Fukutin-related protein

Overview
NameFukutin-related protein
Smed IDSMED30027343
Length (bp)1157
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Fukutin-related protein (SMED30027343) t-SNE clustered cells

Violin plots show distribution of expression levels for Fukutin-related protein (SMED30027343) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Fukutin-related protein (SMED30027343) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Fukutin-related protein (SMED30027343) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30027343

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X2 cellSMED30027343SMESG000043350.1 SMESG000043348.1 SMESG000043345.1 SmedASXL_013538SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
epidermisSMED30027343SMESG000043350.1 SMESG000043348.1 SMESG000043345.1 dd_Smed_v4_9502_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30027343SMESG000043350.1 SMESG000043348.1 SMESG000043345.1 dd_Smed_v4_9502_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027343SMESG000043350.1 SMESG000043348.1 SMESG000043345.1 SMESG000043344.1 Contig44264newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027343SMESG000043350.1 SMESG000043348.1 SMESG000043345.1 SMESG000043344.1 Contig44264uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Fukutin-related protein vs. Ensembl Zebrafish
Match: fkrp (fukutin related protein [Source:ZFIN;Acc:ZDB-GENE-070412-4])

HSP 1 Score: 51.6026 bits (122), Expect = 1.038e-6
Identity = 21/48 (43.75%), Postives = 31/48 (64.58%), Query Frame = 1 
Query:  475 KTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGI 618
            + L    K  I I   + + +W+  GSL+G+ RHQD+IPWD D+D+GI
Sbjct:  357 RALRETTKYVINILESSGVRYWLEGGSLLGAARHQDIIPWDYDVDLGI 404
[Close]
BLAST of Fukutin-related protein vs. Ensembl Zebrafish
Match: fkrp (fukutin related protein [Source:ZFIN;Acc:ZDB-GENE-070412-4])

HSP 1 Score: 51.6026 bits (122), Expect = 1.038e-6
Identity = 21/48 (43.75%), Postives = 31/48 (64.58%), Query Frame = 1 
Query:  475 KTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGI 618
            + L    K  I I   + + +W+  GSL+G+ RHQD+IPWD D+D+GI
Sbjct:  357 RALRETTKYVINILESSGVRYWLEGGSLLGAARHQDIIPWDYDVDLGI 404
[Close]
BLAST of Fukutin-related protein vs. UniProt/SwissProt
Match: sp|Q68W49|Y683_RICTY (Uncharacterized protein RT0683 OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=RT0683 PE=3 SV=1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.959e-9
Identity = 28/77 (36.36%), Postives = 50/77 (64.94%), Query Frame = 1 
Query:  478 TLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQ----YENVLRQISTPRSEISNDKINII 696
            +L+++MK   E+  +NNI +W+  G+L+G++RHQ +IP+D D+DIGI  +    ++ +L Q       I ++KI +I
Sbjct:  106 SLYQLMKDTHELLTKNNIKYWIESGTLLGAVRHQGIIPFDDDLDIGIMHEDEIHFQQILPQFKQLGYRIKHNKIYVI 182
[Close]
BLAST of Fukutin-related protein vs. UniProt/SwissProt
Match: sp|Q9ZCN5|Y688_RICPR (Uncharacterized protein RP688 OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=RP688 PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 3.553e-9
Identity = 26/58 (44.83%), Postives = 43/58 (74.14%), Query Frame = 1 
Query:  478 TLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQI 651
            +L+++MK   E+  +NNI +W+  G+L+G++RHQ +IP+D D+DIGI  + E  L+QI
Sbjct:  106 SLYQLMKDTHELLTKNNIKYWIESGTLLGAVRHQGIIPFDDDLDIGIMHEDEIHLQQI 163
[Close]
BLAST of Fukutin-related protein vs. UniProt/SwissProt
Match: sp|Q9ZCN4|Y689_RICPR (Uncharacterized protein RP689 OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=RP689 PE=3 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 3.762e-9
Identity = 26/58 (44.83%), Postives = 43/58 (74.14%), Query Frame = 1 
Query:  478 TLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQI 651
            +L+++MK   E+  +NNI +W+  G+L+G++RHQ +IP+D D+DIGI  + E  L+QI
Sbjct:   57 SLYQLMKDTHELLTKNNIKYWIESGTLLGAVRHQGIIPFDDDLDIGIMHEDEIHLQQI 114
[Close]
BLAST of Fukutin-related protein vs. TrEMBL
Match: A0A5J4NGR6 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0005546 PE=4 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 1.256e-45
Identity = 87/211 (41.23%), Postives = 126/211 (59.72%), Query Frame = 1 
Query:  466 NEHKTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRSEISNDKINIITRPGPHCPYN-GMRINCQGKPVTSHEDGCSFCGPFARLFFKPDIRIDIYLFYIQIHENQLQ------ILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYG-DF---KMRSKCVNGKWT 1065
            +  ++L R +K WI     N I WW+ YGSL+GS+R+  MIP+D+D+DI I   +E  LR+++T R  I+  + N++TRPG HC Y+ G R++C GK VTS  D C+FCGP AR+F++    IDIY  ++++  N           DEG +EY P       +  FPL +C  MGL V CPS+ + IL + YG +F   K +    NG+WT
Sbjct:  235 SRRQSLLRTLKHWIRFANDNMIIWWITYGSLLGSVRNGTMIPYDTDMDIAILGSHEAQLRRLATDRQNITPGQFNLVTRPGDHCVYSTGSRVDCTGKQVTSLTDTCAFCGPLARIFYEFGQYIDIYQVHLELRLNSANKPLSFGFYDEGTHEYTPTENLHDASSLFPLSSCRMMGLDVPCPSEPDKILEEVYGAEFIIPKYKCNLTNGEWT 445
[Close]
BLAST of Fukutin-related protein vs. TrEMBL
Match: A0A419Q1H5 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CSKR_8277s PE=4 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 2.693e-45
Identity = 91/223 (40.81%), Postives = 130/223 (58.30%), Query Frame = 1 
Query:  436 SSYDRECGIPNEHK--TLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRSEISNDKINIITRPGPHCPYN-GMRINCQGKPVTSHEDGCSFCGPFARLFFKPDIRIDIYLFYIQIHEN------QLQILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYG-DFKM-RSKCV--NGKWT 1065
            +S  R C +  E +  +L+R ++ WI I  ++ I WW+ YG+L+GS R  +MIP+DSD+DI I   YE  LRQ++T R  I   K N++TRPG HC  + G+R++C G  VTS +D CSFCGP AR+F +    +DIYL ++++  +      +    DEG    M      P +  FPLR C +MGL V CP+  E  L   YG +F + RS+C    G WT
Sbjct:  201 TSLSRPCPVVRESRRESLYRTLQEWIRIANQSGILWWITYGTLLGSWRDGNMIPYDSDVDISILGSYEKRLRQLATDRKSIQKGKFNLVTRPGEHCVTSPGLRLDCDGHNVTSLQDTCSFCGPLARMFHQYGEYVDIYLVHLEVRVDVANNPIEFGYFDEG----MDRDRVEPASVIFPLRTCKYMGLSVPCPNDPERFLTSVYGEEFALPRSRCSQETGSWT 419
[Close]
BLAST of Fukutin-related protein vs. TrEMBL
Match: G7YSX8 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CLF_109802 PE=4 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 2.778e-45
Identity = 91/223 (40.81%), Postives = 130/223 (58.30%), Query Frame = 1 
Query:  436 SSYDRECGIPNEHK--TLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRSEISNDKINIITRPGPHCPYN-GMRINCQGKPVTSHEDGCSFCGPFARLFFKPDIRIDIYLFYIQIHEN------QLQILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYG-DFKM-RSKCV--NGKWT 1065
            +S  R C +  E +  +L+R ++ WI I  ++ I WW+ YG+L+GS R  +MIP+DSD+DI I   YE  LRQ++T R  I   K N++TRPG HC  + G+R++C G  VTS +D CSFCGP AR+F +    +DIYL ++++  +      +    DEG    M      P +  FPLR C +MGL V CP+  E  L   YG +F + RS+C    G WT
Sbjct:  201 TSLSRPCPVVRESRRESLYRTLQEWIRIANQSGILWWITYGTLLGSWRDGNMIPYDSDVDISILGSYEKRLRQLATDRKSIQKGKFNLVTRPGEHCVTSPGLRLDCDGHNVTSLQDTCSFCGPLARMFHQYGEYVDIYLVHLEVRVDVANNPIEFGYFDEG----MDRDRVEPASVIFPLRTCKYMGLSVPCPNDPERFLTSVYGEEFALPRSRCSQETGSWT 419
[Close]
BLAST of Fukutin-related protein vs. TrEMBL
Match: A0A075A604 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_12530 PE=4 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 1.162e-44
Identity = 91/223 (40.81%), Postives = 132/223 (59.19%), Query Frame = 1 
Query:  436 SSYDRECGI--PNEHKTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRSEISNDKINIITRPGPHCPYN-GMRINCQGKPVTSHEDGCSFCGPFARLFFKPDIRIDIYLFYIQIHEN------QLQILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYG-DFKM-RSKCV--NGKWT 1065
            +S  R C I   +  ++L+R ++ WI I  ++ I WW+ YG+L+GS R  +MIP+DSD+DI I   YE  LR+++T R  I   K N++TRPG HC  + G+R++C G+ VTS  D CSFCGP AR+F +    IDIY  ++++  +      +   LDEG    M      P +  FPLRNC +MGL V CP+  E  L   YG +F + +S+C    G+WT
Sbjct:  197 TSLSRPCPIVSASRRESLYRTLQDWIRIANQSKILWWITYGTLLGSWRDGNMIPYDSDVDISILGSYEKRLRELATDRKSIQKGKFNLVTRPGEHCVTSPGLRLDCGGRNVTSLRDTCSFCGPLARMFRQYGEYIDIYPVHLEVRMDVANNPIEFGYLDEG----MDRDRVEPASVIFPLRNCKYMGLNVPCPNDPERFLTSVYGKEFALPKSRCSQETGRWT 415
[Close]
BLAST of Fukutin-related protein vs. TrEMBL
Match: A0A4S2L6M5 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_009663 PE=4 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 5.939e-44
Identity = 88/223 (39.46%), Postives = 134/223 (60.09%), Query Frame = 1 
Query:  436 SSYDRECGI--PNEHKTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRSEISNDKINIITRPGPHCPYN-GMRINCQGKPVTSHEDGCSFCGPFARLFFKPDIRIDIYLFYIQIHEN------QLQILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYG-DFKM-RSKCV--NGKWT 1065
            +S  + C I   +  ++L++ ++ WI++  ++ I WW+ YG+L+GS R  +MIP+DSD+DI I   YE  LR+++T R  I   K N++TRPG HC  + G+R++C G+ VTS +D CSFCGP AR+F +    +DIY  ++++H +      +   LDEG    M      P +  FPLR C +MGL V CP+  E  L   YG +F + +S+C    GKWT
Sbjct:  201 NSLSKPCPIVRASRRESLYQTLQEWIKMANQSKILWWITYGTLLGSWRDGNMIPYDSDVDISILGSYEKRLRELATDRKAIQKGKFNLVTRPGEHCVTSPGLRLDCNGRNVTSLQDTCSFCGPLARMFHQYGEYVDIYPVHLEVHVDVANNPIEFGYLDEG----MDRDRVDPASVIFPLRTCKYMGLSVPCPNDPERFLTSVYGKEFALPKSRCSQETGKWT 419
[Close]
BLAST of Fukutin-related protein vs. Ensembl Cavefish
Match: fkrp (fukutin related protein [Source:NCBI gene;Acc:103033897])

HSP 1 Score: 51.2174 bits (121), Expect = 1.232e-6
Identity = 21/48 (43.75%), Postives = 31/48 (64.58%), Query Frame = 1 
Query:  475 KTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGI 618
            + L    K  I I   + + +W+  GSL+G+ RHQD+IPWD D+D+GI
Sbjct:  363 RALRETTKYVINILDSSGVRYWLEGGSLLGAARHQDIIPWDYDVDLGI 410
[Close]
BLAST of Fukutin-related protein vs. Ensembl Cavefish
Match: fkrp (fukutin related protein [Source:NCBI gene;Acc:103033897])

HSP 1 Score: 50.8322 bits (120), Expect = 1.393e-6
Identity = 21/48 (43.75%), Postives = 31/48 (64.58%), Query Frame = 1 
Query:  475 KTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGI 618
            + L    K  I I   + + +W+  GSL+G+ RHQD+IPWD D+D+GI
Sbjct:  327 RALRETTKYVINILDSSGVRYWLEGGSLLGAARHQDIIPWDYDVDLGI 374
[Close]
BLAST of Fukutin-related protein vs. Ensembl Nematostella
Match: EDO45603 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSB6])

HSP 1 Score: 103.99 bits (258), Expect = 3.211e-25
Identity = 71/218 (32.57%), Postives = 111/218 (50.92%), Query Frame = 1 
Query:  445 DRECGIPNEHKTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRS-----EISNDKINIITRPGPHCPYNG-----MRINCQGKPVTSHEDGCSFCGPFARLFFKPDIRIDIYLFYIQIHENQLQILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYG-DF-KMRSKCVNGKW 1062
            D  C      K L  I++ W EITA++NIT+ +++GSLIG++R  D+IPWD D+D+ +P +   VL ++  PRS           IN I    P   +N      +R +C GK   SH D CS   P  RL+      +D++ +Y Q     +  + EG      + L    N ++P+R C F+G+  +CP+    +L+ +YG +F K    C +G W
Sbjct:  108 DTNCPDNGRRKPLTAILQYWTEITAKHNITYALMWGSLIGAIRTNDVIPWDHDMDLVVPYKDILVLEKLGWPRSSDPYKSDGRTYINAI----PSSEHNKSMDDRLRWDCSGKLTPSHVDLCSIQEPIVRLYNNGAF-LDVFHYYDQ--GETVVTIPEG------HKLEMKRNDFYPIRECLFLGIKSKCPNNPASLLSKWYGLNFIKSPKTCKDGHW 312
[Close]
BLAST of Fukutin-related protein vs. Ensembl Nematostella
Match: EDO31192 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SYN3])

HSP 1 Score: 92.8189 bits (229), Expect = 2.244e-21
Identity = 66/216 (30.56%), Postives = 106/216 (49.07%), Query Frame = 1 
Query:  445 DRECGIPNEHKTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRS--EISNDKINIITRPGPHCPYNG-----MRINCQGKPVTSHEDGCSFCGPFARLFFKPDIRIDIYLFYIQIHENQLQILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYGDFKMRS---KCVNGKW 1062
            D  C        L  ++  W + T  NNI++ +++GSL+G LR  D++ WD DID+ +  +    LR+++      + ++  I++     P   +N       R++C+G+ V    D CSF  P ARL  K    IDIY  Y+++ + +L  L EG    M           FPL+ C +MGL   CP   E +L D+YG   + +   KC +G+W
Sbjct:   76 DTGCFDNEMRAQLTELLNYWNKFTKNNNISYAIMWGSLLGLLRTNDIMAWDYDIDVIVSLKDLVKLRKLAKAEGSFDYNDGNIHLAVMAEPEKSFNTSADKRARLSCRGEAVDKVMDPCSFVDPLARLLLKW-FFIDIYT-YVEVGD-KLFALPEGKRFVMNKAD------MFPLQRCHYMGLECLCPRNPERLLEDWYGPGCVDANEFKCRDGEW 282
[Close]
BLAST of Fukutin-related protein vs. Ensembl Nematostella
Match: EDO45540 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSB7])

HSP 1 Score: 87.0409 bits (214), Expect = 1.136e-19
Identity = 60/211 (28.44%), Postives = 103/211 (48.82%), Query Frame = 1 
Query:  466 NEHKTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLR-----QISTPRS--EISNDKINIITRP-GPHCPY--NGMRINCQGKPVTSHEDGCSFCGPFARLFFKPDIRIDIYLFYIQIHENQLQILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYGDFKMRS--KCVNGKW 1062
             + K L  ++++W  I + +NIT+ ++YGSL+G  R  D++PWD D+DI +   Y+ +L+      I  PR   +I++   +++  P   H  +  +  R +C GK V    D CS   P ARL     + +D Y  Y +  +N + + +     Y    +       FPL+ C  MG+   CP     +L  +YG  ++ S   C +G+W
Sbjct:   51 QKRKLLVTLLRTWNLIASHHNITYSLMYGSLLGVERTADILPWDHDLDIMV--SYDGILKLERIANICDPRGHFDINDGLPHLVVLPNSEHNVFMDDRRRFDCAGKEVAKLVDACSIQEPLARL-IAGSVFMDFY-HYTEKEDNLISVPEGKTVTYAKGEV-------FPLKECFLMGVKSWCPKDPIRVLETWYGKGRLGSHKSCQSGEW 250
[Close]
BLAST of Fukutin-related protein vs. Ensembl Nematostella
Match: EDO38600 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCH0])

HSP 1 Score: 77.0258 bits (188), Expect = 1.784e-15
Identity = 63/220 (28.64%), Postives = 106/220 (48.18%), Query Frame = 1 
Query:  466 NEHKTLFR-IMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRS---EISNDKINIITRPGPHCPYNG------MRINCQGKPVTSHE-DGCSFCGPFARLFFKPDIRIDIYLFYIQIHENQLQILDEGIYEYMPNPL--------YFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYGD-FKMRSK-CVNGKW 1062
            N H+   R ++ SW  + A++ I +++  GSL+G  R++D+IP+DSD+D+ +     + LR I++PR    + ++ K  ++        Y G      +R +C G+ V     D CSF  P ARL  +    +DI+ ++             G+    P P+        YF    +FP++ C+F+G    CP  +E +L   YG+ F    K C NG W
Sbjct:  264 NPHRNTLRDLLASWHLLAAKHKIRYFITSGSLLGYYRNRDIIPYDSDVDVYVDVDSYSTLRSIASPRDFKFKTADSKTRLVV----QMDYWGTPMDSRIRTSCDGRTVDRGSVDPCSFQDPMARLIRRGRY-VDIFAYFTT----------NGVVNTTPTPMYYDPTTKKYFKPEDFFPIKPCTFIGTPTYCPRNAETMLKTMYGEHFATPYKICKNGLW 468
[Close]
BLAST of Fukutin-related protein vs. Ensembl Nematostella
Match: EDO43590 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RY81])

HSP 1 Score: 57.7658 bits (138), Expect = 3.428e-9
Identity = 41/172 (23.84%), Postives = 82/172 (47.67%), Query Frame = 1 
Query:  508 EITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRSEISNDKINIITRPGPHCPYNGMRINCQGKPVTSHEDGCSFCGPFARLFFKPDIRIDIYLFYIQIHENQLQILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYG 1023
            E+   + +++W+  GSL+G++RH D+IPWD D+DIGI   Y++ +R+       +        +R G         I+ +G       +G  F   F+++     + +DI+ FY    E   ++  +  ++     + FP+++  PL    F+G++   P+  +  L   +G
Sbjct:  355 EVLDNSGVSYWLEGGSLLGAVRHSDIIPWDYDVDIGI---YQSQIRKCKPLFYALEQ------SRSGSKF------IDEKGFVWEKSREGEFFRVQFSQI---NHLHVDIFPFY----EKNGKMTKDTWFKTHRQDMEFPVHFLKPLTKIKFVGVLASAPNHIKDFLELKFG 504
[Close]
BLAST of Fukutin-related protein vs. Ensembl Medaka
Match: fkrp (fukutin related protein [Source:NCBI gene;Acc:101164830])

HSP 1 Score: 52.373 bits (124), Expect = 5.609e-7
Identity = 21/48 (43.75%), Postives = 32/48 (66.67%), Query Frame = 1 
Query:  475 KTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGI 618
            + L    K  I I   + + +W+  GSL+G++RHQD+IPWD D+D+GI
Sbjct:  356 RALRETTKYVINILESSGVRYWLEGGSLLGAVRHQDIIPWDYDVDLGI 403
[Close]
BLAST of Fukutin-related protein vs. Planmine SMEST
Match: SMESG000043345.1 (SMESG000043345.1)

HSP 1 Score: 631.328 bits (1627), Expect = 0.000e+0
Identity = 307/307 (100.00%), Postives = 307/307 (100.00%), Query Frame = 1 
Query:  145 MCHLEKCIFPRKQCLLNLTIFVSIVVILIICYSLSENSFITSLDIRIFGARSDEKCLSMKPNKSEPISLESIRKISQLESDKIKIMNENISYQGYMDSSYDRECGIPNEHKTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRSEISNDKINIITRPGPHCPYNGMRINCQGKPVTSHEDGCSFCGPFARLFFKPDIRIDIYLFYIQIHENQLQILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYGDFKMRSKCVNGKWT 1065
            MCHLEKCIFPRKQCLLNLTIFVSIVVILIICYSLSENSFITSLDIRIFGARSDEKCLSMKPNKSEPISLESIRKISQLESDKIKIMNENISYQGYMDSSYDRECGIPNEHKTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRSEISNDKINIITRPGPHCPYNGMRINCQGKPVTSHEDGCSFCGPFARLFFKPDIRIDIYLFYIQIHENQLQILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYGDFKMRSKCVNGKWT
Sbjct:    1 MCHLEKCIFPRKQCLLNLTIFVSIVVILIICYSLSENSFITSLDIRIFGARSDEKCLSMKPNKSEPISLESIRKISQLESDKIKIMNENISYQGYMDSSYDRECGIPNEHKTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRSEISNDKINIITRPGPHCPYNGMRINCQGKPVTSHEDGCSFCGPFARLFFKPDIRIDIYLFYIQIHENQLQILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYGDFKMRSKCVNGKWT 307
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BLAST of Fukutin-related protein vs. Planmine SMEST
Match: SMESG000043345.1 (SMESG000043345.1)

HSP 1 Score: 517.309 bits (1331), Expect = 0.000e+0
Identity = 249/249 (100.00%), Postives = 249/249 (100.00%), Query Frame = 1 
Query:  319 MKPNKSEPISLESIRKISQLESDKIKIMNENISYQGYMDSSYDRECGIPNEHKTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRSEISNDKINIITRPGPHCPYNGMRINCQGKPVTSHEDGCSFCGPFARLFFKPDIRIDIYLFYIQIHENQLQILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYGDFKMRSKCVNGKWT 1065
            MKPNKSEPISLESIRKISQLESDKIKIMNENISYQGYMDSSYDRECGIPNEHKTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRSEISNDKINIITRPGPHCPYNGMRINCQGKPVTSHEDGCSFCGPFARLFFKPDIRIDIYLFYIQIHENQLQILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYGDFKMRSKCVNGKWT
Sbjct:    1 MKPNKSEPISLESIRKISQLESDKIKIMNENISYQGYMDSSYDRECGIPNEHKTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRSEISNDKINIITRPGPHCPYNGMRINCQGKPVTSHEDGCSFCGPFARLFFKPDIRIDIYLFYIQIHENQLQILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYGDFKMRSKCVNGKWT 249
[Close]
BLAST of Fukutin-related protein vs. Planmine SMEST
Match: SMESG000043355.1 (SMESG000043355.1)

HSP 1 Score: 303.908 bits (777), Expect = 1.204e-101
Identity = 133/236 (56.36%), Postives = 176/236 (74.58%), Query Frame = 1 
Query:  358 IRKISQLESDKIKIMNENISYQGYMDSSYDRECGIPNEHKTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRSEISNDKINIITRPGPHCPYNGMRINCQGKPVTSHEDGCSFCGPFARLFFKPDIRIDIYLFYIQIHENQLQILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYGDFKMRSKCVNGKWT 1065
            +  I  +   ++++ N NISY+GY D  + ++CGIP EH+ LF++MK WI+ T+ +NI WW+ YGSLIGS+RH+DM+ WDSDID+G+P+QYE++LR +ST R  ISN ++NIITRPGPHCPYNG R++C+G  VTS  DGC+FCGP AR+F+    RIDIYLF +QIHEN+L+I++EG +EYM      PLNWYFPLRNCSFM L+V CP   E +L DYY  F+   KC  G W 
Sbjct:   76 VTTIGDMTPIQLQLHNSNISYKGYSDLKFSKDCGIPGEHEQLFQLMKFWIKFTSDHNIPWWLAYGSLIGSIRHKDMVAWDSDIDLGMPTQYEHLLRNLSTSRLNISNKQVNIITRPGPHCPYNGPRMDCKGNLVTSQVDGCAFCGPLARVFYNSWKRIDIYLFDVQIHENKLKIVEEGTFEYMSEAPRSPLNWYFPLRNCSFMNLMVLCPKNLEAVLTDYYKTFQSLYKCSKGSWV 311
[Close]
BLAST of Fukutin-related protein vs. Planmine SMEST
Match: SMESG000043350.1 (SMESG000043350.1)

HSP 1 Score: 278.87 bits (712), Expect = 3.972e-94
Identity = 138/143 (96.50%), Postives = 140/143 (97.90%), Query Frame = 1 
Query:  145 MCHLEKCIFPRKQCLLNLTIFVSIVVILIICYSLSENSFITSLDIRIFGARSDEKCLSMKPNKSEPISLESIRKISQLESDKIKIMNENISYQGYMDSSYDRECGIPNEHKTLFRIMKSWIEITARNNITWWMIYGSLIGSLR 573
            MCH EKCIF RKQCLLNL+IFVSIVVILIICYSLSENSFITSLDIRIFGARSDEKCLSMKPN+SEPISLES RKISQLESDKIKIMNENISYQGYMDSSYDRECGIPNEHKTLFRIMKSWIEITARNNITWWMIYGSLIGSLR
Sbjct:    1 MCHFEKCIFQRKQCLLNLSIFVSIVVILIICYSLSENSFITSLDIRIFGARSDEKCLSMKPNESEPISLESNRKISQLESDKIKIMNENISYQGYMDSSYDRECGIPNEHKTLFRIMKSWIEITARNNITWWMIYGSLIGSLR 143
[Close]
BLAST of Fukutin-related protein vs. Planmine SMEST
Match: SMESG000043342.1 (SMESG000043342.1)

HSP 1 Score: 267.314 bits (682), Expect = 1.436e-87
Identity = 124/242 (51.24%), Postives = 161/242 (66.53%), Query Frame = 1 
Query:  337 EPISLESIRKISQLESDKIKIMNENISYQGYMDSSYDRECGIPNEHKTLFRIMKSWIEITARNNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRSEISNDKINIITRPGPHCPYNGMRINCQGKPVTSHEDGCSFCGPFARLFFKPDIRIDIYLFYIQIHENQLQILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGLIVQCPSKSEIILNDYYGDFKMRSKCVNGKW 1062
             PIS+ +  +       + +IMN N+SYQGY+D S+  +C  P  H   F +MK WI+I   NNITWW+I GSLIGS + +DMIPWDSDIDIG+PS++ N++  IST R +IS +K +IITRPGPHCP NGMR++C G PV +  DGCSFCGPFAR+F     RIDI+L Y++IH+NQL++  E ++         P+ W FPL  C FMGL V CP   E I+N  YG FK    C+NG W
Sbjct:   55 NPISVSAPEQGGLFTPQQCQIMNSNVSYQGYLDQSFIGDCSFPANHNEYFDLMKIWIDIAGNNNITWWLISGSLIGSWKFKDMIPWDSDIDIGVPSKFMNLIHNISTDRKDISANKTSIITRPGPHCPRNGMRLDCNGNPVHALVDGCSFCGPFARVFHDHRKRIDIFLSYLEIHDNQLKVSGEEMFNDDLIKRSIPIEWNFPLHQCPFMGLTVPCPKDLEKIINFQYGHFKYDQLCINGTW 296
The following BLAST results are available for this feature:
BLAST of Fukutin-related protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fukutin-related protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fukutin-related protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fukutin-related protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
fkrp1.038e-643.75fukutin related protein [Source:ZFIN;Acc:ZDB-GENE-... [more]
fkrp1.038e-643.75fukutin related protein [Source:ZFIN;Acc:ZDB-GENE-... [more]
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BLAST of Fukutin-related protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fukutin-related protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fukutin-related protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|Q68W49|Y683_RICTY2.959e-936.36Uncharacterized protein RT0683 OS=Rickettsia typhi... [more]
sp|Q9ZCN5|Y688_RICPR3.553e-944.83Uncharacterized protein RP688 OS=Rickettsia prowaz... [more]
sp|Q9ZCN4|Y689_RICPR3.762e-944.83Uncharacterized protein RP689 OS=Rickettsia prowaz... [more]
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BLAST of Fukutin-related protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5J4NGR61.256e-4541.23Uncharacterized protein OS=Paragonimus westermani ... [more]
A0A419Q1H52.693e-4540.81Uncharacterized protein OS=Clonorchis sinensis OX=... [more]
G7YSX82.778e-4540.81Uncharacterized protein OS=Clonorchis sinensis OX=... [more]
A0A075A6041.162e-4440.81Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A4S2L6M55.939e-4439.46Uncharacterized protein OS=Opisthorchis felineus O... [more]
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BLAST of Fukutin-related protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
fkrp1.232e-643.75fukutin related protein [Source:NCBI gene;Acc:1030... [more]
fkrp1.393e-643.75fukutin related protein [Source:NCBI gene;Acc:1030... [more]
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BLAST of Fukutin-related protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fukutin-related protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Fukutin-related protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO456033.211e-2532.57Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO311922.244e-2130.56Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO455401.136e-1928.44Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO386001.784e-1528.64Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO435903.428e-923.84Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Fukutin-related protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
fkrp5.609e-743.75fukutin related protein [Source:NCBI gene;Acc:1011... [more]
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BLAST of Fukutin-related protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000043345.10.000e+0100.00SMESG000043345.1[more]
SMESG000043345.10.000e+0100.00SMESG000043345.1[more]
SMESG000043355.11.204e-10156.36SMESG000043355.1[more]
SMESG000043350.13.972e-9496.50SMESG000043350.1[more]
SMESG000043342.11.436e-8751.24SMESG000043342.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027343 ID=SMED30027343|Name=Fukutin-related protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1157bp
ACAAATTTTCAGGCATGTTCTTGCTTTTCATTGTGAAAATCGGTCGACCA
ATACAATCCAACTCACATTAATATGTATAACACCTGCCGTAAAATTGATT
CAAGTTATTTTCATTTTGGTTTTTCAAATATATTTTTTGTTTTAATGTGC
CACTTAGAAAAATGCATTTTCCCACGCAAACAATGCCTTTTGAACCTGAC
TATATTTGTATCGATAGTTGTAATTTTAATAATCTGTTATAGTCTGTCAG
AAAACAGCTTCATTACGTCATTGGATATTAGGATTTTCGGGGCGAGATCG
GATGAGAAATGCCTTTCCATGAAACCCAATAAATCTGAACCGATTTCTCT
TGAGTCAATTAGGAAAATATCTCAGCTAGAATCGGACAAAATAAAAATTA
TGAATGAAAATATATCTTACCAAGGTTATATGGATAGTTCATATGACAGA
GAATGTGGGATTCCAAATGAGCATAAAACTCTCTTTCGGATAATGAAAAG
TTGGATCGAGATCACGGCGCGTAATAACATAACATGGTGGATGATATATG
GATCACTGATCGGTTCGCTCAGACATCAAGATATGATTCCTTGGGATAGT
GACATTGACATTGGAATACCGAGTCAGTATGAGAATGTCCTTAGACAGAT
AAGCACTCCTCGATCTGAAATTTCAAATGATAAAATAAACATTATCACTA
GACCAGGTCCTCATTGCCCATATAACGGAATGAGAATAAACTGTCAAGGA
AAACCAGTCACTAGTCATGAGGATGGTTGTTCATTCTGTGGACCCTTTGC
TCGATTATTTTTCAAACCTGACATAAGAATAGACATATATTTATTCTACA
TTCAAATTCATGAAAATCAGCTGCAAATATTAGACGAAGGAATCTATGAG
TACATGCCAAATCCTCTTTATTTCCCTCTGAATTGGTACTTTCCCCTTCG
CAACTGCTCTTTCATGGGTCTGATTGTGCAATGCCCAAGCAAATCAGAGA
TAATATTAAATGATTATTATGGTGACTTTAAAATGCGAAGTAAATGTGTA
AATGGAAAATGGACTTAATAACCAAGACGATACATGCTAATTTCATATTT
TATTTGTATATATATACATAATTGATGTACGGATTTATTAAATGCAACGT
TTCTTTA
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protein sequence of SMED30027343-orf-1

>SMED30027343-orf-1 ID=SMED30027343-orf-1|Name=SMED30027343-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=332bp
MYNTCRKIDSSYFHFGFSNIFFVLMCHLEKCIFPRKQCLLNLTIFVSIVV
ILIICYSLSENSFITSLDIRIFGARSDEKCLSMKPNKSEPISLESIRKIS
QLESDKIKIMNENISYQGYMDSSYDRECGIPNEHKTLFRIMKSWIEITAR
NNITWWMIYGSLIGSLRHQDMIPWDSDIDIGIPSQYENVLRQISTPRSEI
SNDKINIITRPGPHCPYNGMRINCQGKPVTSHEDGCSFCGPFARLFFKPD
IRIDIYLFYIQIHENQLQILDEGIYEYMPNPLYFPLNWYFPLRNCSFMGL
IVQCPSKSEIILNDYYGDFKMRSKCVNGKWT*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002032epidermal cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR007074LicD_fam
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007074LicD familyPFAMPF04991LicDcoord: 125..234
e-value: 8.0E-15
score: 55.6
NoneNo IPR availablePANTHERPTHR13627FUKUTIN RELATED PROTEINcoord: 99..299
NoneNo IPR availablePANTHERPTHR13627:SF31FUKUTIN-RELATED PROTEINcoord: 99..299
NoneNo IPR availableTMHMMTMhelixcoord: 13..35