SMED30027478

Overview
NameSMED30027478
Smed IDSMED30027478
Length (bp)1354
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30027478 (SMED30027478) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30027478 (SMED30027478) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30027478 (SMED30027478) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30027478 (SMED30027478) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30027478

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30027478h1SMcG0021852 SmedASXL_019337SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
pharynxSMED30027478h1SMcG0021852 dd_Smed_v4_3287_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30027478h1SMcG0021852 dd_Smed_v4_3287_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30027478h1SMcG0021852 dd_Smed_v4_3287_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30027478h1SMcG0021852 dd_Smed_v4_3287_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30027478h1SMcG0021852 dd_Smed_v4_3287_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30027478h1SMcG0021852 dd_Smed_v4_3287_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
Smed sexual biotypeSMED30027478h1SMcG0021852 Contig49136newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027478h1SMcG0021852 Contig49136uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30027478h1SMcG0021852 dd_Smed_v6_3287_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
epidermisSMED30027478h1SMcG0021852 dd_Smed_v4_3287_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30027478h1SMcG0021852 dd_Smed_v4_3287_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30027478 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.015366:1..11674 +6087
Homology
BLAST of SMED30027478 vs. Ensembl Human
Match: TEKT1 (tektin 1 [Source:HGNC Symbol;Acc:HGNC:15534])

HSP 1 Score: 333.183 bits (853), Expect = 2.439e-110
Identity = 181/414 (43.72%), Postives = 267/414 (64.49%), Query Frame = -3
Query:   60 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKI 1301
            MA L++PP ++   EW  +N  +Y  A+ +R  +ERL  E  RL+DE +K T KS +DVNKK++QR++++++WK ELD KL  +      L  +  R+ KA E L EPLHI++ C+A RE R+ IDLVHD V+ ELIKE E+  G+  LL RT+E+A EQ+R+NR A+Y L++D+ DK  A  ID+    L  NS +I Y+  A  I+P +V+ EDW  F++ N+   D+Q  NS  +++++D +L QTA+D ++Q   VDTAF+  + + +D++ KL +HLA+V++EI++ E  I  LE AI  +  P K+A TRL+ R  RPNVE CRD  QY      + +V  IT            ++A L+  LA A +E K L RRQL L+EEIQVK+N+++IDEV CM +R+SI +
Sbjct:    1 MAKLLQPPPKFLPSEWHIANKNQYHRADAQRSRSERLVAESQRLVDEIEKTTRKSQSDVNKKLEQRLEEVQFWKKELDDKLEQLVNVTDDLLIYKIRLEKALETLKEPLHITETCLAYREKRIGIDLVHDTVEHELIKEAEIIQGIMALLTRTLEEASEQIRMNRSAKYNLEKDLKDKFVALTIDDICFSLNNNSPNIRYSENAVRIEPNSVSLEDWLDFSSTNVEKADKQRNNSLMLKALVDRILSQTANDLRKQCDVVDTAFKNGLKDTKDARDKLADHLAKVMEEIASQEKNITALEKAILDQEGPAKVAHTRLETRTHRPNVELCRDVAQY----RLMKEVQEIT-----------HNVARLKETLAQAQAELKGLHRRQLALQEEIQVKENTIYIDEVLCMQMRKSIPL 399          
BLAST of SMED30027478 vs. Ensembl Human
Match: TEKT3 (tektin 3 [Source:HGNC Symbol;Acc:HGNC:14293])

HSP 1 Score: 229.18 bits (583), Expect = 3.665e-69
Identity = 138/402 (34.33%), Postives = 229/402 (56.97%), Query Frame = -3
Query:   69 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRES 1274
            RYT  +W  SN T Y+ +   R  +E+L+ +  RLI +  ++T K+  D  + + +R++DI +WK+E+  +L ++  E   L +  KR+ +A      PL ++++C+ +RE R+ IDLVHD+V+ +L+ EV+     +E +   +++A+ QL  NR +++ L++D+ DK TA  ID+    L+  S  +GY      +D     PE W  FT++NIL    +   S+ +R  I+ +L  TA++   Q   V+ +F  RI E  D+K K++ HLA+ L+EI   E TI++++ AI+ K   LK+AQTRL  R  RPN+E CRD  Q     + + +VD+               I  LQ+RL DA    ++L   +  LE ++ VK NSL+ID+ +CM +R+S
Sbjct:   93 RYTPDDWYRSNLTNYQESNTSRHNSEKLRVDTSRLIQDKYQQTRKTQADTTQNLGERVNDIGFWKSEIIHELDEMIGETNALTDVKKRLERALMETEAPLQVARECLFHREKRMGIDLVHDEVEAQLLTEVDTILCCQERMKLHLDKAIAQLAANRASQHELEKDLSDKQTAYRIDDKCHHLRNTSDGVGYFRGVERVDATVSVPESWAKFTDDNILRSQSERAASAKLRDDIENLLVVTANEMWNQFNKVNLSFTNRIAETADAKNKIQTHLAKTLQEIFQTEMTIESIKKAIKDKTAFLKVAQTRLDERTRRPNIELCRDMAQL-RLVNEVHEVDDT--------------IQTLQQRLRDAEDTLQSLVHIKATLEYDLAVKANSLYIDQEKCMSMRKS 479          
BLAST of SMED30027478 vs. Ensembl Human
Match: TEKT3 (tektin 3 [Source:HGNC Symbol;Acc:HGNC:14293])

HSP 1 Score: 229.18 bits (583), Expect = 3.665e-69
Identity = 138/402 (34.33%), Postives = 229/402 (56.97%), Query Frame = -3
Query:   69 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRES 1274
            RYT  +W  SN T Y+ +   R  +E+L+ +  RLI +  ++T K+  D  + + +R++DI +WK+E+  +L ++  E   L +  KR+ +A      PL ++++C+ +RE R+ IDLVHD+V+ +L+ EV+     +E +   +++A+ QL  NR +++ L++D+ DK TA  ID+    L+  S  +GY      +D     PE W  FT++NIL    +   S+ +R  I+ +L  TA++   Q   V+ +F  RI E  D+K K++ HLA+ L+EI   E TI++++ AI+ K   LK+AQTRL  R  RPN+E CRD  Q     + + +VD+               I  LQ+RL DA    ++L   +  LE ++ VK NSL+ID+ +CM +R+S
Sbjct:   93 RYTPDDWYRSNLTNYQESNTSRHNSEKLRVDTSRLIQDKYQQTRKTQADTTQNLGERVNDIGFWKSEIIHELDEMIGETNALTDVKKRLERALMETEAPLQVARECLFHREKRMGIDLVHDEVEAQLLTEVDTILCCQERMKLHLDKAIAQLAANRASQHELEKDLSDKQTAYRIDDKCHHLRNTSDGVGYFRGVERVDATVSVPESWAKFTDDNILRSQSERAASAKLRDDIENLLVVTANEMWNQFNKVNLSFTNRIAETADAKNKIQTHLAKTLQEIFQTEMTIESIKKAIKDKTAFLKVAQTRLDERTRRPNIELCRDMAQL-RLVNEVHEVDDT--------------IQTLQQRLRDAEDTLQSLVHIKATLEYDLAVKANSLYIDQEKCMSMRKS 479          
BLAST of SMED30027478 vs. Ensembl Human
Match: TEKT5 (tektin 5 [Source:HGNC Symbol;Acc:HGNC:26554])

HSP 1 Score: 224.557 bits (571), Expect = 2.140e-67
Identity = 141/403 (34.99%), Postives = 222/403 (55.09%), Query Frame = -3
Query:   66 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESI 1274
            RY+  +W  SN  + R AE  R  A RL ++  RL+ + D+ T +      + + QR+ DI +WK+EL  +L  +  EN  L+   +R+  A   +  PL ++ +C+ +RE R+ IDLVHD+V+K LI+EV++    +E + +  ++   Q+R NR A++ L+RD++DK +AQ IDE    L+  S  I + +   +ID     PE W  F+N+NI +      NS  +R   + + +  +D   RQ    + AF  RI+EV D K KL+  LA+ L+EI   E+TI  LE +I AK  PLK+AQTRL+ R  RPN+E CRD PQ       + +V  I           +  +  L+ RL +     + L   +  LE E+ +K N+L ID+ +CMG+R++ 
Sbjct:   88 RYSPHDWDQSNQLQVRGAEASRLWASRLTDDSMRLLQDKDQLTHQMQEGTCRNLGQRLSDIGFWKSELSYELDRLLTENQNLETVKRRLECAANEVNCPLQVALECLYHREKRIGIDLVHDNVEKNLIREVDLLKCCQEQMRKLAQRIDIQMRDNRDAQHVLERDLEDKSSAQCIDEKCFNLRNTSDCISFFHGMEKIDGTISVPETWAKFSNDNIKHSQNMRANSIQLREEAEHLFETLSDQMWRQFTDTNLAFNARISEVTDVKNKLQTQLAKTLQEIFQAENTIMLLERSIMAKEGPLKVAQTRLECRTRRPNMELCRDIPQL----KLVNEVFTI-----------DDTLQTLKLRLRETQDTLQLLVMTKCRLEHELAIKANTLCIDKEKCMGMRKTF 475          
BLAST of SMED30027478 vs. Ensembl Human
Match: TEKT4 (tektin 4 [Source:HGNC Symbol;Acc:HGNC:31012])

HSP 1 Score: 203.371 bits (516), Expect = 5.995e-60
Identity = 130/400 (32.50%), Postives = 212/400 (53.00%), Query Frame = -3
Query:   75 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIR 1274
            +Y   EW  +   +Y  A  +R  +ER ++E  +L  ET     ++  D  + + +R+ D   WK+EL +++  +A E   L    +R+ +A +A   P  I+   +  RE R   +LV D V+ EL+KE E+   ++ELL RTI QAV Q+RLNR+ +   + D  DK+ A  IDE        S ++     +      A TPE    FT +N+    R+ L S+++R ++D +L+ T++D + Q  AV+ AF RR  E+ D++ KL  HL + L+EI++ E  +  L+ AI+ K  PL +AQTRL +R  RPN+E CRD  Q+      + +V+ + +            +  L+ +L +A    + L+   + LE++I    NSLFID  +CM  R
Sbjct:   38 KYLLEEWFQNCYARYHQAFADRDQSERQRHESQQLATETQALAQRTQQDSTRTVGERLQDTHSWKSELQREMEALAAETNLLLAQKQRLERALDATEVPFSITTDNLQCRERREHPNLVRDHVETELLKEAELIRNIQELLKRTIMQAVSQIRLNREHKETCEMDWSDKMEAYNIDETCGRHHSQSTEVQAHPYSTTFQESASTPETRAKFTQDNLCRAQRERLASANLRVLVDCILRDTSEDLRLQCDAVNLAFGRRCEELEDARYKLHHHLHKTLREITDQEHNVAALKQAIKDKEAPLHVAQTRLYLRSHRPNMELCRDAAQF----RLLSEVEELNM-----------SLTALREKLLEAEQSLRNLEDIHMSLEKDIAAMTNSLFIDRQKCMAHR 422          
BLAST of SMED30027478 vs. Ensembl Celegans
Match: R02E12.4 (pep chromosome:WBcel235:X:4005314:4007634:1 gene:WBGene00019828.1 transcript:R02E12.4.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:R02E12.4)

HSP 1 Score: 77.411 bits (189), Expect = 3.139e-15
Identity = 72/301 (23.92%), Postives = 133/301 (44.19%), Query Frame = -3
Query:  294 IDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAI-DLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKP-NSKDIGYTNAAAEIDPKAVTP-------EDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYS 1169
            + E + R  +      +++ QR+ D+KYW  E+D++LADV  E+  L    +R+    +        ++ C+A R  +V + D   D V  EL KE E+     + +    E+   QL +N+ A   L RD+  K  A  +D     +    +K +  T     +D +   P        +W   T  N+    R  ++S  +   +   +++ A   + + IAV+   +  +   ++ K  L   +    KEI N +  I+ +  ++  K  PL++A +R   R  RP +E C D  Q++
Sbjct:   21 VKEANMRAARMQGQTTQQLMQRVKDLKYWSGEIDRELADVKEEHDDLLRCYRRLQLCLDITGRSSRCNESCLAVRRKKVQVGDHTSDRVDMELHKEKELMTDTMKQMKEIGERVERQLEVNQSARKNLLRDLTLKQEAISLDHKSVAIGADGTKTVIRTPDGDRLDFREGAPLQRMSEYSEWIENTGNNLNYAARARVHSRKIGQKLCQSIREMAHQLRTEAIAVEALLKDSVRNWQEWKDNLHSQVNGKDKEIKNADGAIEEISFSLRQKSGPLQVALSRQNQRGLRPGIELCNDKAQHA 321          
BLAST of SMED30027478 vs. Ensembl Fly
Match: CG32819 (gene:FBgn0052819 transcript:FBtr0070051)

HSP 1 Score: 189.119 bits (479), Expect = 4.143e-54
Identity = 122/405 (30.12%), Postives = 213/405 (52.59%), Query Frame = -3
Query:   60 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKI 1274
            RYT  EW  +N TKY  + + R  +ER++N+  RL+ ETD++      D  +++ +RI D+ +W+NEL+ +L  +  E   ++E  ++ GKA   L  PLHI+++C+ +RE R   + VHD V+K L+ E+      ++ L    E+  +Q    R A++ L+ D+  K ++ GID    +L  +S+ I Y     + DP   T E W   ++E++     +    S +RS    V+   A          + AF RR  E+ ++K +++ HL +V +E+ ++E  +  L+ AI+ K  PLK+AQTRL+ R  R  VE C+D+ Q       + +V +I           +  +  L  +L +A +  + L + +  LE +++ K N+LFID  +CM +R S  +
Sbjct:  125 RYTSNEWYNNNMTKYSESNMNRNLSERMRNDAVRLMRETDEKATSGQRDAGRRLGERITDLTFWRNELNAELEKLIAEMSDINELQRQCGKALLDLEIPLHIAQECLFHRESRQGTEKVHDIVEKALLVEINNLRNSRDRLGGLHEKISKQALDCRGAQHLLEDDVSHKESSLGIDSMCHQLNNHSRGITYYGGIEKFDPSVSTQESWAQASSEHVRRSQAERAKLSQLRSDAQSVVNSVATTVWDFWSNTNNAFDRRSQEMAEAKNRVQLHLQKVQQELFDMEKHLFLLQKAIQDKSGPLKVAQTRLEARSHREGVELCKDHAQD----RLVQEVQDI-----------QGAVETLHHKLMEAEATHQGLLKTRCTLEVDLRNKVNALFIDREKCMSLRRSFPV 514          
BLAST of SMED30027478 vs. Ensembl Fly
Match: CG32820 (gene:FBgn0052820 transcript:FBtr0070054)

HSP 1 Score: 189.119 bits (479), Expect = 4.143e-54
Identity = 122/405 (30.12%), Postives = 213/405 (52.59%), Query Frame = -3
Query:   60 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKI 1274
            RYT  EW  +N TKY  + + R  +ER++N+  RL+ ETD++      D  +++ +RI D+ +W+NEL+ +L  +  E   ++E  ++ GKA   L  PLHI+++C+ +RE R   + VHD V+K L+ E+      ++ L    E+  +Q    R A++ L+ D+  K ++ GID    +L  +S+ I Y     + DP   T E W   ++E++     +    S +RS    V+   A          + AF RR  E+ ++K +++ HL +V +E+ ++E  +  L+ AI+ K  PLK+AQTRL+ R  R  VE C+D+ Q       + +V +I           +  +  L  +L +A +  + L + +  LE +++ K N+LFID  +CM +R S  +
Sbjct:  125 RYTSNEWYNNNMTKYSESNMNRNLSERMRNDAVRLMRETDEKATSGQRDAGRRLGERITDLTFWRNELNAELEKLIAEMSDINELQRQCGKALLDLEIPLHIAQECLFHRESRQGTEKVHDIVEKALLVEINNLRNSRDRLGGLHEKISKQALDCRGAQHLLEDDVSHKESSLGIDSMCHQLNNHSRGITYYGGIEKFDPSVSTQESWAQASSEHVRRSQAERAKLSQLRSDAQSVVNSVATTVWDFWSNTNNAFDRRSQEMAEAKNRVQLHLQKVQQELFDMEKHLFLLQKAIQDKSGPLKVAQTRLEARSHREGVELCKDHAQD----RLVQEVQDI-----------QGAVETLHHKLMEAEATHQGLLKTRCTLEVDLRNKVNALFIDREKCMSLRRSFPV 514          
BLAST of SMED30027478 vs. Ensembl Fly
Match: CG17450 (gene:FBgn0040028 transcript:FBtr0345920)

HSP 1 Score: 189.119 bits (479), Expect = 4.143e-54
Identity = 122/405 (30.12%), Postives = 213/405 (52.59%), Query Frame = -3
Query:   60 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKI 1274
            RYT  EW  +N TKY  + + R  +ER++N+  RL+ ETD++      D  +++ +RI D+ +W+NEL+ +L  +  E   ++E  ++ GKA   L  PLHI+++C+ +RE R   + VHD V+K L+ E+      ++ L    E+  +Q    R A++ L+ D+  K ++ GID    +L  +S+ I Y     + DP   T E W   ++E++     +    S +RS    V+   A          + AF RR  E+ ++K +++ HL +V +E+ ++E  +  L+ AI+ K  PLK+AQTRL+ R  R  VE C+D+ Q       + +V +I           +  +  L  +L +A +  + L + +  LE +++ K N+LFID  +CM +R S  +
Sbjct:  125 RYTSNEWYNNNMTKYSESNMNRNLSERMRNDAVRLMRETDEKATSGQRDAGRRLGERITDLTFWRNELNAELEKLIAEMSDINELQRQCGKALLDLEIPLHIAQECLFHRESRQGTEKVHDIVEKALLVEINNLRNSRDRLGGLHEKISKQALDCRGAQHLLEDDVSHKESSLGIDSMCHQLNNHSRGITYYGGIEKFDPSVSTQESWAQASSEHVRRSQAERAKLSQLRSDAQSVVNSVATTVWDFWSNTNNAFDRRSQEMAEAKNRVQLHLQKVQQELFDMEKHLFLLQKAIQDKSGPLKVAQTRLEARSHREGVELCKDHAQD----RLVQEVQDI-----------QGAVETLHHKLMEAEATHQGLLKTRCTLEVDLRNKVNALFIDREKCMSLRRSFPV 514          
BLAST of SMED30027478 vs. Ensembl Fly
Match: CG17450 (gene:FBgn0040028 transcript:FBtr0070056)

HSP 1 Score: 189.119 bits (479), Expect = 4.143e-54
Identity = 122/405 (30.12%), Postives = 213/405 (52.59%), Query Frame = -3
Query:   60 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKI 1274
            RYT  EW  +N TKY  + + R  +ER++N+  RL+ ETD++      D  +++ +RI D+ +W+NEL+ +L  +  E   ++E  ++ GKA   L  PLHI+++C+ +RE R   + VHD V+K L+ E+      ++ L    E+  +Q    R A++ L+ D+  K ++ GID    +L  +S+ I Y     + DP   T E W   ++E++     +    S +RS    V+   A          + AF RR  E+ ++K +++ HL +V +E+ ++E  +  L+ AI+ K  PLK+AQTRL+ R  R  VE C+D+ Q       + +V +I           +  +  L  +L +A +  + L + +  LE +++ K N+LFID  +CM +R S  +
Sbjct:  125 RYTSNEWYNNNMTKYSESNMNRNLSERMRNDAVRLMRETDEKATSGQRDAGRRLGERITDLTFWRNELNAELEKLIAEMSDINELQRQCGKALLDLEIPLHIAQECLFHRESRQGTEKVHDIVEKALLVEINNLRNSRDRLGGLHEKISKQALDCRGAQHLLEDDVSHKESSLGIDSMCHQLNNHSRGITYYGGIEKFDPSVSTQESWAQASSEHVRRSQAERAKLSQLRSDAQSVVNSVATTVWDFWSNTNNAFDRRSQEMAEAKNRVQLHLQKVQQELFDMEKHLFLLQKAIQDKSGPLKVAQTRLEARSHREGVELCKDHAQD----RLVQEVQDI-----------QGAVETLHHKLMEAEATHQGLLKTRCTLEVDLRNKVNALFIDREKCMSLRRSFPV 514          
BLAST of SMED30027478 vs. Ensembl Fly
Match: CG32819 (gene:FBgn0052819 transcript:FBtr0070050)

HSP 1 Score: 188.734 bits (478), Expect = 2.661e-53
Identity = 122/405 (30.12%), Postives = 212/405 (52.35%), Query Frame = -3
Query:   60 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKI 1274
            RYT  EW  +N TKY  + + R  +ER++N+  RL+ ETD++      D  +++ +RI D+ +W+NEL+ +L  +  E   ++E  ++ GKA   L  PLHI+++C+ +RE R   + VHD V+K L+ E+      ++ L    E+  +Q    R A++ L+ D+  K ++ GID    +L  +S+ I Y     + DP   T E W   ++E++     +    S +RS    V+   A          + AF RR  E+ ++K +++ HL +V +E+ ++E  +  L+ AI+ K  PLK+AQTRL+ R  R  VE C+D+ Q       + +V +I              +  L  +L +A +  + L + +  LE +++ K N+LFID  +CM +R S  +
Sbjct:  206 RYTSNEWYNNNMTKYSESNMNRNLSERMRNDAVRLMRETDEKATSGQRDAGRRLGERITDLTFWRNELNAELEKLIAEMSDINELQRQCGKALLDLEIPLHIAQECLFHRESRQGTEKVHDIVEKALLVEINNLRNSRDRLGGLHEKISKQALDCRGAQHLLEDDVSHKESSLGIDSMCHQLNNHSRGITYYGGIEKFDPSVSTQESWAQASSEHVRRSQAERAKLSQLRSDAQSVVNSVATTVWDFWSNTNNAFDRRSQEMAEAKNRVQLHLQKVQQELFDMEKHLFLLQKAIQDKSGPLKVAQTRLEARSHREGVELCKDHAQD----RLVQEVQDIQGA-----------VETLHHKLMEAEATHQGLLKTRCTLEVDLRNKVNALFIDREKCMSLRRSFPV 595          
BLAST of SMED30027478 vs. Ensembl Zebrafish
Match: tekt1 (tektin 1 [Source:ZFIN;Acc:ZDB-GENE-041114-99])

HSP 1 Score: 289.656 bits (740), Expect = 1.104e-93
Identity = 173/417 (41.49%), Postives = 249/417 (59.71%), Query Frame = -3
Query:   51 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKIEAY 1301
            M+ L+ PP R+   EW ++N   YR++E  R+ +E+L  EC+RLI+E +K   +  +D  K++++RI DIK+W+ EL +K   +  E   L  +  RV KA E+  EP  ++ QC+A R+ RVAIDLVHD+V++EL+KE EV  GV  LL RT+EQ  EQ+RL R  +Y L++D+ DK  A+ ID+F   L   S D+   N++ +    AVTPE+W+S  N NI   +++  NS  +R+++D +L+QTA D +RQ      AF  RI E + +K +LE  L  +L EI+N E     L +A+  K  PLK+AQTRL  R  RPNVE C D PQ       + Q+  +T             +  L   L  + SE KAL   QL LEEEIQVK NSL+IDEV C  +R+ I I+++
Sbjct:    1 MSRLMGPPDRFLPSEWKHANQEHYRSSEAGRKHSEKLTAECERLIEECNKSNKRMQHDTQKRLERRIQDIKFWRQELVQKFEQMVQEIETLIIYKSRVEKALESCSEPFQVTLQCLAERQKRVAIDLVHDEVEEELLKEKEVVEGVMVLLQRTLEQINEQIRLIRSVKYYLEKDLQDKFQAEHIDDFCSLLTNTSPDLNEGNSSFKYSGAAVTPEEWESLCNLNISKAEKEKNNSLSLRALVDSLLEQTARDMRRQYETTGRAFDLRIEETKTAKEQLENQLNELLAEIANQEKNQDALRLAVAEKEAPLKVAQTRLSTRSQRPNVELCHDPPQI----QLLAQIKELT-----------NHVQRLSEALELSESELKALAYNQLRLEEEIQVKTNSLYIDEVICHQLRQHITIQSF 402          
BLAST of SMED30027478 vs. Ensembl Zebrafish
Match: tekt3 (tektin 3 [Source:ZFIN;Acc:ZDB-GENE-080130-2])

HSP 1 Score: 256.914 bits (655), Expect = 3.729e-80
Identity = 148/404 (36.63%), Postives = 241/404 (59.65%), Query Frame = -3
Query:   66 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAH-LQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESI 1274
            RY   +W  SN + YR +E  R +AERL+ +  RLI + ++ T ++ +  +K I +R++DI +W++E++ ++ ++  E   L +  +R+ +A      PL ++++C+ +RE R++IDLVHDDV+ +LIKEV+V    +E + R +E+AV QL  NR A++ L+RD+ DK+TAQ ID+    L+  S  I Y      +DP    P+ W  FT++NIL+   +   S  +R  I+ +L  T+++   Q  +V+ +F  RI+E  D+K  L+ HLA+ L+EI   E  I+ L+ AI  K  PLK+AQTRL+ R  RPNVE CRDNP +      I +V  I            +D  H L+ RL +A +  + L + ++ LE ++ +K NSLF+D+ +CM +R+S 
Sbjct:   76 RYMPEDWYKSNQSNYRESESSRNSAERLRRDTIRLIQDKEQLTRRTQDTTSKNIGERLNDISFWRSEINHEIDNMVTEISALSDVKRRLERALAETEGPLQVAQECLFHREKRMSIDLVHDDVEIDLIKEVDVIKSCQERMRRHLERAVAQLAANRAAQHELERDLSDKVTAQRIDDRCHHLRNTSDGISYYRGIDRLDPSISLPDSWSKFTDDNILHSQSERAASHKLRDEIEILLNATSNEMWNQFNSVNVSFTNRISETADTKNNLQTHLAKTLQEIYQTEMLIEALKKAIRDKENPLKVAQTRLEERTRRPNVELCRDNPHH----RLITEVREI------------EDTIHKLRDRLMEAENTLQTLVKTKITLEHDLSIKANSLFLDQEKCMSMRKSF 463          
BLAST of SMED30027478 vs. Ensembl Zebrafish
Match: tekt3 (tektin 3 [Source:ZFIN;Acc:ZDB-GENE-080130-2])

HSP 1 Score: 257.299 bits (656), Expect = 3.821e-80
Identity = 148/404 (36.63%), Postives = 241/404 (59.65%), Query Frame = -3
Query:   66 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAH-LQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESI 1274
            RY   +W  SN + YR +E  R +AERL+ +  RLI + ++ T ++ +  +K I +R++DI +W++E++ ++ ++  E   L +  +R+ +A      PL ++++C+ +RE R++IDLVHDDV+ +LIKEV+V    +E + R +E+AV QL  NR A++ L+RD+ DK+TAQ ID+    L+  S  I Y      +DP    P+ W  FT++NIL+   +   S  +R  I+ +L  T+++   Q  +V+ +F  RI+E  D+K  L+ HLA+ L+EI   E  I+ L+ AI  K  PLK+AQTRL+ R  RPNVE CRDNP +      I +V  I            +D  H L+ RL +A +  + L + ++ LE ++ +K NSLF+D+ +CM +R+S 
Sbjct:   91 RYMPEDWYKSNQSNYRESESSRNSAERLRRDTIRLIQDKEQLTRRTQDTTSKNIGERLNDISFWRSEINHEIDNMVTEISALSDVKRRLERALAETEGPLQVAQECLFHREKRMSIDLVHDDVEIDLIKEVDVIKSCQERMRRHLERAVAQLAANRAAQHELERDLSDKVTAQRIDDRCHHLRNTSDGISYYRGIDRLDPSISLPDSWSKFTDDNILHSQSERAASHKLRDEIEILLNATSNEMWNQFNSVNVSFTNRISETADTKNNLQTHLAKTLQEIYQTEMLIEALKKAIRDKENPLKVAQTRLEERTRRPNVELCRDNPHH----RLITEVREI------------EDTIHKLRDRLMEAENTLQTLVKTKITLEHDLSIKANSLFLDQEKCMSMRKSF 478          
BLAST of SMED30027478 vs. Ensembl Zebrafish
Match: tekt3 (tektin 3 [Source:ZFIN;Acc:ZDB-GENE-080130-2])

HSP 1 Score: 257.299 bits (656), Expect = 3.821e-80
Identity = 148/404 (36.63%), Postives = 241/404 (59.65%), Query Frame = -3
Query:   66 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAH-LQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESI 1274
            RY   +W  SN + YR +E  R +AERL+ +  RLI + ++ T ++ +  +K I +R++DI +W++E++ ++ ++  E   L +  +R+ +A      PL ++++C+ +RE R++IDLVHDDV+ +LIKEV+V    +E + R +E+AV QL  NR A++ L+RD+ DK+TAQ ID+    L+  S  I Y      +DP    P+ W  FT++NIL+   +   S  +R  I+ +L  T+++   Q  +V+ +F  RI+E  D+K  L+ HLA+ L+EI   E  I+ L+ AI  K  PLK+AQTRL+ R  RPNVE CRDNP +      I +V  I            +D  H L+ RL +A +  + L + ++ LE ++ +K NSLF+D+ +CM +R+S 
Sbjct:   91 RYMPEDWYKSNQSNYRESESSRNSAERLRRDTIRLIQDKEQLTRRTQDTTSKNIGERLNDISFWRSEINHEIDNMVTEISALSDVKRRLERALAETEGPLQVAQECLFHREKRMSIDLVHDDVEIDLIKEVDVIKSCQERMRRHLERAVAQLAANRAAQHELERDLSDKVTAQRIDDRCHHLRNTSDGISYYRGIDRLDPSISLPDSWSKFTDDNILHSQSERAASHKLRDEIEILLNATSNEMWNQFNSVNVSFTNRISETADTKNNLQTHLAKTLQEIYQTEMLIEALKKAIRDKENPLKVAQTRLEERTRRPNVELCRDNPHH----RLITEVREI------------EDTIHKLRDRLMEAENTLQTLVKTKITLEHDLSIKANSLFLDQEKCMSMRKSF 478          
BLAST of SMED30027478 vs. Ensembl Zebrafish
Match: tekt4 (tektin 4 [Source:ZFIN;Acc:ZDB-GENE-070410-98])

HSP 1 Score: 178.718 bits (452), Expect = 1.389e-50
Identity = 129/400 (32.25%), Postives = 214/400 (53.50%), Query Frame = -3
Query:   75 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIR 1274
            +YT  EW  +N+     A  +R  AE + +E   L  ETD  T+++  +    +  R+ DI  W++EL++ +  ++ E        +R+ KA +A   P  I+   +  RE R   DLV D V++EL+KEVE+   ++ LL +T++Q + Q+RLNR+ ++ L+ D  DK  A  +D+        S D     ++A +  +    E W+ FT +N+   +R+   S  +R + + VLQ+T +D + Q  AVD AF +R  E+  +K +LE HLA++L++I   E  I  L+ A+  K  PL++AQ+RL  R  RPN+E CRD PQ     S + +   ++             ++ LQ++L +A      L+  +L+LE++I  K NSL ID  +CM  R
Sbjct:   55 KYTPDEWFDNNSALLNRAAADRNQAENICHESRALKAETDAATLRTQTEGTTHLGARLQDIHLWRSELERLIERLSAETDLQISSRRRLEKALDATEIPFAIATDNLTCRERRFGPDLVKDAVEEELLKEVELIRSIQTLLKKTLDQTINQIRLNREVKHTLELDWSDKQQAYSLDDHCGRFSNRSTDTQRHPSSAVLQDQVCDEEVWRKFTVDNLRLAEREETASQALRRLTERVLQETTEDLRAQCDAVDRAFTQRCVEMSQAKTQLELHLAQILEQIGAQERNISQLQEAVHDKEAPLRVAQSRLHYRTLRPNMEQCRDQPQL----SLLGEASKLS-----------NTVSALQQQLYEAQKSLSDLEESRLNLEKDIACKTNSLLIDREKCMTHR 439          
BLAST of SMED30027478 vs. Ensembl Xenopus
Match: gdf10 (growth differentiation factor 10 [Source:Xenbase;Acc:XB-GENE-5998698])

HSP 1 Score: 349.747 bits (896), Expect = 4.854e-117
Identity = 196/417 (47.00%), Postives = 266/417 (63.79%), Query Frame = -3
Query:   51 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKIEAY 1301
            MA LV+ P ++   EW  +N T Y  AE +R  +ERL  E  RL+DE +K T  S  DVNKK+DQR+++I++WK ELD KL D   +  +L  +  R+ KA E+  EPL I++QC+  RE RV IDLVHD+ ++ELIKEVEV  GV  LL+RTIEQ  EQ+R++R A+Y L++D+ DK  A  ID     L  NS DI Y+     I+  +VTP +W+++TN NIL  ++Q  N+  +RS+IDG+L +TA D  +Q      A + RI E +D+K KLEEHLA+V+ EIS+ E  I+ L+ A+  K  P+K+AQTRL  R  RPNVE CRD  QY      I +V  IT            ++  L+  LA A +E KAL R QL LEEEIQVK+NS++IDEV CM +RE+I I A+
Sbjct:    1 MAKLVQLPPKFLPKEWNIANKTCYARAESQRSRSERLVAESQRLVDEIEKTTQNSQKDVNKKLDQRLEEIQFWKKELDSKLEDTVNDIAQLLAYKIRLEKALESCKEPLSIAQQCLIEREKRVGIDLVHDEAERELIKEVEVIQGVMTLLERTIEQITEQIRMSRSAKYSLEKDLKDKFQALKIDNHCAGLTNNSPDIDYSANTVRIEANSVTPIEWENYTNTNILKAEKQRNNAIALRSLIDGILSETASDICKQCDTATYALKNRIQETKDAKNKLEEHLAKVMDEISSQEKNIEILKKALADKEGPMKMAQTRLDTRTLRPNVELCRDPVQY----RLINEVQEITT-----------NVERLRETLAQAEAELKALNRNQLSLEEEIQVKENSIYIDEVICMQMREAISINAF 402          
BLAST of SMED30027478 vs. Ensembl Xenopus
Match: tekt3 (tektin 3 [Source:NCBI gene;Acc:549229])

HSP 1 Score: 256.144 bits (653), Expect = 1.547e-79
Identity = 152/403 (37.72%), Postives = 235/403 (58.31%), Query Frame = -3
Query:   66 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESI 1274
            RYT  +W  SN   YR++E  R++AERL+ +  RLI E D++T K  +D NK + +R++DI +WK+E+  ++  +  E   L E  +R+ +A      PL +S++C+ +RE R++IDLVHDDV+K+LI+EV+     +E + R +++   QL  NR A++ L+RD+ DK TA  ID+  + L+ +S  IGY      +D     PE W  ++++NIL      + S+ +R   + +L  T++D   Q  AV+  F  RI+E  D+K KL+ HLA+ L+EI   E TI+ ++ AI+ K  PLK+AQTRL  R  RPNVE CRD+ Q       +  +DN               I  LQ RL +A    + L   +  LE ++ +K NSLFID+ +CMG+R+S 
Sbjct:   93 RYTPDDWYKSNQVNYRDSESSRRSAERLRVDTSRLIQEKDQKTRKVQSDTNKDLGERVNDISFWKSEIIHEIDQMIGETNALSEMKRRMERALMETEGPLQVSQECLYHREKRMSIDLVHDDVEKQLIQEVDCIKSCQEKMRRHLQKISAQLASNRAAQHELERDISDKQTALRIDDKCQNLRNSSDGIGYFRGVERMDATVSIPETWAKYSDDNILRSQSDRVASTKLREDAETILASTSNDMWGQFNAVNVNFTSRISETADAKNKLQNHLAKTLQEIFQTEMTIEAIKKAIKDKSAPLKVAQTRLDERTRRPNVELCRDSVQM-RLIQEVQDIDNT--------------IQALQLRLREAEDALQMLIHTKSILEHDLAIKANSLFIDQEKCMGMRKSF 480          
BLAST of SMED30027478 vs. Ensembl Xenopus
Match: plcd1 (phospholipase C delta 1 [Source:Xenbase;Acc:XB-GENE-1013054])

HSP 1 Score: 223.787 bits (569), Expect = 2.162e-67
Identity = 140/400 (35.00%), Postives = 221/400 (55.25%), Query Frame = -3
Query:   75 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIR 1274
            +Y H EW   N   +  A  +R  +E  ++E  +L+ ET+ R  +S  D  K + +R+ DI +WK+EL +++ DV  E   L +   R+ +A +A   P  I+   +  R+ R   +LV D+V+ EL+KEV++   V+ELL RT++Q V+Q+R NR A+  L+ D  DK+ A   D+        S DI +   +++ +    TPE W  +T+ENI   +R+ + S ++RS+ID +LQ  ++D + Q  A+   F +R  EV D+K KLE HL + L+EI   E  I  L+ AI  K  PLK+AQTRL  R  RPNVE CRD+ Q       + +V  +T             +  L+ +L D+    + L+  ++ LE++I  K NS+FID  +CM +R
Sbjct:   63 KYLHEEWQQGNLATFYRAFSDRDQSETGRHEAKQLVTETEARAQRSQADCTKSLGERLLDIHFWKSELAREIRDVEAETQLLVQQKGRLERALDATDIPFSIATDNLKCRDRRRGSELVRDEVELELLKEVDLIRNVQELLKRTLDQTVQQIRQNRDAKENLEMDYSDKVEAYEFDDKCGRYNNQSTDIQFHRNSSKYEDNTSTPESWAQYTHENIYKAERERMASINLRSLIDSILQDISEDLEAQSAAIAVDFEKRCREVEDAKQKLEMHLKKTLEEIGGQEKNIAALKQAINDKSPPLKVAQTRLHDRSYRPNVELCRDHVQ----IRLVSEVGELTD-----------SLDALKLKLEDSEQSLRNLEDTRMSLEKDIANKANSIFIDREKCMTLR 447          
BLAST of SMED30027478 vs. Ensembl Xenopus
Match: fam168a (family with sequence similarity 168 member A [Source:Xenbase;Acc:XB-GENE-5856038])

HSP 1 Score: 194.126 bits (492), Expect = 8.942e-58
Identity = 121/292 (41.44%), Postives = 170/292 (58.22%), Query Frame = -3
Query:  321 RLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVE 1196
            RL  E  RL+DE++K T KS  D NKK+DQR+++I++WK ELD KL D   +  +L     R+ K+ E   EPL +++QC+  +E RV IDLVHD+ ++ELIK V+V  GV  LL+          R N K  Y L++D+ DK  A  ID     L  NS DIGY+     I+  +VTP +W+ FTN NI+  +++  N+  +RS+IDG+L +TA D                     +K KLEEHLA+V+ EIS+ E  I+ L+ A+  K  P+K+ Q RL  R  RPNVE
Sbjct:   18 RLVAESQRLVDESEKTTQKSQKDFNKKLDQRLEEIQFWK-ELDSKLEDTVNDIAQLLACKFRLEKSLERCKEPLSLAQQCLIKQEKRVGIDLVHDEAEQELIKAVDVIQGVMTLLEYN--------RTNSK--YSLEKDLKDKFQALKIDNHCAGLTNNSPDIGYSANTVRIEANSVTPIEWEDFTNTNIVKAEKRRNNAVALRSLIDGILSETASDIY-------------------AKNKLEEHLAKVISEISSQEKNIEILKKALADKEGPMKMEQIRLDTRTLRPNVE 279          
BLAST of SMED30027478 vs. Ensembl Xenopus
Match: fam168a (family with sequence similarity 168 member A [Source:Xenbase;Acc:XB-GENE-5856038])

HSP 1 Score: 182.185 bits (461), Expect = 1.785e-53
Identity = 111/276 (40.22%), Postives = 160/276 (57.97%), Query Frame = -3
Query:  321 TVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVE 1148
            T KS  D NKK+DQR+++I++WK ELD KL D   +  +L     R+ K+ E   EPL +++QC+  +E RV IDLVHD+ ++ELIK V+V  GV  LL+ ++            A+Y L++D+ DK  A  ID     L  NS DIGY+     I+  +VTP +W+ FTN NI+  +++  N+  +RS+IDG+L +TA D                     +K KLEEHLA+V+ EIS+ E  I+ L+ A+  K  P+K+ Q RL  R  RPNVE
Sbjct:    1 TQKSQKDFNKKLDQRLEEIQFWK-ELDSKLEDTVNDIAQLLACKFRLEKSLERCKEPLSLAQQCLIKQEKRVGIDLVHDEAEQELIKAVDVIQGVMTLLEMSLS-----------AKYSLEKDLKDKFQALKIDNHCAGLTNNSPDIGYSANTVRIEANSVTPIEWEDFTNTNIVKAEKRRNNAVALRSLIDGILSETASDIY-------------------AKNKLEEHLAKVISEISSQEKNIEILKKALADKEGPMKMEQIRLDTRTLRPNVE 245          
BLAST of SMED30027478 vs. Ensembl Mouse
Match: Tekt1 (tektin 1 [Source:MGI Symbol;Acc:MGI:1333819])

HSP 1 Score: 328.946 bits (842), Expect = 7.821e-109
Identity = 177/412 (42.96%), Postives = 268/412 (65.05%), Query Frame = -3
Query:   66 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESI 1301
            MA L++ P R+   EW  +N ++Y  AE +R  +ERL  E  RL++E +K T KS +DVNKK++QR++++++WK ELD KL  +  +   L  +  R+ ++ E+  EPLHI+++C+  RE RV IDLVHD V++EL KE ++ +GV  LL RT+E++ EQ+RLNR A+Y L++D+ DK TA  ID+    L  NS +I ++     I+P +V+ EDW  F+N N+   D+QL NS+ +++++D +L QTA+D +RQ   VD AF   + E +D++ KL +HLA+V++EI++ E  I  LE AI  +  P K+A TRL+ R  RPNVE CRD  QY      I ++  I             ++A L+  LA A ++ KAL RRQL L+EEIQVK+N+++ID+V CM +R+SI
Sbjct:    1 MAKLLQSPPRFLPEEWYIANKSQYHRAEAQRSQSERLVAESQRLVEEIEKTTRKSQSDVNKKLEQRLEEVRFWKKELDDKLEQLVNQTDDLLTYKTRLERSLESYKEPLHITEKCLEYREKRVGIDLVHDVVEQELQKEADIIHGVMNLLIRTLEESTEQIRLNRSAKYNLEKDLRDKFTAITIDDVCFSLNNNSPNINFSEKVVRIEPNSVSLEDWLDFSNANVEKADKQLNNSTALKTLVDQILSQTANDLRRQCEVVDEAFINGLKETKDARNKLADHLAKVMEEIASQEKNIMALENAITQQEGPAKVAHTRLETRTHRPNVELCRDIAQY----RLIKEIQEIN-----------HNVARLKETLAQAQTQLKALYRRQLALQEEIQVKENTIYIDQVLCMEMRKSI 397          
BLAST of SMED30027478 vs. Ensembl Mouse
Match: Tekt1 (tektin 1 [Source:MGI Symbol;Acc:MGI:1333819])

HSP 1 Score: 328.946 bits (842), Expect = 7.821e-109
Identity = 177/412 (42.96%), Postives = 268/412 (65.05%), Query Frame = -3
Query:   66 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESI 1301
            MA L++ P R+   EW  +N ++Y  AE +R  +ERL  E  RL++E +K T KS +DVNKK++QR++++++WK ELD KL  +  +   L  +  R+ ++ E+  EPLHI+++C+  RE RV IDLVHD V++EL KE ++ +GV  LL RT+E++ EQ+RLNR A+Y L++D+ DK TA  ID+    L  NS +I ++     I+P +V+ EDW  F+N N+   D+QL NS+ +++++D +L QTA+D +RQ   VD AF   + E +D++ KL +HLA+V++EI++ E  I  LE AI  +  P K+A TRL+ R  RPNVE CRD  QY      I ++  I             ++A L+  LA A ++ KAL RRQL L+EEIQVK+N+++ID+V CM +R+SI
Sbjct:    1 MAKLLQSPPRFLPEEWYIANKSQYHRAEAQRSQSERLVAESQRLVEEIEKTTRKSQSDVNKKLEQRLEEVRFWKKELDDKLEQLVNQTDDLLTYKTRLERSLESYKEPLHITEKCLEYREKRVGIDLVHDVVEQELQKEADIIHGVMNLLIRTLEESTEQIRLNRSAKYNLEKDLRDKFTAITIDDVCFSLNNNSPNINFSEKVVRIEPNSVSLEDWLDFSNANVEKADKQLNNSTALKTLVDQILSQTANDLRRQCEVVDEAFINGLKETKDARNKLADHLAKVMEEIASQEKNIMALENAITQQEGPAKVAHTRLETRTHRPNVELCRDIAQY----RLIKEIQEIN-----------HNVARLKETLAQAQTQLKALYRRQLALQEEIQVKENTIYIDQVLCMEMRKSI 397          
BLAST of SMED30027478 vs. Ensembl Mouse
Match: Tekt1 (tektin 1 [Source:MGI Symbol;Acc:MGI:1333819])

HSP 1 Score: 328.946 bits (842), Expect = 7.821e-109
Identity = 177/412 (42.96%), Postives = 268/412 (65.05%), Query Frame = -3
Query:   66 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESI 1301
            MA L++ P R+   EW  +N ++Y  AE +R  +ERL  E  RL++E +K T KS +DVNKK++QR++++++WK ELD KL  +  +   L  +  R+ ++ E+  EPLHI+++C+  RE RV IDLVHD V++EL KE ++ +GV  LL RT+E++ EQ+RLNR A+Y L++D+ DK TA  ID+    L  NS +I ++     I+P +V+ EDW  F+N N+   D+QL NS+ +++++D +L QTA+D +RQ   VD AF   + E +D++ KL +HLA+V++EI++ E  I  LE AI  +  P K+A TRL+ R  RPNVE CRD  QY      I ++  I             ++A L+  LA A ++ KAL RRQL L+EEIQVK+N+++ID+V CM +R+SI
Sbjct:    1 MAKLLQSPPRFLPEEWYIANKSQYHRAEAQRSQSERLVAESQRLVEEIEKTTRKSQSDVNKKLEQRLEEVRFWKKELDDKLEQLVNQTDDLLTYKTRLERSLESYKEPLHITEKCLEYREKRVGIDLVHDVVEQELQKEADIIHGVMNLLIRTLEESTEQIRLNRSAKYNLEKDLRDKFTAITIDDVCFSLNNNSPNINFSEKVVRIEPNSVSLEDWLDFSNANVEKADKQLNNSTALKTLVDQILSQTANDLRRQCEVVDEAFINGLKETKDARNKLADHLAKVMEEIASQEKNIMALENAITQQEGPAKVAHTRLETRTHRPNVELCRDIAQY----RLIKEIQEIN-----------HNVARLKETLAQAQTQLKALYRRQLALQEEIQVKENTIYIDQVLCMEMRKSI 397          
BLAST of SMED30027478 vs. Ensembl Mouse
Match: Tekt5 (tektin 5 [Source:MGI Symbol;Acc:MGI:1917676])

HSP 1 Score: 221.476 bits (563), Expect = 8.687e-66
Identity = 139/403 (34.49%), Postives = 221/403 (54.84%), Query Frame = -3
Query:   66 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESI 1274
            RYT  +W  SN+ + RNAE  R  A RL  +  R++ + D+   +     ++ + QR+ D+ +WK+EL  +L  +  EN  +D   +R+  A E +  PL ++ +C+ NRE R+ IDLVHD+V+K LI+EV++    ++ + +  ++   Q+R NR A++ L+RD++DK +AQ IDE    L+  S  I + +   + D     PE W  F+N+NI +      NS  +R   + + +  +D   +Q    + AF  RI+E  D K KL+  LA++L+EI   E+TI  LE AI AK  PLK+AQT L  R  RPNVE CRD PQ+      + +V  I           +  +  L+ RL +     + L   +  LE E+ +K N+L ID+ +CM +R+S 
Sbjct:   89 RYTPRDWDRSNDLQIRNAEASRLWASRLTGDSLRIMQDKDQLIHQMQEGTSRNLGQRLSDLGFWKSELCYELDRLLTENSSMDTLKRRLECAAEEVNCPLQVALECLYNREKRIGIDLVHDNVEKNLIREVDLLKCCQDQMRKLAKRIDFQIRDNRDAQHSLERDIEDKSSAQYIDENCFNLRSTSDSISFFHGVEKFDGTVSIPETWAKFSNDNIRHAQNMRANSIRLREEAEHLFETLSDQMWKQFTNTNLAFNARISEETDVKNKLQTQLAKILQEIFQAENTIMLLERAIVAKEYPLKMAQTMLACRTRRPNVELCRDVPQF----RLVNEVFTI-----------DDTLQTLKLRLRETQDTLQLLVMTKSRLEHELAIKANTLCIDKDKCMSMRKSF 476          
BLAST of SMED30027478 vs. Ensembl Mouse
Match: Tekt4 (tektin 4 [Source:MGI Symbol;Acc:MGI:1919090])

HSP 1 Score: 216.468 bits (550), Expect = 7.084e-65
Identity = 136/400 (34.00%), Postives = 225/400 (56.25%), Query Frame = -3
Query:   75 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIR 1274
            +Y   EW  ++  +Y  A  +R  +ER ++E  +L+ ET     ++  D  +K+ +R++D+  WK+EL +++ +++ E   +     R+ +A +A   P  I+   +  RE R   DLV D V+ EL+KE E+   ++ELL RTI QAV+Q+RLNR+ +   + +  DK+    ID+        S  + +   +++ +  A TPE W  F ++N+L  +R+ L S ++R +ID +L+ TA+D + Q  AV++AF  R  E+ DS  KL+ HL + L EI++ E  I  L+ AI+ K  PL++AQTRL  R  RPNVE CRDN Q+      + +V+ + +            +  L+ +L DA    + L+  ++ LE++I VK NSLFID  +CM  R
Sbjct:   50 KYLRDEWFQNSYARYHQAFADRDYSERQRHESGQLVAETGALAQRTQLDSTRKVGERLEDMHCWKSELQREIDELSSETDLMMAQKLRLQRALDATSVPYSIATDNLQCRERRQHPDLVRDYVEVELLKETELIRNIQELLKRTIGQAVDQIRLNREHKESCEMNWSDKVEVYNIDDTCSRYTNESTQVQFYPHSSKFEESASTPETWAKFNHDNLLRAERERLASVNLRKLIDCILRDTAEDLRLQCDAVNSAFSSRCQELDDSLQKLQYHLRKTLTEITDQEHQIAALKQAIKDKEAPLRVAQTRLYQRSHRPNVELCRDNAQF----RLLSEVEELNM-----------SLRALKEKLQDAEQALRNLEDSRMSLEKDIAVKTNSLFIDRQKCMTHR 434          
BLAST of SMED30027478 vs. UniProt/SwissProt
Match: sp|Q99JD2|TEKT1_RAT (Tektin-1 OS=Rattus norvegicus OX=10116 GN=Tekt1 PE=2 SV=1)

HSP 1 Score: 333.569 bits (854), Expect = 9.849e-110
Identity = 184/412 (44.66%), Postives = 268/412 (65.05%), Query Frame = -3
Query:   66 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESI 1301
            MA L++ P ++   EW  +N ++Y  AE +R  +ERL  E  RL++E +K T KS +DVNKK++QR++++K+WK ELD KL  +  E   L  +  R+ ++ E+  EPLHI+++C+A RE RV IDLVHD V +EL KE ++ +GV  LL RT+E+A EQ+RLNR A+Y L++D+ DK TA  ID+    L  NS DI Y+     I+P +V+ EDW  F+N N+   D+QL NS  +++++D +L QTA+D +RQ   VDTAF   + E +D++ KL +HLA+V+ EI++ E  I+ LE AI  +  P K+A TRL+ R  RPNVE CRD  QY      I +V  I           + ++A L+  LA A  + KAL RRQL L+EEIQVK+N+++ID+V CM +R+SI
Sbjct:    1 MAKLLQSPPKFLPAEWYIANKSQYHRAEAQRSRSERLVAESQRLVEEIEKTTRKSQSDVNKKLEQRLEEVKFWKKELDDKLEHLVNETEDLLTYKIRLERSLESYKEPLHITEKCLAYREKRVGIDLVHDVVDQELQKEADIIHGVMNLLTRTVEEATEQIRLNRSAKYNLEKDLKDKFTAITIDDICFSLNNNSPDIKYSENVVRIEPNSVSLEDWLDFSNTNMQKADKQLNNSMALKTLVDQILSQTANDLRRQCEVVDTAFVNGLKETKDARNKLADHLAKVMDEIASQEKNIEVLENAITQQEGPAKVAHTRLENRTYRPNVELCRDVAQY----RLIREVQEI-----------KHNVARLEETLAQAQIQLKALFRRQLALQEEIQVKENTIYIDQVLCMEMRKSI 397          
BLAST of SMED30027478 vs. UniProt/SwissProt
Match: sp|Q969V4|TEKT1_HUMAN (Tektin-1 OS=Homo sapiens OX=9606 GN=TEKT1 PE=1 SV=1)

HSP 1 Score: 333.183 bits (853), Expect = 1.171e-109
Identity = 181/414 (43.72%), Postives = 267/414 (64.49%), Query Frame = -3
Query:   60 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKI 1301
            MA L++PP ++   EW  +N  +Y  A+ +R  +ERL  E  RL+DE +K T KS +DVNKK++QR++++++WK ELD KL  +      L  +  R+ KA E L EPLHI++ C+A RE R+ IDLVHD V+ ELIKE E+  G+  LL RT+E+A EQ+R+NR A+Y L++D+ DK  A  ID+    L  NS +I Y+  A  I+P +V+ EDW  F++ N+   D+Q  NS  +++++D +L QTA+D ++Q   VDTAF+  + + +D++ KL +HLA+V++EI++ E  I  LE AI  +  P K+A TRL+ R  RPNVE CRD  QY      + +V  IT            ++A L+  LA A +E K L RRQL L+EEIQVK+N+++IDEV CM +R+SI +
Sbjct:    1 MAKLLQPPPKFLPSEWHIANKNQYHRADAQRSRSERLVAESQRLVDEIEKTTRKSQSDVNKKLEQRLEEVQFWKKELDDKLEQLVNVTDDLLIYKIRLEKALETLKEPLHITETCLAYREKRIGIDLVHDTVEHELIKEAEIIQGIMALLTRTLEEASEQIRMNRSAKYNLEKDLKDKFVALTIDDICFSLNNNSPNIRYSENAVRIEPNSVSLEDWLDFSSTNVEKADKQRNNSLMLKALVDRILSQTANDLRKQCDVVDTAFKNGLKDTKDARDKLADHLAKVMEEIASQEKNITALEKAILDQEGPAKVAHTRLETRTHRPNVELCRDVAQY----RLMKEVQEIT-----------HNVARLKETLAQAQAELKGLHRRQLALQEEIQVKENTIYIDEVLCMQMRKSIPL 399          
BLAST of SMED30027478 vs. UniProt/SwissProt
Match: sp|Q32KZ9|TEKT1_BOVIN (Tektin-1 OS=Bos taurus OX=9913 GN=TEKT1 PE=2 SV=1)

HSP 1 Score: 331.643 bits (849), Expect = 5.451e-109
Identity = 182/412 (44.17%), Postives = 264/412 (64.08%), Query Frame = -3
Query:   66 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESI 1301
            MA L++ P ++   EW  +N  +Y  AE +R  +ERL  E  RL+DE +K T KS +DVNKK++QR++++++WK ELD KL  +      L  +  R+ KA E+  EPLHI+++C+  RE RV IDLVHD+V++ELIKE E+  GV  LL RT+E+  EQ+RLNR A+Y L++D+ DK TA  ID+    L  NS +I Y+     ++P +V+ EDW  F+N N+   D+Q  NS  +++++D +L QTA D +RQ   VDTAF+  + E +D++ KL  HL +V++EI++ E  I  LE AI  +  P K+A TRL+ R  RPNVE CRD  QY      I +VD IT            ++A L+  LA A+ E K L RRQL L+EEIQ+K+N+++IDEV C+ +R+SI
Sbjct:    1 MAKLLQQPPKFLRAEWQIANKNQYHRAEAQRSRSERLVAESQRLVDEIEKTTRKSQSDVNKKLEQRLEEVRFWKKELDDKLEQLVYATEDLLLYQTRLQKALESFKEPLHITEKCLEYREKRVGIDLVHDEVEQELIKEHEIIRGVMTLLTRTLEETCEQIRLNRSAKYNLEKDLRDKFTAITIDDICFSLNNNSPNIKYSENVVRVEPNSVSLEDWLDFSNTNVEKADKQRNNSLTLKALVDRILFQTASDLRRQCDVVDTAFKNGLKETKDARDKLALHLDKVMEEIASQEKNIVVLEKAILDQEGPAKVAHTRLETRTHRPNVELCRDVAQY----RLIKEVDEIT-----------HNVARLKETLAQAHVELKGLNRRQLALQEEIQIKENTIYIDEVLCVPMRKSI 397          
BLAST of SMED30027478 vs. UniProt/SwissProt
Match: sp|Q9DAJ2|TEKT1_MOUSE (Tektin-1 OS=Mus musculus OX=10090 GN=Tekt1 PE=1 SV=1)

HSP 1 Score: 328.946 bits (842), Expect = 5.470e-108
Identity = 177/412 (42.96%), Postives = 268/412 (65.05%), Query Frame = -3
Query:   66 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESI 1301
            MA L++ P R+   EW  +N ++Y  AE +R  +ERL  E  RL++E +K T KS +DVNKK++QR++++++WK ELD KL  +  +   L  +  R+ ++ E+  EPLHI+++C+  RE RV IDLVHD V++EL KE ++ +GV  LL RT+E++ EQ+RLNR A+Y L++D+ DK TA  ID+    L  NS +I ++     I+P +V+ EDW  F+N N+   D+QL NS+ +++++D +L QTA+D +RQ   VD AF   + E +D++ KL +HLA+V++EI++ E  I  LE AI  +  P K+A TRL+ R  RPNVE CRD  QY      I ++  I             ++A L+  LA A ++ KAL RRQL L+EEIQVK+N+++ID+V CM +R+SI
Sbjct:    1 MAKLLQSPPRFLPEEWYIANKSQYHRAEAQRSQSERLVAESQRLVEEIEKTTRKSQSDVNKKLEQRLEEVRFWKKELDDKLEQLVNQTDDLLTYKTRLERSLESYKEPLHITEKCLEYREKRVGIDLVHDVVEQELQKEADIIHGVMNLLIRTLEESTEQIRLNRSAKYNLEKDLRDKFTAITIDDVCFSLNNNSPNINFSEKVVRIEPNSVSLEDWLDFSNANVEKADKQLNNSTALKTLVDQILSQTANDLRRQCEVVDEAFINGLKETKDARNKLADHLAKVMEEIASQEKNIMALENAITQQEGPAKVAHTRLETRTHRPNVELCRDIAQY----RLIKEIQEIN-----------HNVARLKETLAQAQTQLKALYRRQLALQEEIQVKENTIYIDQVLCMEMRKSI 397          
BLAST of SMED30027478 vs. UniProt/SwissProt
Match: sp|Q9TTW3|TEKT1_CANLF (Tektin-1 OS=Canis lupus familiaris OX=9615 GN=TEKT1 PE=2 SV=1)

HSP 1 Score: 317.39 bits (812), Expect = 2.478e-103
Identity = 171/411 (41.61%), Postives = 258/411 (62.77%), Query Frame = -3
Query:   69 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRES 1301
            MA L++P  ++   EW  +N  +Y  AE +R  +ERL  E  RL+DE +K T KS +DVNKK++QR++++++WK ELD KL  +      L  +  R+  A E+L EPLHI++ C+  R+ R+ IDLVHD+V++EL+KE EV +GV  LL RT+++  EQ+RLNR A+Y L++D+ DK  A  ID+    L  NS  I Y+++   ++P +V+ EDW  F+N N+   +RQ  NS  +++++D +L QTADD +RQ   VDTAF+  + E + ++ +L  HLA+V++EI+  E  +  LE AI  +  P K+A TRL+ R  RPNVE CRD  QY      + +V  I            +++  L+  LA A  E K L RRQL L+EE+QVK+N+++ID V C  +R+S
Sbjct:    1 MAKLLQPSPKFLPEEWHIANKNQYHRAEAQRSRSERLVAESQRLVDEIEKTTRKSQSDVNKKLEQRLEEVRFWKKELDDKLEQLVCTTEDLLTYQTRLQNALESLKEPLHITQMCLEYRDKRIGIDLVHDEVEQELLKEAEVIHGVMALLTRTMDEVTEQIRLNRSAKYNLEKDLKDKFVALTIDDVCFSLNNNSPGIYYSDSVVRVEPHSVSLEDWLDFSNTNVEKANRQRNNSLALKALVDRILSQTADDLRRQCDMVDTAFQMGLKETKAARDQLAAHLAKVMEEIACQEKNMTVLEKAILDQEGPAKVAHTRLETRTRRPNVELCRDVAQY----RLVKEVGEIA-----------QNVGRLKEALAQAQVELKGLNRRQLALQEEVQVKENTIYIDRVLCTHMRKS 396          
BLAST of SMED30027478 vs. TrEMBL
Match: A0A4S2M0K2 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_004892 PE=4 SV=1)

HSP 1 Score: 439.884 bits (1130), Expect = 2.906e-149
Identity = 231/417 (55.40%), Postives = 292/417 (70.02%), Query Frame = -3
Query:   51 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKIEAY 1301
            MA L+K P R+TH EWT+SNN KYR+AE ER+ ++ LQNECDR +DE +KRT ++L DV+KKIDQRI DIKYWK+E++KKL DV  E   L     R+ KA EA  EPLH+++QC+  REGR  IDLVHDD QKEL KEVEV+ GV+ LL +T+EQ  EQLRLNRKA Y LK+D  DKL+AQ IDEF   LK   + +    +   IDP + T E+WQ+F++  I   D+QL NS  +RSIIDG+LQQ A DQ+RQ  A + A ++RI E RD+KGKLEEHL+RVL+EIS +ED I  L  +I+ K   L+LA TR+ +RK RPNVE CRD  +Y      I +VD IT            D+A L+ RL  A+   KAL RRQLDLEEEIQ+K  +LFIDEV+CMG+RES++I AY
Sbjct:    1 MAGLLKCPQRFTHEEWTFSNNVKYRSAEKEREFSQGLQNECDRYMDEAEKRTDRTLKDVDKKIDQRITDIKYWKSEVNKKLDDVTGETDALHASFIRLKKALEATEEPLHLAQQCIITREGRSGIDLVHDDAQKELCKEVEVYKGVQALLQKTLEQTKEQLRLNRKAIYNLKKDAGDKLSAQHIDEFALSLKSTDESLPGLKSGTLIDPNSFTTEEWQNFSHNTIQAGDKQLQNSQQLRSIIDGILQQIASDQRRQVEATNRALKKRIAETRDAKGKLEEHLSRVLEEISKMEDMISELNKSIKDKEGALRLAGTRIDLRKVRPNVELCRDAAEY----RLIQEVDEITT-----------DVAELRHRLKLAHDSLKALCRRQLDLEEEIQIKAATLFIDEVQCMGMRESLQINAY 402          
BLAST of SMED30027478 vs. TrEMBL
Match: G4V8P1 (Putative tektin OS=Schistosoma mansoni OX=6183 GN=Smp_165950 PE=4 SV=1)

HSP 1 Score: 435.261 bits (1118), Expect = 2.301e-147
Identity = 224/417 (53.72%), Postives = 293/417 (70.26%), Query Frame = -3
Query:   51 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKIEAY 1301
            MA LV+ P R+TH EWTYSNN KYR+AE ER+ ++ LQNECDRLI+ET KRT K+L DV KK DQRI ++KYWK+E++KKL D+  E   LDE+  R+ K  EA  EPLH ++QC+ +REGR  IDLVHDD Q ELIKE+EV  G + +L RT+EQA EQLRLNRKA Y +K+D  DKL AQ +DE+   LKP  + +  +    +I+P + TPE+WQ+F+  +I N D+QL NS ++RSI+DG++QQ A D KRQ  A + A  +RI+E R SK KLEE+L+ ++KEISN+EDT+  LE AI+ K   L+LA TRL +RK RPNVE CRD   Y      I +V+             ++D+  L++RL  A+   KAL RRQLDLEEEIQ+K  +LFIDEV+CMGIRESIK  ++
Sbjct:    1 MAGLVRQPQRFTHEEWTYSNNLKYRSAEKEREISQGLQNECDRLIEETAKRTEKTLKDVEKKFDQRIANVKYWKSEVNKKLQDITDETEILDEYFIRLRKTLEATEEPLHFAQQCLLSREGRTGIDLVHDDAQMELIKEIEVIKGAQTILQRTVEQAKEQLRLNRKAIYNMKKDSSDKLQAQHLDEYALSLKPTDELLSGSTNVPKIEPNSFTPEEWQTFSTASIENGDKQLQNSHELRSIVDGIIQQVASDIKRQIEATNRALNKRISETRSSKAKLEEYLSAIIKEISNMEDTLHELEKAIKEKDGALRLAGTRLGIRKERPNVELCRDPANY----RLIQEVEE-----------TKRDVEQLRQRLHAAHDSLKALCRRQLDLEEEIQIKAATLFIDEVQCMGIRESIKFSSF 402          
BLAST of SMED30027478 vs. TrEMBL
Match: A0A4Z2DU15 (Tektin-1 isoform 2 OS=Schistosoma japonicum OX=6182 GN=EWB00_005994 PE=4 SV=1)

HSP 1 Score: 431.795 bits (1109), Expect = 5.548e-146
Identity = 228/417 (54.68%), Postives = 290/417 (69.54%), Query Frame = -3
Query:   51 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKIEAY 1301
            MA LV+ P R+TH EWTYSNN KYR+AE ER+ ++ LQNECDR I+ET KRT K+L DV KK DQRI +IKYWK+E++KKL D+  E   LDE+  R+ K  EA  EPLH ++QC+ +RE R  IDLVHDD QKELIKE+E+  G + +L RTIEQA EQLRLNRK  Y LK+D  DKL AQ +DE+   LKP    +  +    +I P + TPE+WQ+F+  +I N DRQL NS ++RSIIDGV+QQ A D KRQ  A + A  +RI+E R SK KLEEHL+ V+KEIS++EDT+  LE AI+ K   L+LA TRL +R+ RPNVE CRD   +      I +V+ I           ++DI  L++RL  A++  KAL RRQLDLEEEIQ+K  +LFIDEV+CMGIR SIK  A+
Sbjct:    1 MAGLVQQPQRFTHEEWTYSNNLKYRSAEKEREISQGLQNECDRFIEETAKRTEKTLKDVEKKFDQRIANIKYWKSEVNKKLQDITEETEILDEYFVRLRKTLEATEEPLHFAQQCLLSRERRTGIDLVHDDAQKELIKEIEIIKGAQAILQRTIEQAKEQLRLNRKVIYNLKKDSSDKLQAQQLDEYALSLKPTDDSLSGSTQLPKIVPHSFTPEEWQTFSINSIENGDRQLQNSHELRSIIDGVIQQVAADIKRQIEATNRALNKRISETRSSKAKLEEHLSAVIKEISDIEDTLHQLEKAIKDKDGILRLAGTRLGIRRERPNVELCRDPADF----RLIQEVEEI-----------KRDIEQLKQRLHAAHASLKALLRRQLDLEEEIQIKAATLFIDEVQCMGIRGSIKFTAF 402          
BLAST of SMED30027478 vs. TrEMBL
Match: A0A183K5D9 (Uncharacterized protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS10212 PE=4 SV=1)

HSP 1 Score: 431.024 bits (1107), Expect = 1.010e-145
Identity = 224/418 (53.59%), Postives = 293/418 (70.10%), Query Frame = -3
Query:   51 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSK-DIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKIEAY 1301
            MA LV+ P R+TH EWTYSNN KYR+AE ER+ ++ LQNECDRLI+ET KRT K+L DV KK D RI ++KYWK+E++KKL D + E   LDE+  R+ K  EA  EPLH ++QC+ +REGR  IDLVHDD Q ELIKE+EV  G + +L +T+EQ  EQLRLNRK  Y +K+D  DKL AQ +DE+   LKP  +   G+TN   +I+P + TPE+WQ+F+  ++ + DRQL NS ++RSIIDG++QQ A D KRQ    + A  +RI+E R SK KLEEHL+ ++KEISN+EDT+  LE AI+ K   L LA+TRL +RK RPNVE CRD   Y      I +V+ I           ++D+  L++RL  A++  KAL RRQLDLEEEIQ+K  +LFIDEV+CMGIRESIK  ++
Sbjct:    1 MAGLVRQPQRFTHEEWTYSNNLKYRSAEKEREISQGLQNECDRLIEETAKRTEKTLKDVEKKFDLRIANVKYWKSEVNKKLQDTSEETEILDEYFIRLKKTLEATEEPLHFAQQCLLSREGRTGIDLVHDDAQMELIKEIEVIKGAQAILQKTVEQTKEQLRLNRKVIYNMKKDSSDKLQAQHLDEYALSLKPTDELPSGFTN-VPKIEPNSFTPEEWQTFSTASVESGDRQLQNSHELRSIIDGIIQQVASDIKRQIETTNRALNKRISETRSSKAKLEEHLSAIIKEISNIEDTLHELEKAIQDKDGALHLAETRLGIRKERPNVELCRDPANY----RLIQEVEEI-----------KRDMEQLRQRLHAAHASLKALCRRQLDLEEEIQIKAATLFIDEVQCMGIRESIKFSSF 402          
BLAST of SMED30027478 vs. TrEMBL
Match: A0A1I8GZC7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 430.254 bits (1105), Expect = 1.860e-145
Identity = 223/417 (53.48%), Postives = 296/417 (70.98%), Query Frame = -3
Query:   51 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKIEAY 1301
            MA +V PP RYTHPEWT+SNN KYRNAE+ER  AERL+ E DR+I+ET K+T  +  DV+KK++QR++D+KYW+ E+D+KLA V+ E  +L   I RV KA E+  EPLHIS+QC+ NREGR+ IDLVHDDVQKEL+KEVEV+ G + LL +T+EQA EQLRLNRKA+Y+L++D+ DK +A+ +D     LK NS  +G +  A  ID K+V+PE+WQ  +N NIL  ++Q  NS+ M SI+DG+LQ  A+D ++QK  VD A +RR+ E RDSK KLEEHLA+VL E+ ++E  I  LE AI  K QPLK+A+TRL+VR  RPNVE CRD  Q+      + +V  I              +  L +RLA +    K L RRQLDLEEEIQ+K N+L+IDEV+C G+R SI+I ++
Sbjct:    1 MAKVVNPPPRYTHPEWTFSNNQKYRNAELERAAAERLKAESDRIIEETAKKTAAAQADVDKKLNQRLNDVKYWREEVDEKLAGVSGEIDRLGAFISRVDKALESYQEPLHISQQCLINREGRIGIDLVHDDVQKELLKEVEVNQGAQALLLKTLEQAKEQLRLNRKAKYQLEKDIADKRSAEQLDSHCVALKANSAGLGRSPCAVGIDSKSVSPEEWQELSNRNILEAEKQRQNSAHMCSIVDGILQAVANDLEKQKSCVDIAVKRRVAETRDSKAKLEEHLAQVLAEVKDMEVNIDKLESAIAEKEQPLKVAETRLKVRGARPNVEQCRDPAQF----RLVEEVGGIQA-----------SVEALSQRLAASRDSLKGLLRRQLDLEEEIQIKANTLYIDEVQCGGLRGSIQIHSF 402          
BLAST of SMED30027478 vs. Ensembl Cavefish
Match: tekt3 (tektin 3 [Source:NCBI gene;Acc:103037718])

HSP 1 Score: 254.603 bits (649), Expect = 3.364e-79
Identity = 150/403 (37.22%), Postives = 237/403 (58.81%), Query Frame = -3
Query:   66 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESI 1274
            RY   +W  SN   YR +E  R +AERL+ +  RLI + D+ T  +    +K + +R++DI +W++EL  ++ ++  E   L E  +R+ +A      PL  S++C+ +RE R++IDLVHD V+K+LIKEVEV    +E + R++++A+ QL  NR A++ L+RD++DK+TAQ ID+   +++  S  I Y      +DP    P+ W  FT++NIL    +   S  +R  I+ +L  TA++   Q  + + +F  RI+E+ ++K  L+ HLA+ L+EI   E  I+ L+ AI  K  PLK+AQTRL+ R  RPNVE CRDNP +      + +V  I           E  I  L+ RLA+A +  + L + ++ LE +I VK NSLF+D+ +CM +R S 
Sbjct:   92 RYMPDDWFKSNYNNYRESESSRNSAERLRRDTLRLIQDRDQITRWTQESTSKNLGERLNDITFWRSELHHEIDNMVSEIAALTEVKRRLERALAETEGPLQTSQECLFHREKRMSIDLVHDGVEKDLIKEVEVIKSCQERMRRSLDKAIAQLASNRAAQHELERDLNDKVTAQRIDDRCHQMRNTSDGISYYRGIDRLDPSLSLPDSWSKFTDDNILLSQSERSTSHKLRDEIEVLLNTTANEMWNQFNSANVSFTNRISEMAEAKNSLQTHLAKTLQEIFQTEMLIETLKKAIRDKENPLKVAQTRLEERTRRPNVELCRDNPHH----RLVGEVREI-----------EDSIYKLRERLAEAENTLQNLVKTKVTLEHDISVKANSLFLDQEKCMSMRRSF 479          
BLAST of SMED30027478 vs. Ensembl Cavefish
Match: tekt4 (tektin 4 [Source:NCBI gene;Acc:103034475])

HSP 1 Score: 169.859 bits (429), Expect = 1.527e-47
Identity = 133/400 (33.25%), Postives = 204/400 (51.00%), Query Frame = -3
Query:   75 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIR 1274
            +Y+  EW  +N+   + A  + Q A+R+      L  E    T  + +     +  R+ D+   + EL +    +  E   L +H +R+ KA      PL IS   +  RE R   DLV D V++EL+KEVE+   V+ LL +T++QAV+Q+RLNR+A+  L+ D  DK  A  +D+        S D  +  ++A +  +      W  FT  N+   +RQ   S  +R + D VLQ+TA+D + Q   VD +F +R  E+  +K +LE  L RVL EI + E  I  L+ A+  K  PL++AQ+RL  R  RPN+E CRD PQ S   S + Q+                 +  LQ++L++A      L+  +L LE+ I  K NSLFID  +C+  R
Sbjct:   37 KYSPREWQENNSATLQRALTDHQHAQRVLRASRALSAEAAAGTQDAQSRGTLLLGARLQDVHSRRTELQRGAERLQAETELLQDHKRRLEKALLTTDLPLTISTDNLTCRENRFGPDLVQDPVEEELLKEVELIRNVQALLKKTLQQAVDQIRLNREAKQTLELDWSDKHEAYILDDQCGRYGNRSTDTQHHPSSARLHDQVCDERAWLKFTQVNLEEAERQYRCSVSLRELCDRVLQETAEDLRAQCAVVDQSFNQRCLELSQAKNQLELQLQRVLDEIGSQERNISTLQQAVYDKDTPLRVAQSRLHYRAQRPNMELCRDQPQISLE-SEVEQISG--------------SVGVLQQQLSEARQSLAQLEESRLMLEKNISCKTNSLFIDRDKCLTHR 421          
BLAST of SMED30027478 vs. Ensembl Cavefish
Match: tekt2 (tektin 2 [Source:NCBI gene;Acc:103026688])

HSP 1 Score: 165.622 bits (418), Expect = 4.126e-46
Identity = 115/398 (28.89%), Postives = 201/398 (50.50%), Query Frame = -3
Query:   96 VKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDE 1289
            +KP +R   P+W  SN      AE  R  +  ++ E   L +ET  +T     D +++++ RI D+  WK  L+  + DV  E   L    +   +A  A + PL ++ +C   REGR   +LV D V+ EL KEV+V +G + +L + I+Q+ EQL L ++A ++L  D+  K  A  +D     L   S +I        + P + TP+ W+ F++ N++    ++  S  +R  I     Q  ++ + Q+IA + A R+R + +  +  +++  L     EI+ +E  I+ LE  ++AK+ PLKL  TRL+ R  RP V+ CRD  Q+     A  Q+D+               I  L+++LA A    ++L+  +  + E++  KQ +L +++
Sbjct:    6 MKPGLRCRVPDWENSNKLISDTAEHRRHVSHEIRQEGRALRNETANKTSWDEYDSSRRLNDRISDVTRWKKSLEACVQDVNAEIDALTLAKEETERALAATVLPLEVTAECQTLREGRRGSELVSDPVEAELKKEVDVIDGAQRVLQQLIDQSFEQLCLLQEAHHQLTLDLQHKTEALDVDMSCLSLTVTSPEISLKPNPIRVPPGSTTPQQWEQFSHHNVIRAKEEMQTSLQLRENISITRAQVQNELEAQRIATEFALRKRTHHMEQALQEVQWQLKTTQDEITEMERDIRGLERDMQAKMAPLKLVHTRLENRTKRPGVDLCRDEVQFGLVEEA-KQLDST--------------IQTLKQKLAQALDSLQSLQHHEAQMLEDLSRKQEALALEQ 388          
BLAST of SMED30027478 vs. Ensembl Cavefish
Match: ENSAMXT00000039358.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000527.1:2505:3258:-1 gene:ENSAMXG00000034916.1 transcript:ENSAMXT00000039358.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 47.7506 bits (112), Expect = 7.266e-7
Identity = 21/63 (33.33%), Postives = 36/63 (57.14%), Query Frame = -3
Query: 1113 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKI 1301
            M  +++P  ++   EW ++N   +  AE ER  +ERL  EC RL++E++K   +   D N K+
Sbjct:    1 MPRVMEPTHKFLPSEWKHANQVHFGIAEAERARSERLTAECQRLVEESEKSVRRMQQDANMKL 63          
BLAST of SMED30027478 vs. Ensembl Sea Lamprey
Match: TEKT2 (tektin 2 [Source:HGNC Symbol;Acc:HGNC:11725])

HSP 1 Score: 87.4261 bits (215), Expect = 4.511e-20
Identity = 61/204 (29.90%), Postives = 104/204 (50.98%), Query Frame = -3
Query:   54 TPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKIEA 665
            +P  W  F+  N+     ++  SS +R  I   + QT ++ + QK + D AFR+R +E+   K +LE       +EIS +E  I +LE  + AK+ PLK+A TRL+ R  R  VE CRD PQY  + + + Q++                IA L+++L+D+ +    L+     +E ++  K  +L + E  C+ +R  + + A
Sbjct:    3 SPRTWDEFSRRNVERAMAEMRASSALREAITATIAQTTNELEAQKNSTDFAFRKRSHELDKVKAELEWQKKNNKQEISVLEGDIAHLEADVRAKMLPLKVAHTRLETRTYRSGVELCRDEPQYGIT-TEVHQIE--------------ATIATLKKKLSDSYNALTGLRCSLERVERDLASKALALGL-ERRCVDVRRKLTVSA 190          
BLAST of SMED30027478 vs. Ensembl Sea Lamprey
Match: TEKT2 (tektin 2 [Source:HGNC Symbol;Acc:HGNC:11725])

HSP 1 Score: 82.4185 bits (202), Expect = 1.658e-18
Identity = 59/196 (30.10%), Postives = 95/196 (48.47%), Query Frame = -3
Query:  702 VKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGY 1289
            VKP  R+T  EW  S       A+  R  +  ++ E   L D+++ +T    +D N ++++R+  ++ W   L   L D   E   L +      +A +A   PL ++ +C+  R+ R  +DLVHD V+ EL +EV+     KE L R+I  A  Q+   ++A  +L+ D+ DK  A  ID   R L   S  I  
Sbjct:    6 VKPGRRFTVGEWHDSGERVSSGAQSRRDVSRSVRQEARHLRDQSNNQTKWDQHDSNTRLEERMSSVQRWVERLQHTLDDTDAEINALAKTKDDAERALQAKAVPLQVATECLFLRDARRGVDLVHDPVEDELHREVKAIERAKEALTRSINDAFLQVCQLQEARQQLQGDVRDKEEALEIDSTCRALNNASATISL 201          
BLAST of SMED30027478 vs. Ensembl Nematostella
Match: EDO39292 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SAK1])

HSP 1 Score: 331.643 bits (849), Expect = 1.130e-110
Identity = 190/412 (46.12%), Postives = 265/412 (64.32%), Query Frame = -3
Query:   66 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESI 1301
            MA LV+PP +Y   EW  SN+ +Y  AE ER  AERL++E +RL  E + RTV++  DVNKK+DQRI DI +WK+ELD+  ++   E   +     R+ +A      PL I++QC+ NRE R+ IDLVHD+V+ +L+KEVEV  GV+ LL RTIEQA EQ+RL R A Y L++D  DK  A  ID     LK +S  + Y   A +I+  +VTP++W+ F+N+N+L  +R+  +S+ +RSIIDG+L QT +D   Q  AV+ AF +RI E   +K KLE+HL +V +EI+ +E+ I  L+ AI  KI P+K+AQTR++ R  RPN+E CRD  QY      I +V  I+            +I  LQ +L +A S  KAL RRQL LEE+I+VK+NSLFID  +CM +R+ I
Sbjct:    1 MARLVQPPPKYVPEEWHASNHLQYFTAEKERAAAERLRDESERLRVEIEVRTVETQEDVNKKLDQRITDINFWKSELDRNQSETEEEINMMLNFKARLERALADTELPLMIAQQCLMNREKRIKIDLVHDNVEMQLLKEVEVIEGVQALLKRTIEQATEQIRLLRSARYYLEKDRKDKFHANKIDNTCAALKNDSPGLVYARDAVKIEKNSVTPDEWEDFSNKNMLKAEREKNSSTSLRSIIDGLLVQTLNDLMNQCRAVNLAFEKRIEETLKAKTKLEDHLMKVKEEIAAMEENIATLQRAIALKIAPMKVAQTRVENRTFRPNIELCRDRVQY----RLIDEVVEIST-----------NIERLQEKLYEAESSLKALIRRQLSLEEDIEVKENSLFIDRDQCMELRKQI 397          
BLAST of SMED30027478 vs. Ensembl Nematostella
Match: EDO40681 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S6M2])

HSP 1 Score: 270.396 bits (690), Expect = 5.307e-87
Identity = 162/388 (41.75%), Postives = 236/388 (60.82%), Query Frame = -3
Query:   63 NAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIK 1226
            + E ER TAERL++EC RL  ET   T ++  DVNKK+D R+ DI YWK EL+K+ ++   E   L   I R+ KA  A  +PL+IS+QC+  RE RV IDLVHD+V+  L KEVE+   V+ LL +T++QA EQL L R A+ +L++D+ DK  A GID     L  +SKDI +   A  I   + TP++W+++++ENI   +R+   S  +RS I+G+L QT  D +     V+  F +RI +   +K  LE+ L+RV+ E++++++ I+ L  AI  K  PLK+A TRL  R  RPNVE CRD  QY      + +V  I              I  L+ RLA+A +  +AL R + +LE++I+VK NSLFID  +CM +R+ +K
Sbjct:    6 SGEKERATAERLRDECSRLRTETSLLTHRTQMDVNKKLDHRLHDINYWKAELEKQHSETVAEIKALQAFIGRLEKALAATEKPLNISQQCLGYRERRVKIDLVHDNVETHLSKEVEMIENVQALLKKTMDQAHEQLWLLRSAKTQLEKDLGDKYGALGIDGKCSTLNNHSKDIHFAPHAVTIQQHSFTPDEWEAYSSENIAKAERERKASVALRSEINGILMQTYVDLRNIYETVNNEFHKRIEDTVGAKKSLEKELSRVMDELASMDNNIRTLTQAIADKEAPLKVAHTRLDKRSIRPNVELCRDPVQY----RLVGEVGEINT-----------SIDRLRMRLAEAQASMQALLRNKENLEDDIEVKTNSLFIDRDQCMVVRQQVK 378          
BLAST of SMED30027478 vs. Ensembl Nematostella
Match: EDO37357 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SG13])

HSP 1 Score: 231.491 bits (589), Expect = 1.181e-71
Identity = 151/412 (36.65%), Postives = 227/412 (55.10%), Query Frame = -3
Query:   63 LVKP--PMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIK 1292
            L  P  P R+   +W  SN     NAE +R+ + R++ E   L +ETD  T  + +D N K+++RIDDI  WK  L++ LA+   E   L    +R  +A EA   PL I+ +C+  RE R +IDLV D+V+ +L KEVEV  G K LL + ++++ EQL L ++A ++L  D+ DK T   ID     +   S +IGY          +VTPE W SF+N N L  + ++  S  +R  I   LQQTA+D + Q+ A + +FR+RI+E   +K +LE      +KEI+ +ED IK L+ AI+AK  P+ +AQTRL+ R  RPNVE CRD PQY   C  + ++                 +  L  +L +A +  +AL+     + E++  K NSL +D   CM +RE ++
Sbjct:    3 LTNPNIPPRFAVSDWHTSNQIIRTNAERQREASHRVRQESRFLRNETDNHTRWTQHDSNTKLEKRIDDINDWKRSLERCLAETDNEIALLTREKERTERALEAKKVPLDITLECLMLRENRQSIDLVRDEVEAQLHKEVEVIEGTKALLQQKVDESFEQLCLLQEARHQLHLDITDKFTTLQIDGDCYAMNNMSTNIGYQVNPTRTVKGSVTPETWDSFSNYNKLRAEAEMKASKHLREAIFATLQQTANDLEAQRKASEYSFRKRIHETDQAKSELEWQQKNTMKEIATLEDDIKGLKEAIDAKNAPMMVAQTRLENRTYRPNVELCRDQPQYQ-LCHEVAEI--------------AGSMRALTEKLREAQNSLEALRSNLSRINEDLAFKNNSLALDN-RCMSVREKMR 398          
BLAST of SMED30027478 vs. Ensembl Medaka
Match: tekt3 (tektin 3 [Source:NCBI gene;Acc:101160513])

HSP 1 Score: 248.44 bits (633), Expect = 6.931e-77
Identity = 144/403 (35.73%), Postives = 237/403 (58.81%), Query Frame = -3
Query:   66 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESI 1274
            RYT  +W  SN + Y  +E  R++AERL+ +  RLI + D+ T ++  + +K I +R++DI++WK+EL  ++ ++  E   L E  +R+ +A      PL ++++C+ +RE R++IDLVHD V+K+LIK +EV    +E +   +++A  QL  NR A++ L++D+ DKLTAQ ID+    L+  S  IGY       D     P+ W  FT++NIL    +   S  +R  I+ +L  T+++   Q   V+ AF  RI+E  D+K  L+ HLA+ ++EI   E  I++L+ AI  K  PLK+AQTRL+ R  RPN+E CRDNP Y    + + ++++               I  L  RL +A +  +AL + ++ LE ++ +K NSLF+D+ +CM +R++ 
Sbjct:   90 RYTPDDWYKSNQSHYWESESSRKSAERLRKDTMRLIQDKDQLTRRTQENTSKNISERLNDIRFWKSELSHEIDNMVTEIAALTEVKRRLERALAETQGPLQVAQECLYHREKRMSIDLVHDGVEKDLIK-IEVIKSCQERMRLHLDRATSQLASNRAAQHELEKDLSDKLTAQRIDDRCHHLRNTSDGIGYYRGIDRQDHAVSLPDSWSRFTDDNILRSQSERATSHKLRDEIEILLNTTSNEMWNQFNNVNVAFTNRISETTDAKSSLQAHLAKTMQEIFQTEMLIESLKKAIRDKENPLKVAQTRLEERTRRPNIELCRDNP-YHRLVTEVREIEDT--------------IQKLHERLMEAENTLQALVKTKVKLENDLSIKANSLFLDQEKCMSMRKTF 476          
BLAST of SMED30027478 vs. Ensembl Medaka
Match: tekt1 (tektin-1 [Source:NCBI gene;Acc:101161072])

HSP 1 Score: 172.94 bits (437), Expect = 4.274e-49
Identity = 128/388 (32.99%), Postives = 207/388 (53.35%), Query Frame = -3
Query:   51 AERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEF---VRELKPNSKDIGYTNAAAEIDPKAV-TPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKIEAY 1202
            +ER + EC  LI ET +   +   D N++ DQ++  I++ K EL+ KL +V +E   L     RV KA +A  EPL ++  CM  R  R   +  HD+V  EL+KE E    V+ LL R  EQ  EQLRLNR A+Y +++D+ +K  AQ +D F   +  L  + +    T++  E  P +V T + W++ +  N+   ++Q  NS  ++ ++  VL QTA D ++Q  A  TA +  + +++++K ++E+ L ++  EIS+   T ++L +A+    + L L Q RL +R  RP  E  RD  Q       +  V  IT           + I  LQ+ +  +  E++ L   +L L+E I  K N L+IDEV CM  RE++ I+ +
Sbjct:   23 SERFRAECKELIKETKQICKRMQEDENQQFDQKLRRIQFLKKELELKLDEVNLEIDDLVVLQSRVTKALDACKEPLEVTDLCMKERLKRPNSERFHDEVDGELLKESEGLKRVESLLQRVTEQISEQLRLNRSAKYGIEKDLKEKSEAQNVDNFCTLMATLSMSHRHRPKTHSTPE--PSSVLTLDQWENISYANMAKAEQQKTNSRSLQVLVQSVLDQTAADMQKQVQATTTAIQLNVQKMKNAKNQMEDELVKIQSEISSQRKTREDLRVAVAENEEFLSLTQARLALRHERPGKEQWRDPAQR----KLLADVQKIT-----------EHIKQLQQEVDRSEDEERRLLNWKLRLQESIDSKANCLYIDEVVCMQRREAVIIQNF 393          
BLAST of SMED30027478 vs. Ensembl Medaka
Match: tekt4 (tektin 4 [Source:NCBI gene;Acc:101173527])

HSP 1 Score: 158.688 bits (400), Expect = 2.456e-43
Identity = 118/410 (28.78%), Postives = 204/410 (49.76%), Query Frame = -3
Query:   75 DMAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIR 1304
            D A  V    +Y+  EW  +  T    A  +   AE +Q     L  +++  T+++  +  + + +R+ +I +W++EL + +  +  +   L     R+ +A EA   P  I+   +  R  R   DLV D V++EL+KEV +   ++ LL RTI Q   Q+R N  A+  L+ D  DK  A  +DE        S D     ++A    +   P  W  FT +N+L   ++   S  +R++++ +L++T +D   Q+  VD AF +R  E+ ++K +L+  L +VL++I   E  I  L+ AI  K  PL++ Q+RL +R  RPN+E CRD  Q S    A  Q++                +  L+++L +A      L+  +++L+++I  K  SLFI+  +CM  R
Sbjct:   38 DSAAGVGRSTKYSPAEWFSNYQTILVRAGADNLEAESIQQLSRTLNRDSEGVTLQTQAEGTRLLGERLQEIHHWRSELLRHIEQLQADMEALQAARTRLERALEATDTPYAIATDNLNCRAKRPGPDLVQDSVEEELLKEVGLIRNIQALLKRTIAQVASQIRRNMDAKQVLEMDWSDKQEAYNLDERGGGHSNRSPDTQLHPSSAANHEQVCNPTTWTKFTQDNLLQAAQEEEASRRLRALVEEMLRETTEDLINQRSTVDGAFSQRCQELLEAKTELQIKLTQVLQQIGEQEKNIVGLQKAIHLKEAPLRVVQSRLHLRSLRPNMELCRDPAQLSLEAEAA-QIETT--------------LNSLKQKLNEARISLSNLEESRMELQKDISCKSQSLFIEGEKCMTQR 432          
BLAST of SMED30027478 vs. Ensembl Medaka
Match: tekt2 (tektin 2 [Source:NCBI gene;Acc:101164907])

HSP 1 Score: 157.918 bits (398), Expect = 3.726e-43
Identity = 101/332 (30.42%), Postives = 176/332 (53.01%), Query Frame = -3
Query:  294 VKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYS 1289
            VKP +R    EW  +N   +  A+ +R  +  ++ EC  L +ET  +TV    + ++++ +R+ D+  W++ LD     V  E   L    +   +A  A   PL +S +CM  REGR   +LV D VQ++L KEVE+   V++ L + I++A +QL + ++A ++L  D+ +K+ A  ID+    L   S +I        I   A TP++W   +N N++  +  +  S  MR  +     Q  ++ + ++ A + A R+R +    ++ +L+  +  +  EI+ +E  I+ L+  ++AK  PLKLA TRL+ R  RP ++ CRD  QYS
Sbjct:    4 VKPSLRCGVSEWNNNNLQLWAEAQQQRLVSSEIRQECKSLRNETHCKTVWDEKETSRRLTERVWDVSRWRDLLDSCAQKVDEEMDALTLSKELTEQALAATATPLEVSTECMMLREGRRGYELVTDPVQEQLQKEVELIERVQQTLQQHIDEAFQQLCVLQEARHQLTGDLQNKMEALDIDKSCLTLTITSPNISLKTNPTRIPSGASTPQEWVQLSNHNVVQAEEAMRVSQQMREDMSLSRAQLQNELEAERRATEFALRKRSHHEEQARDELQWQIRNMEAEIAEMESDIRELDADLQAKTAPLKLAHTRLENRTHRPGMDLCRDEVQYS 335          
BLAST of SMED30027478 vs. Planmine SMEST
Match: SMESG000081245.1 (SMESG000081245.1)

HSP 1 Score: 784.637 bits (2025), Expect = 0.000e+0
Identity = 394/417 (94.48%), Postives = 398/417 (95.44%), Query Frame = -3
Query:   51 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKIEAY 1301
            MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQY      + +V+ +           EKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKIEAY
Sbjct:    1 MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQY----RLLEEVEEL-----------EKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKIEAY 402          
BLAST of SMED30027478 vs. Planmine SMEST
Match: SMESG000065257.1 (SMESG000065257.1)

HSP 1 Score: 259.61 bits (662), Expect = 4.454e-83
Identity = 149/361 (41.27%), Postives = 221/361 (61.22%), Query Frame = -3
Query:   51 NKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQ---YSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKIEAY 1124
            NKK+ QRIDD+KYW +E +KK  ++  E   L     R+ KAR A+ + LH +K C  NRE R  IDL  DDV   L KE+E +  V +LLD++ E+A EQLRLNRKA +RL +D  +K  +  ID +  + KP+  DI  T   ++ +   +  +DW   TN+NI N +++ +NS +MRSIIDG+LQQ A+D +R     + +FR+RI+E++D+KGK EEH+ RVL+EI N E ++++L+  +++K+  L LA+ RL  R  R   ENC D P    +   C                    + DI  L++R+ D   + K L+R+QL LEE+IQ K NS+FIDEV C GIRE++ ++++
Sbjct:    4 NKKLAQRIDDVKYWNDENEKKSDELFKETDMLGTSAARIDKARAAVKDYLHYTKACKVNREDRYGIDLCADDVHFNLNKEIETYENVLKLLDKSEEEAREQLRLNRKANHRLYQDSLNKKASLAIDNYCLDSKPHLSDINQTPPNSDPNKPLIQLDDWMHLTNDNIHNGEKERMNSENMRSIIDGILQQVANDMERVVEETNVSFRKRISELKDTKGKDEEHMNRVLQEIKNTETSMEDLKKCLDSKMAYLTLAEHRLGQRNLRIANENCLDEPHIRLHEEICEI------------------KLDIIQLKKRIVDHEFQLKGLRRQQLGLEEDIQCKSNSIFIDEVICGGIRETLVVKSF 346          
BLAST of SMED30027478 vs. Planmine SMEST
Match: SMESG000020792.1 (SMESG000020792.1)

HSP 1 Score: 219.55 bits (558), Expect = 7.147e-66
Identity = 135/405 (33.33%), Postives = 221/405 (54.57%), Query Frame = -3
Query:   60 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKI 1274
            RYT  +W  S    Y  ++  R  AE+++ +  R+  E D RT ++  D  KK+  R+ DI + +NE++ ++  +  E+  L    +   K+      PLH++++C+ NRE R  IDLVHD V+K L+ EV++    ++ +   + +A  QL LNR +++ L++D  DK  A+ ID + + LK +S  I Y N    +D    TPE W  FTN N+     +   S  +R  I+ VL +T  D  ++   V+ A  +R  E  D++ K++ HL +VL+EI ++E  I+ ++ +I+ K  PLK+AQTRL VR  RPN+E CRD P+       I +V  I            + I +L R+L  A +  + L   + +LE ++ VK NSLFID  +C+G R++  +
Sbjct:   91 RYTPNDWIKSAEINYNVSDNVRNNAEKIRLDTSRMAREFDDRTKRAQEDSGKKLGDRLADINFMRNEVNDEIDKMMAESEALSNAKRAAEKSLSETENPLHVTQECLYNREKRSGIDLVHDMVEKNLLGEVDLIKKCQDQIAELVNKANVQLNLNRASQHELEKDSHDKFQAEQIDSYAQSLKNSSPGIAYYNGVENVDNCISTPETWLKFTNNNLQRSAAEREASRRLREEIEQVLARTQRDMAKKWAEVNAALSQRAQETADARNKIQNHLNKVLQEIFDMEKNIEYIKKSIQDKEAPLKVAQTRLHVRLDRPNMEACRDQPR----SRLIEEVHEI-----------RESINNLNRQLQQAEATMQHLLSSKSNLEHDLNVKTNSLFIDREKCLGTRKTFPM 480          
BLAST of SMED30027478 vs. Planmine SMEST
Match: SMESG000021346.1 (SMESG000021346.1)

HSP 1 Score: 219.55 bits (558), Expect = 7.745e-66
Identity = 138/407 (33.91%), Postives = 227/407 (55.77%), Query Frame = -3
Query:   57 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLE-PLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKIE 1274
            RYT  +W  +    +++A+  R   ER++ + +RL  ETD +T ++  DV KKI  R+ D  +WK ELD ++  +  EN  L+E +KRV +      E  LHI ++C+  RE R  IDLVHD+V+K LI E++     ++ +   I +A  Q+ LNR A++  + D  DK  AQ IDE   ++K +S  I + N    ID    TPE W  ++++N+     +  NS+ +R  I+  L + A+D  ++   V+TA   RI E  D++ K++ HLA++L+EI ++E  I+ L+  I+ K   +K+AQTRL  R  RP +E CRD P +      + +VD I              +A L R L +A +  + L   +  LE+++ +K N+LF+D  +C+G+R++  I+
Sbjct:  101 RYTPKDWMTAAMKTFQSADRVRDYGERIRFDTNRLGRETDDQTKRAQEDVGKKIGDRLSDTNFWKTELDDEIDKMITENNNLNE-MKRVAEKMLGETENQLHIGEECLYTREKRQGIDLVHDNVEKSLIGEIDTVKQAQDDIRNIISKANAQMSLNRAAQHENELDSRDKFIAQQIDENAHQIKNSSAGINFHNGVETIDNCVSTPETWAKYSDDNVKRSQAERENSTKVREEIEATLDKAANDMWKRWNNVNTALTERIQETIDARNKIQAHLAKILQEIFDMEKNIEYLKKCIKDKEDYMKVAQTRLHFRLRRPGMELCRDQPNH----RLVEEVDEIREV-----------VAMLMRELREAETALQHLLDNKAVLEQDLNIKNNTLFLDREKCLGLRKTFPIQ 491          
BLAST of SMED30027478 vs. Planmine SMEST
Match: SMESG000021346.1 (SMESG000021346.1)

HSP 1 Score: 219.935 bits (559), Expect = 8.804e-66
Identity = 138/407 (33.91%), Postives = 227/407 (55.77%), Query Frame = -3
Query:   57 RYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDKRTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGKAREALLE-PLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELLDRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGYTNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTADDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLEMAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITXXXXXXXXXXEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFIDEVECMGIRESIKIE 1274
            RYT  +W  +    +++A+  R   ER++ + +RL  ETD +T ++  DV KKI  R+ D  +WK ELD ++  +  EN  L+E +KRV +      E  LHI ++C+  RE R  IDLVHD+V+K LI E++     ++ +   I +A  Q+ LNR A++  + D  DK  AQ IDE   ++K +S  I + N    ID    TPE W  ++++N+     +  NS+ +R  I+  L + A+D  ++   V+TA   RI E  D++ K++ HLA++L+EI ++E  I+ L+  I+ K   +K+AQTRL  R  RP +E CRD P +      + +VD I              +A L R L +A +  + L   +  LE+++ +K N+LF+D  +C+G+R++  I+
Sbjct:  113 RYTPKDWMTAAMKTFQSADRVRDYGERIRFDTNRLGRETDDQTKRAQEDVGKKIGDRLSDTNFWKTELDDEIDKMITENNNLNE-MKRVAEKMLGETENQLHIGEECLYTREKRQGIDLVHDNVEKSLIGEIDTVKQAQDDIRNIISKANAQMSLNRAAQHENELDSRDKFIAQQIDENAHQIKNSSAGINFHNGVETIDNCVSTPETWAKYSDDNVKRSQAERENSTKVREEIEATLDKAANDMWKRWNNVNTALTERIQETIDARNKIQAHLAKILQEIFDMEKNIEYLKKCIKDKEDYMKVAQTRLHFRLRRPGMELCRDQPNH----RLVEEVDEIREV-----------VAMLMRELREAETALQHLLDNKAVLEQDLNIKNNTLFLDREKCLGLRKTFPIQ 503          
The following BLAST results are available for this feature:
BLAST of SMED30027478 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TEKT12.439e-11043.72tektin 1 [Source:HGNC Symbol;Acc:HGNC:15534][more]
TEKT33.665e-6934.33tektin 3 [Source:HGNC Symbol;Acc:HGNC:14293][more]
TEKT33.665e-6934.33tektin 3 [Source:HGNC Symbol;Acc:HGNC:14293][more]
TEKT52.140e-6734.99tektin 5 [Source:HGNC Symbol;Acc:HGNC:26554][more]
TEKT45.995e-6032.50tektin 4 [Source:HGNC Symbol;Acc:HGNC:31012][more]
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BLAST of SMED30027478 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
R02E12.43.139e-1523.92pep chromosome:WBcel235:X:4005314:4007634:1 gene:W... [more]
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BLAST of SMED30027478 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG328194.143e-5430.12gene:FBgn0052819 transcript:FBtr0070051[more]
CG328204.143e-5430.12gene:FBgn0052820 transcript:FBtr0070054[more]
CG174504.143e-5430.12gene:FBgn0040028 transcript:FBtr0345920[more]
CG174504.143e-5430.12gene:FBgn0040028 transcript:FBtr0070056[more]
CG328192.661e-5330.12gene:FBgn0052819 transcript:FBtr0070050[more]
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BLAST of SMED30027478 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tekt11.104e-9341.49tektin 1 [Source:ZFIN;Acc:ZDB-GENE-041114-99][more]
tekt33.729e-8036.63tektin 3 [Source:ZFIN;Acc:ZDB-GENE-080130-2][more]
tekt33.821e-8036.63tektin 3 [Source:ZFIN;Acc:ZDB-GENE-080130-2][more]
tekt33.821e-8036.63tektin 3 [Source:ZFIN;Acc:ZDB-GENE-080130-2][more]
tekt41.389e-5032.25tektin 4 [Source:ZFIN;Acc:ZDB-GENE-070410-98][more]
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BLAST of SMED30027478 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
gdf104.854e-11747.00growth differentiation factor 10 [Source:Xenbase;A... [more]
tekt31.547e-7937.72tektin 3 [Source:NCBI gene;Acc:549229][more]
plcd12.162e-6735.00phospholipase C delta 1 [Source:Xenbase;Acc:XB-GEN... [more]
fam168a8.942e-5841.44family with sequence similarity 168 member A [Sour... [more]
fam168a1.785e-5340.22family with sequence similarity 168 member A [Sour... [more]
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BLAST of SMED30027478 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tekt17.821e-10942.96tektin 1 [Source:MGI Symbol;Acc:MGI:1333819][more]
Tekt17.821e-10942.96tektin 1 [Source:MGI Symbol;Acc:MGI:1333819][more]
Tekt17.821e-10942.96tektin 1 [Source:MGI Symbol;Acc:MGI:1333819][more]
Tekt58.687e-6634.49tektin 5 [Source:MGI Symbol;Acc:MGI:1917676][more]
Tekt47.084e-6534.00tektin 4 [Source:MGI Symbol;Acc:MGI:1919090][more]
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BLAST of SMED30027478 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q99JD2|TEKT1_RAT9.849e-11044.66Tektin-1 OS=Rattus norvegicus OX=10116 GN=Tekt1 PE... [more]
sp|Q969V4|TEKT1_HUMAN1.171e-10943.72Tektin-1 OS=Homo sapiens OX=9606 GN=TEKT1 PE=1 SV=... [more]
sp|Q32KZ9|TEKT1_BOVIN5.451e-10944.17Tektin-1 OS=Bos taurus OX=9913 GN=TEKT1 PE=2 SV=1[more]
sp|Q9DAJ2|TEKT1_MOUSE5.470e-10842.96Tektin-1 OS=Mus musculus OX=10090 GN=Tekt1 PE=1 SV... [more]
sp|Q9TTW3|TEKT1_CANLF2.478e-10341.61Tektin-1 OS=Canis lupus familiaris OX=9615 GN=TEKT... [more]
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BLAST of SMED30027478 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4S2M0K22.906e-14955.40Uncharacterized protein OS=Opisthorchis felineus O... [more]
G4V8P12.301e-14753.72Putative tektin OS=Schistosoma mansoni OX=6183 GN=... [more]
A0A4Z2DU155.548e-14654.68Tektin-1 isoform 2 OS=Schistosoma japonicum OX=618... [more]
A0A183K5D91.010e-14553.59Uncharacterized protein OS=Schistosoma curassoni O... [more]
A0A1I8GZC71.860e-14553.48Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of SMED30027478 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
tekt33.364e-7937.22tektin 3 [Source:NCBI gene;Acc:103037718][more]
tekt41.527e-4733.25tektin 4 [Source:NCBI gene;Acc:103034475][more]
tekt24.126e-4628.89tektin 2 [Source:NCBI gene;Acc:103026688][more]
ENSAMXT00000039358.17.266e-733.33pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
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BLAST of SMED30027478 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
TEKT24.511e-2029.90tektin 2 [Source:HGNC Symbol;Acc:HGNC:11725][more]
TEKT21.658e-1830.10tektin 2 [Source:HGNC Symbol;Acc:HGNC:11725][more]
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BLAST of SMED30027478 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027478 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO392921.130e-11046.12Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO406815.307e-8741.75Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO373571.181e-7136.65Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30027478 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 4
Match NameE-valueIdentityDescription
tekt36.931e-7735.73tektin 3 [Source:NCBI gene;Acc:101160513][more]
tekt14.274e-4932.99tektin-1 [Source:NCBI gene;Acc:101161072][more]
tekt42.456e-4328.78tektin 4 [Source:NCBI gene;Acc:101173527][more]
tekt23.726e-4330.42tektin 2 [Source:NCBI gene;Acc:101164907][more]
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BLAST of SMED30027478 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000081245.10.000e+094.48SMESG000081245.1[more]
SMESG000065257.14.454e-8341.27SMESG000065257.1[more]
SMESG000020792.17.147e-6633.33SMESG000020792.1[more]
SMESG000021346.17.745e-6633.91SMESG000021346.1[more]
SMESG000021346.18.804e-6633.91SMESG000021346.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027478 ID=SMED30027478|Name=SMED30027478|organism=Schmidtea mediterranea sexual|type=transcript|length=1354bp
ATTTTATTTAGAAACACATTCAATATTTTTGTTTTTAATCTTAGCGATTA
ATATGCTTCAATTTTTATACTTTCTCTTATTCCCATACATTCAACTTCGT
CAATAAATAAAGAATTTTGCTTCACTTGGATTTCCTCTTCCAAGTCCAAT
TGTCGTCGTTTTAAAGCTTTCTTTTCACTATTAGCATCAGCTAGACGTCT
TTGAAGATGAGCAATATCCTTTTCCAATTCTTCTACTTCTTCCAACAGTC
GTAGAGTAATATTATCAACTTGAGGAATTGCACTACAAGAATTGCTGTAT
TGAGGATTATCACGACAATTTTCAACATTCGGTCGTCCTTTACGTACTTG
CAATCTCGTTTGTGCCAATTTCAATGGCTGAATTTTAGCTTCAATAGCCA
TTTCAAGATTTTTGATGGTATCTTCAACGTTAGAAATTTCTTTAAGTACT
CTGGCAAGATGTTCTTCCAACTTTCCTTTAGAATCTCTGACTTCGTTAAT
TCTTCGTCGAAATGCCGTATCAACGGCAATTTTCTGTCTCTTTTGATCAT
CCGCTGTTTGTTGAAGAACTCCATCAATTATAGACCTCATGTCACTGGAA
TTGAGTAACTGTCGATCCATGTTGAGGATATTTTCATTTGTAAACGACTG
CCAGTCCTCGGGAGTTACCGCCTTTGGATCGATCTCTGCAGCAGCATTTG
TATAACCAATGTCTTTGGAATTCGGCTTCAATTCTCTGACAAATTCATCA
ATTCCTTGGGCAGTTAATTTGTCATCCATATCTCTTTTAAGTCGATATTC
AGCTTTACGGTTTAACCTTAATTGTTCAACTGCTTGTTCGATAGTTCTAT
CAAGCAATTCTTTTACGCCATTATGTACTTCAACTTCTTTAATCAGCTCT
TTCTGAACATCGTCGTGGACCAAATCTATTGCTACTCTTCCTTCACGATT
GGCCATACATTGTTTGGAAATATGTAAAGGTTCAAGTAAAGCTTCTCTGG
CCTTGCCAACTCGTTTAATGTGTTCGTCTAATTTATAATTTTCAACAGCG
ACATCGGCCAATTTTTTATCTAATTCATTTTTCCAGTATTTAATATCGTC
GATTCTTTGATCTATTTTTTTATTGACATCATTCAAGGATTTTACAGTTC
TTTTATCTGTTTCATCGATCAGCCTATCGCATTCATTTTGTAATCTTTCA
GCAGTTTGTCTTTCTATTTCAGCATTTCTATATTTTGTATTGTTGCTATA
AGTCCATTCTGGATGAGTATATCTCATTGGAGGTTTCACTAAATGAGCCA
TATCGTTTACAAAGAAAACTACTTCAAAAAAATGTTACAACAGAATACTT
GAGA
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protein sequence of SMED30027478-orf-1

>SMED30027478-orf-1 ID=SMED30027478-orf-1|Name=SMED30027478-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=418bp
MAHLVKPPMRYTHPEWTYSNNTKYRNAEIERQTAERLQNECDRLIDETDK
RTVKSLNDVNKKIDQRIDDIKYWKNELDKKLADVAVENYKLDEHIKRVGK
AREALLEPLHISKQCMANREGRVAIDLVHDDVQKELIKEVEVHNGVKELL
DRTIEQAVEQLRLNRKAEYRLKRDMDDKLTAQGIDEFVRELKPNSKDIGY
TNAAAEIDPKAVTPEDWQSFTNENILNMDRQLLNSSDMRSIIDGVLQQTA
DDQKRQKIAVDTAFRRRINEVRDSKGKLEEHLARVLKEISNVEDTIKNLE
MAIEAKIQPLKLAQTRLQVRKGRPNVENCRDNPQYSNSCSAIPQVDNITL
RLLEEVEELEKDIAHLQRRLADANSEKKALKRRQLDLEEEIQVKQNSLFI
DEVECMGIRESIKIEAY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000106ciliated epithelial cell
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR000435Tektin