UPF0544 protein C5orf45 like protein

Overview
NameUPF0544 protein C5orf45 like protein
Smed IDSMED30027476
Length (bp)390
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of UPF0544 protein C5orf45 like protein (SMED30027476) t-SNE clustered cells

Violin plots show distribution of expression levels for UPF0544 protein C5orf45 like protein (SMED30027476) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of UPF0544 protein C5orf45 like protein (SMED30027476) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for UPF0544 protein C5orf45 like protein (SMED30027476) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30027476

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30027476SMESG000034778.1 SmedASXL_006061SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
epidermisSMED30027476SMESG000034778.1 dd_Smed_v4_7544_0_2dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30027476SMESG000034778.1 dd_Smed_v4_7544_0_2dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30027476SMESG000034778.1 dd_Smed_v4_7544_0_2dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027476SMESG000034778.1 Contig34038newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027476SMESG000034778.1 Contig34038uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Human
Match: MRNIP (MRN complex interacting protein [Source:HGNC Symbol;Acc:HGNC:30817])

HSP 1 Score: 62.003 bits (149), Expect = 2.968e-12
Identity = 38/112 (33.93%), Postives = 61/112 (54.46%), Query Frame = 2 
Query:   86 QIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGKI--------ENNFSHTEE---VPNESTSINQKIES----SKWNKYLEDENQ 376
            Q   V+ C  C++FQ+   +K  KW CK C  KQS ++ Y EG+G +CR+ VQ+ N   G++        E   S +EE      ++ ++ Q+ +S    S+W KYLE ++Q
Sbjct:    5 QRSRVLRCCSCRLFQAHQVKKSVKWTCKACGEKQSFLQAYGEGSGADCRRHVQKLNLLQGQVSELPLRSLEETVSASEEENVGHQQAGNVKQQEKSQPSESRWLKYLEKDSQ 116
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Human
Match: MRNIP (MRN complex interacting protein [Source:HGNC Symbol;Acc:HGNC:30817])

HSP 1 Score: 62.003 bits (149), Expect = 2.968e-12
Identity = 38/112 (33.93%), Postives = 61/112 (54.46%), Query Frame = 2 
Query:   86 QIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGKI--------ENNFSHTEE---VPNESTSINQKIES----SKWNKYLEDENQ 376
            Q   V+ C  C++FQ+   +K  KW CK C  KQS ++ Y EG+G +CR+ VQ+ N   G++        E   S +EE      ++ ++ Q+ +S    S+W KYLE ++Q
Sbjct:    5 QRSRVLRCCSCRLFQAHQVKKSVKWTCKACGEKQSFLQAYGEGSGADCRRHVQKLNLLQGQVSELPLRSLEETVSASEEENVGHQQAGNVKQQEKSQPSESRWLKYLEKDSQ 116
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Human
Match: MRNIP (MRN complex interacting protein [Source:HGNC Symbol;Acc:HGNC:30817])

HSP 1 Score: 62.003 bits (149), Expect = 5.112e-12
Identity = 38/112 (33.93%), Postives = 61/112 (54.46%), Query Frame = 2 
Query:   86 QIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGKI--------ENNFSHTEE---VPNESTSINQKIES----SKWNKYLEDENQ 376
            Q   V+ C  C++FQ+   +K  KW CK C  KQS ++ Y EG+G +CR+ VQ+ N   G++        E   S +EE      ++ ++ Q+ +S    S+W KYLE ++Q
Sbjct:    5 QRSRVLRCCSCRLFQAHQVKKSVKWTCKACGEKQSFLQAYGEGSGADCRRHVQKLNLLQGQVSELPLRSLEETVSASEEENVGHQQAGNVKQQEKSQPSESRWLKYLEKDSQ 116
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Human
Match: MRNIP (MRN complex interacting protein [Source:HGNC Symbol;Acc:HGNC:30817])

HSP 1 Score: 62.003 bits (149), Expect = 5.112e-12
Identity = 38/112 (33.93%), Postives = 61/112 (54.46%), Query Frame = 2 
Query:   86 QIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGKI--------ENNFSHTEE---VPNESTSINQKIES----SKWNKYLEDENQ 376
            Q   V+ C  C++FQ+   +K  KW CK C  KQS ++ Y EG+G +CR+ VQ+ N   G++        E   S +EE      ++ ++ Q+ +S    S+W KYLE ++Q
Sbjct:    5 QRSRVLRCCSCRLFQAHQVKKSVKWTCKACGEKQSFLQAYGEGSGADCRRHVQKLNLLQGQVSELPLRSLEETVSASEEENVGHQQAGNVKQQEKSQPSESRWLKYLEKDSQ 116
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Human
Match: MRNIP (MRN complex interacting protein [Source:HGNC Symbol;Acc:HGNC:30817])

HSP 1 Score: 62.3882 bits (150), Expect = 7.332e-12
Identity = 38/112 (33.93%), Postives = 61/112 (54.46%), Query Frame = 2 
Query:   86 QIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGKI--------ENNFSHTEE---VPNESTSINQKIES----SKWNKYLEDENQ 376
            Q   V+ C  C++FQ+   +K  KW CK C  KQS ++ Y EG+G +CR+ VQ+ N   G++        E   S +EE      ++ ++ Q+ +S    S+W KYLE ++Q
Sbjct:    5 QRSRVLRCCSCRLFQAHQVKKSVKWTCKACGEKQSFLQAYGEGSGADCRRHVQKLNLLQGQVSELPLRSLEETVSASEEENVGHQQAGNVKQQEKSQPSESRWLKYLEKDSQ 116
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Fly
Match: CG43295 (gene:FBgn0262987 transcript:FBtr0306825)

HSP 1 Score: 55.4546 bits (132), Expect = 3.261e-10
Identity = 28/62 (45.16%), Postives = 37/62 (59.68%), Query Frame = 2 
Query:   80 MSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALG 265
            MSQ I VV C +CK++Q    +K + W CK C  KQS++K +F G+  ECR  VQ  N   G
Sbjct:    1 MSQQIRVVQCIQCKMYQVDFVKKASTWQCKICRQKQSLLKEFFRGSAAECRVKVQHLNLERG 62
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Zebrafish
Match: mrnip (MRN complex interacting protein [Source:NCBI gene;Acc:641417])

HSP 1 Score: 51.2174 bits (121), Expect = 3.616e-8
Identity = 26/63 (41.27%), Postives = 36/63 (57.14%), Query Frame = 2 
Query:   80 MSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGK 268
            M Q  +V+ C  C+ FQ Q  +K  KW CK C  KQS+IK +  G   +CR+ VQ+ N   G+
Sbjct:    1 MVQEFNVLRCFSCQTFQVQQVKKAKKWTCKVCGEKQSLIKEFGRGAAADCRRHVQKLNALRGE 63
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Zebrafish
Match: mrnip (MRN complex interacting protein [Source:NCBI gene;Acc:641417])

HSP 1 Score: 51.2174 bits (121), Expect = 3.616e-8
Identity = 26/63 (41.27%), Postives = 36/63 (57.14%), Query Frame = 2 
Query:   80 MSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGK 268
            M Q  +V+ C  C+ FQ Q  +K  KW CK C  KQS+IK +  G   +CR+ VQ+ N   G+
Sbjct:    1 MVQEFNVLRCFSCQTFQVQQVKKAKKWTCKVCGEKQSLIKEFGRGAAADCRRHVQKLNALRGE 63
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Zebrafish
Match: mrnip (MRN complex interacting protein [Source:NCBI gene;Acc:641417])

HSP 1 Score: 51.2174 bits (121), Expect = 3.616e-8
Identity = 26/63 (41.27%), Postives = 36/63 (57.14%), Query Frame = 2 
Query:   80 MSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGK 268
            M Q  +V+ C  C+ FQ Q  +K  KW CK C  KQS+IK +  G   +CR+ VQ+ N   G+
Sbjct:    1 MVQEFNVLRCFSCQTFQVQQVKKAKKWTCKVCGEKQSLIKEFGRGAAADCRRHVQKLNALRGE 63
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Mouse
Match: Mrnip (MRN complex interacting protein [Source:MGI Symbol;Acc:MGI:1915317])

HSP 1 Score: 61.6178 bits (148), Expect = 8.051e-12
Identity = 37/112 (33.04%), Postives = 58/112 (51.79%), Query Frame = 2 
Query:   74 VIMSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGKI-ENNFSHTEEV----------PNESTSINQKIES----SKWNKYLE 364
            +  +Q   V+ C  C IFQ+   +K  KW CK C  KQS ++ Y EG+G +CR+ VQ+ N   G++ E +    EE           P ++ + +Q+  S    S+W KYL+
Sbjct:    1 MAPAQQSRVLRCCSCHIFQAHQVKKSLKWTCKACGEKQSFVRAYGEGSGADCRRHVQKLNLLQGQVSELSLRSVEEAVNGSEEENAGPLQAEAGSQQAPSKPLESRWLKYLD 112
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Mouse
Match: Mrnip (MRN complex interacting protein [Source:MGI Symbol;Acc:MGI:1915317])

HSP 1 Score: 54.6842 bits (130), Expect = 2.479e-10
Identity = 25/58 (43.10%), Postives = 36/58 (62.07%), Query Frame = 2 
Query:   98 VVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGKI 271
            V+ C  C IFQ+   +K  KW CK C  KQS ++ Y EG+G +CR+ VQ+ N   G++
Sbjct:    2 VLRCCSCHIFQAHQVKKSLKWTCKACGEKQSFVRAYGEGSGADCRRHVQKLNLLQGQV 59
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. UniProt/SwissProt
Match: sp|Q6NTE8|MRNIP_HUMAN (MRN complex-interacting protein OS=Homo sapiens OX=9606 GN=MRNIP PE=1 SV=2)

HSP 1 Score: 62.3882 bits (150), Expect = 3.521e-11
Identity = 38/112 (33.93%), Postives = 61/112 (54.46%), Query Frame = 2 
Query:   86 QIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGKI--------ENNFSHTEE---VPNESTSINQKIES----SKWNKYLEDENQ 376
            Q   V+ C  C++FQ+   +K  KW CK C  KQS ++ Y EG+G +CR+ VQ+ N   G++        E   S +EE      ++ ++ Q+ +S    S+W KYLE ++Q
Sbjct:    5 QRSRVLRCCSCRLFQAHQVKKSVKWTCKACGEKQSFLQAYGEGSGADCRRHVQKLNLLQGQVSELPLRSLEETVSASEEENVGHQQAGNVKQQEKSQPSESRWLKYLEKDSQ 116
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. UniProt/SwissProt
Match: sp|Q9D1F5|MRNIP_MOUSE (MRN complex-interacting protein OS=Mus musculus OX=10090 GN=Mrnip PE=2 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 5.632e-11
Identity = 37/112 (33.04%), Postives = 58/112 (51.79%), Query Frame = 2 
Query:   74 VIMSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGKI-ENNFSHTEEV----------PNESTSINQKIES----SKWNKYLE 364
            +  +Q   V+ C  C IFQ+   +K  KW CK C  KQS ++ Y EG+G +CR+ VQ+ N   G++ E +    EE           P ++ + +Q+  S    S+W KYL+
Sbjct:    1 MAPAQQSRVLRCCSCHIFQAHQVKKSLKWTCKACGEKQSFVRAYGEGSGADCRRHVQKLNLLQGQVSELSLRSVEEAVNGSEEENAGPLQAEAGSQQAPSKPLESRWLKYLD 112
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. UniProt/SwissProt
Match: sp|Q3ZBG8|MRNIP_BOVIN (MRN complex-interacting protein OS=Bos taurus OX=9913 GN=MRNIP PE=2 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 5.018e-9
Identity = 38/116 (32.76%), Postives = 59/116 (50.86%), Query Frame = 2 
Query:   74 VIMSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGKIE--------NNFSHTEE---VPNES--TSINQKIESSK--WNKYLEDENQ 376
            +   Q   V+ C  C +FQ+   +K  KW CK C  KQS ++ Y EG+G +CR+ VQ+ N   G+I          + S  EE    P ++   S  + ++ SK  W KYLE +++
Sbjct:    1 MAPPQQARVLRCCCCSLFQAHQVKKSLKWTCKACGKKQSFLQAYGEGSGADCRRHVQKLNLLQGQISEMSLRSLGESISANEEENVGPWQAARASPQETLQPSKNRWLKYLERDSK 116
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. UniProt/SwissProt
Match: sp|Q32PP1|MRNIP_DANRE (MRN complex-interacting protein OS=Danio rerio OX=7955 GN=mrnip PE=2 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 2.507e-7
Identity = 26/62 (41.94%), Postives = 35/62 (56.45%), Query Frame = 2 
Query:   80 MSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALG 265
            M Q  +V+ C  C+ FQ Q  +K  KW CK C  KQS+IK +  G   +CR+ VQ+ N   G
Sbjct:    1 MVQEFNVLRCFSCQTFQVQQVKKAKKWTCKVCGEKQSLIKEFGRGAAADCRRHVQKLNALRG 62
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. TrEMBL
Match: A0A1D2MYL3 (Uncharacterized protein OS=Orchesella cincta OX=48709 GN=Ocin01_08562 PE=4 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 2.825e-14
Identity = 37/68 (54.41%), Postives = 47/68 (69.12%), Query Frame = 2 
Query:   80 MSQIIHVVCCSECKIFQSQI-KRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGKIENN 280
            M QI+HV+ C ECK FQ  I K+  NKW CK C  KQS+ +VY +GTG ECR+ VQ+ N A G++E N
Sbjct:    1 MPQILHVLVCVECKTFQVHIVKKSSNKWNCKICNTKQSVQQVYTKGTGAECREVVQKLNQARGQLEEN 68
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. TrEMBL
Match: A0A026WWJ4 (Uncharacterized protein OS=Ooceraea biroi OX=2015173 GN=DMN91_010812 PE=4 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 2.026e-13
Identity = 41/106 (38.68%), Postives = 60/106 (56.60%), Query Frame = 2 
Query:   80 MSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQ------NNYA-----LGKIENNFSHTEEVPNESTSINQKIESSKWNKYLE 364
            M Q ++V+CC  CK+FQ  I +K  KW CK C  KQS+ ++Y +G+G +CR +VQQ      N+ A          +N+S +  + +ES   N K   SKW KYL+
Sbjct:    1 MPQEMNVLCCYSCKMFQVHIVKKARKWQCKLCNAKQSVQQIYLQGSGRDCRLYVQQLNSLKANDIASLIAERNNTSDNYSASASISHESN--NDKAAESKWAKYLD 104
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. TrEMBL
Match: A0A433T3M2 (Uncharacterized protein (Fragment) OS=Elysia chlorotica OX=188477 GN=EGW08_016081 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.403e-12
Identity = 40/110 (36.36%), Postives = 58/110 (52.73%), Query Frame = 2 
Query:   89 IIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGKIEN--------NFSHTEEV-----PNESTSINQKIES--SKWNKYLEDEN 373
            + HV+ C++C  FQ     K  KW CK C LKQS+IKV+ EGTG ECR  VQ+ N   G+ +         N +  EE      P      ++ ++S  S+W+ YL+D +
Sbjct:    2 VFHVLRCAKCDTFQVLQVTKSKKWTCKLCGLKQSVIKVFGEGTGAECRHHVQKLNTLRGQADQILEEKHLINVTEPEESASQHRPTSPAEPSRVLDSSGSRWSSYLDDRD 111
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. TrEMBL
Match: A0A1S3HWB3 (uncharacterized protein LOC106158770 OS=Lingula unguis OX=7574 GN=LOC106158770 PE=4 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 1.448e-12
Identity = 40/118 (33.90%), Postives = 64/118 (54.24%), Query Frame = 2 
Query:   80 MSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGKIEN----NFSHTEEVPNESTSINQKIES---------------SKWNKYLEDENQ 376
            M Q+ HV+ C  C++FQ    +K NKW CK C  KQS+IKV+ EG+G +CRK VQ+ N    +++     N++   ++ ++    N+ I +               SKW KY+ +E+Q
Sbjct:    1 MPQVFHVLQCFSCRMFQVHQVKKINKWACKLCGQKQSVIKVFGEGSGADCRKHVQKLNMVRSQVDTADQENWTSAAKITSDEVDWNENISTVSEYLQVGSSAGVAESKWGKYVTEEDQ 118
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. TrEMBL
Match: M3XNI0 (MRN complex interacting protein OS=Mustela putorius furo OX=9669 GN=MRNIP PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.342e-12
Identity = 41/116 (35.34%), Postives = 64/116 (55.17%), Query Frame = 2 
Query:   74 VIMSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGKI-ENNFSHTEEVPN------------ESTSINQKIESS--KWNKYLEDENQ 376
            ++  Q + V+ C  C++FQ+  ++K  KW CK C  KQS ++ Y EG+G +CR+ VQ+ N   G+I E  F   EE  N            E  S+ +K +SS  +W KYLE +++
Sbjct:    1 MVPPQRVRVLRCCRCRLFQAHQEKKGLKWTCKACGEKQSFLRTYGEGSGADCRRHVQKLNLLQGQISEMAFRSLEEPGNVDEEENVGPRQAEHESVQEKPQSSENRWLKYLEKDSK 116
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Cavefish
Match: ENSAMXT00000033288.1 (MRN complex interacting protein [Source:NCBI gene;Acc:103032689])

HSP 1 Score: 66.6254 bits (161), Expect = 1.298e-13
Identity = 37/95 (38.95%), Postives = 51/95 (53.68%), Query Frame = 2 
Query:   80 MSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGKIENNFSHTEEVPNESTSINQKIESSKWNKYLE 364
            M Q   V+ C  C+ FQ Q  +K  KW CK C  KQS+IK Y  GTG +CR+ VQ+ N   G +        EV NE     ++   S+W+KY++
Sbjct:    1 MVQEFQVLRCFSCQTFQVQQVKKSKKWTCKMCGEKQSLIKEYGRGTGADCRRHVQKLNSLRGDL-------LEVENERAQDEEQATESRWSKYVD 88
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Cavefish
Match: ENSAMXT00000030226.1 (MRN complex interacting protein [Source:NCBI gene;Acc:103032689])

HSP 1 Score: 58.9214 bits (141), Expect = 7.910e-11
Identity = 39/113 (34.51%), Postives = 57/113 (50.44%), Query Frame = 2 
Query:   80 MSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALG---KIENNFSHTE-----------EVPNESTSINQKIE----SSKWNKYLE 364
            M Q   V+ C  C+ FQ Q  +K  KW CK C  KQS+IK Y  GTG +CR+ VQ+ N   G   ++EN  +  +              +E+ S  Q+ E     S+W+KY++
Sbjct:    1 MVQEFQVLRCFSCQTFQVQQVKKSKKWTCKMCGEKQSLIKEYGRGTGADCRRHVQKLNSLRGDLLEVENERAQAQWKEKKECREEDGFGDEAQSCEQQDEEQATESRWSKYVD 113
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Nematostella
Match: EDO37661 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SF83])

HSP 1 Score: 43.1282 bits (100), Expect = 7.116e-6
Identity = 20/52 (38.46%), Postives = 30/52 (57.69%), Query Frame = 2 
Query:  143 RKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGKIENNFSHTEE 298
            +K  KW CK C  KQSI K++  G+  +CR+ VQ+ N   G+I    +  E+
Sbjct:   14 KKAKKWACKICGEKQSIKKLHARGSAPDCRRHVQKLNMIQGEIAEGLAAKED 65
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. Planmine SMEST
Match: SMESG000034778.1 (SMESG000034778.1)

HSP 1 Score: 201.06 bits (510), Expect = 2.464e-68
Identity = 99/100 (99.00%), Postives = 100/100 (100.00%), Query Frame = 2 
Query:   80 MSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGKIENNFSHTEEVPNESTSINQKIESSKWNKYLEDENQF 379
            MSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGKIENNFSHTEEVPNESTSINQKIESSKWNKYLEDE+QF
Sbjct:    1 MSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFVQQNNYALGKIENNFSHTEEVPNESTSINQKIESSKWNKYLEDEDQF 100
[Close]
BLAST of UPF0544 protein C5orf45 like protein vs. Planmine SMEST
Match: SMESG000034778.1 (SMESG000034778.1)

HSP 1 Score: 102.834 bits (255), Expect = 1.242e-29
Identity = 56/85 (65.88%), Postives = 60/85 (70.59%), Query Frame = 3 
Query:  105 AAPNVKFFNLK*SEKITNGFVKNVF*NKA*LKFILKELDSSVGNLCSKIIMHLVKLKIIFHIQRRFPMNQLQLIRKSSLVNGTNI 359
                 K F  +   K      K  F  ++    I+KELDSSVGNLCSKIIMHLVKLKIIFHIQRRFPMNQLQLIRKSSLVNGTNI
Sbjct:    9 CCSECKIFQSQIKRKDNKWICKKCFLKQS----IIKELDSSVGNLCSKIIMHLVKLKIIFHIQRRFPMNQLQLIRKSSLVNGTNI 89

HSP 2 Score: 86.2705 bits (212), Expect = 4.284e-23
Identity = 43/54 (79.63%), Postives = 44/54 (81.48%), Query Frame = 2 
Query:   80 MSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFECRKFV 241
            MSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIK      G  C K +
Sbjct:    1 MSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKELDSSVGNLCSKII 54
The following BLAST results are available for this feature:
BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MRNIP2.968e-1233.93MRN complex interacting protein [Source:HGNC Symbo... [more]
MRNIP2.968e-1233.93MRN complex interacting protein [Source:HGNC Symbo... [more]
MRNIP5.112e-1233.93MRN complex interacting protein [Source:HGNC Symbo... [more]
MRNIP5.112e-1233.93MRN complex interacting protein [Source:HGNC Symbo... [more]
MRNIP7.332e-1233.93MRN complex interacting protein [Source:HGNC Symbo... [more]
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BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG432953.261e-1045.16gene:FBgn0262987 transcript:FBtr0306825[more]
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BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
mrnip3.616e-841.27MRN complex interacting protein [Source:NCBI gene;... [more]
mrnip3.616e-841.27MRN complex interacting protein [Source:NCBI gene;... [more]
mrnip3.616e-841.27MRN complex interacting protein [Source:NCBI gene;... [more]
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BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Mrnip8.051e-1233.04MRN complex interacting protein [Source:MGI Symbol... [more]
Mrnip2.479e-1043.10MRN complex interacting protein [Source:MGI Symbol... [more]
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BLAST of UPF0544 protein C5orf45 like protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|Q6NTE8|MRNIP_HUMAN3.521e-1133.93MRN complex-interacting protein OS=Homo sapiens OX... [more]
sp|Q9D1F5|MRNIP_MOUSE5.632e-1133.04MRN complex-interacting protein OS=Mus musculus OX... [more]
sp|Q3ZBG8|MRNIP_BOVIN5.018e-932.76MRN complex-interacting protein OS=Bos taurus OX=9... [more]
sp|Q32PP1|MRNIP_DANRE2.507e-741.94MRN complex-interacting protein OS=Danio rerio OX=... [more]
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BLAST of UPF0544 protein C5orf45 like protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1D2MYL32.825e-1454.41Uncharacterized protein OS=Orchesella cincta OX=48... [more]
A0A026WWJ42.026e-1338.68Uncharacterized protein OS=Ooceraea biroi OX=20151... [more]
A0A433T3M21.403e-1236.36Uncharacterized protein (Fragment) OS=Elysia chlor... [more]
A0A1S3HWB31.448e-1233.90uncharacterized protein LOC106158770 OS=Lingula un... [more]
M3XNI02.342e-1235.34MRN complex interacting protein OS=Mustela putoriu... [more]
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BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSAMXT00000033288.11.298e-1338.95MRN complex interacting protein [Source:NCBI gene;... [more]
ENSAMXT00000030226.17.910e-1134.51MRN complex interacting protein [Source:NCBI gene;... [more]
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BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO376617.116e-638.46Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of UPF0544 protein C5orf45 like protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of UPF0544 protein C5orf45 like protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000034778.12.464e-6899.00SMESG000034778.1[more]
SMESG000034778.11.242e-2965.88SMESG000034778.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027476 ID=SMED30027476|Name=UPF0544 protein C5orf45 like protein|organism=Schmidtea mediterranea sexual|type=transcript|length=390bp
GAAAAATTAAATCAAGAAAATCAAGAAACTCAATAATTTTTGAAGTTTCA
AAATTTTAGTATATAAATAGTGTGTTATAATGTCTCAAATTATTCATGTA
GTATGCTGCTCCGAATGTAAAATTTTTCAATCTCAAATAAAGCGAAAAGA
TAACAAATGGATTTGTAAAAAATGTTTTTTGAAACAAAGCATAATTAAAG
TTTATTTTGAAGGAACTGGATTCGAGTGTAGGAAATTTGTGCAGCAAAAT
AATTATGCACTTGGTAAAATTGAAAATAATTTTTCACATACAGAGGAGGT
TCCCAATGAATCAACTTCAATTAATCAGAAAATCGAGTCTAGTAAATGGA
ACAAATATTTAGAGGATGAAAATCAATTCTGATTTAATGT
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protein sequence of SMED30027476-orf-1

>SMED30027476-orf-1 ID=SMED30027476-orf-1|Name=SMED30027476-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=101bp
MSQIIHVVCCSECKIFQSQIKRKDNKWICKKCFLKQSIIKVYFEGTGFEC
RKFVQQNNYALGKIENNFSHTEEVPNESTSINQKIESSKWNKYLEDENQF
*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR032739MRNIP
Vocabulary: biological process
TermDefinition
GO:0006886intracellular protein transport
GO:0006888ER to Golgi vesicle-mediated transport
Vocabulary: molecular function
TermDefinition
GO:0008270zinc ion binding
Vocabulary: cellular component
TermDefinition
GO:0030127COPII vesicle coat
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032739MRN complex-interacting proteinPFAMPF15749MRNIPcoord: 7..92
e-value: 1.1E-18
score: 67.9
IPR032739MRN complex-interacting proteinPANTHERPTHR15863FAMILY NOT NAMEDcoord: 1..98