SMED30027498
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30027498 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Alignments
SMED30027498 aligns in the following genomic locations:
Homology
BLAST of SMED30027498 vs. Planmine SMEST
Match: SMESG000003505.1 (SMESG000003505.1) HSP 1 Score: 355.91 bits (912), Expect = 6.445e-120 Identity = 191/211 (90.52%), Postives = 192/211 (91.00%), Query Frame = 2 Query: 326 MIKCNFAMISKQIY*LLYFINDFVLVRILRISSRYLIGEIEGEFRNDVTIGIRIGFSRISDKYLMHTIDANQNKLSIKTSKSFASDSKLILNFNGATRAFKYWGFVAAFHLYIRLEFRANSLYADKWPSDELXXXXXXXXXXXXRAVTENDWPAAGDMSNRMKIWLGYDHDWISKSHSNWPTNQIIGCVELIDVNEKSIIFVKHGNDFYLD 958 MIKCNFAMI +LV I ISS YLIGEIEGEFRNDVTIGIRIGFSRISDKYLMHTIDANQNKLSIKTSKSFASDSKLILNFNGATRAFKYWGFVAAFHLYIRLEFRANSLYA KWPSDELIISNISYSSISSRAVTENDWPAAGDMSNRMKIWLGYDHDWISKSHSNWPTNQIIGCVELIDVNEKSIIFVKHGNDFYLD Sbjct: 1 MIKCNFAMI--------------ILVNIFGISSSYLIGEIEGEFRNDVTIGIRIGFSRISDKYLMHTIDANQNKLSIKTSKSFASDSKLILNFNGATRAFKYWGFVAAFHLYIRLEFRANSLYAAKWPSDELIISNISYSSISSRAVTENDWPAAGDMSNRMKIWLGYDHDWISKSHSNWPTNQIIGCVELIDVNEKSIIFVKHGNDFYLD 197
BLAST of SMED30027498 vs. Planmine SMEST
Match: SMESG000003505.1 (SMESG000003505.1) HSP 1 Score: 346.28 bits (887), Expect = 2.801e-116 Identity = 182/188 (96.81%), Postives = 183/188 (97.34%), Query Frame = 2 Query: 395 VLVRILRISSRYLIGEIEGEFRNDVTIGIRIGFSRISDKYLMHTIDANQNKLSIKTSKSFASDSKLILNFNGATRAFKYWGFVAAFHLYIRLEFRANSLYADKWPSDELXXXXXXXXXXXXRAVTENDWPAAGDMSNRMKIWLGYDHDWISKSHSNWPTNQIIGCVELIDVNEKSIIFVKHGNDFYLD 958 +LV I ISS YLIGEIEGEFRNDVTIGIRIGFSRISDKYLMHTIDANQNKLSIKTSKSFASDSKLILNFNGATRAFKYWGFVAAFHLYIRLEFRANSLYA KWPSDELIISNISYSSISSRAVTENDWPAAGDMSNRMKIWLGYDHDWISKSHSNWPTNQIIGCVELIDVNEKSIIFVKHGNDFYLD Sbjct: 3 ILVNIFGISSSYLIGEIEGEFRNDVTIGIRIGFSRISDKYLMHTIDANQNKLSIKTSKSFASDSKLILNFNGATRAFKYWGFVAAFHLYIRLEFRANSLYAAKWPSDELIISNISYSSISSRAVTENDWPAAGDMSNRMKIWLGYDHDWISKSHSNWPTNQIIGCVELIDVNEKSIIFVKHGNDFYLD 190
BLAST of SMED30027498 vs. Planmine SMEST
Match: SMESG000003492.1 (SMESG000003492.1) HSP 1 Score: 323.168 bits (827), Expect = 8.551e-104 Identity = 179/211 (84.83%), Postives = 185/211 (87.68%), Query Frame = 2 Query: 326 MIKCNFAMISKQIY*LLYFINDFVLVRILRISSRYLIGEIEGEFRNDVTIGIRIGFSRISDKYLMHTIDANQNKLSIKTSKSFASDSKLILNFNGATRAFKYWGFVAAFHLYIRLEFRANSLYADKWPSDELXXXXXXXXXXXXRAVTENDWPAAGDMSNRMKIWLGYDHDWISKSHSNWPTNQIIGCVELIDVNEKSIIFVKHGNDFYLD 958 MIKCNFA+ISKQIY LYFIN+ VLV I ISS YLIGEIEGEFRNDVTIGIRIGFSRISDKYL+H IDANQNKLSIKTSKSFASDSKLILNFNGATRAFK LE NSLYA KWPSDELIISNISYSSI+SR+VTENDWPAAGDMSNRMKIWLGYDHDWISKSHSNWPTNQIIGCVELIDVN KSIIFVKHGNDFY+D Sbjct: 1 MIKCNFALISKQIY-YLYFINNLVLVSIFGISSSYLIGEIEGEFRNDVTIGIRIGFSRISDKYLLHAIDANQNKLSIKTSKSFASDSKLILNFNGATRAFKC------------LE---NSLYAAKWPSDELIISNISYSSINSRSVTENDWPAAGDMSNRMKIWLGYDHDWISKSHSNWPTNQIIGCVELIDVNGKSIIFVKHGNDFYMD 195 HSP 2 Score: 146.747 bits (369), Expect = 9.488e-38 Identity = 71/86 (82.56%), Postives = 74/86 (86.05%), Query Frame = 2 Query: 902 DVNEKSIIFVKHGNDFYLDRISNGYFIKTRMLPNQFRVETINIGWNSNPYLCQPIRGKFGIQLNSNLKSAVKWDASRCPKVPQINA 1159 DV+ S+ F+ H RISNGYFIKTRMLPN FRVETINIGWNSNPYLCQPIRGKFGIQLNSNLKSAVKWDASRCPKVPQINA Sbjct: 317 DVHALSMGFIDHKLTI---RISNGYFIKTRMLPNHFRVETINIGWNSNPYLCQPIRGKFGIQLNSNLKSAVKWDASRCPKVPQINA 399 HSP 3 Score: 137.502 bits (345), Expect = 1.337e-34 Identity = 70/93 (75.27%), Postives = 77/93 (82.80%), Query Frame = 3 Query: 1479 FLSPFLWCSIIINISLMFILLLIIGKRIIDHWRHKELASQNSNVYEEQQQDLNELNLKSLAIHRLGIGENVDGGHRGTANVTNSASDPQDETN 1757 FL+PFLW SIIINI+LM ILLLIIGKRIIDH KE+ +NSNVYE+Q Q+L +NL SLAI RLGIGENVDGGHR TANVT ASDPQDE N Sbjct: 412 FLNPFLWSSIIINIALMSILLLIIGKRIIDHRHRKEITRRNSNVYEQQMQELIGINLTSLAIQRLGIGENVDGGHRRTANVTIIASDPQDEAN 504
BLAST of SMED30027498 vs. Planmine SMEST
Match: SMESG000003492.1 (SMESG000003492.1) HSP 1 Score: 297.745 bits (761), Expect = 3.852e-94 Identity = 168/211 (79.62%), Postives = 174/211 (82.46%), Query Frame = 2 Query: 326 MIKCNFAMISKQIY*LLYFINDFVLVRILRISSRYLIGEIEGEFRNDVTIGIRIGFSRISDKYLMHTIDANQNKLSIKTSKSFASDSKLILNFNGATRAFKYWGFVAAFHLYIRLEFRANSLYADKWPSDELXXXXXXXXXXXXRAVTENDWPAAGDMSNRMKIWLGYDHDWISKSHSNWPTNQIIGCVELIDVNEKSIIFVKHGNDFYLD 958 MIKCNFA+I +LV I ISS YLIGEIEGEFRNDVTIGIRIGFSRISDKYL+H IDANQNKLSIKTSKSFASDSKLILNFNGATRAFK LE NSLYA KWPSDELIISNISYSSI+SR+VTENDWPAAGDMSNRMKIWLGYDHDWISKSHSNWPTNQIIGCVELIDVN KSIIFVKHGNDFY+D Sbjct: 1 MIKCNFALI--------------ILVSIFGISSSYLIGEIEGEFRNDVTIGIRIGFSRISDKYLLHAIDANQNKLSIKTSKSFASDSKLILNFNGATRAFKC------------LE---NSLYAAKWPSDELIISNISYSSINSRSVTENDWPAAGDMSNRMKIWLGYDHDWISKSHSNWPTNQIIGCVELIDVNGKSIIFVKHGNDFYMD 182 HSP 2 Score: 146.747 bits (369), Expect = 8.318e-38 Identity = 71/86 (82.56%), Postives = 74/86 (86.05%), Query Frame = 2 Query: 902 DVNEKSIIFVKHGNDFYLDRISNGYFIKTRMLPNQFRVETINIGWNSNPYLCQPIRGKFGIQLNSNLKSAVKWDASRCPKVPQINA 1159 DV+ S+ F+ H RISNGYFIKTRMLPN FRVETINIGWNSNPYLCQPIRGKFGIQLNSNLKSAVKWDASRCPKVPQINA Sbjct: 304 DVHALSMGFIDHKLTI---RISNGYFIKTRMLPNHFRVETINIGWNSNPYLCQPIRGKFGIQLNSNLKSAVKWDASRCPKVPQINA 386 HSP 3 Score: 137.502 bits (345), Expect = 1.291e-34 Identity = 70/93 (75.27%), Postives = 77/93 (82.80%), Query Frame = 3 Query: 1479 FLSPFLWCSIIINISLMFILLLIIGKRIIDHWRHKELASQNSNVYEEQQQDLNELNLKSLAIHRLGIGENVDGGHRGTANVTNSASDPQDETN 1757 FL+PFLW SIIINI+LM ILLLIIGKRIIDH KE+ +NSNVYE+Q Q+L +NL SLAI RLGIGENVDGGHR TANVT ASDPQDE N Sbjct: 399 FLNPFLWSSIIINIALMSILLLIIGKRIIDHRHRKEITRRNSNVYEQQMQELIGINLTSLAIQRLGIGENVDGGHRRTANVTIIASDPQDEAN 491
BLAST of SMED30027498 vs. Planmine SMEST
Match: SMESG000003492.1 (SMESG000003492.1) HSP 1 Score: 280.026 bits (715), Expect = 3.430e-89 Identity = 149/211 (70.62%), Postives = 153/211 (72.51%), Query Frame = 2 Query: 326 MIKCNFAMISKQIY*LLYFINDFVLVRILRISSRYLIGEIEGEFRNDVTIGIRIGFSRISDKYLMHTIDANQNKLSIKTSKSFASDSKLILNFNGATRAFKYWGFVAAFHLYIRLEFRANSLYADKWPSDELXXXXXXXXXXXXRAVTENDWPAAGDMSNRMKIWLGYDHDWISKSHSNWPTNQIIGCVELIDVNEKSIIFVKHGNDFYLD 958 MIKCNFA+ISKQIY LYFIN+ VLV I ISS YLIGEIEGEFRNDVTIGIRIGFSRISDKYL+H IDANQNKLSIKTSKSFASDSKLILNFNGATRAFKYW PAAGDMSNRMKIWLGYDHDWISKSHSNWPTNQIIGCVELIDVN KSIIFVKHGNDFY+D Sbjct: 1 MIKCNFALISKQIY-YLYFINNLVLVSIFGISSSYLIGEIEGEFRNDVTIGIRIGFSRISDKYLLHAIDANQNKLSIKTSKSFASDSKLILNFNGATRAFKYW-------------------------------------------------PAAGDMSNRMKIWLGYDHDWISKSHSNWPTNQIIGCVELIDVNGKSIIFVKHGNDFYMD 161 HSP 2 Score: 93.2041 bits (230), Expect = 2.009e-20 Identity = 45/60 (75.00%), Postives = 48/60 (80.00%), Query Frame = 2 Query: 902 DVNEKSIIFVKHGNDFYLDRISNGYFIKTRMLPNQFRVETINIGWNSNPYLCQPIRGKFG 1081 DV+ S+ F+ H RISNGYFIKTRMLPN FRVETINIGWNSNPYLCQPIRGKFG Sbjct: 283 DVHALSMGFIDHKLTI---RISNGYFIKTRMLPNHFRVETINIGWNSNPYLCQPIRGKFG 339 The following BLAST results are available for this feature:
BLAST of SMED30027498 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30027498 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30027498 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30027498 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30027498 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30027498 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30027498 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30027498 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30027498 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30027498 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30027498 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30027498 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30027498 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30027498 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30027498 ID=SMED30027498|Name=SMED30027498|organism=Schmidtea mediterranea sexual|type=transcript|length=1757bpback to top protein sequence of SMED30027498-orf-1 >SMED30027498-orf-1 ID=SMED30027498-orf-1|Name=SMED30027498-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=234bp MHTIDANQNKLSIKTSKSFASDSKLILNFNGATRAFKYWGFVAAFHLYIRback to top Annotated Terms
The following terms have been associated with this transcript:
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