Calbindin-32
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30027507 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of Calbindin-32 vs. Ensembl Human
Match: CALB2 (calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435]) HSP 1 Score: 199.134 bits (505), Expect = 2.081e-56 Identity = 106/230 (46.09%), Postives = 153/230 (66.52%), Query Frame = 2
Query: 4358 QLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSA-FQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
L + TA+QFL++W HFD D NGYIE EL+NF + + G ++S+S F + F++ YD+N DG+IE+ EL+QILPT+E FL+ F++ + SS EFM+ W+++D DRSG+IEANEL+ F+++LLK K +E K+ +T +L++FD N DGKL LSEM+RLLP++EN+L K K++ E IF YD DR+G++DE E+ ALL DL E
Sbjct: 12 HLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARK----GSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLK---KANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK---FQGMKLTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYE 229 HSP 2 Score: 86.2705 bits (212), Expect = 1.636e-17 Identity = 50/137 (36.50%), Postives = 77/137 (56.20%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFL---IQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQK 4774
++ +F++ W +DTD +GYIEA EL FL ++ A EP Q QT L +D N DG++ + E+S++LP E FL+ FQ +S EF I+ +DKDRSG+I+ +EL + +L + N+K
Sbjct: 108 SSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEP---------KLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQGMK--LTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYEKNKK 233
BLAST of Calbindin-32 vs. Ensembl Human
Match: CALB2 (calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435]) HSP 1 Score: 199.134 bits (505), Expect = 2.081e-56 Identity = 106/230 (46.09%), Postives = 153/230 (66.52%), Query Frame = 2
Query: 4358 QLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSA-FQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
L + TA+QFL++W HFD D NGYIE EL+NF + + G ++S+S F + F++ YD+N DG+IE+ EL+QILPT+E FL+ F++ + SS EFM+ W+++D DRSG+IEANEL+ F+++LLK K +E K+ +T +L++FD N DGKL LSEM+RLLP++EN+L K K++ E IF YD DR+G++DE E+ ALL DL E
Sbjct: 12 HLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARK----GSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLK---KANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK---FQGMKLTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYE 229 HSP 2 Score: 86.2705 bits (212), Expect = 1.636e-17 Identity = 50/137 (36.50%), Postives = 77/137 (56.20%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFL---IQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQK 4774
++ +F++ W +DTD +GYIEA EL FL ++ A EP Q QT L +D N DG++ + E+S++LP E FL+ FQ +S EF I+ +DKDRSG+I+ +EL + +L + N+K
Sbjct: 108 SSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEP---------KLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQGMK--LTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYEKNKK 233
BLAST of Calbindin-32 vs. Ensembl Human
Match: CALB1 (calbindin 1 [Source:HGNC Symbol;Acc:HGNC:1434]) HSP 1 Score: 182.185 bits (461), Expect = 1.205e-50 Identity = 99/233 (42.49%), Postives = 150/233 (64.38%), Query Frame = 2
Query: 4346 ENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
E++ Q + TA+QF ++W HFD D +GY+E EL N + + + + G E+ + +TF++ Y + +DG+I I EL+ +LPT+E FL+LF R + S EFMK W+++D D SGFIE EL+ F+ +LL+ K V+++K+ +T++MLKLFD N+DGKL+L+EMARLLP++EN+L K K+ E + F+ YD D NG++DE E+ ALL DL E
Sbjct: 3 ESHLQSSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELQQA--RKKAGLELSPEM------KTFVDQYGQRDDGKIGIVELAHVLPTEENFLLLF-RCQQLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKANKT---VDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKF---QGIKMCGKEFNKAFELYDQDGNGYIDENELDALLKDLCE 220 HSP 2 Score: 77.411 bits (189), Expect = 1.442e-14 Identity = 49/177 (27.68%), Postives = 91/177 (51.41%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKEN 4903
+ +F+ W +DTD++G+IE EL NFL + + + L+ +D N DG++E+ E++++LP E FL+ FQ EF K ++ +D+D +G+I+ NEL + +L +KN + ++ + I + ++ L D GKL +++A +L +N
Sbjct: 99 SCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKA------NKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKFQGIKMCGK--EFNKAFELYDQDGNGYIDENELDALLKDLC---EKNKQDLDINNITTYKKNIMALSD---GGKLYRTDLALILCAGDN 261
BLAST of Calbindin-32 vs. Ensembl Human
Match: CALB2 (calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435]) HSP 1 Score: 177.178 bits (448), Expect = 3.170e-49 Identity = 95/213 (44.60%), Postives = 139/213 (65.26%), Query Frame = 2
Query: 4358 QLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQS-AFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRN 4993
L + TA+QFL++W HFD D NGYIE EL+NF + + G ++S+S F + F++ YD+N DG+IE+ EL+QILPT+E FL+ F++ + SS EFM+ W+++D DRSG+IEANEL+ F+++LLK K +E K+ +T +L++FD N DGKL LSEM+RLLP++EN+L K K++ E IF YD + N
Sbjct: 12 HLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARK----GSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLK---KANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK---FQGMKLTSEEFNAIFTFYDKEMN 212 HSP 2 Score: 80.4925 bits (197), Expect = 7.143e-16 Identity = 40/128 (31.25%), Postives = 76/128 (59.38%), Query Frame = 2
Query: 4661 SSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
++ +F++IWK FD D +G+IE EL+ F EL K + + + ++ +D+NSDGK++++E+A++LP +EN+L + + S E ++ YD DR+G+++ E++ L DL++
Sbjct: 17 TASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFL---LCFRQHVGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLK 141
BLAST of Calbindin-32 vs. Ensembl Human
Match: CALB2 (calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435]) HSP 1 Score: 177.178 bits (448), Expect = 3.170e-49 Identity = 95/213 (44.60%), Postives = 139/213 (65.26%), Query Frame = 2
Query: 4358 QLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQS-AFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRN 4993
L + TA+QFL++W HFD D NGYIE EL+NF + + G ++S+S F + F++ YD+N DG+IE+ EL+QILPT+E FL+ F++ + SS EFM+ W+++D DRSG+IEANEL+ F+++LLK K +E K+ +T +L++FD N DGKL LSEM+RLLP++EN+L K K++ E IF YD + N
Sbjct: 12 HLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARK----GSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLK---KANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK---FQGMKLTSEEFNAIFTFYDKEMN 212 HSP 2 Score: 80.4925 bits (197), Expect = 7.143e-16 Identity = 40/128 (31.25%), Postives = 76/128 (59.38%), Query Frame = 2
Query: 4661 SSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
++ +F++IWK FD D +G+IE EL+ F EL K + + + ++ +D+NSDGK++++E+A++LP +EN+L + + S E ++ YD DR+G+++ E++ L DL++
Sbjct: 17 TASQFLEIWKHFDADGNGYIEGKELENFFQELEKARKGSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFL---LCFRQHVGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLK 141
BLAST of Calbindin-32 vs. Ensembl Fly
Match: Cbp53E (gene:FBgn0004580 transcript:FBtr0340072) HSP 1 Score: 241.506 bits (615), Expect = 6.324e-71 Identity = 113/246 (45.93%), Postives = 176/246 (71.54%), Query Frame = 2
Query: 4319 NYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEE 5056
N++ +YR+ E+ +L K +A QF+DVW H+D D NGYIE ELD FL +F +S + I E ++ + + +K F+EAYD+N+DG+I+I EL+Q+LP +E FL+LF+ +NP+ SSVEFMKIW+ +D D SG+IEA+EL+ F+ +LLK +K V E K++ +T+ ML++FD N DG+LQLSEMA+LLP+KEN+L + + K++ ++E++F YD D +G ++ E++ L DL+EL ++
Sbjct: 19 NFMRQYRDPESR-ELKKLSANQFMDVWAHYDKDGNGYIEGTELDGFLREFVSSANATDISPEAVTDTMLEELKSCFMEAYDDNQDGKIDIRELAQLLPMEENFLLLFRFDNPLESSVEFMKIWREYDTDNSGYIEADELKNFLRDLLKEAKK-INDVSEDKLIEYTDTMLQVFDANKDGRLQLSEMAKLLPVKENFLCRQVFKGATKLTKEDIEKVFSLYDRDNSGTIENEELKGFLKDLLELVKK 262 HSP 2 Score: 80.1073 bits (196), Expect = 1.741e-15 Identity = 48/151 (31.79%), Postives = 87/151 (57.62%), Query Frame = 2
Query: 4643 RENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQK---NAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTE---EVRHSKLI 5077
RE S+ +FM +W +DKD +G+IE EL F+ E + + + E V ++ + + ++ +D N DGK+ + E+A+LLPM+EN+L +N + S +E +I++ YD D +G+++ E++ L DL++ + +V KLI
Sbjct: 30 RELKKLSANQFMDVWAHYDKDGNGYIEGTELDGFLREFVSSANATDISPEAVTDTMLEELKSCFMEAYDDNQDGKIDIRELAQLLPMEENFLLLFRFDNPLESS-VEFMKIWREYDTDNSGYIEADELKNFLRDLLKEAKKINDVSEDKLI 179
BLAST of Calbindin-32 vs. Ensembl Fly
Match: Cbp53E (gene:FBgn0004580 transcript:FBtr0087056) HSP 1 Score: 241.506 bits (615), Expect = 6.324e-71 Identity = 113/246 (45.93%), Postives = 176/246 (71.54%), Query Frame = 2
Query: 4319 NYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEE 5056
N++ +YR+ E+ +L K +A QF+DVW H+D D NGYIE ELD FL +F +S + I E ++ + + +K F+EAYD+N+DG+I+I EL+Q+LP +E FL+LF+ +NP+ SSVEFMKIW+ +D D SG+IEA+EL+ F+ +LLK +K V E K++ +T+ ML++FD N DG+LQLSEMA+LLP+KEN+L + + K++ ++E++F YD D +G ++ E++ L DL+EL ++
Sbjct: 19 NFMRQYRDPESR-ELKKLSANQFMDVWAHYDKDGNGYIEGTELDGFLREFVSSANATDISPEAVTDTMLEELKSCFMEAYDDNQDGKIDIRELAQLLPMEENFLLLFRFDNPLESSVEFMKIWREYDTDNSGYIEADELKNFLRDLLKEAKK-INDVSEDKLIEYTDTMLQVFDANKDGRLQLSEMAKLLPVKENFLCRQVFKGATKLTKEDIEKVFSLYDRDNSGTIENEELKGFLKDLLELVKK 262 HSP 2 Score: 80.1073 bits (196), Expect = 1.741e-15 Identity = 48/151 (31.79%), Postives = 87/151 (57.62%), Query Frame = 2
Query: 4643 RENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQK---NAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTE---EVRHSKLI 5077
RE S+ +FM +W +DKD +G+IE EL F+ E + + + E V ++ + + ++ +D N DGK+ + E+A+LLPM+EN+L +N + S +E +I++ YD D +G+++ E++ L DL++ + +V KLI
Sbjct: 30 RELKKLSANQFMDVWAHYDKDGNGYIEGTELDGFLREFVSSANATDISPEAVTDTMLEELKSCFMEAYDDNQDGKIDIRELAQLLPMEENFLLLFRFDNPLESS-VEFMKIWREYDTDNSGYIEADELKNFLRDLLKEAKKINDVSEDKLI 179
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: calb2a (calbindin 2a [Source:ZFIN;Acc:ZDB-GENE-040426-1677]) HSP 1 Score: 206.453 bits (524), Expect = 3.708e-59 Identity = 108/229 (47.16%), Postives = 151/229 (65.94%), Query Frame = 2
Query: 4358 QLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
L + TA+QFLD+W HFD D NGYIE ELDNF Q + + S +AF+ F++ +D+N+DGRIE+ EL+QILPT+E FL+ F++ + SS EFM W+++D DRSGFIEANEL+ F+++LLK ++ ++ K+ +T +LK+FD N DGKL LSEMARLLP++EN+L K ++S + IF YD D NG++DE E+ ALL DL E
Sbjct: 12 HLAELTASQFLDIWKHFDADGNGYIEGKELDNFFSQLEIARRGAGVDP---SNAAFKGRMTEFMQKFDKNKDGRIEMSELAQILPTEENFLLCFRQ--FVGSSTEFMAAWRKYDTDRSGFIEANELKGFLSDLLKKANRH---YDDQKLQEYTQTILKMFDLNGDGKLGLSEMARLLPVQENFLLK---FQGVRLSAEQFNAIFTYYDKDGNGYIDEQELDALLKDLYE 229
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: calb2b (calbindin 2b [Source:ZFIN;Acc:ZDB-GENE-040426-1668]) HSP 1 Score: 197.593 bits (501), Expect = 3.491e-56 Identity = 105/228 (46.05%), Postives = 154/228 (67.54%), Query Frame = 2
Query: 4358 QLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATS-VISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDL 5038
L + TA+QFLD+WN FD D NG IE EL+NF+ + + ++P S ++F+ + F++ +DEN DG+IE+ EL+QILPT+E FL+ F++ + SS EFM W+++D DRSG+IE+NEL+ F+++LLK ++ ++ K+ +T +LK+FD N DGKL LSEMARLLP++EN+L K +K+S E E IF YD D NG++DE E+ ALL DL
Sbjct: 12 HLAELTASQFLDIWNKFDADGNGCIEGKELENFIRELEIARKTADP------SNASFKDRMKEFMQKFDENGDGKIEMSELAQILPTEENFLLCFRQ--FVGSSAEFMTAWRKYDTDRSGYIESNELKGFLSDLLKKANRH---YDDKKLNEYTQTILKMFDLNGDGKLGLSEMARLLPVEENFLLK---FQNFKLSAEEFEAIFNYYDKDGNGYIDEHELDALLRDL 225 HSP 2 Score: 78.9518 bits (193), Expect = 3.317e-15 Identity = 46/140 (32.86%), Postives = 79/140 (56.43%), Query Frame = 2
Query: 4652 PIS------SSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIM---LKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
PIS ++ +F+ IW +FD D +G IE EL+ F+ EL + A K + F + M ++ FD N DGK+++SE+A++LP +EN+L + + S E ++ YD DR+G+++ E++ L DL++
Sbjct: 8 PISLHLAELTASQFLDIWNKFDADGNGCIEGKELENFIREL-----EIARKTADPSNASFKDRMKEFMQKFDENGDGKIEMSELAQILPTEENFL---LCFRQFVGSSAEFMTAWRKYDTDRSGYIESNELKGFLSDLLK 139
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: calb1 (calbindin 1 [Source:ZFIN;Acc:ZDB-GENE-030131-8107]) HSP 1 Score: 181.8 bits (460), Expect = 1.052e-50 Identity = 104/240 (43.33%), Postives = 154/240 (64.17%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEEVRHSKLIMINKI 5092
+A+QFLD+++H+D D NG+IE EL NF+ + + + G E+ + + F+E Y++N DG+I I EL QILPT+E FL+ F+++ + S EFM+ W+ +D D SG+IEA+EL+ F+ +LL Q+ + +E K+ +T LK+FD N DGKL LSEMARLLP EN+L K K++ E +IF+ YD DRNG+MDE E+ ALL DL E +++K++ NKI
Sbjct: 12 SASQFLDIFHHYDNDGNGFIEGKELQNFIKELQQA--RKQAGLELTDKM------KAFVEKYEQNADGKIGIIELVQILPTEENFLLFFRQQ--LKSCAEFMEAWRIYDADHSGYIEADELKNFLKDLL---QRAKKPYDEKKLEEYTLTTLKIFDSNKDGKLCLSEMARLLPDNENFLLKF---QGVKMARKEFNKIFELYDKDRNGYMDENELDALLKDLCE-----KNNKVLEANKI 230
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:573010]) HSP 1 Score: 179.874 bits (455), Expect = 5.306e-50 Identity = 90/227 (39.65%), Postives = 150/227 (66.08%), Query Frame = 2
Query: 4376 ATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELS-QILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISF-LELERIFKAYDVDRNGFMDETEMQALLVDLMELT 5050
A FL +W HFD D+NGYIE ELD+F + +P ++ ++ Q +K++F+ AYD DGR++I EL+ ILP +E FL++F+RE P+ +SVEFMKIW+++D D SG+I A EL+ F+ +L ++K K+ +K+ +T+ M+K+FD+N DG+L L+++AR+L ++EN+L + ++ + ++ + E+IF YDV R G ++ E+ + D+MEL
Sbjct: 10 AAGFLQIWQHFDADDNGYIEGKELDDFFRHMLKKL--QP--KDKITDERVQQIKKSFMSAYDATFDGRLQIEELANMILPQEENFLLIFRREAPLDNSVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQHKK---KIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQENFLLQFKMDASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELV 229 HSP 2 Score: 85.5001 bits (210), Expect = 2.249e-17 Identity = 53/179 (29.61%), Postives = 95/179 (53.07%), Query Frame = 2
Query: 4376 ATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVE----FMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKE 4900
+ +F+ +W +D D++GYI AAEL NFL ++ + + ++ +D+N+DGR+++ +L++IL E FL+ F+ + SS VE F KI+ +D R+G +E E+ FV ++++ + + + + F +L D N DGK+Q SE+A L +K
Sbjct: 103 SVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQ------HKKKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQENFLLQFKMD--ASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELVRPS---ISGGDLDKFRECLLTHCDMNKDGKIQKSELALCLGLKH 270
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:573010]) HSP 1 Score: 179.874 bits (455), Expect = 5.306e-50 Identity = 90/227 (39.65%), Postives = 150/227 (66.08%), Query Frame = 2
Query: 4376 ATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELS-QILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISF-LELERIFKAYDVDRNGFMDETEMQALLVDLMELT 5050
A FL +W HFD D+NGYIE ELD+F + +P ++ ++ Q +K++F+ AYD DGR++I EL+ ILP +E FL++F+RE P+ +SVEFMKIW+++D D SG+I A EL+ F+ +L ++K K+ +K+ +T+ M+K+FD+N DG+L L+++AR+L ++EN+L + ++ + ++ + E+IF YDV R G ++ E+ + D+MEL
Sbjct: 10 AAGFLQIWQHFDADDNGYIEGKELDDFFRHMLKKL--QP--KDKITDERVQQIKKSFMSAYDATFDGRLQIEELANMILPQEENFLLIFRREAPLDNSVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQHKK---KIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQENFLLQFKMDASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELV 229 HSP 2 Score: 85.5001 bits (210), Expect = 2.249e-17 Identity = 53/179 (29.61%), Postives = 95/179 (53.07%), Query Frame = 2
Query: 4376 ATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVE----FMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKE 4900
+ +F+ +W +D D++GYI AAEL NFL ++ + + ++ +D+N+DGR+++ +L++IL E FL+ F+ + SS VE F KI+ +D R+G +E E+ FV ++++ + + + + F +L D N DGK+Q SE+A L +K
Sbjct: 103 SVEFMKIWRKYDADSSGYISAAELKNFLKDLFLQ------HKKKIPPNKLDEYTDAMMKIFDKNKDGRLDLNDLARILALQENFLLQFKMD--ASSQVERKRDFEKIFAHYDVSRTGALEGPEVDGFVKDMMELVRPS---ISGGDLDKFRECLLTHCDMNKDGKIQKSELALCLGLKH 270
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: gata5 (GATA binding protein 5 [Source:Xenbase;Acc:XB-GENE-484906]) HSP 1 Score: 174.096 bits (440), Expect = 1.101e-48 Identity = 87/186 (46.77%), Postives = 126/186 (67.74%), Query Frame = 2
Query: 4358 QLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAK 4915
L + TA+QFLD+W H+D+D NGYIE EL+NF + + + S F + F++ YD+N DGRIE+ EL+QILPT+E FL+ F++ SS+EFM+ W+++D DRSG+IEANEL+ F+++LLK K +E K+ +T +L++FD N DGKL LSEM+RLLP++EN+L K
Sbjct: 11 HLAELTASQFLDIWKHYDSDGNGYIEGKELENFFRELEAARRGAGVDA---SNVNFGEKLKEFMQKYDKNADGRIEMAELAQILPTEENFLLCFRQH--AGSSIEFMEAWRKYDTDRSGYIEANELKGFLSDLLK---KANRPFDEKKLHEYTQTILRMFDLNGDGKLCLSEMSRLLPVQENFLLK 188 HSP 2 Score: 79.337 bits (194), Expect = 1.086e-15 Identity = 39/130 (30.00%), Postives = 79/130 (60.77%), Query Frame = 2
Query: 4661 SSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNI--MLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
++ +F+ IWK +D D +G+IE EL+ F EL + V+ S + + + ++ +D+N+DG+++++E+A++LP +EN+L + I F+E ++ YD DR+G+++ E++ L DL++
Sbjct: 16 TASQFLDIWKHYDSDGNGYIEGKELENFFREL--EAARRGAGVDASNVNFGEKLKEFMQKYDKNADGRIEMAELAQILPTEENFLLCFRQHAGSSIEFME---AWRKYDTDRSGYIEANELKGFLSDLLK 140 HSP 3 Score: 61.6178 bits (148), Expect = 1.061e-9 Identity = 37/121 (30.58%), Postives = 59/121 (48.76%), Query Frame = 2
Query: 4316 DNYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFM 4678
+N+L +R ++ +F++ W +DTD +GYIEA EL FL + P + L + QT L +D N DG++ + E+S++LP E FL+ FQ S + M
Sbjct: 95 ENFLLCFRQHAG-------SSIEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKA-NRPFDEKKLHEYT-----QTILRMFDLNGDGKLCLSEMSRLLPVQENFLLKFQGMKLSSDGLNLM 202
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: stim1 (stromal interaction molecule 1 [Source:Xenbase;Acc:XB-GENE-947969]) HSP 1 Score: 174.481 bits (441), Expect = 4.908e-48 Identity = 92/214 (42.99%), Postives = 141/214 (65.89%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETE 5014
+A+QFL++W H+DTD NG+IE EL NF+++ + + G ++ Q + F++ Y+ N DG+I I EL+QILPT+E FL+ F+++ + SS EFM+ W+R+D D SGFIE +EL+ F+ +LLK K EE+K+ +T+ ML +FD N+DGKL L+EM+ LLP++EN+L K K+ E ++F+ YD D NG++DE E
Sbjct: 17 SASQFLEIWRHYDTDGNGFIEGKELQNFILELQQA--RKKAGLDLSDQM------KAFVDQYENNTDGKIGIAELAQILPTEENFLLFFRQQ--LKSSEEFMQSWRRYDTDHSGFIETDELKSFLKDLLKKANK---PCEETKLEEYTHTMLLMFDTNNDGKLGLTEMSMLLPVQENFLLK---FQGVKMCGKEFNKVFELYDKDGNGYIDENE 214
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:780242]) HSP 1 Score: 174.096 bits (440), Expect = 9.364e-48 Identity = 90/231 (38.96%), Postives = 146/231 (63.20%), Query Frame = 2
Query: 4367 KFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELS-QILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAK-PMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTE 5053
K A +FL++W FD D+NGYIE ELD F + + +G ++ Q +K F+ AYD DGR++I EL+ ILP DE FL+LF+RE P+ +SVEFM+IW+++D+D SGFI A EL+ F+ +L ++K ++ K+ +T+ M+KLF++N DG+L L+++A++L ++EN+L + M ++ + E IF YDV + G ++ E+ + D+MEL +
Sbjct: 7 KLDAAEFLEIWQRFDADDNGYIEGKELDEFFCH-----LLKKLGANAITAEKVQRVKDRFMSAYDVTADGRLQIQELANMILPEDENFLLLFRRETPLDNSVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQHKK---QITGDKLDEYTDTMMKLFNKNKDGRLDLNDLAKILALQENFLLQFKMDASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVK 229 HSP 2 Score: 77.411 bits (189), Expect = 1.663e-14 Identity = 48/178 (26.97%), Postives = 94/178 (52.81%), Query Frame = 2
Query: 4376 ATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISS--SVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKEN 4903
+ +F+ +W +D D++G+I A EL NFL ++ ++ T ++ +++N+DGR+++ +L++IL E FL+ F+ + +F I+ +D ++G +E E+ FV ++++ + + V+ K F I+L D N DGK+Q SE+A L +K +
Sbjct: 102 SVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQ------HKKQITGDKLDEYTDTMMKLFNKNKDGRLDLNDLAKILALQENFLLQFKMDASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKPSISGVDLDK---FRQILLNHCDVNKDGKIQKSELALCLGLKPH 270
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: stim1 (stromal interaction molecule 1 [Source:Xenbase;Acc:XB-GENE-947969]) HSP 1 Score: 168.703 bits (426), Expect = 1.086e-45 Identity = 94/238 (39.50%), Postives = 147/238 (61.76%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNT------------------------YKISFLELERIFKAYDVDRNGFMDETE 5014
+A+QFL++W H+DTD NG+IE EL NF+++ + + G ++ Q MK F++ Y+ N DG+I I EL+QILPT+E FL+ F+++ + SS EFM+ W+R+D D SGFIE +EL+ F+ +LLK K + EE+K+ +T+ ML +FD N+DGKL L+EM+ LLP++EN+L K ++++ K+ E ++F+ YD D NG++DE E
Sbjct: 12 SASQFLEIWRHYDTDGNGFIEGKELQNFILELQQA--RKKAGLDLSDQ-----MK-AFVDQYENNTDGKIGIAELAQILPTEENFLLFFRQQ--LKSSEEFMQSWRRYDTDHSGFIETDELKSFLKDLLK---KANKPCEETKLEEYTHTMLLMFDTNNDGKLGLTEMSMLLPVQENFLLKFQVSSSVQPLQISVNFICVGINQMCFNPQGVKMCGKEFNKVFELYDKDGNGYIDENE 236 HSP 2 Score: 67.3958 bits (163), Expect = 4.947e-11 Identity = 55/202 (27.23%), Postives = 100/202 (49.50%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQREN---PISSSV----------------------EFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKEN 4903
++ +F+ W +DTD++G+IE EL +FL ++P ++ + T L +D N DG++ + E+S +LP E FL+ FQ + P+ SV EF K+++ +DKD +G+I+ NEL + + +L N+K+ ++ + + + ++ L D GKL +E+A +L EN
Sbjct: 98 SSEEFMQSWRRYDTDHSGFIETDELKSFLKDLLKKA-NKPC-----EETKLEEYTHTMLLMFDTNNDGKLGLTEMSMLLPVQENFLLKFQVSSSVQPLQISVNFICVGINQMCFNPQGVKMCGKEFNKVFELYDKDGNGYIDENELDDLLKDLCDKNKKD---LDINNLSTYKKSIMALSD---GGKLYRTELALVLCADEN 287
BLAST of Calbindin-32 vs. Ensembl Xenopus
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:780242]) HSP 1 Score: 166.392 bits (420), Expect = 3.432e-45 Identity = 87/230 (37.83%), Postives = 142/230 (61.74%), Query Frame = 2
Query: 4367 KFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELS-QILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTE 5053
K A +FL++W FD D+NGYIE ELD F + + +G ++ Q +K F+ AYD DGR++I EL+ ILP DE FL+LF+RE P+ +SVEFM+IW+++D+D SGFI A EL+ F+ +L ++K ++ K+ +T+ M+KLF++N DG+L L+++A+ + K ++L + ++ + E IF YDV + G ++ E+ + D+MEL +
Sbjct: 7 KLDAAEFLEIWQRFDADDNGYIEGKELDEFFCH-----LLKKLGANAITAEKVQRVKDRFMSAYDVTADGRLQIQELANMILPEDENFLLLFRRETPLDNSVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQHKK---QITGDKLDEYTDTMMKLFNKNKDGRLDLNDLAKYVFYK-DFLIGSLWASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVK 227 HSP 2 Score: 66.6254 bits (161), Expect = 6.376e-11 Identity = 42/176 (23.86%), Postives = 89/176 (50.57%), Query Frame = 2
Query: 4376 ATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKEN 4903
+ +F+ +W +D D++G+I A EL NFL ++ ++ T ++ +++N+DGR+++ +L++ + + + + +F I+ +D ++G +E E+ FV ++++ + + V+ K F I+L D N DGK+Q SE+A L +K +
Sbjct: 102 SVEFMRIWRKYDEDSSGFISAVELRNFLQDLFLQ------HKKQITGDKLDEYTDTMMKLFNKNKDGRLDLNDLAKYVFYKDFLIGSLWASSQDERKRDFETIFAHYDVSKTGALEGPEVDGFVKDMMELVKPSISGVDLDK---FRQILLNHCDVNKDGKIQKSELALCLGLKPH 268
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Calb2 (calbindin 2 [Source:MGI Symbol;Acc:MGI:101914]) HSP 1 Score: 196.438 bits (498), Expect = 4.497e-56 Identity = 105/230 (45.65%), Postives = 152/230 (66.09%), Query Frame = 2
Query: 4358 QLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSA-FQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
L + TA+QFL++W HFD D NGYIE EL+NF + + G ++S+S F + F++ YD+N DG+IE+ EL+QILPT+E FL+ F++ + SS EFM+ W+++D DRSG+IEANEL+ F+++LLK K +E K+ +T +L++FD N DGKL LSEM+RLLP++EN+L K K++ E IF YD D +G++DE E+ ALL DL E
Sbjct: 12 HLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARK----GSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLK---KANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK---FQGMKLTSEEFNAIFTFYDKDGSGYIDENELDALLKDLYE 229 HSP 2 Score: 88.1965 bits (217), Expect = 1.322e-18 Identity = 53/146 (36.30%), Postives = 81/146 (55.48%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFL---IQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESK 4801
++ +F++ W +DTD +GYIEA EL FL ++ A EP Q QT L +D N DG++ + E+S++LP E FL+ FQ +S EF I+ +DKD SG+I+ NEL + +L + N+K A + ES+
Sbjct: 108 SSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEP---------KLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQ--GMKLTSEEFNAIFTFYDKDGSGYIDENELDALLKDLYEKNKKEAREKTESR 242
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Calb2 (calbindin 2 [Source:MGI Symbol;Acc:MGI:101914]) HSP 1 Score: 196.438 bits (498), Expect = 1.322e-55 Identity = 105/230 (45.65%), Postives = 152/230 (66.09%), Query Frame = 2
Query: 4358 QLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSA-FQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
L + TA+QFL++W HFD D NGYIE EL+NF + + G ++S+S F + F++ YD+N DG+IE+ EL+QILPT+E FL+ F++ + SS EFM+ W+++D DRSG+IEANEL+ F+++LLK K +E K+ +T +L++FD N DGKL LSEM+RLLP++EN+L K K++ E IF YD D +G++DE E+ ALL DL E
Sbjct: 12 HLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARK----GSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLK---KANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK---FQGMKLTSEEFNAIFTFYDKDGSGYIDENELDALLKDLYE 229 HSP 2 Score: 85.1149 bits (209), Expect = 3.343e-17 Identity = 50/137 (36.50%), Postives = 76/137 (55.47%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFL---IQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQK 4774
++ +F++ W +DTD +GYIEA EL FL ++ A EP Q QT L +D N DG++ + E+S++LP E FL+ FQ +S EF I+ +DKD SG+I+ NEL + +L + N+K
Sbjct: 108 SSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEP---------KLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQGMK--LTSEEFNAIFTFYDKDGSGYIDENELDALLKDLYEKNKK 233
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Calb1 (calbindin 1 [Source:MGI Symbol;Acc:MGI:88248]) HSP 1 Score: 180.259 bits (456), Expect = 3.407e-50 Identity = 98/233 (42.06%), Postives = 150/233 (64.38%), Query Frame = 2
Query: 4346 ENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
E++ Q + TA+QF ++W HFD D +GY+E EL N + + + + G E+ + ++F++ Y + +DG+I I EL+ +LPT+E FL+LF R + S EFMK W+++D D SGFIE EL+ F+ +LL+ K V+++K+ +T++MLKLFD N+DGKL+L+EMARLLP++EN+L K K+ E + F+ YD D NG++DE E+ ALL DL E
Sbjct: 3 ESHLQSSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELLQA--RKKAGLELSPEM------KSFVDQYGQRDDGKIGIVELAHVLPTEENFLLLF-RCQQLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKANKT---VDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKF---QGIKMCGKEFNKAFELYDQDGNGYIDENELDALLKDLCE 220 HSP 2 Score: 77.7962 bits (190), Expect = 7.545e-15 Identity = 49/177 (27.68%), Postives = 92/177 (51.98%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKEN 4903
+ +F+ W +DTD++G+IE EL NFL + + + L+ +D N DG++E+ E++++LP E FL+ FQ EF K ++ +D+D +G+I+ NEL + +L +KN ++++ + I + ++ L D GKL +++A +L +N
Sbjct: 99 SCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKA------NKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKFQGIKMCGK--EFNKAFELYDQDGNGYIDENELDALLKDLC---EKNKQELDINNITTYKKNIMALSD---GGKLYRTDLALILSAGDN 261
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Scgn (secretagogin, EF-hand calcium binding protein [Source:MGI Symbol;Acc:MGI:2384873]) HSP 1 Score: 147.132 bits (370), Expect = 1.570e-38 Identity = 80/231 (34.63%), Postives = 139/231 (60.17%), Query Frame = 2
Query: 4367 KFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGEL-SQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAK-PMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTE 5053
+ A F +W FD + GYI ELD F +E + L + Q +K+ + +++ +++GRI + EL S L DE FL+ F+ E P+ +SVEFM+IW+++D D SGFI A EL F+ +L +++KN + E+++ +T+ M+K+FD+N DG+L L+++AR+L ++EN+L + M ++ + + E+IF YDV + G ++ E+ + D+MEL +
Sbjct: 11 RLDAACFWQIWQRFDKEEKGYIRETELDAFFDHLLAKSGTE----DTLMEENVQKVKEQLMTSHNVSKEGRILMKELASMFLSEDENFLLFFRLETPLDNSVEFMQIWRKYDADSSGFISAAELCNFLRDLFLHHKKN---ISEAELEEYTSTMMKIFDKNKDGRLDLNDLARILALQENFLLQFKMDASSTEERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVQ 234 HSP 2 Score: 92.0485 bits (227), Expect = 1.223e-19 Identity = 57/181 (31.49%), Postives = 103/181 (56.91%), Query Frame = 2
Query: 4376 ATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVE-----FMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKEN 4903
+ +F+ +W +D D++G+I AAEL NFL ++ +S++ + T ++ +D+N+DGR+++ +L++IL E FL+ F+ + +SS E F KI+ +D ++G +E E+ FV ++++ Q + V+ K F I+L+ D N DGK+Q SE+A L +K N
Sbjct: 107 SVEFMQIWRKYDADSSGFISAAELCNFLRDLFLH------HKKNISEAELEEYTSTMMKIFDKNKDGRLDLNDLARILALQENFLLQFKMD---ASSTEERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVQPSISGVDLDK---FREILLRHCDVNKDGKIQKSELALCLGLKIN 275
BLAST of Calbindin-32 vs. Ensembl Mouse
Match: Scgn (secretagogin, EF-hand calcium binding protein [Source:MGI Symbol;Acc:MGI:2384873]) HSP 1 Score: 66.6254 bits (161), Expect = 1.988e-12 Identity = 42/124 (33.87%), Postives = 74/124 (59.68%), Query Frame = 2
Query: 4547 LEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVE-----FMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKEN 4903
++ +D+N+DGR+++ +L++IL E FL+ F+ + +SS E F KI+ +D ++G +E E+ FV ++++ Q + V+ K F I+L+ D N DGK+Q SE+A L +K N
Sbjct: 1 MKIFDKNKDGRLDLNDLARILALQENFLLQFKMD---ASSTEERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVQPSISGVDLDK---FREILLRHCDVNKDGKIQKSELALCLGLKIN 118 HSP 2 Score: 55.0694 bits (131), Expect = 2.755e-8 Identity = 27/77 (35.06%), Postives = 51/77 (66.23%), Query Frame = 2
Query: 4826 LKLFDRNSDGKLQLSEMARLLPMKENYLAK-PMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTE 5053
+K+FD+N DG+L L+++AR+L ++EN+L + M ++ + + E+IF YDV + G ++ E+ + D+MEL +
Sbjct: 1 MKIFDKNKDGRLDLNDLARILALQENFLLQFKMDASSTEERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVQ 77
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|P41044|CAB32_DROME (Calbindin-32 OS=Drosophila melanogaster OX=7227 GN=Cbp53E PE=2 SV=1) HSP 1 Score: 241.506 bits (615), Expect = 6.339e-70 Identity = 113/246 (45.93%), Postives = 176/246 (71.54%), Query Frame = 2
Query: 4319 NYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEE 5056
N++ +YR+ E+ +L K +A QF+DVW H+D D NGYIE ELD FL +F +S + I E ++ + + +K F+EAYD+N+DG+I+I EL+Q+LP +E FL+LF+ +NP+ SSVEFMKIW+ +D D SG+IEA+EL+ F+ +LLK +K V E K++ +T+ ML++FD N DG+LQLSEMA+LLP+KEN+L + + K++ ++E++F YD D +G ++ E++ L DL+EL ++
Sbjct: 19 NFMRQYRDPESR-ELKKLSANQFMDVWAHYDKDGNGYIEGTELDGFLREFVSSANATDISPEAVTDTMLEELKSCFMEAYDDNQDGKIDIRELAQLLPMEENFLLLFRFDNPLESSVEFMKIWREYDTDNSGYIEADELKNFLRDLLKEAKK-INDVSEDKLIEYTDTMLQVFDANKDGRLQLSEMAKLLPVKENFLCRQVFKGATKLTKEDIEKVFSLYDRDNSGTIENEELKGFLKDLLELVKK 262 HSP 2 Score: 80.1073 bits (196), Expect = 1.745e-14 Identity = 48/151 (31.79%), Postives = 87/151 (57.62%), Query Frame = 2
Query: 4643 RENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQK---NAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTE---EVRHSKLI 5077
RE S+ +FM +W +DKD +G+IE EL F+ E + + + E V ++ + + ++ +D N DGK+ + E+A+LLPM+EN+L +N + S +E +I++ YD D +G+++ E++ L DL++ + +V KLI
Sbjct: 30 RELKKLSANQFMDVWAHYDKDGNGYIEGTELDGFLREFVSSANATDISPEAVTDTMLEELKSCFMEAYDDNQDGKIDIRELAQLLPMEENFLLLFRFDNPLESS-VEFMKIWREYDTDNSGYIEADELKNFLRDLLKEAKKINDVSEDKLI 179
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|P07090|CALB2_CHICK (Calretinin OS=Gallus gallus OX=9031 GN=CALB2 PE=2 SV=3) HSP 1 Score: 201.445 bits (511), Expect = 1.382e-56 Identity = 110/234 (47.01%), Postives = 154/234 (65.81%), Query Frame = 2
Query: 4343 RENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
R + L + +A+QFLDVW HFD D NGYIE EL+NF + ++ + G + S MK+ F+ YD+N DG+IE+ EL+QILPT+E FL+ F++ + SS EFM+ W+R+D DRSG+IEANEL+ F+++LLK K +E+K+ +T +L++FD N DGKL LSEM+RLLP++EN+L K K+S E IF YD D +GF+DE E+ ALL DL E
Sbjct: 5 RAPHLHLAELSASQFLDVWRHFDADGNGYIEGKELENFFQELESA--RKGTGVDSKRDSLGDKMKE-FMHKYDKNADGKIEMAELAQILPTEENFLLCFRQH--VGSSSEFMEAWRRYDTDRSGYIEANELKGFLSDLLK---KANRPYDEAKLQEYTQTILRMFDMNGDGKLGLSEMSRLLPVQENFLLK---FQGMKLSSEEFNAIFAFYDKDGSGFIDEHELDALLKDLYE 227 HSP 2 Score: 88.9669 bits (219), Expect = 1.109e-17 Identity = 58/172 (33.72%), Postives = 90/172 (52.33%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLL 4888
++++F++ W +DTD +GYIEA EL FL + P ++ Q QT L +D N DG++ + E+S++LP E FL+ FQ SS EF I+ +DKD SGFI+ +EL + +L + N+K E I TN + + + GKL E+ +L
Sbjct: 106 SSSEFMEAWRRYDTDRSGYIEANELKGFLSDLLKKA-NRPY-----DEAKLQEYTQTILRMFDMNGDGKLGLSEMSRLLPVQENFLLKFQ--GMKLSSEEFNAIFAFYDKDGSGFIDEHELDALLKDLYEKNKK------EMSIQQLTNYRRSIMNLSDGGKLYRKELEVVL 263
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|P22676|CALB2_HUMAN (Calretinin OS=Homo sapiens OX=9606 GN=CALB2 PE=2 SV=2) HSP 1 Score: 199.134 bits (505), Expect = 9.992e-56 Identity = 106/230 (46.09%), Postives = 153/230 (66.52%), Query Frame = 2
Query: 4358 QLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSA-FQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
L + TA+QFL++W HFD D NGYIE EL+NF + + G ++S+S F + F++ YD+N DG+IE+ EL+QILPT+E FL+ F++ + SS EFM+ W+++D DRSG+IEANEL+ F+++LLK K +E K+ +T +L++FD N DGKL LSEM+RLLP++EN+L K K++ E IF YD DR+G++DE E+ ALL DL E
Sbjct: 12 HLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARK----GSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLK---KANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK---FQGMKLTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYE 229 HSP 2 Score: 86.2705 bits (212), Expect = 7.857e-17 Identity = 50/137 (36.50%), Postives = 77/137 (56.20%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFL---IQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQK 4774
++ +F++ W +DTD +GYIEA EL FL ++ A EP Q QT L +D N DG++ + E+S++LP E FL+ FQ +S EF I+ +DKDRSG+I+ +EL + +L + N+K
Sbjct: 108 SSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEP---------KLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQGMK--LTSEEFNAIFTFYDKDRSGYIDEHELDALLKDLYEKNKK 233
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|P47728|CALB2_RAT (Calretinin OS=Rattus norvegicus OX=10116 GN=Calb2 PE=1 SV=1) HSP 1 Score: 196.438 bits (498), Expect = 8.562e-55 Identity = 105/230 (45.65%), Postives = 152/230 (66.09%), Query Frame = 2
Query: 4358 QLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSA-FQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
L + TA+QFL++W HFD D NGYIE EL+NF + + G ++S+S F + F++ YD+N DG+IE+ EL+QILPT+E FL+ F++ + SS EFM+ W+++D DRSG+IEANEL+ F+++LLK K +E K+ +T +L++FD N DGKL LSEM+RLLP++EN+L K K++ E IF YD D +G++DE E+ ALL DL E
Sbjct: 12 HLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARK----GSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLK---KANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK---FQGMKLTSEEFNAIFTFYDKDGSGYIDENELDALLKDLYE 229 HSP 2 Score: 85.1149 bits (209), Expect = 2.190e-16 Identity = 50/137 (36.50%), Postives = 76/137 (55.47%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFL---IQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQK 4774
++ +F++ W +DTD +GYIEA EL FL ++ A EP Q QT L +D N DG++ + E+S++LP E FL+ FQ +S EF I+ +DKD SG+I+ NEL + +L + N+K
Sbjct: 108 SSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEP---------KLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQGMK--LTSEEFNAIFTFYDKDGSGYIDENELDALLKDLYEKNKK 233
BLAST of Calbindin-32 vs. UniProt/SwissProt
Match: sp|Q08331|CALB2_MOUSE (Calretinin OS=Mus musculus OX=10090 GN=Calb2 PE=1 SV=3) HSP 1 Score: 196.438 bits (498), Expect = 9.248e-55 Identity = 105/230 (45.65%), Postives = 152/230 (66.09%), Query Frame = 2
Query: 4358 QLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSA-FQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
L + TA+QFL++W HFD D NGYIE EL+NF + + G ++S+S F + F++ YD+N DG+IE+ EL+QILPT+E FL+ F++ + SS EFM+ W+++D DRSG+IEANEL+ F+++LLK K +E K+ +T +L++FD N DGKL LSEM+RLLP++EN+L K K++ E IF YD D +G++DE E+ ALL DL E
Sbjct: 12 HLAELTASQFLEIWKHFDADGNGYIEGKELENFFQELEKARK----GSGMMSKSDNFGEKMKEFMQKYDKNSDGKIEMAELAQILPTEENFLLCFRQH--VGSSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLK---KANRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLK---FQGMKLTSEEFNAIFTFYDKDGSGYIDENELDALLKDLYE 229 HSP 2 Score: 85.1149 bits (209), Expect = 2.338e-16 Identity = 50/137 (36.50%), Postives = 76/137 (55.47%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFL---IQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQK 4774
++ +F++ W +DTD +GYIEA EL FL ++ A EP Q QT L +D N DG++ + E+S++LP E FL+ FQ +S EF I+ +DKD SG+I+ NEL + +L + N+K
Sbjct: 108 SSAEFMEAWRKYDTDRSGYIEANELKGFLSDLLKKANRPYDEP---------KLQEYTQTILRMFDLNGDGKLGLSEMSRLLPVQENFLLKFQGMK--LTSEEFNAIFTFYDKDGSGYIDENELDALLKDLYEKNKK 233
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A267FNU4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030011g3 PE=4 SV=1) HSP 1 Score: 290.426 bits (742), Expect = 1.244e-84 Identity = 138/246 (56.10%), Postives = 190/246 (77.24%), Query Frame = 2
Query: 4319 NYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEE 5056
N+L+++R+ + +L FTATQF +VW+H+D D NG+IE AELD FL +F +SV+SE G EV+S++A MK+ F+EA+DEN DG+IEIGELSQILPT+E FL+LF+R NP+ SSVEFM+IWK+FDKD SGFIEA+EL++F+ +L + K V E K++ +T+ ML+LFD+N DGKLQLSEMARLLP+KENYL +P+ N KIS +++R+F YD D+NG ++ E+Q L DL+EL +E
Sbjct: 8 NFLSQFRD-QGTKKLKNFTATQFAEVWSHYDQDGNGFIEGAELDEFLHEFISSVVSEDAGNEVVSETAMAQMKKDFMEAFDENLDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWKQFDKDNSGFIEADELKDFLKSILDSADK---PVTEDKLIEYTDTMLQLFDQNRDGKLQLSEMARLLPVKENYLCRPIFKNASKISSSDIDRVFSLYDRDKNGTIENEELQGFLKDLLELAQE 249
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A267GV22 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030011g1 PE=4 SV=1) HSP 1 Score: 290.426 bits (742), Expect = 1.268e-84 Identity = 138/246 (56.10%), Postives = 190/246 (77.24%), Query Frame = 2
Query: 4319 NYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEE 5056
N+L+++R+ + +L FTATQF +VW+H+D D NG+IE AELD FL +F +SV+SE G EV+S++A MK+ F+EA+DEN DG+IEIGELSQILPT+E FL+LF+R NP+ SSVEFM+IWK+FDKD SGFIEA+EL++F+ +L + K V E K++ +T+ ML+LFD+N DGKLQLSEMARLLP+KENYL +P+ N KIS +++R+F YD D+NG ++ E+Q L DL+EL +E
Sbjct: 8 NFLSQFRD-QGTKKLKNFTATQFAEVWSHYDQDGNGFIEGAELDEFLHEFISSVVSEDAGNEVVSETAMAQMKKDFMEAFDENLDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWKQFDKDNSGFIEADELKDFLKSILDSADK---PVTEDKLIEYTDTMLQLFDQNRDGKLQLSEMARLLPVKENYLCRPIFKNASKISSSDIDRVFSLYDRDKNGTIENEELQGFLKDLLELAQE 249
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A267GD68 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030011g2 PE=4 SV=1) HSP 1 Score: 290.041 bits (741), Expect = 1.509e-84 Identity = 138/246 (56.10%), Postives = 190/246 (77.24%), Query Frame = 2
Query: 4319 NYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEE 5056
N+L+++R+ + +L FTATQF +VW+H+D D NG+IE AELD FL +F +SV+SE G EV+S++A MK+ F+EA+DEN DG+IEIGELSQILPT+E FL+LF+R NP+ SSVEFM+IWK+FDKD SGFIEA+EL++F+ +L + K V E K++ +T+ ML+LFD+N DGKLQLSEMARLLP+KENYL +P+ N KIS +++R+F YD D+NG ++ E+Q L DL+EL +E
Sbjct: 8 NFLSQFRD-QGTKKLKNFTATQFAEVWSHYDQDGNGFIEGAELDEFLHEFISSVVSEDAGNEVVSETAMAQMKRDFMEAFDENLDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWKQFDKDNSGFIEADELKDFLKSILDSADK---PVTEDKLIEYTDTMLQLFDQNRDGKLQLSEMARLLPVKENYLCRPIFKNASKISSSDIDRVFSLYDRDKNGTIENEELQGFLKDLLELAQE 249
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A1I8GV54 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003004g1 PE=4 SV=1) HSP 1 Score: 281.952 bits (720), Expect = 1.011e-81 Identity = 132/246 (53.66%), Postives = 190/246 (77.24%), Query Frame = 2
Query: 4319 NYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEE 5056
N+L ++R+ +N +L FTATQF +VW+H+D D NG+IE ELD FL +F +SV++E IG EV+S++A AMK+ F++A+DEN DG+IEIGELSQILPT+E FL+LF+R NP+ SSVEFM+IW++FDKD SG+IEA+EL++F+ +L + V E K++ +T+ ML+LFD+N DGKLQLSEMA+LLP+KENYL +P+ N +I+ +++R+F YD D+NG ++ E+Q L DL+EL +E
Sbjct: 15 NFLAQFRD-QNTRRLKNFTATQFAEVWSHYDKDGNGFIEGQELDEFLREFISSVVTEDIGPEVISETALAAMKRDFMDAFDENYDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWRQFDKDHSGYIEADELRDFLKHILDCADRC---VSEDKLLEYTSTMLQLFDQNRDGKLQLSEMAKLLPVKENYLCRPIFKNANRINSNDIDRVFNLYDRDKNGCIENEELQGFLKDLLELAQE 256 HSP 2 Score: 95.9005 bits (237), Expect = 2.717e-17 Identity = 64/207 (30.92%), Postives = 120/207 (57.97%), Query Frame = 2
Query: 4316 DNYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFL---IQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLV--LFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPM 4921
+N+L +R NN ++ ++ +F+ +W FD D++GYIEA EL +FL + A +SE E S T L+ +D+N DG++++ E++++LP E +L +F+ N I+S+ + +++ +D+D++G IE ELQ F+ +LL+ Q E + +++F ++L +D N DGK+ E+ +L ++++ LA M
Sbjct: 114 ENFLLLFRR---NNPVD--SSVEFMRIWRQFDKDHSGYIEADELRDFLKHILDCADRCVSEDKLLEYTS---------TMLQLFDQNRDGKLQLSEMAKLLPVKENYLCRPIFKNANRINSN-DIDRVFNLYDRDKNGCIENEELQGFLKDLLELAQ---EDYDAQDMLFFKKVILDQWDVNHDGKISKDELKMIL-LQQSRLASQM 301
BLAST of Calbindin-32 vs. TrEMBL
Match: A0A267G6Z8 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig033745g2 PE=4 SV=1) HSP 1 Score: 281.952 bits (720), Expect = 1.041e-81 Identity = 132/246 (53.66%), Postives = 190/246 (77.24%), Query Frame = 2
Query: 4319 NYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEE 5056
N+L ++R+ +N +L FTATQF +VW+H+D D NG+IE ELD FL +F +SV++E IG EV+S++A AMK+ F++A+DEN DG+IEIGELSQILPT+E FL+LF+R NP+ SSVEFM+IW++FDKD SG+IEA+EL++F+ +L + V E K++ +T+ ML+LFD+N DGKLQLSEMA+LLP+KENYL +P+ N +I+ +++R+F YD D+NG ++ E+Q L DL+EL +E
Sbjct: 15 NFLAQFRD-QNTRRLKNFTATQFAEVWSHYDKDGNGFIEGQELDEFLREFISSVVTEDIGPEVISETALAAMKRDFMDAFDENYDGKIEIGELSQILPTEENFLLLFRRNNPVDSSVEFMRIWRQFDKDHSGYIEADELRDFLKHILDCADRC---VSEDKLLEYTSTMLQLFDQNRDGKLQLSEMAKLLPVKENYLCRPIFKNANRINSNDIDRVFNLYDRDKNGCIENEELQGFLKDLLELAQE 256 HSP 2 Score: 95.9005 bits (237), Expect = 2.667e-17 Identity = 64/207 (30.92%), Postives = 120/207 (57.97%), Query Frame = 2
Query: 4316 DNYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFL---IQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLV--LFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPM 4921
+N+L +R NN ++ ++ +F+ +W FD D++GYIEA EL +FL + A +SE E S T L+ +D+N DG++++ E++++LP E +L +F+ N I+S+ + +++ +D+D++G IE ELQ F+ +LL+ Q E + +++F ++L +D N DGK+ E+ +L ++++ LA M
Sbjct: 114 ENFLLLFRR---NNPVD--SSVEFMRIWRQFDKDHSGYIEADELRDFLKHILDCADRCVSEDKLLEYTS---------TMLQLFDQNRDGKLQLSEMAKLLPVKENYLCRPIFKNANRINSN-DIDRVFNLYDRDKNGCIENEELQGFLKDLLELAQ---EDYDAQDMLFFKKVILDQWDVNHDGKISKDELKMIL-LQQSRLASQM 301
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: calb2a (calretinin [Source:NCBI gene;Acc:103033342]) HSP 1 Score: 200.675 bits (509), Expect = 2.696e-57 Identity = 109/229 (47.60%), Postives = 153/229 (66.81%), Query Frame = 2
Query: 4358 QLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
L + TA+QFLD+W HFDTD NGYIE EL+NF + V G + + S + MK F++ +D+N+DGRIE+ EL+QILPT+E FL+ F++ + SS EFM W+R+D DRSG+IEANEL+ F+++LLK ++ ++ K+ +T +LK+FD N DGKL LSEMARLLP++EN+L K K++ + IF YD D NG++DE E+ ALL DL E
Sbjct: 12 HLAELTASQFLDIWKHFDTDGNGYIEGKELENFFREL--EVARRGAGVDPTNVSFREKMKD-FMQKFDKNKDGRIEMSELAQILPTEENFLLCFRQ--FVGSSSEFMAAWRRYDTDRSGYIEANELKGFLSDLLKKANRH---YDDKKLQEYTQTILKMFDLNGDGKLGLSEMARLLPVQENFLLK---FQGIKLTAEQFNAIFTYYDKDGNGYIDEQELDALLRDLYE 229 HSP 2 Score: 78.5666 bits (192), Expect = 3.537e-15 Identity = 57/178 (32.02%), Postives = 94/178 (52.81%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVE-FMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLL---PM 4894
++++F+ W +DTD +GYIEA EL FL S + + R Q QT L+ +D N DG++ + E++++LP E FL+ FQ I + E F I+ +DKD +G+I+ EL + +L +KN ++V+ + + ++ L D GKL SE+ +L PM
Sbjct: 108 SSSEFMAAWRRYDTDRSGYIEANELKGFL-----SDLLKKANRHY-DDKKLQEYTQTILKMFDLNGDGKLGLSEMARLLPVQENFLLKFQ---GIKLTAEQFNAIFTYYDKDGNGYIDEQELDALLRDLY---EKNKKEVDTKDLTGYKKSIMALSD---GGKLYRSELEIVLCREPM 270
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: calb2b (calretinin-like [Source:NCBI gene;Acc:103034108]) HSP 1 Score: 186.422 bits (472), Expect = 2.863e-52 Identity = 105/230 (45.65%), Postives = 156/230 (67.83%), Query Frame = 2
Query: 4358 QLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATS---VISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDL 5038
L + TA+QF+DVW HFD+D NG+IE EL++FL + ++ V +EP+ ++ + F++ +D+N DG+IE+ EL+QILPT+E FL+ F++ + SS EFM W+++D DRSGFIEANEL+ F+++LL K + ++ K+ +T+ +LK+FD N DGKL LSEMARLLP++EN+L K ++K+S E IF YD D NG++DE E+ ALL DL
Sbjct: 9 HLAELTASQFMDVWRHFDSDGNGFIEGQELEDFLEELESARKGVNAEPV------NASLRMKISDFMQKFDQNSDGKIEMLELAQILPTEENFLLCFRQ--FVGSSAEFMTAWRKYDTDRSGFIEANELKGFLSDLL---TKANRQYDDKKLNEYTHTILKMFDLNGDGKLGLSEMARLLPVQENFLLK---FQSFKLSSDEFSAIFMYYDKDGNGYIDEHELDALLKDL 224 HSP 2 Score: 75.8702 bits (185), Expect = 2.633e-14 Identity = 48/128 (37.50%), Postives = 80/128 (62.50%), Query Frame = 2
Query: 4661 SSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQK-NAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLM 5041
++ +FM +W+ FD D +GFIE EL++F+ EL + NAE V S + ++ M K FD+NSDGK+++ E+A++LP +EN+L + + S E ++ YD DR+GF++ E++ L DL+
Sbjct: 14 TASQFMDVWRHFDSDGNGFIEGQELEDFLEELESARKGVNAEPVNASLRMKISDFMQK-FDQNSDGKIEMLELAQILPTEENFL---LCFRQFVGSSAEFMTAWRKYDTDRSGFIEANELKGFLSDLL 137 HSP 3 Score: 72.7886 bits (177), Expect = 3.145e-13 Identity = 42/132 (31.82%), Postives = 72/132 (54.55%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNN 4768
++ +F+ W +DTD +G+IEA EL FL T + +++ T L+ +D N DG++ + E++++LP E FL+ FQ SS EF I+ +DKD +G+I+ +EL + +L K +
Sbjct: 105 SSAEFMTAWRKYDTDRSGFIEANELKGFLSDLLTKANRQYDDKKL------NEYTHTILKMFDLNGDGKLGLSEMARLLPVQENFLLKFQSFKL--SSDEFSAIFMYYDKDGNGYIDEHELDALLKDLYKKH 228
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: calb1 (calbindin 1 [Source:NCBI gene;Acc:103033937]) HSP 1 Score: 184.111 bits (466), Expect = 1.060e-51 Identity = 105/240 (43.75%), Postives = 155/240 (64.58%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEEVRHSKLIMINKI 5092
+A+QFLD+++H+D D NGYIE EL NF+ + + + G E+ Q + F+E Y++N DG+I+I EL QILPT+E FL+ F+++ + S EFM+ W+ +D D SG+IE++EL+ F+ +LL Q+ + +E K+ +T LK+FD N+DGKL LSEMARLLP EN+L K K++ E +IF+ YD DRNG+MDE E+ ALL DL E ++ K++ I KI
Sbjct: 12 SASQFLDIFHHYDNDGNGYIEGKELQNFIKELQQA--RKQAGLELTDQM------RAFVEEYEKNADGKIDIVELVQILPTEENFLLFFRQQ--LKSCTEFMEAWRTYDADHSGYIESDELKNFLRDLL---QRAKKPFDEKKLEEYTLTTLKIFDSNNDGKLCLSEMARLLPDDENFLLKF---EGVKMARKEFNKIFELYDKDRNGYMDENELDALLKDLCE-----KNKKVLEIGKI 230
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: calb2b (calretinin-like [Source:NCBI gene;Acc:103034108]) HSP 1 Score: 182.185 bits (461), Expect = 7.654e-51 Identity = 106/228 (46.49%), Postives = 155/228 (67.98%), Query Frame = 2
Query: 4358 QLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQT-FLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDL 5038
L + TA+QF+DVW HFD+D NG+IE EL++FL + ++ + V +A MK + F++ +D+N DG+IE+ EL+QILPT+E FL+ F++ + SS EFM W+++D DRSGFIEANEL+ F+++LL K + ++ K+ +T+ +LK+FD N DGKL LSEMARLLP++EN+L K ++K+S E IF YD D NG++DE E+ ALL DL
Sbjct: 15 HLAELTASQFMDVWRHFDSDGNGFIEGQELEDFLEELESAR------KGVNPVNASLRMKISDFMQKFDQNSDGKIEMLELAQILPTEENFLLCFRQF--VGSSAEFMTAWRKYDTDRSGFIEANELKGFLSDLL---TKANRQYDDKKLNEYTHTILKMFDLNGDGKLGLSEMARLLPVQENFLLK---FQSFKLSSDEFSAIFMYYDKDGNGYIDEHELDALLKDL 228 HSP 2 Score: 73.559 bits (179), Expect = 2.078e-13 Identity = 44/127 (34.65%), Postives = 76/127 (59.84%), Query Frame = 2
Query: 4661 SSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLM 5041
++ +FM +W+ FD D +GFIE + E E L++ +K V S + ++ M K FD+NSDGK+++ E+A++LP +EN+L + + S E ++ YD DR+GF++ E++ L DL+
Sbjct: 20 TASQFMDVWRHFDSDGNGFIEG-QELEDFLEELESARKGVNPVNASLRMKISDFMQK-FDQNSDGKIEMLELAQILPTEENFL---LCFRQFVGSSAEFMTAWRKYDTDRSGFIEANELKGFLSDLL 141 HSP 3 Score: 72.7886 bits (177), Expect = 3.158e-13 Identity = 42/132 (31.82%), Postives = 72/132 (54.55%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNN 4768
++ +F+ W +DTD +G+IEA EL FL T + +++ T L+ +D N DG++ + E++++LP E FL+ FQ SS EF I+ +DKD +G+I+ +EL + +L K +
Sbjct: 109 SSAEFMTAWRKYDTDRSGFIEANELKGFLSDLLTKANRQYDDKKL------NEYTHTILKMFDLNGDGKLGLSEMARLLPVQENFLLKFQSFKL--SSDEFSAIFMYYDKDGNGYIDEHELDALLKDLYKKH 232
BLAST of Calbindin-32 vs. Ensembl Cavefish
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:103030695]) HSP 1 Score: 180.259 bits (456), Expect = 3.781e-50 Identity = 96/232 (41.38%), Postives = 148/232 (63.79%), Query Frame = 2
Query: 4361 LNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELS-QILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAK-PMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELT 5050
L+ A FL +W HFD D+NGYIE ELD F T + P G V ++ Q +K F+ AYD DGR++I EL+ ILP DE FL++F+RE P+ +SVEFMKIW+++D D SG+I A EL+ F+ +L ++++K ++ SK+ +T+ M+ +FD+N DG+L L+++AR+L ++EN+L + M NT + E+IF YDV + G ++ E+ + D+MEL
Sbjct: 5 LDNLDAAGFLQIWQHFDADDNGYIEGKELDAFFRHMMTKL--RP-GDNV-TEEKVQQLKARFMSAYDVTADGRLQIQELANMILPEDENFLLVFRREAPLDNSVEFMKIWRKYDADSSGYISAVELKSFLQDLFQHHKK---ELSSSKLEEYTDTMMNIFDKNKDGRLDLNDLARILALQENFLLQFKMDANTLLERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELV 229 HSP 2 Score: 87.8113 bits (216), Expect = 3.074e-18 Identity = 52/176 (29.55%), Postives = 97/176 (55.11%), Query Frame = 2
Query: 4376 ATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRE--NPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMK 4897
+ +F+ +W +D D++GYI A EL +FL E LS S + T + +D+N+DGR+++ +L++IL E FL+ F+ + + +F KI+ +D ++G +E E+ FV ++++ + + + S++ F ++L+ D N DGK+Q +E+A L MK
Sbjct: 103 SVEFMKIWRKYDADSSGYISAVELKSFLQDLFQHHKKE------LSSSKLEEYTDTMMNIFDKNKDGRLDLNDLARILALQENFLLQFKMDANTLLERKRDFEKIFAHYDVSKTGALEGPEVDGFVKDMMELVRPS---ISGSELDQFRKVLLRHCDINKDGKIQKNELALCLGMK 269
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Match: scgn (secretagogin, EF-hand calcium binding protein [Source:NCBI gene;Acc:573010]) HSP 1 Score: 171.785 bits (434), Expect = 6.505e-48 Identity = 87/232 (37.50%), Postives = 146/232 (62.93%), Query Frame = 2
Query: 4361 LNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELS-QILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAK-PMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELT 5050
+ + L+VW FD D+NGYIE ELD F ++ + +E +S + +++ + YDE+ DGR+ + E++ +L DE FL++F+R + + +SVEF+KIW ++D D SGFI A+EL+ F+ +LL ++ + +VEE K+ +T+ M+KLFD+N DG+L L++MAR+L +KENYL + + + + + + E+IF YDV R G ++ E+ + D+MEL
Sbjct: 6 IGHLDSQGLLEVWQKFDADDNGYIEGKELDEFF----KHLLKQLAAKECVSDEKVETVRRNLMSIYDESADGRLHLSEVANMLLAEDESFLLIFKRASSLDNSVEFIKIWHQYDLDSSGFISASELKNFLKDLLSHHGR---QVEEKKLEEYTDTMMKLFDKNKDGRLDLNDMARILSLKENYLLQFSIEDASQEERRRDFEKIFAHYDVSRTGVLEGPEVDGFVKDMMELV 230 HSP 2 Score: 84.3445 bits (207), Expect = 9.973e-18 Identity = 51/179 (28.49%), Postives = 102/179 (56.98%), Query Frame = 2
Query: 4376 ATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPIS--SSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENY 4906
+ +F+ +W+ +D D++G+I A+EL NFL ++S GR+V + + T ++ +D+N+DGR+++ ++++IL E +L+ F E+ +F KI+ +D R+G +E E+ FV ++++ + + + SK F + +L+ D N DGK+Q E+A L ++ +
Sbjct: 104 SVEFIKIWHQYDLDSSGFISASELKNFL----KDLLSHH-GRQV-EEKKLEEYTDTMMKLFDKNKDGRLDLNDMARILSLKENYLLQFSIEDASQEERRRDFEKIFAHYDVSRTGVLEGPEVDGFVKDMMELVRPSLTVADLSK---FRDSLLRRCDTNQDGKIQKWELALCLGIRTAH 273
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000059.1 (pep scaffold:Pmarinus_7.0:GL485405:3181:11335:1 gene:ENSPMAG00000000055.1 transcript:ENSPMAT00000000059.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 89.3521 bits (220), Expect = 1.013e-21 Identity = 40/77 (51.95%), Postives = 59/77 (76.62%), Query Frame = 2
Query: 4508 LSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQ 4738
L +S + ++F+E YD+N DG+IE+ EL+QILPT+E FL+ F++ + SS EFM+ W+R+D DRSG+IEANEL+
Sbjct: 3 LPESVVKEKMKSFMEKYDKNADGKIEMAELAQILPTEENFLLFFRQH--VKSSAEFMETWRRYDTDRSGYIEANELK 77 HSP 2 Score: 47.3654 bits (111), Expect = 6.167e-7 Identity = 20/62 (32.26%), Postives = 42/62 (67.74%), Query Frame = 2
Query: 4835 FDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQ 5020
+D+N+DGK++++E+A++LP +EN+L + + S E ++ YD DR+G+++ E++
Sbjct: 19 YDKNADGKIEMAELAQILPTEENFL---LFFRQHVKSSAEFMETWRRYDTDRSGYIEANELK 77
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Match: CALB2 (calbindin 2 [Source:HGNC Symbol;Acc:HGNC:1435]) HSP 1 Score: 83.5741 bits (205), Expect = 7.311e-19 Identity = 50/104 (48.08%), Postives = 68/104 (65.38%), Query Frame = 2
Query: 4733 LQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
LQ+F+ +LLK K + +E K+ +T LK+FD N DGKL+LSEMARLLP++EN+L K ++ E IF YD DRNG++DE E++ALL DL E
Sbjct: 1 LQDFLKDLLK---KANREFDEKKLNEYTQTTLKMFDSNRDGKLELSEMARLLPVQENFLLKF---QGVTMTAKEFNDIFTFYDKDRNGYIDEDELEALLRDLHE 98 HSP 2 Score: 63.5438 bits (153), Expect = 7.662e-12 Identity = 34/96 (35.42%), Postives = 61/96 (63.54%), Query Frame = 2
Query: 4538 QTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIM 4825
QT L+ +D N DG++E+ E++++LP E FL+ FQ ++ EF I+ +DKDR+G+I+ +EL+ + +L + N+K+ + ++ Y NIM
Sbjct: 26 QTTLKMFDSNRDGKLELSEMARLLPVQENFLLKFQ--GVTMTAKEFNDIFTFYDKDRNGYIDEDELEALLRDLHEKNRKDLDA--QTLTSYKRNIM 117
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001806.1 (pep scaffold:Pmarinus_7.0:GL476330:305753:307416:1 gene:ENSPMAG00000001642.1 transcript:ENSPMAT00000001806.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 55.8398 bits (133), Expect = 3.846e-10 Identity = 26/54 (48.15%), Postives = 40/54 (74.07%), Query Frame = 2
Query: 4520 AFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMK 4681
+F+ + F+E +D+N DGRIE+ EL+QILPT+E FL+ F++ + SS EFM+
Sbjct: 1 SFRESMKEFMEKFDQNSDGRIELAELAQILPTEENFLLFFRQH--VKSSAEFME 52
BLAST of Calbindin-32 vs. Ensembl Nematostella
Match: EDO45965 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRJ5]) HSP 1 Score: 122.479 bits (306), Expect = 1.443e-31 Identity = 68/172 (39.53%), Postives = 105/172 (61.05%), Query Frame = 2
Query: 4343 RENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGK 4858
+ N K T+ QF D++ +D D N I+A ELD FL + + + + + A+K LE YD N DG I++ EL+Q LP +E FLV F+++N I SSV+FMKI+K++D D+SG+IE EL+ F+ +LL ++K + S+I + + M+++FDRN DGK
Sbjct: 13 KSNYAGCAKLTSEQFYDIFKKYDADGNDCIDAKELDLFL---------QDVQKADPNATNLDALKNEILEKYDANYDGVIQMSELAQCLPLEENFLVGFRKDNVI-SSVDFMKIFKKYDTDKSGYIEVPELKVFLMDLLDADKK---AISPSRINDYASAMIEIFDRNKDGK 171 HSP 2 Score: 75.0998 bits (183), Expect = 3.963e-15 Identity = 44/128 (34.38%), Postives = 81/128 (63.28%), Query Frame = 2
Query: 4661 SSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
+S +F I+K++D D + I+A EL F+ ++ K + NA ++ K N +L+ +D N DG +Q+SE+A+ LP++EN+L +N IS ++ +IFK YD D++G+++ E++ L+DL++
Sbjct: 23 TSEQFYDIFKKYDADGNDCIDAKELDLFLQDVQKADP-NATNLDALK-----NEILEKYDANYDGVIQMSELAQCLPLEENFLVGFRKDNV--ISSVDFMKIFKKYDTDKSGYIEVPELKVFLMDLLD 142
BLAST of Calbindin-32 vs. Ensembl Nematostella
Match: EDO45967 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRJ7]) HSP 1 Score: 122.865 bits (307), Expect = 9.745e-31 Identity = 80/235 (34.04%), Postives = 127/235 (54.04%), Query Frame = 2
Query: 4355 NQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSV-ISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKI----SFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
++ K + +F+ V+ FD+D NGYIE E+D F+ + EP EV + K+ LE YD ++DGR + EL ILP + L + E SV+FMKI+ RFD+D+SGF+E EL+ F ELL + K + K+ + L+ +D N DGK L+E+A++LP++EN TY I + + +++ YD D +G ++ E+ ALL D+++
Sbjct: 3 DKATKLSPVEFVKVFMKFDSDKNGYIEEKEMDLFIHDLLKELNDKEPTKAEVADK------KKEILEKYDLDKDGRFSMDELENILPVEPNLLRTYLSEGKRIGSVDFMKIFTRFDEDKSGFLETVELRGFFHELL--SMKGKSSINPRKLDDYIEYCLEKYDANKDGKFNLNELAKMLPLEENIF------ETYGIKRDMTREDFNKVWDYYDKDNSGEIEGDEILALLHDVLK 223 HSP 2 Score: 83.5741 bits (205), Expect = 2.912e-17 Identity = 57/216 (26.39%), Postives = 106/216 (49.07%), Query Frame = 2
Query: 4241 EMQKSGRINLHSGLELELEDSQKFTDNYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLL 4888
++ K GR ++ ELE+ N L Y + + + + F+ ++ FD D +G++E EL F + + G+ ++ + LE YD N+DG+ + EL+++LP +E + + ++ +F K+W +DKD SG IE +E+ + ++LK Q + E + + F +I+L+++D N D KL E+ LL
Sbjct: 66 DLDKDGRFSMD-----ELENILPVEPNLLRTYLS-----EGKRIGSVDFMKIFTRFDEDKSGFLETVELRGFFHELLSMK-----GKSSINPRKLDDYIEYCLEKYDANKDGKFNLNELAKMLPLEENIFETYGIKRDMTRE-DFNKVWDYYDKDNSGEIEGDEILALLHDVLKQQQPDRE-YNKPDLERFRDIILEIYDENKDNKLSRDELCCLL 264 HSP 3 Score: 68.9366 bits (167), Expect = 2.331e-12 Identity = 39/130 (30.00%), Postives = 76/130 (58.46%), Query Frame = 2
Query: 4661 SSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKN-NQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMEL 5047
S VEF+K++ +FD D++G+IE E+ F+ +LLK N K K E + +L+ +D + DG+ + E+ +LP++ N L + L+ +I ++ +IF +D D++GF++ E++ +L+ +
Sbjct: 9 SPVEFVKVFMKFDSDKNGYIEEKEMDLFIHDLLKELNDKEPTKAE---VADKKKEILEKYDLDKDGRFSMDELENILPVEPN-LLRTYLSEGKRIGSVDFMKIFTRFDEDKSGFLETVELRGFFHELLSM 134
BLAST of Calbindin-32 vs. Ensembl Nematostella
Match: EDO45966 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRJ6]) HSP 1 Score: 98.9821 bits (245), Expect = 1.280e-22 Identity = 65/220 (29.55%), Postives = 108/220 (49.09%), Query Frame = 2
Query: 4394 VWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTE 5053
+++ FD D NG+I+A ELD+F+ + I LS+ + K L DEN D +I EL ++LPT F+ F V+F+KIWK +DKD SGF+E ELQ F+ EL N+ ++++E + F I+L DGK + + + + + + N + + F YD D +G ++ E++ L DL+++ +
Sbjct: 24 IFSKFDKDGNGFIDAKELDSFI---------KDICNNELSEDDVASFKTGLLAKLDENNDNKISKKELRKVLPTKSNFMRQFLTTKEFCQ-VDFIKIWKNYDKDGSGFLEKPELQLFLEELFAVNKTPGQRIDEKEREKFQTILL-------DGKEGVDKKSLMKHLGVKAACDEVAANAMDWKQADFDEKFAKYDKDNSGCIESGELEVLFRDLLKIND 226 HSP 2 Score: 53.9138 bits (128), Expect = 2.104e-7 Identity = 49/178 (27.53%), Postives = 85/178 (47.75%), Query Frame = 2
Query: 4370 FTATQFLDVWNHFDTDNNGYIEAAELDNFLIQ-FATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPIS-SSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMK 4897
F F+ +W ++D D +G++E EL FL + FA +++ G+ + + + QT L E D + + L DE N + +F + + ++DKD SG IE+ EL+ +LLK N +A+ EE Y ++++ D+N D K+Q E+ L +K
Sbjct: 102 FCQVDFIKIWKNYDKDGSGFLEKPELQLFLEELFA---VNKTPGQRIDEKE--REKFQTILLDGKEGVDKKSLMKHLGVKAACDE------VAANAMDWKQADFDEKFAKYDKDNSGCIESGELEVLFRDLLKIN--DADITEEDVKDYVQSVVMPDLDKNKDNKIQKDELKSYLKIK 266
BLAST of Calbindin-32 vs. Ensembl Nematostella
Match: EDO46016 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRJ4]) HSP 1 Score: 57.3806 bits (137), Expect = 2.971e-8 Identity = 34/119 (28.57%), Postives = 61/119 (51.26%), Query Frame = 2
Query: 4682 IWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDL 5038
IW R+D +R G + +L+ F+ + +K +K+ I L D++ G++ L EMAR L +++N+L + +N K+ + RI+ YD D G + E+ AL+ D+
Sbjct: 170 IWNRYDVNREGSLSTEQLKGFINDSMK-----GQKLSSKNIGMLVKKTLDAVDKDRSGEVDLEEMARYLKVEDNFLREFNINPGQKLKQNDFSRIWDNYDQDGKGSIKGAELDALIRDV 283 HSP 2 Score: 51.6026 bits (122), Expect = 2.208e-6 Identity = 36/163 (22.09%), Postives = 78/163 (47.85%), Query Frame = 2
Query: 4394 VWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISS--SVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEM 4876
+WN +D + G + +L F I++ + + LS + + L+A D++ G +++ E+++ L ++ FL F NP +F +IW +D+D G I+ EL + ++ Q + ++++ I + +I++ D + DG + E+
Sbjct: 170 IWNRYDVNREGSLSTEQLKGF--------INDSMKGQKLSSKNIGMLVKKTLDAVDKDRSGEVDLEEMARYLKVEDNFLREFNI-NPGQKLKQNDFSRIWDNYDQDGKGSIKGAELDALIRDVFAKIQGRSPEMDD--IEKWKDIIMMFADADGDGAISKEEL 321
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: ENSORLT00000016338.2 (calretinin [Source:NCBI gene;Acc:101174993]) HSP 1 Score: 202.986 bits (515), Expect = 4.594e-58 Identity = 110/244 (45.08%), Postives = 158/244 (64.75%), Query Frame = 2
Query: 4358 QLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEEVRHSKLIMINK 5089
L + TA+QFLD+W HFD D NGYIE EL+NF + TS + S F+ + F++ +D+N DGRIE+ EL+QILPT+E FL+ F++ +SSS EFM W+R+D DRSG+IEANEL+ F+++LL +K +E K+ +T +L++FD N DGKL LSEMARLLP++EN+L K K++ + IF YD D NG++DE E++ALL DL + ++ +K +M K
Sbjct: 12 HLAEVTASQFLDIWKHFDVDGNGYIEGKELENFFRELETSRRGAGVDP---SNPQFREKMREFMQKFDKNRDGRIEMSELAQILPTEENFLLCFRQ--FVSSSAEFMAAWRRYDTDRSGYIEANELKGFLSDLL---EKANRHYDEQKLHEYTQTILRMFDLNGDGKLGLSEMARLLPVQENFLLK---FQGVKLTSEQFNSIFTYYDKDGNGYIDEQELEALLRDLYQTNKKEIDAKSLMSYK 244
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: calb2b (calretinin [Source:NCBI gene;Acc:101167606]) HSP 1 Score: 199.519 bits (506), Expect = 7.089e-57 Identity = 102/229 (44.54%), Postives = 151/229 (65.94%), Query Frame = 2
Query: 4358 QLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
QL + TA QF+D+W HFDTD NGYIE EL+NF Q ++ + S ++F+ + F+ +D+N DGRIE+ EL+QILPT+E FL+ F+ + SS EFM W+++D DRSG+IE+NEL+ F+++LLK K ++ ++ +T +L++FD N DGKL LSEMARLLP++EN+L K +++ E + +F YD D NG++DE E+ ALL DL +
Sbjct: 12 QLAELTAAQFIDIWKHFDTDGNGYIEGKELENFFTQLESARRGAGVDP---SHASFKEKMREFMANFDKNSDGRIEMSELAQILPTEENFLLCFRE--FVGSSAEFMNAWRKYDTDRSGYIESNELKGFLSDLLK---KANRIYDDKRLTEYTQTILRMFDLNGDGKLGLSEMARLLPVQENFLLKF---QGIRLTVKEFDSLFTFYDKDGNGYIDEQELDALLKDLCD 229 HSP 2 Score: 80.8777 bits (198), Expect = 5.936e-16 Identity = 43/139 (30.94%), Postives = 80/139 (57.55%), Query Frame = 2
Query: 4661 SSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNI--MLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEEVRHSK 5071
++ +F+ IWK FD D +G+IE EL+ F T+L + + V+ S + + + FD+NSDG++++SE+A++LP +EN+L + + S E ++ YD DR+G+++ E++ L DL++ + K
Sbjct: 17 TAAQFIDIWKHFDTDGNGYIEGKELENFFTQL--ESARRGAGVDPSHASFKEKMREFMANFDKNSDGRIEMSELAQILPTEENFL---LCFREFVGSSAEFMNAWRKYDTDRSGYIESNELKGFLSDLLKKANRIYDDK 150 HSP 3 Score: 75.8702 bits (185), Expect = 3.401e-14 Identity = 52/173 (30.06%), Postives = 87/173 (50.29%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSV-EFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLL 4888
++ +F++ W +DTD +GYIE+ EL FL + QT L +D N DG++ + E++++LP E FL+ FQ I +V EF ++ +DKD +G+I+ EL + +L KN V+ + +V + ++ L D GKL +EM +L
Sbjct: 108 SSAEFMNAWRKYDTDRSGYIESNELKGFLSDLLKKA------NRIYDDKRLTEYTQTILRMFDLNGDGKLGLSEMARLLPVQENFLLKFQ---GIRLTVKEFDSLFTFYDKDGNGYIDEQELDALLKDLC---DKNKMDVDATGLVDYKKSIMALSD---GGKLYRTEMEIVL 265
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: calb2b (calretinin [Source:NCBI gene;Acc:101167606]) HSP 1 Score: 188.348 bits (477), Expect = 4.516e-53 Identity = 100/233 (42.92%), Postives = 148/233 (63.52%), Query Frame = 2
Query: 4358 QLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAK----PMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
QL + TA QF+D+W HFDTD NGYIE EL+NF Q ++ + S ++F+ + F+ +D+N DGRIE+ EL+QILPT+E FL+ F+ + SS EFM W+++D DRSG+IE+NEL+ F+++LLK K ++ ++ +T +L++FD N DGKL LSEMARLLP++EN+L K P ++ T + + D NG++DE E+ ALL DL +
Sbjct: 12 QLAELTAAQFIDIWKHFDTDGNGYIEGKELENFFTQLESARRGAGVDP---SHASFKEKMREFMANFDKNSDGRIEMSELAQILPTEENFLLCFR--EFVGSSAEFMNAWRKYDTDRSGYIESNELKGFLSDLLK---KANRIYDDKRLTEYTQTILRMFDLNGDGKLGLSEMARLLPVQENFLLKFQKRPPISLTCVLVY-----------QDGNGYIDEQELDALLKDLCD 225 HSP 2 Score: 80.8777 bits (198), Expect = 5.189e-16 Identity = 43/139 (30.94%), Postives = 80/139 (57.55%), Query Frame = 2
Query: 4661 SSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNI--MLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEEVRHSK 5071
++ +F+ IWK FD D +G+IE EL+ F T+L + + V+ S + + + FD+NSDG++++SE+A++LP +EN+L + + S E ++ YD DR+G+++ E++ L DL++ + K
Sbjct: 17 TAAQFIDIWKHFDTDGNGYIEGKELENFFTQL--ESARRGAGVDPSHASFKEKMREFMANFDKNSDGRIEMSELAQILPTEENFL---LCFREFVGSSAEFMNAWRKYDTDRSGYIESNELKGFLSDLLKKANRIYDDK 150
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: ENSORLT00000006100.2 (secretagogin [Source:NCBI gene;Acc:101157482]) HSP 1 Score: 171.014 bits (432), Expect = 6.306e-47 Identity = 88/235 (37.45%), Postives = 147/235 (62.55%), Query Frame = 2
Query: 4352 NNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGEL-SQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISF-LELERIFKAYDVDRNGFMDETEMQALLVDLMELT 5050
N+ + A FL++W HFD D+NGYIE ELD F + + E ++ + +K+ F+ AYD DGR++I EL S ILP +E FL++F RE P+ +SV+FMKIW+++D D SG+I A EL+ F+ +L + + + +V K+ +T+ M+K+FD+N DG+L L+++AR+L +++N+L + ++ + K + E+IF YDV + G ++ E+ + D+MEL
Sbjct: 2 NSAFDNLDAAGFLEIWQHFDADDNGYIEGKELDEFFHHMMQRLGPQ----EETTEERVRELKERFMSAYDVTADGRLQIQELASMILPEEENFLLIFHREAPLDNSVDFMKIWRKYDADCSGYISAQELKAFLKDLFEQHHR---EVSSDKLEEYTDAMMKIFDKNKDGRLDLNDLARVLALEDNFLLQFHMDASSKEERKRDFEKIFDHYDVSKTGALEGPEVDGFVKDMMELV 229 HSP 2 Score: 76.6406 bits (187), Expect = 1.725e-14 Identity = 52/179 (29.05%), Postives = 96/179 (53.63%), Query Frame = 2
Query: 4376 ATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVE-----FMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMK 4897
+ F+ +W +D D +GYI A EL FL + E REV S + ++ +D+N+DGR+++ +L+++L ++ FL+ F + +SS E F KI+ +D ++G +E E+ FV ++++ + + E K+ ++L D N+DGK+Q +E+A L +K
Sbjct: 103 SVDFMKIWRKYDADCSGYISAQELKAFLKD-----LFEQHHREV-SSDKLEEYTDAMMKIFDKNKDGRLDLNDLARVLALEDNFLLQFHMD---ASSKEERKRDFEKIFDHYDVSKTGALEGPEVDGFVKDMMELVRPSLSGPELDKL---RAVLLHHCDVNNDGKIQKNELALCLGVK 269
BLAST of Calbindin-32 vs. Ensembl Medaka
Match: SCGN (secretagogin [Source:NCBI gene;Acc:101170527]) HSP 1 Score: 152.525 bits (384), Expect = 1.425e-40 Identity = 88/240 (36.67%), Postives = 144/240 (60.00%), Query Frame = 2
Query: 4352 NNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQI-LPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLE-----LERIFKAYDVDRNGFMDETEMQALLVDLMELTE 5053
+ L+K A FL +W +FD D+NGYIE ELD F +G + +++ Q +++ A+ + DGR+ I +L+ + LP +E FL+LF++E P+ +SVEFMKIW+++D+D SG+I ANEL+ F+ +L ++K+ + SK+ +T MLK+FD+N GKL L+++ARLL Y+ T++ E E+IF YDV + G ++ E+ L++D+MEL +
Sbjct: 2 DGALDKLDAAGFLQIWLYFDKDDNGYIEQKELDAFFKHMLLH-----LGMKDVTEDKIQELRRRC--AHRPSADGRLLIQQLASVMLPEEENFLLLFRQETPLDNSVEFMKIWRKYDEDSSGYISANELKGFLQDLFLQHRKS---ITASKLEEYTVTMLKIFDKNQGGKLDLNDLARLLL---GYVV------TFQACSREDRNRDFEKIFAHYDVSKTGALEGPEVDGLVMDMMELAK 222
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000003798.1 (SMESG000003798.1) HSP 1 Score: 548.51 bits (1412), Expect = 1.242e-180 Identity = 270/271 (99.63%), Postives = 271/271 (100.00%), Query Frame = 2
Query: 4244 MQKSGRINLHSGLELELEDSQKFTDNYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEE 5056
MQKSGRINLHSGLELELEDSQKFTDNYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQK+AEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEE
Sbjct: 1 MQKSGRINLHSGLELELEDSQKFTDNYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKSAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEE 271 HSP 2 Score: 87.4261 bits (215), Expect = 7.010e-18 Identity = 49/173 (28.32%), Postives = 96/173 (55.49%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSS-VEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLL 4888
++ +F+ +W FD D +G+IEA EL F+ + + + E + +S L+ +D N DG++++ E++++LP E +L N S +E +I+K +D DR+GF++ E+Q + +L++ + E ++ K+ F N++L +D N DGK+ + E+ +L
Sbjct: 140 SSVEFMKIWKRFDKDRSGFIEANELQEFVTEL---LKNNQKSAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTE---EPYDDKKLEKFQNVILSQWDVNKDGKISMKELKMIL 306
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000023194.1 (SMESG000023194.1) HSP 1 Score: 281.182 bits (718), Expect = 8.837e-85 Identity = 132/248 (53.23%), Postives = 188/248 (75.81%), Query Frame = 2
Query: 4313 TDNYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEE 5056
+N+L+K+R+ + N FTA QFL+VW H+DTD NGYIE ELD FL +F +SV+ IG E++S SA MK+ F+ AYDENED +IEIGEL+Q+LPTDE FL+LF+R+NP+ SSV+FMKIW++FD D SG+IE++EL+EF+ L+ ++ N +++ + K+ ++ +L+LFD N DGKLQLSEMARLLP+KENYL+KP+ N +I+ +++R+FK YD D+NG ++ E+Q LL DL+EL E
Sbjct: 8 VENFLSKFRDSKTRRWTN-FTAKQFLEVWCHYDTDGNGYIEGEELDGFLREFISSVVPGSIGSEIISDSALSDMKEDFMAAYDENEDNKIEIGELAQMLPTDENFLLLFRRDNPLESSVDFMKIWRKFDTDNSGYIESDELKEFLRYLI--SESNVKEISDEKLEEYSYTILQLFDENGDGKLQLSEMARLLPVKENYLSKPLFKNVNRITGYDIDRVFKKYDTDKNGTIENEELQGLLKDLLELAGE 252
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000055605.1 (SMESG000055605.1) HSP 1 Score: 267.7 bits (683), Expect = 3.009e-80 Identity = 129/252 (51.19%), Postives = 188/252 (74.60%), Query Frame = 2
Query: 4301 SQKFTDNYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLMELTEE 5056
+ KFT N+L +Y++ E +L FTATQF +VW+H+D D NGYIE +ELD+FL +F S++ E I + L++ +K+ FL+A+D+N D +IE+ EL+Q+LPTDE FL+LF+++ P+ SSVEFMKIWK+FDKD+SGFIEA+EL+ F++E LK + E++ E K++ +T+ ML+LFD N DGKLQLSEMARLLP+KENYL +P+ N KI+ +++E +F YD D+NG ++ E+Q L DL+ L +E
Sbjct: 3 TTKFT-NFLAQYKDNETK-KLRNFTATQFAEVWSHYDADGNGYIEGSELDSFLSEFIVSILGEDINPDELNKETLSDLKRDFLDAFDQNSDNKIEMSELAQVLPTDEVFLLLFRKDAPLDSSVEFMKIWKQFDKDQSGFIEADELKNFLSEFLK-EARPEEQILEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITTVDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQE 251 HSP 2 Score: 98.2117 bits (243), Expect = 1.023e-21 Identity = 58/183 (31.69%), Postives = 110/183 (60.11%), Query Frame = 2
Query: 4373 TATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGREVLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLV--LFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAK 4915
++ +F+ +W FD D +G+IEA EL NFL +F + E E + + T L+ +D N+DG++++ E++++LP E +L +F+ + I++ V+ ++ +D+D++G IE ELQ F+ +LL Q++ +K + +F I+L +D N DGK+ ++E+ +L M+++ LAK
Sbjct: 121 SSVEFMKIWKQFDKDQSGFIEADELKNFLSEF----LKEARPEEQILEEKLIEYTSTMLQLFDSNKDGKLQLSEMARLLPVKENYLCRPIFKNADKITT-VDIENVFHLYDRDKNGTIENEELQGFLKDLLALVQEDYDK---DDLEFFRQIILNEWDVNHDGKISMNELKMML-MQQSRLAK 294
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000043906.1 (SMESG000043906.1) HSP 1 Score: 208.764 bits (530), Expect = 3.539e-61 Identity = 107/193 (55.44%), Postives = 144/193 (74.61%), Query Frame = 2
Query: 4319 NYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGRE----VLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARL 4885
N+L+++R+ + +L FTAT+F +VWNH+D D NGYIE ELD FL +F +SV+SE E +LS++A +A+K +EAYDEN DGRIEI EL+QILP +E FL+LF+R+N + SSV+F+K+W+ FDK++SGFIEANEL+EF+ LLK K E + KI +T I+L+LFD N DGKLQLSEMARL
Sbjct: 8 NFLSQFRD-QTTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLKEFVSSVVSENFSEESKEKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANELKEFLKHLLK-EAKPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL 198 HSP 2 Score: 73.1738 bits (178), Expect = 4.530e-14 Identity = 39/135 (28.89%), Postives = 89/135 (65.93%), Query Frame = 2
Query: 4661 SSVEFMKIWKRFDKDRSGFIEANEL----QEFVTELLKNN--QKNAEKV-EESKIVYFTNIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLME 5044
++ EF ++W +DKD +G+IE +EL +EFV+ ++ N +++ EK+ E+ + N +++ +D N+DG++++ E+A++LPM+EN+L +N + S ++ ++++ +D +++GF++ E++ L L++
Sbjct: 25 TATEFAEVWNHYDKDGNGYIEGDELDLFLKEFVSSVVSENFSEESKEKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESS-VDFIKVWRMFDKNQSGFIEANELKEFLKHLLK 158
BLAST of Calbindin-32 vs. Planmine SMEST
Match: SMESG000043906.1 (SMESG000043906.1) HSP 1 Score: 204.912 bits (520), Expect = 1.243e-59 Identity = 107/204 (52.45%), Postives = 144/204 (70.59%), Query Frame = 2
Query: 4319 NYLTKYRNRENNNQLNKFTATQFLDVWNHFDTDNNGYIEAAELDNFLIQFATSVISEPIGRE---------------VLSQSAFQAMKQTFLEAYDENEDGRIEIGELSQILPTDEEFLVLFQRENPISSSVEFMKIWKRFDKDRSGFIEANELQEFVTELLKNNQKNAEKVEESKIVYFTNIMLKLFDRNSDGKLQLSEMARL 4885
N+L+++R++ +L FTAT+F +VWNH+D D NGYIE ELD FL +F +SV+SE E +LS++A +A+K +EAYDEN DGRIEI EL+QILP +E FL+LF+R+N + SSV+F+K+W+ FDK++SGFIEANEL+EF+ LLK K E + KI +T I+L+LFD N DGKLQLSEMARL
Sbjct: 8 NFLSQFRDQ-TTKKLQNFTATEFAEVWNHYDKDGNGYIEGDELDLFLKEFVSSVVSENFSEESKELNKSESQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESSVDFIKVWRMFDKNQSGFIEANELKEFLKHLLK-EAKPTEDTPKEKIDEYTKIILQLFDYNKDGKLQLSEMARL 209 HSP 2 Score: 70.8626 bits (172), Expect = 3.871e-13 Identity = 42/145 (28.97%), Postives = 88/145 (60.69%), Query Frame = 2
Query: 4661 SSVEFMKIWKRFDKDRSGFIEANEL----QEFVTELLKNN--------QKNAEKVEESKIVYFT------NIMLKLFDRNSDGKLQLSEMARLLPMKENYLAKPMLNNTYKISFLELERIFKAYDVDRNGFMDETEMQALLVDLM 5041
++ EF ++W +DKD +G+IE +EL +EFV+ ++ N K+ + E SKI+ T N +++ +D N+DG++++ E+A++LPM+EN+L +N + S ++ ++++ +D +++GF++ E++ L L+
Sbjct: 25 TATEFAEVWNHYDKDGNGYIEGDELDLFLKEFVSSVVSENFSEESKELNKSESQGESSKILSETAMKALKNEIMEAYDENNDGRIEIYELAQILPMEENFLLLFRRDNQLESS-VDFIKVWRMFDKNQSGFIEANELKEFLKHLL 168 The following BLAST results are available for this feature:
BLAST of Calbindin-32 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Calbindin-32 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Calbindin-32 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 2
BLAST of Calbindin-32 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Calbindin-32 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Calbindin-32 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Calbindin-32 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Calbindin-32 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Calbindin-32 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Calbindin-32 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 4
BLAST of Calbindin-32 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Calbindin-32 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 4
BLAST of Calbindin-32 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Calbindin-32 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30027507 ID=SMED30027507|Name=Calbindin-32|organism=Schmidtea mediterranea sexual|type=transcript|length=5656bpback to top protein sequence of SMED30027507-orf-1 >SMED30027507-orf-1 ID=SMED30027507-orf-1|Name=SMED30027507-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=295bp MQKSGRINLHSGLELELEDSQKFTDNYLTKYRNRENNNQLNKFTATQFLDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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