FMRFamide-activated amiloride-sensitive sodium channel

Overview
NameFMRFamide-activated amiloride-sensitive sodium channel
Smed IDSMED30027466
Length (bp)1795
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of FMRFamide-activated amiloride-sensitive sodium channel (SMED30027466) t-SNE clustered cells

Violin plots show distribution of expression levels for FMRFamide-activated amiloride-sensitive sodium channel (SMED30027466) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of FMRFamide-activated amiloride-sensitive sodium channel (SMED30027466) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for FMRFamide-activated amiloride-sensitive sodium channel (SMED30027466) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30027466

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30027466SMESG000034894.1 dd_Smed_v4_15920_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30027466SMESG000034894.1 dd_Smed_v4_15920_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30027466SMESG000034894.1 dd_Smed_v4_15920_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30027466SMESG000034894.1 dd_Smed_v4_15920_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Human
Match: SCNN1B (sodium channel epithelial 1 beta subunit [Source:HGNC Symbol;Acc:HGNC:10600])

HSP 1 Score: 53.1434 bits (126), Expect = 1.178e-6
Identity = 46/187 (24.60%), Postives = 77/187 (41.18%), Query Frame = 3 
Query:  801 EKSFIPGILNQLSGLRGMIHERFTFPDIENEGFHLEPGKLVEINYAVIKKSKIATPKQPCS---KEVREKIIDLDKSFVFSQSACFRSSKGWSSLEKCSC-FFADTMRMGIPNVTHPYCGNILNISFGMLMKRVECLSDWSSEFNKQIKEIFNNKCKSSCVHFQYDLSLSVTKWLPTNWQLGWLIDI 1349
            ++ ++P  L   +G+R M+HE+ ++P I +EG +   G    I   V K  ++  P  PC+    EV  +    D +  +S  AC RS      +  C+C  +   +  G       YC N              C SD      +  +E     CK SC   QY +++S+  W P+     W+  +
Sbjct:  303 QEDYVP-FLASTAGVRLMLHEQRSYPFIRDEGIYAMSGTETSIGVLVDKLQRMGEPYSPCTVNGSEVPVQNFYSDYNTTYSIQACLRSCFQDHMIRNCNCGHYLYPLPRG-----EKYCNN------RDFPDWAHCYSDLQMSVAQ--RETCIGMCKESCNDTQYKMTISMADW-PSEASEDWIFHV 474
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Human
Match: SCNN1B (sodium channel epithelial 1 beta subunit [Source:HGNC Symbol;Acc:HGNC:10600])

HSP 1 Score: 53.1434 bits (126), Expect = 1.237e-6
Identity = 46/187 (24.60%), Postives = 77/187 (41.18%), Query Frame = 3 
Query:  801 EKSFIPGILNQLSGLRGMIHERFTFPDIENEGFHLEPGKLVEINYAVIKKSKIATPKQPCS---KEVREKIIDLDKSFVFSQSACFRSSKGWSSLEKCSC-FFADTMRMGIPNVTHPYCGNILNISFGMLMKRVECLSDWSSEFNKQIKEIFNNKCKSSCVHFQYDLSLSVTKWLPTNWQLGWLIDI 1349
            ++ ++P  L   +G+R M+HE+ ++P I +EG +   G    I   V K  ++  P  PC+    EV  +    D +  +S  AC RS      +  C+C  +   +  G       YC N              C SD      +  +E     CK SC   QY +++S+  W P+     W+  +
Sbjct:  276 QEDYVP-FLASTAGVRLMLHEQRSYPFIRDEGIYAMSGTETSIGVLVDKLQRMGEPYSPCTVNGSEVPVQNFYSDYNTTYSIQACLRSCFQDHMIRNCNCGHYLYPLPRG-----EKYCNN------RDFPDWAHCYSDLQMSVAQ--RETCIGMCKESCNDTQYKMTISMADW-PSEASEDWIFHV 447
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Human
Match: SCNN1B (sodium channel epithelial 1 beta subunit [Source:HGNC Symbol;Acc:HGNC:10600])

HSP 1 Score: 53.1434 bits (126), Expect = 1.346e-6
Identity = 46/187 (24.60%), Postives = 77/187 (41.18%), Query Frame = 3 
Query:  801 EKSFIPGILNQLSGLRGMIHERFTFPDIENEGFHLEPGKLVEINYAVIKKSKIATPKQPCS---KEVREKIIDLDKSFVFSQSACFRSSKGWSSLEKCSC-FFADTMRMGIPNVTHPYCGNILNISFGMLMKRVECLSDWSSEFNKQIKEIFNNKCKSSCVHFQYDLSLSVTKWLPTNWQLGWLIDI 1349
            ++ ++P  L   +G+R M+HE+ ++P I +EG +   G    I   V K  ++  P  PC+    EV  +    D +  +S  AC RS      +  C+C  +   +  G       YC N              C SD      +  +E     CK SC   QY +++S+  W P+     W+  +
Sbjct:  348 QEDYVP-FLASTAGVRLMLHEQRSYPFIRDEGIYAMSGTETSIGVLVDKLQRMGEPYSPCTVNGSEVPVQNFYSDYNTTYSIQACLRSCFQDHMIRNCNCGHYLYPLPRG-----EKYCNN------RDFPDWAHCYSDLQMSVAQ--RETCIGMCKESCNDTQYKMTISMADW-PSEASEDWIFHV 519
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Mouse
Match: Scnn1b (sodium channel, nonvoltage-gated 1 beta [Source:MGI Symbol;Acc:MGI:104696])

HSP 1 Score: 50.0618 bits (118), Expect = 8.787e-6
Identity = 65/284 (22.89%), Postives = 107/284 (37.68%), Query Frame = 3 
Query:  543 YQHLSPNDADNLSWDNETFIICSIFRGSTMEVGLGTNCSMHINKISI--PQYFNCF----SVTPDDSDPQSRITSITLLIRVSGGMNDTVEHEKSFIPGILNQLSGLRGMIHERFTFPDIENEGFHLEPGKLVEINYAVIKKSKIATPKQPCS---KEVREKIIDLDKSFVFSQSACFRSSKGWSSLEKCSC-FFADTMRMGIPNVTHPYCGNILNISFGMLMKRVECLSDWSSEF-NKQI----KEIFNNKCKSSCVHFQYDLSLSVTKWLPTNWQLGWLIDI 1349
            +  + P D   + +  +  I+  +F         GT    H N   I  P Y NC+     +T +     +  T   L + +  G  D       ++P  L   +G R M+HE+ T+P I  EG +   G    I   V K  +   P  PC+    +V  K +    +  +S  AC  S      +  CSC  +   +  G       YC N                 DW+  + N Q+    +E   + CK SC   QY +++S+  W P+     W++ +
Sbjct:  225 FSQVLPQDLVGMGYAPDRIILACLF---------GTEPCSHRNFTPIFYPDYGNCYIFNWGMTEETLPSANPGTEFGLKLILDIGQED-------YVP-FLASTAGARLMLHEQRTYPFIREEGIYAMAGTETSIGVLVDKLQRKGEPYSPCTMNGSDVAIKNLYSVYNTTYSIQACLHSCFQDHMIRNCSCGHYLYPLPEG-----EKYCNN-------------RDFPDWAYCYLNLQMSVTQRETCLSMCKESCNDTQYKMTISMADW-PSEASEDWILHV 472
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. UniProt/SwissProt
Match: sp|Q92075|SCNNA_CHICK (Amiloride-sensitive sodium channel subunit alpha OS=Gallus gallus OX=9031 GN=SCNN1A PE=2 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 2.302e-6
Identity = 56/252 (22.22%), Postives = 110/252 (43.65%), Query Frame = 3 
Query:  558 PNDADNLSWDNETFIICSIFRGSTMEVGLGTNCSMHINKISIPQYFNCFSVTPDDSDPQSRITSITLLIRVSGGMNDTVEHEKS-FIPGILNQLSGLRGMIHERFTFPDIENEGFHLEPGKLVEINYAVIKKSKIATPKQPCSKEVREKIIDLDKSFVFSQSACFRSSKGWSSLEKCSCFFADTMRMGIPNVTHPYCGNILNISFGMLMKRVECLSDWSSEFNKQIKEIFNNKCKSSCVHFQYDLSLSVTKW 1310
            P+  D    D E FI    F  +T +         +      P Y NC++   + S   S  TS   L  ++ G++  V  E++ FIP +L+ ++G R M+H++     +++ GF++ PG    I+       ++      C+++  +  +    S  +++  C RS    + +++CSC +       +P+    YC    ++++G       C     +EF   +   F +KC+  C   +Y LS   ++W
Sbjct:  252 PDAKDLDESDFENFIYACRFNEATCD-------KANYTHFHHPLYGNCYTFNDNSS---SLWTSS--LPGINNGLSLVVRTEQNDFIP-LLSTVTGARVMVHDQNEPAFMDDGGFNVRPGIETSISMRKEMTERLGGSYSDCTEDGSDVPVQNLYSSRYTEQVCIRSCFQLNMVKRCSCAY---YFYPLPDGAE-YCDYTKHVAWGY------CYYKLLAEFKADVLGCF-HKCRKPCKMTEYQLSAGYSRW 479
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. UniProt/SwissProt
Match: sp|P51168|SCNNB_HUMAN (Amiloride-sensitive sodium channel subunit beta OS=Homo sapiens OX=9606 GN=SCNN1B PE=1 SV=2)

HSP 1 Score: 53.1434 bits (126), Expect = 5.657e-6
Identity = 46/187 (24.60%), Postives = 77/187 (41.18%), Query Frame = 3 
Query:  801 EKSFIPGILNQLSGLRGMIHERFTFPDIENEGFHLEPGKLVEINYAVIKKSKIATPKQPCS---KEVREKIIDLDKSFVFSQSACFRSSKGWSSLEKCSC-FFADTMRMGIPNVTHPYCGNILNISFGMLMKRVECLSDWSSEFNKQIKEIFNNKCKSSCVHFQYDLSLSVTKWLPTNWQLGWLIDI 1349
            ++ ++P  L   +G+R M+HE+ ++P I +EG +   G    I   V K  ++  P  PC+    EV  +    D +  +S  AC RS      +  C+C  +   +  G       YC N              C SD      +  +E     CK SC   QY +++S+  W P+     W+  +
Sbjct:  303 QEDYVP-FLASTAGVRLMLHEQRSYPFIRDEGIYAMSGTETSIGVLVDKLQRMGEPYSPCTVNGSEVPVQNFYSDYNTTYSIQACLRSCFQDHMIRNCNCGHYLYPLPRG-----EKYCNN------RDFPDWAHCYSDLQMSVAQ--RETCIGMCKESCNDTQYKMTISMADW-PSEASEDWIFHV 474
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. TrEMBL
Match: A0A4S2JQK4 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_011292 PE=3 SV=1)

HSP 1 Score: 243.432 bits (620), Expect = 1.381e-68
Identity = 178/546 (32.60%), Postives = 284/546 (52.01%), Query Frame = 3 
Query:  147 LKTLICKTLSIRGIGKIGSSQDRFIQFIWTIFVLSMSILLTYATVQLVQEFLNYDLVIRISIKSNDYSTEXXXXXXXXXXXXGSRAKRYWDENLIMSPTKFNRKIRDQMK-HIMENNKGSPYNLFTKDERRIYYQHLSPNDADNLSWDNETFIICSIFRGSTMEVGLGTNCS----MHINKISIPQYFNCFSVTPDDSDPQSRITSITLLIRVSGGMNDTVEHEKSFIPGILNQLSGLRGMIHERFTFPDIENEGFHLEPGKLVEINYAVIKKSKIATPKQPCSKEVREKI-----------------------IDLDKSFVFSQSACFRSSKGWSSLEKCSCFFADTMRMGIPNVTHPYCGNIL--NISFGMLMKRVECLSDWSSEFNKQIKEIF-NNKCKSSCVHFQYDLSLSVTKWLPTNWQLGWLIDIDAAGKGFQPDSQQX-----XXXXXXXXXXXXYINSQDLFNYP-NSGKLSYIDKDNFVMVVIKRRNFDTMEHREKLTLDLYNLISRFXXXXXXXXXXXXAFLLEIVEMIYLAAED 1673
            L  L+  T +IRG+ ++     RF++ +W  FV++M++ L   +  LVQ++L YD+ + + + + D  + FP+ + CHHHPF + A R W EN ++SP+ FNR++R   + H+  NN     NL+  D   IYYQ+L+  DA  L  D   F+ C   R     + +  +C+      I K S   +FNC ++ P D        +I L++ + G   D  + E++F+  +     GLR ++HE   +PD+E EG H+EPGKL EINY  I   ++ TP+ PC  E  ++                         DL+ S+ ++QS C       + ++KC C +    R   P+   PYCG+ L        L KR+ECL+  +   N  +K I    KC   C ++ Y+ ++SVT W   NWQL WL  ++ A +  Q + +++             +++ Y++SQ+L N P N  K   ++  NF  VV++RR+ D   ++EKL L +  L+SR GGLCSL +GLT AF++E+VE  YL   D
Sbjct:    8 LIKLVSDTFTIRGVPRLNRGP-RFLRGLWWFFVITMTVGLFLTSYYLVQDYLLYDVAVNVHV-ALDAHSPFPALTVCHHHPFSAEAYRLWRENKVLSPSAFNREMRKLTQYHLERNNLDISSNLYQYDSMSIYYQNLNTEDAYRLGHDTSIFLNC--MRRVNQAMQIEDDCTKMDGFRIRKFSHHTFFNCHTIEPTDKMEAEATDTIALVVGL-GPTPDYSDEEQAFLMDLYEMAQGLRVVVHEPGAYPDLEREGLHVEPGKLNEINYQPIHWQRLNTPRHPCRTERVDRTHELASITSDRLPKSPSSDYAYAYTDLNVSYRYTQSQCIHLYMQLNIIDKCHCQYIYNPRPKHPSKEVPYCGSFLYEQDKTLALSKRIECLN--AGPMNTSLKRIHAATKCFPLCNYYSYESTMSVTTWRTENWQLHWLRHLNNAFQAMQREQEEKGPDFNVTKSAGYARWKEYLDSQNLTNIPTNDLKKLNLEGSNFAYVVLRRRSQDLQINKEKLVLSISVLLSRIGGLCSLTIGLTAAFVVELVEFTYLLCTD 546
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. TrEMBL
Match: A0A267EKX1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig014550g1 PE=3 SV=1)

HSP 1 Score: 236.113 bits (601), Expect = 2.328e-66
Identity = 160/523 (30.59%), Postives = 260/523 (49.71%), Query Frame = 3 
Query:  156 LICKTLSIRGIGKIGSSQDRFIQFIWTIFVLSMSILLTYATVQLVQEFLNYDLVIRISIKSNDYSTEXXXXXXXXXXXXGSRAKRYWDENLIMSPTKFNRKIRDQ-MKHIMENNKGSPYNLFTKDERRIYYQHLSPNDADNLSWD-NETFIICSIFRGSTMEVGLGTNCSMHINKISIPQYFNCFSVTPDDSDPQSRITSITLLIRV--SGGMNDTVEHEKSFIPGILNQLSGLRGMIHERFTFPDIENEGFHLEPGKLVEINYAVIKKSKIATPKQPCSKEV---REKIIDLDKSFVFSQSACFRSSKGWSSLEKCSCFFADTMRMGIPNVTHPYCGNILNISFGMLMKRVECLSDWSSEFNKQIKEIFNNKCKSSCVHFQYDLSLSVTKWLPTNWQLGWLI-------DIDAAGKGF-------QPDSQQXXXXXXXXXXXXXYINSQDLFNYPNSGKLSYIDKDNFVMVVIKRRNFDTMEHREKLTLDLYNLISRFXXXXXXXXXXXXAFLLEIVEMIYL 1661
            L C   S+RG+ K   +  R ++ IWT +V +M+  L      +V ++L+Y  VI+ ++  ND    +P+ + C H+PF    ++ W    I+SPT F+R +R   +K     N      +   D +R+YYQ+L P+DA+ LS    + FI C    GS ME+  G  C+M +  IS+P YFNCF V    +     I S+T+++RV  S   +    HE++F+     Q SG R  + E   FP+  + G H+ PGK+VE+ Y  ++  ++ +P   C  E      +++DL + F +SQ  C R   G   L+ C C FAD +R   PN THPYCG+      G +++   CL   S         + +  C   C +  +++  S T W   +WQL WL        D+  A +         +   Q E + +  K    +++ +++L        L    +D F  + ++R+  +T++  E L+L    L+S+ GGL SL LGLTCAF +E++E+  L
Sbjct:    9 LFCTATSLRGVAKTVKAPTRPLRCIWTAYVAAMAAFLVTCVCFIVTDYLSYRTVIQTTVVLNDPGLSWPAVTVCGHYPFTESGRQLWQSGQILSPTNFSRMLRAHALKMFDAGNHIGAEEILASDSKRVYYQNLEPDDAEKLSHTAQQLFIFCINATGSFMEMSSGDGCAMQLKLISLPDYFNCFQVQATPT-----IDSLTVVLRVTDSHHQHGADPHEQAFVQDAFQQASGFRVQLQEHDRFPEPTDRGIHVAPGKMVEMAYQPVRLIEVGSPTDACLPETIASSRRVLDLGRDFAYSQEGCLRQELGRIVLKACGCHFADLLRETPPNATHPYCGDYRR-GVGPVLRSRACLQ--SLNLPSLRANLGDTHCLKPCHYHDFEMVSSKTIWRAADWQLHWLALQAKSFRDLAEASERLADLTASSEQRRQAEASIQSAKNSLRDFLTAKNLTAISGPQVLEPSGRD-FAYITVRRQTRNTIQKTETLSLSFSALMSQVGGLSSLTLGLTCAFAVELLELCLL 522
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. TrEMBL
Match: A0A1I8HS76 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 234.187 bits (596), Expect = 1.191e-65
Identity = 161/523 (30.78%), Postives = 259/523 (49.52%), Query Frame = 3 
Query:  156 LICKTLSIRGIGKIGSSQDRFIQFIWTIFVLSMSILLTYATVQLVQEFLNYDLVIRISIKSNDYSTEXXXXXXXXXXXXGSRAKRYWDENLIMSPTKFNRKIRDQ-MKHIMENNKGSPYNLFTKDERRIYYQHLSPNDADNLSWD-NETFIICSIFRGSTMEVGLGTNCSMHINKISIPQYFNCFSVTPDDSDPQSRITSITLLIRV--SGGMNDTVEHEKSFIPGILNQLSGLRGMIHERFTFPDIENEGFHLEPGKLVEINYAVIKKSKIATPKQPCSKEV---REKIIDLDKSFVFSQSACFRSSKGWSSLEKCSCFFADTMRMGIPNVTHPYCGNILNISFGMLMKRVECLSDWSSEFNKQIKEIFNNKCKSSCVHFQYDLSLSVTKWLPTNWQLGWLI-------DIDAAGKGF-------QPDSQQXXXXXXXXXXXXXYINSQDLFNYPNSGKLSYIDKDNFVMVVIKRRNFDTMEHREKLTLDLYNLISRFXXXXXXXXXXXXAFLLEIVEMIYL 1661
            L C   S+RG+ K   +  R ++ IWT +V +M+  L      +V ++L+Y  VI+ ++  ND    +P+ + C H+PF    ++ W    I+SPT F+R +R   +K     N      +   D +R+YYQ+L P+DA+ LS    + FI C    GS ME+  G  C+M +  IS+P YFNCF V    +     I S+T+++RV  S   +    HE++F+     Q SG R  + E   FP+  + G H+ PGK+VE+ Y  ++  ++ +P   C  E      +++DL + F +SQ  C R   G   L+ C C FAD +R   PN THPYCG+       +L  R  CL   S         + +  C   C +  +++  S T W   +WQL WL        D+  A +         +   Q E + +  K    +++ +++L        L    +D F  + ++R+  +T++  E L+L    L+S+ GGL SL LGLTCAF +E++E+  L
Sbjct:    9 LFCTATSLRGVAKTVKAPTRPLRCIWTAYVAAMAAFLVTCVCFIVTDYLSYKTVIQTTVVLNDPGLSWPAVTVCGHYPFTESGRQLWQSGQILSPTNFSRMLRAHALKMFDAGNHIGAEEILASDSKRVYYQNLEPDDAEKLSHTAQQLFIFCINATGSFMEMSSGDGCAMQLKLISLPDYFNCFQVQATPT-----IDSLTVVLRVTDSHHQHGADPHEQAFVQDAFQQASGFRVQLQEHDRFPEPTDRGIHVAPGKMVEMAYQPVRLIEVGSPTDACLPETIASSRRVLDLGRDFAYSQEGCLRQELGRIVLKACGCHFADLLRETPPNATHPYCGDYRRGVEPVLRSRA-CLQ--SLNLPSLRANLGDTHCLKPCHYHDFEMVSSKTIWRAADWQLHWLALQAKSFRDLAEASERLADLTASSEQRRQAEASIQSAKNSLRDFLTAKNLTAISGPQVLEPSGRD-FAYITVRRQTRNTIQKTETLSLSFSALMSQVGGLSSLTLGLTCAFAVELLELCLL 522
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. TrEMBL
Match: A0A1I8G5D0 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Macrostomum lignano OX=282301 PE=3 SV=1)

HSP 1 Score: 244.588 bits (623), Expect = 1.225e-65
Identity = 162/531 (30.51%), Postives = 285/531 (53.67%), Query Frame = 3 
Query:  147 LKTLICKTLSIRGIGKIGSSQDRFIQFIWTIFVLSMSILLTYATVQLVQEFLNYDLVIRISIKSNDYSTEXXXXXXXXXXXXGSRAKRYWDE-NLIMSPTKFNRKIRDQMKHIM-ENNKGSPYNLFTKDERRIYYQHLSPNDADNLSWDNETFII-CSIFRGSTMEVGLGTNCSMHINKISIPQYFNCFSVTPDDSDPQSRITSITLLIRVSGGMNDTVEH--EKSFIPGILNQLSGLRGMIHERFTFPDIENEGFHLEPGKLVEINY-AVIKKSKIATPKQPCSKEVREKIIDLDKSFVFSQSACFRSSKGWSSLEKCSCFFADTMR-MGIPNVTHPYCGNILNISFGMLMKRVECLSDWSSEFNKQIKEIF---------NNKCKSSCVHFQYDLSLSVTKWLPTNWQLGWLIDIDAAGKGFQPDSQQXXXXXXXXXXXXXYINSQDLFNYPNS---------GKLSYIDKDNFVMVVIKRRNFDTMEHREKLTLDLYNLISRFXXXXXXXXXXXXAFLLEIVEMIYLA 1664
            ++ L C + S+RG+ KI  +   F++ +W +FVL+M ILL Y++ +++  +  YD+ ++ S+  ND S +FP+ + C H+PF  RA R W + +  ++P  FNR++R   + ++   N      L   D+   YYQ+L PN   ++    + F++ C I  G+ + VG  + C +    +S P+YFNC++V P  +D    ++++ L++ +    +++ +H  E++F+  + +Q  GLR ++HE   +P IEN G H+EPGK+VEI +  V+ K    +P   C    R  I DL   + +S  AC  +      + KC C  +   R  G+P+   PYCG+    +  ++  R+ CL +     +K  +            +++C+  C  + YD  LS+T+W  +++QL W++    + K  + +   E+      +   +Y+ + +L   P S           L+   +D +  VV++R++FDT++  EKLT+    L+SR GGLCSL LGLT AF +EIVE +YLA
Sbjct: 1322 IRQLFCSSSSLRGVPKICRADTTFLRILWLVFVLAMFILLVYSSTRVLMNYREYDVSVQTSVIGNDPS-DFPAITICSHYPFSPRAARMWQQCDGQLAPATFNRRLRQLTRQLLFSGNLRLANTLLFYDKVNTYYQNLDPNVTVSMGHSVDEFLLHCMIAIGNYVMVGKASECQLTFQLVSHPKYFNCYTVKPTQADKSQAMSAVYLIVNLG---SNSRQHPWEQAFVMDVFDQAYGLRLLLHEANLYPGIENAGVHIEPGKMVEIAFDTVLWKETQDSPMDRCHDGWRPAIEDLQNRYRYSYQACKEAHMNKLFIAKCQCLLSRFPRPAGLPSNQIPYCGDFRTSNISIIESRLRCLEEIGEHQSKMPQPAAAAAAAAVADDSQCRKRCTRYSYDQELSITRWRASSYQLHWMLHQSDSWKRLR-EVNNESEKRHLLRNILDYLGADNLTRLPVSYQQQERQQQATLTDSAEDTYAYVVVRRKSFDTLKREEKLTVTFEALMSRVGGLCSLYLGLTMAFFVEIVEFLYLA 1847
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. TrEMBL
Match: A0A267F160 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig022441g1 PE=3 SV=1)

HSP 1 Score: 234.187 bits (596), Expect = 1.377e-65
Identity = 161/523 (30.78%), Postives = 260/523 (49.71%), Query Frame = 3 
Query:  156 LICKTLSIRGIGKIGSSQDRFIQFIWTIFVLSMSILLTYATVQLVQEFLNYDLVIRISIKSNDYSTEXXXXXXXXXXXXGSRAKRYWDENLIMSPTKFNRKIRDQ-MKHIMENNKGSPYNLFTKDERRIYYQHLSPNDADNLSWD-NETFIICSIFRGSTMEVGLGTNCSMHINKISIPQYFNCFSVTPDDSDPQSRITSITLLIRV--SGGMNDTVEHEKSFIPGILNQLSGLRGMIHERFTFPDIENEGFHLEPGKLVEINYAVIKKSKIATPKQPCSKE---VREKIIDLDKSFVFSQSACFRSSKGWSSLEKCSCFFADTMRMGIPNVTHPYCGNILNISFGMLMKRVECLSDWSSEFNKQIKEIFNNKCKSSCVHFQYDLSLSVTKWLPTNWQLGWLI-------DIDAAGKGF-------QPDSQQXXXXXXXXXXXXXYINSQDLFNYPNSGKLSYIDKDNFVMVVIKRRNFDTMEHREKLTLDLYNLISRFXXXXXXXXXXXXAFLLEIVEMIYL 1661
            L C   S+RG+ K   +  R ++ IWT +V +M+  L      +V ++L+Y  VI+ ++  ND    +P+ + C H+PF    ++ W    I+SPT F+R +R   +K     N      +   D +R+YYQ+L P+DA+ LS    + FI C    GS ME+  G  C+M +  IS+P YFNCF V    +     I S+T+++RV  S   +    HE++F+     Q SG R  + E   FP+  + G H+ PGK+VE+ Y  ++  ++ +P   C  E   +  +++DL + F +SQ  C R   G   L+ C C FAD +R   PN THPYCG+       +L  R  CL   S         + +  C   C +  +++  S T W   +WQL WL        D+  A +         +   Q E + +  K    +++ +++L        L    +D F  + ++R+  +T++  E L+L    L+S+ GGL SL LGLTCAF +E++E+  L
Sbjct:    9 LFCTATSLRGVAKTVKAPTRPLRCIWTAYVAAMAAFLVTCVCFIVTDYLSYRTVIQTTVVLNDPGLSWPAVTVCGHYPFTESGRQLWQSGQILSPTNFSRMLRAHALKMFDAGNHIGAEEILASDSKRVYYQNLEPDDAEKLSHTAQQLFIFCINATGSFMEMSSGDGCAMQLKLISLPDYFNCFQVQATPT-----IDSLTVVLRVTDSHHQHGADPHEQAFVQDAFQQASGFRVQLQEHDRFPEPTDRGIHVAPGKMVEMAYQPVRLIEVGSPTDACLPETIALSRRVLDLGRDFAYSQEGCLRQELGRIVLKACGCHFADLLRETPPNATHPYCGDYRRGVEPVLRSRA-CLQ--SLNLPSLRANLGDTHCLKPCHYHDFEMVSSKTIWRAADWQLHWLALQAKSFRDLAEASERLADLTASSEQRRQAEASIQSAKNSLRDFLTAKNLTAISGPQVLEPSGRD-FAYITVRRQTRNTIQKTETLSLSFSALMSQVGGLSSLTLGLTCAFAVELLELCLL 522
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Planmine SMEST
Match: SMESG000034894.1 (SMESG000034894.1)

HSP 1 Score: 1025.77 bits (2651), Expect = 0.000e+0
Identity = 550/550 (100.00%), Postives = 550/550 (100.00%), Query Frame = 3 
Query:  138 MGYLKTLICKTLSIRGIGKIGSSQDRFIQFIWTIFVLSMSILLTYATVQLVQEFLNYDLVIRISIKSNDYSTEXXXXXXXXXXXXGSRAKRYWDENLIMSPTKFNRKIRDQMKHIMENNKGSPYNLFTKDERRIYYQHLSPNDADNLSWDNETFIICSIFRGSTMEVGLGTNCSMHINKISIPQYFNCFSVTPDDSDPQSRITSITLLIRVSGGMNDTVEHEKSFIPGILNQLSGLRGMIHERFTFPDIENEGFHLEPGKLVEINYAVIKKSKIATPKQPCSKEVREKIIDLDKSFVFSQSACFRSSKGWSSLEKCSCFFADTMRMGIPNVTHPYCGNILNISFGMLMKRVECLSDWSSEFNKQIKEIFNNKCKSSCVHFQYDLSLSVTKWLPTNWQLGWLIDIDAAGKGFQPDSQQXXXXXXXXXXXXXYINSQDLFNYPNSGKLSYIDKDNFVMVVIKRRNFDTMEHREKLTLDLYNLISRFXXXXXXXXXXXXAFLLEIVEMIYLAAEDHNXXXXXXXXIIHSEELEIDGKVLDKTRSESPIWLSSL 1787
            MGYLKTLICKTLSIRGIGKIGSSQDRFIQFIWTIFVLSMSILLTYATVQLVQEFLNYDLVIRISIKSNDYSTEFPSFSFCHHHPFGSRAKRYWDENLIMSPTKFNRKIRDQMKHIMENNKGSPYNLFTKDERRIYYQHLSPNDADNLSWDNETFIICSIFRGSTMEVGLGTNCSMHINKISIPQYFNCFSVTPDDSDPQSRITSITLLIRVSGGMNDTVEHEKSFIPGILNQLSGLRGMIHERFTFPDIENEGFHLEPGKLVEINYAVIKKSKIATPKQPCSKEVREKIIDLDKSFVFSQSACFRSSKGWSSLEKCSCFFADTMRMGIPNVTHPYCGNILNISFGMLMKRVECLSDWSSEFNKQIKEIFNNKCKSSCVHFQYDLSLSVTKWLPTNWQLGWLIDIDAAGKGFQPDSQQENANEEFKKKFENYINSQDLFNYPNSGKLSYIDKDNFVMVVIKRRNFDTMEHREKLTLDLYNLISRFGGLCSLCLGLTCAFLLEIVEMIYLAAEDHNKKKKEKRKIIHSEELEIDGKVLDKTRSESPIWLSSL
Sbjct:    1 MGYLKTLICKTLSIRGIGKIGSSQDRFIQFIWTIFVLSMSILLTYATVQLVQEFLNYDLVIRISIKSNDYSTEFPSFSFCHHHPFGSRAKRYWDENLIMSPTKFNRKIRDQMKHIMENNKGSPYNLFTKDERRIYYQHLSPNDADNLSWDNETFIICSIFRGSTMEVGLGTNCSMHINKISIPQYFNCFSVTPDDSDPQSRITSITLLIRVSGGMNDTVEHEKSFIPGILNQLSGLRGMIHERFTFPDIENEGFHLEPGKLVEINYAVIKKSKIATPKQPCSKEVREKIIDLDKSFVFSQSACFRSSKGWSSLEKCSCFFADTMRMGIPNVTHPYCGNILNISFGMLMKRVECLSDWSSEFNKQIKEIFNNKCKSSCVHFQYDLSLSVTKWLPTNWQLGWLIDIDAAGKGFQPDSQQENANEEFKKKFENYINSQDLFNYPNSGKLSYIDKDNFVMVVIKRRNFDTMEHREKLTLDLYNLISRFGGLCSLCLGLTCAFLLEIVEMIYLAAEDHNKKKKEKRKIIHSEELEIDGKVLDKTRSESPIWLSSL 550
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Planmine SMEST
Match: SMESG000060702.1 (SMESG000060702.1)

HSP 1 Score: 226.098 bits (575), Expect = 7.881e-66
Identity = 162/522 (31.03%), Postives = 270/522 (51.72%), Query Frame = 3 
Query:  123 QKLIAMGYLKTLICKTLSIRGIGKIGSSQDRFIQFIWTIFVLSMSILLTYATVQLVQEFLNYDLVI-RISIKSNDYSTEXXXXXXXXXXXXGSRAKRYWDENLIMSPTKFNRKIRDQMKHIMENNKGSPYNLFTKDERRIYYQHLSPNDADNLSWDNETFIICSIFRGSTMEVGLGTNC----SMHINKISIPQYFNCFSVTPDDSDPQSRITSITLLIRVSGGMNDTVEHEKSFIPGILNQLSGLRGMIHERFTFPDIENEGFHLEPGKLVEINYAVIKKSKIATPKQPCSKEVR-EKIIDLDKSFVFSQSACFRSSKGWSSLEKCSCFFADTMRMGIPNVTHPYCGNIL-NISFGMLMKRVECLSDWSSEFNKQIKEIFNNKCKSSCVHFQYDLSLSVTKWLPTNWQLGWLIDIDAAGKGFQPDSQQXXXXXXXXXXXXXYINSQDL--FNYPNSGKLSYIDKDNFVMVVIKRRNFDTMEHREKLTLDLYNLISRFXXXXXXXXXXXXAFLLEIVEMIYL 1661
            +KL+    +  L C+ ++IRG+ K+ S+   F++++W++FVL M+I+L  AT  L+ ++  YD+ + +  ++ N   + FP+ + CHHHPF   A + WD   ++SP+ FN  +R    H +  +     N +  D   IYYQ+L  N++  L       + C   R +  ++    +C    S  I ++S PQ+FNC++  P +          +L++ +     D   HE++F+  +  Q  GLR ++HE  T PDIE EG H+EPGKL EIN+  +   K+ TPK+PC  +    K+IDLD  + ++        K +  ++ C C +    R   P    PYCG +   I       R+ C+S++       + E    KC+  C ++ Y+ ++S+TKW    WQL WL     A K    +     + +  K  +++Y+  +DL   N  +S K   +D D+F  VV+K+++    E  EK  L L  L+SR GG CSL +GLT A ++E +E +YL
Sbjct:   34 KKLVKDESILELFCRCMTIRGVNKMTSASS-FLRYLWSVFVLLMTIILVGATCLLILDYFKYDVTLHQRDLQKN---STFPAITICHHHPFSKDAYKLWDSKKVLSPSLFNLYMRQNALHYLNQDDKFANNFYYFDSIDIYYQNLLYNESIALGHKYTIVLYC--LRRNDYQIDFENDCQQFDSYVIKRLSHPQHFNCYTFEPRNISVSVSTRFFSLIVSLGRPEPDYANHEQAFLIDVFEQAKGLRAVVHEPGTIPDIEREGLHVEPGKLNEINFQPVLNLKLDTPKRPCIPDNESSKLIDLDSKYNYTFYEYINLEKQYLIVKHCKCMYILYPRPLYPTKEVPYCGKLWPKIDLDEFTTRINCVSNY---LKMSLTEEIEKKCRPRCSYYTYEATVSITKWRAKQWQLHWLSLQSNAQKALNANI-SAESYQNAKNSWKSYLERRDLSNVNIADSRKSLNLDGDDFAYVVLKKKSSILKERSEKEVLSLPVLLSRIGGFCSLLIGLTVAVVVEFIEFVYL 545
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Planmine SMEST
Match: SMESG000000150.1 (SMESG000000150.1)

HSP 1 Score: 225.713 bits (574), Expect = 2.928e-65
Identity = 167/529 (31.57%), Postives = 283/529 (53.50%), Query Frame = 3 
Query:  108 IYAKCQKLIAMGYLKTLICKTLSIRGIGKIGSSQDRFIQFIWTIFVLSMSILLTYATVQLVQEFLNYDLVIRISIKSNDYSTEXXXXXXXXXXXXGSRAKRYWDENLIMSPTKFNRKIRDQMKHIMENNK---GSPYNLFTKDERRIYYQHLSPNDADNLSWDNETFIICSIFRGSTMEVGLGTNCSMHIN----KISIPQYFNCFSVTPDDSDPQSRITSITLLIRVSGGMNDTVEHEKSFIPGILNQLSGLRGMIHERFTFPDIENEGFHLEPGKLVEINYAVIKKSKIATPKQPCSKEVREKII---DLDKSFVFSQSACFRSSKGWSSLEKCSCFFADTMRMGIPNVTHPYCGNILNISFGMLMKRVECLSDWSSEFNKQIKEIFNNKCKSSCVHFQYDLSLSVTKWLPTNWQLGWLIDIDAAGKGFQPDSQQXXXXXXXXXXXX--XYINSQDLFNYPNSGKLSYIDKDNFVMVVIKRRNFDTMEHREKLTLDLYNLISRFXXXXXXXXXXXXAFLLEIVEMIY 1658
            + ++ +K  ++ ++  L C T SIRG+ K+      F++ +W  FVL M+  LT AT  +V EFL YD+ +   I   D ++ FP+ + CHHHPF  +A   W+ +++MSP K+NR +R       + N+   GS  +    D   +YYQ+L  N++  +  D ET++ C    G    +    +CS   N    ++S+ +YFNC +  P++ +      SI +++ +     D  EHE++FI     +  GLR ++HE  +FPDI  +G H+EPGK+ EI Y  I+  ++ TP +PC +  R+ ++   DLD++F+  +  C    K    ++ C C F    R   P +  PYCG I N +F ++  R+ C+ D  S+ + Q KE+    C   C    ++ ++S+TKW  T W L W+ + + A    + +        E K      +Y N ++L +  ++    ++  +NF  VV++R++ +T+  +EKL    Y L+SR GG+CSL +GLT AF++E +E IY
Sbjct:   53 VQSQRKKKSSISHIFYLFCTTTSIRGMNKVPKG-PIFLRLLWLSFVLIMTTGLTVATTFMVLEFLQYDVNVYTKIIL-DSNSSFPALTICHHHPFSIKAHDLWNRSIVMSPAKYNRYLRKMSNDSFQKNQYGHGSVSSYL--DTMSMYYQNLQMNESFMIGHDKETYLTCMNQLG--FNINFEDDCSKLFNTSFHRLSLHEYFNCHTFQPENINVSYLFESINIVVSLEPKP-DYREHEQAFIVETFQRAEGLRVVVHETGSFPDIAQDGLHVEPGKMNEIKYQPIQHKRVNTPTKPCVE--RDDLMTFKDLDQTFMSDKKNCILLKKQELIMKNCKCIFVSFPRPIEPTLDLPYCGKIQNDNFTVIEDRISCIGDIVSDADYQ-KELERTICFPRCDILTFESTISITKWGATAWHLYWIRETNKALNLLKANVNNSTRVTETKGYKMWLDYANKENLSDVESTKVNHFLQGENFAYVVLQRKSKNTVIKQEKLVTTPYVLLSRIGGVCSLTIGLTAAFVVEFIEFIY 571
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Planmine SMEST
Match: SMESG000039581.1 (SMESG000039581.1)

HSP 1 Score: 225.328 bits (573), Expect = 5.693e-65
Identity = 161/509 (31.63%), Postives = 275/509 (54.03%), Query Frame = 3 
Query:  156 LICKTLSIRGIGKIGSSQDRFIQFIWTIFVLSMSILLTYATVQLVQEFLNYDLVIRISIKSNDYSTEXXXXXXXXXXXXGSRAKRYWDENLIMSPTKFNRKIRD-QMKHIMENNKGSPYNLFTKDERRIYYQHLSPNDADNLSWDNETFIICSIFRGSTMEVGLGTNCS----MHINKISIPQYFNCFSVTPDDSDPQSRITSITLLIRVSGGMNDTVEHEKSFIPGILNQLSGLRGMIHERFTFPDIENEGFHLEPGKLVEINYAVIKKSKIATPKQPCSKEV-REKIIDLDKSFVFSQSACFRSSKGWSSLEKCSCFFADTMRMGIPNVTHPYCGNILNIS-FGMLMKRVECLSDWSSEFNKQIKEIFNNKCKSSCVHFQYDLSLSVTKWLPTNWQLGWLIDIDAAGKGFQPDSQQXXXXXXXXXXXXXYINSQDLFNYPNSGKLSYIDKDNFVMVVIKRRNFDTMEHREKLTLDLYNLISRFXXXXXXXXXXXXAFLLEIVEMIYL 1661
            L C T S++G+ KI +   + ++F+W  FV++MS +L  ++  LV++F+ YD+ I I ++     T FPS + CHHHPF   A +YW EN I+SP+ FNRK+R+    ++M N       +   D   +YYQ++  ND+  +    + F+ C + R +   +    NCS      +   S  ++FNC++          +IT++ +++ + G   D    E++FI     Q  GL+ ++HE    PDI+N G H+EPGKL EI+Y  ++     TP +PC  ++ +E+  DL++ + ++Q  C  S +    +++C C F    R+ IP    PYCGN L  S    L  R+ECL++   + N + K++ + KC   C +  Y+ S+S+TKW  T W L W+       +  + + Q       + K++  Y N+ +L       K  ++  DNF  +V++R + +++   E+L +  Y L+SR GGLCS  +G+T +F +EI+E +YL
Sbjct:   81 LFCSTTSMKGMNKI-TRGPKPLRFLWLSFVMTMSTVLIVSSCLLVRDFMLYDVNINIEVRLAS-QTPFPSITICHHHPFSMEAYKYWRENKILSPSTFNRKMRNLTHSNLMGNRVMEAEGISNYDVISVYYQNIGYNDSMLIGHQKDIFLSCMLLRNNM--ISYEENCSKLEEYEVKMFSHHEHFNCYTFEAK----VDKITTLGIIVSM-GPPPDYSATEQAFIVDTFRQAQGLKVIVHEPGVPPDIDNGGVHVEPGKLNEISYRPVEWKLENTPTKPCFNDIKKERYNDLERFYNYTQDMCLSSEEQKRIIDECHCIFIYNPRLQIPTKKLPYCGNFLEDSDLSELNHRIECLTNIQEDVNFK-KKLESEKCFQRCTYMTYETSISITKWRATEWHLHWIRLQLLTFRQLKNNPQINATESTYFKQWLKYWNATNLTVIDEDRKERFLANDNFAYIVLRRESDNSVRKYERLVISPYVLVSRIGGLCSFTVGITASFFVEILEFLYL 579
[Close]
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Planmine SMEST
Match: SMESG000043119.1 (SMESG000043119.1)

HSP 1 Score: 211.075 bits (536), Expect = 8.558e-60
Identity = 159/513 (30.99%), Postives = 266/513 (51.85%), Query Frame = 3 
Query:  156 LICKTLSIRGIGKIGSSQDRFIQFIWTIFVLSMSILLTYATVQLVQEFLNYDLVIRISIKSNDYSTEXXXXXXXXXXXXGSRAKRYWDENLIMSPTKFNRKIRD-QMKHIMENNKGSPYNLFTKDERRIYYQHLSPNDADNLSWDNETFIICSIFRGSTMEVGLGTNCS----MHINKISIPQYFNCFSVTPDDSDPQSRITSITLLIRVSGGMNDTVEHEKSFIPGILNQLSGLRGMIHERFTFPDIENEGFHLEPGKLVEINYAVIKKSKIATPKQPCSKEV-REKIIDLDKSFVFSQSACFRSSKGWSSLEKCSCFFADTMRMGIPNVTHPYCGNIL-NISFGMLMKRVECLSDWSSEFNKQIKEIFNNKCKSSCVHFQYDLSLSVTKWLPTNWQLGWL----IDIDAAGKGFQPDSQQXXXXXXXXXXXXXYINSQDLFNYPNSGKLSYIDKDNFVMVVIKRRNFDTMEHREKLTLDLYNLISRFXXXXXXXXXXXXAFLLEIVEMIYL 1661
            L C T+SIRGI KI     R ++ +W  FVL MS +L  ++  LV++F+ YD+ I   ++  D  T FP+ + CHHHPF  R  R W     MSP+ FNRK+R+  +  ++ N+  S   +   D   IYYQ+L    A  +  ++  F+ C     +  ++    NC+      I  +S  ++FNC++  P      S +  + +++ + G      + E+SFI     Q  GL+ ++HE  T PDI+  G H+EPGK+ EI Y   +     TP +PC  +  R   +DL  ++ +++  C + ++    + +C C F    R  +PN + PYCG+IL + +   +   V+CLSD  ++ +K+ ++    KC   C +  +D ++S+TKW  T W L WL      +    +  + +  +     +  K +  Y N +DL           +  DNF  +V++R + D+++  E+L +  Y L+SR GGLCSL +G+T AF++E+VE  YL
Sbjct:   63 LFCSTISIRGINKIIKG-PRPLRLLWLCFVLIMSAILITSSCLLVRDFMLYDVNINTKLEI-DSMTPFPALTVCHHHPFSHRGYRMWKSGKAMSPSYFNRKMRNLTLDQLLLNDVESAETISYYDGISIYYQNLPYEQAIQMGHNSSIFLTCMYLLEN--QIYFAENCTKLEFYRIKLLSHHEHFNCYTFEPTQPHVSSNLDMLGIIVSL-GPEPKHFDMEQSFIIDTFRQAQGLKVIVHESGTLPDIDEAGLHVEPGKMNEIIYRPNQWQLTNTPAKPCINDTSRIAYVDLANTYNYTKHLCLKYAEQEDIMNQCHCMFIYNPRPMMPNESMPYCGSILKDFNLSNIYSYVKCLSDVLNQKSKR-QQWEKEKCFHRCNYMTFDSTISITKWRATEWHLYWLRQQITSLGEMKQFLKANPNRNVTESQAFKTWLIYGNKKDLRKTGLKANEFLLKDDNFAYIVLRRESKDSVKKYERLVITQYVLVSRIGGLCSLTVGITLAFIVELVEFCYL 569
The following BLAST results are available for this feature:
BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
SCNN1B1.178e-624.60sodium channel epithelial 1 beta subunit [Source:H... [more]
SCNN1B1.237e-624.60sodium channel epithelial 1 beta subunit [Source:H... [more]
SCNN1B1.346e-624.60sodium channel epithelial 1 beta subunit [Source:H... [more]
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Scnn1b8.787e-622.89sodium channel, nonvoltage-gated 1 beta [Source:MG... [more]
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q92075|SCNNA_CHICK2.302e-622.22Amiloride-sensitive sodium channel subunit alpha O... [more]
sp|P51168|SCNNB_HUMAN5.657e-624.60Amiloride-sensitive sodium channel subunit beta OS... [more]
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4S2JQK41.381e-6832.60Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A267EKX12.328e-6630.59Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8HS761.191e-6530.78Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8G5D01.225e-6530.51G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A267F1601.377e-6530.78Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of FMRFamide-activated amiloride-sensitive sodium channel vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000034894.10.000e+0100.00SMESG000034894.1[more]
SMESG000060702.17.881e-6631.03SMESG000060702.1[more]
SMESG000000150.12.928e-6531.57SMESG000000150.1[more]
SMESG000039581.15.693e-6531.63SMESG000039581.1[more]
SMESG000043119.18.558e-6030.99SMESG000043119.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027466 ID=SMED30027466|Name=FMRFamide-activated amiloride-sensitive sodium channel|organism=Schmidtea mediterranea sexual|type=transcript|length=1795bp
GTAATGGTTTATGGAAATTGCACCCATCCGTCCTATCGATTTTTGAGTGT
AGAATGTGTGTAAGTGTGTAAATGTGTTAAAATTATTATTTTGAAAAATT
CTGTAAAATATATGCCAAGTGTCAGAAATTGATAGCAATGGGATATTTGA
AAACCTTGATCTGCAAGACCTTGTCTATCCGAGGAATTGGCAAAATCGGG
AGCTCTCAAGACAGATTCATTCAGTTTATTTGGACAATTTTCGTACTATC
AATGTCAATTCTTTTGACCTACGCAACTGTGCAATTAGTTCAGGAATTTC
TCAACTATGACTTGGTGATACGAATCTCAATTAAATCTAACGACTATTCC
ACGGAATTTCCCTCTTTTTCATTCTGTCATCATCACCCATTTGGATCTCG
GGCTAAAAGATACTGGGATGAAAATTTGATAATGTCGCCGACAAAGTTTA
ACCGAAAAATCCGAGATCAAATGAAACACATAATGGAGAATAACAAAGGG
TCTCCTTACAATCTATTTACAAAAGATGAAAGACGGATTTATTATCAACA
TTTATCCCCGAATGATGCCGATAATCTCAGTTGGGACAACGAAACATTTA
TTATTTGCAGCATTTTCAGAGGCTCCACCATGGAAGTCGGCCTCGGTACC
AATTGTTCGATGCATATAAACAAAATTAGTATTCCTCAATATTTCAACTG
TTTCAGTGTTACTCCGGACGATTCCGATCCACAATCAAGAATCACATCTA
TTACTCTGTTAATTCGCGTTTCCGGCGGAATGAATGATACAGTTGAACAT
GAGAAAAGTTTCATTCCGGGTATTCTCAATCAGCTAAGCGGATTGCGAGG
AATGATTCATGAAAGGTTCACCTTTCCTGACATTGAAAATGAAGGATTTC
ACTTGGAACCTGGTAAGCTCGTAGAAATCAATTATGCAGTTATTAAAAAA
AGTAAAATTGCAACACCAAAACAACCGTGTTCAAAAGAGGTTCGGGAAAA
GATAATAGATCTTGATAAGTCATTTGTATTTTCACAGTCAGCTTGCTTTA
GATCATCGAAAGGATGGAGCAGTTTGGAGAAATGTTCATGTTTTTTCGCC
GATACCATGAGAATGGGTATTCCAAACGTCACCCATCCGTATTGTGGAAA
TATCCTAAATATCTCTTTTGGAATGCTCATGAAAAGAGTTGAATGCCTAA
GTGATTGGAGTTCAGAGTTTAATAAGCAAATAAAAGAAATCTTTAATAAC
AAATGCAAATCGAGCTGTGTACATTTCCAGTACGATTTAAGTCTCTCTGT
GACAAAATGGTTACCAACGAACTGGCAGCTTGGTTGGTTAATAGACATAG
ATGCAGCAGGTAAGGGTTTCCAACCCGATTCTCAACAGGAAAATGCAAAC
GAAGAATTCAAAAAGAAATTCGAAAATTACATCAATTCACAAGATCTTTT
CAACTATCCAAATAGTGGCAAGCTGTCTTATATCGACAAAGACAACTTTG
TCATGGTTGTCATTAAACGCCGAAATTTTGACACGATGGAACATAGAGAA
AAGTTAACTTTGGATTTGTACAATCTAATCAGTCGGTTCGGTGGATTGTG
CTCGCTATGTCTAGGGCTAACGTGTGCATTTCTTTTGGAGATTGTCGAGA
TGATCTATTTAGCGGCTGAAGACCATAACAAAAAGAAAAAAGAAAAAAGA
AAAATAATCCATAGTGAAGAATTGGAAATTGATGGGAAAGTATTAGACAA
AACGAGATCAGAATCCCCGATTTGGCTTAGTTCTTTGTAACTCAA
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protein sequence of SMED30027466-orf-1

>SMED30027466-orf-1 ID=SMED30027466-orf-1|Name=SMED30027466-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=551bp
MGYLKTLICKTLSIRGIGKIGSSQDRFIQFIWTIFVLSMSILLTYATVQL
VQEFLNYDLVIRISIKSNDYSTEFPSFSFCHHHPFGSRAKRYWDENLIMS
PTKFNRKIRDQMKHIMENNKGSPYNLFTKDERRIYYQHLSPNDADNLSWD
NETFIICSIFRGSTMEVGLGTNCSMHINKISIPQYFNCFSVTPDDSDPQS
RITSITLLIRVSGGMNDTVEHEKSFIPGILNQLSGLRGMIHERFTFPDIE
NEGFHLEPGKLVEINYAVIKKSKIATPKQPCSKEVREKIIDLDKSFVFSQ
SACFRSSKGWSSLEKCSCFFADTMRMGIPNVTHPYCGNILNISFGMLMKR
VECLSDWSSEFNKQIKEIFNNKCKSSCVHFQYDLSLSVTKWLPTNWQLGW
LIDIDAAGKGFQPDSQQENANEEFKKKFENYINSQDLFNYPNSGKLSYID
KDNFVMVVIKRRNFDTMEHREKLTLDLYNLISRFGGLCSLCLGLTCAFLL
EIVEMIYLAAEDHNKKKKEKRKIIHSEELEIDGKVLDKTRSESPIWLSSL
*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR001873ENaC
Vocabulary: biological process
TermDefinition
GO:0006814sodium ion transport
GO:0006810transport
GO:0006811ion transport
GO:0035725sodium ion transmembrane transport
Vocabulary: molecular function
TermDefinition
GO:0005272sodium channel activity
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001873Epithelial sodium channelPRINTSPR01078AMINACHANNELcoord: 70..86
score: 25.41
coord: 373..393
score: 28.76
coord: 176..192
score: 23.06
coord: 235..253
score: 31.79
IPR001873Epithelial sodium channelPFAMPF00858ASCcoord: 9..504
e-value: 1.7E-49
score: 169.0
IPR001873Epithelial sodium channelPANTHERPTHR11690AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATEDcoord: 12..522
NoneNo IPR availableTMHMMTMhelixcoord: 27..49