SMED30027560

Overview
NameSMED30027560
Smed IDSMED30027560
Length (bp)1134
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30027560 (SMED30027560) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30027560 (SMED30027560) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30027560 (SMED30027560) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30027560 (SMED30027560) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30027560

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30027560 SmedASXL_070195SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
X1 cellSMED30027560 SmedASXL_070195SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30027560 SmedASXL_070195SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30027560SMESG000080985.1 dd_Smed_v4_22202_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30027560SMESG000080985.1 dd_Smed_v4_22202_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30027560 vs. TrEMBL
Match: A0A2G8KB92 (Integrase catalytic domain-containing protein OS=Stichopus japonicus OX=307972 GN=BSL78_17882 PE=4 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 2.148e-46
Identity = 96/249 (38.55%), Postives = 131/249 (52.61%), Query Frame = -1 
Query:  385 LYSTREESTVHIEMAYELDMDAFLQAFMRFANRRGMPKIFLSNNGTNFVGVLSHVGNFA--IDNYEVSTKLKENECCWIFNPPSAPRFGGVFETLIKSEKIAMKAIMISADVNEEELTNVFVRAEMLLHSRPLLTQCIDSREDVIITPNHLLMIAEWRSEMNINTAFLGKNELRLRWRRIQELAQAFWKRWLTK---LTRQN*NF---QEEIKIGSVVWVIDKKNNYGKWHIGKVIENYPGKIGKVRVV 1107
             ++      VH+EMAY LD D+FL A  R  NRRG+P   +S+NGTNF G    +      +D  +++         W FNPP +P FGGV ET+IK  K A+K ++  AD+ +EELT VF  AE LL+SRPL  Q  DS+                  +  ++T   G    R RWRR+QEL + FW RWL +   L  Q   +   Q ++K+G VV V       G W +G+++E  PG  G VRVV
Sbjct:   44 FFTCLSSRAVHLEMAYGLDTDSFLNALNRMINRRGVPVEMISDNGTNFKGAEKKLRELVDRMDKDKMTRSTANQMIKWHFNPPLSPHFGGVHETMIKGAKKAIKTVVYKADITDEELTTVFTSAEALLNSRPLTYQSADSQAG---------------GQFAVDTVDDGSFSPRKRWRRVQELVRHFWHRWLKEWLPLLNQRRKWRLPQRDLKVGDVVIVYSSDTPRGSWPLGRIVEVLPGVDGHVRVV 277
[Close]
BLAST of SMED30027560 vs. TrEMBL
Match: A0A5S6Q5N7 (Uncharacterized protein OS=Trichuris muris OX=70415 PE=4 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 4.597e-46
Identity = 94/244 (38.52%), Postives = 138/244 (56.56%), Query Frame = -1 
Query:  379 VHIEMAYELDMDAFLQAFMRFANRRGMPKIFLSNNGTNFVGVLSHVGNF--AIDNYEVSTKLKENECCWIFNPPSAPRFGGVFETLIKSEKIAMKAIMISADVNEEELTNVFVRAEMLLHSRPLLTQCIDSREDVIITPNHLLMIAEWRSEMNINTAFLGKNEL--RLRWRRIQELAQAFWKRWLTKLT------RQN*NFQEEIKIGSVVWVIDKKNNYGKWHIGKVIENYPGKIGKVRVVII 1080
            VH+EM+Y LD D+FL AF RF +RRG P +  S+NGTN V     V     + +  +++  L ++   W+F+PP+AP FGG +E L++S KIA++ I+    V +E L   FV  E LL+SRPL    +D R+   +TPNH L+    R   +I    + + E+  R RWR  Q + ++FWKRWL          R+      ++K+G VV V+D +   G W +G+V E  PGK G VRV  +
Sbjct:  137 VHLEMSYSLDTDSFLMAFRRFISRRGSPAVVYSDNGTNLVAAEKEVRTSLESWNQAKIAEALTQHRIQWVFSPPNAPHFGGAWERLVRSAKIALRKILNGRAVTDETLVTAFVEIEGLLNSRPLTHISVDPRDPEPLTPNHFLL---GRPHPHIPPDVINEQEITSRRRWRIAQAIVESFWKRWLRGYVPCLIERRKWVRPTRDLKVGDVVIVVDPQCPRGHWPLGRVTECRPGKDGIVRVAKV 377
[Close]
BLAST of SMED30027560 vs. TrEMBL
Match: A0A2G8L9D8 (Integrase catalytic domain-containing protein OS=Stichopus japonicus OX=307972 GN=BSL78_06265 PE=4 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 7.561e-45
Identity = 101/261 (38.70%), Postives = 148/261 (56.70%), Query Frame = -1 
Query:  358 LYSTREESTVHIEMAYELDMDAFLQAFMRFANRRGMPKIFLSNNGTNFVGVLSHVGNF--AIDNYEVSTKLKENECCWIFNPPSAPRFGGVFETLIKSEKIAMKAIMISADVNEEELTNVFVRAEMLLHSRPLLTQCIDSREDVIITPNHLL---MIAEWRSEMNINTAFLGKNELRLRWRRIQELAQAFWKR----WLTKLTRQN*NFQE--EIKIGSVVWVIDKKNNYGKWHIGKVIENYPGKIGKVRVVIILCEGKEV 1107
            L++      VH+E+AY LD D FL AF RF +RRG P+   S+NGTNFVG    +      ID  +V  ++   +  W F PP +P FGG  E LIK+ K A++A++  ADV +EEL + FV AE +++SRPL       ++   +TPNH L   M   + SE  +++    K   R RWRR+QEL + FW+R    WL  L R+    +E  ++K+  +V V++K    G+W +G++ E +PG  G +RVV I     EV
Sbjct:  133 LFTCLSTRAVHLEIAYGLDTDTFLNAFYRFVSRRGEPEEVFSDNGTNFVGASRELKELFRLIDKSKVQ-EVTAGQMRWHFLPPLSPHFGGAHEALIKTSKRAIQAVLGDADVTDEELHSAFVGAEGIINSRPLTYVSASPKDMTPLTPNHFLHGSMGGTFASEEIVDST---KYSPRRRWRRVQELMRHFWQRWLREWLPSLNRRKKWHKEKVDLKVDDLVLVMEKDVVRGQWPLGRIKEVFPGPDGHIRVVKIKTSRGEV 389
[Close]
BLAST of SMED30027560 vs. TrEMBL
Match: A0A5S6Q1B8 (Uncharacterized protein OS=Trichuris muris OX=70415 PE=4 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 1.878e-43
Identity = 88/230 (38.26%), Postives = 132/230 (57.39%), Query Frame = -1 
Query:  421 VHIEMAYELDMDAFLQAFMRFANRRGMPKIFLSNNGTNFVGVLSHVGNF--AIDNYEVSTKLKENECCWIFNPPSAPRFGGVFETLIKSEKIAMKAIMISADVNEEELTNVFVRAEMLLHSRPLLTQCIDSREDVIITPNHLLMIAEWRSEMNINTAFLGKNEL--RLRWRRIQELAQAFWKRWLTKLT------RQN*NFQEEIKIGSVVWVIDKKNNYGKWHIGKVIE 1080
            VH+EM+Y LD D+FL AF RF +RRG P +  S+NGTN V     V     + +  +++  L ++   W+F+PP+AP FGGV+E L++S KIA++ I+    V +E L   FV  E LL+SRPL    +D R+   +TPNH L+    R   +I    + + E+  R RWR  Q + ++FWKRWL +        R+      ++K+G VV V+D +   G W +G+V E
Sbjct:  137 VHLEMSYSLDTDSFLMAFRRFISRRGSPAVVYSDNGTNLVAAEKEVRTSLESWNQAKIAEALTQHRIQWVFSPPNAPHFGGVWERLVRSAKIALRKILNGRAVTDETLVTAFVEIEGLLNSRPLTHISVDPRDPEPLTPNHFLL---GRPHPHIPPDVINEQEITSRRRWRIAQAIVESFWKRWLREYVPCLIERRKWVRPTRDLKVGDVVIVVDPQCPRGHWPLGRVTE 363
[Close]
BLAST of SMED30027560 vs. TrEMBL
Match: A0A085NQS3 (Integrase catalytic domain-containing protein OS=Trichuris suis OX=68888 GN=M514_10175 PE=4 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 2.293e-40
Identity = 95/258 (36.82%), Postives = 139/258 (53.88%), Query Frame = -1 
Query:  379 LYSTREESTVHIEMAYELDMDAFLQAFMRFANRRGMPKIFLSNNGTNFVGVLSHVGNF--AIDNYEVSTKLKENECCWIFNPPSAPRFGGVFETLIKSEKIAMKAIMISADVNEEELTNVFVRAEMLLHSRPLLTQCIDSREDVIITPNHLLM-------IAEWRSEMNINTAFLGKNELRLRWRRIQELAQAFWKRWLTKLT------RQN*NFQEEIKIGSVVWVIDKKNNYGKWHIGKVIENYPGKIGKVRVVII 1107
            L++      VHIE+A+ L+ D+FL AF RF +RRG P +  S+NGTN V     +     + D  +V   +  +   W F+PPSAP FGGV+E +I+S K A+  I+     ++E L  VFV  E LL+SRPL    IDS +   +TPNH L+         +  +E++I++        R +WR  Q + ++FW+RWL +        R+    +  +K G +V V+D +   G W  G VIE YPGK G VRV  I
Sbjct: 1479 LFTCLSTRAVHIEIAFTLNTDSFLMAFRRFMDRRGTPSVVYSDNGTNLVAAEKELRKCLESWDQLKVHENVVRHGIQWHFSPPSAPHFGGVWERMIRSAKAALHKILNGRATSDEVLLTVFVEVESLLNSRPLTHISIDSSDPEPLTPNHFLLGRPCPHVPPDVVTEIDISS--------RRKWRAAQAIVESFWRRWLREYVPSLIERRKWLRARRNLKKGDIVVVVDPRTPRGHWPFGTVIECYPGKDGIVRVAKI 1728
[Close]
BLAST of SMED30027560 vs. Planmine SMEST
Match: SMESG000041215.1 (SMESG000041215.1)

HSP 1 Score: 227.254 bits (578), Expect = 7.083e-66
Identity = 112/260 (43.08%), Postives = 169/260 (65.00%), Query Frame = -1 
Query:  346 LYSTREESTVHIEMAYELDMDAFLQAFMRFANRRGMPKIFLSNNGTNFVGVLSHVGNFAIDNYEVSTKLKENECCWIFNPPSAPRFGGVFETLIKSEKIAMKAIMISADVNEEELTNVFVRAEMLLHSRPLLTQCIDSREDVIITPNHLLMIAEWRSEMNINTAFLGKNELRLRWRRIQELAQAFWKRWLTKLT------RQN*NFQEEIKIGSVVWVIDKKNNYGKWHIGKVIENYPGKIGKVRVVIILCEGKEVKIHC 1107
            L++  +   VH+E+A+ LD DAF++AF+RF  RR  P + LS+NGTNFVG  + V    +D+Y  +  +  N+  WIFNPP AP FGGVFE LI+S K A++ I+ SAD+++EEL +VF++ E LL++RPL  QC DS+ED+++TPNH L++ + ++E  I     G +  R RWRRI E+   FW+RW  ++       R+  N +  I +G +VW+++K    GKW + ++ E  PG+ G+VRVV I  +GK +K  C
Sbjct: 1373 LFTCLQTRAVHLEVAHALDTDAFIRAFIRFMGRREKPLMILSDNGTNFVGATNKVQEIKVDSYRFNKFMSGNDIIWIFNPPLAPHFGGVFEALIESAKKALQVIVGSADLSDEELNSVFIKVESLLNARPLTIQCSDSKEDLVLTPNHFLLLKQPQNEDEILNPKTGAS-YRRRWRRILEVTDHFWERWKREIVPLWSTRRKWWNEKNNIAVGDIVWLLEKSTYDGKWPLARICEVVPGQDGRVRVVKIRVDGK-IKYPC 1630
[Close]
BLAST of SMED30027560 vs. Planmine SMEST
Match: SMESG000005924.1 (SMESG000005924.1)

HSP 1 Score: 221.476 bits (563), Expect = 2.464e-64
Identity = 117/270 (43.33%), Postives = 173/270 (64.07%), Query Frame = -1 
Query:  343 LKEMSRV*HLYSTREESTVHIEMAYELDMDAFLQAFMRFANRRGMPKIFLSNNGTNFVGVLSHVGNFAIDNYEVSTKLKENECCWIFNPPSAPRFGGVFETLIKSEKIAMKAIMISADVNEEELTNVFVRAEMLLHSRPLLTQCIDSREDVIITPNHLLMIAEWRSEMNINTAFLGKNELRLRWRRIQELAQAFWKRW------LTKLTRQN*NFQEEIKIGSVVWVIDKKNNYGKWHIGKVIENYPGKIGKVRVVIILCEGKEVKIHCK 1134
            L E++R   +   R+E  VH+E+AY LD DAF++AF+RF  RRG P + LS+NGTNFVG  + V    +D+Y ++  +  N+  WIFNPP AP FG VFE LIKS K A++ I+ SAD+++EEL +VF++ E LL++RPL TQC DS+ED+++TPNH L++ + ++E  I     G +  R RWRRI E+   FW+ W      L    R+  N +  I +G +VW+++K    GK  + ++ E  PG+ G+VRVV I  +G   K+ C+
Sbjct:  731 LGELNREYWVVCARKE--VHLEVAYALDTDAFIRAFIRFMGRRGKPLMILSDNGTNFVGATNEVQEIKVDSYRLNKFMSGNDIRWIFNPPLAPHFGEVFEALIKSAKKALQVIVGSADLSDEELNSVFIKVESLLNARPLTTQCSDSKEDLVLTPNHFLLLKQPQNEDEILNPKNGAS-YRKRWRRILEVTDHFWEHWKREIVPLWSTRRKWWNEKNNIAVGDIVWLLEKSTYNGKRPLARICEVVPGQDGRVRVVKIRVDG---KVKCR 994
[Close]
BLAST of SMED30027560 vs. Planmine SMEST
Match: SMESG000005924.1 (SMESG000005924.1)

HSP 1 Score: 219.935 bits (559), Expect = 9.517e-64
Identity = 112/252 (44.44%), Postives = 164/252 (65.08%), Query Frame = -1 
Query:  343 VHIEMAYELDMDAFLQAFMRFANRRGMPKIFLSNNGTNFVGVLSHVGNFAIDNYEVSTKLKENECCWIFNPPSAPRFGGVFETLIKSEKIAMKAIMISADVNEEELTNVFVRAEMLLHSRPLLTQCIDSREDVIITPNHLLMIAEWRSEMNINTAFLGKNELRLRWRRIQELAQAFWKRW------LTKLTRQN*NFQEEIKIGSVVWVIDKKNNYGKWHIGKVIENYPGKIGKVRVVIILCEGKEVKIHCK 1080
            VH+E+AY LD DAF++AF+RF  RRG P + LS+NGTNFVG  + V    +D+Y ++  +  N+  WIFNPP AP FG VFE LIKS K A++ I+ SAD+++EEL +VF++ E LL++RPL TQC DS+ED+++TPNH L++ + ++E  I     G +  R RWRRI E+   FW+ W      L    R+  N +  I +G +VW+++K    GK  + ++ E  PG+ G+VRVV I  +G   K+ C+
Sbjct:  755 VHLEVAYALDTDAFIRAFIRFMGRRGKPLMILSDNGTNFVGATNEVQEIKVDSYRLNKFMSGNDIRWIFNPPLAPHFGEVFEALIKSAKKALQVIVGSADLSDEELNSVFIKVESLLNARPLTTQCSDSKEDLVLTPNHFLLLKQPQNEDEILNPKNGAS-YRKRWRRILEVTDHFWEHWKREIVPLWSTRRKWWNEKNNIAVGDIVWLLEKSTYNGKRPLARICEVVPGQDGRVRVVKIRVDG---KVKCR 1002
[Close]
BLAST of SMED30027560 vs. Planmine SMEST
Match: SMESG000063595.1 (SMESG000063595.1)

HSP 1 Score: 212.231 bits (539), Expect = 1.084e-60
Identity = 108/254 (42.52%), Postives = 162/254 (63.78%), Query Frame = -1 
Query:  364 LYSTREESTVHIEMAYELDMDAFLQAFMRFANRRGMPKIFLSNNGTNFVGVLSHVGNFAIDNYEVSTKLKENECCWIFNPPSAPRFGGVFETLIKSEKIAMKAIMISADVNEEELTNVFVRAEMLLHSRPLLTQCIDSREDVIITPNHLLMIAEWRSEMNINTAFLGKNELRLRWRRIQELAQAFWKRWLTKLT------RQN*NFQEEIKIGSVVWVIDKKNNYGKWHIGKVIENYPGKIGKVRVVIILCEGK 1107
            L++  +   VH+E+AY LD DAF+ AF+RF  RR  P + LS+NGTNFV   + V    +D+Y  +  +  N+  WIFNPP AP F GVFE LIKS K A++ I+ SAD+++EEL +VF++ E LL++RPL TQC DS+ED+++TPNH L++ + ++E  I     G +  R+ WRRI E+   FW+RW  ++       R+  N +  I +G +VW+++K    GK  + ++ E  P + G+VRVV I  +GK
Sbjct: 1880 LFTCLKTRAVHLEVAYALDTDAFILAFIRFMGRRRKPLMILSDNGTNFVWATNEVQEIKVDSYRFNKFMSGNDIRWIFNPPLAPHFEGVFEALIKSSKKALQVIVGSADLSDEELNSVFIKIESLLNARPLTTQCSDSKEDLVLTPNHFLLLKQPQNEDEILNPKTGASYRRI-WRRILEVTDHFWERWKREIVPLWSTRRKWWNEKNNIAVGDIVWLLEKSTYDGKGPLARICEVVPSQDGRVRVVKIRVDGK 2132
[Close]
BLAST of SMED30027560 vs. Planmine SMEST
Match: SMESG000000866.1 (SMESG000000866.1)

HSP 1 Score: 187.963 bits (476), Expect = 2.248e-56
Identity = 85/161 (52.80%), Postives = 119/161 (73.91%), Query Frame = -1 
Query:  625 LYSTREESTVHIEMAYELDMDAFLQAFMRFANRRGMPKIFLSNNGTNFVGVLSHVGNFAIDNYEVSTKLKENECCWIFNPPSAPRFGGVFETLIKSEKIAMKAIMISADVNEEELTNVFVRAEMLLHSRPLLTQCIDSREDVIITPNHLLMIAEWRSEMNI 1107
            L+   +   VHIE+AY+LD DAF++AF+RF + + MP++ LS+NGTNFVG  SH+ N  ID+Y V  +L  +EC W+FNPPSA  F GVFE LIKS K  M+A+M+ +DVN+EEL NVF +A+ML++S PL+ Q  D+++D +ITPNH L +A W+SE  +
Sbjct:  163 LFVCLKTRAVHIELAYKLDTDAFIKAFLRFTSLQEMPQLMLSDNGTNFVGAASHLRNIVIDSYMVEKELSRHECRWLFNPPSASHFEGVFEALIKSTKRVMQAVMVDSDVNDEELVNVFTKADMLINSLPLVKQAADTKKDAVITPNH-LAVAGWKSEAAV 322
The following BLAST results are available for this feature:
BLAST of SMED30027560 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30027560 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30027560 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30027560 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30027560 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30027560 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30027560 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30027560 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2G8KB922.148e-4638.55Integrase catalytic domain-containing protein OS=S... [more]
A0A5S6Q5N74.597e-4638.52Uncharacterized protein OS=Trichuris muris OX=7041... [more]
A0A2G8L9D87.561e-4538.70Integrase catalytic domain-containing protein OS=S... [more]
A0A5S6Q1B81.878e-4338.26Uncharacterized protein OS=Trichuris muris OX=7041... [more]
A0A085NQS32.293e-4036.82Integrase catalytic domain-containing protein OS=T... [more]
back to top
BLAST of SMED30027560 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30027560 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30027560 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30027560 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30027560 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30027560 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000041215.17.083e-6643.08SMESG000041215.1[more]
SMESG000005924.12.464e-6443.33SMESG000005924.1[more]
SMESG000005924.19.517e-6444.44SMESG000005924.1[more]
SMESG000063595.11.084e-6042.52SMESG000063595.1[more]
SMESG000000866.12.248e-5652.80SMESG000000866.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027560 ID=SMED30027560|Name=SMED30027560|organism=Schmidtea mediterranea sexual|type=transcript|length=1134bp
GATTTTAGTGGGATTATATTTCATTACTTCAATTATTATAATATAGATCC
AAAATAATATATAATAAAGGCAAGAAATCCAATTCATGAAAACTTATTTT
TATATTTTTCAAATTTCTACCAAATTAACTCAATTGATTTTTTGAGTTTT
TTTAAACCACCAAAAAAATAAAAAATTTAACTTTAATAACACATTTTTAC
AATATTTATTTTACATTAAAAATTAACCACTCTCCCACCCCCCTCTTGAC
ACTCTAATGTTAACCCTTCATCATATATGTTATCTCCCAAGGATTCTATA
CTTTGTTCCTTATTTATCTGATTTTCGTAAATTTTCATCAATTTTACAAT
GGATTTTTACTTCTTTTCCTTCACAAAGAATTATAACTACTCGTACTTTT
CCAATTTTTCCTGGATAATTTTCTATTACTTTTCCGATATGCCATTTTCC
ATAATTGTTTTTCTTATCAATCACCCACACAACAGACCCGATTTTTATCT
CTTCTTGAAAATTTCAGTTTTGTCTTGTCAATTTTGTTAGCCAACGCTTC
CAAAATGCTTGAGCTAATTCTTGAATTCGTCTCCATCTTAACCTTAATTC
GTTCTTTCCCAAAAAAGCAGTGTTTATATTCATTTCACTTCTCCATTCAG
CAATCATCAATAGATGATTTGGTGTAATAATCACATCTTCTCGACTATCA
ATACACTGTGTCAATAGAGGTCTGGAATGCAATAGCATTTCGGCGCGTAC
AAATACGTTCGTCAATTCTTCTTCATTCACATCTGCACTAATCATTATTG
CCTTCATTGCTATTTTTTCGGATTTGATTAATGTTTCAAAAACTCCTCCG
AAACGAGGAGCTGAAGGTGGATTAAAAATCCAACAGCATTCATTTTCTTT
CAATTTAGTTGAGACTTCGTAATTATCGATCGCAAAATTGCCCACATGGG
ATAGCACTCCAACAAAGTTAGTACCATTGTTTGATAGAAATATCTTAGGC
ATTCCTCGTCTATTTGCAAATCTCATAAACGCTTGCAAGAATGCATCCAT
GTCCAATTCATAAGCCATTTCTATATGCACTGTCGACTCTTCACGCGTCG
AATATAAGTGCTAAACTCGCGACATTTCTTTTAA
back to top

protein sequence of SMED30027560-orf-1

>SMED30027560-orf-1 ID=SMED30027560-orf-1|Name=SMED30027560-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=185bp
MAYELDMDAFLQAFMRFANRRGMPKIFLSNNGTNFVGVLSHVGNFAIDNY
EVSTKLKENECCWIFNPPSAPRFGGVFETLIKSEKIAMKAIMISADVNEE
ELTNVFVRAEMLLHSRPLLTQCIDSREDVIITPNHLLMIAEWRSEMNINT
AFLGKNELRLRWRRIQELAQAFWKRWLTKLTRQN*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR012337RNaseH-like_sf
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding