DnaJ-like protein

Overview
NameDnaJ-like protein
Smed IDSMED30027362
Length (bp)1383
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DnaJ-like protein (SMED30027362) t-SNE clustered cells

Violin plots show distribution of expression levels for DnaJ-like protein (SMED30027362) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DnaJ-like protein (SMED30027362) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DnaJ-like protein (SMED30027362) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30027362

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
posterior sideSMED30027362h1SMcG0016774 dd_Smed_v4_3460_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30027362h1SMcG0016774 dd_Smed_v4_3460_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30027362h1SMcG0016774 dd_Smed_v6_3460_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of DnaJ-like protein vs. Ensembl Human
Match: DNAJB11 (DnaJ heat shock protein family (Hsp40) member B11 [Source:HGNC Symbol;Acc:HGNC:14889])

HSP 1 Score: 414.461 bits (1064), Expect = 7.099e-143
Identity = 217/350 (62.00%), Postives = 262/350 (74.86%), Query Frame = 1
Query:  106 LLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKD--IPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGLGGY 1149
            LLL + +  +  GRDFYKIL VPRSA+   IKKAYR LA QLHPD+N DDP A+ +FQDL AAYE LSD EKRK +D  GEEGLKD H   S  D FS FFGD  F  GG+ RQ+D  IPRG D+++DL V+LEE+Y GNFVEVVR K V + A G R+CNCR EM+T Q GPGRF MTQE VCDECPNV+ V EE+ LEVEIEPG+RDG  YPF+ EGEPH+DGEPGDL+FRI+   HP FERRGDDLYTNVTISL+++L GF M+I HLDGHKV + R+K+T PGA + K  EG+PN++NN++KGSL IT DV FPK+++  E  + I + L    +  + YNGL GY
Sbjct:   11 LLLLYLIGAVIAGRDFYKILGVPRSASIKDIKKAYRKLALQLHPDRNPDDPQAQEKFQDLGAAYEVLSDSEKRKQYDTYGEEGLKDGHQS-SHGDIFSHFFGDFGFMFGGTPRQQDRNIPRGSDIIVDLEVTLEEVYAGNFVEVVRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHPIFERRGDDLYTNVTISLVESLVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAREGIKQLLKQGSVQKV-YNGLQGY 358          
BLAST of DnaJ-like protein vs. Ensembl Human
Match: DNAJB11 (DnaJ heat shock protein family (Hsp40) member B11 [Source:HGNC Symbol;Acc:HGNC:14889])

HSP 1 Score: 414.461 bits (1064), Expect = 7.099e-143
Identity = 217/350 (62.00%), Postives = 262/350 (74.86%), Query Frame = 1
Query:  106 LLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKD--IPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGLGGY 1149
            LLL + +  +  GRDFYKIL VPRSA+   IKKAYR LA QLHPD+N DDP A+ +FQDL AAYE LSD EKRK +D  GEEGLKD H   S  D FS FFGD  F  GG+ RQ+D  IPRG D+++DL V+LEE+Y GNFVEVVR K V + A G R+CNCR EM+T Q GPGRF MTQE VCDECPNV+ V EE+ LEVEIEPG+RDG  YPF+ EGEPH+DGEPGDL+FRI+   HP FERRGDDLYTNVTISL+++L GF M+I HLDGHKV + R+K+T PGA + K  EG+PN++NN++KGSL IT DV FPK+++  E  + I + L    +  + YNGL GY
Sbjct:   11 LLLLYLIGAVIAGRDFYKILGVPRSASIKDIKKAYRKLALQLHPDRNPDDPQAQEKFQDLGAAYEVLSDSEKRKQYDTYGEEGLKDGHQS-SHGDIFSHFFGDFGFMFGGTPRQQDRNIPRGSDIIVDLEVTLEEVYAGNFVEVVRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHPIFERRGDDLYTNVTISLVESLVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAREGIKQLLKQGSVQKV-YNGLQGY 358          
BLAST of DnaJ-like protein vs. Ensembl Human
Match: AC068631.3 (novel protein)

HSP 1 Score: 188.348 bits (477), Expect = 2.112e-57
Identity = 86/131 (65.65%), Postives = 108/131 (82.44%), Query Frame = 1
Query:  673 FVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKV 1065
             V EE+ LEVEIEPG+RDG  YPF+ EGEPH+DGEPGDL+FRI+   HP FERRGDDLYTNVTISL+++L GF M+I HLDGHKV + R+K+T PGA + K  EG+PN++NN++KGSL IT DV FPK+++
Sbjct:    2 LVNEERTLEVEIEPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHPIFERRGDDLYTNVTISLVESLVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQL 132          
BLAST of DnaJ-like protein vs. Ensembl Human
Match: DNAJA2 (DnaJ heat shock protein family (Hsp40) member A2 [Source:HGNC Symbol;Acc:HGNC:14884])

HSP 1 Score: 175.252 bits (443), Expect = 2.355e-49
Identity = 121/335 (36.12%), Postives = 168/335 (50.15%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGG---DVVMDLFVSLEELYNGNFVEVVRYKSVKKSA--------HGTRRCN-CRHEMKTI---QTGPGRFTMTQEQV---------------CDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHK--VKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKD 1059
             Y IL VP  A+ N++KKAYR LAK+ HPDKN   PNA  +F++++ AYE LS+PEKR+++D  GE+GL++  GGG  MD   S             + +         D++  L VSLE+LYNG   ++   K+V  SA           ++C+ CR     I   Q  PG     Q                  C +C   + + E K+LEV ++ GM+ GQ   F  E +     EPGD+   +++  H  F+R G+DL+    I L++AL GF    KHLDG +  VK    KV  PG       EGMP Y N   KG LYI  DV FP++
Sbjct:    9 LYDILGVPPGASENELKKAYRKLAKEYHPDKN---PNAGDKFKEISFAYEVLSNPEKRELYDRYGEQGLREGSGGGGGMDDIFSHIFGGGLFGFMGNQSRSRNGRRRGEDMMHPLKVSLEDLYNGKTTKLQLSKNVLCSACSGQGGKSGAVQKCSACRGRGVRIMIRQLAPGMVQQMQSVCSDCNGEGEVINEKDRCKKCEGKKVIKEVKILEVHVDKGMKHGQRITFTGEADQAPGVEPGDIVLLLQEKEHEVFQRDGNDLHMTYKIGLVEALCGFQFTFKHLDGRQIVVKYPPGKVIEPGCVRVVRGEGMPQYRNPFEKGDLYIKFDVQFPEN 340          
BLAST of DnaJ-like protein vs. Ensembl Human
Match: DNAJA1 (DnaJ heat shock protein family (Hsp40) member A1 [Source:HGNC Symbol;Acc:HGNC:5229])

HSP 1 Score: 172.555 bits (436), Expect = 1.387e-48
Identity = 122/343 (35.57%), Postives = 171/343 (49.85%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGG---SSMDPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSV-------KKSAHGTRRC--NCR---HEMKTIQTGPGRFTMTQEQV---------------CDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKS------MEGMPNYENNHVKGSLYITIDVAFPKDKVYS 1071
            +Y +L V  +A   ++KKAYR LA + HPDKN   PN   +F+ ++ AYE LSD +KR+++D  GE+ +K+   GG   S MD F  FFG      GG R Q++  RG +VV  L V+LE+LYNG   ++   K+V       +    G   C  NCR    +++  Q GPG     Q                  C  C   + V E+K+LEV I+ GM+DGQ   F  EG+     EPGD+   + Q  H  F RRG+DL+  + I L++AL GF   I  LD   + +     + PG  +K         EGMP Y   + KG L I   V FP++   S
Sbjct:    7 YYDVLGVKPNATQEELKKAYRKLALKYHPDKN---PNEGEKFKQISQAYEVLSDAKKRELYDKGGEQAIKEGGAGGGFGSPMDIFDMFFG------GGGRMQRE-RRGKNVVHQLSVTLEDLYNGATRKLALQKNVICDKCEGRGGKKGAVECCPNCRGTGMQIRIHQIGPGMVQQIQSVCMECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKDGQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVI----TSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLS 335          
BLAST of DnaJ-like protein vs. Ensembl Celegans
Match: dnj-20 (DnaJ homolog dnj-20 [Source:UniProtKB/Swiss-Prot;Acc:Q8MPX3])

HSP 1 Score: 390.578 bits (1002), Expect = 6.482e-134
Identity = 208/353 (58.92%), Postives = 262/353 (74.22%), Query Frame = 1
Query:   85 LMNFLIILLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGL-KDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGL 1140
            ++N  +++L S  V  + CGRDFYKIL V ++AN+NQIKKAYR LAK+LHPD+N+DD  A  +FQDL++AYE LSD EKR ++D  GEEG+ K   GGG   DPFSSFFGD F   GG   ++  P+G DV +DLFV+LEE+YNG+FVE+ R K+V K   GTR+CNCRHEM+T Q G GRF M Q +VCDECPNV+ V E K+LEVE+E G  +G    F  EGEPHI+G+PGDLKF+IR   HPRFER+GDDLYTNVTISL DAL GF MEI+HLDGH VKV R+KVTWPGA ++K  EGMP+ E+N+ KG L +T DV FPK ++  E++ +I++ L    + P  YNGL
Sbjct:    3 ILNVSLLVLASSLVAFVECGRDFYKILGVAKNANANQIKKAYRKLAKELHPDRNQDDEMANEKFQDLSSAYEVLSDKEKRAMYDRHGEEGVAKMGGGGGGGHDPFSSFFGDFFGGGGGHGGEEGTPKGADVTIDLFVTLEEVYNGHFVEIKRKKAVYKQTSGTRQCNCRHEMRTEQMGQGRFQMFQVKVCDECPNVKLVQENKVLEVEVEVGADNGHQQIFHGEGEPHIEGDPGDLKFKIRIQKHPRFERKGDDLYTNVTISLQDALNGFEMEIQHLDGHIVKVQRDKVTWPGARLRKKDEGMPSLEDNNKKGMLVVTFDVEFPKTELSDEQKAQIIEILQQNTVKPKAYNGL 355          
BLAST of DnaJ-like protein vs. Ensembl Celegans
Match: dnj-13 (DNaJ domain (Prokaryotic heat shock protein) [Source:UniProtKB/TrEMBL;Acc:Q20774])

HSP 1 Score: 145.976 bits (367), Expect = 1.062e-39
Identity = 105/333 (31.53%), Postives = 169/333 (50.75%), Query Frame = 1
Query:  139 CGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGG--------------GSSMDPFSSFFG--DVF-------FDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMK-----TIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFP 1053
             G+D+YK+L + + A  ++IKKAYR +A + HPDKNK +  AE++F+++  AY+ LSD +K+KI+D  GEEGLK+   G              G  M+ FSSFFG  D F       FD+GG         GG    ++F  + +    + +    ++  ++  H  +     H++       ++    +  +T++ + D    +    E+K+L V I+PG + G    F  EG+ H +  P D+ F I+   HP+F+R G D+     ISL  AL G  + I  LDG   ++    V  PG T + + +G+PN ++   +G L I  DV FP
Sbjct:    1 MGKDYYKVLGISKGATDDEIKKAYRKMALKYHPDKNK-EAGAENKFKEIAEAYDVLSDDKKKKIYDQFGEEGLKEGGPGAGGGGGGGMHYEFRGDPMNIFSSFFGGSDPFGAGGPGMFDLGGG-------AGGP---NMFF-MNQGGMDDGMFGGMHQGGRR-GHARQDPAVLHDLSVSLEDVLKGTTKKMKITRKVMTDNAQRL----EDKVLTVTIKPGWKSGTKITFPKEGDQHPNRTPADIVFVIKDKPHPKFKREGSDIKRVEKISLKSALTGLDIMIPTLDGADYRLQLNDVIKPGTTRRLTGKGLPNPKSPSHRGDLIIEFDVEFP 316          
BLAST of DnaJ-like protein vs. Ensembl Celegans
Match: dnj-13 (DNaJ domain (Prokaryotic heat shock protein) [Source:UniProtKB/TrEMBL;Acc:Q20774])

HSP 1 Score: 145.976 bits (367), Expect = 1.062e-39
Identity = 105/333 (31.53%), Postives = 169/333 (50.75%), Query Frame = 1
Query:  139 CGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGG--------------GSSMDPFSSFFG--DVF-------FDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMK-----TIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFP 1053
             G+D+YK+L + + A  ++IKKAYR +A + HPDKNK +  AE++F+++  AY+ LSD +K+KI+D  GEEGLK+   G              G  M+ FSSFFG  D F       FD+GG         GG    ++F  + +    + +    ++  ++  H  +     H++       ++    +  +T++ + D    +    E+K+L V I+PG + G    F  EG+ H +  P D+ F I+   HP+F+R G D+     ISL  AL G  + I  LDG   ++    V  PG T + + +G+PN ++   +G L I  DV FP
Sbjct:    1 MGKDYYKVLGISKGATDDEIKKAYRKMALKYHPDKNK-EAGAENKFKEIAEAYDVLSDDKKKKIYDQFGEEGLKEGGPGAGGGGGGGMHYEFRGDPMNIFSSFFGGSDPFGAGGPGMFDLGGG-------AGGP---NMFF-MNQGGMDDGMFGGMHQGGRR-GHARQDPAVLHDLSVSLEDVLKGTTKKMKITRKVMTDNAQRL----EDKVLTVTIKPGWKSGTKITFPKEGDQHPNRTPADIVFVIKDKPHPKFKREGSDIKRVEKISLKSALTGLDIMIPTLDGADYRLQLNDVIKPGTTRRLTGKGLPNPKSPSHRGDLIIEFDVEFP 316          
BLAST of DnaJ-like protein vs. Ensembl Celegans
Match: dnj-19 (DNaJ domain (Prokaryotic heat shock protein) [Source:UniProtKB/TrEMBL;Acc:O16303])

HSP 1 Score: 135.191 bits (339), Expect = 7.497e-35
Identity = 117/357 (32.77%), Postives = 169/357 (47.34%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKDIP------------------------RGGDVVMDLFVSLEELYNGNFVEVVRYK-SVKKSAHGT-------RRCN-CRHE-MKTI--QTGPGRFTMTQEQV---------------CDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEG-EPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREK--VTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKD 1059
             Y  LNV   A+   IKK+Y  LAK+ HPDKN   P+   +F++++ AYE LS PEKR+++DARG EG++    GG         F   F   GG     D                          +  D V  L V+LEELY G   ++   K ++ K+  G+        +C+ CR   +KTI  Q GPG     Q                  C  C   ++    K+LEV + PGM+      F  +G +   DGEPGD+   I+Q  H  F+R GDDL+    +SL +AL G++  IKHLDGH + +  ++  V  PG       +GMPN +   +KG+L++  +V FPK+
Sbjct:   14 LYTTLNVRPDASQADIKKSYFKLAKEYHPDKN---PDHGDKFKEISFAYEVLSSPEKRRLYDARGLEGVQGGGAGGGGGGFPGGLFSHFFGGAGGDDDDDDDDMGGHPFGGLFGGMGGMGRGGPRRRKFQDTVHPLNVTLEELYVGKTSKLKLSKKALCKTCEGSGGKKGEKYKCDACRGRGVKTIVQQIGPGMLQQMQVHCDACKGSGGKVPAGDKCKGCHGEKYENVSKILEVHVLPGMKHNDKITFKGDGDQSDPDGEPGDVVIVIQQKDHDIFKRDGDDLHMTKKLSLNEALCGYNFLIKHLDGHPLVLSSKQGDVIKPGVIRGVLGKGMPNKKYPELKGNLFVEFEVEFPKE 367          
BLAST of DnaJ-like protein vs. Ensembl Celegans
Match: dnj-19 (DNaJ domain (Prokaryotic heat shock protein) [Source:UniProtKB/TrEMBL;Acc:O16303])

HSP 1 Score: 135.191 bits (339), Expect = 7.497e-35
Identity = 117/357 (32.77%), Postives = 169/357 (47.34%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKDIP------------------------RGGDVVMDLFVSLEELYNGNFVEVVRYK-SVKKSAHGT-------RRCN-CRHE-MKTI--QTGPGRFTMTQEQV---------------CDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEG-EPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREK--VTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKD 1059
             Y  LNV   A+   IKK+Y  LAK+ HPDKN   P+   +F++++ AYE LS PEKR+++DARG EG++    GG         F   F   GG     D                          +  D V  L V+LEELY G   ++   K ++ K+  G+        +C+ CR   +KTI  Q GPG     Q                  C  C   ++    K+LEV + PGM+      F  +G +   DGEPGD+   I+Q  H  F+R GDDL+    +SL +AL G++  IKHLDGH + +  ++  V  PG       +GMPN +   +KG+L++  +V FPK+
Sbjct:   14 LYTTLNVRPDASQADIKKSYFKLAKEYHPDKN---PDHGDKFKEISFAYEVLSSPEKRRLYDARGLEGVQGGGAGGGGGGFPGGLFSHFFGGAGGDDDDDDDDMGGHPFGGLFGGMGGMGRGGPRRRKFQDTVHPLNVTLEELYVGKTSKLKLSKKALCKTCEGSGGKKGEKYKCDACRGRGVKTIVQQIGPGMLQQMQVHCDACKGSGGKVPAGDKCKGCHGEKYENVSKILEVHVLPGMKHNDKITFKGDGDQSDPDGEPGDVVIVIQQKDHDIFKRDGDDLHMTKKLSLNEALCGYNFLIKHLDGHPLVLSSKQGDVIKPGVIRGVLGKGMPNKKYPELKGNLFVEFEVEFPKE 367          
BLAST of DnaJ-like protein vs. Ensembl Fly
Match: shv (gene:FBgn0031256 transcript:FBtr0078060)

HSP 1 Score: 419.468 bits (1077), Expect = 3.661e-145
Identity = 225/359 (62.67%), Postives = 270/359 (75.21%), Query Frame = 1
Query:   79 FALMNFLIILLLSFA-VYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDS----HGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGL 1140
              L+  L+I+ LS   V   + GRDFYKILNV ++AN+N++KKAYR LAK+LHPDKNKDDP+A ++FQDL AAYE LS+P+KRK +D  GEE LK      HGG    DPFSSFFGD  F  GG  +Q+D PRG D+VMDL+VSLEELY+GNFVE+VR K V K A GTR+CNCR EM T   GPGRF M Q+ VCDECPNV+ V EE+ LE+E+E GM DGQ   FVAEGEPHIDGEPGDL  R++Q  HPRF R+ DDLYTNVTISL DAL GFSMEIKHLDGH V V REKVTWPGA I+K  EGMPN+ENN++ G+LYIT DV FPK  +  E+++ + K L  + ++ + YNGL
Sbjct:    1 MQLIKCLVIIQLSLLLVEESFAGRDFYKILNVKKNANTNEVKKAYRRLAKELHPDKNKDDPDASTKFQDLGAAYEVLSNPDKRKTYDRCGEECLKKEGMMDHGG----DPFSSFFGDFGFHFGGDGQQQDAPRGADIVMDLYVSLEELYSGNFVEIVRNKPVTKPASGTRKCNCRQEMVTRNLGPGRFQMIQQTVCDECPNVKLVNEERTLEIEVEQGMVDGQETRFVAEGEPHIDGEPGDLIVRVQQMPHPRFLRKNDDLYTNVTISLQDALVGFSMEIKHLDGHLVPVTREKVTWPGARIRKKGEGMPNFENNNLTGNLYITFDVEFPKKDLTEEDKEALKKILDQSSINRI-YNGL 354          
BLAST of DnaJ-like protein vs. Ensembl Fly
Match: Droj2 (gene:FBgn0038145 transcript:FBtr0082751)

HSP 1 Score: 147.132 bits (370), Expect = 2.053e-39
Identity = 115/339 (33.92%), Postives = 162/339 (47.79%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMD---PF-SSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSV---KKSAHGTRR------CNCRH---EMKTIQTGPGRF---------------TMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSM------EGMPNYENNHVKGSLYITIDVAFPK 1056
            +Y IL V  +A  +++KKAYR LA + HPDKN   PN   +F+ ++ AYE LSD +KR+++D  GE  +K   GG  S D   P             G    ++   RG DVV  + V LEELYNG   ++   K+V   K    G ++        CR    E +  Q  PG                 T+ ++  C  C   + V E K+LEV IE GMRDGQ   F  EG+   + +PGD+   + +  H  F   G DL   + + L++AL GF   +K LD   + V     T PG  I+  M      EGMP ++N   KG+L I  +V FP+
Sbjct:    7 YYDILGVKPNATPDELKKAYRKLALKYHPDKN---PNEGEKFKAISQAYEVLSDADKRQVYDEGGEAAIK--KGGADSGDFRNPMDFFEKFFGAGFGGSGGGRRRERRGKDVVHQMSVQLEELYNGATRKLQLQKNVICDKCEGRGGKKGSIEKCLQCRGNGVETRVQQIAPGIMQHIEQVCRKCSGTGETIQEKDRCKNCSGRKTVRERKVLEVHIEKGMRDGQKIVFTGEGDHEPESQPGDIIILLDEKEHSTFAHAGQDLMMKMPLQLVEALCGFQRIVKTLDDRDLIV----STQPGEVIRHEMTKCIAEEGMPIFKNPMEKGTLIIQFEVIFPE 336          
BLAST of DnaJ-like protein vs. Ensembl Fly
Match: Droj2 (gene:FBgn0038145 transcript:FBtr0082754)

HSP 1 Score: 147.132 bits (370), Expect = 2.053e-39
Identity = 115/339 (33.92%), Postives = 162/339 (47.79%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMD---PF-SSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSV---KKSAHGTRR------CNCRH---EMKTIQTGPGRF---------------TMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSM------EGMPNYENNHVKGSLYITIDVAFPK 1056
            +Y IL V  +A  +++KKAYR LA + HPDKN   PN   +F+ ++ AYE LSD +KR+++D  GE  +K   GG  S D   P             G    ++   RG DVV  + V LEELYNG   ++   K+V   K    G ++        CR    E +  Q  PG                 T+ ++  C  C   + V E K+LEV IE GMRDGQ   F  EG+   + +PGD+   + +  H  F   G DL   + + L++AL GF   +K LD   + V     T PG  I+  M      EGMP ++N   KG+L I  +V FP+
Sbjct:    7 YYDILGVKPNATPDELKKAYRKLALKYHPDKN---PNEGEKFKAISQAYEVLSDADKRQVYDEGGEAAIK--KGGADSGDFRNPMDFFEKFFGAGFGGSGGGRRRERRGKDVVHQMSVQLEELYNGATRKLQLQKNVICDKCEGRGGKKGSIEKCLQCRGNGVETRVQQIAPGIMQHIEQVCRKCSGTGETIQEKDRCKNCSGRKTVRERKVLEVHIEKGMRDGQKIVFTGEGDHEPESQPGDIIILLDEKEHSTFAHAGQDLMMKMPLQLVEALCGFQRIVKTLDDRDLIV----STQPGEVIRHEMTKCIAEEGMPIFKNPMEKGTLIIQFEVIFPE 336          
BLAST of DnaJ-like protein vs. Ensembl Fly
Match: Droj2 (gene:FBgn0038145 transcript:FBtr0082752)

HSP 1 Score: 147.132 bits (370), Expect = 2.053e-39
Identity = 115/339 (33.92%), Postives = 162/339 (47.79%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMD---PF-SSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSV---KKSAHGTRR------CNCRH---EMKTIQTGPGRF---------------TMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSM------EGMPNYENNHVKGSLYITIDVAFPK 1056
            +Y IL V  +A  +++KKAYR LA + HPDKN   PN   +F+ ++ AYE LSD +KR+++D  GE  +K   GG  S D   P             G    ++   RG DVV  + V LEELYNG   ++   K+V   K    G ++        CR    E +  Q  PG                 T+ ++  C  C   + V E K+LEV IE GMRDGQ   F  EG+   + +PGD+   + +  H  F   G DL   + + L++AL GF   +K LD   + V     T PG  I+  M      EGMP ++N   KG+L I  +V FP+
Sbjct:    7 YYDILGVKPNATPDELKKAYRKLALKYHPDKN---PNEGEKFKAISQAYEVLSDADKRQVYDEGGEAAIK--KGGADSGDFRNPMDFFEKFFGAGFGGSGGGRRRERRGKDVVHQMSVQLEELYNGATRKLQLQKNVICDKCEGRGGKKGSIEKCLQCRGNGVETRVQQIAPGIMQHIEQVCRKCSGTGETIQEKDRCKNCSGRKTVRERKVLEVHIEKGMRDGQKIVFTGEGDHEPESQPGDIIILLDEKEHSTFAHAGQDLMMKMPLQLVEALCGFQRIVKTLDDRDLIV----STQPGEVIRHEMTKCIAEEGMPIFKNPMEKGTLIIQFEVIFPE 336          
BLAST of DnaJ-like protein vs. Ensembl Fly
Match: Droj2 (gene:FBgn0038145 transcript:FBtr0082753)

HSP 1 Score: 147.132 bits (370), Expect = 2.053e-39
Identity = 115/339 (33.92%), Postives = 162/339 (47.79%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMD---PF-SSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSV---KKSAHGTRR------CNCRH---EMKTIQTGPGRF---------------TMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSM------EGMPNYENNHVKGSLYITIDVAFPK 1056
            +Y IL V  +A  +++KKAYR LA + HPDKN   PN   +F+ ++ AYE LSD +KR+++D  GE  +K   GG  S D   P             G    ++   RG DVV  + V LEELYNG   ++   K+V   K    G ++        CR    E +  Q  PG                 T+ ++  C  C   + V E K+LEV IE GMRDGQ   F  EG+   + +PGD+   + +  H  F   G DL   + + L++AL GF   +K LD   + V     T PG  I+  M      EGMP ++N   KG+L I  +V FP+
Sbjct:    7 YYDILGVKPNATPDELKKAYRKLALKYHPDKN---PNEGEKFKAISQAYEVLSDADKRQVYDEGGEAAIK--KGGADSGDFRNPMDFFEKFFGAGFGGSGGGRRRERRGKDVVHQMSVQLEELYNGATRKLQLQKNVICDKCEGRGGKKGSIEKCLQCRGNGVETRVQQIAPGIMQHIEQVCRKCSGTGETIQEKDRCKNCSGRKTVRERKVLEVHIEKGMRDGQKIVFTGEGDHEPESQPGDIIILLDEKEHSTFAHAGQDLMMKMPLQLVEALCGFQRIVKTLDDRDLIV----STQPGEVIRHEMTKCIAEEGMPIFKNPMEKGTLIIQFEVIFPE 336          
BLAST of DnaJ-like protein vs. Ensembl Zebrafish
Match: dnajb11 (DnaJ heat shock protein family (Hsp40) member B11 [Source:ZFIN;Acc:ZDB-GENE-031113-9])

HSP 1 Score: 405.216 bits (1040), Expect = 2.085e-139
Identity = 209/353 (59.21%), Postives = 259/353 (73.37%), Query Frame = 1
Query:  100 IILLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQ---KDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGLGGY 1149
            +  LL + +  ++ GRDFYKIL V RSA+   IKKAYR LA QLHPD+N+DDPNA+ +F DL AAYE LSD EKRK +DA GEEGLK+ H   SS     S F   F  M G  RQ   +DIPRG D+V+DL V+LEE+Y+GNFVEVVR K V K A G R+CNCR EM+T Q GPGRF MTQE VCDECPN++ V EE+ LEVEIE G+RD   YPF+ EGEPHIDGEPGDL+FRI+   HP FERRGDDLYTNVTISL++AL GF M+I HLDGHKV ++R+K+T PG+ I K  EG+P+++NN+++GSL IT DV FPK+++  +++  I + L       + YNGL GY
Sbjct:   11 VCFLLLYLILTVFAGRDFYKILGVSRSASVKDIKKAYRKLALQLHPDRNQDDPNAQDKFADLGAAYEVLSDEEKRKQYDAYGEEGLKEGHQ--SSHGDIFSSFFGDFGFMFGGNRQPAGRDIPRGNDIVLDLEVTLEEVYSGNFVEVVRIKPVAKEAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNIKLVNEERTLEVEIEQGVRDEMEYPFIGEGEPHIDGEPGDLRFRIKVLKHPVFERRGDDLYTNVTISLVEALVGFEMDITHLDGHKVHIVRDKITKPGSRIWKKGEGLPSFDNNNIRGSLIITFDVDFPKEQLDDQQKDGIKQLLKQAPSQKV-YNGLQGY 360          
BLAST of DnaJ-like protein vs. Ensembl Zebrafish
Match: dnaja2a (DnaJ heat shock protein family (Hsp40) member A2a [Source:ZFIN;Acc:ZDB-GENE-040426-2884])

HSP 1 Score: 169.859 bits (429), Expect = 1.680e-47
Identity = 118/340 (34.71%), Postives = 171/340 (50.29%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGG----DVVMDLFVSLEELYNGNFVEVVRYKSVKKS--------AHGTRRCN-CRHEMKTI---QTGPGRF---------------TMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHK--VKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYS 1071
             Y IL V  SA+ N++KKAYR LAK+ HPDKN   PNA  +F++++ AYE L++PEKR ++D  GE+GL++   GG  MD   S             + +    G     D+V  L VSLE+LYNG   ++   K+V  S        +   ++C  CR     I   Q GPG                  ++++  C +C   + + E K+LEV ++ GM+ GQ   F  E +     EPGD+   +++  H  + R G+DL+    I L++AL GF   +KHLDG +  VK    K+  PG+      EGMP Y N   KG L+I  DV FP +   S
Sbjct:    9 LYDILGVSPSASENELKKAYRKLAKEYHPDKN---PNAGDKFKEISFAYEVLTNPEKRDMYDRYGEQGLREGGCGGGGMDDIFSHIFGGGLFGFMGGQGRSRNGGRRRGEDMVHPLKVSLEDLYNGKTTKLQLSKNVLCSTCNGQGGKSGAVQKCTACRGRGMRIMIRQLGPGMVQQMQSVCTDCNGEGEVISEKDRCKKCEGKKVIKEVKILEVHVDKGMKHGQKITFGGEADQSPGVEPGDIVLVLQEKEHETYRREGNDLHMTHKIGLVEALCGFHFTLKHLDGRQIVVKYPAGKIIEPGSVRVVRGEGMPQYRNPFEKGDLFIKFDVQFPDNNWLS 345          
BLAST of DnaJ-like protein vs. Ensembl Zebrafish
Match: dnaja1 (DnaJ heat shock protein family (Hsp40) member A1 [Source:ZFIN;Acc:ZDB-GENE-030131-2642])

HSP 1 Score: 163.31 bits (412), Expect = 2.660e-45
Identity = 116/338 (34.32%), Postives = 171/338 (50.59%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSS--MDPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSV---KKSAHGTRR-----CN-CRH---EMKTIQTGPGRFT---------------MTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKS------MEGMPNYENNHVKGSLYITIDVAFPKD 1059
            FY +L V  SA+  ++KKAYR LA + HPDKN   P    +F+ ++ AYE LSD +KR+++D  GE+ +K+   GGS   MD F  FFG      GG R  ++  RG +VV  L VSLE+LYNG   ++   K+V   K    G R+     C  CR    +++     PG                  +     C  C   + + ++K+LEV I+ GM+DGQ   F  EG+     +PGD+   + Q +HP + R+GDDL  ++ + L+++L GF   IK LD   + +     + PG  IK         EGMP +     KG L I  +V FP++
Sbjct:    7 FYDMLGVKPSASPEELKKAYRKLALKYHPDKN---PTEGEKFKQISQAYEVLSDAKKREVYDRGGEKAIKEGGNGGSCSPMDIFDLFFG------GGGRMHRE-RRGKNVVHQLTVSLEDLYNGTTRKLALQKNVICDKCEGRGGRKGVIEVCPLCRGVGVQVRLHHLAPGMVQQISTVCEGCQGQGQRLGHRDRCKTCTGRKILRQKKILEVHIDKGMKDGQKIVFHGEGDQEPGLKPGDIIIVLDQRAHPLYTRQGDDLIVSMELQLVESLCGFQKPIKTLDSRTLLI----TSHPGELIKPGDKKCVMNEGMPMHRRPFEKGKLIIHFNVVFPEE 330          
BLAST of DnaJ-like protein vs. Ensembl Zebrafish
Match: dnaja2b (DnaJ heat shock protein family (Hsp40) member A2b [Source:ZFIN;Acc:ZDB-GENE-030131-2884])

HSP 1 Score: 155.221 bits (391), Expect = 4.546e-42
Identity = 118/337 (35.01%), Postives = 166/337 (49.26%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDA-RGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKDIP----RGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSA--------HGTRRCN-CRHEMKTI---QTGPGRFTMTQEQV---------------CDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHK--VKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKD 1059
             Y +L V  SA+ N++KKAYR LAK+ HPDKN   PNA  +F++++ AYE L++PEK+ ++D    +   +   GG    D FS  FG   F   G +  K       RG D++  L VSLE+LYNG   ++   K+V  SA           ++C+ CR     I   Q  PG     Q                  C EC   +   E K+LEV ++ GM+ GQ   F  E +   + EPGD+   +++  H  F R G+DL+    I L++AL GF   + HLDG    +K    KV  PG+      EGMP Y N   KG L+I  DV FP++
Sbjct:    9 LYDLLGVSPSASENELKKAYRKLAKEYHPDKN---PNAGDKFKEISFAYEVLTNPEKKDLYDRYGEQGLREGGGGGAGMEDIFSHIFGGGLFGFMGGQSSKSRNGGRRRGEDMIHPLKVSLEDLYNGKTTKLQLSKNVLCSACNGQGGKTGAVQKCSTCRGRGMRIMIRQLAPGMVQQMQSVCTDCNGEGEVIHEKDRCKECDGRKVCKEVKVLEVHVDKGMKHGQKITFSGEADQSPNTEPGDIILVLQEKDHEEFRRDGNDLHIGHKIGLVEALCGFQFMLTHLDGRHLVIKYPPGKVIEPGSIRVVRGEGMPQYRNPFEKGDLFIKFDVQFPEN 342          
BLAST of DnaJ-like protein vs. Ensembl Zebrafish
Match: CABZ01080568.1 (pep chromosome:GRCz11:25:34662358:34670413:-1 gene:ENSDARG00000051762.6 transcript:ENSDART00000073440.6 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CABZ01080568.1)

HSP 1 Score: 149.058 bits (375), Expect = 4.185e-40
Identity = 113/323 (34.98%), Postives = 167/323 (51.70%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDS--HGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCN--CRHEMKTIQTGPGR--FTMTQEQV--------CDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKS------MEGMPNYENNHVKGSLYITIDVAFPKD 1059
            +Y  L V   A+ ++IKKAYR LA + HPDKN   PN   +F+ ++ AY+ LSD +KR+++D  GE+ +K+    GG S MD F+ FFG      GG R Q++  +G ++V  L V+LEELYNG+  ++   K+V       ++C+  C ++ K+I  G  R  F     QV        C +   V F     L+ + I  GM+DGQ   F  EG+     EPGD+   +    HP F+R+ ++L   + I L++AL GF   I  LD   + +     + PG  IK +       EGMP Y     KG L I  ++ FP D
Sbjct:    8 YYDQLGVKPDASLDEIKKAYRKLALKYHPDKN---PNEGEKFKLISQAYDVLSDAKKRELYDQGGEQAIKEGGMAGGDSPMDIFNMFFG------GGGRMQRE-RKGKNLVHQLGVTLEELYNGSTRKLALQKNVI-----CQKCDGVCNNDGKSIHVGLLRLGFVEIVRQVSLKSSGLDCAKISGVSFF-SSVLIMLSI-AGMKDGQKITFNGEGDQEPGLEPGDVTIILDLKEHPVFQRKDNNLLMKMKIRLVEALCGFKKTISTLDNRSLLI----HSPPGQVIKPNDLKCVHNEGMPVYREPFEKGLLIIRFEIEFPDD 309          
BLAST of DnaJ-like protein vs. Ensembl Xenopus
Match: dnajb11 (DnaJ heat shock protein family (Hsp40) member B11 [Source:NCBI gene;Acc:394787])

HSP 1 Score: 408.683 bits (1049), Expect = 1.156e-140
Identity = 210/352 (59.66%), Postives = 263/352 (74.72%), Query Frame = 1
Query:  100 IILLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKD--IPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGLGGY 1149
            +  L+ + +  +  GRDFYKIL V + A   +IKKAYR LA QLHPD+N DDPNA+ +FQDL AAYE LSD EKRK +D  GEEGLKD H   S  D FS FFGD  F  GG+ RQ+D  IPRG D+++DL V+LEE+Y+GNFVEV+R K V + A G R+CNCR EM+T Q GPGRF MTQE VCDECPNV+ V EE+ LEVEIEPG+RD   YPF+ EGEPHIDGEPGDL+FRI+   HP FERRGDDLYTNV+ISL++AL GF M+I HLDGHKV ++R+K+T PGA + K  EG+PN++NN++KGSL IT DV FPK+++  E+ + + + +    +  + YNGL GY
Sbjct:   11 VCFLIFYLMVIVSGGRDFYKILGVSKGATVKEIKKAYRKLALQLHPDRNPDDPNAQEKFQDLGAAYEVLSDEEKRKQYDTYGEEGLKDGHQS-SHGDIFSHFFGDFGFMFGGNPRQQDRNIPRGSDIIVDLEVTLEEVYSGNFVEVIRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDSMEYPFIGEGEPHIDGEPGDLRFRIKVLKHPIFERRGDDLYTNVSISLVEALTGFEMDIAHLDGHKVHILRDKITKPGAKLWKKGEGLPNFDNNNIKGSLIITFDVEFPKEQLTMEQRQGVKQLMKQNSIQRI-YNGLQGY 360          
BLAST of DnaJ-like protein vs. Ensembl Xenopus
Match: dnajb11 (DnaJ heat shock protein family (Hsp40) member B11 [Source:NCBI gene;Acc:394787])

HSP 1 Score: 404.831 bits (1039), Expect = 6.077e-139
Identity = 203/324 (62.65%), Postives = 249/324 (76.85%), Query Frame = 1
Query:  100 IILLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKD--IPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKV 1065
            +  L+ + +  +  GRDFYKIL V + A   +IKKAYR LA QLHPD+N DDPNA+ +FQDL AAYE LSD EKRK +D  GEEGLKD H   S  D FS FFGD  F  GG+ RQ+D  IPRG D+++DL V+LEE+Y+GNFVEV+R K V + A G R+CNCR EM+T Q GPGRF MTQE VCDECPNV+ V EE+ LEVEIEPG+RD   YPF+ EGEPHIDGEPGDL+FRI+   HP FERRGDDLYTNV+ISL++AL GF M+I HLDGHKV ++R+K+T PGA + K  EG+PN++NN++KGSL IT DV FPK+++
Sbjct:   11 VCFLIFYLMVIVSGGRDFYKILGVSKGATVKEIKKAYRKLALQLHPDRNPDDPNAQEKFQDLGAAYEVLSDEEKRKQYDTYGEEGLKDGHQS-SHGDIFSHFFGDFGFMFGGNPRQQDRNIPRGSDIIVDLEVTLEEVYSGNFVEVIRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDSMEYPFIGEGEPHIDGEPGDLRFRIKVLKHPIFERRGDDLYTNVSISLVEALTGFEMDIAHLDGHKVHILRDKITKPGAKLWKKGEGLPNFDNNNIKGSLIITFDVEFPKEQL 333          
BLAST of DnaJ-like protein vs. Ensembl Xenopus
Match: dnajb11 (DnaJ heat shock protein family (Hsp40) member B11 [Source:NCBI gene;Acc:394787])

HSP 1 Score: 400.979 bits (1029), Expect = 6.074e-138
Identity = 202/310 (65.16%), Postives = 243/310 (78.39%), Query Frame = 1
Query:  142 GRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKD--IPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKV 1065
            GRDFYKIL V + A   +IKKAYR LA QLHPD+N DDPNA+ +FQDL AAYE LSD EKRK +D  GEEGLKD H   S  D FS FFGD  F  GG+ RQ+D  IPRG D+++DL V+LEE+Y+GNFVEV+R K V + A G R+CNCR EM+T Q GPGRF MTQE VCDECPNV+ V EE+ LEVEIEPG+RD   YPF+ EGEPHIDGEPGDL+FRI+   HP FERRGDDLYTNV+ISL++AL GF M+I HLDGHKV ++R+K+T PGA + K  EG+PN++NN++KGSL IT DV FPK+++
Sbjct:    7 GRDFYKILGVSKGATVKEIKKAYRKLALQLHPDRNPDDPNAQEKFQDLGAAYEVLSDEEKRKQYDTYGEEGLKDGHQS-SHGDIFSHFFGDFGFMFGGNPRQQDRNIPRGSDIIVDLEVTLEEVYSGNFVEVIRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDSMEYPFIGEGEPHIDGEPGDLRFRIKVLKHPIFERRGDDLYTNVSISLVEALTGFEMDIAHLDGHKVHILRDKITKPGAKLWKKGEGLPNFDNNNIKGSLIITFDVEFPKEQL 315          
BLAST of DnaJ-like protein vs. Ensembl Xenopus
Match: dnajb11 (DnaJ heat shock protein family (Hsp40) member B11 [Source:NCBI gene;Acc:394787])

HSP 1 Score: 400.208 bits (1027), Expect = 1.300e-137
Identity = 201/317 (63.41%), Postives = 245/317 (77.29%), Query Frame = 1
Query:  121 AVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKD--IPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKV 1065
             ++  +  RDFYKIL V + A   +IKKAYR LA QLHPD+N DDPNA+ +FQDL AAYE LSD EKRK +D  GEEGLKD H   S  D FS FFGD  F  GG+ RQ+D  IPRG D+++DL V+LEE+Y+GNFVEV+R K V + A G R+CNCR EM+T Q GPGRF MTQE VCDECPNV+ V EE+ LEVEIEPG+RD   YPF+ EGEPHIDGEPGDL+FRI+   HP FERRGDDLYTNV+ISL++AL GF M+I HLDGHKV ++R+K+T PGA + K  EG+PN++NN++KGSL IT DV FPK+++
Sbjct:    1 VIFFTFSLRDFYKILGVSKGATVKEIKKAYRKLALQLHPDRNPDDPNAQEKFQDLGAAYEVLSDEEKRKQYDTYGEEGLKDGHQS-SHGDIFSHFFGDFGFMFGGNPRQQDRNIPRGSDIIVDLEVTLEEVYSGNFVEVIRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDSMEYPFIGEGEPHIDGEPGDLRFRIKVLKHPIFERRGDDLYTNVSISLVEALTGFEMDIAHLDGHKVHILRDKITKPGAKLWKKGEGLPNFDNNNIKGSLIITFDVEFPKEQL 316          
BLAST of DnaJ-like protein vs. Ensembl Xenopus
Match: dnajb11 (DnaJ heat shock protein family (Hsp40) member B11 [Source:NCBI gene;Acc:394787])

HSP 1 Score: 399.438 bits (1025), Expect = 3.591e-137
Identity = 201/309 (65.05%), Postives = 242/309 (78.32%), Query Frame = 1
Query:  145 RDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKD--IPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKV 1065
            RDFYKIL V + A   +IKKAYR LA QLHPD+N DDPNA+ +FQDL AAYE LSD EKRK +D  GEEGLKD H   S  D FS FFGD  F  GG+ RQ+D  IPRG D+++DL V+LEE+Y+GNFVEV+R K V + A G R+CNCR EM+T Q GPGRF MTQE VCDECPNV+ V EE+ LEVEIEPG+RD   YPF+ EGEPHIDGEPGDL+FRI+   HP FERRGDDLYTNV+ISL++AL GF M+I HLDGHKV ++R+K+T PGA + K  EG+PN++NN++KGSL IT DV FPK+++
Sbjct:   16 RDFYKILGVSKGATVKEIKKAYRKLALQLHPDRNPDDPNAQEKFQDLGAAYEVLSDEEKRKQYDTYGEEGLKDGHQS-SHGDIFSHFFGDFGFMFGGNPRQQDRNIPRGSDIIVDLEVTLEEVYSGNFVEVIRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDSMEYPFIGEGEPHIDGEPGDLRFRIKVLKHPIFERRGDDLYTNVSISLVEALTGFEMDIAHLDGHKVHILRDKITKPGAKLWKKGEGLPNFDNNNIKGSLIITFDVEFPKEQL 323          
BLAST of DnaJ-like protein vs. Ensembl Mouse
Match: Dnajb11 (DnaJ heat shock protein family (Hsp40) member B11 [Source:MGI Symbol;Acc:MGI:1915088])

HSP 1 Score: 410.994 bits (1055), Expect = 9.974e-142
Identity = 216/350 (61.71%), Postives = 262/350 (74.86%), Query Frame = 1
Query:  106 LLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKD--IPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGLGGY 1149
            LLL + +  +  GRDFYKIL VPRSA+   IKKAYR LA QLHPD+N DDP A+ +FQDL AAYE LSD EKRK +D  GEEGLKD H   S  D FS FFGD  F  GG+ RQ+D  IPRG D+++DL V+LEE+Y GNFVEVVR K V + A G R+CNCR EM+T Q GPGRF MTQE VCDECPNV+ V EE+ LEVEIEPG+RDG  YPF+ EGEPH+DGEPGDL+FRI+   H  FERRGDDLYTNVT+SL++AL GF M+I HLDGHKV + R+K+T PGA + K  EG+PN++NN++KGSL IT DV FPK+++  E ++ I + L    +  + YNGL GY
Sbjct:   11 LLLLYLIGTVIAGRDFYKILGVPRSASIKDIKKAYRKLALQLHPDRNPDDPQAQEKFQDLGAAYEVLSDSEKRKQYDTYGEEGLKDGHQS-SHGDIFSHFFGDFGFMFGGTPRQQDRNIPRGSDIIVDLEVTLEEVYAGNFVEVVRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHRIFERRGDDLYTNVTVSLVEALVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAKEGIKQLLKQGPVQKV-YNGLQGY 358          
BLAST of DnaJ-like protein vs. Ensembl Mouse
Match: Dnajb11 (DnaJ heat shock protein family (Hsp40) member B11 [Source:MGI Symbol;Acc:MGI:1915088])

HSP 1 Score: 410.994 bits (1055), Expect = 9.974e-142
Identity = 216/350 (61.71%), Postives = 262/350 (74.86%), Query Frame = 1
Query:  106 LLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKD--IPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGLGGY 1149
            LLL + +  +  GRDFYKIL VPRSA+   IKKAYR LA QLHPD+N DDP A+ +FQDL AAYE LSD EKRK +D  GEEGLKD H   S  D FS FFGD  F  GG+ RQ+D  IPRG D+++DL V+LEE+Y GNFVEVVR K V + A G R+CNCR EM+T Q GPGRF MTQE VCDECPNV+ V EE+ LEVEIEPG+RDG  YPF+ EGEPH+DGEPGDL+FRI+   H  FERRGDDLYTNVT+SL++AL GF M+I HLDGHKV + R+K+T PGA + K  EG+PN++NN++KGSL IT DV FPK+++  E ++ I + L    +  + YNGL GY
Sbjct:   11 LLLLYLIGTVIAGRDFYKILGVPRSASIKDIKKAYRKLALQLHPDRNPDDPQAQEKFQDLGAAYEVLSDSEKRKQYDTYGEEGLKDGHQS-SHGDIFSHFFGDFGFMFGGTPRQQDRNIPRGSDIIVDLEVTLEEVYAGNFVEVVRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHRIFERRGDDLYTNVTVSLVEALVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAKEGIKQLLKQGPVQKV-YNGLQGY 358          
BLAST of DnaJ-like protein vs. Ensembl Mouse
Match: Dnajb11 (DnaJ heat shock protein family (Hsp40) member B11 [Source:MGI Symbol;Acc:MGI:1915088])

HSP 1 Score: 410.994 bits (1055), Expect = 9.974e-142
Identity = 216/350 (61.71%), Postives = 262/350 (74.86%), Query Frame = 1
Query:  106 LLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKD--IPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGLGGY 1149
            LLL + +  +  GRDFYKIL VPRSA+   IKKAYR LA QLHPD+N DDP A+ +FQDL AAYE LSD EKRK +D  GEEGLKD H   S  D FS FFGD  F  GG+ RQ+D  IPRG D+++DL V+LEE+Y GNFVEVVR K V + A G R+CNCR EM+T Q GPGRF MTQE VCDECPNV+ V EE+ LEVEIEPG+RDG  YPF+ EGEPH+DGEPGDL+FRI+   H  FERRGDDLYTNVT+SL++AL GF M+I HLDGHKV + R+K+T PGA + K  EG+PN++NN++KGSL IT DV FPK+++  E ++ I + L    +  + YNGL GY
Sbjct:   11 LLLLYLIGTVIAGRDFYKILGVPRSASIKDIKKAYRKLALQLHPDRNPDDPQAQEKFQDLGAAYEVLSDSEKRKQYDTYGEEGLKDGHQS-SHGDIFSHFFGDFGFMFGGTPRQQDRNIPRGSDIIVDLEVTLEEVYAGNFVEVVRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHRIFERRGDDLYTNVTVSLVEALVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAKEGIKQLLKQGPVQKV-YNGLQGY 358          
BLAST of DnaJ-like protein vs. Ensembl Mouse
Match: Dnaja2 (DnaJ heat shock protein family (Hsp40) member A2 [Source:MGI Symbol;Acc:MGI:1931882])

HSP 1 Score: 173.326 bits (438), Expect = 9.066e-49
Identity = 120/335 (35.82%), Postives = 167/335 (49.85%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGG---DVVMDLFVSLEELYNGNFVEVVRYKSVKKSA--------HGTRRCN-CRHEMKTI---QTGPGRFTMTQEQV---------------CDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHK--VKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKD 1059
             Y IL VP  A+ N++KKAYR LAK+ HPDKN   PNA  +F++++ AYE LS+PEKR+++D  GE+GL++  GGG  MD   S             + +         D++  L VSLE+LYNG   ++   K+V  SA           ++C+ CR     I   Q  PG     Q                  C +C   + + E K+LEV ++ GM+ GQ   F  E +     EPGD+   +++  H  F+R G+DL+    I L++AL GF    KHLD  +  VK    KV  PG       EGMP Y N   KG LYI  DV FP++
Sbjct:    9 LYDILGVPPGASENELKKAYRKLAKEYHPDKN---PNAGDKFKEISFAYEVLSNPEKRELYDRYGEQGLREGSGGGGGMDDIFSHIFGGGLFGFMGNQSRSRNGRRRGEDMMHPLKVSLEDLYNGKTTKLQLSKNVLCSACSGQGGKSGAVQKCSACRGRGVRIMIRQLAPGMVQQMQSVCSDCNGEGEVINEKDRCKKCEGKKVIKEVKILEVHVDKGMKHGQRITFTGEADQAPGVEPGDIVLLLQEKEHEVFQRDGNDLHMTYKIGLVEALCGFQFTFKHLDARQIVVKYPPGKVIEPGCVRVVRGEGMPQYRNPFEKGDLYIKFDVQFPEN 340          
BLAST of DnaJ-like protein vs. Ensembl Mouse
Match: Dnaja1 (DnaJ heat shock protein family (Hsp40) member A1 [Source:MGI Symbol;Acc:MGI:1270129])

HSP 1 Score: 171.785 bits (434), Expect = 2.478e-48
Identity = 121/343 (35.28%), Postives = 171/343 (49.85%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGG---SSMDPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSV-------KKSAHGTRRC--NCR---HEMKTIQTGPGRFTMTQEQV---------------CDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKS------MEGMPNYENNHVKGSLYITIDVAFPKDKVYS 1071
            +Y +L V  +A   ++KKAYR LA + HPDKN   PN   +F+ ++ AYE L+D +KR+++D  GE+ +K+   GG   S MD F  FFG      GG R Q++  RG +VV  L V+LE+LYNG   ++   K+V       +    G   C  NCR    +++  Q GPG     Q                  C  C   + V E+K+LEV I+ GM+DGQ   F  EG+     EPGD+   + Q  H  F RRG+DL+  + I L++AL GF   I  LD   + +     + PG  +K         EGMP Y   + KG L I   V FP++   S
Sbjct:    7 YYDVLGVKPNATQEELKKAYRKLALKYHPDKN---PNEGEKFKQISQAYEVLADSKKRELYDKGGEQAIKEGGAGGGFGSPMDIFDMFFG------GGGRMQRE-RRGKNVVHQLSVTLEDLYNGATRKLALQKNVICDKCEGRGGKKGAVECCPNCRGTGMQIRIHQIGPGMVQQIQSVCMECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKDGQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVI----TSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLS 335          
BLAST of DnaJ-like protein vs. UniProt/SwissProt
Match: sp|Q9VPQ2|DJSHV_DROME (DnaJ homolog shv OS=Drosophila melanogaster OX=7227 GN=shv PE=1 SV=1)

HSP 1 Score: 419.468 bits (1077), Expect = 3.669e-144
Identity = 225/359 (62.67%), Postives = 270/359 (75.21%), Query Frame = 1
Query:   79 FALMNFLIILLLSFA-VYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDS----HGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGL 1140
              L+  L+I+ LS   V   + GRDFYKILNV ++AN+N++KKAYR LAK+LHPDKNKDDP+A ++FQDL AAYE LS+P+KRK +D  GEE LK      HGG    DPFSSFFGD  F  GG  +Q+D PRG D+VMDL+VSLEELY+GNFVE+VR K V K A GTR+CNCR EM T   GPGRF M Q+ VCDECPNV+ V EE+ LE+E+E GM DGQ   FVAEGEPHIDGEPGDL  R++Q  HPRF R+ DDLYTNVTISL DAL GFSMEIKHLDGH V V REKVTWPGA I+K  EGMPN+ENN++ G+LYIT DV FPK  +  E+++ + K L  + ++ + YNGL
Sbjct:    1 MQLIKCLVIIQLSLLLVEESFAGRDFYKILNVKKNANTNEVKKAYRRLAKELHPDKNKDDPDASTKFQDLGAAYEVLSNPDKRKTYDRCGEECLKKEGMMDHGG----DPFSSFFGDFGFHFGGDGQQQDAPRGADIVMDLYVSLEELYSGNFVEIVRNKPVTKPASGTRKCNCRQEMVTRNLGPGRFQMIQQTVCDECPNVKLVNEERTLEIEVEQGMVDGQETRFVAEGEPHIDGEPGDLIVRVQQMPHPRFLRKNDDLYTNVTISLQDALVGFSMEIKHLDGHLVPVTREKVTWPGARIRKKGEGMPNFENNNLTGNLYITFDVEFPKKDLTEEDKEALKKILDQSSINRI-YNGL 354          
BLAST of DnaJ-like protein vs. UniProt/SwissProt
Match: sp|Q5RAJ6|DJB11_PONAB (DnaJ homolog subfamily B member 11 OS=Pongo abelii OX=9601 GN=DNAJB11 PE=2 SV=1)

HSP 1 Score: 414.461 bits (1064), Expect = 3.409e-142
Identity = 217/350 (62.00%), Postives = 262/350 (74.86%), Query Frame = 1
Query:  106 LLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKD--IPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGLGGY 1149
            LLL + +  +  GRDFYKIL VPRSA+   IKKAYR LA QLHPD+N DDP A+ +FQDL AAYE LSD EKRK +D  GEEGLKD H   S  D FS FFGD  F  GG+ RQ+D  IPRG D+++DL V+LEE+Y GNFVEVVR K V + A G R+CNCR EM+T Q GPGRF MTQE VCDECPNV+ V EE+ LEVEIEPG+RDG  YPF+ EGEPH+DGEPGDL+FRI+   HP FERRGDDLYTNVTISL+++L GF M+I HLDGHKV + R+K+T PGA + K  EG+PN++NN++KGSL IT DV FPK+++  E  + I + L    +  + YNGL GY
Sbjct:   11 LLLLYLIGAVIAGRDFYKILGVPRSASIKDIKKAYRKLALQLHPDRNPDDPQAQEKFQDLGAAYEVLSDSEKRKQYDTYGEEGLKDGHQS-SHGDIFSHFFGDFGFMFGGTPRQQDRNIPRGSDIIVDLEVTLEEVYAGNFVEVVRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHPIFERRGDDLYTNVTISLVESLVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAREGIKQLLKQGSVQKV-YNGLQGY 358          
BLAST of DnaJ-like protein vs. UniProt/SwissProt
Match: sp|Q9UBS4|DJB11_HUMAN (DnaJ homolog subfamily B member 11 OS=Homo sapiens OX=9606 GN=DNAJB11 PE=1 SV=1)

HSP 1 Score: 414.461 bits (1064), Expect = 3.409e-142
Identity = 217/350 (62.00%), Postives = 262/350 (74.86%), Query Frame = 1
Query:  106 LLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKD--IPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGLGGY 1149
            LLL + +  +  GRDFYKIL VPRSA+   IKKAYR LA QLHPD+N DDP A+ +FQDL AAYE LSD EKRK +D  GEEGLKD H   S  D FS FFGD  F  GG+ RQ+D  IPRG D+++DL V+LEE+Y GNFVEVVR K V + A G R+CNCR EM+T Q GPGRF MTQE VCDECPNV+ V EE+ LEVEIEPG+RDG  YPF+ EGEPH+DGEPGDL+FRI+   HP FERRGDDLYTNVTISL+++L GF M+I HLDGHKV + R+K+T PGA + K  EG+PN++NN++KGSL IT DV FPK+++  E  + I + L    +  + YNGL GY
Sbjct:   11 LLLLYLIGAVIAGRDFYKILGVPRSASIKDIKKAYRKLALQLHPDRNPDDPQAQEKFQDLGAAYEVLSDSEKRKQYDTYGEEGLKDGHQS-SHGDIFSHFFGDFGFMFGGTPRQQDRNIPRGSDIIVDLEVTLEEVYAGNFVEVVRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHPIFERRGDDLYTNVTISLVESLVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAREGIKQLLKQGSVQKV-YNGLQGY 358          
BLAST of DnaJ-like protein vs. UniProt/SwissProt
Match: sp|Q99KV1|DJB11_MOUSE (DnaJ homolog subfamily B member 11 OS=Mus musculus OX=10090 GN=Dnajb11 PE=1 SV=1)

HSP 1 Score: 410.994 bits (1055), Expect = 6.977e-141
Identity = 216/350 (61.71%), Postives = 262/350 (74.86%), Query Frame = 1
Query:  106 LLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKD--IPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGLGGY 1149
            LLL + +  +  GRDFYKIL VPRSA+   IKKAYR LA QLHPD+N DDP A+ +FQDL AAYE LSD EKRK +D  GEEGLKD H   S  D FS FFGD  F  GG+ RQ+D  IPRG D+++DL V+LEE+Y GNFVEVVR K V + A G R+CNCR EM+T Q GPGRF MTQE VCDECPNV+ V EE+ LEVEIEPG+RDG  YPF+ EGEPH+DGEPGDL+FRI+   H  FERRGDDLYTNVT+SL++AL GF M+I HLDGHKV + R+K+T PGA + K  EG+PN++NN++KGSL IT DV FPK+++  E ++ I + L    +  + YNGL GY
Sbjct:   11 LLLLYLIGTVIAGRDFYKILGVPRSASIKDIKKAYRKLALQLHPDRNPDDPQAQEKFQDLGAAYEVLSDSEKRKQYDTYGEEGLKDGHQS-SHGDIFSHFFGDFGFMFGGTPRQQDRNIPRGSDIIVDLEVTLEEVYAGNFVEVVRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHRIFERRGDDLYTNVTVSLVEALVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAKEGIKQLLKQGPVQKV-YNGLQGY 358          
BLAST of DnaJ-like protein vs. UniProt/SwissProt
Match: sp|Q6TUG0|DJB11_RAT (DnaJ homolog subfamily B member 11 OS=Rattus norvegicus OX=10116 GN=Dnajb11 PE=2 SV=1)

HSP 1 Score: 410.994 bits (1055), Expect = 7.865e-141
Identity = 216/350 (61.71%), Postives = 262/350 (74.86%), Query Frame = 1
Query:  106 LLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKD--IPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGLGGY 1149
            LLL + +  +  GRDFYKIL VPRSA+   IKKAYR LA QLHPD+N DDP A+ +FQDL AAYE LSD EKRK +D  GEEGLKD H   S  D FS FFGD  F  GG+ RQ+D  IPRG D+++DL V+LEE+Y GNFVEVVR K V + A G R+CNCR EM+T Q GPGRF MTQE VCDECPNV+ V EE+ LEVEIEPG+RDG  YPF+ EGEPH+DGEPGDL+FRI+   H  FERRGDDLYTNVT+SL++AL GF M+I HLDGHKV + R+K+T PGA + K  EG+PN++NN++KGSL IT DV FPK+++  E ++ I + L    +  + YNGL GY
Sbjct:   11 LLLLYLIGAVIAGRDFYKILGVPRSASIKDIKKAYRKLALQLHPDRNPDDPQAQEKFQDLGAAYEVLSDSEKRKQYDTYGEEGLKDGHQS-SHGDIFSHFFGDFGFMFGGAPRQQDRNIPRGSDIIVDLEVTLEEVYAGNFVEVVRNKPVARQAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNVKLVNEERTLEVEIEPGVRDGMEYPFIGEGEPHVDGEPGDLRFRIKVVKHRIFERRGDDLYTNVTVSLVEALVGFEMDITHLDGHKVHISRDKITRPGAKLWKKGEGLPNFDNNNIKGSLIITFDVDFPKEQLTEEAKEGIKQLLKQGPVQKV-YNGLQGY 358          
BLAST of DnaJ-like protein vs. TrEMBL
Match: A0A1I8IXU4 (J domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig014669g1 PE=4 SV=1)

HSP 1 Score: 470.315 bits (1209), Expect = 9.726e-162
Identity = 243/336 (72.32%), Postives = 280/336 (83.33%), Query Frame = 1
Query:  136 YCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVF-FDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGL 1140
            + GRDFYKIL V R+A  NQ+KKAYR LAKQLHPD+N+DDP AE +F+DLNAAYE LSDPEKRKI+DA GEEGLKD  G G S DPFSSFFGD+F F  GG  ++++IPRGGDVV+DL V+LEELYNGNFVE+VR K VKK A G RRCNCRHEM+T   GPGRF MTQE+VCDEC NV  VPEEKLLE+EIEPGMRDGQ+YPFVAEGEPHIDGEPGDL+FRI Q  HPRF+RRGDDLYTN++I+L+DAL GFSM+I+HLDGHKV+V REKVTWPGA ++K+ EGMPNYENN+VKGSL+IT DV FPK      E+KE +K+LL  D     YNGL
Sbjct:   21 FAGRDFYKILGVQRAATVNQVKKAYRGLAKQLHPDRNRDDPKAEERFRDLNAAYEVLSDPEKRKIYDAHGEEGLKDGVGRGGS-DPFSSFFGDMFQFHTGGGNQEREIPRGGDVVVDLDVTLEELYNGNFVEIVRNKPVKKKAPGKRRCNCRHEMRTRSLGPGRFQMTQEEVCDECENVTLVPEEKLLELEIEPGMRDGQIYPFVAEGEPHIDGEPGDLRFRIHQLKHPRFQRRGDDLYTNLSITLVDALNGFSMDIEHLDGHKVRVTREKVTWPGAMLRKANEGMPNYENNNVKGSLFITFDVEFPKGLTLPPEDKEAVKRLLKQDSAQRVYNGL 355          
BLAST of DnaJ-like protein vs. TrEMBL
Match: A0A1I8JFG2 (J domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006868g1 PE=4 SV=1)

HSP 1 Score: 470.315 bits (1209), Expect = 1.016e-161
Identity = 243/336 (72.32%), Postives = 280/336 (83.33%), Query Frame = 1
Query:  136 YCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVF-FDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGL 1140
            + GRDFYKIL V R+A  NQ+KKAYR LAKQLHPD+N+DDP AE +F+DLNAAYE LSDPEKRKI+DA GEEGLKD  G G S DPFSSFFGD+F F  GG  ++++IPRGGDVV+DL V+LEELYNGNFVE+VR K VKK A G RRCNCRHEM+T   GPGRF MTQE+VCDEC NV  VPEEKLLE+EIEPGMRDGQ+YPFVAEGEPHIDGEPGDL+FRI Q  HPRF+RRGDDLYTN++I+L+DAL GFSM+I+HLDGHKV+V REKVTWPGA ++K+ EGMPNYENN+VKGSL+IT DV FPK      E+KE +K+LL  D     YNGL
Sbjct:   21 FAGRDFYKILGVQRAATVNQVKKAYRGLAKQLHPDRNRDDPKAEERFRDLNAAYEVLSDPEKRKIYDAHGEEGLKDGVGRGGS-DPFSSFFGDMFQFHTGGGNQEREIPRGGDVVVDLDVTLEELYNGNFVEIVRNKPVKKKAPGKRRCNCRHEMRTRSLGPGRFQMTQEEVCDECENVTLVPEEKLLELEIEPGMRDGQIYPFVAEGEPHIDGEPGDLRFRIHQLKHPRFQRRGDDLYTNLSITLVDALNGFSMDIEHLDGHKVRVTREKVTWPGAMLRKANEGMPNYENNNVKGSLFITFDVEFPKGLTLPPEDKEAVKRLLKQDSAQRVYNGL 355          
BLAST of DnaJ-like protein vs. TrEMBL
Match: A0A267E345 (J domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000996g2 PE=4 SV=1)

HSP 1 Score: 468.389 bits (1204), Expect = 4.896e-161
Identity = 242/336 (72.02%), Postives = 280/336 (83.33%), Query Frame = 1
Query:  136 YCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVF-FDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGL 1140
            + GRDFYKIL V R+A  NQ+KKAYR LAKQLHPD+N+DDP AE +F+DLNAAYE LSDPEKRKI+DA GEEGLKD  G G S DPFSSFFGD+F F  GG  ++++IPRGGDVV++L V+LEELYNGNFVE+VR K VKK A G RRCNCRHEM+T   GPGRF MTQE+VCDEC NV  VPEEKLLE+EIEPGMRDGQ+YPFVAEGEPHIDGEPGDL+FRI Q  HPRF+RRGDDLYTN++I+L+DAL GFSM+I+HLDGHKV+V REKVTWPGA ++K+ EGMPNYENN+VKGSL+IT DV FPK      E+KE +K+LL  D     YNGL
Sbjct:   21 FAGRDFYKILGVQRAATVNQVKKAYRGLAKQLHPDRNRDDPKAEERFRDLNAAYEVLSDPEKRKIYDAHGEEGLKDGVGRGGS-DPFSSFFGDMFQFHTGGGNQEREIPRGGDVVVNLDVTLEELYNGNFVEIVRNKPVKKKAPGKRRCNCRHEMRTRSLGPGRFQMTQEEVCDECENVTLVPEEKLLELEIEPGMRDGQIYPFVAEGEPHIDGEPGDLRFRIHQLKHPRFQRRGDDLYTNLSITLVDALNGFSMDIEHLDGHKVRVTREKVTWPGAMLRKANEGMPNYENNNVKGSLFITFDVEFPKGLTLPPEDKEAVKRLLKQDSAQRVYNGL 355          
BLAST of DnaJ-like protein vs. TrEMBL
Match: A0A0B7BIZ6 (J domain-containing protein (Fragment) OS=Arion vulgaris OX=1028688 GN=ORF187496 PE=4 SV=1)

HSP 1 Score: 457.988 bits (1177), Expect = 7.709e-157
Identity = 238/358 (66.48%), Postives = 281/358 (78.49%), Query Frame = 1
Query:   79 FALMNFLIILLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQ-KDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGLGGY 1149
            F   NFL++ L  FA      GRDFYK+L V RSA+   IK+AYR LAK+LHPDKN+DDP AE +FQ+L AAYE LSD EKR+I+D  GEEGLK    G +  DPFSSFFG   F  GG R Q +++PRGGDV++DL VSLEELYNGNF+EV+RYK V KSA G R+CNCR EM T Q GPGRF+MTQ+QVCDECPN++FVPEEK+LE+EIEPGMRDGQ YPFVAEGEPH+DGEPGDL+FRIRQ+ H RFERRGDDLYTNVT+ L DAL GF M IKHLDGH V V+R+K+TWPGA ++K  EGMPNY+NN+VKGSLYIT D+ FPK  V SEE+K+ +K +L  +     YNGL GY
Sbjct:   13 FLTANFLLLFLHVFA------GRDFYKVLGVSRSADIYHIKRAYRKLAKELHPDKNRDDPEAEERFQNLGAAYEVLSDKEKRQIYDKHGEEGLKQ---GDTRGDPFSSFFGGDLFGFGGMREQDREVPRGGDVILDLDVSLEELYNGNFIEVMRYKPVAKSAKGKRKCNCRTEMVTQQLGPGRFSMTQQQVCDECPNIKFVPEEKVLEIEIEPGMRDGQEYPFVAEGEPHMDGEPGDLRFRIRQTKHARFERRGDDLYTNVTVPLADALTGFEMNIKHLDGHMVHVVRDKITWPGARMRKPNEGMPNYDNNNVKGSLYITFDIEFPKG-VLSEEDKKGIKDMLKQESKQTVYNGLQGY 360          
BLAST of DnaJ-like protein vs. TrEMBL
Match: T1IYX8 (J domain-containing protein OS=Strigamia maritima OX=126957 PE=4 SV=1)

HSP 1 Score: 444.506 bits (1142), Expect = 1.767e-151
Identity = 222/352 (63.07%), Postives = 263/352 (74.72%), Query Frame = 1
Query:   97 LIILLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDV-FFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGLGGY 1149
            LI  +L F V  +   RDFY IL + RSAN+NQ+KKAYR LAK LHPDKNKDDP+A+ +FQDL AAYE LSDPEKR+++D  GEE LK   G GS  DPF+SFFGD  FF  G   + ++IPRG D+VMD+ V+LEELY+GNFVEVVR K   K A GTR+CNCR EM T Q GPGRF M Q+ VCDECPNV+ V EEK+LEVEIE GM+DG +  FVAEGEPH+DGEPGDL F+IR   HPRFERRGDDLYTNVTISL DAL GF ME+ HLD HKVKV R+K+TWPG  ++K  EGMPNY+NN++ G LYIT DV FPK  +  ++   I   L  T ++ + YNGL GY
Sbjct:   11 LIAFVLFFLVTEVINSRDFYAILGITRSANTNQVKKAYRRLAKDLHPDKNKDDPDAQDKFQDLGAAYEVLSDPEKRELYDRCGEECLKKDAGFGSG-DPFASFFGDFGFFQTGNKEQNREIPRGADIVMDISVTLEELYSGNFVEVVRNKPTIKPAKGTRKCNCRQEMVTRQLGPGRFQMMQQNVCDECPNVKLVNEEKVLEVEIEAGMQDGAMNRFVAEGEPHVDGEPGDLHFKIRTLPHPRFERRGDDLYTNVTISLSDALTGFEMELDHLDKHKVKVNRDKITWPGMRMRKKGEGMPNYDNNNLFGILYITFDVEFPKKDLTEQDTIAIKTILDQTSINNV-YNGLRGY 360          
BLAST of DnaJ-like protein vs. Ensembl Cavefish
Match: dnajb11 (DnaJ heat shock protein family (Hsp40) member B11 [Source:NCBI gene;Acc:103041146])

HSP 1 Score: 399.053 bits (1024), Expect = 3.965e-137
Identity = 212/352 (60.23%), Postives = 263/352 (74.72%), Query Frame = 1
Query:  100 IILLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKD--IPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGLGGY 1149
            I  LL + V  +  GRDFYKIL V +SA+   IKKAYR LA QLHPD+N DDP+A+ +F DL AAYE LSD EKRK +DA GEEGLK+ H   S  D FSSFFGD  F  GG+R+Q+D  IPRG D+++DL V+LEE+Y+GNFVEVVR K + K A G R+CNCR EM+T Q GPGRF MTQE VCDECPN++ V EE+ LEVEIE G+RD   YPF+ EGEPHIDGEPGDL+FRI+   HP FERRGDDLYTNVTISL++AL GF M+I HLDGHKV + R+K+T PG+ + K  EG+PN++NN+++GSL IT DV FPK+++  +++  I + L       + YNGL GY
Sbjct:   11 ICCLLLYLVATVLAGRDFYKILGVSKSASIKDIKKAYRKLALQLHPDRNPDDPSAQDKFADLGAAYEVLSDEEKRKQYDAYGEEGLKEGHHS-SHGDIFSSFFGDFGFMFGGNRQQQDRNIPRGNDIILDLEVTLEEVYSGNFVEVVRNKPIAKEAPGKRKCNCRQEMRTTQLGPGRFQMTQEVVCDECPNIKLVNEERTLEVEIEQGVRDEMEYPFIGEGEPHIDGEPGDLRFRIKVLKHPLFERRGDDLYTNVTISLVEALVGFEMQITHLDGHKVHIARDKITKPGSRLWKKGEGLPNFDNNNIRGSLIITFDVDFPKEQLDDQQKDGIKQLLKQAPAQKV-YNGLQGY 360          
BLAST of DnaJ-like protein vs. Ensembl Cavefish
Match: DNAJA4 (DnaJ heat shock protein family (Hsp40) member A4 [Source:NCBI gene;Acc:103041445])

HSP 1 Score: 163.31 bits (412), Expect = 2.210e-45
Identity = 118/335 (35.22%), Postives = 175/335 (52.24%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDS-HGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSV---KKSAHGTRR------CNCRH---EMKTIQTGPGRFTMTQEQV---------------CDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKS------MEGMPNYENNHVKGSLYITIDVAFP 1053
            +Y IL V   A++++IKKAYR LA + HPDKN   PN   +F+ ++ AYE L+DP+KR ++D  GE+ +K+   GGG    P   F  ++FF  GG  R +   RG +VV  L V+LEE+YNG+  ++   K+V   K   +G ++       NC+    ++K  Q GPG     Q                  C  C   +   ++K+LEV I+ GM+DGQ   F  EG+   D EPGD+   + Q  HP F+R+ ++L   + I L++AL GF   I+ LD   + +     + PG  IK +       EGMP Y + + KG L I  +V FP
Sbjct:    7 YYDILGVSPKASADEIKKAYRKLALKYHPDKN---PNEGEKFKLISQAYEVLADPKKRDLYDQGGEQAIKEGGMGGGDFSSPMDIF--NMFFGGGGGGRGQRERRGKNVVHQLGVTLEEMYNGSTRKLGLQKNVVCEKCDGYGGKKGTLEKCSNCKGKGVQIKVQQIGPGMIQQIQSMCSECQGQGEKFSAKDRCKNCNGQKVERKKKILEVHIDKGMKDGQKITFHGEGDQEPDLEPGDVIIVLEQKEHPVFQRQEENLIMKMQIKLVEALCGFKKTIRTLDNRTLII----SSPPGQVIKPNDVKCIQNEGMPLYRDPYEKGLLIIQFEVEFP 332          
BLAST of DnaJ-like protein vs. Ensembl Cavefish
Match: dnaja2b (DnaJ heat shock protein family (Hsp40) member A2b [Source:ZFIN;Acc:ZDB-GENE-030131-2884])

HSP 1 Score: 163.31 bits (412), Expect = 3.504e-45
Identity = 119/335 (35.52%), Postives = 167/335 (49.85%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDA-RGEEGLKDSHGGGSSMDPFSSFFGDVFFD----MGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSA--------HGTRRCN-CRHEMKTI---QTGPGRF---------------TMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHK--VKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFP 1053
             Y IL V  +A  N++KKAYR LAK+ HPDKN   PNA  +F++++ AYE L++PEK+ ++D    +   +   GG    D FS  FG   F          R     RG D+V  L VSLE+LYNG   ++   K+V  SA           ++C  CR     I   Q  PG                  +++   C +C   + + E K+LEV ++ GM+ GQ   F  E +   + EPGD+   +++  H  F R G+DL+ + TI L++AL GF + + HLDG    +K    KV  PG+T     EGMP Y N   KG LYI  DV FP
Sbjct:    9 LYDILGVSPTATENELKKAYRKLAKEYHPDKN---PNAGDKFKEISFAYEVLTNPEKKDLYDRYGEQGLREGGGGGAGMEDIFSHIFGGGLFGFMGGQSSRSRNGGRKRGEDMVHPLKVSLEDLYNGKTTKLQLSKNVLCSACNGQGGKTGAVQKCTTCRGRGMRIMIRQLAPGMVQQMQTVCSDCSGEGEVISERDRCKQCEGRKVIKEMKVLEVHVDKGMKHGQKITFSGEADQSPNTEPGDIILVLQEKEHEVFRRDGNDLHMSQTIGLVEALCGFQLTLTHLDGRHLLIKYPAGKVIEPGSTRVVRGEGMPQYRNPFEKGDLYIKFDVRFP 340          
BLAST of DnaJ-like protein vs. Ensembl Cavefish
Match: dnaja2a (DnaJ heat shock protein family (Hsp40) member A2 [Source:NCBI gene;Acc:103044841])

HSP 1 Score: 155.221 bits (391), Expect = 3.110e-42
Identity = 119/340 (35.00%), Postives = 172/340 (50.59%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGG----DVVMDLFVSLEELYNGNFVEVVRYKSVKKS--------AHGTRRCN-CRHEMKTI---QTGPGRF---------------TMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHK--VKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYS 1071
             Y IL V  SA+ N++KKAYR LAK+ HPDKN   PNA  +F++++ AYE LS+PEK++++D  GE+GL++  GGG  MD   S             + +    G     D+V  L VSLE++YNG   ++   K+V  S        +   ++C  CR     I   Q  PG                  ++++  C +C   + V E K+LEV ++ GM+ GQ   F  E +     EPGD+   +++  H  ++R G DL+    I L++AL GF   +KHLDG +  VK    KV  PG+      EGMP Y N   KG L+I  DV FP +   S
Sbjct:    9 LYDILGVSPSASENELKKAYRKLAKEYHPDKN---PNAGDKFKEISFAYEVLSNPEKKELYDRYGEQGLREGGGGGPGMDDIFSHIFGGGLFGFMGGQSRSRNGGRRRGEDMVHPLKVSLEDVYNGKTTKLQLSKNVLCSTCNGQGGKSGAVQKCTTCRGRGMRIMIRQLAPGMVQQMQSVCTDCNGEGEVISEKDRCKKCEGKKVVKEVKILEVHVDKGMKHGQKITFGGEADQAPGTEPGDIVLVLQEKEHETYKRDGHDLHMTHKIGLVEALCGFQFTLKHLDGRQIVVKYPAGKVIEPGSVKVVRGEGMPQYRNPFEKGDLFIKFDVQFPDNNWIS 345          
BLAST of DnaJ-like protein vs. Ensembl Cavefish
Match: dnaja1 (DnaJ heat shock protein family (Hsp40) member A1 [Source:ZFIN;Acc:ZDB-GENE-030131-2642])

HSP 1 Score: 150.214 bits (378), Expect = 1.856e-40
Identity = 114/335 (34.03%), Postives = 172/335 (51.34%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSS------MDPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSV--KKSAHGTRRC-NCRH---EMKTIQTGPGRFT---------------MTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATI----KKSM--EGMPNYENNHVKGSLYITIDVAFPK 1056
            FY +L V  +A+ +++KKAYR LA + HPDKN   P    +F+ ++ AYE LSD +KR+++D  GE+ +K+   G         +D F+ FFG      GGSR Q++  +  +VV  L VSLEELYNG   ++   K+V  +K       C +CR    +++  Q  PG                  ++    C  C   + + ++K+LEV I+ GM+D Q   F  EG+     EPGD+   + Q  HP F R  +DL   + + L+++L GF   +  LD ++V +IR   + PG  I    KK +  EGMP Y     KG L I  +V FP+
Sbjct:    7 FYDLLGVKPNASPDELKKAYRKLALKYHPDKN---PTEGEKFKQISQAYEVLSDAKKREVYDRGGEKAIKEGGNGSGGGGFGSPLDIFNMFFG------GGSRMQRE-RKVKNVVHQLTVSLEELYNGATKKLAVQKNVICEKCEGAMEVCPSCRGTGVQVRLHQLVPGMVQQISTICGGCHGQGQRLSHRDRCKACAGRKILRQKKILEVHIDKGMKDEQKIVFHGEGDQEPGLEPGDIIIVLNQREHPVFTRHDEDLIMTMDLQLVESLCGFEKPVNALD-NRVLLIR---SHPGELIRPGDKKCVLNEGMPIYRRPFEKGRLIIHFNVVFPE 327          
BLAST of DnaJ-like protein vs. Ensembl Sea Lamprey
Match: dnajb11 (DnaJ heat shock protein family (Hsp40) member B11 [Source:ZFIN;Acc:ZDB-GENE-031113-9])

HSP 1 Score: 333.954 bits (855), Expect = 3.403e-113
Identity = 177/293 (60.41%), Postives = 215/293 (73.38%), Query Frame = 1
Query:  298 EALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFS--SFFGDVFFDMGGSRRQ-KDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAE------GEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGLGGY 1149
            + LS+ EKRK +D  GEEGLK+  G GS  D FS         F  GGSR+Q ++IPRG DVVM+L V+LEE+Y+GNFVEVVR K V K   G RRCNCR EM+T Q GPGRF MTQE VCDECPNV+ V EE+ LEVEIEPG+RDG  YPF+ E      GEPHIDGEPGDL+FRI+   HP FERRGDDLYTN+TISL+DAL GF M+I HLDGHKVK++R+KVTWPGA I+K  EG+PN++NNH +GSL IT DV FPK ++  +++ ++ + L       + YNGL GY
Sbjct:    1 QVLSNEEKRKTYDRHGEEGLKEG-GHGSHADAFSHFFGDFGFEFFGGGSRQQDRNIPRGSDVVMELEVTLEEVYSGNFVEVVRNKPVAKQTSGKRRCNCRQEMRTTQLGPGRFQMTQETVCDECPNVKLVNEERTLEVEIEPGVRDGMEYPFLGEDPFTTLGEPHIDGEPGDLRFRIKVLKHPVFERRGDDLYTNITISLVDALVGFEMDIIHLDGHKVKIVRDKVTWPGARIRKKEEGLPNFDNNHHRGSLIITFDVDFPKGELTIQQKDDLKELLKQESAQNV-YNGLQGY 291          
BLAST of DnaJ-like protein vs. Ensembl Sea Lamprey
Match: DNAJA4 (DnaJ heat shock protein family (Hsp40) member A4 [Source:HGNC Symbol;Acc:HGNC:14885])

HSP 1 Score: 158.688 bits (400), Expect = 3.069e-44
Identity = 120/334 (35.93%), Postives = 162/334 (48.50%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSV---KKSAHGTR-----RC-NCRHEMKTIQTGPGRFTMTQE--QVCDEC--------PNVR--------FVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKS------MEGMPNYENNHVKGSLYITIDVAFP 1053
            +Y  L V  +A   ++KKAYR LA + HPDKN   PN   +F+ ++ AYE L DP+KR+++D  GE+ LK       +         D+FF+ GG+R  ++  RG + V  L V LEE+YNG+  ++   K+V   K    G R     RC  CR     +Q       M Q+   VC EC        P  R         V E+K+LEV I  GM+DGQ   F  EG+   D EPG +   + Q  HP F RR DDL   + I L++AL GF   IK LD   + +     +  G  IK+        EGMP Y +   KG L I   V FP
Sbjct:    7 YYDTLGVNPNATHEELKKAYRKLALKYHPDKN---PNEGEKFKQISQAYEVLLDPKKRELYDMGGEQALKGGSSNSGNGFSSPMDIFDLFFN-GGTRMHRE-RRGKNAVHQLSVGLEEMYNGSTRKLALQKNVLCAKCEGRGGRKGAVERCVPCRGTGMYLQIQQIGLGMVQQIQSVCPECHGHGERINPRHRCRYCNGRKIVREKKILEVHISKGMKDGQKITFYGEGDQDPDLEPGHIIIVLDQKEHPVFHRREDDLVMKMEIELVEALCGFQRSIKTLDNRTILI----TSHQGEVIKEGDVKCILNEGMPLYRDPFEKGRLIIQFSVKFP 331          
BLAST of DnaJ-like protein vs. Ensembl Sea Lamprey
Match: dnaja1 (DnaJ heat shock protein family (Hsp40) member A1 [Source:ZFIN;Acc:ZDB-GENE-030131-2642])

HSP 1 Score: 127.487 bits (319), Expect = 2.353e-33
Identity = 111/351 (31.62%), Postives = 160/351 (45.58%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSS-----MDPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNG--------------------NFVE------------VVRYKSVKKSAHG-------TRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEE---KLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSM------EGMPNYENNHVKGSLYITIDV 1044
            +Y +L V  ++ ++++KKAYR LA + HPDKN   PN   +F+ ++ AYE LSD +KR+++D  GE+ +K+   GGS      MD F+ FFG      GG   Q++  +G +VV  + VSLE+LYNG                    NF+             V+   SV+    G          C C  ++KT+         + +Q    C   +F+P      LL +    GM D Q   F  EG+     +PGD+   + Q  H  F R+G DL   + ISL +AL GF   IK LD   + +     + PG  IK         EGMP + N   KG L I   V
Sbjct:    7 YYDLLGVKPNSTADELKKAYRKLALKYHPDKN---PNEGEKFKQISQAYEVLSDAKKRELYDRGGEQAIKEGGAGGSGGFSSPMDIFNMFFG------GGGSMQRET-KGKNVVHQISVSLEDLYNGATRKLSMQKNVICDKCEGESNFLVTKPFNQNWCPRGVMTGYSVRCCVFGGGVFVFCAGHCFCLSQVKTV---------SPKQHVGWCCQ-QFIPASVKIYLLAINSAKGMTDNQKISFYGEGDQEPGLDPGDVIIVLDQKEHDVFRRKGQDLLMEMEISLTEALCGFRRPIKTLDNRTIVI----TSHPGEVIKYDAIKCVLNEGMPFHRNPFEKGRLIIHFKV 333          
BLAST of DnaJ-like protein vs. Ensembl Sea Lamprey
Match: dnaja2a (DnaJ heat shock protein family (Hsp40) member A2a [Source:ZFIN;Acc:ZDB-GENE-040426-2884])

HSP 1 Score: 119.783 bits (299), Expect = 5.238e-30
Identity = 111/344 (32.27%), Postives = 152/344 (44.19%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGG--------DVVMDLFVSLEELYNGNFVEVVRYKSVK------KSAHGTRRCNCRHEMKTIQTGPG---RFTMTQE---QVCDECPN-----------------VRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPR----FERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIRE--KVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFP 1053
             Y++L V  +AN   +KKAYR LAK+ HPDKN   P+A  +F++++ AYE LS+PEKR ++D  GE+GL++  GG    D FS  FG   F              G        D++  L VSLE+LY   +  V   K+        +     R C C H      +  G       +QE    +C+EC N                 VRF+    L +  +  GM    +  F  EG      E   L      + H R    F+R G+DL+    I L +AL G    + HLDG ++ V      V  PG       EGMP+Y N   KG+LYI  DV FP
Sbjct:    9 LYELLGVSPTANEGDLKKAYRKLAKEYHPDKN---PDAGDKFKEISFAYEVLSNPEKRDLYDRHGEKGLREGGGGPGMDDIFSHIFGGGLFGGFMGGGGGRGGHPGAGRRRRGEDMMHPLKVSLEDLYRATWALVSCAKNTAVEVCLWRVGASGRICLC-HNCSEANSRAGISHAVPSSQEWLAALCNECRNRSAPCPCRASPRTAERRVRFI---HLQKRAVHAGMAHTHMIRFKGEGPRGPCNERIFLLVLTTTTMHCRHTKVFQRDGNDLHMMKKIGLAEALCGVRFTLAHLDGRQILVTYPPGNVIQPGCIRGIVGEGMPHYRNPFEKGNLYIKFDVEFP 345          
BLAST of DnaJ-like protein vs. Ensembl Sea Lamprey
Match: dnaja3b (DnaJ heat shock protein family (Hsp40) member A3b [Source:ZFIN;Acc:ZDB-GENE-040115-3])

HSP 1 Score: 89.7373 bits (221), Expect = 9.077e-20
Identity = 84/295 (28.47%), Postives = 133/295 (45.08%), Query Frame = 1
Query:  145 RDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGL------------KDSHGGGSSMDP---FSSFFGDVF-FDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKS-------VKKSAHGTRRCNCRH----EMKTIQTGP-----------GRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPF-VAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHL 912
            +D+Y+IL V +      IKK+Y  LAK+ HPD NKDDP A+ +F +L  AYE LSD  KRK +D  G  G             +     GS++DP   F   FG+    + G      D P+  +  M+L  +  +   G   E+  + +        + +  GT+   C +     M+TI TGP           G+ T+     C  C       ++K + V +  G+ DGQ     V + E  I        FR+++S+   F R G D+++++ IS+  AL G +  ++ L
Sbjct:   12 KDYYQILGVAKDVAQKDIKKSYYQLAKKYHPDTNKDDPKAKEKFAELAEAYEVLSDDAKRKQYDTYGSAGFDAGFGSGAGTGGQQYWRAGSTVDPEELFRKIFGEFSRSNFGDFSSMFDQPQ--EFFMEL--TFTQAAKGVNKEIALFLNDTCQRCEGRGNEPGTKLQRCHYCNGTGMETINTGPFVMRSTCRRCSGKGTIMTNP-CTLCHGSGETKQKKTVVVPVPAGVEDGQTVRMPVGKKEIFIT-------FRVQKSA--TFRREGADVHSDLHISVAQALLGGTARVQGL 292          
BLAST of DnaJ-like protein vs. Ensembl Yeast
Match: YDJ1 (Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; acts as an adaptor that helps Rsp5p recognize cytosolic misfolded proteins for ubiquitination after heat shock; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family; chimeric protein in which human p58IPK J domain replaces yeast Ydj1p J domain can complement yeast ydj1 mutant [Source:SGD;Acc:S000005008])

HSP 1 Score: 158.688 bits (400), Expect = 1.981e-44
Identity = 121/347 (34.87%), Postives = 170/347 (48.99%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSM--------DPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSV-----------KKSAHGTRRCNCRH-EMKTIQTGP--GRF------------TMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGH--KVKVIREKVTWPGATIKKSMEG----MPNYENNHVKGSLYITIDVAFPKDKVYS 1071
            FY IL VP +A   +IKKAYR  A + HPDKN  +  AE +F++ +AAYE LSDPEKR I+D  GE+GL  + G G           D FS FFG      GG++R +   RG D+  ++  SLEELY G   ++   K +           K +      CN +  +  T Q GP   RF             +  +  C  C   +   E K+LEV +EPGM+DGQ   F  E +   D  PGD+ F + +  H  F+R GDDL     I LL A+ G    ++H+ G   KV ++  +V  PG  ++K +EG    +P Y      G+L I   + FP++   S
Sbjct:    7 FYDILGVPVTATDVEIKKAYRKCALKYHPDKNPSEEAAE-KFKEASAAYEILSDPEKRDIYDQFGEDGLSGAGGAGGFPGGGFGFGDDIFSQFFG-----AGGAQRPRGPQRGKDIKHEISASLEELYKGRTAKLALNKQILCKECEGRGGKKGAVKKCTSCNGQGIKFVTRQMGPMIQRFQTECDVCHGTGDIIDPKDRCKSCNGKKVENERKILEVHVEPGMKDGQRIVFKGEADQAPDVIPGDVVFIVSERPHKSFKRDGDDLVYEAEIDLLTAIAGGEFALEHVSGDWLKVGIVPGEVIAPG--MRKVIEGKGMPIPKYGGY---GNLIIKFTIKFPENHFTS 342          
BLAST of DnaJ-like protein vs. Ensembl Yeast
Match: XDJ1 (Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies [Source:SGD;Acc:S000004080])

HSP 1 Score: 107.842 bits (268), Expect = 5.888e-26
Identity = 97/378 (25.66%), Postives = 164/378 (43.39%), Query Frame = 1
Query:  142 GRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAES---QFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKDIP------------RGGDVVMDLFVSLEELYNGNFVEV-VRYKSVKKSAHGT-----RRCNCRHEMKTIQT------------GPGRFTMTQEQVCD--------------------ECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEP-GDLKFRIRQSSHPRFERR----------GDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREK---VTWPGATIKKSMEGMPNYENNHVK-GSLYITIDVAFPKDKVYS 1071
            G   Y +L V R A   +IK AYR LA + HPDK  D  + E    +F+++ AAYE LSDPEK+  +D  G++    S GG +             F   GS    + P               D+ +D+ ++L++LY G  ++  ++ + +    HG+     R+ +  H+++                GPG    +Q  VC+                    +C  +  + +++++ V + PG     +       +  ID    GDLKF + +      +++          G+DLYT++TISL +AL GF   +      ++  +  K   V  PG TIK + EG P  +N H + G LY+ + + FP D  ++
Sbjct:    7 GDRLYDVLGVTRDATVQEIKTAYRKLALKHHPDKYVDQDSKEVNEIKFKEITAAYEILSDPEKKSHYDLYGDDNGAASSGGANGFGDEDFMNFFNNFFNNGSHDGNNFPGEYDAYEEGNSTSSKDIDIDISLTLKDLYMGKKLKFDLKRQVICIKCHGSGWKPKRKIHVTHDVECESCAGKGSKERLKRFGPG-LVASQWVVCEKCNGKGKYTKRPKNPKNFCPDCAGLGLLSKKEIITVNVAPGHHFNDVITVKGMADEEIDKTTCGDLKFHLTEKQENLEQKQIFLKNFDDGAGEDLYTSITISLSEALTGFEKFLTKTFDDRLLTLSVKPGRVVRPGDTIKIANEGWPILDNPHGRCGDLYVFVHIEFPPDNWFN 383          
BLAST of DnaJ-like protein vs. Ensembl Yeast
Match: SCJ1 (One of several homologs of bacterial chaperone DnaJ; located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins [Source:SGD;Acc:S000004827])

HSP 1 Score: 104.375 bits (259), Expect = 4.233e-25
Identity = 102/346 (29.48%), Postives = 156/346 (45.09%), Query Frame = 1
Query:  142 GRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSM--------DPFSSF---FGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVE-VVRYKSVKKSAHGT-------RRC-NCRHE------------MKTIQTGPGRFTMTQEQV---CDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDL--KFRIRQSSHPRFERRGDDLYTNVTISLLDALQ-GFSMEIKHLDGHK-VKVIREK--VTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPK 1056
             +D+Y IL + + A   +IK AYR L+K+ HPDKN     A  +F ++  AY+ LSDPEK+KI+D  G + +K+  GGG           DPF  F   F       GG   Q+   RG  + +   +SL++ Y+G+ +E  +       + HG+        +C +C+               + IQ   GR   T + +   C  C   +   + K   V++ PG     +   V E E   D + GDL  +F+ + + +  + RRGD+LY    +S  +AL  G+   I+ LD +K VK+ R    V   G        GMP     +  G LYI   V  PK
Sbjct:   21 AQDYYAILEIDKDATEKEIKSAYRQLSKKYHPDKNAGSEEAHQKFIEVGEAYDVLSDPEKKKIYDQFGADAVKNGGGGGGPGGPGAGGFHDPFDIFERMFQGGHGGPGGGFGQRQRQRGPMIKVQEKLSLKQFYSGSSIEFTLNLNDECDACHGSGSADGKLAQCPDCQGRGVIIQVLRMGIMTQQIQQMCGRCGGTGQIIKNECKTCHGKKVTKKNKFFHVDVPPGAPRNYMDTRVGEAEKGPDFDAGDLVIEFKEKDTENMGYRRRGDNLYRTEVLSAAEALYGGWQRTIEFLDENKPVKLSRPAHVVVSNGEVEVVKGFGMPKGSKGY--GDLYIDYVVVMPK 364          
BLAST of DnaJ-like protein vs. Ensembl Yeast
Match: HLJ1 (Co-chaperone for Hsp40p; anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ [Source:SGD;Acc:S000004771])

HSP 1 Score: 70.8626 bits (172), Expect = 1.706e-14
Identity = 45/109 (41.28%), Postives = 59/109 (54.13%), Query Frame = 1
Query:  148 DFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEE---------GLKDSHGGGSSMDPFSSFFGDVFFD--MGGSR 441
            +FY+IL V R A  ++IKKAYR LA +LHPDKN   P A   F+ +N A+E LS+ EKR I+D  G +         G      G +   P    F D+FF+   GG R
Sbjct:   21 EFYEILKVDRKATDSEIKKAYRKLAIKLHPDKN-SHPKAGEAFKVINRAFEVLSNEEKRSIYDRIGRDPDDRQMPSRGAASGFRGSAGGSPMGGGFEDMFFNSRFGGQR 128          
BLAST of DnaJ-like protein vs. Ensembl Yeast
Match: CAJ1 (Nuclear type II J heat shock protein of the E. coli dnaJ family; contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly [Source:SGD;Acc:S000000850])

HSP 1 Score: 71.633 bits (174), Expect = 4.741e-14
Identity = 39/97 (40.21%), Postives = 57/97 (58.76%), Query Frame = 1
Query:  148 DFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFF-GDVFFDMGG 435
            ++Y IL +   A   +IKKAYR  A + HPDK+ DDP+A+++FQ +  AY+ LSDP  R  +D  G+E      G   + + F++ F GD F D  G
Sbjct:    6 EYYDILGIKPEATPTEIKKAYRRKAMETHPDKHPDDPDAQAKFQAVGEAYQVLSDPGLRSKYDQFGKEDAVPQQGFEDASEYFTAIFGGDGFKDWIG 102          
BLAST of DnaJ-like protein vs. Ensembl Nematostella
Match: EDO44765 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUW4])

HSP 1 Score: 397.512 bits (1020), Expect = 6.120e-137
Identity = 206/352 (58.52%), Postives = 260/352 (73.86%), Query Frame = 1
Query:   94 FLIILLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDM---GGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGL 1140
            F I LL+ F++  +  GRDFY IL VPR A+ NQIK+AYR LA +LHPDKNKDDP A+ +F D+ AAYE L+D ++RKI+D RGEEGLK++ G     DPFSSFFG   F      G    + +PRG D+ +DL V+LEELYNGNF+EV+R K   K+  GTR+CNCR EM+T+Q GPGRF M+ E++CDECP V +V +EK+LEVE+E GM+  Q YPFV+EGEPHIDGEPGDLKF+I +  H RF R+G DLY NVTI+L DAL GF M+I HLD HKV V+REK+TWPGA IKK  EGMP+YENNH +G LYIT DV FP+  + ++E+++I + L     H + YNGL
Sbjct:    8 FCISLLILFSIQTL-AGRDFYAILGVPRDASKNQIKRAYRKLAMKLHPDKNKDDPKAQEKFHDIGAAYEVLADDDQRKIYDQRGEEGLKNA-GHRDHSDPFSSFFGGFGFHFDGHNGHSHSQQVPRGSDLTVDLEVTLEELYNGNFIEVMRLKPETKTIPGTRKCNCRQEMRTVQLGPGRFQMSPEEICDECPAVTYVNKEKILEVEVEQGMKHEQEYPFVSEGEPHIDGEPGDLKFKIIELRHKRFTRKGKDLYANVTITLNDALSGFEMDIPHLDKHKVHVVREKITWPGARIKKKGEGMPSYENNHDRGDLYITFDVEFPRGSLDNQEKEDIQRILKQQSKHTI-YNGL 356          
BLAST of DnaJ-like protein vs. Ensembl Nematostella
Match: EDO43334 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZ26])

HSP 1 Score: 171.785 bits (434), Expect = 7.876e-49
Identity = 122/336 (36.31%), Postives = 169/336 (50.30%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTR--------RCNCRHE----MKTIQTGPGRFTMTQ----------EQV-----CDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIRE--KVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYS 1071
            +Y ILNVP +A + +IKK+YR LA + HPDKN   P+   +F+ ++ AYE LSD +KRKI+D  GE+ +K    GG    P   F  D+FF  G +  Q +  RG D+V  L V+LEELYNG   ++   K+V  S    R         C   H     ++  +  PG     Q          E++     C  C   + V E K+LEV I+ GM+DGQ   F  EG+     EPGD+   + +  HP F RRG DL   + I L+++L GF   I  LD   + V  +   V  PG       EGMP++ N   KG L I  DV FP++ V +
Sbjct:    7 YYDILNVPPTATATEIKKSYRKLALKYHPDKN---PDEGDRFKQISQAYEVLSDEKKRKIYDEGGEDAIKGGGEGGGFHSPMDIF--DMFFGTGRAAHQGE-RRGKDMVHQLRVTLEELYNGATRQLALQKNVICSKCDGRGGKEGCVESCQTCHGSGMYVRINRIAPGMVQQIQTVCRDCGGKGEKIPEKDRCKNCHGKKVVRERKILEVHIDKGMKDGQKRTFSGEGDQDPGIEPGDIVIVLDEQEHPVFRRRGADLIIQMEIDLVESLCGFQKTITTLDKRTLLVTSKPGNVIKPGDMKSIEDEGMPHHRNPFHKGRLLIQFDVKFPENGVLN 336          
BLAST of DnaJ-like protein vs. Ensembl Nematostella
Match: EDO36691 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHV1])

HSP 1 Score: 169.088 bits (427), Expect = 6.490e-48
Identity = 123/333 (36.94%), Postives = 168/333 (50.45%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGG--DVVMDLFVSLEELYNGNFVEVVRYKSV----KKSAHG-------TRRCNCRHEMKTIQ-TGPGRFTMTQEQV---------------CDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIR---EKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFP 1053
             Y +L VP++A+ N IKKAYR LAK+LHPDKN   P+   +F+D+  AYE LSDPEKR+++D  GE+GL++  GGG+  +   S          G        R    D+   L V+L +LYNG   ++   K+V     K A G          C  R    TI+  GPG     Q                  C +C   + V E K+LEV ++ GM DGQ   F  EG+   + EPGD+   I+Q  H  F R+G DL+   T++L +AL GF M +KHLDG  + +IR     +  PG       EGMP Y +   KG+LYI  D+ FP
Sbjct:    6 LYDLLGVPQNASDNDIKKAYRKLAKELHPDKN---PDTGEKFKDITFAYEILSDPEKRELYDRYGEKGLREGAGGGAGFEDILSHIFGGGSMPFGGGMGGRSRRRRGEDLFHPLKVTLADLYNGKTTKLQLSKNVICTTCKGAGGKPGAMRPCAGCKGRGVKVTIKPIGPGMVQQMQSMCHDCSGEGEVINPKDRCKKCQGKKVVKESKILEVHVDKGMSDGQKITFRGEGDQEPNVEPGDVILVIQQKDHDLFSRQGMDLFMTKTVTLAEALCGFHMVVKHLDGRDL-LIRYHAGNIIEPGCIRGIVGEGMPAYRHPFDKGNLYIKFDIEFP 334          
BLAST of DnaJ-like protein vs. Ensembl Nematostella
Match: EDO49360 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG53])

HSP 1 Score: 149.058 bits (375), Expect = 3.900e-41
Identity = 115/353 (32.58%), Postives = 167/353 (47.31%), Query Frame = 1
Query:  139 CGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDS----HGGGSSM------------------------------DPFSSFFGDV----FFDMGGS-----RRQK--DIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGD-DLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKD 1059
             G+D+Y +LNV ++A+++ IKKAYR  A + HPDKNK  P AE +F++++ AYE LSDP+K++I+D  GEEGLK +    +GGG                                 DPF+   G +    FF+  GS     R+QK  D P    +  DL VSLEELY G   ++   + V    +G++R                                   EEK+L V ++PG ++G    F  EG+      P D+ F+I+   H  F R GD +L     ISL DAL G ++ +  L G  V+V    V  PG++ +   EG+P  ++N  KG L I  DV  P +
Sbjct:    1 MGKDYYAVLNVDKAASADDIKKAYRKQALKYHPDKNK-SPGAEEKFKEISEAYEVLSDPKKKEIYDQYGEEGLKGTPPPQNGGGHGFSGANFGPGFTTFTYTSGDARETFSRVFGDEDPFADLIGGLGGFSFFNGMGSHQRKGRKQKVQDPP----LERDLLVSLEELYKGTTKKMKISRKV-PDPNGSQRL----------------------------------EEKILTVNVKPGWKEGTKITFPKEGDRKPGVIPADVVFKIKDKPHKHFTRDGDNNLVYKAKISLRDALGGTTISVPTLSGRTVQVHNADVIQPGSSKRIVGEGLPMPKDNSRKGDLIIKYDVYLPNN 313          
BLAST of DnaJ-like protein vs. Ensembl Nematostella
Match: EDO38685 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC61])

HSP 1 Score: 124.405 bits (311), Expect = 3.192e-32
Identity = 97/332 (29.22%), Postives = 150/332 (45.18%), Query Frame = 1
Query:  139 CGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDS-------------HGGGSSM--------DPFSSFFGDVFFDM------GGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFP 1053
             G D+Y IL + RSA    IKK YR L+ + HPDKN+ +P+AE +F+    AY+ LSDP+KR I++  GEEGLK               HG  + +        +PFS  F     D+      G  RR++D P    +  +L+++LEE++ G                      C  +MK       R  M ++       N+R    +K+L + ++ G R      F  EG+   +  P D+ F ++   HP F+R  D+L    T+ L  AL G  +++  LDG  + +    +  P        EGMP  +N   +G L I  ++ FP
Sbjct:    1 MGLDYYDILGLTRSATDADIKKEYRKLSLKYHPDKNQ-EPSAEVKFRQAAEAYDVLSDPKKRAIYNQFGEEGLKSGVPEKDAWTQGYTFHGDANRVFREFFGGNNPFSELFDSYDPDIGFGGIHGRGRRKQDPP----IERELYLTLEEVFKG----------------------CVKKMKI-----SRRVMNED---GHTSNIR----DKILTINVKRGWRASTKITFPKEGDQGPNNIPADIVFIVKDKPHPIFKRDNDNLIYIATVPLGKALTGCVVDVPTLDGRLISIPVNDIVKPEYQKVVPEEGMPISKNPDKRGDLIIQFNIEFP 293          
BLAST of DnaJ-like protein vs. Ensembl Medaka
Match: DNAJA4 (dnaJ homolog subfamily A member 4 [Source:NCBI gene;Acc:101166714])

HSP 1 Score: 178.718 bits (452), Expect = 3.355e-51
Identity = 122/336 (36.31%), Postives = 176/336 (52.38%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDS--HGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSV---KKSAHGTRR------CNCRH---EMKTIQTGPGRFTMTQEQV---------------CDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKS------MEGMPNYENNHVKGSLYITIDVAFP 1053
            FY +L V   A++++IKKAYR LA + HPDKN   PN   +F+ ++ AYE LSDP+KR ++D  GE+ +K+    GG S MD F+ FFG      GG R Q++  +G +VV  L VSLEE+YNG+  ++   K+V   K   +G ++       NC+    ++K  Q GPG     Q                  C  C   +   ++K+LEV I+ GM+DGQ   F  EG+     EPGD+   + Q  HP F+R+ +DL   + + L++AL GF   I+ LD   + +     T PG  IK +       EGMP Y + + KG L I   V FP
Sbjct:    7 FYDLLGVSPKASADEIKKAYRKLALKYHPDKN---PNEGEKFKLISQAYEVLSDPKKRDLYDHGGEQAIKEGGMSGGSSPMDIFNMFFG------GGGRMQRE-RKGKNVVHQLSVSLEEMYNGSTRKLGLQKNVICEKCDGYGGKKGALEKCANCKGRGVQVKVQQIGPGMIQQIQSMCPDCQGQGEKFNSKDRCKNCNGHKVERQKKILEVHIDKGMKDGQKITFHGEGDQEPGLEPGDVIIVLDQKDHPVFQRQDNDLVMRMNLKLVEALCGFRKTIQTLDNRTLII----STQPGEVIKHNDFKCIQNEGMPLYRDPYEKGQLIIQFQVEFP 328          
BLAST of DnaJ-like protein vs. Ensembl Medaka
Match: dnaja2a (DnaJ heat shock protein family (Hsp40) member A2 [Source:NCBI gene;Acc:101173536])

HSP 1 Score: 157.532 bits (397), Expect = 4.642e-43
Identity = 121/341 (35.48%), Postives = 173/341 (50.73%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGG-----DVVMDLFVSLEELYNGNFVEVVRYKSVKKSA--------HGTRRCN-CRHEMKTI---QTGPGRF---------------TMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHK--VKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYS 1071
             Y IL V  SA  N++KKAYR LAK+ HPDKN   PNA  +F++++ AYE LS+PEK++++D  GE+GL++  GGG  MD   S             +      GG     D+V  L VSLE+LYNG   ++   K+V  S+           ++C+ CR     I   Q  PG                  ++++  C +C   + V E K+LEV ++ GM+ GQ   F  E +     EPGD+   +++  H  F+R  +DL+ N  I L++AL GF   +KHLDG +  +K    KV  PG+      EGMP Y N   KG LY+  DV FP +   S
Sbjct:    9 LYDILGVSPSATENELKKAYRKLAKEYHPDKN---PNAGDKFKEISFAYEVLSNPEKKELYDRYGEQGLREGGGGGPGMDDIFSHIFGGGLFGFMGGQGNRSRNGGRRRGEDMVHPLKVSLEDLYNGKTTKLQLSKNVLCSSCNGQGGKTGAVQKCSTCRGRGMRIMIRQLAPGMVQQMQSVCTDCNGEGEVISEKDRCKKCEGKKVVKEVKILEVHVDKGMKHGQKITFGGEADQAPGVEPGDIVLVLQEKEHETFKREKNDLFINHKIGLVEALCGFQFTLKHLDGRQIVIKYPAGKVIEPGSVRVVRGEGMPQYRNPFEKGDLYVKFDVQFPDNNWIS 346          
BLAST of DnaJ-like protein vs. Ensembl Medaka
Match: dnaja1 (dnaJ homolog subfamily A member 1 [Source:NCBI gene;Acc:101162427])

HSP 1 Score: 155.992 bits (393), Expect = 1.659e-42
Identity = 116/342 (33.92%), Postives = 167/342 (48.83%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGG--------GSSMDPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSV---KKSAHGTRR-----CNCRH----EMKTIQTGPGRFT---------------MTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKS------MEGMPNYENNHVKGSLYITIDVAFP 1053
            FY  L V  +A  +++KKAYR LA + HPDKN   P    +F+ ++ AYE LSDP+KR+I+D  GE+ LK+   G         S MD F  FFG      GGSR Q++  +G ++V  + VSLEELYNG   ++   K+    +    G+R+     C   H    +++  Q  PG                  ++ +  C  C   + + ++K+LEV I+ GM+DGQ   F  EG+     EPGD+   +    H  F RRG DL  ++ + L++AL GF   ++ LD   + V     + PG  IK         EGMP Y     KG L I   V FP
Sbjct:    7 FYDTLGVQANATLDELKKAYRKLALKYHPDKN---PTEGEKFKQISQAYEVLSDPQKREIYDRGGEKALKEGGTGGGGGGGGFASPMDIFDFFFG------GGSRMQRE-RKGKNMVHQITVSLEELYNGATRKLAVQKNCICERCEGRGSRKGAAQVCMSCHGTGMQVRVHQLLPGMVQQVSTVCSSCQGQGQRISHKDRCKACGGRKILRQKKILEVHIDKGMKDGQKIVFHGEGDQEPGLEPGDIIIVLDLREHSLFTRRGSDLVMSMELQLVEALCGFKRPVQTLDNRTLFV----TSHPGELIKPEDTKCILNEGMPIYRRPFEKGLLVIHFSVVFP 334          
BLAST of DnaJ-like protein vs. Ensembl Medaka
Match: dnaja2b (dnaJ homolog subfamily A member 2-like [Source:NCBI gene;Acc:101175395])

HSP 1 Score: 149.828 bits (377), Expect = 2.875e-40
Identity = 117/340 (34.41%), Postives = 163/340 (47.94%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDA-RGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGG---DVVMDLFVSLEELYNGNFVEVVRYKSVKKSAH--------GTRRC-NCRHEMKTI---QTGPGRFTMTQEQV---------------CDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHK--VKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYS 1071
             Y IL V  SA+ N++KKAYR LAK+ HPDKN   P A  +F++++ AYE L++PEK++++D    +   +   GG    D FS  FG   F   G + +          D++  L VSLE+LYNG   ++   K+V  SA           ++C  CR     I   Q  PG     Q                  C +C   +   + KLLEV ++ GM+ GQ   F  E +     EPGD+   +++  H  F R G DL+    I L++AL GF M + HLDG +  +K    KV  PG       EGMP Y N   KG LYI  DV FP++   S
Sbjct:    9 LYDILGVSPSASENELKKAYRKLAKEYHPDKN---PEAGDKFKEISFAYEVLTNPEKKELYDRYGEQGLREGGGGGPGMEDIFSHIFGGGLFGFMGGQSRGRNGGKRRGDDMIHPLKVSLEDLYNGKTTKLQLSKNVLCSACNGQGGKAGAVQKCVACRGRGMRIMIRQLAPGMVQQMQSVCTDCSGEGEVINEKDRCKKCEGQKVCKDTKLLEVHVDKGMKHGQKITFSGEADQAPGVEPGDIVLVLQEKEHEDFRREGSDLHMVHRIGLVEALCGFQMTVTHLDGRQLLIKYPPGKVIEPGCIRVVKGEGMPQYRNPFEKGDLYIKFDVQFPENNWIS 345          
BLAST of DnaJ-like protein vs. Ensembl Medaka
Match: zgc:122979 (dnaJ homolog subfamily B member 5 [Source:NCBI gene;Acc:101168635])

HSP 1 Score: 138.272 bits (347), Expect = 3.326e-36
Identity = 104/346 (30.06%), Postives = 160/346 (46.24%), Query Frame = 1
Query:  142 GRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSS-------MDP---FSSFFG-----DVFF--------------------DMG-------GSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFP 1053
            G+DFYKIL +   +N ++IKKAYR LA + HPDKN  DP+AE +F+++  AYE L+DP+KR ++D  GEEGLK+    G          DP   FSSFF      D+FF                    ++G       G R+ +    G   V DL V+LE++ +G                      C   +K          +T+ ++  +  ++R   EEK+L V ++ G + G    F  EG+      P D+ F +R   HP + R G ++     I+L +AL G ++ +  LD   + +    V  PGA  +   EG+P  ++   +G L +   V FP
Sbjct:   54 GKDFYKILGITHESNEDEIKKAYRKLALKFHPDKNS-DPDAEDKFKEIAEAYEVLTDPQKRSVYDQFGEEGLKNGVSTGQGGFRNNFPTDPHATFSSFFHGSDHFDIFFGSDPESDDDLFNPFRRFTFTNLGGFAGYEAGQRKGQQWLPGQAAVHDLLVTLEDVMHG----------------------CTKHVK----------VTRSRLNPDGRSLR--SEEKVLNVVVKKGWKAGTKITFPREGDETPGSGPADITFILRDEEHPTYRRDGSNIVYTAQITLKEALCGCTVNVPTLDSRMMPLPCSDVIKPGAVRRLRGEGLPLPKSPSQRGDLMVEFQVLFP 364          
BLAST of DnaJ-like protein vs. Planmine SMEST
Match: SMESG000077692.1 (SMESG000077692.1)

HSP 1 Score: 722.235 bits (1863), Expect = 0.000e+0
Identity = 359/359 (100.00%), Postives = 359/359 (100.00%), Query Frame = 1
Query:   88 MNFLIILLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSXXXXXXXXXXXSTDMHPLEYNGLGGYKSGSL 1164
            MNFLIILLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSEEEKEILKKLLSTDMHPLEYNGLGGYKSGSL
Sbjct:    1 MNFLIILLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPKDKVYSEEEKEILKKLLSTDMHPLEYNGLGGYKSGSL 359          
BLAST of DnaJ-like protein vs. Planmine SMEST
Match: SMESG000012107.1 (SMESG000012107.1)

HSP 1 Score: 159.073 bits (401), Expect = 1.055e-43
Identity = 116/337 (34.42%), Postives = 169/337 (50.15%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSMDPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSV---KKSAHG-----TRRCN-CRH---EMKTIQTGPG---------------RFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKK------SMEGMPNYENNHVKGSLYITIDVAFPKDK 1062
            +Y IL V  + +  ++KKAYR LA + HPDKN   P+A  +F++++ A+E L+D +KR+I+D  GE+ LK+  G     +P   F  D+FF   G  R +   +G DV+  L V+L+ELY GN  ++   K+V   K S  G      ++C  CR    E+   Q GPG               R  + +   C  C   + V E+K+LEV I+ GM DGQ   F  EG+     EPGD+   + +  H  F R+  DL   + + L+D+L GF   I  LD   + V     T PG  IK         EGMP ++N    G L I  +V FP+D+
Sbjct:    7 YYDILGVHPNVSEQELKKAYRKLALKYHPDKN---PDAGDKFKEISQAFEVLADAKKRQIYDEGGEQALKEGGGDSGFHNPMDIF--DMFFGGMGGGRSRGPRKGKDVIHQLNVTLDELYKGNTRKLAIQKNVICDKCSGRGGKEGAVQKCTTCRGMGVEVHIRQLGPGMVQQMQTTCRTCKGEREVINERDRCKRCEGQKVVREKKVLEVHIDKGMSDGQQIKFSGEGDQEPGLEPGDICIVLEEKPHNVFTRKKADLIYTMKLDLVDSLCGFKRTITTLDSRTLVV----ETKPGEVIKNLEYRALENEGMPKHKNPFEHGKLIIQFEVVFPEDQ 334          
BLAST of DnaJ-like protein vs. Planmine SMEST
Match: SMESG000004726.1 (SMESG000004726.1)

HSP 1 Score: 145.206 bits (365), Expect = 1.339e-38
Identity = 114/342 (33.33%), Postives = 158/342 (46.20%), Query Frame = 1
Query:  151 FYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGG--SSMDPFSSFFGDVFFDMGGS-----RRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSV---KKSAHGTRR------CNCRH---EMKTIQTGPGRFTMTQEQVCDECPNV----------------RFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKS------MEGMPNYENNHVKGSLYITIDVAFP 1053
            +Y IL V  +A    +KKAYR LA + HPDKN   P+   +F++++ A+E LSD +KRKI+D  GEE LK+  GG   + MD          FDM         RQ+   RG D +  + V+LEELYNG    +   K +   K    G +         C+    E+   Q GPG     Q   C +C                   + V   K  ++ IE GM DGQ      EG+     E GDL   +++  H  ++R  +DL T V + L+D+L GF   +K LD   + V     + PG  IK +       EGMP Y++   KGSL I  DV FP
Sbjct:    7 YYDILEVKPNATEQDLKKAYRKLALKYHPDKN---PSGGEKFKEISQAFEVLSDSKKRKIYDEAGEEALKEGAGGNFHNPMD---------IFDMFFGGFGGLNRQRGPKRGKDNIKPMPVTLEELYNGATKNLAVSKKIICDKCEGRGGKEGAVESCATCKGKGIEIHIRQIGPGMVQQMQ-TTCSKCQGKGETIKDKDKCKKCSGNKVVHVTKNYDIHIEKGMMDGQSIRMSGEGDQESGVETGDLVVLLQEKPHEVYKRMKNDLLTTVKLDLVDSLCGFKRVMKTLDNRHIIV----QSSPGEIIKTNDFRKIESEGMPRYKSPFEKGSLIINFDVVFP 331          
BLAST of DnaJ-like protein vs. Planmine SMEST
Match: SMESG000076708.1 (SMESG000076708.1)

HSP 1 Score: 135.961 bits (341), Expect = 4.284e-36
Identity = 99/334 (29.64%), Postives = 152/334 (45.51%), Query Frame = 1
Query:  142 GRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGS-------------------------SMDPFSSFF----GDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFPK 1056
            G+D+Y IL + R+A    IKKAYR  A +LHP KN  DP+A  +F  ++ AYE LSDP KR ++D  GEEGLK+    GS                         + +PF  F+    GD+    GG   +  + +   +  DL ++LEE+Y+G          +KK     R  N      +I+                         +K+L V ++ G ++G    F  EG+   +  P D+ F ++  +H RF R   +L  + +ISL DAL G +++I  LD   + +    + +PG       EGMP+     +KG L+I   + FPK
Sbjct:    2 GQDYYSILCIQRNAKDGDIKKAYRMFALKLHPLKNISDPSAFEKFSLISEAYEVLSDPSKRAVYDQFGEEGLKNGIPHGSIEAGAWTKGYTYHGNPDKTFINFFGADNPFQEFYDRMDGDMHLSFGGLYGRSQMLQDPSIERDLNLTLEEIYHG---------CIKKLKISRRVMNEDGHTSSIR-------------------------DKILSVNVQCGWKEGTKITFPKEGDQGPNIIPADVVFILKDRAHSRFRRDEANLIYSSSISLADALIGCTIDIVTLDDRLLHIPITDIIYPGYVKIVPGEGMPHPSKPDMKGDLHIQFKIEFPK 301          
BLAST of DnaJ-like protein vs. Planmine SMEST
Match: SMESG000073794.1 (SMESG000073794.1)

HSP 1 Score: 119.783 bits (299), Expect = 6.395e-30
Identity = 99/330 (30.00%), Postives = 169/330 (51.21%), Query Frame = 1
Query:  142 GRDFYKILNVPRSANSNQIKKAYRNLAKQLHPDKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGG----------------GSSMDPFSSFFG--DVFFDMGGSRRQKDIPRGGDVVMDL---FVSLEELYNGNFVEVVRYKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNV---RFVPEEKLLEVEIEPGMRDGQLYPFVAEGEPHI-DGEPGDLKFRIRQSSHPRFERRGDDLYTNVTISLLDALQGF-SMEIKHLDGHKVKVIREKVTWPGATIKKSMEGMPNYENNHVKGSLYITIDVAFP 1053
            G+D+Y IL   + A+ ++IKKAYR +A + HPDKNK  PNAE++F+++  AYE LSDP+K++I+D  GEEGLK   GG                G   + F +FFG  D F ++ G+  ++   RG D  MD+   F     L++G       +   +         +    ++ + +G    T+ + ++  + P+      + EEK+LE++++ G + G    F  EG+ ++ D  P D+ F I+  +H  F+R G D+   V ISL DAL G  +++I  L+   + +    V  P    +    G+P+ +N   +G + +  ++ FP
Sbjct:    2 GKDYYSILGTQKGASDDEIKKAYRKMALKYHPDKNKS-PNAEAKFKEIAEAYEVLSDPKKKEIYDKYGEEGLKGGMGGGSDGSGGPGGFTYTFHGDPREIFKNFFGTDDPFANIFGNHSRR---RGADNFMDVDDDFHPGISLFSGFNTGGGSFPHAQMKQDPPIYHDLSVSLEDVLSG----TLKKIKITRKRPSPAGNSLIDEEKILEIDVKKGWKAGTKITFPREGDKYLTDNIPADIVFVIKDRTHRHFKRDGCDIRIKVKISLKDALCGIPNLQIPTLENQMINLPINTVIKPDTQKRIPGRGLPDNKNPSRRGDIVVEFEIIFP 323          
The following BLAST results are available for this feature:
BLAST of DnaJ-like protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
DNAJB117.099e-14362.00DnaJ heat shock protein family (Hsp40) member B11 ... [more]
DNAJB117.099e-14362.00DnaJ heat shock protein family (Hsp40) member B11 ... [more]
AC068631.32.112e-5765.65novel protein[more]
DNAJA22.355e-4936.12DnaJ heat shock protein family (Hsp40) member A2 [... [more]
DNAJA11.387e-4835.57DnaJ heat shock protein family (Hsp40) member A1 [... [more]
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BLAST of DnaJ-like protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dnj-206.482e-13458.92DnaJ homolog dnj-20 [Source:UniProtKB/Swiss-Prot;... [more]
dnj-131.062e-3931.53DNaJ domain (Prokaryotic heat shock protein) [Sou... [more]
dnj-131.062e-3931.53DNaJ domain (Prokaryotic heat shock protein) [Sou... [more]
dnj-197.497e-3532.77DNaJ domain (Prokaryotic heat shock protein) [Sou... [more]
dnj-197.497e-3532.77DNaJ domain (Prokaryotic heat shock protein) [Sou... [more]
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BLAST of DnaJ-like protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
shv3.661e-14562.67gene:FBgn0031256 transcript:FBtr0078060[more]
Droj22.053e-3933.92gene:FBgn0038145 transcript:FBtr0082751[more]
Droj22.053e-3933.92gene:FBgn0038145 transcript:FBtr0082754[more]
Droj22.053e-3933.92gene:FBgn0038145 transcript:FBtr0082752[more]
Droj22.053e-3933.92gene:FBgn0038145 transcript:FBtr0082753[more]
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BLAST of DnaJ-like protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dnajb112.085e-13959.21DnaJ heat shock protein family (Hsp40) member B11 ... [more]
dnaja2a1.680e-4734.71DnaJ heat shock protein family (Hsp40) member A2a ... [more]
dnaja12.660e-4534.32DnaJ heat shock protein family (Hsp40) member A1 [... [more]
dnaja2b4.546e-4235.01DnaJ heat shock protein family (Hsp40) member A2b ... [more]
CABZ01080568.14.185e-4034.98pep chromosome:GRCz11:25:34662358:34670413:-1 gene... [more]
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BLAST of DnaJ-like protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dnajb111.156e-14059.66DnaJ heat shock protein family (Hsp40) member B11 ... [more]
dnajb116.077e-13962.65DnaJ heat shock protein family (Hsp40) member B11 ... [more]
dnajb116.074e-13865.16DnaJ heat shock protein family (Hsp40) member B11 ... [more]
dnajb111.300e-13763.41DnaJ heat shock protein family (Hsp40) member B11 ... [more]
dnajb113.591e-13765.05DnaJ heat shock protein family (Hsp40) member B11 ... [more]
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BLAST of DnaJ-like protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Dnajb119.974e-14261.71DnaJ heat shock protein family (Hsp40) member B11 ... [more]
Dnajb119.974e-14261.71DnaJ heat shock protein family (Hsp40) member B11 ... [more]
Dnajb119.974e-14261.71DnaJ heat shock protein family (Hsp40) member B11 ... [more]
Dnaja29.066e-4935.82DnaJ heat shock protein family (Hsp40) member A2 [... [more]
Dnaja12.478e-4835.28DnaJ heat shock protein family (Hsp40) member A1 [... [more]
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BLAST of DnaJ-like protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9VPQ2|DJSHV_DROME3.669e-14462.67DnaJ homolog shv OS=Drosophila melanogaster OX=722... [more]
sp|Q5RAJ6|DJB11_PONAB3.409e-14262.00DnaJ homolog subfamily B member 11 OS=Pongo abelii... [more]
sp|Q9UBS4|DJB11_HUMAN3.409e-14262.00DnaJ homolog subfamily B member 11 OS=Homo sapiens... [more]
sp|Q99KV1|DJB11_MOUSE6.977e-14161.71DnaJ homolog subfamily B member 11 OS=Mus musculus... [more]
sp|Q6TUG0|DJB11_RAT7.865e-14161.71DnaJ homolog subfamily B member 11 OS=Rattus norve... [more]
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BLAST of DnaJ-like protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8IXU49.726e-16272.32J domain-containing protein OS=Macrostomum lignano... [more]
A0A1I8JFG21.016e-16172.32J domain-containing protein OS=Macrostomum lignano... [more]
A0A267E3454.896e-16172.02J domain-containing protein OS=Macrostomum lignano... [more]
A0A0B7BIZ67.709e-15766.48J domain-containing protein (Fragment) OS=Arion vu... [more]
T1IYX81.767e-15163.07J domain-containing protein OS=Strigamia maritima ... [more]
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BLAST of DnaJ-like protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dnajb113.965e-13760.23DnaJ heat shock protein family (Hsp40) member B11 ... [more]
DNAJA42.210e-4535.22DnaJ heat shock protein family (Hsp40) member A4 [... [more]
dnaja2b3.504e-4535.52DnaJ heat shock protein family (Hsp40) member A2b ... [more]
dnaja2a3.110e-4235.00DnaJ heat shock protein family (Hsp40) member A2 [... [more]
dnaja11.856e-4034.03DnaJ heat shock protein family (Hsp40) member A1 [... [more]
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BLAST of DnaJ-like protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dnajb113.403e-11360.41DnaJ heat shock protein family (Hsp40) member B11 ... [more]
DNAJA43.069e-4435.93DnaJ heat shock protein family (Hsp40) member A4 [... [more]
dnaja12.353e-3331.62DnaJ heat shock protein family (Hsp40) member A1 [... [more]
dnaja2a5.238e-3032.27DnaJ heat shock protein family (Hsp40) member A2a ... [more]
dnaja3b9.077e-2028.47DnaJ heat shock protein family (Hsp40) member A3b ... [more]
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BLAST of DnaJ-like protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
YDJ11.981e-4434.87Type I HSP40 co-chaperone; involved in regulation ... [more]
XDJ15.888e-2625.66Chaperone with a role in facilitating mitochondria... [more]
SCJ14.233e-2529.48One of several homologs of bacterial chaperone Dna... [more]
HLJ11.706e-1441.28Co-chaperone for Hsp40p; anchored in the ER membra... [more]
CAJ14.741e-1440.21Nuclear type II J heat shock protein of the E. col... [more]
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BLAST of DnaJ-like protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO447656.120e-13758.52Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO433347.876e-4936.31Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO366916.490e-4836.94Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO493603.900e-4132.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO386853.192e-3229.22Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of DnaJ-like protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
DNAJA43.355e-5136.31dnaJ homolog subfamily A member 4 [Source:NCBI gen... [more]
dnaja2a4.642e-4335.48DnaJ heat shock protein family (Hsp40) member A2 [... [more]
dnaja11.659e-4233.92dnaJ homolog subfamily A member 1 [Source:NCBI gen... [more]
dnaja2b2.875e-4034.41dnaJ homolog subfamily A member 2-like [Source:NCB... [more]
zgc:1229793.326e-3630.06dnaJ homolog subfamily B member 5 [Source:NCBI gen... [more]
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BLAST of DnaJ-like protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000077692.10.000e+0100.00SMESG000077692.1[more]
SMESG000012107.11.055e-4334.42SMESG000012107.1[more]
SMESG000004726.11.339e-3833.33SMESG000004726.1[more]
SMESG000076708.14.284e-3629.64SMESG000076708.1[more]
SMESG000073794.16.395e-3030.00SMESG000073794.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027362 ID=SMED30027362|Name=DnaJ-like protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1383bp
TTTAAATTCTGATTGTTTTAACGGAATATTACTTTATGTCAAAGAACCAA
TAAAAATTTTCCAACGTACTTTAAGTGATTTGCATTAATGAATTTTTTAA
TTATTTTACTTTTGAGTTTTGCTGTTTATCCTATTTACTGTGGCCGAGAT
TTTTATAAAATTTTGAACGTTCCAAGGTCTGCTAATTCCAATCAAATAAA
GAAGGCATACAGAAATCTTGCAAAACAACTTCATCCAGATAAAAATAAAG
ATGATCCTAATGCTGAATCGCAATTTCAAGATTTAAATGCTGCTTATGAG
GCTTTATCTGATCCTGAAAAGCGAAAAATCTTTGATGCACGCGGAGAAGA
AGGTTTAAAAGATTCTCATGGAGGTGGTTCATCAATGGATCCATTTTCAA
GTTTTTTTGGTGATGTATTTTTTGATATGGGTGGAAGTCGCCGTCAAAAA
GATATACCCAGAGGTGGTGATGTTGTAATGGATTTGTTTGTAAGTTTAGA
AGAGCTGTATAATGGAAATTTTGTTGAAGTTGTTCGTTATAAATCTGTTA
AAAAATCTGCACACGGAACTCGAAGATGCAATTGTCGACATGAAATGAAA
ACCATTCAAACCGGCCCAGGTCGTTTCACAATGACTCAAGAACAAGTATG
CGACGAATGCCCAAATGTCAGGTTTGTTCCCGAAGAAAAGTTATTAGAAG
TTGAAATTGAACCGGGAATGCGTGATGGACAGCTTTATCCATTTGTTGCC
GAAGGTGAACCCCATATTGATGGCGAGCCGGGCGATCTGAAATTTAGAAT
AAGACAATCATCGCATCCAAGATTCGAAAGACGTGGTGATGATCTATATA
CAAATGTGACAATTAGTTTGTTAGACGCATTACAAGGATTTTCGATGGAG
ATTAAGCATCTAGATGGACACAAGGTAAAAGTAATTCGTGAAAAGGTGAC
TTGGCCAGGTGCCACAATTAAGAAGTCTATGGAAGGAATGCCAAATTATG
AAAACAATCATGTAAAAGGATCTTTATATATAACGATAGATGTTGCATTC
CCAAAAGACAAAGTATATTCAGAGGAAGAAAAGGAAATTCTTAAAAAGTT
GTTATCAACGGATATGCACCCACTGGAATATAATGGTTTAGGAGGATATA
AATCTGGTTCCCTTTGAGAAGAATGCATTTAAAATAATTATAAATTTTAT
ACCATTATCTCAATGGCAGAAAATCATAATTTTATTTGCTCTGTTGGTCT
TTCTAGTGTGAGGATGTAATACTGTTTTCAATATTATTTCTTTGTTGGTT
TATCTGTAGATACACCCAATTATTGATAAATCAAGGATTATTTTCCTTGA
TTTTTTGTAAATTTTAATAAATAATGTTAAAGG
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protein sequence of SMED30027362-orf-1

>SMED30027362-orf-1 ID=SMED30027362-orf-1|Name=SMED30027362-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=360bp
MNFLIILLLSFAVYPIYCGRDFYKILNVPRSANSNQIKKAYRNLAKQLHP
DKNKDDPNAESQFQDLNAAYEALSDPEKRKIFDARGEEGLKDSHGGGSSM
DPFSSFFGDVFFDMGGSRRQKDIPRGGDVVMDLFVSLEELYNGNFVEVVR
YKSVKKSAHGTRRCNCRHEMKTIQTGPGRFTMTQEQVCDECPNVRFVPEE
KLLEVEIEPGMRDGQLYPFVAEGEPHIDGEPGDLKFRIRQSSHPRFERRG
DDLYTNVTISLLDALQGFSMEIKHLDGHKVKVIREKVTWPGATIKKSMEG
MPNYENNHVKGSLYITIDVAFPKDKVYSEEEKEILKKLLSTDMHPLEYNG
LGGYKSGSL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0000418head
PLANA:0002056medial muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR036869J_dom_sf
IPR001623DnaJ_domain
IPR002939DnaJ_C
IPR018253DnaJ_domain_CS
IPR008971HSP40/DnaJ_pept-bd
Vocabulary: molecular function
TermDefinition
GO:0051082unfolded protein binding
GO:0005198structural molecule activity
Vocabulary: biological process
TermDefinition
GO:0006457protein folding
GO:0007155cell adhesion
Vocabulary: cellular component
TermDefinition
GO:0005578proteinaceous extracellular matrix
GO:0005581collagen trimer
GO:0031012extracellular matrix
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 78..97
score: 32.98
coord: 23..41
score: 48.03
coord: 41..56
score: 55.13
coord: 58..78
score: 46.36
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 20..78
e-value: 1.5E-28
score: 110.8
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 21..83
e-value: 5.4E-26
score: 90.5
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 21..86
score: 22.153
IPR001623DnaJ domainCDDcd06257DnaJcoord: 21..75
e-value: 2.35997E-25
score: 94.9197
IPR036869Chaperone J-domain superfamilyGENE3DG3DSA:1.10.287.110coord: 19..119
e-value: 3.5E-30
score: 106.8
IPR036869Chaperone J-domain superfamilySUPERFAMILYSSF46565Chaperone J-domaincoord: 20..116
IPR002939Chaperone DnaJ, C-terminalPFAMPF01556DnaJ_Ccoord: 130..322
e-value: 1.2E-33
score: 116.3
NoneNo IPR availableGENE3DG3DSA:2.60.260.20coord: 243..346
e-value: 8.3E-25
score: 88.8
NoneNo IPR availablePANTHERPTHR44298FAMILY NOT NAMEDcoord: 10..351
NoneNo IPR availableCDDcd10747DnaJ_Ccoord: 128..324
e-value: 5.42804E-43
score: 144.49
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..18
score: 0.832
IPR018253DnaJ domain, conserved sitePROSITEPS00636DNAJ_1coord: 63..82
IPR008971HSP40/DnaJ peptide-bindingSUPERFAMILYSSF49493HSP40/DnaJ peptide-binding domaincoord: 124..247
IPR008971HSP40/DnaJ peptide-bindingSUPERFAMILYSSF49493HSP40/DnaJ peptide-binding domaincoord: 245..324