Cathepsin F

Overview
NameCathepsin F
Smed IDSMED30027540
Length (bp)1759
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Cathepsin F (SMED30027540) t-SNE clustered cells

Violin plots show distribution of expression levels for Cathepsin F (SMED30027540) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Cathepsin F (SMED30027540) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Cathepsin F (SMED30027540) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30027540

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 27

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30027540SMESG000005279.1 dd_Smed_v6_456_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pigment cellSMED30027540SMESG000005279.1 dd_Smed_v6_456_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30027540SMESG000005279.1 dd_Smed_v6_456_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
glial cellSMED30027540SMESG000005279.1 dd_Smed_v6_456_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
goblet cellSMED30027540SMESG000005279.1 dd_Smed_v6_456_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30027540SMESG000068156.1 SMESG000064685.1 SMESG000076470.1 SMESG000062429.1 SMESG000053533.1 SMESG000026676.1 SMESG000015751.1 Contig261GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30027540SMESG000005279.1 Contig261GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30027540SMESG000005279.1 dd_Smed_v4_456_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30027540SMESG000005279.1 dd_Smed_v4_456_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30027540SMESG000005279.1 dd_Smed_v4_456_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30027540SMESG000005279.1 dd_Smed_v4_456_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30027540SMESG000005279.1 dd_Smed_v4_456_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30027540SMESG000005279.1 dd_Smed_v4_456_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027540SMESG000005279.1 Contig22451newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027540SMESG000005279.1 Contig22451uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027540SMESG000005279.1 Contig10005newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027540SMESG000005279.1 Contig10005uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027540SMESG000005279.1 Contig14271newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027540SMESG000005279.1 Contig14271uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027540SMESG000005279.1 Contig42673newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027540SMESG000005279.1 Contig42673uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027540SMESG000005279.1 Contig12372newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027540SMESG000005279.1 Contig12372uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027540SMESG000005279.1 Contig44859newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027540SMESG000005279.1 Contig44859uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027540SMESG000005279.1 Contig51139newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027540SMESG000005279.1 Contig51139uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Cathepsin F vs. Ensembl Human
Match: CTSF (cathepsin F [Source:HGNC Symbol;Acc:HGNC:2531])

HSP 1 Score: 295.434 bits (755), Expect = 6.422e-93
Identity = 152/307 (49.51%), Postives = 201/307 (65.47%), Query Frame = 2
Query:  683 FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQAV--------DWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            F +F   +N+ Y+++EE   R  +F +N  +A  +  LD+G+A YGVT+FSDLT+EEFR+ YL+    L K P         +K A++V        DWR+ G VT VK+QG CGSCWAFS TGNVEGQWFL    L+SLSEQ+L+DCD +D  C GGLPS AY AI+ +GGLE+ED Y Y  +  +C     K  VYIN S  +  +E  +A W +  GPIS+ INAF MQFYR GIS P    C+P  +DH VL+VG+ +  S  PFW +KNSWG  WGE GYY ++RGSG CG+N MA+++V++
Sbjct:  187 FKNFVITYNRTYESKEEARWRLSVFVNNMVRAQKIQALDRGTAQYGVTKFSDLTEEEFRTIYLNTL--LRKEPG------NKMKQAKSVGDLAPPEWDWRSKGAVTKVKDQGMCGSCWAFSVTGNVEGQWFLNQGTLLSLSEQELLDCDKMDKACMGGLPSNAYSAIKNLGGLETEDDYSYQGHMQSCNFSAEKAKVYINDSVELSQNEQKLAAWLAKRGPISVAINAFGMQFYRHGISRPLRPLCSPWLIDHAVLLVGYGN-RSDVPFWAIKNSWGTDWGEKGYYYLHRGSGACGVNTMASSAVVD 484          
BLAST of Cathepsin F vs. Ensembl Human
Match: CTSF (cathepsin F [Source:HGNC Symbol;Acc:HGNC:2531])

HSP 1 Score: 226.868 bits (577), Expect = 3.224e-69
Identity = 124/256 (48.44%), Postives = 161/256 (62.89%), Query Frame = 2
Query:  683 FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQAV--------DWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVL 1426
            F +F   +N+ Y+++E    R  +F +N  +A  +  LD+G+A YGVT+FSDLT+EEFR+ YL+    L K P         +K A++V        DWR+ G VT VK+QG CGSCWAFS TGNVEGQWFL    L+SLSEQ+L+DCD +D  C GGLPS AY AI+ +GGLE+ED Y Y  +  +C     K  VYIN S  +  +E  +A W +  GPIS+ INAF MQFYR GIS P    C+P  +DH VL
Sbjct:   36 FKNFVITYNRTYESKEARW-RLSVFVNNMVRAQKIQALDRGTAQYGVTKFSDLTEEEFRTIYLNTL--LRKEPG------NKMKQAKSVGDLAPPEWDWRSKGAVTKVKDQGMCGSCWAFSVTGNVEGQWFLNQGTLLSLSEQELLDCDKMDKACMGGLPSNAYSAIKNLGGLETEDDYSYQGHMQSCNFSAEKAKVYINDSVELSQNEQKLAAWLAKRGPISVAINAFGMQFYRHGISRPLRPLCSPWLIDHAVL 282          
BLAST of Cathepsin F vs. Ensembl Human
Match: CTSL (cathepsin L [Source:HGNC Symbol;Acc:HGNC:2537])

HSP 1 Score: 197.208 bits (500), Expect = 1.919e-57
Identity = 124/306 (40.52%), Postives = 166/306 (54.25%), Query Frame = 2
Query:  692 FKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMN---RLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTI--DNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAF--AMQFYRGGISHPFSIFCNPNHLDHGVLIV--GFNSTTS-GEPFWIVKNSWGESWGENGYYLVYRG-SGVCGLNKMATTSVI 1576
            +K   N+LY   EE  +R  ++  N K   L N   R  K S    +  F D+T EEFR Q ++   +   R  ++ Q P   +  ++VDWR  G VT VKNQG CGSCWAFS TG +EGQ F KT RL+SLSEQ LVDC     + GCNGGL   A++ +Q  GGL+SE+SYPY A + +C  +         G   I   E  +    +  GPIS+ I+A   +  FY+ GI   F   C+   +DHGVL+V  GF ST S    +W+VKNSWGE WG  GY  + +     CG+   A+   +
Sbjct:   32 WKAMHNRLYGMNEEGWRR-AVWEKNMKMIELHNQEYREGKHSFTMAMNAFGDMTSEEFR-QVMNGFQNRKPRKGKVFQEPLFYEAPRSVDWREKGYVTPVKNQGQCGSCWAFSATGALEGQMFRKTGRLISLSEQNLVDCSGPQGNEGCNGGLMDYAFQYVQDNGGLDSEESYPYEATEESCKYNPKYSVANDTGFVDIPKQEKALMKAVATVGPISVAIDAGHESFLFYKEGIY--FEPDCSSEDMDHGVLVVGYGFESTESDNNKYWLVKNSWGEEWGMGGYVKMAKDRRNHCGIASAASYPTV 333          
BLAST of Cathepsin F vs. Ensembl Human
Match: CTSL (cathepsin L [Source:HGNC Symbol;Acc:HGNC:2537])

HSP 1 Score: 197.208 bits (500), Expect = 1.919e-57
Identity = 124/306 (40.52%), Postives = 166/306 (54.25%), Query Frame = 2
Query:  692 FKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMN---RLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTI--DNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAF--AMQFYRGGISHPFSIFCNPNHLDHGVLIV--GFNSTTS-GEPFWIVKNSWGESWGENGYYLVYRG-SGVCGLNKMATTSVI 1576
            +K   N+LY   EE  +R  ++  N K   L N   R  K S    +  F D+T EEFR Q ++   +   R  ++ Q P   +  ++VDWR  G VT VKNQG CGSCWAFS TG +EGQ F KT RL+SLSEQ LVDC     + GCNGGL   A++ +Q  GGL+SE+SYPY A + +C  +         G   I   E  +    +  GPIS+ I+A   +  FY+ GI   F   C+   +DHGVL+V  GF ST S    +W+VKNSWGE WG  GY  + +     CG+   A+   +
Sbjct:   32 WKAMHNRLYGMNEEGWRR-AVWEKNMKMIELHNQEYREGKHSFTMAMNAFGDMTSEEFR-QVMNGFQNRKPRKGKVFQEPLFYEAPRSVDWREKGYVTPVKNQGQCGSCWAFSATGALEGQMFRKTGRLISLSEQNLVDCSGPQGNEGCNGGLMDYAFQYVQDNGGLDSEESYPYEATEESCKYNPKYSVANDTGFVDIPKQEKALMKAVATVGPISVAIDAGHESFLFYKEGIY--FEPDCSSEDMDHGVLVVGYGFESTESDNNKYWLVKNSWGEEWGMGGYVKMAKDRRNHCGIASAASYPTV 333          
BLAST of Cathepsin F vs. Ensembl Human
Match: CTSW (cathepsin W [Source:HGNC Symbol;Acc:HGNC:2546])

HSP 1 Score: 193.356 bits (490), Expect = 2.064e-55
Identity = 106/323 (32.82%), Postives = 158/323 (48.92%), Query Frame = 2
Query:  683 FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRS--QYLSPAYDLSKRPQRISQIPQDIKTAQAVDWRALG-GVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNAN--KGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGE-------------------PFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            F  F+  FN+ Y + EE A R  IF  N  +A  +   D G+A +GVT FSDLT+EEF     Y   A  +    + I     +     + DWR +   ++ +K+Q +C  CWA +  GN+E  W +     V +S Q+L+DC    +GC+GG    A+  +    GL SE  YP+        C   K +   +I     + ++E  +A + +  GPI++ IN   +Q YR G+       C+P  +DH VL+VGF S  S E                   P+WI+KNSWG  WGE GY+ ++RGS  CG+ K   T+ + 
Sbjct:   42 FKLFQIQFNRSYLSPEEHAHRLDIFAHNLAQAQRLQEEDLGTAEFGVTPFSDLTEEEFGQLYGYRRAAGGVPSMGREIRSEEPEESVPFSCDWRKVASAISPIKDQKNCNCCWAMAAAGNIETLWRISFWDFVDVSVQELLDCGRCGDGCHGGFVWDAFITVLNNSGLASEKDYPFQGKVRAHRCHPKKYQKVAWIQDFIMLQNNEHRIAQYLATYGPITVTINMKPLQLYRKGVIKATPTTCDPQLVDHSVLLVGFGSVKSEEGIWAETVSSQSQPQPPHPTPYWILKNSWGAQWGEKGYFRLHRGSNTCGITKFPLTARVQ 364          
BLAST of Cathepsin F vs. Ensembl Celegans
Match: tag-196 (pep chromosome:WBcel235:V:8594914:8597500:1 gene:WBGene00007055.1 transcript:F41E6.6.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:tag-196)

HSP 1 Score: 341.273 bits (874), Expect = 2.944e-111
Identity = 186/454 (40.97%), Postives = 259/454 (57.05%), Query Frame = 2
Query:  299 KDKDLQEVF-REAINKYNSRVNSIYSYAPYQVHSIRQQVVAGILYEFEVTLVGTNCRN-------ELKRSNQLLSECGLNENLAPKRAKLQIVSTPWLEPKHHFEVMSEIEELD-----LKLHDQLFVLGLPDGEVVSGLN------FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRS-----QYLSPAYDLSK---RPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            +D+ L E F R  + K+N + N  Y +   +   + +++  GI Y   VTLV T+C+        +  R    L +C +  +   KR    +     +      E    I++ D     L   D + V  L   +++   +      F  F     K Y N+ E  KRF++F  N K    + + ++G+AVYG T+FSD+T  EF+      Q+  P Y + +       ++   +D+   ++ DWR  G VT VKNQG+CGSCWAFSTTGNVEG WF+   +LVSLSEQ+LVDCD++D GCNGGLPS AYK I +MGGLE ED+YPY+     C L +  IAVYINGS  +   E  M  W    GPISIG+NA  +QFYR G+ HPF IFC P  L+HGVLIVG+      +P+WIVKNSWG +WGE GY+ +YRG  VCG+ +MAT++++N
Sbjct:   28 EDQLLYEKFARHGVEKFNQQSNDAYKWELDRTWEVERKLSGGIHYSIFVTLVKTDCKKGQTDVEGKKCRKTDTLKKCQVEISRRVKRHGYGLKDIVHIRNCEE-EFTRNIDKFDHRKIQLTHDDSITVQELRKAKIIRPRDYVIWNSFLDFVDRHEKKYTNKREVLKRFRVFKKNAKVIRELQKNEQGTAVYGFTKFSDMTTMEFKKIMLPYQWEQPVYPMEQANFEKHDVTINEEDL--PESFDWREKGAVTQVKNQGNCGSCWAFSTTGNVEGAWFIAKNKLVSLSEQELVDCDSMDQGCNGGLPSNAYKEIIRMGGLEPEDAYPYDGRGETCHLVRKDIAVYINGSVELPHDEVEMQKWLVTKGPISIGLNANTLQFYRHGVVHPFKIFCEPFMLNHGVLIVGYGKDGR-KPYWIVKNSWGPNWGEAGYFKLYRGKNVCGVQEMATSALVN 477          
BLAST of Cathepsin F vs. Ensembl Celegans
Match: tag-196 (pep chromosome:WBcel235:V:8594914:8597469:1 gene:WBGene00007055.1 transcript:F41E6.6.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:tag-196)

HSP 1 Score: 341.273 bits (874), Expect = 2.944e-111
Identity = 186/454 (40.97%), Postives = 259/454 (57.05%), Query Frame = 2
Query:  299 KDKDLQEVF-REAINKYNSRVNSIYSYAPYQVHSIRQQVVAGILYEFEVTLVGTNCRN-------ELKRSNQLLSECGLNENLAPKRAKLQIVSTPWLEPKHHFEVMSEIEELD-----LKLHDQLFVLGLPDGEVVSGLN------FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRS-----QYLSPAYDLSK---RPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            +D+ L E F R  + K+N + N  Y +   +   + +++  GI Y   VTLV T+C+        +  R    L +C +  +   KR    +     +      E    I++ D     L   D + V  L   +++   +      F  F     K Y N+ E  KRF++F  N K    + + ++G+AVYG T+FSD+T  EF+      Q+  P Y + +       ++   +D+   ++ DWR  G VT VKNQG+CGSCWAFSTTGNVEG WF+   +LVSLSEQ+LVDCD++D GCNGGLPS AYK I +MGGLE ED+YPY+     C L +  IAVYINGS  +   E  M  W    GPISIG+NA  +QFYR G+ HPF IFC P  L+HGVLIVG+      +P+WIVKNSWG +WGE GY+ +YRG  VCG+ +MAT++++N
Sbjct:   28 EDQLLYEKFARHGVEKFNQQSNDAYKWELDRTWEVERKLSGGIHYSIFVTLVKTDCKKGQTDVEGKKCRKTDTLKKCQVEISRRVKRHGYGLKDIVHIRNCEE-EFTRNIDKFDHRKIQLTHDDSITVQELRKAKIIRPRDYVIWNSFLDFVDRHEKKYTNKREVLKRFRVFKKNAKVIRELQKNEQGTAVYGFTKFSDMTTMEFKKIMLPYQWEQPVYPMEQANFEKHDVTINEEDL--PESFDWREKGAVTQVKNQGNCGSCWAFSTTGNVEGAWFIAKNKLVSLSEQELVDCDSMDQGCNGGLPSNAYKEIIRMGGLEPEDAYPYDGRGETCHLVRKDIAVYINGSVELPHDEVEMQKWLVTKGPISIGLNANTLQFYRHGVVHPFKIFCEPFMLNHGVLIVGYGKDGR-KPYWIVKNSWGPNWGEAGYFKLYRGKNVCGVQEMATSALVN 477          
BLAST of Cathepsin F vs. Ensembl Celegans
Match: R09F10.1 (pep chromosome:WBcel235:X:8323592:8325046:1 gene:WBGene00019986.1 transcript:R09F10.1.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:R09F10.1)

HSP 1 Score: 195.667 bits (496), Expect = 1.035e-56
Identity = 115/306 (37.58%), Postives = 167/306 (54.58%), Query Frame = 2
Query:  683 FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLS---PAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGN-CALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAF-AMQFYRGGISHPFSIFCNPNHLD-HGVLIVGFNSTTSGE-PFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            F+ F   F++ Y + EE   R++IF  N  +       + G  +  V +F+D TDEE +          YD        S +   +    ++DWR  G +T +KNQG CGSCWAF+T  +VE Q  +K  +LVSLSEQ++VDCD  +NGC+GG    A K +++  GLESE  YPY+A K + C L ++   V+I+    + ++E  +ANW    GP++ G+N   AM  YR GI +P    C    +  H + I+G+     GE  +WIVKNSWG SWG +GY+ + RG   CGL       +IN
Sbjct:   82 FNDFILKFDRKYTSVEEFEYRYQIFLRNVIEFEAEEERNLGLDL-DVNEFTDWTDEELQKMVQENKYTKYDFDTPKFEGSYLETGVIRPASIDWREQGKLTPIKNQGQCGSCWAFATVASVEAQNAIKKGKLVSLSEQEMVDCDGRNNGCSGGYRPYAMKFVKE-NGLESEKEYPYSALKHDQCFLKENDTRVFIDDFRMLSNNEEDIANWVGTKGPVTFGMNVVKAMYSYRSGIFNPSVEDCTEKSMGAHALTIIGYGG--EGESAYWIVKNSWGTSWGASGYFRLARGVNSCGLANTVVAPIIN 383          
BLAST of Cathepsin F vs. Ensembl Celegans
Match: R07E3.1 (pep chromosome:WBcel235:X:10339226:10341095:-1 gene:WBGene00011102.1 transcript:R07E3.1b.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:R07E3.1)

HSP 1 Score: 179.874 bits (455), Expect = 3.713e-51
Identity = 104/303 (34.32%), Postives = 157/303 (51.82%), Query Frame = 2
Query:  689 SFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMN-RLDKGSAVYGVTQFSDLTDEEFRSQYLSPAY--DLSKRPQRISQIPQDIKTAQA---------VDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGN-CALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAF-AMQFYRGGISHPFSIFCNPNHLD-HGVLIVGFNSTTSGEPFWIVKNSWGESWG-ENGYYLVYRGSGVCGL 1549
            ++ + F+K Y   +E  KR   +++  +     N + + GSA YG    SD TDEEF    L  ++   L K  + I  IP+ +   +           DWR    +T VK QG CGSCWAF++T  VE  W +      +LSEQ L+DCD +DN C+GG   +A++ I +  GL +    PY A++ N CA++       I  +  +   E  + NW    GP++IG+     M+ Y+GG+  P    C    +  H +LI G+ ++ +GE +WIVKNSWG +WG E+GY    RG   CG+
Sbjct:   38 AYTEKFDKSYATSQESLKRLNAYYNTDENIANWNIQNEHGSAEYGHNDMSDWTDEEFEKTLLPKSFYKRLHKEAEFIEPIPESLTAKKGESSSPFPDFFDWRDKNVITPVKAQGQCGSCWAFASTATVEAAWAIAHGEKRNLSEQTLLDCDLVDNACDGGDEDKAFRYIHR-NGLANAVDLPYVAHRQNGCAVNDHWNTTRIKAAYFLHHDEDSIINWLVNFGPVNIGMAVIQPMRAYKGGVFTPSEYACKNEVIGLHALLITGYGTSKTGEKYWIVKNSWGNTWGVEHGYIYFARGINACGI 339          
BLAST of Cathepsin F vs. Ensembl Celegans
Match: R07E3.1 (pep chromosome:WBcel235:X:10339226:10340968:-1 gene:WBGene00011102.1 transcript:R07E3.1b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:R07E3.1)

HSP 1 Score: 179.874 bits (455), Expect = 3.713e-51
Identity = 104/303 (34.32%), Postives = 157/303 (51.82%), Query Frame = 2
Query:  689 SFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMN-RLDKGSAVYGVTQFSDLTDEEFRSQYLSPAY--DLSKRPQRISQIPQDIKTAQA---------VDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGN-CALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAF-AMQFYRGGISHPFSIFCNPNHLD-HGVLIVGFNSTTSGEPFWIVKNSWGESWG-ENGYYLVYRGSGVCGL 1549
            ++ + F+K Y   +E  KR   +++  +     N + + GSA YG    SD TDEEF    L  ++   L K  + I  IP+ +   +           DWR    +T VK QG CGSCWAF++T  VE  W +      +LSEQ L+DCD +DN C+GG   +A++ I +  GL +    PY A++ N CA++       I  +  +   E  + NW    GP++IG+     M+ Y+GG+  P    C    +  H +LI G+ ++ +GE +WIVKNSWG +WG E+GY    RG   CG+
Sbjct:   38 AYTEKFDKSYATSQESLKRLNAYYNTDENIANWNIQNEHGSAEYGHNDMSDWTDEEFEKTLLPKSFYKRLHKEAEFIEPIPESLTAKKGESSSPFPDFFDWRDKNVITPVKAQGQCGSCWAFASTATVEAAWAIAHGEKRNLSEQTLLDCDLVDNACDGGDEDKAFRYIHR-NGLANAVDLPYVAHRQNGCAVNDHWNTTRIKAAYFLHHDEDSIINWLVNFGPVNIGMAVIQPMRAYKGGVFTPSEYACKNEVIGLHALLITGYGTSKTGEKYWIVKNSWGNTWGVEHGYIYFARGINACGI 339          
BLAST of Cathepsin F vs. Ensembl Fly
Match: CG12163 (gene:FBgn0260462 transcript:FBtr0078822)

HSP 1 Score: 285.034 bits (728), Expect = 2.002e-89
Identity = 166/427 (38.88%), Postives = 229/427 (53.63%), Query Frame = 2
Query:  374 YAPYQVHSIRQQVVAGILYEFEVTLVGTN-----CRNEL-------KRSNQLLSECGLNENLAPKRAKLQIVSTPWLEPKHHFEVMSEIEELDLKLHDQLFVLGLPDGEVVSGLNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKR--------PQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASI-DSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGE-----PFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVI 1576
            Y   +V+S  +QV +GIL   +  L+  +     C  ++       K  +++  +C  N+ +   R      S  W E K H            K H   F       + V  L F  F+  F + Y +  E   R +IF  N K    +N  + GSA YG+T+F+D+T  E++ +      D +K         P    ++P++       DWR    VT VKNQGSCGSCWAFS TGN+EG + +KT  L   SEQ+L+DCDT D+ CNGGL   AYKAI+ +GGLE E  YPY A K  C  +++   V + G   +   +E+ M  W   NGPISIGINA AMQFYRGG+SHP+   C+  +LDHGVL+VG+  +         P+WIVKNSWG  WGE GYY VYRG   CG+++MAT++V+
Sbjct:   78 YKIVKVYSASRQVDSGILTRIDADLIDGSEEQHRCIVDIWTKVWVRKDEHEITFKC-RNQPVVQAR---HTRSVEWAEKKTH------------KKHSHRF-------DKVDHL-FYKFQVRFGRRYVSTAERQMRLRIFRQNLKTIEELNANEMGSAKYGITEFADMTSSEYKERTGLWQRDEAKATGGSAAVVPAYHGELPKEF------DWRQKDAVTQVKNQGSCGSCWAFSVTGNIEGLYAVKTGELKEFSEQELLDCDTTDSACNGGLMDNAYKAIKDIGGLEYEAEYPYKAKKNQCHFNRTLSHVQVAGFVDLPKGNETAMQEWLLANGPISIGINANAMQFYRGGVSHPWKALCSKKNLDHGVLVVGYGVSDYPNFHKTLPYWIVKNSWGPRWGEQGYYRVYRGDNTCGVSEMATSAVL 474          
BLAST of Cathepsin F vs. Ensembl Fly
Match: CG12163 (gene:FBgn0260462 transcript:FBtr0078823)

HSP 1 Score: 285.034 bits (728), Expect = 6.831e-88
Identity = 142/312 (45.51%), Postives = 189/312 (60.58%), Query Frame = 2
Query:  683 FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKR--------PQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASI-DSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGE-----PFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVI 1576
            F  F+  F + Y +  E   R +IF  N K    +N  + GSA YG+T+F+D+T  E++ +      D +K         P    ++P++       DWR    VT VKNQGSCGSCWAFS TGN+EG + +KT  L   SEQ+L+DCDT D+ CNGGL   AYKAI+ +GGLE E  YPY A K  C  +++   V + G   +   +E+ M  W   NGPISIGINA AMQFYRGG+SHP+   C+  +LDHGVL+VG+  +         P+WIVKNSWG  WGE GYY VYRG   CG+++MAT++V+
Sbjct:  308 FYKFQVRFGRRYVSTAERQMRLRIFRQNLKTIEELNANEMGSAKYGITEFADMTSSEYKERTGLWQRDEAKATGGSAAVVPAYHGELPKEF------DWRQKDAVTQVKNQGSCGSCWAFSVTGNIEGLYAVKTGELKEFSEQELLDCDTTDSACNGGLMDNAYKAIKDIGGLEYEAEYPYKAKKNQCHFNRTLSHVQVAGFVDLPKGNETAMQEWLLANGPISIGINANAMQFYRGGVSHPWKALCSKKNLDHGVLVVGYGVSDYPNFHKTLPYWIVKNSWGPRWGEQGYYRVYRGDNTCGVSEMATSAVL 613          
BLAST of Cathepsin F vs. Ensembl Fly
Match: Cp1 (gene:FBgn0013770 transcript:FBtr0087592)

HSP 1 Score: 216.083 bits (549), Expect = 8.435e-65
Identity = 125/313 (39.94%), Postives = 175/313 (55.91%), Query Frame = 2
Query:  689 SFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMN-RLDKGSAVY--GVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQI--------PQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTI--DNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASI-DSSESFMANWCSVNGPISIGINAF--AMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRG-SGVCGLNKMATTSVI 1576
            +FK    K YQ+E EE  R KIF+ N  K    N R  +G   +   V +++DL   EFR       Y L K+ +   +         P  +   ++VDWR  G VTAVK+QG CGSCWAFS+TG +EGQ F K+  LVSLSEQ LVDC T   +NGCNGGL   A++ I+  GG+++E SYPY A   +C  +K  +     G   I    E  MA   +  GP+S+ I+A   + QFY  G+ +     C+  +LDHGVL+VGF +  SGE +W+VKNSWG +WG+ G+  + R     CG+   ++  ++
Sbjct:   31 TFKLEHRKNYQDETEERFRLKIFNENKHKIAKHNQRFAEGKVSFKLAVNKYADLLHHEFRQLMNGFNYTLHKQLRAADESFKGVTFISPAHVTLPKSVDWRTKGAVTAVKDQGHCGSCWAFSSTGALEGQHFRKSGVLVSLSEQNLVDCSTKYGNNGCNGGLMDNAFRYIKDNGGIDTEKSYPYEAIDDSCHFNKGTVGATDRGFTDIPQGDEKKMAEAVATVGPVSVAIDASHESFQFYSEGVYNEPQ--CDAQNLDHGVLVVGFGTDESGEDYWLVKNSWGTTWGDKGFIKMLRNKENQCGIASASSYPLV 341          
BLAST of Cathepsin F vs. Ensembl Fly
Match: Cp1 (gene:FBgn0013770 transcript:FBtr0087593)

HSP 1 Score: 216.853 bits (551), Expect = 1.087e-64
Identity = 125/313 (39.94%), Postives = 175/313 (55.91%), Query Frame = 2
Query:  689 SFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMN-RLDKGSAVY--GVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQI--------PQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTI--DNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASI-DSSESFMANWCSVNGPISIGINAF--AMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRG-SGVCGLNKMATTSVI 1576
            +FK    K YQ+E EE  R KIF+ N  K    N R  +G   +   V +++DL   EFR       Y L K+ +   +         P  +   ++VDWR  G VTAVK+QG CGSCWAFS+TG +EGQ F K+  LVSLSEQ LVDC T   +NGCNGGL   A++ I+  GG+++E SYPY A   +C  +K  +     G   I    E  MA   +  GP+S+ I+A   + QFY  G+ +     C+  +LDHGVL+VGF +  SGE +W+VKNSWG +WG+ G+  + R     CG+   ++  ++
Sbjct:   61 TFKLEHRKNYQDETEERFRLKIFNENKHKIAKHNQRFAEGKVSFKLAVNKYADLLHHEFRQLMNGFNYTLHKQLRAADESFKGVTFISPAHVTLPKSVDWRTKGAVTAVKDQGHCGSCWAFSSTGALEGQHFRKSGVLVSLSEQNLVDCSTKYGNNGCNGGLMDNAFRYIKDNGGIDTEKSYPYEAIDDSCHFNKGTVGATDRGFTDIPQGDEKKMAEAVATVGPVSVAIDASHESFQFYSEGVYNEPQ--CDAQNLDHGVLVVGFGTDESGEDYWLVKNSWGTTWGDKGFIKMLRNKENQCGIASASSYPLV 371          
BLAST of Cathepsin F vs. Ensembl Fly
Match: Cp1 (gene:FBgn0013770 transcript:FBtr0339955)

HSP 1 Score: 213.386 bits (542), Expect = 9.342e-64
Identity = 123/306 (40.20%), Postives = 172/306 (56.21%), Query Frame = 2
Query:  710 KLYQNEEEEAKRFKIFHSNYKKALLMN-RLDKGSAVY--GVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQI--------PQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTI--DNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASI-DSSESFMANWCSVNGPISIGINAF--AMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGS-GVCGLNKMATTSVI 1576
            K YQ+E EE  R KIF+ N  K    N R  +G   +   V +++DL   EFR       Y L K+ +   +         P  +   ++VDWR  G VTAVK+QG CGSCWAFS+TG +EGQ F K+  LVSLSEQ LVDC T   +NGCNGGL   A++ I+  GG+++E SYPY A   +C  +K  +     G   I    E  MA   +  GP+S+ I+A   + QFY  G+ +     C+  +LDHGVL+VGF +  SGE +W+VKNSWG +WG+ G+  + R     CG+   ++  ++
Sbjct:   35 KNYQDETEERFRLKIFNENKHKIAKHNQRFAEGKVSFKLAVNKYADLLHHEFRQLMNGFNYTLHKQLRAADESFKGVTFISPAHVTLPKSVDWRTKGAVTAVKDQGHCGSCWAFSSTGALEGQHFRKSGVLVSLSEQNLVDCSTKYGNNGCNGGLMDNAFRYIKDNGGIDTEKSYPYEAIDDSCHFNKGTVGATDRGFTDIPQGDEKKMAEAVATVGPVSVAIDASHESFQFYSEGVYNEPQ--CDAQNLDHGVLVVGFGTDESGEDYWLVKNSWGTTWGDKGFIKMLRNKENQCGIASASSYPLV 338          
BLAST of Cathepsin F vs. Ensembl Zebrafish
Match: ctsf (cathepsin F [Source:ZFIN;Acc:ZDB-GENE-030131-9831])

HSP 1 Score: 347.436 bits (890), Expect = 1.705e-113
Identity = 183/439 (41.69%), Postives = 259/439 (59.00%), Query Frame = 2
Query:  302 DKDLQEVFREAINKYNSRVNSIYSYAPYQVHSIRQQVVAGILYEFEVTLVGTNCRNELKRSNQLLSECGLNENLAPKRAKLQIV-----STPW------LEPKHHFEVMSEIEELDLKLHDQLFVLGLPDGEVVSGLN-FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYD-LSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            D  + +  + A  +YN   N+++     ++ S  +Q+V G+ Y   V +  T C    K++ +L +    +    P + K ++        PW      L+ K    V+ E++    K+         P  E V  L  F +F   +N+ Y ++EE  KR +IF  N K A  +  L++GSA YG+T+FSDLT++EFR  YL+P     S + +    IP         DWR  G V+ VKNQG CGSCWAFS TGN+EGQWF KT +L+SLSEQ+LVDCD +D  C GGLPS AY+AI+ +GGLE+E  Y Y  +K +C     K+A YIN S  +   E  +A + + NGP+S  +NAFAMQFYR G+SHP  IFCNP  +DH VL+VGF    +G PFW +KNSWGE +GE GYY +YRGSG+CG++KM +++++N
Sbjct:   44 DPGVMKAVKFAEERYNMGSNAMHIRRVSKILSASKQLVKGVRYSIMVEIGSTQC----KKAAELSTADTCDFFPEPHKQKTEVCLFEVWDIPWENKSTLLKQKCQPAVVKELK----KVAAVPLTHSKPMKESVELLTMFKNFMITYNRTYSSQEEAEKRLRIFQQNMKTAQTLQSLEQGSAEYGITKFSDLTEDEFRMMYLNPMLSQWSLKKEMKPAIPASAPAPDTWDWRDHGAVSPVKNQGMCGSCWAFSVTGNIEGQWFKKTGQLLSLSEQELVDCDKLDQACGGGLPSNAYEAIENLGGLETETDYSYTGHKQSCDFSTGKVAAYINSSVELPKDEKEIAAFLAENGPVSAALNAFAMQFYRKGVSHPLKIFCNPRMIDHAVLLVGFGQ-RNGVPFWAIKNSWGEDYGEQGYYYLYRGSGLCGIHKMCSSAIVN 473          
BLAST of Cathepsin F vs. Ensembl Zebrafish
Match: ctsf (cathepsin F [Source:ZFIN;Acc:ZDB-GENE-030131-9831])

HSP 1 Score: 347.436 bits (890), Expect = 1.705e-113
Identity = 183/439 (41.69%), Postives = 259/439 (59.00%), Query Frame = 2
Query:  302 DKDLQEVFREAINKYNSRVNSIYSYAPYQVHSIRQQVVAGILYEFEVTLVGTNCRNELKRSNQLLSECGLNENLAPKRAKLQIV-----STPW------LEPKHHFEVMSEIEELDLKLHDQLFVLGLPDGEVVSGLN-FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYD-LSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            D  + +  + A  +YN   N+++     ++ S  +Q+V G+ Y   V +  T C    K++ +L +    +    P + K ++        PW      L+ K    V+ E++    K+         P  E V  L  F +F   +N+ Y ++EE  KR +IF  N K A  +  L++GSA YG+T+FSDLT++EFR  YL+P     S + +    IP         DWR  G V+ VKNQG CGSCWAFS TGN+EGQWF KT +L+SLSEQ+LVDCD +D  C GGLPS AY+AI+ +GGLE+E  Y Y  +K +C     K+A YIN S  +   E  +A + + NGP+S  +NAFAMQFYR G+SHP  IFCNP  +DH VL+VGF    +G PFW +KNSWGE +GE GYY +YRGSG+CG++KM +++++N
Sbjct:   44 DPGVMKAVKFAEERYNMGSNAMHIRRVSKILSASKQLVKGVRYSIMVEIGSTQC----KKAAELSTADTCDFFPEPHKQKTEVCLFEVWDIPWENKSTLLKQKCQPAVVKELK----KVAAVPLTHSKPMKESVELLTMFKNFMITYNRTYSSQEEAEKRLRIFQQNMKTAQTLQSLEQGSAEYGITKFSDLTEDEFRMMYLNPMLSQWSLKKEMKPAIPASAPAPDTWDWRDHGAVSPVKNQGMCGSCWAFSVTGNIEGQWFKKTGQLLSLSEQELVDCDKLDQACGGGLPSNAYEAIENLGGLETETDYSYTGHKQSCDFSTGKVAAYINSSVELPKDEKEIAAFLAENGPVSAALNAFAMQFYRKGVSHPLKIFCNPRMIDHAVLLVGFGQ-RNGVPFWAIKNSWGEDYGEQGYYYLYRGSGLCGIHKMCSSAIVN 473          
BLAST of Cathepsin F vs. Ensembl Zebrafish
Match: ctsl.1 (cathepsin L.1 [Source:ZFIN;Acc:ZDB-GENE-040718-61])

HSP 1 Score: 203.371 bits (516), Expect = 7.851e-60
Identity = 124/314 (39.49%), Postives = 179/314 (57.01%), Query Frame = 2
Query:  677 LNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRL-DKGSAVY--GVTQFSDLTDEEFRSQYL-----SPAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTI--DNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDS-SESFMANWCSVNGPISIGINA--FAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRG-SGVCGLNKMATTSVI 1576
            + F ++K  F K Y++ EEE+ R   + +N K  L+ N + D+G   Y  G+T F+D+++EE+R         S     ++      ++ +       VDWR  G VT +K+Q  CGSCWAFS TG++EGQ F KT +LVSLSEQQLVDC     + GC+GGL  QA++ I+   GL++EDSYPY A  G C  + S +     G   I S  ES +    +  GPIS+ I+A   + Q Y  G+ +     C+ + LDHGVL VG+ S ++G+ +WIVKNSWG  WG  GY L+ R  S  CG+   A+  ++
Sbjct:   24 MEFHAWKLKFGKSYRSAEEESHRQLTWLTNRKLVLVHNMMADQGLKSYRLGMTYFADMSNEEYRQLVFRGCLGSMNNTKARGGSTFFRLRKAAVVPDTVDWRDKGYVTDIKDQKQCGSCWAFSATGSLEGQTFRKTGKLVSLSEQQLVDCSGSYGNYGCDGGLMDQAFQYIEANKGLDTEDSYPYEAQDGECRFNPSTVGASCTGYVDIASGDESALQEAVATIGPISVAIDAGHSSFQLYSSGVYNEPD--CSSSELDHGVLAVGYGS-SNGDDYWIVKNSWGLDWGVQGYILMSRNKSNQCGIATAASYPLV 334          
BLAST of Cathepsin F vs. Ensembl Zebrafish
Match: ctsl.1 (cathepsin L.1 [Source:ZFIN;Acc:ZDB-GENE-040718-61])

HSP 1 Score: 203.371 bits (516), Expect = 7.851e-60
Identity = 124/314 (39.49%), Postives = 179/314 (57.01%), Query Frame = 2
Query:  677 LNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRL-DKGSAVY--GVTQFSDLTDEEFRSQYL-----SPAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTI--DNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDS-SESFMANWCSVNGPISIGINA--FAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRG-SGVCGLNKMATTSVI 1576
            + F ++K  F K Y++ EEE+ R   + +N K  L+ N + D+G   Y  G+T F+D+++EE+R         S     ++      ++ +       VDWR  G VT +K+Q  CGSCWAFS TG++EGQ F KT +LVSLSEQQLVDC     + GC+GGL  QA++ I+   GL++EDSYPY A  G C  + S +     G   I S  ES +    +  GPIS+ I+A   + Q Y  G+ +     C+ + LDHGVL VG+ S ++G+ +WIVKNSWG  WG  GY L+ R  S  CG+   A+  ++
Sbjct:   24 MEFHAWKLKFGKSYRSAEEESHRQLTWLTNRKLVLVHNMMADQGLKSYRLGMTYFADMSNEEYRQLVFRGCLGSMNNTKARGGSTFFRLRKAAVVPDTVDWRDKGYVTDIKDQKQCGSCWAFSATGSLEGQTFRKTGKLVSLSEQQLVDCSGSYGNYGCDGGLMDQAFQYIEANKGLDTEDSYPYEAQDGECRFNPSTVGASCTGYVDIASGDESALQEAVATIGPISVAIDAGHSSFQLYSSGVYNEPD--CSSSELDHGVLAVGYGS-SNGDDYWIVKNSWGLDWGVQGYILMSRNKSNQCGIATAASYPLV 334          
BLAST of Cathepsin F vs. Ensembl Zebrafish
Match: ctsl.1 (cathepsin L.1 [Source:ZFIN;Acc:ZDB-GENE-040718-61])

HSP 1 Score: 203.371 bits (516), Expect = 7.851e-60
Identity = 124/314 (39.49%), Postives = 179/314 (57.01%), Query Frame = 2
Query:  677 LNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRL-DKGSAVY--GVTQFSDLTDEEFRSQYL-----SPAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTI--DNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDS-SESFMANWCSVNGPISIGINA--FAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRG-SGVCGLNKMATTSVI 1576
            + F ++K  F K Y++ EEE+ R   + +N K  L+ N + D+G   Y  G+T F+D+++EE+R         S     ++      ++ +       VDWR  G VT +K+Q  CGSCWAFS TG++EGQ F KT +LVSLSEQQLVDC     + GC+GGL  QA++ I+   GL++EDSYPY A  G C  + S +     G   I S  ES +    +  GPIS+ I+A   + Q Y  G+ +     C+ + LDHGVL VG+ S ++G+ +WIVKNSWG  WG  GY L+ R  S  CG+   A+  ++
Sbjct:   24 MEFHAWKLKFGKSYRSAEEESHRQLTWLTNRKLVLVHNMMADQGLKSYRLGMTYFADMSNEEYRQLVFRGCLGSMNNTKARGGSTFFRLRKAAVVPDTVDWRDKGYVTDIKDQKQCGSCWAFSATGSLEGQTFRKTGKLVSLSEQQLVDCSGSYGNYGCDGGLMDQAFQYIEANKGLDTEDSYPYEAQDGECRFNPSTVGASCTGYVDIASGDESALQEAVATIGPISVAIDAGHSSFQLYSSGVYNEPD--CSSSELDHGVLAVGYGS-SNGDDYWIVKNSWGLDWGVQGYILMSRNKSNQCGIATAASYPLV 334          
BLAST of Cathepsin F vs. Ensembl Xenopus
Match: klhl31 (kelch like family member 31 [Source:Xenbase;Acc:XB-GENE-966130])

HSP 1 Score: 377.096 bits (967), Expect = 3.540e-125
Identity = 193/444 (43.47%), Postives = 267/444 (60.14%), Query Frame = 2
Query:  308 DLQEVFREAINKYNSRVNSIYSYAPYQVHSIRQQVVAGILYEFEVTLVGTNCRNELKRSNQLLSEC--GLNENLAPKRAKLQIVSTPWLEPKHHFEVMSEIEELDLKLHDQLFVLGLPDGEVVSGLN----------------FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRP--QRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            +L++V   A+ ++N   N    Y   +V   R++VV+G+ Y+  + L  T C+   K  + LL  C   ++  L   +    I+  PW         M+E + L      ++F++ L D E     N                F  F   +NK Y ++EE A+R +IF  N KKA ++  +D+G+A YGVT++SDLT++EFRS YL+P   LS +P  Q    I  ++      DWR  G VT VKNQG CGSCWAFS  GN+EGQWFLK   LVSLSEQ+LVDCD +D+ C GGLPS AY+AI+++GG+E+E  Y Y  +K  C+   SK++ YIN S  I   E+ +A W + NGPISI +NAFAMQFYR GISHPF I CNP  +DH VL+VG+    +G PFW +KNSWG  WGE GYY +YRG+G CG+N M +++V++
Sbjct:   20 ELKQVAEVAVREFNRGNNGEEIYRQIRVTQARKKVVSGLNYDLTLILGRTQCK---KGQDFLLGSCLQTMSPPLMGVKCTFSILIVPW---------MNETKILHQMCSQEIFIVELTDTETSQKQNVPSSELEDEMLKTLTLFKDFVTTYNKKYSDQEEAARRLQIFSQNLKKAQMIQEMDQGTAEYGVTKYSDLTEDEFRSLYLNPL--LSSKPLYQMKKAIVPNMSAPDQWDWRDHGAVTEVKNQGMCGSCWAFSVIGNIEGQWFLKKGSLVSLSEQELVDCDGVDHACAGGLPSNAYEAIEKLGGIETEQEYSYEGHKNTCSFSTSKVSAYINSSVEIPKDENEIAAWLAQNGPISIALNAFAMQFYRKGISHPFRILCNPWMIDHAVLLVGYGE-RNGTPFWAIKNSWGTDWGEQGYYYLYRGTGACGMNTMCSSAVVD 448          
BLAST of Cathepsin F vs. Ensembl Xenopus
Match: klhl31 (kelch like family member 31 [Source:Xenbase;Acc:XB-GENE-966130])

HSP 1 Score: 376.711 bits (966), Expect = 8.024e-125
Identity = 193/444 (43.47%), Postives = 267/444 (60.14%), Query Frame = 2
Query:  308 DLQEVFREAINKYNSRVNSIYSYAPYQVHSIRQQVVAGILYEFEVTLVGTNCRNELKRSNQLLSEC--GLNENLAPKRAKLQIVSTPWLEPKHHFEVMSEIEELDLKLHDQLFVLGLPDGEVVSGLN----------------FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRP--QRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            +L++V   A+ ++N   N    Y   +V   R++VV+G+ Y+  + L  T C+   K  + LL  C   ++  L   +    I+  PW         M+E + L      ++F++ L D E     N                F  F   +NK Y ++EE A+R +IF  N KKA ++  +D+G+A YGVT++SDLT++EFRS YL+P   LS +P  Q    I  ++      DWR  G VT VKNQG CGSCWAFS  GN+EGQWFLK   LVSLSEQ+LVDCD +D+ C GGLPS AY+AI+++GG+E+E  Y Y  +K  C+   SK++ YIN S  I   E+ +A W + NGPISI +NAFAMQFYR GISHPF I CNP  +DH VL+VG+    +G PFW +KNSWG  WGE GYY +YRG+G CG+N M +++V++
Sbjct:   32 ELKQVAEVAVREFNRGNNGEEIYRQIRVTQARKKVVSGLNYDLTLILGRTQCK---KGQDFLLGSCLQTMSPPLMGVKCTFSILIVPW---------MNETKILHQMCSQEIFIVELTDTETSQKQNVPSSELEDEMLKTLTLFKDFVTTYNKKYSDQEEAARRLQIFSQNLKKAQMIQEMDQGTAEYGVTKYSDLTEDEFRSLYLNPL--LSSKPLYQMKKAIVPNMSAPDQWDWRDHGAVTEVKNQGMCGSCWAFSVIGNIEGQWFLKKGSLVSLSEQELVDCDGVDHACAGGLPSNAYEAIEKLGGIETEQEYSYEGHKNTCSFSTSKVSAYINSSVEIPKDENEIAAWLAQNGPISIALNAFAMQFYRKGISHPFRILCNPWMIDHAVLLVGYGE-RNGTPFWAIKNSWGTDWGEQGYYYLYRGTGACGMNTMCSSAVVD 460          
BLAST of Cathepsin F vs. Ensembl Xenopus
Match: klhl31 (kelch like family member 31 [Source:Xenbase;Acc:XB-GENE-966130])

HSP 1 Score: 339.347 bits (869), Expect = 1.064e-112
Identity = 156/285 (54.74%), Postives = 202/285 (70.88%), Query Frame = 2
Query:  731 EEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRP--QRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            E A+R +IF  N KKA ++  +D+G+A YGVT++SDLT++EFRS YL+P   LS +P  Q    I  ++      DWR  G VT VKNQG CGSCWAFS  GN+EGQWFLK   LVSLSEQ+LVDCD +D+ C GGLPS AY+AI+++GG+E+E  Y Y  +K  C+   SK++ YIN S  I   E+ +A W + NGPISI +NAFAMQFYR GISHPF I CNP  +DH VL+VG+    +G PFW +KNSWG  WGE GYY +YRG+G CG+N M +++V++
Sbjct:   14 EAARRLQIFSQNLKKAQMIQEMDQGTAEYGVTKYSDLTEDEFRSLYLNPL--LSSKPLYQMKKAIVPNMSAPDQWDWRDHGAVTEVKNQGMCGSCWAFSVIGNIEGQWFLKKGSLVSLSEQELVDCDGVDHACAGGLPSNAYEAIEKLGGIETEQEYSYEGHKNTCSFSTSKVSAYINSSVEIPKDENEIAAWLAQNGPISIALNAFAMQFYRKGISHPFRILCNPWMIDHAVLLVGYGE-RNGTPFWAIKNSWGTDWGEQGYYYLYRGTGACGMNTMCSSAVVD 295          
BLAST of Cathepsin F vs. Ensembl Xenopus
Match: klhl31 (kelch like family member 31 [Source:Xenbase;Acc:XB-GENE-966130])

HSP 1 Score: 333.569 bits (854), Expect = 2.701e-110
Identity = 154/281 (54.80%), Postives = 199/281 (70.82%), Query Frame = 2
Query:  743 RFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRP--QRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            R +IF  N KKA ++  +D+G+A YGVT++SDLT++EFRS YL+P   LS +P  Q    I  ++      DWR  G VT VKNQG CGSCWAFS  GN+EGQWFLK   LVSLSEQ+LVDCD +D+ C GGLPS AY+AI+++GG+E+E  Y Y  +K  C+   SK++ YIN S  I   E+ +A W + NGPISI +NAFAMQFYR GISHPF I CNP  +DH VL+VG+    +G PFW +KNSWG  WGE GYY +YRG+G CG+N M +++V++
Sbjct:   27 RLQIFSQNLKKAQMIQEMDQGTAEYGVTKYSDLTEDEFRSLYLNPL--LSSKPLYQMKKAIVPNMSAPDQWDWRDHGAVTEVKNQGMCGSCWAFSVIGNIEGQWFLKKGSLVSLSEQELVDCDGVDHACAGGLPSNAYEAIEKLGGIETEQEYSYEGHKNTCSFSTSKVSAYINSSVEIPKDENEIAAWLAQNGPISIALNAFAMQFYRKGISHPFRILCNPWMIDHAVLLVGYGE-RNGTPFWAIKNSWGTDWGEQGYYYLYRGTGACGMNTMCSSAVVD 304          
BLAST of Cathepsin F vs. Ensembl Xenopus
Match: klhl31 (kelch like family member 31 [Source:Xenbase;Acc:XB-GENE-966130])

HSP 1 Score: 337.035 bits (863), Expect = 1.499e-109
Identity = 180/444 (40.54%), Postives = 253/444 (56.98%), Query Frame = 2
Query:  308 DLQEVFREAINKYNSRVNSIYSYAPYQVHSIRQQVVAGILYEFEVTLVGTNCRNELKRSNQLLSEC--GLNENLAPKRAKLQIVSTPWLEPKHHFEVMSEIEELDLKLHDQLFVLGLPDGEVVSGLN----------------FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRP--QRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            +L++V   A+ ++N   N    Y   +V   R++VV+G+ Y+  + L  T C+   K  + LL  C   ++  L   +    I+  PW         M+E + L      ++F++ L D E     N                F  F   +NK Y ++EE A+R +IF  N KKA ++  +D+G+A YGVT++SDLT++EFRS YL+P   LS +P  Q    I  ++      DWR  G VT VKNQG CGSCWAFS  GN+EGQWFLK   LVSLSEQ+LVDCD +D+ C GGLPS AY+AI+++GG+E+E  Y Y  +K  C+   SK++ YIN S             C++  P S+        FYR GISHPF I CNP  +DH VL+VG+    +G PFW +KNSWG  WGE GYY +YRG+G CG+N M +++V++
Sbjct:   35 ELKQVAEVAVREFNRGNNGEEIYRQIRVTQARKKVVSGLNYDLTLILGRTQCK---KGQDFLLGSCLQTMSPPLMGVKCTFSILIVPW---------MNETKILHQMCSQEIFIVELTDTETSQKQNVPSSELEDEMLKTLTLFKDFVTTYNKKYSDQEEAARRLQIFSQNLKKAQMIQEMDQGTAEYGVTKYSDLTEDEFRSLYLNPL--LSSKPLYQMKKAIVPNMSAPDQWDWRDHGAVTEVKNQGMCGSCWAFSVIGNIEGQWFLKKGSLVSLSEQELVDCDGVDHACAGGLPSNAYEAIEKLGGIETEQEYSYEGHKNTCSFSTSKVSAYINSS-------------CTLITPFSLDFTL----FYRKGISHPFRILCNPWMIDHAVLLVGYGE-RNGTPFWAIKNSWGTDWGEQGYYYLYRGTGACGMNTMCSSAVVD 446          
BLAST of Cathepsin F vs. Ensembl Mouse
Match: Ctsf (cathepsin F [Source:MGI Symbol;Acc:MGI:1861434])

HSP 1 Score: 299.671 bits (766), Expect = 4.590e-95
Identity = 150/300 (50.00%), Postives = 196/300 (65.33%), Query Frame = 2
Query:  683 FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDL-SKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            F  F   +N+ Y++ EE   R  +F  N  +A  +  LD+G+A YG+T+FSDLT+EEF + YL+P     S R    ++   D+   +  DWR  G VT VKNQG CGSCWAFS TGNVEGQWFL    L+SLSEQ+L+DCD +D  C GGLPS AY AI+ +GGLE+ED Y Y  +   C        VYIN S  +  +E+ +A W +  GPIS+ INAF MQFYR GI+HPF   C+P  +DH VL+VG+ +  S  P+W +KNSWG  WGE GYY +YRGSG CG+N MA+++V+N
Sbjct:  165 FKDFMTTYNRTYESREEAQWRLTVFARNMIRAQKIQALDRGTAQYGITKFSDLTEEEFHTIYLNPLLQKESGRKMSPAKSINDLAPPE-WDWRKKGAVTEVKNQGMCGSCWAFSVTGNVEGQWFLNRGTLLSLSEQELLDCDKVDKACLGGLPSNAYAAIKNLGGLETEDDYGYQGHVQTCNFSAQMAKVYINDSVELSRNENKIAAWLAQKGPISVAINAFGMQFYRHGIAHPFRPLCSPWFIDHAVLLVGYGN-RSNIPYWAIKNSWGSDWGEEGYYYLYRGSGACGVNTMASSAVVN 462          
BLAST of Cathepsin F vs. Ensembl Mouse
Match: Ctsw (cathepsin W [Source:MGI Symbol;Acc:MGI:1338045])

HSP 1 Score: 210.69 bits (535), Expect = 3.226e-62
Identity = 110/325 (33.85%), Postives = 170/325 (52.31%), Query Frame = 2
Query:  683 FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKT-------AQAVDWR-ALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKG--NCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSG----------------EPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            F  F+  FN+ Y N  E  +R  IF  N  +A  + + D G+A +G T FSDLT+EEF        Y   + P+R   + + +++        +  DWR A   +++VKNQGSC  CWA +   N++  W +K ++ V +S Q+L+DC+   NGCNGG    AY  +    GL SE  YP+  ++    C   K K   +I     + ++E  +A++ +V+GPI++ IN   +Q Y+ G+       C+P  +DH VL+VGF     G                 P+WI+KNSWG  WGE GY+ +YRG+  CG+ K   T+ ++
Sbjct:   40 FKLFQIRFNRSYWNPAEYTRRLSIFAHNLAQAQRLQQEDLGTAEFGETPFSDLTEEEF-----GQLYGQERSPERTPNMTKKVESNTWGESVPRTCDWRKAKNIISSVKNQGSCKCCWAMAAADNIQALWRIKHQQFVDVSVQELLDCERCGNGCNGGFVWDAYLTVLNNSGLASEKDYPFQGDRKPHRCLAKKYKKVAWIQDFTMLSNNEQAIAHYLAVHGPITVTINMKLLQHYQKGVIKATPSSCDPRQVDHSVLLVGFGKEKEGMQTGTVLSHSRKRRHSSPYWILKNSWGAHWGEKGYFRLYRGNNTCGVTKYPFTAQVD 359          
BLAST of Cathepsin F vs. Ensembl Mouse
Match: Ctsl (cathepsin L [Source:MGI Symbol;Acc:MGI:88564])

HSP 1 Score: 201.83 bits (512), Expect = 3.033e-59
Identity = 123/328 (37.50%), Postives = 176/328 (53.66%), Query Frame = 2
Query:  641 VLGLPDGEVVSGLNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYG-------VTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTI--DNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAF--AMQFYRGGISHPFSIFCNPNHLDHGVLIVGF---NSTTSGEPFWIVKNSWGESWGENGYYLVYRG-SGVCGLNKMATTSVIN 1579
             L  P  +      +  +K    +LY   EEE +R     + ++K + M +L  G    G       +  F D+T+EEFR Q ++       +  R+ Q P  +K  ++VDWR  G VT VKNQG CGSCWAFS +G +EGQ FLKT +L+SLSEQ LVDC     + GCNGGL   A++ I++ GGL+SE+SYPY A  G+C            G   I   E  +    +  GPIS+ ++A   ++QFY  GI   +   C+  +LDHGVL+VG+    + ++   +W+VKNSWG  WG  GY  + +     CGL   A+  V+N
Sbjct:   15 ALATPKFDQTFSAEWHQWKSTHRRLYGTNEEEWRR-----AIWEKNMRMIQLHNGEYSNGQHGFSMEMNAFGDMTNEEFR-QVVNGYRHQKHKKGRLFQEPLMLKIPKSVDWREKGCVTPVKNQGQCGSCWAFSASGCLEGQMFLKTGKLISLSEQNLVDCSHAQGNQGCNGGLMDFAFQYIKENGGLDSEESYPYEAKDGSCKYRAEFAVANDTGFVDIPQQEKALMKAVATVGPISVAMDASHPSLQFYSSGIY--YEPNCSSKNLDHGVLLVGYGYEGTDSNKNKYWLVKNSWGSEWGMEGYIKIAKDRDNHCGLATAASYPVVN 334          
BLAST of Cathepsin F vs. Ensembl Mouse
Match: Ctsl (cathepsin L [Source:MGI Symbol;Acc:MGI:88564])

HSP 1 Score: 201.83 bits (512), Expect = 3.033e-59
Identity = 123/328 (37.50%), Postives = 176/328 (53.66%), Query Frame = 2
Query:  641 VLGLPDGEVVSGLNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYG-------VTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTI--DNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAF--AMQFYRGGISHPFSIFCNPNHLDHGVLIVGF---NSTTSGEPFWIVKNSWGESWGENGYYLVYRG-SGVCGLNKMATTSVIN 1579
             L  P  +      +  +K    +LY   EEE +R     + ++K + M +L  G    G       +  F D+T+EEFR Q ++       +  R+ Q P  +K  ++VDWR  G VT VKNQG CGSCWAFS +G +EGQ FLKT +L+SLSEQ LVDC     + GCNGGL   A++ I++ GGL+SE+SYPY A  G+C            G   I   E  +    +  GPIS+ ++A   ++QFY  GI   +   C+  +LDHGVL+VG+    + ++   +W+VKNSWG  WG  GY  + +     CGL   A+  V+N
Sbjct:   15 ALATPKFDQTFSAEWHQWKSTHRRLYGTNEEEWRR-----AIWEKNMRMIQLHNGEYSNGQHGFSMEMNAFGDMTNEEFR-QVVNGYRHQKHKKGRLFQEPLMLKIPKSVDWREKGCVTPVKNQGQCGSCWAFSASGCLEGQMFLKTGKLISLSEQNLVDCSHAQGNQGCNGGLMDFAFQYIKENGGLDSEESYPYEAKDGSCKYRAEFAVANDTGFVDIPQQEKALMKAVATVGPISVAMDASHPSLQFYSSGIY--YEPNCSSKNLDHGVLLVGYGYEGTDSNKNKYWLVKNSWGSEWGMEGYIKIAKDRDNHCGLATAASYPVVN 334          
BLAST of Cathepsin F vs. Ensembl Mouse
Match: Ctsll3 (cathepsin L-like 3 [Source:MGI Symbol;Acc:MGI:1917452])

HSP 1 Score: 192.586 bits (488), Expect = 7.949e-56
Identity = 114/307 (37.13%), Postives = 165/307 (53.75%), Query Frame = 2
Query:  683 FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYG----VTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDC--DTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINA--FAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRG-SGVCGLNKMATTSVI 1576
            ++ +K    K Y   +E  KR      N KK + ++  D     +G    +  F DLT+ EFR          +K   ++ Q P      ++VDWR  G VT VK+QGSCGSCWAFS  G++EGQ F KT +LV LS Q LVDC     + GC+GGLP  A++ ++  GGL++  SYPY A  G C  +    A  + G  ++ SSE  +    +  GPIS+GI+    + QFY+ G+   +   C+   LDH VL+VG+   + G  +W+VKNSWG  WG NGY  + +  +  CG+   A+  V+
Sbjct:   29 WEEWKTKHKKTYNMNDEGQKR--AVWENNKKMIDLHNEDYLKGKHGFSLEMNAFGDLTNTEFRELMTGFQGQKTKMMMKVFQEPLLGDVPKSVDWRDHGYVTPVKDQGSCGSCWAFSAVGSLEGQMFRKTGKLVPLSVQNLVDCSWSQGNQGCDGGLPDLAFQYVKDNGGLDTSVSYPYEALNGTCRYNPKNSAATVTGFVNVQSSEDALMKAVATVGPISVGIDTKHKSFQFYKEGMY--YEPDCSSTVLDHAVLVVGYGEESDGRKYWLVKNSWGRDWGMNGYIKMAKDRNNNCGIASDASYPVV 331          
BLAST of Cathepsin F vs. UniProt/SwissProt
Match: sp|Q26534|CATL_SCHMA (Cathepsin L OS=Schistosoma mansoni OX=6183 GN=CL1 PE=2 SV=1)

HSP 1 Score: 322.013 bits (824), Expect = 8.897e-105
Identity = 157/297 (52.86%), Postives = 204/297 (68.69%), Query Frame = 2
Query:  692 FKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDL-SKRPQRISQIPQDIKT-AQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVI 1576
            FK  + K Y   E+E  RF IF SN  KA L     +GSA+YGVT +SDLT +EF   +L+ ++ + S R    + + +++    +  DWR  G VT VKNQG CGSCWAFSTTGNVE QWF KT +L+SLSEQQLVDCD +D+GCNGGLPS AY++I +MGGL  ED+YPY+A    C L    +AVYIN S ++   E+ +A W   N  IS+G+NA  +QFY+ GISHP+ IFC+   LDH VL+VG+  +   EPFWIVKNSWG  WGENGY+ +YRG G CG+N +AT+++I
Sbjct:   23 FKLKYRKQYHETEDEI-RFNIFKSNILKAQLYQVFVRGSAIYGVTPYSDLTTDEFARTHLTASWVVPSSRSNTPTSLGKEVNNIPKNFDWREKGAVTEVKNQGMCGSCWAFSTTGNVESQWFRKTGKLLSLSEQQLVDCDGLDDGCNGGLPSNAYESIIKMGGLMLEDNYPYDAKNEKCHLKTDGVAVYINSSVNLTQDETELAAWLYHNSTISVGMNALLLQFYQHGISHPWWIFCSKYLLDHAVLLVGYGVSEKNEPFWIVKNSWGVEWGENGYFRMYRGDGSCGINTVATSAMI 318          
BLAST of Cathepsin F vs. UniProt/SwissProt
Match: sp|Q9R013|CATF_MOUSE (Cathepsin F OS=Mus musculus OX=10090 GN=Ctsf PE=1 SV=1)

HSP 1 Score: 299.671 bits (766), Expect = 3.211e-94
Identity = 150/300 (50.00%), Postives = 196/300 (65.33%), Query Frame = 2
Query:  683 FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDL-SKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            F  F   +N+ Y++ EE   R  +F  N  +A  +  LD+G+A YG+T+FSDLT+EEF + YL+P     S R    ++   D+   +  DWR  G VT VKNQG CGSCWAFS TGNVEGQWFL    L+SLSEQ+L+DCD +D  C GGLPS AY AI+ +GGLE+ED Y Y  +   C        VYIN S  +  +E+ +A W +  GPIS+ INAF MQFYR GI+HPF   C+P  +DH VL+VG+ +  S  P+W +KNSWG  WGE GYY +YRGSG CG+N MA+++V+N
Sbjct:  165 FKDFMTTYNRTYESREEAQWRLTVFARNMIRAQKIQALDRGTAQYGITKFSDLTEEEFHTIYLNPLLQKESGRKMSPAKSINDLAPPE-WDWRKKGAVTEVKNQGMCGSCWAFSVTGNVEGQWFLNRGTLLSLSEQELLDCDKVDKACLGGLPSNAYAAIKNLGGLETEDDYGYQGHVQTCNFSAQMAKVYINDSVELSRNENKIAAWLAQKGPISVAINAFGMQFYRHGIAHPFRPLCSPWFIDHAVLLVGYGN-RSNIPYWAIKNSWGSDWGEEGYYYLYRGSGACGVNTMASSAVVN 462          
BLAST of Cathepsin F vs. UniProt/SwissProt
Match: sp|Q9UBX1|CATF_HUMAN (Cathepsin F OS=Homo sapiens OX=9606 GN=CTSF PE=1 SV=1)

HSP 1 Score: 295.434 bits (755), Expect = 3.084e-92
Identity = 152/307 (49.51%), Postives = 201/307 (65.47%), Query Frame = 2
Query:  683 FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQAV--------DWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            F +F   +N+ Y+++EE   R  +F +N  +A  +  LD+G+A YGVT+FSDLT+EEFR+ YL+    L K P         +K A++V        DWR+ G VT VK+QG CGSCWAFS TGNVEGQWFL    L+SLSEQ+L+DCD +D  C GGLPS AY AI+ +GGLE+ED Y Y  +  +C     K  VYIN S  +  +E  +A W +  GPIS+ INAF MQFYR GIS P    C+P  +DH VL+VG+ +  S  PFW +KNSWG  WGE GYY ++RGSG CG+N MA+++V++
Sbjct:  187 FKNFVITYNRTYESKEEARWRLSVFVNNMVRAQKIQALDRGTAQYGVTKFSDLTEEEFRTIYLNTL--LRKEPG------NKMKQAKSVGDLAPPEWDWRSKGAVTKVKDQGMCGSCWAFSVTGNVEGQWFLNQGTLLSLSEQELLDCDKMDKACMGGLPSNAYSAIKNLGGLETEDDYSYQGHMQSCNFSAEKAKVYINDSVELSQNEQKLAAWLAKRGPISVAINAFGMQFYRHGISRPLRPLCSPWLIDHAVLLVGYGN-RSDVPFWAIKNSWGTDWGEKGYYYLHRGSGACGVNTMASSAVVD 484          
BLAST of Cathepsin F vs. UniProt/SwissProt
Match: sp|Q9VN93|CPR1_DROME (Putative cysteine proteinase CG12163 OS=Drosophila melanogaster OX=7227 GN=CG12163 PE=2 SV=2)

HSP 1 Score: 285.034 bits (728), Expect = 6.847e-87
Identity = 142/312 (45.51%), Postives = 189/312 (60.58%), Query Frame = 2
Query:  683 FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKR--------PQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASI-DSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGE-----PFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVI 1576
            F  F+  F + Y +  E   R +IF  N K    +N  + GSA YG+T+F+D+T  E++ +      D +K         P    ++P++       DWR    VT VKNQGSCGSCWAFS TGN+EG + +KT  L   SEQ+L+DCDT D+ CNGGL   AYKAI+ +GGLE E  YPY A K  C  +++   V + G   +   +E+ M  W   NGPISIGINA AMQFYRGG+SHP+   C+  +LDHGVL+VG+  +         P+WIVKNSWG  WGE GYY VYRG   CG+++MAT++V+
Sbjct:  308 FYKFQVRFGRRYVSTAERQMRLRIFRQNLKTIEELNANEMGSAKYGITEFADMTSSEYKERTGLWQRDEAKATGGSAAVVPAYHGELPKEF------DWRQKDAVTQVKNQGSCGSCWAFSVTGNIEGLYAVKTGELKEFSEQELLDCDTTDSACNGGLMDNAYKAIKDIGGLEYEAEYPYKAKKNQCHFNRTLSHVQVAGFVDLPKGNETAMQEWLLANGPISIGINANAMQFYRGGVSHPWKALCSKKNLDHGVLVVGYGVSDYPNFHKTLPYWIVKNSWGPRWGEQGYYRVYRGDNTCGVSEMATSAVL 613          
BLAST of Cathepsin F vs. UniProt/SwissProt
Match: sp|Q8VYS0|RD19D_ARATH (Probable cysteine protease RD19D OS=Arabidopsis thaliana OX=3702 GN=RD19D PE=2 SV=1)

HSP 1 Score: 263.077 bits (671), Expect = 2.743e-81
Identity = 142/319 (44.51%), Postives = 187/319 (58.62%), Query Frame = 2
Query:  683 FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKR--------PQ-RISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCD---------TIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNST------TSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATT 1567
            F  F  ++ K Y   EE   R  IF  N  KA     +D  SAV+GVTQFSDLT+EEF+  Y   A     R        P   +  +P+D       DWR  GGVT VKNQG+CGSCWAFSTTG  EG  F+ T +L+SLSEQQLVDCD           DNGC GGL + AY+ + + GGLE E SYPY   +G+C  D  K+AV +    +I   E+ +A     +GP+++G+NA  MQ Y GG+S P  + C+  +++HGVL+VG+ S        S +P+WI+KNSWG+ WGENGYY + RG  +CG+N M + 
Sbjct:   51 FRLFMSDYGKNYSTREEYIHRLGIFAKNVLKAAEHQMMDP-SAVHGVTQFSDLTEEEFKRMYTGVADVGGSRGGTVGAEAPMVEVDGLPEDF------DWREKGGVTEVKNQGACGSCWAFSTTGAAEGAHFVSTGKLLSLSEQQLVDCDQACDPKDKKACDNGCGGGLMTNAYEYLMEAGGLEEERSYPYTGKRGHCKFDPEKVAVRVLNFTTIPLDENQIAANLVRHGPLAVGLNAVFMQTYIGGVSCP--LICSKRNVNHGVLLVGYGSKGFSILRLSNKPYWIIKNSWGKKWGENGYYKLCRGHDICGINSMVSA 360          
BLAST of Cathepsin F vs. TrEMBL
Match: A0A267DEW1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003424g1 PE=3 SV=1)

HSP 1 Score: 431.409 bits (1108), Expect = 7.241e-143
Identity = 215/449 (47.88%), Postives = 273/449 (60.80%), Query Frame = 2
Query:  260 TGEKREISLSKLRKDKDLQEVFREAINKYNSRVNSIYSYAPYQVHSIRQQVVAGILYEFEVTLVGTNCRNELKRSNQLLSECGLNENLAPK----RAKLQIVSTPWLEPKHH------FEVMSEIEELDLKLHDQLFVLGLPDGEVVSGLNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVI 1576
            TG K  I+ ++  + +  Q    E    YN+  NS++ +A  +    ++QVVAGI Y F    V T CR  ++     L  C   E L+P     R  + +V  PW  P+ +       E   +   L         +LG    ++     F  FK+   K+Y +  EE +RF IF  N KKA     LD+G A YG + F+DLT EEFRS+YLSP +DLS+RP   + +P       + DWR  G VT VKNQG+CGSCWAFSTTGN+EGQWF+  K+LV LSEQQLVDCD +D GCNGGLPSQAYK I++MGGL  E  YPY  +   C  ++ K+AVYIN S +I   E  MA W   NGPISIGINA AMQFY  GISHP+ IFCNP+HLDHGVLIVG+    + EP+WI+KNSWG SWG  GYYLVYRG G CGLN+M T++ +
Sbjct:   26 TGGKSPIT-AQDAEGESFQNAITEVTRAYNTYSNSMFHHAMLRYEDAKKQVVAGIRYSFVAVFVETPCRKAVRHYGSPLRWC--KEKLSPDAKVYRCPVSVVYRPWSSPRQYEVDRSGLESCQQTRRLKRSEPQHQVLLGGDSHDIGHYGQFAEFKRAHGKVYASRAEEQRRFAIFRDNMKKAYAYQMLDRGDARYGASVFADLTQEEFRSRYLSPRWDLSRRPAEEAAVPTLGDLPTSFDWREKGAVTEVKNQGTCGSCWAFSTTGNIEGQWFISKKKLVDLSEQQLVDCDKVDEGCNGGLPSQAYKEIERMGGLMKESDYPYRGSDQKCQFNQGKVAVYINSSVAISKDEKAMAAWLVQNGPISIGINANAMQFYLSGISHPWKIFCNPSHLDHGVLIVGYGMKGT-EPYWIIKNSWGTSWGNKGYYLVYRGDGTCGLNQMCTSAQV 470          
BLAST of Cathepsin F vs. TrEMBL
Match: A0A267FPA5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003424g2 PE=3 SV=1)

HSP 1 Score: 431.024 bits (1107), Expect = 9.496e-143
Identity = 215/449 (47.88%), Postives = 273/449 (60.80%), Query Frame = 2
Query:  260 TGEKREISLSKLRKDKDLQEVFREAINKYNSRVNSIYSYAPYQVHSIRQQVVAGILYEFEVTLVGTNCRNELKRSNQLLSECGLNENLAPK----RAKLQIVSTPWLEPKHH------FEVMSEIEELDLKLHDQLFVLGLPDGEVVSGLNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVI 1576
            TG K  I+ ++  + +  Q    E    YN+  NS++ +A  +    ++QVVAGI Y F    V T CR  ++     L  C   E L+P     R  + +V  PW  P+ +       E   +   L         +LG    ++     F  FK+   K+Y +  EE +RF IF  N KKA     LD+G A YG + F+DLT EEFRS+YLSP +DLS+RP   + +P       + DWR  G VT VKNQG+CGSCWAFSTTGN+EGQWF+  K+LV LSEQQLVDCD +D GCNGGLPSQAYK I++MGGL  E  YPY  +   C  ++ K+AVYIN S +I   E  MA W   NGPISIGINA AMQFY  GISHP+ IFCNP+HLDHGVLIVG+    + EP+WI+KNSWG SWG  GYYLVYRG G CGLN+M T++ +
Sbjct:   26 TGGKSPIT-AQDAEGESFQNAITEVTRAYNTYSNSMFHHAMLRYEDAKKQVVAGIRYTFVAVFVETPCRKAVRHYGSPLRWC--KEKLSPDAKVYRCPVSVVYRPWSSPRQYEVDRSGLESCQQTRRLKRSEPQHQVLLGGDSHDIGHYGQFAEFKRAHGKVYASRAEEQRRFAIFRDNMKKAYAYQMLDRGDARYGASVFADLTQEEFRSRYLSPRWDLSRRPAEEAAVPTLGDLPTSFDWREKGAVTEVKNQGTCGSCWAFSTTGNIEGQWFISKKKLVDLSEQQLVDCDKVDEGCNGGLPSQAYKEIERMGGLMKESDYPYRGSDQKCQFNQGKVAVYINSSVAISKDEKAMAAWLVQNGPISIGINANAMQFYLSGISHPWKIFCNPSHLDHGVLIVGYGMKGT-EPYWIIKNSWGTSWGNKGYYLVYRGDGTCGLNQMCTSAQV 470          
BLAST of Cathepsin F vs. TrEMBL
Match: A0A267FSZ7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig021148g1 PE=3 SV=1)

HSP 1 Score: 429.098 bits (1102), Expect = 6.475e-142
Identity = 214/433 (49.42%), Postives = 268/433 (61.89%), Query Frame = 2
Query:  311 LQEVFREAINKYNSRVNSIYSYAPYQVHSIRQQVVAGILYEFEVTLVGTNCRNELKRSNQLLSECGLNENLAPK----RAKLQIVSTPWLEPKHHFEVMSEIEELD----LKLHDQL---FVLGLPDGEVVSGLNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVI 1576
             Q    E    YN+  NS++ +A  +    ++QVVAGI Y F    V T CR  ++     L  C   E L+P     R  + +V  PW  P+ +    S +E       LK  + L    +LG    ++     F  FK+   K+Y +  EE +RF IF  N KKA     LD+G A YG + F+DLT EEFRS+YLSP +DLS+RP   + +P       + DWR  G VT VKNQG+CGSCWAFSTTGN+EGQWF+  K+LV LSEQQLVDCD +D GCNGGLPSQAYK I++MGGL  E  YPY  +   C  ++ K+AVYIN S +I   E  MA W   NGPISIGINA AMQFY  GISHP+ IFCNP+HLDHGVLIVG+    + EP+WI+KNSWG SWG  GYYLVYRG G CGLN+M T++ +
Sbjct:   42 FQNAITEVTRAYNTYSNSMFHHAMLRYEDAKKQVVAGIRYTFVAVFVETPCRKAVRHYGSPLRWC--KEKLSPDAKVYRCPVSVVYRPWSSPRQYEVDRSGLESCQQTRRLKRSEPLQHQVLLGGDSHDIGHYGQFAEFKRAHGKVYASRAEEQRRFAIFRDNMKKAYAYQMLDRGDARYGASVFADLTQEEFRSRYLSPRWDLSRRPAEEAAVPTLGDLPTSFDWREKGAVTEVKNQGTCGSCWAFSTTGNIEGQWFINKKKLVDLSEQQLVDCDKVDEGCNGGLPSQAYKEIERMGGLMKESDYPYRGSDQKCQFNQGKVAVYINSSVAISKDEKAMAAWLVQNGPISIGINANAMQFYLSGISHPWKIFCNPSHLDHGVLIVGYGMKGT-EPYWIIKNSWGTSWGNKGYYLVYRGDGTCGLNQMCTSAQV 471          
BLAST of Cathepsin F vs. TrEMBL
Match: A0A1I8IVL0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 434.491 bits (1116), Expect = 6.414e-136
Identity = 211/433 (48.73%), Postives = 264/433 (60.97%), Query Frame = 2
Query:  311 LQEVFREAINKYNSRVNSIYSYAPYQVHSIRQQVVAGILYEFEVTLVGTNCRNELKRSNQLLSECGLNENLAPK----RAKLQIVSTPWLEPKHH------FEVMSEIEELDLKLHDQLFVLGLPDGEVVSGLNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
             Q    E    YN+  NS++ +A  +    ++QVVAGI Y F    V T CR  ++     L  C   E L+P     R  + +V  PW  P+ +       E   +   L         +LG    ++     F  FK+   K+Y +  EE +RF IF  N KKA     LD+G A YG + F+DLT EEFRS+YLSP +DLS+RP   + +P       + DWR  G VT VKNQG+CGSCWAFSTTGN+EGQWF+  K+LV LSEQQLVDCD +D GCNGGLPSQAYK I++MGGL  E  YPY  +   C  ++ K+AVYIN S +I   E  MA W   NGPISIGINA AMQFY  GISHP+ IFCNP+HLDHGVLIVG+    + EP+WI+KNSWG SWG  GYYLVYRG G CGLN+M T++ + 
Sbjct:  737 FQNAITEVTRAYNTYSNSMFHHAMLRYEDAKKQVVAGIRYSFVAVFVETPCRKAVRHYGSPLRWC--KEKLSPDAKVYRCPVSVVYRPWSSPRQYEVDRSGLESCQQTRRLKRSEPQHQVLLGGDSHDIGHYGQFAEFKRAHGKVYASRAEEQRRFAIFRDNMKKAYAYQMLDRGDARYGASVFADLTQEEFRSRYLSPRWDLSRRPAEEAAVPTLGDLPTSFDWREKGAVTEVKNQGTCGSCWAFSTTGNIEGQWFISKKKLVDLSEQQLVDCDKVDEGCNGGLPSQAYKEIERMGGLMKESDYPYRGSDQKCQFNQGKVAVYINSSVAISKDEKAMAAWLVQNGPISIGINANAMQFYLSGISHPWKIFCNPSHLDHGVLIVGYGMKGT-EPYWIIKNSWGTSWGNKGYYLVYRGDGTCGLNQMCTSAQVK 1166          
BLAST of Cathepsin F vs. TrEMBL
Match: A0A1I8I5R8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 429.869 bits (1104), Expect = 2.238e-134
Identity = 214/431 (49.65%), Postives = 267/431 (61.95%), Query Frame = 2
Query:  311 LQEVFREAINKYNSRVNSIYSYAPYQVHSIRQQVVAGILYEFEVTLVGTNCRNELKRSNQLLSECGLNENLAPK----RAKLQIVSTPWLEPKHHFEVMSEIEELD----LKLHDQL---FVLGLPDGEVVSGLNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTS 1570
             Q    E    YN+  NS++ +A  +    ++QVVAGI Y F    V T CR  ++     L  C   E L+P     R  + +V  PW  P+ +    S +E       LK  + L    +LG    ++     F  FK+   K+Y +  EE +RF IF  N KKA     LD+G A YG + F+DLT EEFRS+YLSP +DLS+RP   + +P       + DWR  G VT VKNQG+CGSCWAFSTTGN+EGQWF+  K+LV LSEQQLVDCD +D GCNGGLPSQAYK I++MGGL  E  YPY  +   C  ++ K+AVYIN S +I   E  MA W   NGPISIGINA AMQFY  GISHP+ IFCNP+HLDHGVLIVG+    + EP+WI+KNSWG SWG  GYYLVYRG G CGLN+M T++
Sbjct:  538 FQNAITEVTRAYNTYSNSMFHHAMLRYEDAKKQVVAGIRYTFVAVFVETPCRKAVRHYGSPLRWC--KEKLSPDAKVYRCPVSVVYRPWSSPRQYEVDRSGLESCQQTRRLKRSEPLQHQVLLGGDSHDIGHYGQFAEFKRAHGKVYASRAEEQRRFAIFRDNMKKAYAYQMLDRGDARYGASVFADLTQEEFRSRYLSPRWDLSRRPAEEAAVPTLGDLPTSFDWREKGAVTEVKNQGTCGSCWAFSTTGNIEGQWFINKKKLVDLSEQQLVDCDKVDEGCNGGLPSQAYKEIERMGGLMKESDYPYRGSDQKCQFNQGKVAVYINSSVAISKDEKAMAAWLVQNGPISIGINANAMQFYLSGISHPWKIFCNPSHLDHGVLIVGYGMKGT-EPYWIIKNSWGTSWGNKGYYLVYRGDGTCGLNQMCTSA 965          
BLAST of Cathepsin F vs. Ensembl Cavefish
Match: ctsf (cathepsin F [Source:NCBI gene;Acc:103042155])

HSP 1 Score: 345.51 bits (885), Expect = 9.037e-113
Identity = 188/439 (42.82%), Postives = 264/439 (60.14%), Query Frame = 2
Query:  302 DKDLQEVFREAINKYNSRVNSIYSYAPYQVHSIRQQVVAGILYEFEVTLVGTNCRNELKRSNQL-LSE-CGLNENLAPKRAKL---QIVSTPW------LEPKHHFEVMSEIEELDLKL-HDQLFVLGLPDGEVVSGLNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAY-DLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            D +L +  R A  +YN   N ++     ++ S  +Q+V GI Y   V +  T C    K+++ L +SE C        ++ ++   ++   PW      L+ K    V  E + + + L  D+     L   E+++   F  F   +N+ Y ++EE  KR +IF  N K A  +  LD+G+A YGVT+FSDLT++EFR  YL+P     S + +     P     A++ DWR  G V+ VKNQG CGSCWAFS TGN+EGQWF KT +L+SLSEQ+LVDCD +D  C GGLPS AY+AI+ +GGLE+E  Y Y  +K +C     K+A YIN S  +   E+ +A W   NGP+S  +NAFAMQFYR G+SHP  IFCNP  +DH VL+VGF    +G PFW +KNSWGE +GE GYY +Y+GS +CG+NKM +++V+N
Sbjct:   42 DPELLKAVRFAEERYNVASNGLHIRRVSRIISASKQLVKGIRYSITVEIGRTQC----KKTDVLHVSEDCEFFPESHKQKTEVCLFEVWDIPWQNKSTLLKQKCQPAVPKETKIVRVPLTRDKTTKESL---ELLT--KFKDFMTTYNRSYISQEEAQKRLRIFQQNLKTAETLQSLDQGTAEYGVTKFSDLTEDEFRMMYLNPMLSQWSLQKKLKPAAPASKAAAESWDWREHGAVSPVKNQGMCGSCWAFSVTGNIEGQWFKKTGQLLSLSEQELVDCDKLDQACGGGLPSNAYEAIENLGGLETETDYTYTGHKQSCGFTSGKVAAYINSSVELSKDENDIAAWLVENGPVSAALNAFAMQFYRKGVSHPLKIFCNPWMIDHAVLLVGFGE-RNGIPFWAIKNSWGEDYGEQGYYYLYKGSRLCGINKMCSSAVVN 470          
BLAST of Cathepsin F vs. Ensembl Cavefish
Match: ctsf (cathepsin F [Source:NCBI gene;Acc:103042155])

HSP 1 Score: 343.199 bits (879), Expect = 4.317e-112
Identity = 183/433 (42.26%), Postives = 256/433 (59.12%), Query Frame = 2
Query:  302 DKDLQEVFREAINKYNSRVNSIYSYAPYQVHSIRQQVVAGILYEFEVTLVGTNCRNELKRSNQL-LSE-CGLNENLAPKRAKL---QIVSTPWLEPKHHFEVMSEIEEL-DLKLHDQLFVLGLPDGEVVSGLNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAY-DLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            D +L +  R A  +YN   N ++     ++ S  +Q+V GI Y   V +  T C    K+++ L +SE C        ++ ++   ++   PW       +   +   L   +  + L +L            F  F   +N+ Y ++EE  KR +IF  N K A  +  LD+G+A YGVT+FSDLT++EFR  YL+P     S + +     P     A++ DWR  G V+ VKNQG CGSCWAFS TGN+EGQWF KT +L+SLSEQ+LVDCD +D  C GGLPS AY+AI+ +GGLE+E  Y Y  +K +C     K+A YIN S  +   E+ +A W   NGP+S  +NAFAMQFYR G+SHP  IFCNP  +DH VL+VGF    +G PFW +KNSWGE +GE GYY +Y+GS +CG+NKM +++V+N
Sbjct:   42 DPELLKAVRFAEERYNVASNGLHIRRVSRIISASKQLVKGIRYSITVEIGRTQC----KKTDVLHVSEDCEFFPESHKQKTEVCLFEVWDIPWQNKSTLLKQKCQPAGLCSFRTTESLELL----------TKFKDFMTTYNRSYISQEEAQKRLRIFQQNLKTAETLQSLDQGTAEYGVTKFSDLTEDEFRMMYLNPMLSQWSLQKKLKPAAPASKAAAESWDWREHGAVSPVKNQGMCGSCWAFSVTGNIEGQWFKKTGQLLSLSEQELVDCDKLDQACGGGLPSNAYEAIENLGGLETETDYTYTGHKQSCGFTSGKVAAYINSSVELSKDENDIAAWLVENGPVSAALNAFAMQFYRKGVSHPLKIFCNPWMIDHAVLLVGFGE-RNGIPFWAIKNSWGEDYGEQGYYYLYKGSRLCGINKMCSSAVVN 459          
BLAST of Cathepsin F vs. Ensembl Cavefish
Match: ENSAMXT00000001970.2 (cathepsin L1-like [Source:NCBI gene;Acc:103022163])

HSP 1 Score: 207.608 bits (527), Expect = 1.273e-61
Identity = 123/313 (39.30%), Postives = 182/313 (58.15%), Query Frame = 2
Query:  677 LNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRL-DKGSAVY--GVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQI----PQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCD-TIDN-GCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASI-DSSESFMANWCSVNGPISIGINA--FAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGS-GVCGLNKMATTSVI 1576
            L F+S+K    K Y +EEEE++R  I+ +N K  L  N L D+G   Y  G+  F+D+ ++EF++ +        +     +       +D    Q VDWRA G VT VKNQG CGSCWAFS TG +EGQ F KT +L+SLSEQQLVDC  +  N GCNGG PS+++  I+   GLE++ +YPY A K  C  +  ++    +G  S+    E+ + N  +  GPIS+ I+      Q Y+ G+       C+   L+H VL+VG+++  +G+P+W+VKNSWG +WG+NGY  + R     CG+  +AT  ++
Sbjct:   24 LEFNSWKLQHGKSYGSEEEESQRKMIWLTNRKLVLKHNMLADQGLKSYRLGMNIFADMKNQEFQAMFSHCVKSFDETDSLCTSTFLSEDEDAPLPQRVDWRAKGYVTFVKNQGICGSCWAFSATGALEGQMFRKTGKLISLSEQQLVDCSQSFGNLGCNGGWPSKSFDYIKSSKGLEADFTYPYKARKEICRFNPQRVIATCSGFKSLPKGDENALKNAVASIGPISVLIDTSKHTFQLYKSGVYDEPG--CSSTKLNHAVLLVGYDNDPTGKPYWLVKNSWGINWGDNGYIKMSRNKMNQCGIATIATYPLV 334          
BLAST of Cathepsin F vs. Ensembl Cavefish
Match: ctsl.1 (cathepsin L1-like [Source:NCBI gene;Acc:103023607])

HSP 1 Score: 202.986 bits (515), Expect = 7.729e-60
Identity = 123/313 (39.30%), Postives = 180/313 (57.51%), Query Frame = 2
Query:  677 LNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRL-DKGSAVY--GVTQFSDLTDEEFRSQYLS--PAYDLSKRPQRISQIPQDIKTA--QAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDC--DTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDS-SESFMANWCSVNGPISIGINA--FAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRG-SGVCGLNKMATTSVI 1576
            L F ++K  F K+Y + EEE++R   + +N K  L+ N L D+G   Y  G+T F+D+ ++E+R  + S   +++ +K     + + Q ++      VDWR  G VT +K+Q  CGSCWAFS TG++EGQ F KT +LVSLSEQQLVDC  D  + GC GGL  QA++ I+   G+++EDSYPY A  G+C      +     G   I S  E  +    +  GPIS+ I+A   + Q Y  GI +     C+   LDHGVL VG+  T +G+ +W+VKNSWG  WG  GY  + R     CG+  M++  ++
Sbjct:   24 LEFHAWKLKFGKIYHSVEEESQRKLTWLTNRKLVLVHNMLADQGIKSYRLGMTFFADMDNKEYRKLFASCLGSFNRTKAHSATTFLRQTLRDPLPDTVDWRPKGYVTDIKDQKDCGSCWAFSATGSLEGQTFRKTGKLVSLSEQQLVDCSGDYGNFGCGGGLMDQAFEYIKANKGIDTEDSYPYEAVDGDCRFKPDSVGATCTGFVDITSGDEKALQEAVATIGPISVAIDAGHSSFQLYESGIYNEPD--CSSTELDHGVLAVGYG-TDNGKDYWLVKNSWGLDWGNKGYIQMSRNEDNQCGIATMSSYPLV 333          
BLAST of Cathepsin F vs. Ensembl Cavefish
Match: ENSAMXT00000001927.2 (cathepsin L1-like [Source:NCBI gene;Acc:107197646])

HSP 1 Score: 199.904 bits (507), Expect = 1.129e-58
Identity = 117/313 (37.38%), Postives = 174/313 (55.59%), Query Frame = 2
Query:  677 LNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRL-DKGSAVY--GVTQFSDLTDEEFRSQ---YLSPAYDLSKRPQ-RISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTID--NGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASI-DSSESFMANWCSVNGPISIGINAFAMQF--YRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRG-SGVCGLNKMATTSVI 1576
            L F  +K  + K Y +EEEE++R  I+ +N K  L  N L D+G   Y  G+  F+D+ D+E++     YL P+     R   R  Q  +++    ++DWR  G V+ VK QG C SCWAFS  G +EGQ F KT  LVSLS QQLVDC  +D  +GC GG    A++ I + GGLE+E  YPY A +G C  +   ++   +    +    E  +       GP+S+ I++    F  Y+ GI +    +C+  +++HGVL+VG+N+ +SG  +W+VKNSWG  WGE GY  + R     CG+   A+  ++
Sbjct:   24 LEFQEWKLKYGKSYDSEEEESQRKMIWMTNLKLVLEHNMLADQGLKTYRLGMNHFADMDDQEYQQMFRGYLRPSNRTKPRSAGRFLQQAENVALPYSIDWRDNGYVSNVKTQGHCASCWAFSAAGALEGQMFRKTGSLVSLSMQQLVDCSWLDGNDGCGGGSMELAFEYISRSGGLETESDYPYKAREGMCMFNPQDVSGKCHDFEELPKGEEKDLQKAVVAVGPVSVAIDSSNKTFLLYQSGIYN--EPYCSSYNVNHGVLVVGYNTDSSGREYWVVKNSWGVQWGEQGYIRMSRNKDNQCGIATRASFPLV 334          
BLAST of Cathepsin F vs. Ensembl Sea Lamprey
Match: ctsf (cathepsin F [Source:ZFIN;Acc:ZDB-GENE-030131-9831])

HSP 1 Score: 313.153 bits (801), Expect = 1.876e-103
Identity = 150/292 (51.37%), Postives = 195/292 (66.78%), Query Frame = 2
Query:  716 YQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQ----RISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            + ++  E  R+++F  N+ +   +   ++GSAVYG+T+FSDLT+EEFR+ +L+P      RP+    R +QIP       A DWR  G VT VKNQG CGSCWAFS TGN+EGQW +K   L+SLSEQ+LVDCD +D GC GGL   AYK IQ++GGL +E  Y Y  +   C   ++K+  YINGS SI S E  MA W   +GPISI +NA  MQFY+ GI+HP+ I C    L+H VLIVG+  T  G PFWI+KNSWG  WGE GYY V+RG GVCGLNK  +++V++
Sbjct:    1 FSSKTAEKMRYEVFRENWHRVQKLQETEQGSAVYGITKFSDLTEEEFRALHLNPVLS---RPREVGLRQAQIPPG-PIPPAFDWRDRGAVTEVKNQGECGSCWAFSVTGNIEGQWAIKKGSLLSLSEQELVDCDKLDYGCGGGLQMNAYKTIQELGGLMTESDYSYKGDMEQCGFSQAKVRAYINGSVSISSDEDEMAAWLVKHGPISIALNALGMQFYKHGIAHPWKILCWSWELNHAVLIVGYG-TEKGVPFWIIKNSWGVGWGEEGYYRVFRGDGVCGLNKDCSSAVVD 287          
BLAST of Cathepsin F vs. Ensembl Sea Lamprey
Match: ENSPMAT00000011285.1 (pep scaffold:Pmarinus_7.0:GL499136:454:1347:-1 gene:ENSPMAG00000010258.1 transcript:ENSPMAT00000011285.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 199.904 bits (507), Expect = 9.368e-60
Identity = 126/301 (41.86%), Postives = 169/301 (56.15%), Query Frame = 2
Query:  710 KLYQNEEEEAKRFKIFHSNYKKALLMN---RLDKGSAVYGVTQFSDLTDEEFRSQYLS--PAYDLSKRPQRIS-QIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTID--NGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDS-SESFMANWCSVNGPISIGINAF--AMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRG-SGVCGLNKMATTSVI 1576
            K Y   EE  +R  I+  N KK  + N    + K +   G+ +F D+T EEF+   L    + D SK  +  +   P   K  ++VDWR  G VT VK+QG CGSCWAFSTTG++EGQ   KT  LVSLSEQ LVDC + +  NGC GGL   A+  I++ GG++SE SYPY A   NC  +    A    G   I S +E  + N  +  GP+SIGI+A   + Q Y  GI   +S     N+LDHGVL+VG+        FWIVKNSWG  WG++GY L+ R    +CG+   A+  ++
Sbjct:    1 KTYDQNEEGWRRM-IWEKNLKKIAVHNLEHSMGKHTWRMGMNRFGDMTKEEFQHTMLGLRGSNDTSKASRGATFMAPAFFKAPESVDWRKQGYVTDVKDQGDCGSCWAFSTTGSLEGQHKRKTGVLVSLSEQNLVDCSSAEGNNGCKGGLMDLAFTYIKKNGGIDSEASYPYTAKDENCKYNPKNKAAEDTGFVDIPSGNEEALKNAVASVGPVSIGIDASLESFQLYSSGI---YSDSDCSNNLDHGVLVVGYGVDDDERKFWIVKNSWGTKWGDHGYILMARDKDNLCGIATAASYPLV 297          
BLAST of Cathepsin F vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010475.1 (pep scaffold:Pmarinus_7.0:GL482653:10321:33550:-1 gene:ENSPMAG00000009483.1 transcript:ENSPMAT00000010475.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 196.823 bits (499), Expect = 9.748e-59
Identity = 116/294 (39.46%), Postives = 166/294 (56.46%), Query Frame = 2
Query:  725 EEEEAKRFKIFHSNYKKALLMN---RLDKGSAVYGVTQFSDLTDEEFRSQYLS--PAYDLSKRPQRIS-QIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTID--NGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASI-DSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYR-GSGVCGLNKMATTSVI 1576
            +EEE  R  I+  N K+  + N    + K +   G+ QFSD+T EEF+   L        SK P+  +   P   +  ++VDWR  G VT +KNQG CGSCW+FSTTG++EGQ   KT  LVSLSEQ LVDC + +  +GCNGGL   A+  I++ GG++SE+SYPY      C  D    A  + G   I +  E  + N  +  GP+S+ I+A    F   G        C+ +H+DH VL+VG+     G+ FW+VKNSWG +WGE GY  + R  + +CG+  M++  ++
Sbjct:    1 QEEEGWRRIIWEKNLKRISVHNLEHSMGKHTWRMGMNQFSDMTGEEFQQVMLGFRGWNGTSKAPRGATFMAPAFFEAPESVDWRKQGYVTDIKNQGDCGSCWSFSTTGSLEGQHKRKTGVLVSLSEQNLVDCSSTEGNHGCNGGLMINAFNYIKKNGGIDSEESYPYKGQVETCKYDPKNKAAEVTGYVEIPEGDEEALMNAVASVGPVSVAIDASLASFQSYGSGIYKDAMCS-HHIDHAVLVVGYGVDEMGQKFWLVKNSWGVTWGEQGYVRMARDDNNLCGIATMSSYPLV 293          
BLAST of Cathepsin F vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006499.1 (pep scaffold:Pmarinus_7.0:GL477089:42202:47941:1 gene:ENSPMAG00000005817.1 transcript:ENSPMAT00000006499.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 191.815 bits (486), Expect = 3.538e-56
Identity = 120/316 (37.97%), Postives = 176/316 (55.70%), Query Frame = 2
Query:  683 FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMN---RLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQ-----IPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTID--NGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGN-CALDKSKIAVYINGSASIDS-SESFMANWCSVNGPISIGINAF--AMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGE---PFWIVKNSWGESWGENGYYLVYRG-SGVCGLNKMATTSVI 1576
            + S+K    KLY+ ++E  +R  I+  N  K  + N    L K +   G+  F D+T+EEFR Q ++  + +  +P ++S+     +PQ+ +    VDWR  G VT VK+QG CGSCWAFSTTG +EGQ   K  +LVSLSEQ LVDC   +   GC GGL  QA++ ++  GG++SE +YPY    G+ C  D    +    G   + S  E  +    +  GP+S+ I+A   + QFY+ G+   F   C+   LDHGVL+VG+    +GE    +WIVKNSW   WG+ GY L+ R     CG+   A+  ++
Sbjct:   30 WGSWKGFHQKLYEPKQESWRRM-IWEKNLNKIKIHNLEHSLGKHTWRMGMNHFGDMTNEEFR-QKMNGYHGV--QPHKVSKGSTFLVPQNYEAPPTVDWRDKGLVTEVKDQGECGSCWAFSTTGALEGQHMRKVGQLVSLSEQNLVDCSRAEGNQGCGGGLMDQAFQYVKDNGGIDSEAAYPYTGTDGDKCHYDPKFNSANDTGFVDVTSGDEKALKIAVASVGPVSVAIDASHESFQFYQSGVY--FEPDCSSQDLDHGVLVVGYGVKGTGENGKKYWIVKNSWSPKWGDEGYVLMARNKKNHCGIATAASYPLV 339          
BLAST of Cathepsin F vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007755.1 (pep scaffold:Pmarinus_7.0:GL476381:1843:17023:-1 gene:ENSPMAG00000007008.1 transcript:ENSPMAT00000007755.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 190.66 bits (483), Expect = 6.035e-56
Identity = 120/317 (37.85%), Postives = 174/317 (54.89%), Query Frame = 2
Query:  668 VSGLNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRL-DKGSAVY--GVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKT----AQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDT--IDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASID--SSESFMANWCSVNGPISIGINAFAMQF--YRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGY-YLVYRGSGVCGLNKMATTSVI 1576
            V  L +D++K  + K Y +E+E+A R  +F  N K+ L  N L D+G+  +  G+ ++SDL   E+  + +   ++L    +R    P  +++     + VDWR  G VT VK QG CGS WAFS TG++EGQ F  T  L SLSEQQLVDC     +NGCNGG   +A + I    G++SE SYPY    G C    + +A   +    ++  S+E  +    +  GPI+I +NA    F  Y+ G+ +  S   +PN   H +L+VG+ S  SG  FWIVKNSWGE WGE GY Y++      CG+  +    +I
Sbjct:   22 VQQLQWDTWKSTYGKHYGSEQEDAHRRDVFEQNLKRVLQHNLLADEGNVSFHLGINKYSDLELHEYHEKVVGRFWNLRNGTRRRG-APFPLRSMDNLPEQVDWRLKGYVTPVKEQGLCGSSWAFSATGSLEGQHFAATGNLTSLSEQQLVDCTKSYYNNGCNGGRSERALQYIIDNNGIDSELSYPYEHADGKCRFKPANVATKCSSYQFVEPSSNEEVLRQAVASVGPIAIAMNADLDTFKHYKSGLFNEPSCDKSPN---HAMLVVGYGS-LSGNDFWIVKNSWGEDWGEKGYIYMIRNKDNQCGIASIGIYPII 333          
BLAST of Cathepsin F vs. Ensembl Nematostella
Match: EDO42751 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0K3])

HSP 1 Score: 346.28 bits (887), Expect = 2.555e-116
Identity = 165/279 (59.14%), Postives = 204/279 (73.12%), Query Frame = 2
Query:  746 FKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIP-QDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
             KIF SN +KA  M ++D G+A YG T FSDL++EEFR Q + P +       + ++IP  DI   ++VDWR  G VT VKNQGSCGSCWAFSTTGN+EGQ+ +KT +LVSLSEQ+LVDCDTID GC GGLPS AYK I+++GGLESE  YPY      C  +K+++ V IN S  I   E  +A W + NGPISIGINA AMQFY GGI+HP+ IFCNP+ L+HGVLIVG+    +G P+WI+KNSWG SWGE GYYL+YRG G CGLN M T++VI+
Sbjct:    1 MKIFESNMRKAAKMQKMDSGTAQYGPTIFSDLSEEEFRKQKMMPGWGKPLYEMKDAEIPLGDI--PESVDWRDKGVVTPVKNQGSCGSCWAFSTTGNIEGQYAIKTGKLVSLSEQELVDCDTIDKGCEGGLPSNAYKQIEKLGGLESESDYPYKGADSKCKFNKAEVKVTINSSVVISKDEKEIAAWLAKNGPISIGINANAMQFYMGGIAHPWKIFCNPSSLNHGVLIVGYG-VKNGTPYWIIKNSWGPSWGEKGYYLIYRGGGCCGLNTMCTSAVID 276          
BLAST of Cathepsin F vs. Ensembl Nematostella
Match: EDO45953 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRH6])

HSP 1 Score: 210.69 bits (535), Expect = 2.576e-63
Identity = 119/303 (39.27%), Postives = 172/303 (56.77%), Query Frame = 2
Query:  689 SFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLS--PAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTI--DNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASI-DSSESFMANWCSVNGPISIGINAFAMQFYRGGI-SHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRG-SGVCGLNKMATTSVI 1576
            ++K    K Y +  EE  R  I+  N +K    N  D  S    +    DLT++EFR  YL     ++ +KR       P ++K   +VDW   G VT VKNQG CGSCWAFSTTG+VEGQ F KT  LVSLSEQ L+DC     +NGC GGL   A++ I+  GG+++E SYPY   +G+C    S +   + G   I   SE  + +  +  GP+S+ ++A   QFY  G+  +P   +C+   LDHGVL++G+ +  +G+ +W+VKNSWG SWG  GY ++ R  +  CG+   A+  ++
Sbjct:   29 AWKSYHGKSYSDVHEERTRMAIWQQNLEKIKRHNAEDH-SYKMAMNHLGDLTEDEFRYFYLGVRAHHNSTKRGWATYMPPSNVKIPSSVDWSQKGYVTGVKNQGQCGSCWAFSTTGSVEGQHFRKTGSLVSLSEQNLIDCSGSYGNNGCQGGLMDNAFRYIESNGGIDTESSYPYLGQQGSCHFSSSHVGARVTGYQDIPQGSEQALQSAVATVGPVSVAVDASQWQFYSSGVYDNP---YCSSTQLDHGVLVIGYGN-YNGQDYWLVKNSWGYSWGVEGYIMMSRNKNNQCGIASSASYPLV 326          
BLAST of Cathepsin F vs. Ensembl Nematostella
Match: EDO36727 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHX1])

HSP 1 Score: 204.912 bits (520), Expect = 5.234e-61
Identity = 123/307 (40.07%), Postives = 167/307 (54.40%), Query Frame = 2
Query:  683 FDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYL---SPAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTI--DNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASI-DSSESFMANWCSVNGPISIGINAFAM--QFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRG-SGVCGLNKMATTSVI 1576
            + ++K    K Y    EE  R  I+  N KK +  +  +  S    +    DLT +EFR  Y    S   + +K+       P  ++    VDWR  G VT VKNQG CGSCWAFSTTG++EGQ F KT +LVSLSEQ LVDC T   +NGC GGL   A+K I++ GG+++E+SYPY A    C   KS I     G   +    E  +       GPIS+ I+A  M  QFY  G+ +  +  C+   LDHGVL+VG+  T  G  +W+VKNSWGE WG  GY ++ R  +  CG+   A+  ++
Sbjct:   28 WQAWKLFHTKKYTTVTEEGARKAIWRDNLKK-IQKHNAEGHSFTLAMNHLGDLTQDEFRYFYTGMRSHYSNYTKKQGSAFLAPSHVQVPDTVDWRKEGYVTPVKNQGQCGSCWAFSTTGSLEGQNFKKTGKLVSLSEQNLVDCSTAYGNNGCQGGLMDYAFKYIKENGGIDTEESYPYEARNDRCRFQKSNIGAVDTGFVDVTHGDEEALKTAAGTVGPISVAIDAGHMSFQFYHSGVYN--NAGCSSTSLDHGVLVVGY-GTYQGSDYWLVKNSWGERWGMEGYIMMSRNKNNQCGVATQASYPLV 330          
BLAST of Cathepsin F vs. Ensembl Nematostella
Match: EDO45852 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRL9])

HSP 1 Score: 202.216 bits (513), Expect = 5.547e-60
Identity = 126/321 (39.25%), Postives = 169/321 (52.65%), Query Frame = 2
Query:  641 VLGLPDGEVVSGLNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQI---PQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTI--DNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDS-SESFMANWCSVNGPISIGINA--FAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGS-GVCGLNKMATTSVI 1576
             L +P  E +   N+ ++K    K Y N+ EE  R  I+ +N KK +  N   K S    +    D+T  E     L          Q        P ++K   ++DWR+ G VT VKNQG CGSCWAFSTTG +EGQ F KT +LVSLSEQ LVDC     +NGC GGL   A++ I++ GG+++E SYPY A  G C  +KS I     G   I +  E+ +    +  GPISI I+A      FY  G+       C+   LDHGVL VG+  T  G+ +W+VKNSWG SWGE GY  + R     CG+   A+  ++
Sbjct:   15 ALAMPPMEPLQDPNWKAWKSFHGKEYPNKNEETMRNFIWQNNLKKIVTHNE-GKHSFKLAMNHLGDMTSLEISQTLLGLKLKKHAESQPKGATFLPPANVKVVDSIDWRSKGYVTPVKNQGQCGSCWAFSTTGALEGQHFRKTGKLVSLSEQNLVDCSGKYGNNGCEGGLMDNAFQYIKENGGIDTEKSYPYLAKDGVCHYNKSAIGAKDTGFVDIPTGDENALQQALASVGPISIAIDASQSTFHFYHQGVYDDPD--CSSTRLDHGVLAVGY-GTDDGKDYWLVKNSWGPSWGEEGYIKIARNDHDKCGVASKASYPLV 331          
BLAST of Cathepsin F vs. Ensembl Nematostella
Match: EDO46465 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPY5])

HSP 1 Score: 189.504 bits (480), Expect = 2.443e-55
Identity = 120/314 (38.22%), Postives = 173/314 (55.10%), Query Frame = 2
Query:  662 EVVSGLNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQ-IP-QDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTI--DNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASID-SSESFMANWCSVNGPISIGINA--FAMQFYRGGI-SHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRG-SGVCGLNKMATTSVI 1576
            E+     + ++K    K Y  EEE+ +R  I++ N +     N  +  S    +  F+DLT  EF+ +++   Y  +      S  +P  +++    VDWR  G VTAVKNQG CGSCWAFS+TG++EGQ F KT +LVSLSEQ LVDC     +NGC GGL   A+K I+   G+++E SYPY A  G C      +   + G   +   SE  + +  +  GPIS+ I+A   + Q Y+ G+ S P    C+   LDHGVL VG+ +   G+ +W+VKNSWGE WG NGY  + R     CG+   A+  ++
Sbjct:   20 ELSQDRQWHAWKDFHGKTYTGEEEDLRR-AIWNDNLEIVKKHN-AENHSYKLDMNHFADLTVTEFKQRFM--GYRAASNSTGGSTFLPLSNVQLPAEVDWRDKGFVTAVKNQGQCGSCWAFSSTGSLEGQHFRKTGKLVSLSEQNLVDCSKKYGNNGCEGGLMDYAFKYIKNNDGIDTEQSYPYTARDGQCHFKPGSVGATVTGYTDVQRGSEGDLQSAVATVGPISVAIDAGHSSFQLYKTGVYSEP---DCSSTQLDHGVLAVGYGA-EDGKDYWLVKNSWGEGWGMNGYIKMSRNKDNQCGIATQASYPLV 325          
BLAST of Cathepsin F vs. Ensembl Medaka
Match: ctsf (cathepsin L [Source:NCBI gene;Acc:101161416])

HSP 1 Score: 343.969 bits (881), Expect = 4.120e-112
Identity = 185/440 (42.05%), Postives = 251/440 (57.05%), Query Frame = 2
Query:  302 DKDLQEVFREAINKYNSRVNSIYSYAPYQVHSIRQQVVAGILYEFEVTLVGTNCR-NELKRSNQLLSECGLNENLAPKRAKLQIVSTPW----------LEPKHHFEVMSEIEEL--DLKLHDQLFVLGLPDGEVVSGLNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAY-DLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVIN 1579
            D  L++V   A  +YN   N+++     +  S  +Q+V GI Y   V L  T C+ + + R+     E    + L  +    ++   PW            PK   E  + + E      + + + +LG           F  F   + K Y ++EE  +R +IF  N K A  +  LD+GSA YGVT+FSDLT+EEFRS YL+P     +         P       + DWR  G V+ VKNQG CGSCWAFS TGN+EGQWFLK   L+SLSEQ+LVDCD +D  C GGLPS AY+AI+++GGLESE  Y Y  +K  C     K+A YIN S  +   E  +A W + NGPIS+ +NAFAMQFY+ G+SHP+ IFCNP  +DH VL+VG+    +G PFW +KNSWGE +GE GYY + RGS  CG+N+M +++VIN
Sbjct:   48 DPGLKKVMLFAEERYNRGSNAMHLRKISRFVSATKQLVKGIRYTITVELSNTLCKKSAMVRTCDFYPE---TQKLKTEVCVFEVWDIPWQGTSTLLKQKCSPKAEVEETNRVAEPTNSQPVEESVQLLG----------QFKDFMVKYKKDYSSQEEAERRLQIFQENLKTAEKLQALDQGSAEYGVTKFSDLTEEEFRSTYLNPLLSQWTLHRGMKPAPPAKTPAPDSWDWRDHGAVSPVKNQGMCGSCWAFSVTGNIEGQWFLKNGTLLSLSEQELVDCDGLDQACRGGLPSNAYEAIEKLGGLESETDYSYTGHKQKCDFTNRKVAAYINSSVELPKDEREIAAWLAENGPISVALNAFAMQFYKKGVSHPWKIFCNPWMIDHAVLLVGYGE-RNGIPFWAIKNSWGEDYGEQGYYYLQRGSNACGINRMGSSAVIN 473          
BLAST of Cathepsin F vs. Ensembl Medaka
Match: ctsl.1 (cathepsin L1 [Source:NCBI gene;Acc:101171789])

HSP 1 Score: 209.534 bits (532), Expect = 3.120e-62
Identity = 124/314 (39.49%), Postives = 183/314 (58.28%), Query Frame = 2
Query:  677 LNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRL-DKGSAVY--GVTQFSDLTDEEFR---SQYLSPAYD--LSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDC--DTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDS-SESFMANWCSVNGPISIGINA--FAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRG-SGVCGLNKMATTSVI 1576
            L F +++  F + Y +  EEA+R + + +N K  L+ N L D+G   Y  G+T F+D+ +EE++   SQ    +++  L +R     ++P++     AVDWR  G VT VK+Q  CGSCWAFS TG++EGQ F KT +LVSLSEQQLVDC  D  + GC GGL   A++ IQ  GG+++E+SYPY A  G C      +     G   + S  E  +    +  GPIS+GI+A   + Q Y  G+       C+ + LDHGVL VG+ S  +G+ +W+VKNSWG +WG+ GY  + +  S  CG+   A+  ++
Sbjct:   29 LEFHAWRLKFGRTYSSPTEEAQRRQTWLNNRKLVLVHNILADQGIKSYRLGMTYFADMENEEYKRLISQGCLGSFNASLPRRGSTFFRLPENKDLPAAVDWRDKGYVTDVKDQKQCGSCWAFSATGSLEGQTFRKTGKLVSLSEQQLVDCSGDYGNMGCGGGLMDDAFRYIQATGGIDTEESYPYEAEDGECRYKPDAVGATCTGYVDVSSGDEDALQEAVATIGPISVGIDASHISFQLYESGLYDEPQ--CSSSELDHGVLAVGYGS-ENGQDYWLVKNSWGLTWGDQGYIKMSKNKSNQCGIATAASYPLV 339          
BLAST of Cathepsin F vs. Ensembl Medaka
Match: ctsk (cathepsin K [Source:NCBI gene;Acc:101164922])

HSP 1 Score: 202.986 bits (515), Expect = 7.653e-60
Identity = 127/341 (37.24%), Postives = 178/341 (52.20%), Query Frame = 2
Query:  590 VMSEIEELDLKLHDQLFVLGLPDGEVVSGLNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNR---LDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQD-----IKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASI-DSSESFMANWCSVNGPISIGINAFAMQF--YRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSG-VCGLNKMATTSVI 1576
            VMS+++E  L  H                  ++ +K    K Y   EEE  R  I+  N +     N+   L   +   G+ QF D+T EE   +       L+  P+    +P +     IK  ++VD+R  G VT+VKNQGSCGSCWAFS+ G +EGQ   KT  LV LS Q LVDC T ++GC GG  + A+K +Q+ GG++SE +YPY      C  + S +A  I G   + +  E  +A      GP+S+GI+A    F  Y+ GI   F   CN   ++H VL VG+     G+ FWIVKNSWGE+WG  GY L+ R  G VCG+  +A+  V+
Sbjct:   15 VMSQMDETTLDAH------------------WEEWKMTHTKEYITVEEEGIRRAIWEKNLRMIEAHNQEAALGMHTYTLGMNQFGDMTQEEVVERMTGLQMPLNPEPR----VPMETDGSLIKLPKSVDYRKKGMVTSVKNQGSCGSCWAFSSVGALEGQLAKKTGNLVDLSPQNLVDCVTENDGCGGGYMTNAFKYVQENGGIDSEAAYPYMGEDQPCRYNVSGLAAQIKGYKEVPEGDEHALAVALFKAGPVSVGIDASQNSFLYYQKGIY--FDRNCNKEDINHAVLAVGYGVNAKGKKFWIVKNSWGETWGNKGYVLMARNRGNVCGIANLASYPVM 331          
BLAST of Cathepsin F vs. Ensembl Medaka
Match: ctsl (cathepsin L [Source:NCBI gene;Acc:100049246])

HSP 1 Score: 192.971 bits (489), Expect = 5.424e-56
Identity = 124/304 (40.79%), Postives = 168/304 (55.26%), Query Frame = 2
Query:  722 NEEEEAKRFKIFHSNYKKALLMN---RLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQR-----ISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTID--NGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGN-CALDKSKIAVYINGSASIDSSE--SFMANWCSVNGPISIGINAF--AMQFYRGGISHPFSIFCNPNHLDHGVLIVGF---NSTTSGEPFWIVKNSWGESWGENGY-YLVYRGSGVCGLNKMATTSVI 1576
            +E+EE  R  ++  N +K  L N    + K S   G+  F D+T EEFR   +   Y   +R QR     +   P  ++  +AVDWR  G VT VK+QG CGSCWAFSTTG +EGQ F KT +LVSLSEQ LVDC   +   GCNGGL  QA++ ++   GL+SED YPY       C  +    AV   G   I S +  + M    SV GP+S+ I+A   + QFY+ GI   F   C+ + LDHGVL+VG+        G+ +WIVKNSW E WG+ G+ Y+       CG+   A+  ++
Sbjct:   40 HEKEEGWRRLVWEKNLRKIELHNLEHSMGKHSYRLGMNHFGDMTHEEFRQ--IMNGY--KRREQRKYSGSLFMEPNFLEAPRAVDWRDKGYVTPVKDQGQCGSCWAFSTTGALEGQQFRKTGKLVSLSEQNLVDCSRPEGNEGCNGGLMDQAFQYVKDNQGLDSEDFYPYKGTDDQPCQYNAQYSAVNDTGFVDIPSGKERALMKAVASV-GPVSVAIDAGHESFQFYQSGIY--FEKECSSDELDHGVLVVGYGFEGEDVDGKKYWIVKNSWSEKWGDKGFIYMAKDRHNHCGIATAASYPLV 336          
BLAST of Cathepsin F vs. Ensembl Medaka
Match: ctsh (cathepsin H [Source:NCBI gene;Acc:111947539])

HSP 1 Score: 181.415 bits (459), Expect = 7.059e-52
Identity = 109/294 (37.07%), Postives = 160/294 (54.42%), Query Frame = 2
Query:  731 EEAKRFKIFHSNYKKALLMNRLDKG--SAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQ-----AVDWRALGG-VTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDC--DTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDS-SESFMANWCSVNGPISIGINAFA-MQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVI 1576
            E  +R +IF  N K   L+++ ++G  S    + QFSD+T  EFR  +L         PQ  S    +  +++     ++DWR  G  +T VKNQG+CGSCW FSTTG +E    + T +L+SLSEQQLVDC  D  ++GCNGGLPSQA++ I    GL +E  YPY A +  C    S  A ++    ++ +  E  M +  + + P+S      +    Y  G+          + ++H VL VG+    +G P+WIVKNSWG  WG +GY+L+ RG  +CGL   A+  V+
Sbjct:   41 EYHQRLQIFTENKK---LIDKHNEGNHSFTMALNQFSDMTFTEFRKAFLW------SEPQNCSATSGNHLSSKGPYPDSIDWRKKGNYITPVKNQGACGSCWTFSTTGCLESVTAIATGKLLSLSEQQLVDCAQDFNNHGCNGGLPSQAFEYIMYNKGLMTEQDYPYTAMEDKCMYKPSLAAAFVKEVVNVTAYDEMGMVDAVATHNPVSFAFEVTSDFMNYHQGVYTSTECHSTADKVNHAVLAVGYGQ-ENGTPYWIVKNSWGSKWGMDGYFLIERGKNMCGLAACASFPVV 324          
BLAST of Cathepsin F vs. Planmine SMEST
Match: SMESG000005279.1 (SMESG000005279.1)

HSP 1 Score: 948.347 bits (2450), Expect = 0.000e+0
Identity = 456/459 (99.35%), Postives = 458/459 (99.78%), Query Frame = 2
Query:  209 QTSTLFLFLINVGLLFGTGEKREISLSKLRKDKDLQEVFREAINKYNSRVNSIYSYAPYQVHSIRQQVVAGILYEFEVTLVGTNCRNELKRSNQLLSECGLNENLAPKRAKLQIVSTPWLEPKHHFEVMSEIEELDLKLHDQLFVLGLPDGEVVSGLNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVINLN 1585
            QTSTLFLFLINVGLLFGTGEKREISLSKLRKDKDLQEVFREAINKYNSRVNSIYSYAPYQVHSIRQQVVAGILYEFEVTLVGTNCRNELKRSNQLLSECGLNENLAPKRAKLQIVSTPWLEPKHHFEVMSEIEELDLKLHDQLFVLGLPDGEVVSGLNFDSFKK FNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQAVDWRALGGV+AVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFY+GGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVINLN
Sbjct:    3 QTSTLFLFLINVGLLFGTGEKREISLSKLRKDKDLQEVFREAINKYNSRVNSIYSYAPYQVHSIRQQVVAGILYEFEVTLVGTNCRNELKRSNQLLSECGLNENLAPKRAKLQIVSTPWLEPKHHFEVMSEIEELDLKLHDQLFVLGLPDGEVVSGLNFDSFKKKFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVSAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGINAFAMQFYQGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLNKMATTSVINLN 461          
BLAST of Cathepsin F vs. Planmine SMEST
Match: SMESG000049781.1 (SMESG000049781.1)

HSP 1 Score: 211.46 bits (537), Expect = 3.513e-63
Identity = 122/301 (40.53%), Postives = 169/301 (56.15%), Query Frame = 2
Query:  692 FKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVY----GVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGIN-AFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGY-YLVYRGSGVCGLNKMATTSVI 1576
            + K +NK Y+N   E  R  I+  N K  +  + LD    +Y    G+ QF+DLT  EF++ YLS     +K                 VDW   G VT VK+QG CGSCWAFS TG++EGQ+F KT RLVS SEQQLVDC   +NGC+GGL + A+  I+ +GG+ESE  YPY A + NC  + SK+   ++G   ID +E  +A   +  GPIS+GI+ +   Q Y+ G+ +       PN   HGVL+VG+  T+  + +WIVKNSWG  WG  GY ++       C +   A+  V+
Sbjct:   31 YMKIYNKTYENPFHELIRRMIWEDNLK-YIHKHNLDHDLGLYTFTLGINQFADLTHAEFKAIYLSGNLKPTKTGNTYLPPLNAFNVPANVDWTEKGYVTDVKDQGKCGSCWAFSATGSLEGQFFRKTGRLVSFSEQQLVDCSKSNNGCHGGLMTSAFNDIKDLGGIESEQEYPYKAQQLNCEFNHSKVVTSVSGYKEIDENEGSLAIAVANEGPISVGISVSLNFQLYKSGVYNEPDCSRVPN---HGVLVVGY-GTSVNDNYWIVKNSWGPLWGMKGYIWMSKDKDNQCAIASFASFPVV 326          
BLAST of Cathepsin F vs. Planmine SMEST
Match: SMESG000049723.1 (SMESG000049723.1)

HSP 1 Score: 211.075 bits (536), Expect = 4.443e-63
Identity = 127/310 (40.97%), Postives = 186/310 (60.00%), Query Frame = 2
Query:  683 FDSFKKNFNKLYQNEEEEAKRFKIFHSNYK---KALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQI--PQDIKT-AQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTI--DNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASI-DSSESFMANWCSVNGPISIGINA--FAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSG-VCGLNKMATTSVI 1576
            ++++K  F K Y +  E ++R  I+  N K   +  L + L K +   G+  F+D+T+EEFR+ YL  +    KR    S    P+++     +VDWR  G VT VKNQ  CGSCW+FSTTG++EGQ + KT+ L+S SEQQLVDC     +NGC+GGL   A++ IQQ  G+E+E +YPY A  GNC  DKSK+     G + I   +E  +A+  +  GPIS+ I+A  ++ Q Y+ GI +     C+ + LDHGVL +G+  T +G+ FW+VKNSWG  WG  GY  + +  G  CG+  MA+  ++
Sbjct:   31 WEAYKTQFGKKYVSLNEISRRL-IWEQNLKYIQQHNLEHDLGKHTYTLGLNHFADMTNEEFRANYLGVS---PKRMNFTSHFIAPENVGALPSSVDWRTKGFVTPVKNQQQCGSCWSFSTTGSLEGQHYRKTQNLISFSEQQLVDCSGSFGNNGCDGGLMDSAFQYIQQY-GIEAESTYPYTAQDGNCVYDKSKVVGSCTGFSDIPHGNEQALASAVATVGPISVAIDASNYSFQLYKSGIYNEPQ--CSSSQLDHGVLAIGY-GTENGKNFWLVKNSWGIGWGIQGYIKMSKDQGNQCGIATMASYPLV 332          
BLAST of Cathepsin F vs. Planmine SMEST
Match: SMESG000049783.1 (SMESG000049783.1)

HSP 1 Score: 208.764 bits (530), Expect = 3.355e-62
Identity = 119/279 (42.65%), Postives = 161/279 (57.71%), Query Frame = 2
Query:  692 FKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDKGSAVY----GVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDSSESFMANWCSVNGPISIGIN-AFAMQFYRGGISHPFSIFCNPNHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGY 1513
            + K +NK Y+N   E  R  I+  N K  +  + LD    +Y    G+ QF+DLT  EF++ YLS     +K                 VDW   G VT VK+QG+CGSCWAFSTTG++EGQ+F KT RLVS SEQQLVDC   +NGCNGGL + A+  I+ +GG+ESE  YPY A + NC  ++SK+   ++G   I  +E  +A   +  GPIS+ I  +   Q Y+ G+ +       PN   H VL+VG+  T+  + +WIVKNSWG SWG  GY
Sbjct:   31 YMKIYNKTYENPFHELIRRMIWEDNLK-YIHKHNLDHDLGLYTFTLGINQFADLTHAEFKAIYLSGNLRPTKTGNTYLPPLNAFNVPANVDWTEKGYVTDVKDQGNCGSCWAFSTTGSLEGQFFRKTGRLVSFSEQQLVDCSKSNNGCNGGLMTSAFNDIKDLGGIESEQEYPYQAQQLNCVFNRSKVVTSVSGYKEIYKNEGSLAIAVANEGPISVLIRVSLNFQLYKSGVYNEPDCSRVPN---HAVLVVGY-GTSVNDNYWIVKNSWGPSWGMKGY 304          
BLAST of Cathepsin F vs. Planmine SMEST
Match: SMESG000049716.1 (SMESG000049716.1)

HSP 1 Score: 206.068 bits (523), Expect = 4.108e-61
Identity = 129/303 (42.57%), Postives = 174/303 (57.43%), Query Frame = 2
Query:  692 FKKNFNKLYQNEEEEAKRFKIFHSNYK---KALLMNRLDKGSAVYGVTQFSDLTDEEFRSQYLS--PAYDLSKRPQRISQIPQDIKTAQAVDWRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDCDTI--DNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVYINGSASIDS-SESFMANWCSVNGPISIGINAF--AMQFYRGGISHPFSIFCNPNHLDHGVLIVGFN-STTSGEPFWIVKNSWGESWGENGYYLVYRG-SGVCGLNKMAT 1564
            +K  FNK+Y +EE E  R +I+  N +   K  +   L +     GV QF+DL+ +EF   Y+   P     +R +   +  + +K   +V+W   G VT VKNQ  CGSCWAFSTTG++EGQ+F KT++LVS SEQQLVDC     + GCNGG   QA++ I +  G+E E  YPY A  GNC   K K+         ID  SE  +A   +  GPIS+GI+A   + Q Y GGI    S  C+   LDHGVL VG+     SGE +W+VKNSWG+SWG NGY  + +  +  CG+  MA+
Sbjct:   35 YKTKFNKVY-DEENEFVRRQIWEENLQFIQKHNIEYNLGRHKYTLGVNQFTDLSHQEFLDIYMKYRPIVKSVER-KSFDKKRKSLKLPNSVNWVKKGYVTPVKNQLQCGSCWAFSTTGSMEGQYFKKTRKLVSFSEQQLVDCSQSYGNEGCNGGFMDQAFQYIMKF-GIEKESVYPYTAMDGNCQYSKRKVITRCKNYTDIDQGSEEDLAEKLATVGPISVGIDASNPSFQSYNGGIYDEES--CSSTQLDHGVLAVGYGVDEDSGEKYWLVKNSWGDSWGLNGYIKMSKDKNNQCGIATMAS 332          
The following BLAST results are available for this feature:
BLAST of Cathepsin F vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CTSF6.422e-9349.51cathepsin F [Source:HGNC Symbol;Acc:HGNC:2531][more]
CTSF3.224e-6948.44cathepsin F [Source:HGNC Symbol;Acc:HGNC:2531][more]
CTSL1.919e-5740.52cathepsin L [Source:HGNC Symbol;Acc:HGNC:2537][more]
CTSL1.919e-5740.52cathepsin L [Source:HGNC Symbol;Acc:HGNC:2537][more]
CTSW2.064e-5532.82cathepsin W [Source:HGNC Symbol;Acc:HGNC:2546][more]
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BLAST of Cathepsin F vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tag-1962.944e-11140.97pep chromosome:WBcel235:V:8594914:8597500:1 gene:W... [more]
tag-1962.944e-11140.97pep chromosome:WBcel235:V:8594914:8597469:1 gene:W... [more]
R09F10.11.035e-5637.58pep chromosome:WBcel235:X:8323592:8325046:1 gene:W... [more]
R07E3.13.713e-5134.32pep chromosome:WBcel235:X:10339226:10341095:-1 gen... [more]
R07E3.13.713e-5134.32pep chromosome:WBcel235:X:10339226:10340968:-1 gen... [more]
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BLAST of Cathepsin F vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG121632.002e-8938.88gene:FBgn0260462 transcript:FBtr0078822[more]
CG121636.831e-8845.51gene:FBgn0260462 transcript:FBtr0078823[more]
Cp18.435e-6539.94gene:FBgn0013770 transcript:FBtr0087592[more]
Cp11.087e-6439.94gene:FBgn0013770 transcript:FBtr0087593[more]
Cp19.342e-6440.20gene:FBgn0013770 transcript:FBtr0339955[more]
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BLAST of Cathepsin F vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ctsf1.705e-11341.69cathepsin F [Source:ZFIN;Acc:ZDB-GENE-030131-9831][more]
ctsf1.705e-11341.69cathepsin F [Source:ZFIN;Acc:ZDB-GENE-030131-9831][more]
ctsl.17.851e-6039.49cathepsin L.1 [Source:ZFIN;Acc:ZDB-GENE-040718-61][more]
ctsl.17.851e-6039.49cathepsin L.1 [Source:ZFIN;Acc:ZDB-GENE-040718-61][more]
ctsl.17.851e-6039.49cathepsin L.1 [Source:ZFIN;Acc:ZDB-GENE-040718-61][more]
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BLAST of Cathepsin F vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
klhl313.540e-12543.47kelch like family member 31 [Source:Xenbase;Acc:XB... [more]
klhl318.024e-12543.47kelch like family member 31 [Source:Xenbase;Acc:XB... [more]
klhl311.064e-11254.74kelch like family member 31 [Source:Xenbase;Acc:XB... [more]
klhl312.701e-11054.80kelch like family member 31 [Source:Xenbase;Acc:XB... [more]
klhl311.499e-10940.54kelch like family member 31 [Source:Xenbase;Acc:XB... [more]
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BLAST of Cathepsin F vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ctsf4.590e-9550.00cathepsin F [Source:MGI Symbol;Acc:MGI:1861434][more]
Ctsw3.226e-6233.85cathepsin W [Source:MGI Symbol;Acc:MGI:1338045][more]
Ctsl3.033e-5937.50cathepsin L [Source:MGI Symbol;Acc:MGI:88564][more]
Ctsl3.033e-5937.50cathepsin L [Source:MGI Symbol;Acc:MGI:88564][more]
Ctsll37.949e-5637.13cathepsin L-like 3 [Source:MGI Symbol;Acc:MGI:1917... [more]
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BLAST of Cathepsin F vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q26534|CATL_SCHMA8.897e-10552.86Cathepsin L OS=Schistosoma mansoni OX=6183 GN=CL1 ... [more]
sp|Q9R013|CATF_MOUSE3.211e-9450.00Cathepsin F OS=Mus musculus OX=10090 GN=Ctsf PE=1 ... [more]
sp|Q9UBX1|CATF_HUMAN3.084e-9249.51Cathepsin F OS=Homo sapiens OX=9606 GN=CTSF PE=1 S... [more]
sp|Q9VN93|CPR1_DROME6.847e-8745.51Putative cysteine proteinase CG12163 OS=Drosophila... [more]
sp|Q8VYS0|RD19D_ARATH2.743e-8144.51Probable cysteine protease RD19D OS=Arabidopsis th... [more]
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BLAST of Cathepsin F vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267DEW17.241e-14347.88Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FPA59.496e-14347.88Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FSZ76.475e-14249.42Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8IVL06.414e-13648.73Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8I5R82.238e-13449.65Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Cathepsin F vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ctsf9.037e-11342.82cathepsin F [Source:NCBI gene;Acc:103042155][more]
ctsf4.317e-11242.26cathepsin F [Source:NCBI gene;Acc:103042155][more]
ENSAMXT00000001970.21.273e-6139.30cathepsin L1-like [Source:NCBI gene;Acc:103022163][more]
ctsl.17.729e-6039.30cathepsin L1-like [Source:NCBI gene;Acc:103023607][more]
ENSAMXT00000001927.21.129e-5837.38cathepsin L1-like [Source:NCBI gene;Acc:107197646][more]
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BLAST of Cathepsin F vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ctsf1.876e-10351.37cathepsin F [Source:ZFIN;Acc:ZDB-GENE-030131-9831][more]
ENSPMAT00000011285.19.368e-6041.86pep scaffold:Pmarinus_7.0:GL499136:454:1347:-1 gen... [more]
ENSPMAT00000010475.19.748e-5939.46pep scaffold:Pmarinus_7.0:GL482653:10321:33550:-1 ... [more]
ENSPMAT00000006499.13.538e-5637.97pep scaffold:Pmarinus_7.0:GL477089:42202:47941:1 g... [more]
ENSPMAT00000007755.16.035e-5637.85pep scaffold:Pmarinus_7.0:GL476381:1843:17023:-1 g... [more]
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BLAST of Cathepsin F vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cathepsin F vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO427512.555e-11659.14Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO459532.576e-6339.27Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO367275.234e-6140.07Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO458525.547e-6039.25Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO464652.443e-5538.22Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Cathepsin F vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ctsf4.120e-11242.05cathepsin L [Source:NCBI gene;Acc:101161416][more]
ctsl.13.120e-6239.49cathepsin L1 [Source:NCBI gene;Acc:101171789][more]
ctsk7.653e-6037.24cathepsin K [Source:NCBI gene;Acc:101164922][more]
ctsl5.424e-5640.79cathepsin L [Source:NCBI gene;Acc:100049246][more]
ctsh7.059e-5237.07cathepsin H [Source:NCBI gene;Acc:111947539][more]
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BLAST of Cathepsin F vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000005279.10.000e+099.35SMESG000005279.1[more]
SMESG000049781.13.513e-6340.53SMESG000049781.1[more]
SMESG000049723.14.443e-6340.97SMESG000049723.1[more]
SMESG000049783.13.355e-6242.65SMESG000049783.1[more]
SMESG000049716.14.108e-6142.57SMESG000049716.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027540 ID=SMED30027540|Name=Cathepsin F|organism=Schmidtea mediterranea sexual|type=transcript|length=1759bp
GATATGATTGCAGACAAATATCTTTAGTGTGGTCTTACTTCATTCTTAAA
GCTATTTTTCATTCTGTTGTTATCTCATAGATACCAATTAAATTAGTAAT
TATTATTGTGCATACTAAAAATTAACTAGGTCAAAGTTCAATCTCAATCA
AATTATGAAAACTTATATAACTTGAATTATTACTTTAATTAATCATTTGA
ATATGATACAAACAAGCACTTTATTTTTATTTCTGATAAATGTTGGTTTG
TTATTCGGTACAGGTGAAAAACGTGAAATTAGTTTGTCGAAGCTAAGAAA
AGATAAAGATTTGCAAGAAGTTTTCAGAGAGGCAATTAACAAATACAATA
GCAGAGTGAATAGTATTTATTCTTATGCACCTTATCAAGTTCACAGTATA
CGTCAACAGGTCGTTGCTGGGATATTATATGAGTTCGAAGTCACATTGGT
TGGTACAAATTGTCGCAATGAACTAAAGAGATCCAATCAACTTTTATCTG
AATGTGGATTAAATGAAAATCTTGCGCCAAAACGAGCTAAATTACAAATC
GTCTCAACTCCATGGCTAGAACCGAAACACCATTTTGAAGTAATGAGTGA
AATTGAAGAGCTTGATTTAAAATTGCACGATCAGCTGTTTGTACTTGGAT
TACCCGACGGTGAAGTCGTTAGCGGTTTGAATTTTGATTCATTTAAAAAA
AATTTCAATAAATTATATCAAAATGAAGAAGAAGAAGCTAAGCGATTCAA
GATCTTCCACTCAAATTACAAAAAAGCATTACTGATGAATCGGCTAGATA
AAGGGTCCGCTGTATATGGGGTAACTCAATTTTCCGATTTAACGGATGAG
GAATTTAGAAGTCAATATCTTAGCCCGGCATATGATTTATCGAAAAGGCC
ACAGAGAATATCCCAAATACCGCAAGACATTAAAACAGCACAAGCTGTTG
ATTGGCGGGCCCTAGGAGGTGTCACAGCAGTAAAGAATCAGGGATCATGT
GGATCATGTTGGGCATTTTCAACGACTGGAAATGTTGAAGGTCAATGGTT
TTTGAAAACTAAACGATTAGTTTCCTTGAGTGAACAACAACTGGTAGATT
GTGATACTATTGATAACGGATGCAACGGGGGCCTTCCAAGCCAGGCATAC
AAGGCTATCCAGCAAATGGGGGGTTTAGAATCTGAGGACAGTTATCCCTA
TAATGCAAATAAAGGAAATTGTGCATTAGATAAATCAAAAATAGCTGTTT
ATATAAATGGATCTGCATCAATTGATTCATCAGAATCATTTATGGCAAAT
TGGTGCTCTGTTAATGGACCAATAAGTATTGGAATCAACGCATTTGCAAT
GCAATTTTACCGAGGAGGCATTTCTCATCCATTTTCAATTTTTTGCAATC
CAAATCATTTGGATCATGGAGTACTTATAGTTGGATTCAATAGCACAACT
AGTGGTGAGCCATTTTGGATAGTTAAAAACAGCTGGGGAGAAAGCTGGGG
AGAAAATGGCTACTATCTAGTCTATCGAGGCTCGGGAGTTTGTGGTCTAA
ACAAAATGGCAACAACCTCCGTTATCAACCTCAACTAACCGAACGCCAAA
TATATTGTTTATAATTAACTTGTTGAAAAACTTTATTCAAATGTTTAACC
GATGGTGAAATTTTGATTAATTACTCACGTCTTCCTTATGAATTTGAATT
ATAAAAAGTTCAAGATTTAATTTTAATTGAAATAAATGAGTGTAATAAAA
CATCAAAAA
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protein sequence of SMED30027540-orf-1

>SMED30027540-orf-1 ID=SMED30027540-orf-1|Name=SMED30027540-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=462bp
MIQTSTLFLFLINVGLLFGTGEKREISLSKLRKDKDLQEVFREAINKYNS
RVNSIYSYAPYQVHSIRQQVVAGILYEFEVTLVGTNCRNELKRSNQLLSE
CGLNENLAPKRAKLQIVSTPWLEPKHHFEVMSEIEELDLKLHDQLFVLGL
PDGEVVSGLNFDSFKKNFNKLYQNEEEEAKRFKIFHSNYKKALLMNRLDK
GSAVYGVTQFSDLTDEEFRSQYLSPAYDLSKRPQRISQIPQDIKTAQAVD
WRALGGVTAVKNQGSCGSCWAFSTTGNVEGQWFLKTKRLVSLSEQQLVDC
DTIDNGCNGGLPSQAYKAIQQMGGLESEDSYPYNANKGNCALDKSKIAVY
INGSASIDSSESFMANWCSVNGPISIGINAFAMQFYRGGISHPFSIFCNP
NHLDHGVLIVGFNSTTSGEPFWIVKNSWGESWGENGYYLVYRGSGVCGLN
KMATTSVINLN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000067goblet cell
PLANA:0000070intestinal phagocyte
PLANA:0002056medial muscle cell
PLANA:0002075pigment cell
PLANA:0003116parenchymal cell
PLANA:0007528glial cell
Vocabulary: INTERPRO
TermDefinition
IPR039417Peptidase_C1A_papain-like
IPR038765Papain-like_cys_pep_sf
IPR000668Peptidase_C1A_C
IPR000010Cystatin_dom
IPR013201Prot_inhib_I29
IPR025661Pept_asp_AS
IPR000169Pept_cys_AS
IPR025660Pept_his_AS
Vocabulary: molecular function
TermDefinition
GO:0008234cysteine-type peptidase activity
GO:0004869cysteine-type endopeptidase inhibitor activity
GO:0008233peptidase activity
GO:0016787hydrolase activity
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
GO:0010951negative regulation of endopeptidase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 405..415
score: 45.71
coord: 421..427
score: 67.94
coord: 263..278
score: 64.89
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 245..457
e-value: 1.4E-90
score: 316.9
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 247..455
e-value: 2.4E-67
score: 227.2
IPR000010Cystatin domainSMARTSM00043CY_4coord: 24..133
e-value: 1.0E-4
score: 31.7
IPR000010Cystatin domainPFAMPF00031Cystatincoord: 33..116
e-value: 1.6E-11
score: 44.3
IPR000010Cystatin domainCDDcd00042CYcoord: 34..125
e-value: 2.5301E-10
score: 55.558
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 161..218
e-value: 4.0E-20
score: 82.8
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 161..218
e-value: 2.8E-9
score: 37.2
NoneNo IPR availableGENE3DG3DSA:3.90.70.10coord: 148..459
e-value: 5.2E-96
score: 324.0
NoneNo IPR availableGENE3DG3DSA:3.10.450.10coord: 20..133
e-value: 2.2E-17
score: 65.2
NoneNo IPR availablePANTHERPTHR12411:SF316CATHEPSIN Fcoord: 160..454
NoneNo IPR availablePANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 160..454
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..19
score: 0.696
NoneNo IPR availableSUPERFAMILYSSF54403Cystatin/monellincoord: 33..126
IPR025660Cysteine peptidase, histidine active sitePROSITEPS00639THIOL_PROTEASE_HIScoord: 403..413
IPR025661Cysteine peptidase, asparagine active sitePROSITEPS00640THIOL_PROTEASE_ASNcoord: 421..440
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 263..274
IPR039417Papain-like cysteine endopeptidaseCDDcd02248Peptidase_C1Acoord: 247..454
e-value: 5.83812E-99
score: 293.761
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILYSSF54001Cysteine proteinasescoord: 160..455