E3 ubiquitin protein ligase
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30027325 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of E3 ubiquitin protein ligase vs. Ensembl Human
Match: RNF40 (ring finger protein 40 [Source:HGNC Symbol;Acc:HGNC:16867]) HSP 1 Score: 305.834 bits (782), Expect = 3.933e-89 Identity = 163/356 (45.79%), Postives = 240/356 (67.42%), Query Frame = 3
Query: 1740 EEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVES---LSYKEEVSRLKSNLEKRLHEPDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYLT 2798
E E++K L++EL ++E KE+ LL+ Y++ K+QRDK QLMA+E + E S E+ K++ D D LRR+R+ EE ++ Q+KL KQ+E+A++++ VT QA+ED+ EQN RLLQ L+EKD+ N KLM++R+KANQ KLL+E++ +Q+ K+Q++A ++ K EEKER +L +EKE T R QALEL+KRKA+E Q AEDL++ L+ ++L+E Q L + +A+ +E+F +R +E++ LRRK E+ +K+E + DE+L EI+ YK LTC C + KKDA+LTKCFHVFC C++ RYE R RKCPKCNA FGA+D+H IY++
Sbjct: 548 ESELLKGLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKAKAEVDELRSRIRELEERDRRESKKIA--DEDALRRIRQAEEQIEHLQRKLGATKQEEEALLSEMDVTGQAFEDMQEQNGRLLQQLREKDDANFKLMSERIKANQIHKLLREEKDELGEQVLGLKSQVDAQLLTVQKLEEKERALQGSLGGVEKELTLRSQALELNKRKAVEAAQLAEDLKVQLEHVQTRLREIQPCLAESRAAREKESFNLKRAQEDISRLRRKLEKQRKVEVYADADEILQEEIKEYKARLTCPCCNTRKKDAVLTKCFHVFCFECVRGRYEARQRKCPKCNAAFGAHDFHRIYIS 901 HSP 2 Score: 105.531 bits (262), Expect = 1.973e-22 Identity = 86/312 (27.56%), Postives = 145/312 (46.47%), Query Frame = 3
Query: 159 EQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLK----------------RLEGDSCE-----------------------VSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEK 974
E ++ G I S EE+D+K LQ +NKKL+E L+Q+Q + ++RIE+LE RQA DD + VNRYW LDE + LL+ EG +C+ +SE A F+ L + E++E +L+ R+ FSK +SR+VEA ++ +R E L ++V S +G + ++E + EL +EN R+ L ++L +HH + E E++DK+ + K+ E ++ ED ++ + + + ++N+ L E E+
Sbjct: 34 EPIRLGGISSTEEMDLKVLQFKNKKLAE-RLEQRQACEDELRERIEKLEKRQATDDATLLIVNRYWAQLDETVEALLRCHESQGELSSAPEAPGTQEGPTCDGTPLPEPGTSELRDPLLMQLRPPLSEPALAFVVALGASSSEEVELELQGRMEFSKAAVSRVVEASDRLQRRVEEL------------------------CQRVYS--------------RGDSEPLSEAAQAHTRELGRENRRLQDLATQLQEKHHRISLEYSELQDKVTSAETKVLEMETTVEDLQWDIEKLRKREQKLNKHLAEALEQ 306 HSP 3 Score: 60.8474 bits (146), Expect = 1.283e-8 Identity = 29/88 (32.95%), Postives = 56/88 (63.64%), Query Frame = 3
Query: 1383 IQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMDNTN 1646
+Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K +AQR ++K +E+QA KL+ +
Sbjct: 337 LQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLISSLQNHNHQLKGDAQRYKRKLREVQAEIGKLRAQASG 424
BLAST of E3 ubiquitin protein ligase vs. Ensembl Human
Match: RNF40 (ring finger protein 40 [Source:HGNC Symbol;Acc:HGNC:16867]) HSP 1 Score: 305.449 bits (781), Expect = 1.755e-88 Identity = 164/361 (45.43%), Postives = 240/361 (66.48%), Query Frame = 3
Query: 1740 EEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE--------PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYLT 2798
E E++K L++EL ++E KE+ LL+ Y++ K+QRDK QLMA+E K EV L+S + + D D LRR+R+ EE ++ Q+KL KQ+E+A++++ VT QA+ED+ EQN RLLQ L+EKD+ N KLM++R+KANQ KLL+E++ +Q+ K+Q++A ++ K EEKER +L +EKE T R QALEL+KRKA+E Q AEDL++ L+ ++L+E Q L + +A+ +E+F +R +E++ LRRK E+ +K+E + DE+L EI+ YK LTC C + KKDA+LTKCFHVFC C++ RYE R RKCPKCNA FGA+D+H IY++
Sbjct: 608 ESELLKGLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKA-------KAEVDELRSRIRELEERDRRESKKIADEDALRRIRQAEEQIEHLQRKLGATKQEEEALLSEMDVTGQAFEDMQEQNGRLLQQLREKDDANFKLMSERIKANQIHKLLREEKDELGEQVLGLKSQVDAQLLTVQKLEEKERALQGSLGGVEKELTLRSQALELNKRKAVEAAQLAEDLKVQLEHVQTRLREIQPCLAESRAAREKESFNLKRAQEDISRLRRKLEKQRKVEVYADADEILQEEIKEYKARLTCPCCNTRKKDAVLTKCFHVFCFECVRGRYEARQRKCPKCNAAFGAHDFHRIYIS 961 HSP 2 Score: 110.923 bits (276), Expect = 4.898e-24 Identity = 53/154 (34.42%), Postives = 100/154 (64.94%), Query Frame = 3
Query: 1185 KLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMDNTN 1646
K+ ++ +PE V+ ++ +Y+ L AQF++LY E++ ++ QL+EAR + + HL+ IE ME +E +Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K +AQR ++K +E+QA KL+ +
Sbjct: 331 KVALRSLPEEVVRETGEYRMLQAQFSLLYNESLQVKTQLDEARGLLLATKNSHLRHIEHMESDELGLQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLISSLQNHNHQLKGDAQRYKRKLREVQAEIGKLRAQASG 484 HSP 3 Score: 105.531 bits (262), Expect = 2.225e-22 Identity = 86/312 (27.56%), Postives = 145/312 (46.47%), Query Frame = 3
Query: 159 EQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLK----------------RLEGDSCE-----------------------VSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEK 974
E ++ G I S EE+D+K LQ +NKKL+E L+Q+Q + ++RIE+LE RQA DD + VNRYW LDE + LL+ EG +C+ +SE A F+ L + E++E +L+ R+ FSK +SR+VEA ++ +R E L ++V S +G + ++E + EL +EN R+ L ++L +HH + E E++DK+ + K+ E ++ ED ++ + + + ++N+ L E E+
Sbjct: 34 EPIRLGGISSTEEMDLKVLQFKNKKLAE-RLEQRQACEDELRERIEKLEKRQATDDATLLIVNRYWAQLDETVEALLRCHESQGELSSAPEAPGTQEGPTCDGTPLPEPGTSELRDPLLMQLRPPLSEPALAFVVALGASSSEEVELELQGRMEFSKAAVSRVVEASDRLQRRVEEL------------------------CQRVYS--------------RGDSEPLSEAAQAHTRELGRENRRLQDLATQLQEKHHRISLEYSELQDKVTSAETKVLEMETTVEDLQWDIEKLRKREQKLNKHLAEALEQ 306
BLAST of E3 ubiquitin protein ligase vs. Ensembl Human
Match: RNF40 (ring finger protein 40 [Source:HGNC Symbol;Acc:HGNC:16867]) HSP 1 Score: 305.449 bits (781), Expect = 3.140e-88 Identity = 164/361 (45.43%), Postives = 240/361 (66.48%), Query Frame = 3
Query: 1740 EEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE--------PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYLT 2798
E E++K L++EL ++E KE+ LL+ Y++ K+QRDK QLMA+E K EV L+S + + D D LRR+R+ EE ++ Q+KL KQ+E+A++++ VT QA+ED+ EQN RLLQ L+EKD+ N KLM++R+KANQ KLL+E++ +Q+ K+Q++A ++ K EEKER +L +EKE T R QALEL+KRKA+E Q AEDL++ L+ ++L+E Q L + +A+ +E+F +R +E++ LRRK E+ +K+E + DE+L EI+ YK LTC C + KKDA+LTKCFHVFC C++ RYE R RKCPKCNA FGA+D+H IY++
Sbjct: 648 ESELLKGLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKA-------KAEVDELRSRIRELEERDRRESKKIADEDALRRIRQAEEQIEHLQRKLGATKQEEEALLSEMDVTGQAFEDMQEQNGRLLQQLREKDDANFKLMSERIKANQIHKLLREEKDELGEQVLGLKSQVDAQLLTVQKLEEKERALQGSLGGVEKELTLRSQALELNKRKAVEAAQLAEDLKVQLEHVQTRLREIQPCLAESRAAREKESFNLKRAQEDISRLRRKLEKQRKVEVYADADEILQEEIKEYKARLTCPCCNTRKKDAVLTKCFHVFCFECVRGRYEARQRKCPKCNAAFGAHDFHRIYIS 1001 HSP 2 Score: 210.69 bits (535), Expect = 1.599e-55 Identity = 156/536 (29.10%), Postives = 272/536 (50.75%), Query Frame = 3
Query: 159 EQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLK----------------RLEGDSCE-----------------------VSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMDNTN 1646
E ++ G I S EE+D+K LQ +NKKL+E L+Q+Q + ++RIE+LE RQA DD + VNRYW LDE + LL+ EG +C+ +SE A F+ L + E++E +L+ R+ FSK +SR+VEA ++ +R E L ++V S +G + ++E + EL +EN R+ L ++L +HH + E E++DK+ + K+ E ++ ED ++ + + + ++N+ L E AL+ + G ++ K + L ELE+ +E R+ ELEKL+ + Q + RLK+ ++ +PE V+ ++ +Y+ L AQF++LY E++ ++ QL+EAR + + HL+ IE ME +E +Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K +AQR ++K +E+QA KL+ +
Sbjct: 34 EPIRLGGISSTEEMDLKVLQFKNKKLAE-RLEQRQACEDELRERIEKLEKRQATDDATLLIVNRYWAQLDETVEALLRCHESQGELSSAPEAPGTQEGPTCDGTPLPEPGTSELRDPLLMQLRPPLSEPALAFVVALGASSSEEVELELQGRMEFSKAAVSRVVEASDRLQRRVEEL------------------------CQRVYS--------------RGDSEPLSEAAQAHTRELGRENRRLQDLATQLQEKHHRISLEYSELQDKVTSAETKVLEMETTVEDLQWDIEKLRKREQKLNKHLAE------ALEQLNSGYYVSGSSSGFQGGQITLSMQKFEMLNAELEENQELANSRMAELEKLQAELQGAVRTNERLKVALRSLPEEVVRETGEYRMLQAQFSLLYNESLQVKTQLDEARGLLLATKNSHLRHIEHMESDELGLQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLISSLQNHNHQLKGDAQRYKRKLREVQAEIGKLRAQASG 524
BLAST of E3 ubiquitin protein ligase vs. Ensembl Human
Match: RNF20 (ring finger protein 20 [Source:HGNC Symbol;Acc:HGNC:10062]) HSP 1 Score: 286.96 bits (733), Expect = 9.052e-82 Identity = 163/358 (45.53%), Postives = 239/358 (66.76%), Query Frame = 3
Query: 1725 DSLTSEEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE-PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
D E E+IK L+ EL ++E KE+ LL+ Y++ K+QRDK QLMA+E + E ++ + L+ +K + D D LR++R +EE ++ Q+KLA+AKQ+E+A++++ VT QA+ED+ EQN RL+Q L+EKD+ N KLM++R+K+NQ KLLKE+++ DQ+ KTQ++A + + K EEKE L +N+ T EKE R QALE++KRKAME Q A+DL+ L+ +L + Q + + + + ++ F +R +E++ LRRK E KK ++ DE+L+ EI+ YK LTC C KKDA+LTKCFHVFC C+KTRY+ R RKCPKCNA FGAND+H IY+
Sbjct: 617 DGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYI 974
BLAST of E3 ubiquitin protein ligase vs. Ensembl Human
Match: RNF40 (ring finger protein 40 [Source:HGNC Symbol;Acc:HGNC:16867]) HSP 1 Score: 96.6709 bits (239), Expect = 2.269e-22 Identity = 66/173 (38.15%), Postives = 105/173 (60.69%), Query Frame = 3
Query: 2196 KLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDM--LTCST-CKSNKKDAILTKCFHVFCLS 2705
KLL+E++ +Q+ K+Q++A ++ K EEKER +L +EKE T R QALEL+KRKA+E Q AEDL++ L+ ++L+E Q L + +A+ +E+F +R +E++ LRRK E+ +K+E + DE+L EI+ YK + CS C D+ + V+ LS
Sbjct: 1 KLLREEKDELGEQVLGLKSQVDAQLLTVQKLEEKERALQGSLGGVEKELTLRSQALELNKRKAVEAAQLAEDLKVQLEHVQTRLREIQPCLAESRAAREKESFNLKRAQEDISRLRRKLEKQRKVEVYADADEILQEEIKEYKGYGRMRCSLQCGGLGLDSSCCQ-LGVWALS 172
BLAST of E3 ubiquitin protein ligase vs. Ensembl Celegans
Match: rfp-1 (E3 ubiquitin-protein ligase bre-1 [Source:UniProtKB/Swiss-Prot;Acc:P34537]) HSP 1 Score: 152.14 bits (383), Expect = 1.566e-37 Identity = 168/612 (27.45%), Postives = 296/612 (48.37%), Query Frame = 3
Query: 1047 SAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEI-PESVILDSVQYKSLHAQFNILYKEAVNMQAQLE-------------EARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMDNTNLXXXXXXXXXXXXRGVLLGLSDPSTFDSLTSEEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHEPDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQ-AYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQA--------LELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREA----FKHRRIKEELMILRRKYERIKKLEH--SNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
S A +++ L E ++ + +RRL +LE++ KK Q +E ++L+L+ Q I++S +YK+L +++ KE + LE EARA + + + H K ++E++ Q+ + + + + LR E+E + E +T+ ++ EM++ ++TLR K+E R R+K K+ Q + LK + T+L++ + +L+ L D +S +++ + Y++ K+ + + E +VE+L ++EV+R + +L E L +RK E L + ++ I+D I A E+ E+N++L +E+++ NLK+M DRM NQT L+E E K Q +A + K E E+ L T ES + A +E H++ E + ++ ++ + Q+K+ +Q+ +A+ARE FK +R +EEL LR KYER+K+ E + + D+VL + K+ LTC +CK+ KD I+ KC+H+FC +C+KT Y+ R RKCPKCN+ FGAND+H I++
Sbjct: 300 SETAAKEIENLRLERDEQESIASRRLQDLEEMNKKVQTLTQENSKLRLETQTFFSVDSIVNSEEYKNLKKYYSLAIKEYERVSKDLEDITTERDAFRSAKEARAMLMS--EEHQKTLKEIQ-----CQSDIHNSFYKVSHDSEVLRCEFETVKEEYNKTVKQSEWD-----EMKATLNTLRSMNRSLKSEKIRLREKDKQSQKDINTLKSELTSLKEAQD-------KCLLVPLED----------------------VSNAPPEDVNKIRQEYESLCKEVKRLGAMEKQEKQKQVENL--QKEVNR---QIADKLSE-----LETLRKTNEMLTN-----------DEECISDELEAIGTAVEEEQERNAQLYIEKREQEDRNLKMMNDRMIQNQTFNRLREKLSCLES-----KAQTDA---QIAKMHEFEKKANEELVTKLSESVQFKSAELTRLTNLMEQHRKNIQEVGMSRDENQIKADRCEGQMKQ----IQELYAAKAREIEDFKFKRQRAEEELETLRIKYERVKRNESVPAQSGDQVLEEANRQMKETLTCPSCKTRPKDCIMLKCYHLFCETCIKTMYDTRQRKCPKCNSNFGANDFHRIFI 837
BLAST of E3 ubiquitin protein ligase vs. Ensembl Celegans
Match: rfp-1 (E3 ubiquitin-protein ligase bre-1 [Source:UniProtKB/Swiss-Prot;Acc:P34537]) HSP 1 Score: 149.443 bits (376), Expect = 1.176e-36 Identity = 167/612 (27.29%), Postives = 293/612 (47.88%), Query Frame = 3
Query: 1047 SAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEI-PESVILDSVQYKSLHAQFNILYKEAVNMQAQLE-------------EARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMDNTNLXXXXXXXXXXXXRGVLLGLSDPSTFDSLTSEEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHEPDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQ-AYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQA--------LELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREA----FKHRRIKEELMILRRKYERIKKLEH--SNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
S A +++ L E ++ + +RRL +LE++ KK Q +E ++L+L+ Q I++S +YK+L +++ KE + LE EARA + + + H K ++E++ Q+ + + + + LR E+E + E +T+ ++ EM++ ++TLR K+E R R+K K+ Q + LK + T+L++ + +L+ L D +S +++ + Y++ K+ + + E +VE +EV+R + +L E L +RK E L + ++ I+D I A E+ E+N++L +E+++ NLK+M DRM NQT L+E E K Q +A + K E E+ L T ES + A +E H++ E + ++ ++ + Q+K+ +Q+ +A+ARE FK +R +EEL LR KYER+K+ E + + D+VL + K+ LTC +CK+ KD I+ KC+H+FC +C+KT Y+ R RKCPKCN+ FGAND+H I++
Sbjct: 300 SETAAKEIENLRLERDEQESIASRRLQDLEEMNKKVQTLTQENSKLRLETQTFFSVDSIVNSEEYKNLKKYYSLAIKEYERVSKDLEDITTERDAFRSAKEARAMLMS--EEHQKTLKEIQ-----CQSDIHNSFYKVSHDSEVLRCEFETVKEEYNKTVKQSEWD-----EMKATLNTLRSMNRSLKSEKIRLREKDKQSQKDINTLKSELTSLKEAQD-------KCLLVPLED----------------------VSNAPPEDVNKIRQEYESLCKEVKRLGAMEKQEKQKQVE-----KEVNR---QIADKLSE-----LETLRKTNEMLTN-----------DEECISDELEAIGTAVEEEQERNAQLYIEKREQEDRNLKMMNDRMIQNQTFNRLREKLSCLES-----KAQTDA---QIAKMHEFEKKANEELVTKLSESVQFKSAELTRLTNLMEQHRKNIQEVGMSRDENQIKADRCEGQMKQ----IQELYAAKAREIEDFKFKRQRAEEELETLRIKYERVKRNESVPAQSGDQVLEEANRQMKETLTCPSCKTRPKDCIMLKCYHLFCETCIKTMYDTRQRKCPKCNSNFGANDFHRIFI 834
BLAST of E3 ubiquitin protein ligase vs. Ensembl Fly
Match: Bre1 (gene:FBgn0086694 transcript:FBtr0077116) HSP 1 Score: 318.546 bits (815), Expect = 4.831e-93 Identity = 171/377 (45.36%), Postives = 255/377 (67.64%), Query Frame = 3
Query: 1740 EEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLK-SNLEKRLHEPDPDQLRRVRKMEETLKDYQQKLA-----------------------LAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNT-DEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
E E +++L+++L + KE+ LL+ Y+ +KDQRDK QLMA+E +R E ++++ +L+ S E+R D + LR+++++EE + Q+++A + +E+A++N+ VT QA+ED+ EQNSRL+Q L+EKD+ N KLM++R+KANQ KLL+E++ + EDQ+ TQIEA++ L K EEKER + ++EKE RQQA+E+HKRKA+E Q+A DL+++L+KY +Q+KE+Q + +K S+ EA+K +R++EEL +RK ER+KK+E S T DEV+I EI+ YK+ LTC +CK +KDA+L+KCFHVFC CL+TRYE R RKCPKCN FGANDYH +YL
Sbjct: 667 ESETVRDLKAQLKKALNDQKEMKLLLDMYKGVSKDQRDKVQLMATEKKLRSEIEELRQQLKKLQESKREERKKLADEEALRKIKQLEEQKYELQKQMANHKPTDNSWGSGAPGTANYTRPFVGSHEEEALLNEMEVTGQAFEDMQEQNSRLIQQLREKDDANFKLMSERIKANQLHKLLREEKTVLEDQMATATTQIEAMHIVLRKLEEKERSLQATVASIEKELMLRQQAMEMHKRKAIESAQSAADLKLHLEKYHAQMKEAQQVVAEKTSSLEAEAYKTKRLQEELAQFKRKAERMKKMEMSGTTIDEVMIEEIREYKETLTCPSCKVKRKDAVLSKCFHVFCYDCLRTRYETRQRKCPKCNCAFGANDYHRLYL 1043 HSP 2 Score: 221.09 bits (562), Expect = 3.150e-59 Identity = 167/546 (30.59%), Postives = 289/546 (52.93%), Query Frame = 3
Query: 72 MSKRPADFDETAND-----ETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNHKDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLEGDSCEV------SETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHEL--------------KSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKM 1634
MSKR AD D T + P+K+ +E G + + EE+D+K L+ QNKKL++ + + + + RIEQLE RQ +DD ++ VNRYWN L+ED ++LL+R + ++ + +E FL QL++WDKE+L+ KL RV SKR ++++V+ ++ +R E + +H +K + + + E ++ + V + E LK E+ EN ++ L + LH + H + ++EN E+K++++ LE+ + + E LAE ++ + N+ + K T +++ ++EL ELE+ +E RL EL+KL H+ L+EV +LK+ ++++PESVI+++ +YK L +QF++LY E++ ++ L+E R +QT++ HL+QIE ME E + Q ++RS+M+ M + + +R EYE L +E +Q + N+ + PI EMR LI +L+ Q K E QR ++KYK+ + D LK+
Sbjct: 1 MSKRSAD-DATGSSCLVAAAAAGQPPIKKV--HFEPHLIGPVSTLEEMDIKVLEFQNKKLAQRIEQRMRTEAELRHRIEQLEKRQTQDDAVLNVVNRYWNQLNEDIRVLLQRFDAETADELENRNENEVTTSFLAQLSTWDKEELDEKLANRVQVSKRAVAKIVQVIDRLMQRNEKI------THVLKGDSLASAGSGSGAGAGGEEEQQQASGDAETTTSSAGVHALEETLKQTHIEIMSENHKLQNLNTSLHEKFHTMSLKMKEYQDAHTAKETENAELKNQIDELQYDLEKIHCRNDKLENHLAEAIEKLKAYHQIYGDPNKSTNSAK--------TPTTTGSGGATTSVNSQLLEELQKELEEYRELANNRLQELDKLHATHRETLKEVEKLKMDIRQLPESVIVETTEYKCLQSQFSVLYNESMQIKTMLDETRNQLQTSKNQHLRQIEVMESEELIAQKKVRSEMIQMEDVLALIRKEYETLRIEFEQNMAANEQTAPINREMRHLITSLQNHNGQLKGEVQRYKRKYKD--TSTDNLKL 527
BLAST of E3 ubiquitin protein ligase vs. Ensembl Fly
Match: Bre1 (gene:FBgn0086694 transcript:FBtr0344538) HSP 1 Score: 318.546 bits (815), Expect = 4.831e-93 Identity = 171/377 (45.36%), Postives = 255/377 (67.64%), Query Frame = 3
Query: 1740 EEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLK-SNLEKRLHEPDPDQLRRVRKMEETLKDYQQKLA-----------------------LAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNT-DEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
E E +++L+++L + KE+ LL+ Y+ +KDQRDK QLMA+E +R E ++++ +L+ S E+R D + LR+++++EE + Q+++A + +E+A++N+ VT QA+ED+ EQNSRL+Q L+EKD+ N KLM++R+KANQ KLL+E++ + EDQ+ TQIEA++ L K EEKER + ++EKE RQQA+E+HKRKA+E Q+A DL+++L+KY +Q+KE+Q + +K S+ EA+K +R++EEL +RK ER+KK+E S T DEV+I EI+ YK+ LTC +CK +KDA+L+KCFHVFC CL+TRYE R RKCPKCN FGANDYH +YL
Sbjct: 667 ESETVRDLKAQLKKALNDQKEMKLLLDMYKGVSKDQRDKVQLMATEKKLRSEIEELRQQLKKLQESKREERKKLADEEALRKIKQLEEQKYELQKQMANHKPTDNSWGSGAPGTANYTRPFVGSHEEEALLNEMEVTGQAFEDMQEQNSRLIQQLREKDDANFKLMSERIKANQLHKLLREEKTVLEDQMATATTQIEAMHIVLRKLEEKERSLQATVASIEKELMLRQQAMEMHKRKAIESAQSAADLKLHLEKYHAQMKEAQQVVAEKTSSLEAEAYKTKRLQEELAQFKRKAERMKKMEMSGTTIDEVMIEEIREYKETLTCPSCKVKRKDAVLSKCFHVFCYDCLRTRYETRQRKCPKCNCAFGANDYHRLYL 1043 HSP 2 Score: 221.09 bits (562), Expect = 3.150e-59 Identity = 167/546 (30.59%), Postives = 289/546 (52.93%), Query Frame = 3
Query: 72 MSKRPADFDETAND-----ETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNHKDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLEGDSCEV------SETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHEL--------------KSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKM 1634
MSKR AD D T + P+K+ +E G + + EE+D+K L+ QNKKL++ + + + + RIEQLE RQ +DD ++ VNRYWN L+ED ++LL+R + ++ + +E FL QL++WDKE+L+ KL RV SKR ++++V+ ++ +R E + +H +K + + + E ++ + V + E LK E+ EN ++ L + LH + H + ++EN E+K++++ LE+ + + E LAE ++ + N+ + K T +++ ++EL ELE+ +E RL EL+KL H+ L+EV +LK+ ++++PESVI+++ +YK L +QF++LY E++ ++ L+E R +QT++ HL+QIE ME E + Q ++RS+M+ M + + +R EYE L +E +Q + N+ + PI EMR LI +L+ Q K E QR ++KYK+ + D LK+
Sbjct: 1 MSKRSAD-DATGSSCLVAAAAAGQPPIKKV--HFEPHLIGPVSTLEEMDIKVLEFQNKKLAQRIEQRMRTEAELRHRIEQLEKRQTQDDAVLNVVNRYWNQLNEDIRVLLQRFDAETADELENRNENEVTTSFLAQLSTWDKEELDEKLANRVQVSKRAVAKIVQVIDRLMQRNEKI------THVLKGDSLASAGSGSGAGAGGEEEQQQASGDAETTTSSAGVHALEETLKQTHIEIMSENHKLQNLNTSLHEKFHTMSLKMKEYQDAHTAKETENAELKNQIDELQYDLEKIHCRNDKLENHLAEAIEKLKAYHQIYGDPNKSTNSAK--------TPTTTGSGGATTSVNSQLLEELQKELEEYRELANNRLQELDKLHATHRETLKEVEKLKMDIRQLPESVIVETTEYKCLQSQFSVLYNESMQIKTMLDETRNQLQTSKNQHLRQIEVMESEELIAQKKVRSEMIQMEDVLALIRKEYETLRIEFEQNMAANEQTAPINREMRHLITSLQNHNGQLKGEVQRYKRKYKD--TSTDNLKL 527
BLAST of E3 ubiquitin protein ligase vs. Ensembl Zebrafish
Match: rnf20 (ring finger protein 20, E3 ubiquitin protein ligase [Source:ZFIN;Acc:ZDB-GENE-130404-2]) HSP 1 Score: 265.388 bits (677), Expect = 3.307e-74 Identity = 155/356 (43.54%), Postives = 233/356 (65.45%), Query Frame = 3
Query: 1740 EEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVES---LSYKEEVSRLKSNLEKRLHEPDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKK-LEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
E E +K ++ EL ++E +E+ LL+ Y++ K+QRDK QLMA+E + E+ E+ + K++ D + LR++R +EE + +KL+LAKQ+EDA++++ VT QA+ED+ EQN RL+Q L+EKD+ N KLM++R+K+NQ KLLKE+++ DQ+ KTQ++A + + K EEKERL ++ E+E R QALE++KRKA + +E++R L+ +L+ + + + + ++ +E+F RR +E++ LRRK E+ KK E+ N DE+L EI YK LTC C S KDA+LTKCFHVFC C+KTRY+ R RKCPKCNA FGAND+H IY+
Sbjct: 659 EMEQLKIVRVELKKAQESQREMKLLLDMYRSAPKEQRDKVQLMAAEKKTKSEAEELRQRVRELEERERREGKKM--ADEEALRKIRSVEEQIDILNKKLSLAKQEEDALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQLLTLKTQVDAQLQVVRKLEEKERLLQGTISAAERELGLRTQALEMNKRKAQDSVLLSEEVRTQLEGVQQRLRSVREEVIENSISREKESFNARRAQEDISKLRRKIEKAKKPAENIRNGDEILNEEINEYKARLTCPCCNSRVKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYI 1012 HSP 2 Score: 180.259 bits (456), Expect = 6.826e-46 Identity = 149/528 (28.22%), Postives = 262/528 (49.62%), Query Frame = 3
Query: 165 VKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLE-------------------------GDS--------CEVSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMDNTN 1646
+K G + EE D+K LQI+N+KL E L DQ+Q + + ++R+E LE RQA DD + +NRYWN DE ++ ++R + GDS + E FL LAS E+++ +L+ERV S + SR+VE +E NL++ VD +K + +ES + G+ D+ ++L + L N R+ +L L +H + SE+ + ++ E + E+ ++ + + R NR+N L E E+ + ++ G I K K +E+ +ELE+ +E RL+EL+KL++ Q +E T +K+++ E V DS +Y+ L +QF++LY E++ +++QL+E +A + T R L+Q++ ME +E +Q ++R++++ + + +++R EYE L +E +QT+ N+ + PI EMR LI TL+ Q K E + + + +E Q ++L+ N
Sbjct: 46 IKLGGGANLEEQDIKALQIKNRKLGEAL-DQRQVIEDELRERVETLETRQATDDASLLILNRYWNQFDEHVRLFIRRCDQSGSEPVESQPPEGRSLKPGTPEPDGDSNQERAKDRGQQGEGVSSFLATLASSTSEEIDAELQERVESSCKQASRVVEIYE-------NLKN----------------MVDQLK-KDLESGKDG-----------GLWDVA---VRLN-ALLTGMNERLCQLTDGLQQKHSHMTSESRSLGRAANRADNRISELQGLIEELQWDMEKIRRRENRLNTHLVEVLERVNSKGYKVC------GEASSVCGTITINKRKFEEMNSELEENRELAENRLSELQKLQQDLQTVYQENTNMKVELLSRAEEVARDSAEYRCLQSQFSVLYNESLVLKSQLDETKARLNTTRTARLRQLDHMENDEVSLQRKVRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISTLQTHNQQMKGEVVKYKLRLREAQQELNQLRAAKGN 527
BLAST of E3 ubiquitin protein ligase vs. Ensembl Zebrafish
Match: rnf20 (ring finger protein 20, E3 ubiquitin protein ligase [Source:ZFIN;Acc:ZDB-GENE-130404-2]) HSP 1 Score: 265.388 bits (677), Expect = 3.307e-74 Identity = 155/356 (43.54%), Postives = 233/356 (65.45%), Query Frame = 3
Query: 1740 EEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVES---LSYKEEVSRLKSNLEKRLHEPDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKK-LEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
E E +K ++ EL ++E +E+ LL+ Y++ K+QRDK QLMA+E + E+ E+ + K++ D + LR++R +EE + +KL+LAKQ+EDA++++ VT QA+ED+ EQN RL+Q L+EKD+ N KLM++R+K+NQ KLLKE+++ DQ+ KTQ++A + + K EEKERL ++ E+E R QALE++KRKA + +E++R L+ +L+ + + + + ++ +E+F RR +E++ LRRK E+ KK E+ N DE+L EI YK LTC C S KDA+LTKCFHVFC C+KTRY+ R RKCPKCNA FGAND+H IY+
Sbjct: 659 EMEQLKIVRVELKKAQESQREMKLLLDMYRSAPKEQRDKVQLMAAEKKTKSEAEELRQRVRELEERERREGKKM--ADEEALRKIRSVEEQIDILNKKLSLAKQEEDALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQLLTLKTQVDAQLQVVRKLEEKERLLQGTISAAERELGLRTQALEMNKRKAQDSVLLSEEVRTQLEGVQQRLRSVREEVIENSISREKESFNARRAQEDISKLRRKIEKAKKPAENIRNGDEILNEEINEYKARLTCPCCNSRVKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYI 1012 HSP 2 Score: 180.259 bits (456), Expect = 6.826e-46 Identity = 149/528 (28.22%), Postives = 262/528 (49.62%), Query Frame = 3
Query: 165 VKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLE-------------------------GDS--------CEVSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMDNTN 1646
+K G + EE D+K LQI+N+KL E L DQ+Q + + ++R+E LE RQA DD + +NRYWN DE ++ ++R + GDS + E FL LAS E+++ +L+ERV S + SR+VE +E NL++ VD +K + +ES + G+ D+ ++L + L N R+ +L L +H + SE+ + ++ E + E+ ++ + + R NR+N L E E+ + ++ G I K K +E+ +ELE+ +E RL+EL+KL++ Q +E T +K+++ E V DS +Y+ L +QF++LY E++ +++QL+E +A + T R L+Q++ ME +E +Q ++R++++ + + +++R EYE L +E +QT+ N+ + PI EMR LI TL+ Q K E + + + +E Q ++L+ N
Sbjct: 46 IKLGGGANLEEQDIKALQIKNRKLGEAL-DQRQVIEDELRERVETLETRQATDDASLLILNRYWNQFDEHVRLFIRRCDQSGSEPVESQPPEGRSLKPGTPEPDGDSNQERAKDRGQQGEGVSSFLATLASSTSEEIDAELQERVESSCKQASRVVEIYE-------NLKN----------------MVDQLK-KDLESGKDG-----------GLWDVA---VRLN-ALLTGMNERLCQLTDGLQQKHSHMTSESRSLGRAANRADNRISELQGLIEELQWDMEKIRRRENRLNTHLVEVLERVNSKGYKVC------GEASSVCGTITINKRKFEEMNSELEENRELAENRLSELQKLQQDLQTVYQENTNMKVELLSRAEEVARDSAEYRCLQSQFSVLYNESLVLKSQLDETKARLNTTRTARLRQLDHMENDEVSLQRKVRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISTLQTHNQQMKGEVVKYKLRLREAQQELNQLRAAKGN 527
BLAST of E3 ubiquitin protein ligase vs. Ensembl Zebrafish
Match: rnf40 (ring finger protein 40 [Source:ZFIN;Acc:ZDB-GENE-030131-6607]) HSP 1 Score: 148.288 bits (373), Expect = 6.723e-36 Identity = 117/404 (28.96%), Postives = 214/404 (52.97%), Query Frame = 3
Query: 420 ETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLK 1631
E+A+ FL L + +E+L +L++R+ FSK ++ +V F++ + + L ++ VES D++ D+ N KL +EN R+ L S L +HH++ E E+ DK+ + K+ E ++ ED ++ + + + ++N+ L E AL+ + P G ++ K + L ELE +E R+ ELEKL+++ Q + E +LK+ ++ IPE V+ ++ +YK L +QF++LY E+++++ QL+EARA + T + HL+QIE ME +E +Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K + QR ++K +E Q +KLK
Sbjct: 224 ESAKAFLAILDNSSEEELTLQLQDRMQFSKGAVACMVCIFDRLHTSIDKLCTR------------------------VESAACEDDSQR---------DIATTNRKL-----LEENNRLYDLASSLQGKHHKMSLEYNELVDKVTSSETKVSEMETAVEDLQWDIEKLRKREQKLNKHLAE------ALEQLNTGYHATGSSGGLPGGQITLSIQKFESLNAELEQNQELANSRMAELEKLQQELQEAVREGEKLKMDLRNIPEEVLKETPEYKCLQSQFSLLYNESLSVKTQLDEARALLLTAKNAHLRQIEHMESDELSLQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLISSLQNHNHQLKGDVQRYKRKLRETQMEINKLK 583 HSP 2 Score: 68.5514 bits (166), Expect = 3.459e-11 Identity = 43/116 (37.07%), Postives = 64/116 (55.17%), Query Frame = 3
Query: 75 SKRPADFDETANDETCPNIPVKQFVSS-------YEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLE 398
KRP+ D + P P K+ E ++ G + S EE+DMK +Q +NKKL E L+Q+Q L + +++IE+LE RQA DD + VNR W L+E+ LL+ +E
Sbjct: 13 GKRPSGGD------SPPGPPEKKLKKEEKTTTTLIEPIRIGGVSSTEEMDMKVIQFKNKKLCE-RLEQRQALEDELREKIEKLEKRQATDDTTLLIVNRCWTQLEENIHELLQHVE 121
BLAST of E3 ubiquitin protein ligase vs. Ensembl Zebrafish
Match: rnf40 (ring finger protein 40 [Source:ZFIN;Acc:ZDB-GENE-030131-6607]) HSP 1 Score: 148.288 bits (373), Expect = 6.833e-36 Identity = 117/404 (28.96%), Postives = 214/404 (52.97%), Query Frame = 3
Query: 420 ETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLK 1631
E+A+ FL L + +E+L +L++R+ FSK ++ +V F++ + + L ++ VES D++ D+ N KL +EN R+ L S L +HH++ E E+ DK+ + K+ E ++ ED ++ + + + ++N+ L E AL+ + P G ++ K + L ELE +E R+ ELEKL+++ Q + E +LK+ ++ IPE V+ ++ +YK L +QF++LY E+++++ QL+EARA + T + HL+QIE ME +E +Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K + QR ++K +E Q +KLK
Sbjct: 218 ESAKAFLAILDNSSEEELTLQLQDRMQFSKGAVACMVCIFDRLHTSIDKLCTR------------------------VESAACEDDSQR---------DIATTNRKL-----LEENNRLYDLASSLQGKHHKMSLEYNELVDKVTSSETKVSEMETAVEDLQWDIEKLRKREQKLNKHLAE------ALEQLNTGYHATGSSGGLPGGQITLSIQKFESLNAELEQNQELANSRMAELEKLQQELQEAVREGEKLKMDLRNIPEEVLKETPEYKCLQSQFSLLYNESLSVKTQLDEARALLLTAKNAHLRQIEHMESDELSLQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLISSLQNHNHQLKGDVQRYKRKLRETQMEINKLK 577 HSP 2 Score: 68.5514 bits (166), Expect = 3.540e-11 Identity = 43/116 (37.07%), Postives = 64/116 (55.17%), Query Frame = 3
Query: 75 SKRPADFDETANDETCPNIPVKQFVSS-------YEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLE 398
KRP+ D + P P K+ E ++ G + S EE+DMK +Q +NKKL E L+Q+Q L + +++IE+LE RQA DD + VNR W L+E+ LL+ +E
Sbjct: 7 GKRPSGGD------SPPGPPEKKLKKEEKTTTTLIEPIRIGGVSSTEEMDMKVIQFKNKKLCE-RLEQRQALEDELREKIEKLEKRQATDDTTLLIVNRCWTQLEENIHELLQHVE 115
BLAST of E3 ubiquitin protein ligase vs. Ensembl Zebrafish
Match: rnf40 (ring finger protein 40 [Source:ZFIN;Acc:ZDB-GENE-030131-6607]) HSP 1 Score: 147.517 bits (371), Expect = 9.616e-36 Identity = 117/404 (28.96%), Postives = 214/404 (52.97%), Query Frame = 3
Query: 420 ETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLK 1631
E+A+ FL L + +E+L +L++R+ FSK ++ +V F++ + + L ++ VES D++ D+ N KL +EN R+ L S L +HH++ E E+ DK+ + K+ E ++ ED ++ + + + ++N+ L E AL+ + P G ++ K + L ELE +E R+ ELEKL+++ Q + E +LK+ ++ IPE V+ ++ +YK L +QF++LY E+++++ QL+EARA + T + HL+QIE ME +E +Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K + QR ++K +E Q +KLK
Sbjct: 139 ESAKAFLAILDNSSEEELTLQLQDRMQFSKGAVACMVCIFDRLHTSIDKLCTR------------------------VESAACEDDSQR---------DIATTNRKL-----LEENNRLYDLASSLQGKHHKMSLEYNELVDKVTSSETKVSEMETAVEDLQWDIEKLRKREQKLNKHLAE------ALEQLNTGYHATGSSGGLPGGQITLSIQKFESLNAELEQNQELANSRMAELEKLQQELQEAVREGEKLKMDLRNIPEEVLKETPEYKCLQSQFSLLYNESLSVKTQLDEARALLLTAKNAHLRQIEHMESDELSLQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLISSLQNHNHQLKGDVQRYKRKLRETQMEINKLK 498
BLAST of E3 ubiquitin protein ligase vs. Ensembl Xenopus
Match: mphosph10 (M-phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein) [Source:Xenbase;Acc:XB-GENE-940335]) HSP 1 Score: 290.041 bits (741), Expect = 5.154e-84 Identity = 156/354 (44.07%), Postives = 243/354 (68.64%), Query Frame = 3
Query: 1746 EMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE----PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
+++K L++EL ++E KE+ LL+ Y++ K+QRDK QLMA+E + E +E+ +R+++ EK E D + LR+++ EE + Q+KL KQ+E+A++++ VT QA+ED+ EQN RL+Q L+EKD+ N KLM++R+K+NQ KLL+E+++ +Q++ K Q++A + K EE+ERL +L ++EKE T R Q+LEL+KRKA+E Q AEDL++ ++ L+++Q + + SA+ +E+F +R +E++ LRRK E+ KK+E + D++L EI+ Y+ LTC C + KKDA+LTKCFHVFC C+KTRYE R RKCPKCNA FGA+D+H IY+
Sbjct: 481 DILKQLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKTKAE---MEEQRTRVQALEEKDKRERKKLADEEALRKIKMAEEQIDHLQRKLTATKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLREEKEELAEQVQGLKAQVDAQLLQVQKLEERERLLHTSLTSIEKEVTLRTQSLELNKRKAVEAAQLAEDLKVQMEHVGETLRDTQNFVCENRSAKEKESFSLKRAQEDVSRLRRKLEKQKKMEVYADADQILQEEIKEYRARLTCPCCNTRKKDAVLTKCFHVFCFECVKTRYESRQRKCPKCNAAFGAHDFHRIYI 831 HSP 2 Score: 107.071 bits (266), Expect = 5.643e-23 Identity = 89/311 (28.62%), Postives = 145/311 (46.62%), Query Frame = 3
Query: 75 SKRPADFDETANDETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNML----------DEDCQILLKRLEGDSCEVSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEK 974
SKR + + + E PN+ K + E ++ G I S EE+DMK LQ +NKKL+E L+Q+Q + +++IE+LE RQA DD + VNRYW+ + DE + + E++ FL LA KE++E KL+ER+ FSK+ +S ++E K + R E L + I +G D + E+ K EL EN R+ L + L +HH L ++ E++D+ + K+ E + ED ++ + + + ++NR L E E+
Sbjct: 6 SKRASSDNGSGPSEKKPNLEAKTPTTLIEPIRLGGISSTEEMDMKVLQFKNKKLAE-RLEQRQGFEDELREKIEKLEKRQATDDATLLIVNRYWSKVESAKETAMETDETSPEPPPAPPFSTGALPESSLTFLATLAHCTKEEIELKLQERMEFSKKAVSCVLETSNKLHCRTEELCDR---------------------------------------IHKGDFDHLGESPKKLNKELITENRRLQDLATVLQEKHHRLSLQHHELQDRATSTETKVSEMERTIEDLQWDIEKLRKHEQKLNRHLAEALEQ 276 HSP 3 Score: 56.9954 bits (136), Expect = 1.597e-7 Identity = 27/84 (32.14%), Postives = 53/84 (63.10%), Query Frame = 3
Query: 1383 IQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKM 1634
+Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K + QR ++K ++ QA KL+
Sbjct: 292 VQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLIGSLQNHNHQLKGDVQRYKRKVRDSQAEVSKLRF 375
BLAST of E3 ubiquitin protein ligase vs. Ensembl Xenopus
Match: mphosph10 (M-phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein) [Source:Xenbase;Acc:XB-GENE-940335]) HSP 1 Score: 289.656 bits (740), Expect = 2.389e-83 Identity = 156/354 (44.07%), Postives = 243/354 (68.64%), Query Frame = 3
Query: 1746 EMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE----PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
+++K L++EL ++E KE+ LL+ Y++ K+QRDK QLMA+E + E +E+ +R+++ EK E D + LR+++ EE + Q+KL KQ+E+A++++ VT QA+ED+ EQN RL+Q L+EKD+ N KLM++R+K+NQ KLL+E+++ +Q++ K Q++A + K EE+ERL +L ++EKE T R Q+LEL+KRKA+E Q AEDL++ ++ L+++Q + + SA+ +E+F +R +E++ LRRK E+ KK+E + D++L EI+ Y+ LTC C + KKDA+LTKCFHVFC C+KTRYE R RKCPKCNA FGA+D+H IY+
Sbjct: 543 DILKQLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKTKAE---MEEQRTRVQALEEKDKRERKKLADEEALRKIKMAEEQIDHLQRKLTATKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLREEKEELAEQVQGLKAQVDAQLLQVQKLEERERLLHTSLTSIEKEVTLRTQSLELNKRKAVEAAQLAEDLKVQMEHVGETLRDTQNFVCENRSAKEKESFSLKRAQEDVSRLRRKLEKQKKMEVYADADQILQEEIKEYRARLTCPCCNTRKKDAVLTKCFHVFCFECVKTRYESRQRKCPKCNAAFGAHDFHRIYI 893 HSP 2 Score: 107.071 bits (266), Expect = 6.066e-23 Identity = 89/311 (28.62%), Postives = 145/311 (46.62%), Query Frame = 3
Query: 75 SKRPADFDETANDETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNML----------DEDCQILLKRLEGDSCEVSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEK 974
SKR + + + E PN+ K + E ++ G I S EE+DMK LQ +NKKL+E L+Q+Q + +++IE+LE RQA DD + VNRYW+ + DE + + E++ FL LA KE++E KL+ER+ FSK+ +S ++E K + R E L + I +G D + E+ K EL EN R+ L + L +HH L ++ E++D+ + K+ E + ED ++ + + + ++NR L E E+
Sbjct: 6 SKRASSDNGSGPSEKKPNLEAKTPTTLIEPIRLGGISSTEEMDMKVLQFKNKKLAE-RLEQRQGFEDELREKIEKLEKRQATDDATLLIVNRYWSKVESAKETAMETDETSPEPPPAPPFSTGALPESSLTFLATLAHCTKEEIELKLQERMEFSKKAVSCVLETSNKLHCRTEELCDR---------------------------------------IHKGDFDHLGESPKKLNKELITENRRLQDLATVLQEKHHRLSLQHHELQDRATSTETKVSEMERTIEDLQWDIEKLRKHEQKLNRHLAEALEQ 276
BLAST of E3 ubiquitin protein ligase vs. Ensembl Xenopus
Match: mphosph10 (M-phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein) [Source:Xenbase;Acc:XB-GENE-940335]) HSP 1 Score: 289.271 bits (739), Expect = 3.250e-83 Identity = 156/354 (44.07%), Postives = 243/354 (68.64%), Query Frame = 3
Query: 1746 EMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE----PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
+++K L++EL ++E KE+ LL+ Y++ K+QRDK QLMA+E + E +E+ +R+++ EK E D + LR+++ EE + Q+KL KQ+E+A++++ VT QA+ED+ EQN RL+Q L+EKD+ N KLM++R+K+NQ KLL+E+++ +Q++ K Q++A + K EE+ERL +L ++EKE T R Q+LEL+KRKA+E Q AEDL++ ++ L+++Q + + SA+ +E+F +R +E++ LRRK E+ KK+E + D++L EI+ Y+ LTC C + KKDA+LTKCFHVFC C+KTRYE R RKCPKCNA FGA+D+H IY+
Sbjct: 534 DILKQLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKTKAE---MEEQRTRVQALEEKDKRERKKLADEEALRKIKMAEEQIDHLQRKLTATKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLREEKEELAEQVQGLKAQVDAQLLQVQKLEERERLLHTSLTSIEKEVTLRTQSLELNKRKAVEAAQLAEDLKVQMEHVGETLRDTQNFVCENRSAKEKESFSLKRAQEDVSRLRRKLEKQKKMEVYADADQILQEEIKEYRARLTCPCCNTRKKDAVLTKCFHVFCFECVKTRYESRQRKCPKCNAAFGAHDFHRIYI 884 HSP 2 Score: 120.939 bits (302), Expect = 2.981e-27 Identity = 96/321 (29.91%), Postives = 153/321 (47.66%), Query Frame = 3
Query: 75 SKRPADFDETANDETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLEGDSCEVS--------------------ETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEK 974
SKR + + + E PN+ K + E ++ G I S EE+DMK LQ +NKKL+E L+Q+Q + +++IE+LE RQA DD + VNRYW+ LDED +LLKR + + + S E++ FL LA KE++E KL+ER+ FSK+ +S ++E K + R E L + I +G D + E+ K EL EN R+ L + L +HH L ++ E++D+ + K+ E + ED ++ + + + ++NR L E E+
Sbjct: 6 SKRASSDNGSGPSEKKPNLEAKTPTTLIEPIRLGGISSTEEMDMKVLQFKNKKLAE-RLEQRQGFEDELREKIEKLEKRQATDDATLLIVNRYWSKLDEDVHVLLKRYDSEPSKQSAAPTSPEPPPAPPFSTGALPESSLTFLATLAHCTKEEIELKLQERMEFSKKAVSCVLETSNKLHCRTEELCDR---------------------------------------IHKGDFDHLGESPKKLNKELITENRRLQDLATVLQEKHHRLSLQHHELQDRATSTETKVSEMERTIEDLQWDIEKLRKHEQKLNRHLAEALEQ 286
BLAST of E3 ubiquitin protein ligase vs. Ensembl Xenopus
Match: mphosph10 (M-phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein) [Source:Xenbase;Acc:XB-GENE-940335]) HSP 1 Score: 290.041 bits (741), Expect = 4.699e-83 Identity = 160/370 (43.24%), Postives = 249/370 (67.30%), Query Frame = 3
Query: 1713 PSTFDSLTSEE-----EMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE----PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
P+T + T E +++K L++EL ++E KE+ LL+ Y++ K+QRDK QLMA+E + E +E+ +R+++ EK E D + LR+++ EE + Q+KL KQ+E+A++++ VT QA+ED+ EQN RL+Q L+EKD+ N KLM++R+K+NQ KLL+E+++ +Q++ K Q++A + K EE+ERL +L ++EKE T R Q+LEL+KRKA+E Q AEDL++ ++ L+++Q + + SA+ +E+F +R +E++ LRRK E+ KK+E + D++L EI+ Y+ LTC C + KKDA+LTKCFHVFC C+KTRYE R RKCPKCNA FGA+D+H IY+
Sbjct: 589 PTTPEQKTDESKRKDCDILKQLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKTKAE---MEEQRTRVQALEEKDKRERKKLADEEALRKIKMAEEQIDHLQRKLTATKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLREEKEELAEQVQGLKAQVDAQLLQVQKLEERERLLHTSLTSIEKEVTLRTQSLELNKRKAVEAAQLAEDLKVQMEHVGETLRDTQNFVCENRSAKEKESFSLKRAQEDVSRLRRKLEKQKKMEVYADADQILQEEIKEYRARLTCPCCNTRKKDAVLTKCFHVFCFECVKTRYESRQRKCPKCNAAFGAHDFHRIYI 955 HSP 2 Score: 209.534 bits (532), Expect = 2.408e-55 Identity = 162/553 (29.29%), Postives = 276/553 (49.91%), Query Frame = 3
Query: 75 SKRPADFDETANDETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLEGD---------------------SCEVSETAE------------------YFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQA 1613
SKR + + + E PN+ K + E ++ G I S EE+DMK LQ +NKKL+E L+Q+Q + +++IE+LE RQA DD + VNRYW+ LDED +LLKR + + + E E+AE FL LA KE++E KL+ER+ FSK+ +S ++E K + R E L + I +G D + E+ K EL EN R+ L + L +HH L ++ E++D+ + K+ E + ED ++ + + + ++NR L E AL+ + G + ++ K + L EL+ +E R++ELEKL+ + Q + +LKL ++ +PE + ++ ++ L +QF++LY E++ ++ QL+EARA + + + HL+QIE ME E +Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K + QR ++K ++ QA
Sbjct: 6 SKRASSDNGSGPSEKKPNLEAKTPTTLIEPIRLGGISSTEEMDMKVLQFKNKKLAE-RLEQRQGFEDELREKIEKLEKRQATDDATLLIVNRYWSKLDEDVHVLLKRYDSEPSKQSAAPSAATEEPESAKETAMETDESAEGSQPPPTQEIKALPESSLTFLATLAHCTKEEIELKLQERMEFSKKAVSCVLETSNKLHCRTEELCDR---------------------------------------IHKGDFDHLGESPKKLNKELITENRRLQDLATVLQEKHHRLSLQHHELQDRATSTETKVSEMERTIEDLQWDIEKLRKHEQKLNRHLAE------ALEQLNSGYHISGSSGSFQGGQTTLSMQKFEMLNAELDQNQELANSRISELEKLQNELQAAVRLKEKLKLDLRTLPEEAVRNTQDFRCLQSQFSLLYNESLQVKTQLDEARALLLSTKNSHLRQIERMESEELSVQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLIGSLQNHNHQLKGDVQRYKRKVRDSQA 512
BLAST of E3 ubiquitin protein ligase vs. Ensembl Xenopus
Match: mphosph10 (M-phase phosphoprotein 10 (U3 small nucleolar ribonucleoprotein) [Source:Xenbase;Acc:XB-GENE-940335]) HSP 1 Score: 289.656 bits (740), Expect = 6.804e-83 Identity = 156/354 (44.07%), Postives = 243/354 (68.64%), Query Frame = 3
Query: 1746 EMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE----PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
+++K L++EL ++E KE+ LL+ Y++ K+QRDK QLMA+E + E +E+ +R+++ EK E D + LR+++ EE + Q+KL KQ+E+A++++ VT QA+ED+ EQN RL+Q L+EKD+ N KLM++R+K+NQ KLL+E+++ +Q++ K Q++A + K EE+ERL +L ++EKE T R Q+LEL+KRKA+E Q AEDL++ ++ L+++Q + + SA+ +E+F +R +E++ LRRK E+ KK+E + D++L EI+ Y+ LTC C + KKDA+LTKCFHVFC C+KTRYE R RKCPKCNA FGA+D+H IY+
Sbjct: 612 DILKQLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKTKAE---MEEQRTRVQALEEKDKRERKKLADEEALRKIKMAEEQIDHLQRKLTATKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLREEKEELAEQVQGLKAQVDAQLLQVQKLEERERLLHTSLTSIEKEVTLRTQSLELNKRKAVEAAQLAEDLKVQMEHVGETLRDTQNFVCENRSAKEKESFSLKRAQEDVSRLRRKLEKQKKMEVYADADQILQEEIKEYRARLTCPCCNTRKKDAVLTKCFHVFCFECVKTRYESRQRKCPKCNAAFGAHDFHRIYI 962 HSP 2 Score: 209.149 bits (531), Expect = 3.654e-55 Identity = 163/561 (29.06%), Postives = 277/561 (49.38%), Query Frame = 3
Query: 75 SKRPADFDETANDETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLEGDSCEVS---------------------------------------ETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMN-ELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKM 1634
SKR + + + E PN+ K + E ++ G I S EE+DMK LQ +NKKL+E L+Q+Q + +++IE+LE RQA DD + VNRYW+ LDED +LLKR + + + S E++ FL LA KE++E KL+ER+ FSK+ +S ++E K + R E L + I +G D + E+ K EL EN R+ L + L +HH L ++ E++D+ + K+ E + ED ++ + + + ++NR L E E+ + EI K E++N EL+ +E R++ELEKL+ + Q + +LKL ++ +PE + ++ ++ L +QF++LY E++ ++ QL+EARA + + + HL+QIE ME E +Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K + QR ++K ++ QA KL+
Sbjct: 6 SKRASSDNGSGPSEKKPNLEAKTPTTLIEPIRLGGISSTEEMDMKVLQFKNKKLAE-RLEQRQGFEDELREKIEKLEKRQATDDATLLIVNRYWSKLDEDVHVLLKRYDSEPSKQSAAPSAATEEPESAKETAMETDETSPEPPPAPPFSTGALPESSLTFLATLAHCTKEEIELKLQERMEFSKKAVSCVLETSNKLHCRTEELCDR---------------------------------------IHKGDFDHLGESPKKLNKELITENRRLQDLATVLQEKHHRLSLQHHELQDRATSTETKVSEMERTIEDLQWDIEKLRKHEQKLNRHLAEALEQ---VGMEIY-----------------PNSMKSFEMLNAELDQNQELANSRISELEKLQNELQAAVRLKEKLKLDLRTLPEEAVRNTQDFRCLQSQFSLLYNESLQVKTQLDEARALLLSTKNSHLRQIERMESEELSVQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLIGSLQNHNHQLKGDVQRYKRKVRDSQAEVSKLRF 506
BLAST of E3 ubiquitin protein ligase vs. Ensembl Mouse
Match: Rnf40 (ring finger protein 40 [Source:MGI Symbol;Acc:MGI:2142048]) HSP 1 Score: 304.679 bits (779), Expect = 7.013e-89 Identity = 163/361 (45.15%), Postives = 240/361 (66.48%), Query Frame = 3
Query: 1740 EEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE--------PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYLT 2798
E E++K L++EL ++E KE+ LL+ Y++ K+QRDK QLMA+E K EV L+S + + D D LRR+R+ EE ++ Q+KL KQ+E+A++++ VT QA+ED+ EQN RLLQ L+EKD+ N KLM++R+KANQ KLL+E++ +Q+ K+Q++A ++ K EEKER +L +EKE T R QALEL+KRKA+E Q AEDL++ L+ ++L+E Q L + +A+ +E+F +R +E++ LRRK E+ +K+E + DE+L EI+ YK LTC C + KKDA+LTKCFHVFC C++ RYE R RKCPKCNA FGA+D+H +Y++
Sbjct: 548 ESELLKGLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKA-------KAEVDELRSRIRELEERDRRESKKIADEDALRRIRQAEEQIEHLQRKLGATKQEEEALLSEMDVTGQAFEDMQEQNGRLLQQLREKDDANFKLMSERIKANQIHKLLREEKDELGEQVLGLKSQVDAQLLTVQKLEEKERALQGSLGGVEKELTLRSQALELNKRKAVEAAQLAEDLKVQLEHVQTRLREIQPCLAESRAAREKESFNLKRAQEDISRLRRKLEKQRKVEVYADADEILQEEIKEYKARLTCPCCNTRKKDAVLTKCFHVFCFECVRGRYEARQRKCPKCNAAFGAHDFHRVYIS 901 HSP 2 Score: 97.8265 bits (242), Expect = 3.279e-20 Identity = 91/342 (26.61%), Postives = 151/342 (44.15%), Query Frame = 3
Query: 69 LMSKRPADFDETANDETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLK----------RLEGDSCE-----------------------------VSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEK 974
L +KR A + E N K + E ++ G I S EE+D K LQ +NKKL+E L+Q+Q + ++RIE+LE RQA DD + VNRYW LDE + LL+ E C+ +SE A F+ L + E++E +L+ R+ FSK +SR+VEA ++ ++ E L ++V S +G + E ++ EL +EN R+ L ++L +HH + E E++DK+ + K+ E ++ ED ++ + + + ++N+ L E E+
Sbjct: 4 LSNKRAAGDGGSGPPEKKMNREEKTTTTLIEPIRLGGISSTEEMDSKVLQFKNKKLAE-RLEQRQACEDELRERIEKLEKRQATDDATLLIVNRYWAQLDETVEALLQCYENQRELSSGTEVPGCQEGLTRDVIPRPDPGTSDLREPLPVQFRAPLSEPALAFVVALGASSCEEVELQLQGRMEFSKAAVSRVVEASDRLQRQVEEL------------------------CQRVYS--------------RGDSEAPGEVARVRTRELGRENRRLQDLATQLQEKHHRISLEYSELQDKVTSTETKVLEMETTVEDLQWDIEKLRKREQKLNKHLAEALEQ 306 HSP 3 Score: 60.8474 bits (146), Expect = 8.963e-9 Identity = 29/88 (32.95%), Postives = 56/88 (63.64%), Query Frame = 3
Query: 1383 IQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMDNTN 1646
+Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K +AQR ++K +E+QA KL+ +
Sbjct: 337 LQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLISSLQNHNHQLKGDAQRYKRKLREVQAEIGKLRAQASG 424
BLAST of E3 ubiquitin protein ligase vs. Ensembl Mouse
Match: Rnf40 (ring finger protein 40 [Source:MGI Symbol;Acc:MGI:2142048]) HSP 1 Score: 304.294 bits (778), Expect = 2.679e-88 Identity = 163/361 (45.15%), Postives = 240/361 (66.48%), Query Frame = 3
Query: 1740 EEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE--------PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYLT 2798
E E++K L++EL ++E KE+ LL+ Y++ K+QRDK QLMA+E K EV L+S + + D D LRR+R+ EE ++ Q+KL KQ+E+A++++ VT QA+ED+ EQN RLLQ L+EKD+ N KLM++R+KANQ KLL+E++ +Q+ K+Q++A ++ K EEKER +L +EKE T R QALEL+KRKA+E Q AEDL++ L+ ++L+E Q L + +A+ +E+F +R +E++ LRRK E+ +K+E + DE+L EI+ YK LTC C + KKDA+LTKCFHVFC C++ RYE R RKCPKCNA FGA+D+H +Y++
Sbjct: 608 ESELLKGLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKA-------KAEVDELRSRIRELEERDRRESKKIADEDALRRIRQAEEQIEHLQRKLGATKQEEEALLSEMDVTGQAFEDMQEQNGRLLQQLREKDDANFKLMSERIKANQIHKLLREEKDELGEQVLGLKSQVDAQLLTVQKLEEKERALQGSLGGVEKELTLRSQALELNKRKAVEAAQLAEDLKVQLEHVQTRLREIQPCLAESRAAREKESFNLKRAQEDISRLRRKLEKQRKVEVYADADEILQEEIKEYKARLTCPCCNTRKKDAVLTKCFHVFCFECVRGRYEARQRKCPKCNAAFGAHDFHRVYIS 961 HSP 2 Score: 111.309 bits (277), Expect = 2.227e-24 Identity = 53/154 (34.42%), Postives = 101/154 (65.58%), Query Frame = 3
Query: 1185 KLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMDNTN 1646
K+ ++ +PE V+ ++ +Y+ L AQF++LY E++ ++ QL+EAR + ++ HL+ IE ME +E +Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K +AQR ++K +E+QA KL+ +
Sbjct: 331 KVALRSLPEEVVRETGEYRMLQAQFSLLYNESLQVKTQLDEARGLLLASKNSHLRHIEHMESDELGLQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLISSLQNHNHQLKGDAQRYKRKLREVQAEIGKLRAQASG 484 HSP 3 Score: 97.8265 bits (242), Expect = 3.225e-20 Identity = 91/342 (26.61%), Postives = 151/342 (44.15%), Query Frame = 3
Query: 69 LMSKRPADFDETANDETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLK----------RLEGDSCE-----------------------------VSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEK 974
L +KR A + E N K + E ++ G I S EE+D K LQ +NKKL+E L+Q+Q + ++RIE+LE RQA DD + VNRYW LDE + LL+ E C+ +SE A F+ L + E++E +L+ R+ FSK +SR+VEA ++ ++ E L ++V S +G + E ++ EL +EN R+ L ++L +HH + E E++DK+ + K+ E ++ ED ++ + + + ++N+ L E E+
Sbjct: 4 LSNKRAAGDGGSGPPEKKMNREEKTTTTLIEPIRLGGISSTEEMDSKVLQFKNKKLAE-RLEQRQACEDELRERIEKLEKRQATDDATLLIVNRYWAQLDETVEALLQCYENQRELSSGTEVPGCQEGLTRDVIPRPDPGTSDLREPLPVQFRAPLSEPALAFVVALGASSCEEVELQLQGRMEFSKAAVSRVVEASDRLQRQVEEL------------------------CQRVYS--------------RGDSEAPGEVARVRTRELGRENRRLQDLATQLQEKHHRISLEYSELQDKVTSTETKVLEMETTVEDLQWDIEKLRKREQKLNKHLAEALEQ 306
BLAST of E3 ubiquitin protein ligase vs. Ensembl Mouse
Match: Rnf40 (ring finger protein 40 [Source:MGI Symbol;Acc:MGI:2142048]) HSP 1 Score: 304.679 bits (779), Expect = 4.545e-88 Identity = 163/361 (45.15%), Postives = 240/361 (66.48%), Query Frame = 3
Query: 1740 EEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE--------PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYLT 2798
E E++K L++EL ++E KE+ LL+ Y++ K+QRDK QLMA+E K EV L+S + + D D LRR+R+ EE ++ Q+KL KQ+E+A++++ VT QA+ED+ EQN RLLQ L+EKD+ N KLM++R+KANQ KLL+E++ +Q+ K+Q++A ++ K EEKER +L +EKE T R QALEL+KRKA+E Q AEDL++ L+ ++L+E Q L + +A+ +E+F +R +E++ LRRK E+ +K+E + DE+L EI+ YK LTC C + KKDA+LTKCFHVFC C++ RYE R RKCPKCNA FGA+D+H +Y++
Sbjct: 648 ESELLKGLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKA-------KAEVDELRSRIRELEERDRRESKKIADEDALRRIRQAEEQIEHLQRKLGATKQEEEALLSEMDVTGQAFEDMQEQNGRLLQQLREKDDANFKLMSERIKANQIHKLLREEKDELGEQVLGLKSQVDAQLLTVQKLEEKERALQGSLGGVEKELTLRSQALELNKRKAVEAAQLAEDLKVQLEHVQTRLREIQPCLAESRAAREKESFNLKRAQEDISRLRRKLEKQRKVEVYADADEILQEEIKEYKARLTCPCCNTRKKDAVLTKCFHVFCFECVRGRYEARQRKCPKCNAAFGAHDFHRVYIS 1001 HSP 2 Score: 204.142 bits (518), Expect = 1.216e-53 Identity = 161/566 (28.45%), Postives = 279/566 (49.29%), Query Frame = 3
Query: 69 LMSKRPADFDETANDETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLK----------RLEGDSCE-----------------------------VSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMDNTN 1646
L +KR A + E N K + E ++ G I S EE+D K LQ +NKKL+E L+Q+Q + ++RIE+LE RQA DD + VNRYW LDE + LL+ E C+ +SE A F+ L + E++E +L+ R+ FSK +SR+VEA ++ ++ E L ++V S +G + E ++ EL +EN R+ L ++L +HH + E E++DK+ + K+ E ++ ED ++ + + + ++N+ L E AL+ + G ++ K + L ELE+ +E R+ ELEKL+ + Q + RLK+ ++ +PE V+ ++ +Y+ L AQF++LY E++ ++ QL+EAR + ++ HL+ IE ME +E +Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K +AQR ++K +E+QA KL+ +
Sbjct: 4 LSNKRAAGDGGSGPPEKKMNREEKTTTTLIEPIRLGGISSTEEMDSKVLQFKNKKLAE-RLEQRQACEDELRERIEKLEKRQATDDATLLIVNRYWAQLDETVEALLQCYENQRELSSGTEVPGCQEGLTRDVIPRPDPGTSDLREPLPVQFRAPLSEPALAFVVALGASSCEEVELQLQGRMEFSKAAVSRVVEASDRLQRQVEEL------------------------CQRVYS--------------RGDSEAPGEVARVRTRELGRENRRLQDLATQLQEKHHRISLEYSELQDKVTSTETKVLEMETTVEDLQWDIEKLRKREQKLNKHLAE------ALEQLNSGYYVSGSSTGFQGGQITLSMQKFEMLNAELEENQELANSRMAELEKLQAELQGAVRTNERLKVALRSLPEEVVRETGEYRMLQAQFSLLYNESLQVKTQLDEARGLLLASKNSHLRHIEHMESDELGLQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLISSLQNHNHQLKGDAQRYKRKLREVQAEIGKLRAQASG 524
BLAST of E3 ubiquitin protein ligase vs. Ensembl Mouse
Match: Rnf20 (ring finger protein 20 [Source:MGI Symbol;Acc:MGI:1925927]) HSP 1 Score: 286.574 bits (732), Expect = 8.698e-82 Identity = 163/358 (45.53%), Postives = 239/358 (66.76%), Query Frame = 3
Query: 1725 DSLTSEEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE-PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
D E E+IK L+ EL ++E KE+ LL+ Y++ K+QRDK QLMA+E + E ++ + L+ +K + D D LR++R +EE ++ Q+KLA+AKQ+E+A++++ VT QA+ED+ EQN RL+Q L+EKD+ N KLM++R+K+NQ KLLKE+++ DQ+ KTQ++A + + K EEKE L +N+ T EKE R QALE++KRKAME Q A+DL+ L+ +L + Q + + + + ++ F +R +E++ LRRK E KK ++ DE+L+ EI+ YK LTC C KKDA+LTKCFHVFC C+KTRY+ R RKCPKCNA FGAND+H IY+
Sbjct: 615 DGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDLFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYI 972
BLAST of E3 ubiquitin protein ligase vs. Ensembl Mouse
Match: Rnf20 (ring finger protein 20 [Source:MGI Symbol;Acc:MGI:1925927]) HSP 1 Score: 286.574 bits (732), Expect = 8.698e-82 Identity = 163/358 (45.53%), Postives = 239/358 (66.76%), Query Frame = 3
Query: 1725 DSLTSEEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE-PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
D E E+IK L+ EL ++E KE+ LL+ Y++ K+QRDK QLMA+E + E ++ + L+ +K + D D LR++R +EE ++ Q+KLA+AKQ+E+A++++ VT QA+ED+ EQN RL+Q L+EKD+ N KLM++R+K+NQ KLLKE+++ DQ+ KTQ++A + + K EEKE L +N+ T EKE R QALE++KRKAME Q A+DL+ L+ +L + Q + + + + ++ F +R +E++ LRRK E KK ++ DE+L+ EI+ YK LTC C KKDA+LTKCFHVFC C+KTRY+ R RKCPKCNA FGAND+H IY+
Sbjct: 615 DGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLELAQKKLHDFQDEIVENSVTKEKDLFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYI 972
BLAST of E3 ubiquitin protein ligase vs. UniProt/SwissProt
Match: sp|Q9VRP9|BRE1_DROME (E3 ubiquitin-protein ligase Bre1 OS=Drosophila melanogaster OX=7227 GN=Bre1 PE=1 SV=2) HSP 1 Score: 318.546 bits (815), Expect = 4.843e-92 Identity = 171/377 (45.36%), Postives = 255/377 (67.64%), Query Frame = 3
Query: 1740 EEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLK-SNLEKRLHEPDPDQLRRVRKMEETLKDYQQKLA-----------------------LAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNT-DEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
E E +++L+++L + KE+ LL+ Y+ +KDQRDK QLMA+E +R E ++++ +L+ S E+R D + LR+++++EE + Q+++A + +E+A++N+ VT QA+ED+ EQNSRL+Q L+EKD+ N KLM++R+KANQ KLL+E++ + EDQ+ TQIEA++ L K EEKER + ++EKE RQQA+E+HKRKA+E Q+A DL+++L+KY +Q+KE+Q + +K S+ EA+K +R++EEL +RK ER+KK+E S T DEV+I EI+ YK+ LTC +CK +KDA+L+KCFHVFC CL+TRYE R RKCPKCN FGANDYH +YL
Sbjct: 667 ESETVRDLKAQLKKALNDQKEMKLLLDMYKGVSKDQRDKVQLMATEKKLRSEIEELRQQLKKLQESKREERKKLADEEALRKIKQLEEQKYELQKQMANHKPTDNSWGSGAPGTANYTRPFVGSHEEEALLNEMEVTGQAFEDMQEQNSRLIQQLREKDDANFKLMSERIKANQLHKLLREEKTVLEDQMATATTQIEAMHIVLRKLEEKERSLQATVASIEKELMLRQQAMEMHKRKAIESAQSAADLKLHLEKYHAQMKEAQQVVAEKTSSLEAEAYKTKRLQEELAQFKRKAERMKKMEMSGTTIDEVMIEEIREYKETLTCPSCKVKRKDAVLSKCFHVFCYDCLRTRYETRQRKCPKCNCAFGANDYHRLYL 1043 HSP 2 Score: 221.09 bits (562), Expect = 3.157e-58 Identity = 167/546 (30.59%), Postives = 289/546 (52.93%), Query Frame = 3
Query: 72 MSKRPADFDETAND-----ETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNHKDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLEGDSCEV------SETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHEL--------------KSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKM 1634
MSKR AD D T + P+K+ +E G + + EE+D+K L+ QNKKL++ + + + + RIEQLE RQ +DD ++ VNRYWN L+ED ++LL+R + ++ + +E FL QL++WDKE+L+ KL RV SKR ++++V+ ++ +R E + +H +K + + + E ++ + V + E LK E+ EN ++ L + LH + H + ++EN E+K++++ LE+ + + E LAE ++ + N+ + K T +++ ++EL ELE+ +E RL EL+KL H+ L+EV +LK+ ++++PESVI+++ +YK L +QF++LY E++ ++ L+E R +QT++ HL+QIE ME E + Q ++RS+M+ M + + +R EYE L +E +Q + N+ + PI EMR LI +L+ Q K E QR ++KYK+ + D LK+
Sbjct: 1 MSKRSAD-DATGSSCLVAAAAAGQPPIKKV--HFEPHLIGPVSTLEEMDIKVLEFQNKKLAQRIEQRMRTEAELRHRIEQLEKRQTQDDAVLNVVNRYWNQLNEDIRVLLQRFDAETADELENRNENEVTTSFLAQLSTWDKEELDEKLANRVQVSKRAVAKIVQVIDRLMQRNEKI------THVLKGDSLASAGSGSGAGAGGEEEQQQASGDAETTTSSAGVHALEETLKQTHIEIMSENHKLQNLNTSLHEKFHTMSLKMKEYQDAHTAKETENAELKNQIDELQYDLEKIHCRNDKLENHLAEAIEKLKAYHQIYGDPNKSTNSAK--------TPTTTGSGGATTSVNSQLLEELQKELEEYRELANNRLQELDKLHATHRETLKEVEKLKMDIRQLPESVIVETTEYKCLQSQFSVLYNESMQIKTMLDETRNQLQTSKNQHLRQIEVMESEELIAQKKVRSEMIQMEDVLALIRKEYETLRIEFEQNMAANEQTAPINREMRHLITSLQNHNGQLKGEVQRYKRKYKD--TSTDNLKL 527
BLAST of E3 ubiquitin protein ligase vs. UniProt/SwissProt
Match: sp|Q8CJB9|BRE1B_RAT (E3 ubiquitin-protein ligase BRE1B OS=Rattus norvegicus OX=10116 GN=Rnf40 PE=1 SV=1) HSP 1 Score: 307.76 bits (787), Expect = 2.243e-88 Identity = 165/358 (46.09%), Postives = 244/358 (68.16%), Query Frame = 3
Query: 1740 EEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE-----PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYLT 2798
E E++K L++EL ++E KE+ LL+ Y++ K+QRDK QLMA+E + E E SR++ +LE+R D D LRR+R+ EE ++ Q+KL KQ+E+A++++ VT QA+ED+ EQN RLLQ L+EKD+ N KLM++R+KANQ KLL+E++ +Q+ K+Q++A ++ K EEKER +L +EKE T R QALEL+KRKA+E Q AEDL++ L+ ++L+E Q L + +A+ +E+F +R +E++ LRRK E+ +K+E + DE+L EI+ YK LTC C + KKDA+LTKCFHVFC C++ RYE R RKCPKCNA FGA+D+H +Y++
Sbjct: 649 ESELLKGLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKAKAE---VDELRSRIR-DLEERDRRESKKIADEDALRRIRQAEEQIEHLQRKLGATKQEEEALLSEMDVTGQAFEDMQEQNGRLLQQLREKDDANFKLMSERIKANQIHKLLREEKDELGEQVLGLKSQVDAQLLTVQKLEEKERALQGSLGGVEKELTLRSQALELNKRKAVEAAQLAEDLKVQLEHVQTRLREIQPCLAESRAAREKESFNLKRAQEDISRLRRKLEKQRKVEVYADADEILQEEIKEYKARLTCPCCNTRKKDAVLTKCFHVFCFECVRGRYEARQRKCPKCNAAFGAHDFHRVYIS 1002 HSP 2 Score: 203.371 bits (516), Expect = 1.727e-52 Identity = 162/567 (28.57%), Postives = 277/567 (48.85%), Query Frame = 3
Query: 69 LMSKRPADFDETANDETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILL------KRLEGDSCEV----------------------------------SETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMDNTN 1646
L +KR A + E N K + E ++ G I S EE+D K LQ +NKKL+E L+Q+Q + ++RIE+LE RQA DD + VNRYW LDE + LL + L EV SE A F+ L + E++E +L+ R+ FSK +SR+VEA ++ ++ E L ++V S +G + E + EL +EN R+ L ++L +HH + E E++DK+ + K+ E ++ ED ++ + + + ++N+ L E AL+ + G ++ K + L ELE+ +E R+ ELEKL+ + Q + RLK+ ++ +PE V+ ++ +Y+ L AQF++LY E++ ++ QL+EAR + ++ HL+ IE ME +E +Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K +AQR ++K +E+QA KL+ +
Sbjct: 4 LSNKRAAGDGGSGPPEKKLNREEKTTTTLIEPIRLGGISSTEEMDSKVLQFKNKKLAE-RLEQRQACEDELRERIEKLEKRQATDDATLLIVNRYWAQLDETVEALLQCYENQRELSSSGTEVPGCQEGLTRDVIPRTDPGTSDLREPLPMQFRAPLSEPALAFVVALGASSCEEVELQLQGRMEFSKAAVSRVVEASDRLQRQVEEL------------------------CQRVYS--------------RGDSEAPGEVARARTRELGRENRRLQDLATQLQEKHHRISLEYSELQDKVTSTETKVLEMETTVEDLQWDIEKLRKREQKLNKHLAE------ALEQLNSGYYVSGSSTGFQGGQITLSMQKFEMLNAELEENQELANSRMAELEKLQAELQGAVRTNERLKVALRSLPEEVVRETGEYRMLQAQFSLLYNESLQVKTQLDEARGLLLASKNSHLRHIEHMESDELGLQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLISSLQNHNHQLKGDAQRYKRKLREVQAEIGKLRAQASG 525
BLAST of E3 ubiquitin protein ligase vs. UniProt/SwissProt
Match: sp|Q4R7K7|BRE1B_MACFA (E3 ubiquitin-protein ligase BRE1B OS=Macaca fascicularis OX=9541 GN=RNF40 PE=2 SV=1) HSP 1 Score: 306.99 bits (785), Expect = 3.975e-88 Identity = 168/388 (43.30%), Postives = 246/388 (63.40%), Query Frame = 3
Query: 1701 GLSDPSTFDSLT--------------SEEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE--------PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYLT 2798
GL P +L+ E E++K L++EL ++E KE+ LL+ Y++ K+QRDK QLMA+E K EV L+S + + D D LRR+R+ EE ++ Q+KL KQ+E+A++++ VT QA+ED+ EQN RLLQ L+EKD+ N KLM++R+KANQ KLL+E++ +Q+ K+Q++A ++ K EEKER +L +EKE T R QALEL+KRKA+E Q AEDL++ L+ ++L+E Q L + +A+ +E+F +R +E++ LRRK E+ +K+E + DE+L EI+ YK LTC C + KKDA+LTKCFHVFC C++ RYE R RKCPKCNA FGA+D+H IY++
Sbjct: 621 GLGPPPVASALSRADREKAKVEEAKRKESELLKGLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKA-------KAEVDELRSRIRELEERDRRESKKIADGDALRRIRQAEEQIEHLQRKLGATKQEEEALLSEMDVTGQAFEDMQEQNGRLLQQLREKDDANFKLMSERIKANQIHKLLREEKDELGEQVLGLKSQVDAQLLTVQKLEEKERALQGSLGGVEKELTLRSQALELNKRKAVEAAQLAEDLKVQLEHVQTRLREIQPCLAESRAAREKESFNLKRAQEDISRLRRKLEKQRKVEVYADADEILQEEIKEYKARLTCPCCNTRKKDAVLTKCFHVFCFECVRGRYEARQRKCPKCNAAFGAHDFHRIYIS 1001 HSP 2 Score: 206.838 bits (525), Expect = 1.065e-53 Identity = 155/536 (28.92%), Postives = 271/536 (50.56%), Query Frame = 3
Query: 159 EQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLK----------------RLEGDSCE-----------------------VSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMDNTN 1646
E ++ G I S EE+D+K LQ +NKKL+E L+Q+Q + ++RIE+LE RQA DD + VNRYW LDE + LL+ EG +C+ +SE A F+ L + E++E +L+ R+ FSK +S +VEA ++ +R E L ++V S +G + ++E + EL +EN R+ L ++L +HH + E E++DK+ + K+ E ++ ED ++ + + + ++N+ L E AL+ + G ++ K + L ELE+ +E R+ ELEKL+ + Q + RLK+ ++ +PE V+ ++ +Y+ L AQF++LY E++ ++ QL+EAR + + HL+ IE ME +E +Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K +AQR ++K +E+QA KL+ +
Sbjct: 34 EPIRLGGISSTEEIDLKVLQFKNKKLAE-RLEQRQACEDELRERIEKLEKRQATDDATLLIVNRYWAQLDETVEALLRCHEGQGELSSAPEAPGTQEGPTCDGTPLPEPGTSELREPLPLQLRPPLSEPALAFVVALGASSSEEVELELQGRMEFSKAAVSHVVEASDRLQRRVEEL------------------------CQRVYS--------------RGDSEPLSEVARARTRELGRENRRLQDLATQLQEKHHRISLEYSELQDKVTSAETKVLEMETTVEDLQWDIEKLRKREQKLNKHLAE------ALEQLNSGYYVSGSSSGFQGGQITLSMQKFEMLNAELEENQELANSRMAELEKLQAELQGAVRTNERLKVALRSLPEEVVRETGEYRMLQAQFSLLYNESLQVKTQLDEARGLLLATKNSHLRHIEHMESDELGLQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLISSLQNHNHQLKGDAQRYKRKLREVQAEIGKLRAQTSG 524
BLAST of E3 ubiquitin protein ligase vs. UniProt/SwissProt
Match: sp|Q5RAU7|BRE1B_PONAB (E3 ubiquitin-protein ligase BRE1B OS=Pongo abelii OX=9601 GN=RNF40 PE=2 SV=1) HSP 1 Score: 305.449 bits (781), Expect = 1.312e-87 Identity = 164/361 (45.43%), Postives = 240/361 (66.48%), Query Frame = 3
Query: 1740 EEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE--------PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYLT 2798
E E++K L++EL ++E KE+ LL+ Y++ K+QRDK QLMA+E K EV L+S + + D D LRR+R+ EE ++ Q+KL KQ+E+A++++ VT QA+ED+ EQN RLLQ L+EKD+ N KLM++R+KANQ KLL+E++ +Q+ K+Q++A ++ K EEKER +L +EKE T R QALEL+KRKA+E Q AEDL++ L+ ++L+E Q L + +A+ +E+F +R +E++ LRRK E+ +K+E + DE+L EI+ YK LTC C + KKDA+LTKCFHVFC C++ RYE R RKCPKCNA FGA+D+H IY++
Sbjct: 648 ESELLKGLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKA-------KAEVDELRSRIRELEERDRRESKKIADEDALRRIRQAEEQIEHLQRKLGATKQEEEALLSEMDVTGQAFEDMQEQNGRLLQQLREKDDANFKLMSERIKANQIHKLLREEKDELGEQVLGLKSQVDAQLLTVQKLEEKERALQGSLGGVEKELTLRSQALELNKRKAVEAAQLAEDLKVQLEHVQTRLREIQPCLAESRAAREKESFNLKRAQEDISRLRRKLEKQRKVEVYADADEILQEEIKEYKARLTCPCCNTRKKDAVLTKCFHVFCFECVRGRYEARQRKCPKCNAAFGAHDFHRIYIS 1001 HSP 2 Score: 207.608 bits (527), Expect = 7.481e-54 Identity = 155/530 (29.25%), Postives = 269/530 (50.75%), Query Frame = 3
Query: 159 EQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLK----------------RLEGDSCE-----------------------VSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKL 1628
E ++ G I S EE+D+K LQ +NKKL+E L+Q+Q + ++RIE+LE RQA DD + VNRYW LDE + LL+ EG +C+ +SE A F+ L + E++E +L+ R+ FSK +SR+VEA ++ +R E L ++V S +G + ++E + EL +EN R+ L ++L +HH + E E++DK+ + K+ E ++ ED ++ + + + ++N+ L E AL+ + G ++ K + L ELE+ +E R+ ELEKL+ + Q + R K+ ++ +PE V+ ++ +Y+ L AQF++LY E++ ++ QL+EAR + + HL+ IE ME +E +Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K +AQR ++K +E+QA KL
Sbjct: 34 EPIRLGGISSTEEMDLKVLQFKNKKLAE-RLEQRQACEDELRERIEKLEKRQATDDATLLIVNRYWAQLDETVEALLRCHESQGELSSAPEAPGTQEGPTCDGTPLPEPGTSELRDPLPMQLRPPLSEPALAFVVALGASSSEEVELELQGRMEFSKAAVSRVVEASDRLQRRVEEL------------------------CQRVYS--------------RGDSEPLSEAARARTRELGRENRRLQDLATQLQEKHHRISLEYSELQDKVTSAETKVLEMETTVEDLQWDIEKLRKREQKLNKHLAE------ALEQLNSGYYVSGSSSGFQGGQITLSMQKFEMLNAELEENQELANSRMAELEKLQAELQGAVRTNERPKVALRSLPEEVVRETGEYRMLQAQFSLLYNESLQVKTQLDEARGLLLATKNSHLRHIEHMESDELGLQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLISSLQNHNHQLKGDAQRYKRKLREVQAEIGKL 518
BLAST of E3 ubiquitin protein ligase vs. UniProt/SwissProt
Match: sp|O75150|BRE1B_HUMAN (E3 ubiquitin-protein ligase BRE1B OS=Homo sapiens OX=9606 GN=RNF40 PE=1 SV=5) HSP 1 Score: 305.449 bits (781), Expect = 1.508e-87 Identity = 164/361 (45.43%), Postives = 240/361 (66.48%), Query Frame = 3
Query: 1740 EEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE--------PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYLT 2798
E E++K L++EL ++E KE+ LL+ Y++ K+QRDK QLMA+E K EV L+S + + D D LRR+R+ EE ++ Q+KL KQ+E+A++++ VT QA+ED+ EQN RLLQ L+EKD+ N KLM++R+KANQ KLL+E++ +Q+ K+Q++A ++ K EEKER +L +EKE T R QALEL+KRKA+E Q AEDL++ L+ ++L+E Q L + +A+ +E+F +R +E++ LRRK E+ +K+E + DE+L EI+ YK LTC C + KKDA+LTKCFHVFC C++ RYE R RKCPKCNA FGA+D+H IY++
Sbjct: 648 ESELLKGLRAELKKAQESQKEMKLLLDMYKSAPKEQRDKVQLMAAERKA-------KAEVDELRSRIRELEERDRRESKKIADEDALRRIRQAEEQIEHLQRKLGATKQEEEALLSEMDVTGQAFEDMQEQNGRLLQQLREKDDANFKLMSERIKANQIHKLLREEKDELGEQVLGLKSQVDAQLLTVQKLEEKERALQGSLGGVEKELTLRSQALELNKRKAVEAAQLAEDLKVQLEHVQTRLREIQPCLAESRAAREKESFNLKRAQEDISRLRRKLEKQRKVEVYADADEILQEEIKEYKARLTCPCCNTRKKDAVLTKCFHVFCFECVRGRYEARQRKCPKCNAAFGAHDFHRIYIS 1001 HSP 2 Score: 210.69 bits (535), Expect = 7.679e-55 Identity = 156/536 (29.10%), Postives = 272/536 (50.75%), Query Frame = 3
Query: 159 EQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLK----------------RLEGDSCE-----------------------VSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMDNTN 1646
E ++ G I S EE+D+K LQ +NKKL+E L+Q+Q + ++RIE+LE RQA DD + VNRYW LDE + LL+ EG +C+ +SE A F+ L + E++E +L+ R+ FSK +SR+VEA ++ +R E L ++V S +G + ++E + EL +EN R+ L ++L +HH + E E++DK+ + K+ E ++ ED ++ + + + ++N+ L E AL+ + G ++ K + L ELE+ +E R+ ELEKL+ + Q + RLK+ ++ +PE V+ ++ +Y+ L AQF++LY E++ ++ QL+EAR + + HL+ IE ME +E +Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K +AQR ++K +E+QA KL+ +
Sbjct: 34 EPIRLGGISSTEEMDLKVLQFKNKKLAE-RLEQRQACEDELRERIEKLEKRQATDDATLLIVNRYWAQLDETVEALLRCHESQGELSSAPEAPGTQEGPTCDGTPLPEPGTSELRDPLLMQLRPPLSEPALAFVVALGASSSEEVELELQGRMEFSKAAVSRVVEASDRLQRRVEEL------------------------CQRVYS--------------RGDSEPLSEAAQAHTRELGRENRRLQDLATQLQEKHHRISLEYSELQDKVTSAETKVLEMETTVEDLQWDIEKLRKREQKLNKHLAE------ALEQLNSGYYVSGSSSGFQGGQITLSMQKFEMLNAELEENQELANSRMAELEKLQAELQGAVRTNERLKVALRSLPEEVVRETGEYRMLQAQFSLLYNESLQVKTQLDEARGLLLATKNSHLRHIEHMESDELGLQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLISSLQNHNHQLKGDAQRYKRKLREVQAEIGKLRAQASG 524
BLAST of E3 ubiquitin protein ligase vs. TrEMBL
Match: A0A1S3GZF2 (E3 ubiquitin protein ligase OS=Lingula unguis OX=7574 GN=LOC106150393 PE=3 SV=1) HSP 1 Score: 617.846 bits (1592), Expect = 0.000e+0 Identity = 379/951 (39.85%), Postives = 588/951 (61.83%), Query Frame = 3
Query: 69 LMSKRPADFDETANDETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNHKDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLEGDSCE------VSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFE-KVESNE-------KSDNNLESLDI---PQGIVD--------------LINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMDNTNLXX---------XXXXXXXXXXRGVLLGLSDPSTFDSLTSEEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLK--SNLEKRLHEPDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
+ +KRP E+ T P P K+ + +E +K G I S EELD+KT+Q QNKKLS+ L +++ + + RIEQLE RQ DD ++C VNRYWN LDED ++LL+R + ++ + SE FL QL+SWDKE+LE L +RV FSKR I +L++A+++ +R E L + +KIEK + ++K E K E+ E KS E+ D +G V+ +++E +K EL +L KEN R+ L ++L HH ++ ++++K ++ E K+K +D EY L T R L E +K LK + ++ + +G S KSK +EL +ELE+ +E + RL ELE L+K HQ ++E RLK++++E+PESVI+++ +YKSL +QF++LY E + ++ QL+E+R +Q+++ HL+QIE+ME +E Q ++R++M+ + + +++R EYE L +E +QT+ N+ + PI EMR+LI +L+ +Q K E R ++K KE Q K K + ++ ++ +E + + S+ E+IK+L+ +L +++ KE+ LL+ Y++ K+QRDK QLMA E + + K + +++ EKR D + +R+++++EE++ D Q+ LA KQ+E+A++N+ VT QA+ED+ EQN RL+Q L+EKD+ N KLM++R+K+NQ KLLKE++ + +Q+ Q+E N + + EEKER+ NNL T+EKE + RQQALE+HKRKA+E QTA DLR++L+KY +QLK++Q ++ +K A +E+FK+RRI+E++ ILRRK ER KK+E + D VL+ E++ YK+ LTC +CK +KDA+LTKCFHVFCL CLKTRYE R RKCPKCNA FGAND+H IYL
Sbjct: 1 MSAKRP--LSESPTGSTGP--PSKKALVQFEPIKIGPIYSLEELDLKTVQFQNKKLSQRLEQRRRAEEDLRKRIEQLEKRQTTDDAVLCIVNRYWNQLDEDVRVLLERFDAETADEDERENQSEAVTSFLTQLSSWDKEELEQNLSQRVEFSKRAIGKLIQAYDRLLQRNEKLYHSI---KDLKIEKDGDAQKADMKEELKTETTERAGEDDSKSSAQKETEDQEGEKEGSVEKQDEEKPEKKNGEQILSEAVKTELLDLQKENKRLQNLVTKLQEIHHVTTLKHSDLQEKFTAAETEIAELKNKIDDLEYDLGNTTHSAQRFEFQLYEAAQK---LKSQTEVVTVEGEGGGGVEGVS---KSKFQELTSELEEQRELASTRLQELENLQKNHQDTVKEEERLKMELKELPESVIVETSEYKSLQSQFSVLYNECMQIRTQLDESRNLLQSSKNAHLRQIEQMESDELACQKKLRTEMIQLEDTLAQVRKEYEMLRIEFEQTLAANEQTGPINREMRNLITSLQNHNHQLKGEVARYKRKLKEAQTEIAKFKAEASSADQPLEEPPKEPSPPRQEEPIIIKKEEEEKMEKEREKGKSDSEIIKDLKQQLKKAQDSQKEMKLLLDMYKSAPKEQRDKVQLMAGEKKAKQDIDELKNHIRKMQEAERKEKRKLAED-EAIRKIKRLEESIADLQRNLAAQKQEEEALLNEMEVTGQAFEDMQEQNIRLIQQLREKDDANFKLMSERIKSNQIHKLLKEEKDVLMEQVTTLHQQVETQNLVVRRLEEKERILQNNLATVEKELSLRQQALEMHKRKAVESSQTAADLRLHLEKYQNQLKDAQITVAEKTGAMEQESFKYRRIQEDVAILRRKLERHKKIELAGTADAVLMEELKEYKEQLTCPSCKVKRKDAVLTKCFHVFCLECLKTRYETRQRKCPKCNAGFGANDFHRIYL 937
BLAST of E3 ubiquitin protein ligase vs. TrEMBL
Match: K1QQJ7 (E3 ubiquitin protein ligase OS=Crassostrea gigas OX=29159 GN=CGI_10013943 PE=3 SV=1) HSP 1 Score: 605.905 bits (1561), Expect = 0.000e+0 Identity = 388/1013 (38.30%), Postives = 591/1013 (58.34%), Query Frame = 3
Query: 69 LMSKRPADFDETANDETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNHKDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLEGDSCEVSET------AEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVES----------HGVKIEKSTNET---VDNVKFEKVESNEKSDNNLESLDI---P------------------------------------------QGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMDNTNLXXXXXXXXXXXXRGVLLGL----------------SDPS-------TFDSLTSEEE---------------MIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE-PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYLT 2798
+ SKRPA+ D+ + P P K+ ++ +E V+ G I + EELDMK L+ QNKKL+E + +K+ + K RI+QLE+RQ DD ++ VNRYWN LD+D ++LL+R + ++ + ET + FL L++WDK++LE KL +RV FSKR I +L++AF++ +R E L + E I+K ++ VD K + E+ EKS E +I P QGIV K EL+EL +EN R+ + LH +HHE E E++DK+ ++ E K+K +D EY ++ ++++R L + +K K+ +D V+ + G + +AKSK E+M ELE+ KE RLTELEKL K HQ L E+ +LK +Q +PE +I+DS +YK L +QF++LY +++ ++ QL++ R +QT++ HL+ +E++E +E Q ++RS+ + + + +++R EYE L +E ++T+ N+ + PI EMRSLI +L+ Q K EA RSR++ +E+QA +K+K + E++ +T+ S PS T L EEE +IK L+++L S+ KEL LL+ Y++ K+QRDK QLMA E R E K ++ R++ N K + + D ++++ KMEE + + + LA KQ+E+A++++ VT QA+ED+ EQN RLLQ LKEKD+ N KLM++R+KANQ KLL+E++++ DQ+ ++Q+EA N + K EEKE++ N + T+EKE QQA+E+HKRKA+E QTA DL+++L KY +QLKE+Q S+ +K A +E+FK++R++EE+ L+RK ER KK+E + DEVL+ EI YK+ LTC +CK NKKDA+LTKCFHVFCL CLKTRYE R RKCPKCN FGANDYH +Y++
Sbjct: 8 MSSKRPAE-DQGGGSSSGP--PAKKLLTRFEPVRIGTIGNLEELDMKVLRFQNKKLAERVEQRKRAEDELKRRIDQLENRQRTDDAVLMIVNRYWNQLDDDIRLLLQRFDAETADEIETKNESDETKAFLTLLSTWDKQELEQKLGQRVEFSKRAIGKLLQAFDRLLQRNEKLNKGIQEKVNQEVEGLNLSATSIKKEPSDVKMEVDEKKETEEEAGEKSAKTEEDKEIKTEPEEKTDETEKTEVKKEEETSETETKVSVKEEEPSEEKPEILSLQGIV-------KEELAELQRENRRLHNTATYLHQRHHEHTLEVAELQDKLTAAETEIAELKNKMDDLEYYHGCASQQVDKLDRHLNDALQKLKSFEDTTVV------HDGSGKGVTGVAKSKFDEMMYELEEQKELATNRLTELEKLSKDHQEALTEIEKLKTDLQHLPEHIIVDSTEYKCLQSQFSVLYNDSMQIRTQLDDTRNLLQTSKNTHLRHLEQLECDELETQKKLRSECLQLEDNLAQVRREYEMLRIEFERTLAQNEQTAPINHEMRSLIQSLQKHNQQLKAEASRSRRRAREIQADMNKMKAEGFKPEEKPEQTENPAGGSDQGESGGSGGGGEPGTSTPTQSAPSPPHPGMKTEGDLGKEEEKEEFDYREKGKTDMEVIKELRAQLKQSQASQKELKLLLDMYKSAPKEQRDKVQLMACEKKFRQEIDELKAQIKRMQENERKEKRKLAEEDAIKKISKMEEKISELSKNLATQKQREEALLSEMEVTGQAFEDMQEQNMRLLQQLKEKDDANFKLMSERIKANQIQKLLREEKEVLADQVATLQSQVEAQNLVVRKLEEKEQILQNTVTTMEKELGLTQQAMEMHKRKAVESSQTAADLKLHLDKYQAQLKEAQVSVAEKTGALEQESFKYKRMQEEIAKLQRKLERSKKIEMAGAADEVLMAEIAEYKEQLTCPSCKVNKKDAVLTKCFHVFCLECLKTRYETRQRKCPKCNGGFGANDYHRLYIS 1004
BLAST of E3 ubiquitin protein ligase vs. TrEMBL
Match: A0A4Y2D906 (E3 ubiquitin protein ligase OS=Araneus ventricosus OX=182803 GN=Bre1_1 PE=3 SV=1) HSP 1 Score: 566.229 bits (1458), Expect = 0.000e+0 Identity = 348/946 (36.79%), Postives = 564/946 (59.62%), Query Frame = 3
Query: 69 LMSKRPADFDETANDETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNHKDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLEGDSCEV------SETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLD--IPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKA--LKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLK----MDNT--NLXXXXXXXXXXXXRGVLLGLS------------DPSTFDSLT-------SEEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSR-LKSNLEKRLHEPDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYLT 2798
+ +KRPA D ++ + + + +E + G + + EE+DMK LQ QNKKL+E L ++++ + + RIEQLE RQ D+ ++C VNRYWN L+ED +ILL+R + ++ + +E FL L WD+++LE KL +RV S R ++++++AF++ +R + + V+ + S+ + SLD + Q V+L NEN L L L + LH +HH++ + E+ DK+ + E ++ ED EY L++T + +++ L+E K KA L D + S + ++K+++L ELE KE RL ELE L +H+ L+ V +LK+ +Q +PESV++++ +YK L +QF++LY E++ ++ LEE R + T++ HL+QIE+ME E +Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K E QR ++K KE+ A KL+ M T N+++E + + + + D D T S+ E+I+ L+++L S+E KEL LL+ Y+ K+QRDKAQLMA+E R E + ++ + L + ++R D + L++++ +E+ L+ Q++LA KQ+E+A++N+ +T QA+ED+ EQN RL+Q L+EKD+ N KLM++R+K+NQ KLL+E++ + +Q+ +K Q++A N + K EEKERL N+ T+EKE + RQQ+ E+ KRK++E Q++ DLR++L+KYLS LKE Q ++ +K SA +EAFK +R++E+++ LRR+ ER KK E ++ DEVL+ EI+ YK+ LTC +CK +KDA+LTKCFHVFC CLKTRYE R RKCPKCNA FGAND+H +YL
Sbjct: 1 MATKRPAPDDGSSKNSSNKRV-------HFEPCRIGPVSTQEEMDMKVLQFQNKKLAERLEQRQRQEADLRQRIEQLEKRQTSDEAVLCVVNRYWNQLNEDLRILLQRFDAETSDEYENRNENEATTSFLAMLGQWDRDELEEKLAQRVQVSTRAVAKVLQAFDRIVQRNQKI------------------------MLAVKGDPSSEEEIPSLDDAVRQANVELQNENKNLHL------------LVTGLHEKHHQMNLKFAEVNDKLAAQEIANAELRNHIEDLEYDLSKTKQREEKLDMHLSECLTKLKAYNLTD-----------SEGKPPVSTVTQAKMEDLQKELEAQKELANNRLMELETLNTQHKEALKLVEKLKMDLQCLPESVVVETTEYKCLQSQFSVLYNESMQLKTMLEETRNLMATSKNTHLRQIEQMESEELALQKKLRTEVIQLEDALAQVRKEYEMLRVEFEQNLAANEQTGPINREMRHLITSLQNHNHQLKGEVQRYKRKLKEMTAELAKLQPLANMHETIANIKEEPSNSSSGSSNSIPMNATVKEEVVIKKEKDDDHYRDEETDLREKGKSDAEIIRELKAQLKKSQEAQKELKLLLDMYKGAPKEQRDKAQLMAAEKKARAEVEELRNQIKKNLDNERKERRKLADEEALKKIKALEDQLQSLQKQLASQKQEEEALLNEMDITGQAFEDMQEQNLRLIQQLREKDDANFKLMSERIKSNQIHKLLREEKGMLTEQVMTFKNQVDAQNLVVRKLEEKERLLQTNVATVEKELSLRQQSSEMQKRKSIESAQSSADLRLHLEKYLSHLKEVQNTVAEKTSALQQEAFKTKRLQEDIISLRREVERGKKFELASTADEVLMEEIREYKEQLTCPSCKVKRKDAVLTKCFHVFCFDCLKTRYETRQRKCPKCNANFGANDFHRLYLA 892
BLAST of E3 ubiquitin protein ligase vs. TrEMBL
Match: A0A087T740 (E3 ubiquitin protein ligase (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_07010 PE=3 SV=1) HSP 1 Score: 561.222 bits (1445), Expect = 0.000e+0 Identity = 350/947 (36.96%), Postives = 563/947 (59.45%), Query Frame = 3
Query: 69 LMSKRPADFDETANDETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNHKDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLEGDSCEV------SETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLD--IPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTE--TNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKM----------------DNTNLXXXXXXXXXXXXRGVLLG-------LSDPSTFD---SLTSEEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEK-RLHEPDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYLT 2798
+ +KRPA D ++ + + K+ +E + G + + EE+DMK LQ QN+KL+E L ++++ + RIEQLE RQ D+ ++C VNRYWN L+ED +ILL+R + ++ + +E FL L WD+E+LE KL +RV S R ++++++AF++ +R +H + + ++ + S+ ++ SLD + Q V+L NEN L L L + LH +HH++ + E+ DK+ + E K+ ED EY L++T + +++ L+E T K+ L D D S + ++K+++L ELE+ KE RL ELE L +H+ L+ V +LK+ +Q +PESV++++ +YK L +QF++LY E++ ++ LE+ R + T++ HL+QIE+ME E +Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K E QR ++K KE+ A K++ + T+ +K V++ D D S+ E+I+ L+++L S+E KEL LL+ Y+ K+QRDKAQLMA+E R E + ++ R + N K R D + L++++ +E+ L+ Q+ LA KQ+E+A++N+ +T QA+ED+ EQN RL+Q L+EKD+ N KLM++R+K+NQ KLL+E++ + +Q+ K Q++A N+ + K EEKERL N+ T+EKE + RQQ+ E+ KRK++E Q++ DLR++L+KYLS LKE QT++ +K SA +EAFK +R++EE++ LRRK ER KK E ++ DEVL+ E++ YK+ LTC +CK +KDA+LTKCFHVFC CLKTRYE R RKCPKCNA FGAND+H +YL
Sbjct: 1 MSNKRPAPQDGSSQNSS------KRV--HFEPCRIGPVSTQEEMDMKVLQFQNRKLTERLEQRQRQEAELRQRIEQLEKRQTSDEAVLCVVNRYWNQLNEDLRILLQRFDAETSDEYENRNENEATTSFLALLGQWDREELEEKLAQRVQVSTRAVAKVLQAFDRIVQR----------NHKIML--------------ALKGDPSSEEDIPSLDDAVRQANVELQNENKNLHL------------LVTGLHEKHHQMNLKFAEMNDKLAAQEIANAELKNHIEDLEYDLSKTKQREEKLDMHLSECLTKLKSYNLTD-----------SDGRPPMSTVTQAKMEDLQKELEEQKELANNRLMELETLNTQHKEALKLVEKLKMDLQCLPESVVVETTEYKCLQSQFSVLYNESMQLKTLLEDTRNLMATSKNTHLRQIEQMESEELSMQKKLRTEVIQLEDALAQVRKEYEMLRVEFEQNLAANEQTGPINREMRHLITSLQNHNHQLKGEVQRYKRKLKEVTAELVKIQPLVNSHENAATIKEEQSNTTSASTNCPPMSATVKEEVIVKKEKEDDHYRDEEGSDIREKGKSDTEIIRELKAQLKKSQEAQKELKLLLDMYKGAPKEQRDKAQLMAAEKKARAEVEELRNQIKRNQDNERKERRKLADEEALKKIKILEDQLQSLQKSLASQKQEEEALLNEMDITGQAFEDMQEQNLRLIQQLREKDDANFKLMSERIKSNQIHKLLREEKSMLTEQVITLKNQVDAQNQVVRKLEEKERLLQTNVATVEKELSLRQQSSEMQKRKSIESAQSSADLRLHLEKYLSHLKEVQTTVAEKTSAVQQEAFKTKRLQEEIISLRRKVERGKKFELASTADEVLMEEVREYKEQLTCPSCKVKRKDAVLTKCFHVFCFDCLKTRYETRQRKCPKCNANFGANDFHRLYLA 892
BLAST of E3 ubiquitin protein ligase vs. TrEMBL
Match: A0A2R5LCT0 (E3 ubiquitin protein ligase OS=Ornithodoros turicata OX=34597 PE=3 SV=1) HSP 1 Score: 556.214 bits (1432), Expect = 1.032e-180 Identity = 336/915 (36.72%), Postives = 551/915 (60.22%), Query Frame = 3
Query: 198 LDMKTLQIQNKKLSECLLDQKQELNNHKDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLEGDSCEVSE------TAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKAL-KDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLK----MDNTNLXXXXXXXX---------------XXXXRGVLLG--LSDPSTFDSLTSEE----------------EMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE----PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYLT 2798
+DMKTLQ QN+KLS+ L + ++ + R+EQLE RQ D+ ++ VNRYWN L+ED ++LL+R + ++ + +E FL L WD+E+LE KL +RV S R ++++++AF++ +R +H V + + + NN E + P G ++E ++ +EL +EN + + + LH ++H L + E KD+ ++ LK +E +++ E+ EY L + +++ L E ++ K+L K + G+ ++ +KV+E+ ++LE+ +E A R+ ELE+L+ ++ +L+ V RLK+ ++ +PE+V++++ +YK L +QF++LY E++ ++ QLEE+R + T++ HL+ IE+ME E L+Q ++R++++ + + ++R EYE L +E +Q + + + PI EMR LI +L+ Q K E QR ++K KE A +LK M + + + + G + G L+ P ++ S+E E+I+ L+ +L S+E +E+ LL+ Y+ K+QRDK QLMA+E R E +E S+LK +E+ E D D ++++R +EET+ + LA KQ+E+A++N+ VT QA+ED+ EQN RL+Q L+EKD+ N KLM++R+K+NQ K+L+E++ +Q+ + Q+E+ N+ + K EEKERL N+L TLEKE + RQQA E+H+RKA+E Q+A DL+++L KYL+QLK++Q+ + D+A+ ++EAFK +R++EE++ LRRK ER KK E ++NTDEVL+ EI+ YK++LTC +CK +KDA+L KCFHVFC CLKTRYE R RKCPKCNA FG NDYH +YLT
Sbjct: 1 MDMKTLQFQNRKLSQRLEQRVKQEAELRARVEQLEKRQTSDEGLLTVVNRYWNQLNEDLRVLLQRFDAETSDEAEGRNEAAATTSFLGLLLHWDREELEEKLAQRVQVSTRAVAKVLQAFDRLVQR----------NHKVML--------------ALRGGAAAGNNEED-EAPSG--PDVDEAIRQSNAELQEENKNLHTMVTSLHEKNHLLSLKYQEHKDRADSLQLKKDELRNRVEELEYELGKVRAREEKLDAHLAECLQRLKSLPKASGSTSEEGSGALTVSVAQTSATCAKVEEVQSQLEEQRELAAARIMELEQLQHDYKESLKMVERLKMDLRSLPETVVVETAEYKCLQSQFSVLYNESMQLKTQLEESRQVLATSKNSHLRHIEQMESEELLMQKKLRTEVIRLEDALVQVRKEYEMLRIEFEQNLAATEQTGPINREMRHLITSLQSHNRQLKGEVQRYKRKLKEAAAEAHRLKQTLEMSHVAVTSTVPSSASSTSSEGGSSAGREDSTVAPGSVSGGPLTGPHQVEARRSDEDSHDDDLPTEKGKTDPELIRELKIQLKKSQEAQREMKLLLDMYKGAPKEQRDKVQLMAAEKKARAE---VEEHRSQLKKFVEQERKERRKLADEDAMKKIRSLEETVAALHKNLAAQKQEEEALLNEMEVTGQAFEDMQEQNLRLIQQLREKDDANFKLMSERIKSNQIHKMLQEEKGTLAEQVATLQAQVESQNQVVRKLEEKERLLQNSLATLEKELSLRQQAAEMHRRKAVESAQSAADLKLHLDKYLAQLKDAQSIVTDRAAVLSQEAFKTKRLQEEIISLRRKVERAKKFELASNTDEVLVEEIKEYKELLTCPSCKVKRKDAVLVKCFHVFCYDCLKTRYETRQRKCPKCNAPFGTNDYHRLYLT 885
BLAST of E3 ubiquitin protein ligase vs. Ensembl Cavefish
Match: rnf20 (ring finger protein 20 [Source:NCBI gene;Acc:103034820]) HSP 1 Score: 265.388 bits (677), Expect = 1.414e-74 Identity = 153/354 (43.22%), Postives = 233/354 (65.82%), Query Frame = 3
Query: 1740 EEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE-PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKK-LEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
E E +K +++EL ++E +E+ LL+ Y++ K+QRDK QLMA+E + E ++ + L+ + + D + LR++R +EE + +KL+LAKQ+EDA++++ VT QA+ED+ EQN RL+Q L+EKD+ N KLM++R+K+NQ KLLKE+++ DQ+ KTQ++A + + K EEKERL ++ E+E R QAL+++KRKA + +E++R L+ +L + + + + ++ +E+F RR +E++ LRRK E+ KK E+ N DE+L EI YK LTC C S KDA+LTKCFHVFC C+KTRY+ R RKCPKCNA FGAND+H IY+
Sbjct: 608 EVEQLKIVRAELKKAQESQREMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAEGEELRQRLRDLEERERREGKKMADEEALRKIRSVEEQIDILNKKLSLAKQEEDALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQLLTLKTQVDAQLQVVRKLEEKERLLQGTISAAERELGLRTQALDMNKRKAQDSALLSEEVRSQLEGVQQRLNAVREEVIENSISREKESFNARRAQEDISKLRRKIEKAKKPAENIRNGDEILNEEINEYKARLTCPCCNSRVKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYI 961
BLAST of E3 ubiquitin protein ligase vs. Ensembl Cavefish
Match: rnf20 (ring finger protein 20 [Source:NCBI gene;Acc:103034820]) HSP 1 Score: 265.003 bits (676), Expect = 3.431e-74 Identity = 153/354 (43.22%), Postives = 233/354 (65.82%), Query Frame = 3
Query: 1740 EEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE-PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKK-LEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
E E +K +++EL ++E +E+ LL+ Y++ K+QRDK QLMA+E + E ++ + L+ + + D + LR++R +EE + +KL+LAKQ+EDA++++ VT QA+ED+ EQN RL+Q L+EKD+ N KLM++R+K+NQ KLLKE+++ DQ+ KTQ++A + + K EEKERL ++ E+E R QAL+++KRKA + +E++R L+ +L + + + + ++ +E+F RR +E++ LRRK E+ KK E+ N DE+L EI YK LTC C S KDA+LTKCFHVFC C+KTRY+ R RKCPKCNA FGAND+H IY+
Sbjct: 659 EVEQLKIVRAELKKAQESQREMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAEGEELRQRLRDLEERERREGKKMADEEALRKIRSVEEQIDILNKKLSLAKQEEDALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQLLTLKTQVDAQLQVVRKLEEKERLLQGTISAAERELGLRTQALDMNKRKAQDSALLSEEVRSQLEGVQQRLNAVREEVIENSISREKESFNARRAQEDISKLRRKIEKAKKPAENIRNGDEILNEEINEYKARLTCPCCNSRVKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYI 1012 HSP 2 Score: 181.8 bits (460), Expect = 1.743e-46 Identity = 144/515 (27.96%), Postives = 260/515 (50.49%), Query Frame = 3
Query: 165 VKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRL-------------------------EGDSCE-------VSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQ 1610
+K G++ + EE D+K LQ++N+KL E L DQ+Q + + ++R+E+LE RQA DD + +NRYWN LD++ +++ + +GDS + E A FL LAS E++E +L+ERV S++ SR+VE +E S TVD +K + +ES ++ N ++ + L L EN + +L +L +H + SE+ + ++ E + E+ ++ + R NR+N L E E+ + ++ G I K K +E+ +ELE+ +E RL+EL+KL++ Q ++E +K+++ E ++ ++ +Y+ L +QF++LY E++ ++AQL+E RA + T R L+Q++ ME +E +Q ++R++++ + + +++R EYE L +E +QT+ N+ + PI EMR LI TL+ Q K E + + + +E Q
Sbjct: 45 IKLGSVANSEEQDIKALQVKNRKLGEAL-DQRQVIEDELRERVERLETRQATDDASLLILNRYWNQLDDNMRLIARGYDQAGSEPVDSQPSEPRSLKPGTPEPDGDSNQERAKDRGQGEGATSFLAMLASSTSEEMEAELQERVESSRKQASRVVEIYE-----------------------SLKSTVDQLK-KSLESG--TEGNAALWEVAASLNTL-----------LSNENEHLRQLTDDLMQKHSHMTSESRSLGRAATRAENRINELQVLIEELQWDTEKVRRRENRLNTHLGEVLERVNSKGYKVC------GEASSVCGTITINKRKFEEMNSELEENRELAENRLSELQKLQQDLQTVVQENNNMKMELLSRAEGLVRETAEYRCLQSQFSVLYNESLVLKAQLDETRARLNTTRAARLRQLDHMENDEVSLQRKVRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISTLQTHNQQMKGEVVKYKVRLRESQ 515
BLAST of E3 ubiquitin protein ligase vs. Ensembl Cavefish
Match: rnf20 (ring finger protein 20 [Source:NCBI gene;Acc:103034820]) HSP 1 Score: 265.003 bits (676), Expect = 3.431e-74 Identity = 153/354 (43.22%), Postives = 233/354 (65.82%), Query Frame = 3
Query: 1740 EEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE-PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKK-LEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
E E +K +++EL ++E +E+ LL+ Y++ K+QRDK QLMA+E + E ++ + L+ + + D + LR++R +EE + +KL+LAKQ+EDA++++ VT QA+ED+ EQN RL+Q L+EKD+ N KLM++R+K+NQ KLLKE+++ DQ+ KTQ++A + + K EEKERL ++ E+E R QAL+++KRKA + +E++R L+ +L + + + + ++ +E+F RR +E++ LRRK E+ KK E+ N DE+L EI YK LTC C S KDA+LTKCFHVFC C+KTRY+ R RKCPKCNA FGAND+H IY+
Sbjct: 659 EVEQLKIVRAELKKAQESQREMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAEGEELRQRLRDLEERERREGKKMADEEALRKIRSVEEQIDILNKKLSLAKQEEDALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQLLTLKTQVDAQLQVVRKLEEKERLLQGTISAAERELGLRTQALDMNKRKAQDSALLSEEVRSQLEGVQQRLNAVREEVIENSISREKESFNARRAQEDISKLRRKIEKAKKPAENIRNGDEILNEEINEYKARLTCPCCNSRVKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYI 1012 HSP 2 Score: 181.8 bits (460), Expect = 1.743e-46 Identity = 144/515 (27.96%), Postives = 260/515 (50.49%), Query Frame = 3
Query: 165 VKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRL-------------------------EGDSCE-------VSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQ 1610
+K G++ + EE D+K LQ++N+KL E L DQ+Q + + ++R+E+LE RQA DD + +NRYWN LD++ +++ + +GDS + E A FL LAS E++E +L+ERV S++ SR+VE +E S TVD +K + +ES ++ N ++ + L L EN + +L +L +H + SE+ + ++ E + E+ ++ + R NR+N L E E+ + ++ G I K K +E+ +ELE+ +E RL+EL+KL++ Q ++E +K+++ E ++ ++ +Y+ L +QF++LY E++ ++AQL+E RA + T R L+Q++ ME +E +Q ++R++++ + + +++R EYE L +E +QT+ N+ + PI EMR LI TL+ Q K E + + + +E Q
Sbjct: 45 IKLGSVANSEEQDIKALQVKNRKLGEAL-DQRQVIEDELRERVERLETRQATDDASLLILNRYWNQLDDNMRLIARGYDQAGSEPVDSQPSEPRSLKPGTPEPDGDSNQERAKDRGQGEGATSFLAMLASSTSEEMEAELQERVESSRKQASRVVEIYE-----------------------SLKSTVDQLK-KSLESG--TEGNAALWEVAASLNTL-----------LSNENEHLRQLTDDLMQKHSHMTSESRSLGRAATRAENRINELQVLIEELQWDTEKVRRRENRLNTHLGEVLERVNSKGYKVC------GEASSVCGTITINKRKFEEMNSELEENRELAENRLSELQKLQQDLQTVVQENNNMKMELLSRAEGLVRETAEYRCLQSQFSVLYNESLVLKAQLDETRARLNTTRAARLRQLDHMENDEVSLQRKVRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISTLQTHNQQMKGEVVKYKVRLRESQ 515
BLAST of E3 ubiquitin protein ligase vs. Ensembl Cavefish
Match: rnf40 (ring finger protein 40 [Source:NCBI gene;Acc:103040489]) HSP 1 Score: 144.821 bits (364), Expect = 6.785e-35 Identity = 116/409 (28.36%), Postives = 210/409 (51.34%), Query Frame = 3
Query: 417 SETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENL----RSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLK 1631
SE+A+ FL L + +E+L +L++R+ FSK ++ +V F++ + E L +S + E G + +TN T L +EN R+ L L +HH++ E E+ DK+ + K+ E ++ ED ++ + + + ++N+ L E AL+ + P G + K + L ELE +E R+ ELEKL+++ Q + E +LK+ ++ IPE V+ ++ +Y+ L +QF++LY E++ ++ QL+EARA + T + HL+QIE ME +E +Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K + QR ++K +E Q +KLK
Sbjct: 216 SESAKAFLSTLDNSSEEELSLQLQDRMQFSKGAVACMVCIFDRLHSSIEELCTRVQSAVCEDDGQREIIATNRT------------------------------------------LLEENNRLHDLAFSLQGRHHKMSMEYNELVDKVTSSETKVSEMETAVEDLQWDIEKLRKREQKLNKHLAE------ALEQLNSGYYASGSSGGLPGGQITLNIQKFELLNAELEQNQELANSRMAELEKLQQELQEAVRENEKLKIDLRNIPEEVVKETPEYRCLQSQFSLLYNESLGVKTQLDEARALLLTAKNAHLRQIEHMESDELSLQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLISSLQNHNHQLKGDVQRYKRKLRETQMEINKLK 576 HSP 2 Score: 76.2554 bits (186), Expect = 1.290e-13 Identity = 39/81 (48.15%), Postives = 57/81 (70.37%), Query Frame = 3
Query: 159 EQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLE 398
E ++ G + S EE+DMK +Q +NKKL E L+Q+Q + + +++IE+LE RQA DD + VNRYW LDE+ Q LL+R+E
Sbjct: 35 EPIRIGGVSSTEEMDMKVIQFKNKKLFE-RLEQRQAIEDELREKIEKLEKRQATDDTTLLIVNRYWTQLDENVQELLQRVE 114
BLAST of E3 ubiquitin protein ligase vs. Ensembl Cavefish
Match: rnf40 (ring finger protein 40 [Source:NCBI gene;Acc:103040489]) HSP 1 Score: 126.331 bits (316), Expect = 3.551e-29 Identity = 70/193 (36.27%), Postives = 127/193 (65.80%), Query Frame = 3
Query: 1074 ELMN-ELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMDNTNL 1649
EL+N ELE +E R+ ELEKL+++ Q + E +LK+ ++ IPE V+ ++ +Y+ L +QF++LY E++ ++ QL+EARA + T + HL+QIE ME +E +Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ +Q K + QR ++K +E Q +K+++ + L
Sbjct: 278 ELLNAELEQNQELANSRMAELEKLQQELQEAVRENEKLKIDLRNIPEEVVKETPEYRCLQSQFSLLYNESLGVKTQLDEARALLLTAKNAHLRQIEHMESDELSLQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLISSLQNHNHQLKGDVQRYKRKLRETQMEINKVEVSFSPL 470
BLAST of E3 ubiquitin protein ligase vs. Ensembl Yeast
Match: BRE1 (E3 ubiquitin ligase; forms heterodimer with Rad6p to regulate K63 polyubiquitination in response to oxidative stress and to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control; interacts with RNA-binding protein Npl3p, linking histone ubiquitination to mRNA processing; Bre1p-dependent histone ubiquitination promotes pre-mRNA splicing [Source:SGD;Acc:S000002232]) HSP 1 Score: 73.9442 bits (180), Expect = 6.947e-14 Identity = 28/72 (38.89%), Postives = 42/72 (58.33%), Query Frame = 3
Query: 2580 NNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
++DE L+ E+ ++ ++ CS C N K+ + C HVFC +C K R R RKCP CN F +ND ++L
Sbjct: 629 GSSDEALVEELANFRTLVYCSLCSKNWKNMAIKTCGHVFCENCCKERLAARMRKCPTCNKAFSSNDLLTVHL 700
BLAST of E3 ubiquitin protein ligase vs. Ensembl Nematostella
Match: EDO32394 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SV65]) HSP 1 Score: 248.825 bits (634), Expect = 8.749e-75 Identity = 144/318 (45.28%), Postives = 207/318 (65.09%), Query Frame = 3
Query: 1812 LLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEK-RLHEPDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNAT 2762
LL+ Y+ KD RDK +L+ASE A++ E S K V L+ LEK + D + +RR++ EET+++ Q+ LA KQ+E A++ + T Q YE++ EQN RLLQ L+EKD+ NLKLM++R+K+NQ K ++E++ + D + EA + + KQEE+ER N + +EKE RQQ ++LHKRKA+E Q A+DL++ L QL+E+Q L +K EAF+ +R++EE + L+RK E+ KK++ DEVL+ E++ YK LTC C + KKDAILTKCFHVFC CLKTRY+ R RKCP +AT
Sbjct: 4 LLDVYKGAAKDLRDKVELLASEKALKEEIDSIKTRVEGLEQELEKHKGAMADEEAIRRLKMAEETIENLQKNLAATKQEEAALLAEMEFTGQEYEEMQEQNVRLLQQLREKDDANLKLMSERIKSNQIQKQIREEKDVLADHVTSLNGYREAQCQLVKKQEERERALQNAVTAMEKELNLRQQTMDLHKRKAVESAQNAQDLKIRLDALQQQLQEAQDGLNEKTRCVEEEAFRAKRVQEECVSLKRKLEKQKKIDLYGAADEVLLEEVRQYKARLTCPCCNTRKKDAILTKCFHVFCYECLKTRYDTRQRKCPNLSAT 321
BLAST of E3 ubiquitin protein ligase vs. Ensembl Medaka
Match: rnf20 (ring finger protein 20 [Source:NCBI gene;Acc:101156585]) HSP 1 Score: 263.848 bits (673), Expect = 1.020e-73 Identity = 153/361 (42.38%), Postives = 238/361 (65.93%), Query Frame = 3
Query: 1719 TFDSLTSEEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE-PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKK-LEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
T S E E +K +++EL ++E +E+ LL+ Y++ K+QRDK QLMA+E + E+ ++ + L+ + + D + LR++R +EE + +KL++AKQ+EDA++++ VT QA+ED+ EQN RL+Q L+EKD+ N KLM++R+K+NQ KLLKE+++ DQ+ KTQ++A + + K EEKERL ++ E+E R QAL+++KRKA + +++LR L++ +LK + + + + ++ +E+F RR +E++ +RRK E+ KK E +N D++L EI YK LTC C S KDA+LTKCFHVFC C+KTRY+ R RKCPKCNA FGAND+H IY+
Sbjct: 663 TGGSKRKEMEQLKIIRAELKKAQESQREMKLLLDMYRSAPKEQRDKVQLMAAEKKTKSEAEELRQRLRELEERERREGKKMADEEALRKIRSVEEQIDILNKKLSIAKQEEDALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQLLTLKTQVDAQLQVVRKLEEKERLLQGTISAAERELNLRTQALDMNKRKAQDSALLSDELRAQLEQVQQKLKLVREEVVENSISREKESFNARRAQEDISKMRRKIEKAKKPAEKISNGDDILNEEINEYKARLTCPCCNSRVKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYI 1023 HSP 2 Score: 186.037 bits (471), Expect = 7.757e-48 Identity = 148/522 (28.35%), Postives = 261/522 (50.00%), Query Frame = 3
Query: 165 VKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLEGDSCEV---------------------------------SETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKL 1628
+K G + + EE D+K LQ +N+KL E L DQ+Q + + ++RIE+LE RQA DD + +NRYWN DE+ +++++R + S E ET+ FL LAS E++E +L+ERV S++ R+V+ +E NL+S TVD ++ E E S + S KL + L EN R+ +L +L +H + SE+ + ++ E +++ E+ ++ + + R NR+N L+E E+ + ++ G I K K +E+ +ELE+ +E RL EL+KL++ Q +E + ++ E ++ D+ +Y+ L +QF++LY E++ +++QL+E RA + T R L+Q+E ME +E +Q ++R+++ + + ++++ EYE L +E +QT+ N+ + PI EMR LI TL+ Q K E + + + +E QA +K+
Sbjct: 49 IKLGGVSNSEEQDIKALQAKNRKLGEAL-DQRQVIEDELRERIERLETRQATDDASLLILNRYWNQFDENVRLIIRRYDQGSSEPEKSPTGEGRSLKPETPEPDGDSNQERAKDRTHQGETSNSFLATLASSTSEEMEAELQERVESSQKQAQRVVDVYE-------NLKS----------------TVDQLQTELDSGTEGSFWQVVS---------------KLNVL-LTSENERLQQLTEDLKQRHSHMTSESRALGRATNKADQRVTELQARIEELQWDMEKIRRRENRLNAHLSEILERVNSKGYKVC------GEASSVCGTITINKRKFEEMNSELEENRELAENRLIELQKLQQDLQNVQQENNNVTAEILSRAEGLVKDTSEYRCLQSQFSVLYNESLTLKSQLDETRARLNTTRTARLRQLEHMENDEVALQRKVRTEVFQLEDTLAQVKKEYEMLRIEFEQTLAANEQAGPINREMRHLISTLQTHNQQLKGEVVKYKLRLRETQAELNKV 524
BLAST of E3 ubiquitin protein ligase vs. Ensembl Medaka
Match: rnf20 (ring finger protein 20 [Source:NCBI gene;Acc:101156585]) HSP 1 Score: 263.077 bits (671), Expect = 2.644e-73 Identity = 153/361 (42.38%), Postives = 238/361 (65.93%), Query Frame = 3
Query: 1719 TFDSLTSEEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHE-PDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKK-LEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYL 2795
T S E E +K +++EL ++E +E+ LL+ Y++ K+QRDK QLMA+E + E+ ++ + L+ + + D + LR++R +EE + +KL++AKQ+EDA++++ VT QA+ED+ EQN RL+Q L+EKD+ N KLM++R+K+NQ KLLKE+++ DQ+ KTQ++A + + K EEKERL ++ E+E R QAL+++KRKA + +++LR L++ +LK + + + + ++ +E+F RR +E++ +RRK E+ KK E +N D++L EI YK LTC C S KDA+LTKCFHVFC C+KTRY+ R RKCPKCNA FGAND+H IY+
Sbjct: 706 TGGSKRKEMEQLKIIRAELKKAQESQREMKLLLDMYRSAPKEQRDKVQLMAAEKKTKSEAEELRQRLRELEERERREGKKMADEEALRKIRSVEEQIDILNKKLSIAKQEEDALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQLLTLKTQVDAQLQVVRKLEEKERLLQGTISAAERELNLRTQALDMNKRKAQDSALLSDELRAQLEQVQQKLKLVREEVVENSISREKESFNARRAQEDISKMRRKIEKAKKPAEKISNGDDILNEEINEYKARLTCPCCNSRVKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYI 1066 HSP 2 Score: 186.037 bits (471), Expect = 8.748e-48 Identity = 148/522 (28.35%), Postives = 261/522 (50.00%), Query Frame = 3
Query: 165 VKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLEGDSCEV---------------------------------SETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKL 1628
+K G + + EE D+K LQ +N+KL E L DQ+Q + + ++RIE+LE RQA DD + +NRYWN DE+ +++++R + S E ET+ FL LAS E++E +L+ERV S++ R+V+ +E NL+S TVD ++ E E S + S KL + L EN R+ +L +L +H + SE+ + ++ E +++ E+ ++ + + R NR+N L+E E+ + ++ G I K K +E+ +ELE+ +E RL EL+KL++ Q +E + ++ E ++ D+ +Y+ L +QF++LY E++ +++QL+E RA + T R L+Q+E ME +E +Q ++R+++ + + ++++ EYE L +E +QT+ N+ + PI EMR LI TL+ Q K E + + + +E QA +K+
Sbjct: 92 IKLGGVSNSEEQDIKALQAKNRKLGEAL-DQRQVIEDELRERIERLETRQATDDASLLILNRYWNQFDENVRLIIRRYDQGSSEPEKSPTGEGRSLKPETPEPDGDSNQERAKDRTHQGETSNSFLATLASSTSEEMEAELQERVESSQKQAQRVVDVYE-------NLKS----------------TVDQLQTELDSGTEGSFWQVVS---------------KLNVL-LTSENERLQQLTEDLKQRHSHMTSESRALGRATNKADQRVTELQARIEELQWDMEKIRRRENRLNAHLSEILERVNSKGYKVC------GEASSVCGTITINKRKFEEMNSELEENRELAENRLIELQKLQQDLQNVQQENNNVTAEILSRAEGLVKDTSEYRCLQSQFSVLYNESLTLKSQLDETRARLNTTRTARLRQLEHMENDEVALQRKVRTEVFQLEDTLAQVKKEYEMLRIEFEQTLAANEQAGPINREMRHLISTLQTHNQQLKGEVVKYKLRLRETQAELNKV 567
BLAST of E3 ubiquitin protein ligase vs. Ensembl Medaka
Match: rnf40 (ring finger protein 40 [Source:NCBI gene;Acc:101166749]) HSP 1 Score: 153.295 bits (386), Expect = 1.652e-37 Identity = 119/406 (29.31%), Postives = 212/406 (52.22%), Query Frame = 3
Query: 414 VSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLK 1631
+SE A+ FL L +E+L L++R+ FSK I++LV F++ + R +D++ E+ K DN+ D+I LE EN R+ +L + LH +HH++ E E+ DK+ + K+ E ++ ED ++ + + ++N+ L E E+ K+ + G + K + L ELE +E R+ ELEKL+ + Q + E +LK+ ++ IPE V+ ++++YK L +QF++LY E++ ++ QL+EARA + T + HL+QIE ME +E +Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ Q K + QR ++K +E Q +KL+
Sbjct: 211 LSENAKGFLAVLEQSTEEELALHLQDRMQFSKDAIAQLVCVFDRLHSR-----------------------IDSMCRAAQEAACKDDNH----------PDIIQNRTLLE------ENTRLHELSTLLHGRHHKMSMEYNELVDKVTSAETKVSEMETTVEDLQWDIEKLRNREQKLNKHLAEAMEQLKSGY------SSSGSSAGLAGGQITLNIQKFESLNAELEHNQELANSRMAELEKLQAELQEAVRESEKLKMDLRNIPEEVVKETLEYKCLQSQFSLLYNESLGVKTQLDEARALLLTAKNAHLRQIEHMESDELSLQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLISSLQNHNLQLKGDVQRYKRKLRETQMEINKLR 571 HSP 2 Score: 72.4034 bits (176), Expect = 1.704e-12 Identity = 43/118 (36.44%), Postives = 67/118 (56.78%), Query Frame = 3
Query: 75 SKRPADFDETANDETCPNIPVKQFVSS-------YEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLEGD 404
KRP+ D + P P K+ E ++ + S EE+DMK +Q +NKKL E L+Q+Q + + +++IE+LE RQA DD + VNRYW+ L+E ++L KR+E +
Sbjct: 7 GKRPSGGD------SPPGPPEKKSKKEEKTTTTLIEPIRIAGVSSTEEMDMKVIQFKNKKLCE-RLEQRQAMEDELREKIEKLEKRQATDDTTLLIVNRYWSQLEESVRLLRKRIEPE 117
BLAST of E3 ubiquitin protein ligase vs. Ensembl Medaka
Match: rnf40 (ring finger protein 40 [Source:NCBI gene;Acc:101166749]) HSP 1 Score: 109.383 bits (272), Expect = 6.371e-24 Identity = 55/151 (36.42%), Postives = 100/151 (66.23%), Query Frame = 3
Query: 1185 KLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMD 1637
K+ ++ IPE V+ ++++YK L +QF++LY E++ ++ QL+EARA + T + HL+QIE ME +E +Q ++R++++ + + +++R EYE L +E +Q + N+ + PI EMR LI +L+ Q K + QR ++K +E Q +K+ D
Sbjct: 329 KMDLRNIPEEVVKETLEYKCLQSQFSLLYNESLGVKTQLDEARALLLTAKNAHLRQIEHMESDELSLQKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQNLAANEQAGPINREMRHLISSLQNHNLQLKGDVQRYKRKLRETQMEINKVDDD 479 HSP 2 Score: 74.3294 bits (181), Expect = 4.168e-13 Identity = 89/342 (26.02%), Postives = 151/342 (44.15%), Query Frame = 3
Query: 75 SKRPADFDETANDETCPNIPVKQFVSS-------YEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNH-KDRIEQLEDRQAKDDEMMCTVNRYWN----MLDEDCQI--------------------------LLKRLE--GDSCEVSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNK 980
KRP+ D + P P K+ E ++ + S EE+DMK +Q +NKKL E L+Q+Q + + +++IE+LE RQA DD + VNRYW+ +L E Q+ LL + E +S E A+ FL L +E+L L++R+ FSK I++LV F++ +++ + V+ + + K DN+ D+I LE EN R+ +L + LH +HH++ E E+ DK+ + K+ E ++ ED ++ + + ++N+ L E E+ K
Sbjct: 7 GKRPSGGD------SPPGPPEKKSKKEEKTTTTLIEPIRIAGVSSTEEMDMKVIQFKNKKLCE-RLEQRQAMEDELREKIEKLEKRQATDDTTLLIVNRYWSQVPYLLHESEQMDQQQVEERQEKTQEEQQPPPPAGSEELLTQTEPAPESKTGYENAKGFLAVLEQSTEEELALHLQDRMQFSKDAIAQLVCVFDR----------LMLQRNCVQFLELCVSVLLAC---------KDDNH----------PDIIQNRTLLE------ENTRLHELSTLLHGRHHKMSMEYNELVDKVTSAETKVSEMETTVEDLQWDIEKLRNREQKLNKHLAEAMEQLK 306
BLAST of E3 ubiquitin protein ligase vs. Planmine SMEST
Match: SMESG000069086.1 (SMESG000069086.1) HSP 1 Score: 1782.3 bits (4615), Expect = 0.000e+0 Identity = 909/909 (100.00%), Postives = 909/909 (100.00%), Query Frame = 3
Query: 72 MSKRPADFDETANDETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNHKDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLEGDSCEVSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKXXXXXXXXXXXXSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMDNTNLXXXXXXXXXXXXRGVLLGLSDPSTFDSLTSEEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHEPDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYLT 2798
MSKRPADFDETANDETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNHKDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLEGDSCEVSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKGGTDTEDDPDGPSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMDNTNLEKEYMETKEKMKRGVLLGLSDPSTFDSLTSEEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHEPDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYLT
Sbjct: 1 MSKRPADFDETANDETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIQNKKLSECLLDQKQELNNHKDRIEQLEDRQAKDDEMMCTVNRYWNMLDEDCQILLKRLEGDSCEVSETAEYFLKQLASWDKEQLECKLKERVHFSKRIISRLVEAFEKENKRFENLRSKLVESHGVKIEKSTNETVDNVKFEKVESNEKSDNNLESLDIPQGIVDLINENLKLELSELHKENIRVTKLCSELHAQHHELKSENLEIKDKMENYSLKLEEWKSKYEDTEYCLAETNRDYNRMNRWLTETNEKNKALKDEIVLLKGGTDTEDDPDGPSAIAKSKVKELMNELEDAKECGARRLTELEKLEKKHQLNLEEVTRLKLQMQEIPESVILDSVQYKSLHAQFNILYKEAVNMQAQLEEARATIQTNRQHHLKQIEEMELNESLIQNQMRSDMMAMREEYSKLRTEYEALDLELKQTICHNQHSVPIASEMRSLIDTLRVQANQAKNEAQRSRKKYKELQATYDKLKMDNTNLEKEYMETKEKMKRGVLLGLSDPSTFDSLTSEEEMIKNLQSELTLSREKVKELTSLLNAYQNFTKDQRDKAQLMASENAVRVESLSYKEEVSRLKSNLEKRLHEPDPDQLRRVRKMEETLKDYQQKLALAKQQEDAMINDSTVTIQAYEDVLEQNSRLLQTLKEKDENNLKLMTDRMKANQTTKLLKEDQQLAEDQIRMYKTQIEALNRSLMKQEEKERLFLNNLNTLEKESTARQQALELHKRKAMEYQQTAEDLRMNLQKYLSQLKESQTSLQDKASAQAREAFKHRRIKEELMILRRKYERIKKLEHSNNTDEVLITEIQTYKDMLTCSTCKSNKKDAILTKCFHVFCLSCLKTRYEMRNRKCPKCNATFGANDYHHIYLT 909 The following BLAST results are available for this feature:
BLAST of E3 ubiquitin protein ligase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of E3 ubiquitin protein ligase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 2
BLAST of E3 ubiquitin protein ligase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 2
BLAST of E3 ubiquitin protein ligase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of E3 ubiquitin protein ligase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of E3 ubiquitin protein ligase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of E3 ubiquitin protein ligase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of E3 ubiquitin protein ligase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of E3 ubiquitin protein ligase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of E3 ubiquitin protein ligase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of E3 ubiquitin protein ligase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of E3 ubiquitin protein ligase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of E3 ubiquitin protein ligase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 4
BLAST of E3 ubiquitin protein ligase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30027325 ID=SMED30027325|Name=E3 ubiquitin protein ligase|organism=Schmidtea mediterranea sexual|type=transcript|length=2882bpback to top protein sequence of SMED30027325-orf-1 >SMED30027325-orf-1 ID=SMED30027325-orf-1|Name=SMED30027325-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=910bp MSKRPADFDETANDETCPNIPVKQFVSSYEQVKFGNIRSYEELDMKTLQIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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