAST of RNA-binding protein pno1 vs. TrEMBL
BLAST of RNA-binding protein pno1 vs. Ensembl Cavefish
BLAST of RNA-binding protein pno1 vs. Ensembl Sea Lamprey
BLAST of RNA-binding protein pno1 vs. Ensembl Yeast
BLAST of RNA-binding protein pno1 vs. Ensembl Nematostella
BLAST of RNA-binding protein pno1 vs. Ensembl Medaka
BLAST of RNA-binding protein pno1 vs. Planmine SMEST

BLAST of RNA-binding protein pno1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1


Match Name	E-value	Identity	Description
PNO1	4.638e-28	37.85	partner of NOB1 homolog [Source:HGNC Symbol;Acc:HG...	[more]

BLAST of RNA-binding protein pno1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2


Match Name	E-value	Identity	Description
pno-1	2.234e-32	40.59	RNA-binding protein pno-1 [Source:UniProtKB/Swiss...	[more]
pno-1	2.234e-32	40.59	RNA-binding protein pno-1 [Source:UniProtKB/Swiss...	[more]

BLAST of RNA-binding protein pno1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1


Match Name	E-value	Identity	Description
l(1)G0004	1.276e-30	37.93	gene:FBgn0027334 transcript:FBtr0077298	[more]

BLAST of RNA-binding protein pno1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
pno1	3.608e-27	35.93	partner of NOB1 homolog [Source:NCBI gene;Acc:3935...	[more]

BLAST of RNA-binding protein pno1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ehf	1.992e-27	35.88	ETS homologous factor [Source:Xenbase;Acc:XB-GENE-...	[more]

BLAST of RNA-binding protein pno1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1


Match Name	E-value	Identity	Description
Pno1	9.750e-28	37.85	partner of NOB1 homolog [Source:MGI Symbol;Acc:MGI...	[more]

BLAST of RNA-binding protein pno1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q5B0Z5\|PNO1_EMENI	7.435e-32	37.43	Pre-rRNA-processing protein pno1 OS=Emericella nid...	[more]
sp\|Q2UMG1\|PNO1_ASPOR	1.443e-31	36.87	Pre-rRNA-processing protein pno1 OS=Aspergillus or...	[more]
sp\|Q753C6\|PNO1_ASHGO	2.327e-31	36.70	Pre-rRNA-processing protein PNO1 OS=Ashbya gossypi...	[more]
sp\|O18216\|PNO1_CAEEL	2.842e-31	40.59	RNA-binding protein pno-1 OS=Caenorhabditis elegan...	[more]
sp\|Q6BJ75\|PNO1_DEBHA	3.807e-31	37.50	Pre-rRNA-processing protein PNO1 OS=Debaryomyces h...	[more]

BLAST of RNA-binding protein pno1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
U6J8M4	6.553e-42	43.89	RNA-binding protein pno1 OS=Echinococcus granulosu...	[more]
A0A0R3W8A3	1.510e-41	44.44	Uncharacterized protein OS=Taenia asiatica OX=6051...	[more]
A0A087W247	4.131e-41	43.89	rRNA processing protein Rrp20 OS=Echinococcus mult...	[more]
A0A0R3ULC2	7.513e-41	43.33	KH domain-containing protein OS=Mesocestoides cort...	[more]
A0A3P7L9Z3	1.993e-40	43.33	KH_8 domain-containing protein OS=Dibothriocephalu...	[more]

BLAST of RNA-binding protein pno1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
pno1	5.498e-26	34.13	partner of NOB1 homolog [Source:NCBI gene;Acc:1030...	[more]

BLAST of RNA-binding protein pno1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSPMAT00000004119.1	1.562e-30	37.06	pep scaffold:Pmarinus_7.0:GL483005:2857:6290:1 gen...	[more]

BLAST of RNA-binding protein pno1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
PNO1	8.368e-32	37.17	Essential nucleolar protein required for pre-18S r...	[more]

BLAST of RNA-binding protein pno1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
PNO1	6.917e-29	35.09	RNA-binding protein pno1 [Source:UniProtKB/Swiss-...	[more]

BLAST of RNA-binding protein pno1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
pno1	3.129e-27	35.29	partner of NOB1 homolog [Source:NCBI gene;Acc:1000...	[more]

BLAST of RNA-binding protein pno1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000015341.1	1.933e-127	100.00	SMESG000015341.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30027596 ID=SMED30027596|Name=RNA-binding protein pno1|organism=Schmidtea mediterranea sexual|type=transcript|length=660bp
GTTTAAAAAATGTTTAAATATTACTTTTAAATTAAATCATGTCAAATCAA
CAAACTAATCTTATAACTAGAAGCGTAAATGTACCTGTGAATCGTATTAA
GCCCCTAAGGGAAAAGTGGGTAGATATTGCCAAAGTTTTAAGTTTACAAT
TGGGTTTACAATCTCTTGCTGCGAATCATAAAAGATCATTTACTGTTTCT
GTACGATCACCAGTAAATGACGAAGTCAAACTTCAAAAAGGAATTGAATT
TTTAGATGCTTTTTGTAAAGGATTTGAATTTGTTGATTGCACATCCTTAA
TCAGAATGGATGGATTATACGTAAAAACATTGTATATTAAAGACATAAAC
CGAGTTATCAAGGGCGATCATTTCACTAGAGCAATTGGTAGAATATGTGG
TGCCAATGGGATTATGCGTACAAAATTAGAAAACGCTACTCGATGCAGGA
TTGTTATTTCTGATTCTACTGCACATATTATGGGTTCCTATCAGGCAGTT
GATGTTGCATGCAGGAAGGTTGGTGCACTCGCCAGAGGTGCACTTATAAA
CAAAGTAGCCGGAAAATTAGGACAAGTTAAAAGAAGTCTTGACTCTACTT
TTTAATTATTTATTATAATACATAATTGATGTTGAATAAAATTTAAATTG
AGGAAAATAA

protein sequence of SMED30027596-orf-1

>SMED30027596-orf-1 ID=SMED30027596-orf-1|Name=SMED30027596-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=189bp
MSNQQTNLITRSVNVPVNRIKPLREKWVDIAKVLSLQLGLQSLAANHKRS
FTVSVRSPVNDEVKLQKGIEFLDAFCKGFEFVDCTSLIRMDGLYVKTLYI
KDINRVIKGDHFTRAIGRICGANGIMRTKLENATRCRIVISDSTAHIMGS
YQAVDVACRKVGALARGALINKVAGKLGQVKRSLDSTF*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000026	gut

Vocabulary: **INTERPRO**
Term	Definition
IPR036612	KH_dom_type_1_sf
IPR004088	KH_dom_type_1

Vocabulary: **molecular function**
Term	Definition
GO:0003723	RNA binding
GO:0003676	nucleic acid binding
GO:0051082	unfolded protein binding

Vocabulary: **biological process**
Term	Definition
GO:0000447	endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0000472	endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
GO:0006461	protein complex assembly
GO:0042254	ribosome biogenesis

Vocabulary: **cellular component**
Term	Definition
GO:0005634	nucleus
GO:0005730	nucleolus
GO:0005737	cytoplasm
GO:0005829	cytosol

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036612	K Homology domain, type 1 superfamily	GENE3D	G3DSA:3.30.1370.10		coord: 1..62 e-value: 2.0E-9 score: 39.3
IPR036612	K Homology domain, type 1 superfamily	SUPERFAMILY	SSF54791	Eukaryotic type KH-domain (KH-domain type I)	coord: 2..52