SMED30027376
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30027376 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 29
Homology
BLAST of SMED30027376 vs. Planmine SMEST
Match: SMESG000007017.1 (SMESG000007017.1) HSP 1 Score: 1753.8 bits (4541), Expect = 0.000e+0 Identity = 1042/1042 (100.00%), Postives = 1042/1042 (100.00%), Query Frame = 2 Query: 17 AERLSKLCKEFSESILPDSTVIRNXXXXXXXXXXXSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNXXXXXXXXXXXXXXXXXXXXGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCXXXXXXXXXXXXXXXXXXCKEFSESILPDSTVIRNXXXXXXXXXXXSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNXXXXXXXXXXXXXXXXXXXXGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCKSKELDLKNVDPILDVSDCHSRPFSDFEDFIVPAVHVPVHSFGRPYLSYSDSINEDLSHNLDYNQPIVDDRELVCSISSQCSQIEMITRSDDLADDVSLSFQSQLDPSIPEFEKSSEIGYYAXXXXXXXXXXXXXXXXXXXNYQFSEEERNLNLDSLKPSNWMHIFEMELKYLEGQTDAFSSATDKNQEHFDIGCDHFFDNDKLFRVTENASLRLKNLENNCSNYVSNRXXXXXXXXXXXXXXXXXXXXXXXXXXHLFHLLDLLKRQNEXXXXXXXXXRDDIEKQLQEHRNRISDVRSRLSRYYKCISSISTEKVNLDKKNSEDKQPPSIWRKYKEKHIIPLQSFRSQRTNLSPSNSTQSLYRKSNFDIQKSKKLLEETENIIRNSREKLNKK 3142 AERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNEISESKLIELEEILLKEVSEGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNEISESKLIELEEILLKEVSEGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCKSKELDLKNVDPILDVSDCHSRPFSDFEDFIVPAVHVPVHSFGRPYLSYSDSINEDLSHNLDYNQPIVDDRELVCSISSQCSQIEMITRSDDLADDVSLSFQSQLDPSIPEFEKSSEIGYYADSDVFSHFSSSDLPLFPPSNYQFSEEERNLNLDSLKPSNWMHIFEMELKYLEGQTDAFSSATDKNQEHFDIGCDHFFDNDKLFRVTENASLRLKNLENNCSNYVSNRRSSNASIPINAASRISSPFPDSDSPSHLFHLLDLLKRQNEIILSSSSLSRDDIEKQLQEHRNRISDVRSRLSRYYKCISSISTEKVNLDKKNSEDKQPPSIWRKYKEKHIIPLQSFRSQRTNLSPSNSTQSLYRKSNFDIQKSKKLLEETENIIRNSREKLNKK Sbjct: 941 AERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNEISESKLIELEEILLKEVSEGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNEISESKLIELEEILLKEVSEGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCKSKELDLKNVDPILDVSDCHSRPFSDFEDFIVPAVHVPVHSFGRPYLSYSDSINEDLSHNLDYNQPIVDDRELVCSISSQCSQIEMITRSDDLADDVSLSFQSQLDPSIPEFEKSSEIGYYADSDVFSHFSSSDLPLFPPSNYQFSEEERNLNLDSLKPSNWMHIFEMELKYLEGQTDAFSSATDKNQEHFDIGCDHFFDNDKLFRVTENASLRLKNLENNCSNYVSNRRSSNASIPINAASRISSPFPDSDSPSHLFHLLDLLKRQNEIILSSSSLSRDDIEKQLQEHRNRISDVRSRLSRYYKCISSISTEKVNLDKKNSEDKQPPSIWRKYKEKHIIPLQSFRSQRTNLSPSNSTQSLYRKSNFDIQKSKKLLEETENIIRNSREKLNKK 1982 HSP 2 Score: 1063.91 bits (2750), Expect = 0.000e+0 Identity = 649/649 (100.00%), Postives = 649/649 (100.00%), Query Frame = 2 Query: 17 AERLSKLCKEFSESILPDSTVIRNXXXXXXXXXXXSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNXXXXXXXXXXXXXXXXXXXXGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCXXXXXXXXXXXXXXXXXXCKEFSESILPDSTVIRNXXXXXXXXXXXSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNXXXXXXXXXXXXXXXXXXXXGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDC 1963 AERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNEISESKLIELEEILLKEVSEGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNEISESKLIELEEILLKEVSEGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDC Sbjct: 281 AERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNEISESKLIELEEILLKEVSEGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNEISESKLIELEEILLKEVSEGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDC 929 HSP 3 Score: 978.778 bits (2529), Expect = 0.000e+0 Identity = 592/593 (99.83%), Postives = 592/593 (99.83%), Query Frame = 2 Query: 185 GELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNXXXXXXXXXXXXXXXXXXXXGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCXXXXXXXXXXXXXXXXXXCKEFSESILPDSTVIRNXXXXXXXXXXXSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNXXXXXXXXXXXXXXXXXXXXGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDC 1963 ELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNEISESKLIELEEILLKEVSEGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNEISESKLIELEEILLKEVSEGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDC Sbjct: 7 SELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNEISESKLIELEEILLKEVSEGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNEISESKLIELEEILLKEVSEGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDC 599
BLAST of SMED30027376 vs. Planmine SMEST
Match: SMESG000007018.1 (SMESG000007018.1) HSP 1 Score: 408.297 bits (1048), Expect = 3.867e-129 Identity = 316/474 (66.67%), Postives = 350/474 (73.84%), Query Frame = 2 Query: 617 KDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCXXXXXXXXXXXXXXXXXXCKEFSESILPDSTVIRNXXXXXXXXXXXSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNXXXXXXXXXXXXXXXXXXXXGK------DLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVE--NSKNDQIKLKGQDLSKLSKV-----------------MDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDC 1963 KDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIE L+ + NSKNDQIKLKGQDLSKLSKVMDKIE +VIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE +D++I E KSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNEISESKLIELEEILLKEVSE K D +KLK L ++ +I+S I + D R +E + ++ +E+++ +L E + K +I ++ + K+SK+ +D ++ ++E P + K NKTLLESVVPKLQEVIDC Sbjct: 160 KDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIENNLERISRNSKNDQIKLKGQDLSKLSKVMDKIE------------------------------KVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE------VDRNIWE-------KSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNEISESKLIELEEILLKEVSEEKISNSKNDQIKLKGQDLSKLSKVM---DKIESKINETPD---AERSNEWKANKTLLESVVPKLQEVIDCKIQRIGIEERRSRKMSKLCKEFSESILPDSTVIRNVDIVDEIVDEIPTS-----GKQNKTLLESVVPKLQEVIDC 579 HSP 2 Score: 363.999 bits (933), Expect = 1.979e-112 Identity = 305/445 (68.54%), Postives = 337/445 (75.73%), Query Frame = 2 Query: 17 AERLSKLCKEFSESILPDSTVIRNXXXXXXXXXXXSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNXXXXXXXXXXXXXXXXXXXXGK------DLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVE--NSKNDQIKLKGQDLSKLSKV-----------------MDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCXXXXXXXXXXXXXXXXXXCKEFSESILPDSTVIRNXXXXXXXXXXXSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRF 1276 AERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE +D++I E KSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNEISESKLIELEEILLKEVSE K D +KLK L ++ +I+S I + D R +E + ++ +E+++ +L E + K +I ++ + K+SK+ +D ++ ++E P + K NKTLLESVVPKLQEVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKER ELENSKGKELVESLEIMKSLIPKSKAGEKER+ Sbjct: 260 AERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE------VDRNIWE-------KSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNEISESKLIELEEILLKEVSEEKISNSKNDQIKLKGQDLSKLSKVM---DKIESKINETPD---AERSNEWKANKTLLESVVPKLQEVIDCKIQRIGIEERRSRKMSKLCKEFSESILPDSTVIRNVDIVDEIVDEIPTS-----GKQNKTLLESVVPKLQEVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKER------------------ELENSKGKELVESLEIMKSLIPKSKAGEKERKI 662 HSP 3 Score: 233.417 bits (594), Expect = 4.670e-65 Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 2 Query: 854 MDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCXXXXXXXXXXXXXXXXXXCKEFSESILPDSTVIRNXXXXXXXXXXXSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE 1315 MDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE Sbjct: 1 MDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE 154 HSP 4 Score: 173.326 bits (438), Expect = 1.019e-44 Identity = 103/103 (100.00%), Postives = 103/103 (100.00%), Query Frame = 2 Query: 17 AERLSKLCKEFSESILPDSTVIRNXXXXXXXXXXXSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE 325 AERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE Sbjct: 52 AERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE 154 HSP 5 Score: 84.3445 bits (207), Expect = 2.561e-16 Identity = 40/40 (100.00%), Postives = 40/40 (100.00%), Query Frame = 2 Query: 1844 MDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDC 1963 MDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDC Sbjct: 1 MDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDC 40
BLAST of SMED30027376 vs. Planmine SMEST
Match: SMESG000007018.1 (SMESG000007018.1) HSP 1 Score: 394.045 bits (1011), Expect = 1.486e-126 Identity = 255/310 (82.26%), Postives = 261/310 (84.19%), Query Frame = 2 Query: 614 GKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCXXXXXXXXXXXXXXXXXXCKEFSESILPDSTVIRNXXXXXXXXXXXSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRN 1543 GKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIE L+ + NSKNDQIKLKGQDLSKLSKVMDKIE +VIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE +D++I E KSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRN Sbjct: 159 GKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIENNLERISRNSKNDQIKLKGQDLSKLSKVMDKIE------------------------------KVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE------VDRNIWE-------KSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRN 425 HSP 2 Score: 272.322 bits (695), Expect = 4.042e-81 Identity = 162/179 (90.50%), Postives = 165/179 (92.18%), Query Frame = 2 Query: 17 AERLSKLCKEFSESILPDSTVIRNXXXXXXXXXXXSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLESGSKVKIDKSICEQLKAFGKKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRN 553 AERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE +D++I E KSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRN Sbjct: 260 AERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE------VDRNIWE-------KSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRN 425 HSP 3 Score: 233.802 bits (595), Expect = 3.547e-67 Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 2 Query: 854 MDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCXXXXXXXXXXXXXXXXXXCKEFSESILPDSTVIRNXXXXXXXXXXXSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE 1315 MDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE Sbjct: 1 MDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDCKSKELELKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE 154 HSP 4 Score: 172.94 bits (437), Expect = 3.785e-46 Identity = 103/103 (100.00%), Postives = 103/103 (100.00%), Query Frame = 2 Query: 17 AERLSKLCKEFSESILPDSTVIRNXXXXXXXXXXXSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE 325 AERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE Sbjct: 52 AERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENGTKLICDALEKGELENSKGKELVESLEIMKSLIPKSKAGEKERRFVEEIIDDLNRSLE 154 HSP 5 Score: 153.295 bits (386), Expect = 2.236e-39 Identity = 79/89 (88.76%), Postives = 82/89 (92.13%), Query Frame = 2 Query: 1604 GKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQIKLKGQDLSKLSKVMDKIESKIN 1870 GKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIE L+ + NSKNDQIKLKGQDLSKLSKVMDKIE I+ Sbjct: 159 GKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIENNLERISRNSKNDQIKLKGQDLSKLSKVMDKIEKVID 247 HSP 6 Score: 84.7297 bits (208), Expect = 9.911e-17 Identity = 40/40 (100.00%), Postives = 40/40 (100.00%), Query Frame = 2 Query: 1844 MDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDC 1963 MDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDC Sbjct: 1 MDKIESKINETPDAERSNEWKANKTLLESVVPKLQEVIDC 40
BLAST of SMED30027376 vs. Planmine SMEST
Match: SMESG000020529.1 (SMESG000020529.1) HSP 1 Score: 160.229 bits (404), Expect = 4.313e-45 Identity = 108/143 (75.52%), Postives = 117/143 (81.82%), Query Frame = 2 Query: 383 KKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNXXXXXXXXXXXXXXXXXXXXGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQ 811 KKSE +L+NSDEDS KILEAIKCL DDEE +QEKLLNR D DNL DGIKV + +SE KLI+LEE LLKEVSEGKDLVKLK TV E LIGILCEAVE Q SIPDIADRIRKS QD ENS R IETILKEL+ENS+ND+ Sbjct: 5 KKSEIQLNNSDEDSKKILEAIKCLIDDEEIQQEKLLNRTDTDNLFDGIKVTE------VSELKLIQLEENLLKEVSEGKDLVKLKPTVIETLIGILCEAVEFQFSIPDIADRIRKSEQDGNENSLRSIETILKELIENSRNDK 141 HSP 2 Score: 160.229 bits (404), Expect = 4.313e-45 Identity = 108/143 (75.52%), Postives = 117/143 (81.82%), Query Frame = 2 Query: 1373 KKSEKRLDNSDEDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNXXXXXXXXXXXXXXXXXXXXGKDLVKLKATVHEPLIGILCEAVEIQSSIPDIADRIRKSRQDEIENSRRPIETILKELVENSKNDQ 1801 KKSE +L+NSDEDS KILEAIKCL DDEE +QEKLLNR D DNL DGIKV + +SE KLI+LEE LLKEVSEGKDLVKLK TV E LIGILCEAVE Q SIPDIADRIRKS QD ENS R IETILKEL+ENS+ND+ Sbjct: 5 KKSEIQLNNSDEDSKKILEAIKCLIDDEEIQQEKLLNRTDTDNLFDGIKVTE------VSELKLIQLEENLLKEVSEGKDLVKLKPTVIETLIGILCEAVEFQFSIPDIADRIRKSEQDGNENSLRSIETILKELIENSRNDK 141
BLAST of SMED30027376 vs. Planmine SMEST
Match: SMESG000028712.1 (SMESG000028712.1) HSP 1 Score: 108.227 bits (269), Expect = 3.236e-26 Identity = 76/93 (81.72%), Postives = 83/93 (89.25%), Query Frame = 2 Query: 416 EDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNXXXXXXXXXXXXXXXXXXXXGKDLVKLKATVHEPLIGILCEAVEIQS 694 ED+NKILEAIKCLFDD+E EQEKLLNRKDIDNL+DGIKV KD L NEISESK+IELEE LLK+VSEGKD VKLK T +E LI ILCE+VEIQS Sbjct: 131 EDNNKILEAIKCLFDDQESEQEKLLNRKDIDNLIDGIKVTKDTLSNEISESKVIELEENLLKDVSEGKDFVKLKETDNESLIAILCESVEIQS 223 HSP 2 Score: 108.227 bits (269), Expect = 3.236e-26 Identity = 76/93 (81.72%), Postives = 83/93 (89.25%), Query Frame = 2 Query: 1406 EDSNKILEAIKCLFDDEECEQEKLLNRKDIDNLLDGIKVAKDALRNXXXXXXXXXXXXXXXXXXXXGKDLVKLKATVHEPLIGILCEAVEIQS 1684 ED+NKILEAIKCLFDD+E EQEKLLNRKDIDNL+DGIKV KD L NEISESK+IELEE LLK+VSEGKD VKLK T +E LI ILCE+VEIQS Sbjct: 131 EDNNKILEAIKCLFDDQESEQEKLLNRKDIDNLIDGIKVTKDTLSNEISESKVIELEENLLKDVSEGKDFVKLKETDNESLIAILCESVEIQS 223 The following BLAST results are available for this feature:
BLAST of SMED30027376 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30027376 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30027376 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30027376 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30027376 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30027376 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30027376 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30027376 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30027376 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30027376 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30027376 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30027376 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30027376 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30027376 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30027376 ID=SMED30027376|Name=SMED30027376|organism=Schmidtea mediterranea sexual|type=transcript|length=3220bpback to top protein sequence of SMED30027376-orf-1 >SMED30027376-orf-1 ID=SMED30027376-orf-1|Name=SMED30027376-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1048bp LKKEEAERLSKLCKEFSESILPDSTVIRNVDIVDEIVDEVSKERKVGLENback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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