SMED30027324

Overview
NameSMED30027324
Smed IDSMED30027324
Length (bp)920
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30027324 (SMED30027324) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30027324 (SMED30027324) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30027324 (SMED30027324) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30027324 (SMED30027324) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30027324

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30027324h1SMcG0013898 SmedASXL_008991SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30027324h1SMcG0013898 dd_Smed_v4_9968_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30027324h1SMcG0013898 dd_Smed_v4_9968_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30027324h1SMcG0013898 dd_Smed_v4_9968_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30027324h1SMcG0013898 dd_Smed_v4_9968_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
whole organismSMED30027324h1SMcG0013898 dd_Smed_v4_9968_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult colorimetric in situ hybridization evidence
dorsal region of the epidermisSMED30027324h1SMcG0013898 dd_Smed_v4_9968_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30027324 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.011589:2068..6829 -4480
Homology
[Close]
BLAST of SMED30027324 vs. Ensembl Human
Match: AC013470.2 (novel protein)

HSP 1 Score: 72.0182 bits (175), Expect = 2.255e-14
Identity = 58/210 (27.62%), Postives = 95/210 (45.24%), Query Frame = 2 
Query:  203 SIWDRLPPDLRLQSFQPCPIKRRLLLPTKIRNKPSEESQDKTRISVSRSSL----FTSKSDCGKSNNFIS---HFKIQRPFSAKIDFCKSGMYPKETYRPPQPFDYRGLKPIKDLGLPEFTMGIEKDPGNILLNTKSLGITYGPLNEDMVFRDTTGKSMYESIKQDPKWNSQNYLPNPPWPNIPKEFTRHRVIYQDPHAAFLERVETVLT 811
            ++W+  PPD   + +    I  R        +KP +E + + +IS   + L      +      +  FI+   H  +Q+   AK+ F KSG YP+  Y  P+P D+R  +P    GLP F    EKDP  +   ++ L   +G        + T+ +        +  W+S+  L   PWP     +TRHR   +D ++AF++RVE   T
Sbjct:    4 NVWEIKPPDFSYKLYTSLRIPER-------SSKPIKEEKRRKKISFPETMLHLPSIRNHPKEVTAPKFITTFPHLDLQK---AKLMFVKSGQYPRGVYVNPKPHDFRQYQP----GLPNFETTYEKDPFGLKFKSQHLSTVHGYQLPKDDKQKTSTERFITHKHCECTWDSKLILTKAPWPVRSASYTRHRR-QRDAYSAFMDRVEEKFT 198
[Close]
BLAST of SMED30027324 vs. Ensembl Human
Match: AC013470.2 (novel protein)

HSP 1 Score: 71.633 bits (174), Expect = 6.959e-14
Identity = 58/210 (27.62%), Postives = 95/210 (45.24%), Query Frame = 2 
Query:  203 SIWDRLPPDLRLQSFQPCPIKRRLLLPTKIRNKPSEESQDKTRISVSRSSL----FTSKSDCGKSNNFIS---HFKIQRPFSAKIDFCKSGMYPKETYRPPQPFDYRGLKPIKDLGLPEFTMGIEKDPGNILLNTKSLGITYGPLNEDMVFRDTTGKSMYESIKQDPKWNSQNYLPNPPWPNIPKEFTRHRVIYQDPHAAFLERVETVLT 811
            ++W+  PPD   + +    I  R        +KP +E + + +IS   + L      +      +  FI+   H  +Q+   AK+ F KSG YP+  Y  P+P D+R  +P    GLP F    EKDP  +   ++ L   +G        + T+ +        +  W+S+  L   PWP     +TRHR   +D ++AF++RVE   T
Sbjct:   56 NVWEIKPPDFSYKLYTSLRIPER-------SSKPIKEEKRRKKISFPETMLHLPSIRNHPKEVTAPKFITTFPHLDLQK---AKLMFVKSGQYPRGVYVNPKPHDFRQYQP----GLPNFETTYEKDPFGLKFKSQHLSTVHGYQLPKDDKQKTSTERFITHKHCECTWDSKLILTKAPWPVRSASYTRHRR-QRDAYSAFMDRVEEKFT 250
[Close]
BLAST of SMED30027324 vs. Ensembl Zebrafish
Match: si:dkey-30e9.6 (si:dkey-30e9.6 [Source:ZFIN;Acc:ZDB-GENE-131121-308])

HSP 1 Score: 61.6178 bits (148), Expect = 1.076e-10
Identity = 44/149 (29.53%), Postives = 72/149 (48.32%), Query Frame = 2 
Query:  380 KSNNFISHFKIQRPFSAKIDFCKSGMYPKETYRPPQPFDYRGLKPIKDLGLPEFTMGIEKDPGNILLNTKSLGITYGPLNEDMVFRDTTGKSMYESIK-QDPKWNSQNYLPNPPWPNIPKEFTRHRVIYQDPHAAFLERVETVLTGKWK 823
            K   FI+ +K       K+ F + G YP   Y+ P+P ++R   P ++ G+P+    +EKDPG +        I   P   D+ +      S  ++ K  + KW+ +  LP  PWP     +TRHR   +  ++A ++RVE  LT  W+
Sbjct:  104 KERPFITFYKPPDYLQTKLLFVRGGTYPVGPYKNPKPHNFR---PCEE-GMPDMVTSLEKDPGCMKSQCLKTVIDSHP---DLNYSCGDTISKIDTFKPAELKWDPRLILPKSPWPPKSASYTRHRR-RRGVYSALMDRVEEKLTNSWR 244
[Close]
BLAST of SMED30027324 vs. TrEMBL
Match: A0A267ERK9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig032252g1 PE=4 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 4.424e-33
Identity = 82/245 (33.47%), Postives = 123/245 (50.20%), Query Frame = 2 
Query:  131 FDYSKFYFADRDKSFHASSPLKTKSIWDRLPPDLRLQSFQPCPIKR---------RLLLPTKIRNKPSEESQDKTRISVSRSSLFTSKSDCGKSNNFISHFKIQRPFSAKIDFCKSGMYPKETYRPPQPFDYRGLKPIKDLGLPEFTMGIEKDPGNILLNTKSLGITYGPLNEDMVFRDTTGKSMYESIKQDPKWNSQNYLPNPPWPNIPKEFTRHRVIYQDPHAAFLERVETVLTGKWKNEKLS 838
            FD  +     R K F    P+    IW R PPD R Q+++P P KR         R  L +  R + +       R+     +    K    +     + F++ RP +AK  F + G  P+E Y    P D+R   PI+ LGLPEFT  IEKDP NI   +++     GPL+E    RD +G +M+ +++Q PKW+ + +L   P+PNI  E+TR+R   ++P   F++ VE  L  +W  EKL+
Sbjct:   54 FDARQELQKTRSKHFSVPPPVPDDDIWRRPPPDFRPQAWEPRPPKRNSRDAVQPWRYRLESDARYEAAAAVAPGDRLPALGQTAAQKKQQQQQKQRMQTLFRLMRPNTAKQRFVQEGALPREPYGTVGPHDFRQYPPIQSLGLPEFTTAIEKDPNNIRFLSRNRDTIVGPLHEIKNMRDASG-TMWSAVRQPPKWDPRLHLQQNPFPNISCEYTRYRPRNRNPFDGFMDTVEAGLEKRWLAEKLA 297
[Close]
BLAST of SMED30027324 vs. TrEMBL
Match: A0A267G1E7 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig032252g3 PE=4 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 6.879e-33
Identity = 81/245 (33.06%), Postives = 123/245 (50.20%), Query Frame = 2 
Query:  131 FDYSKFYFADRDKSFHASSPLKTKSIWDRLPPDLRLQSFQPCPIKR---------RLLLPTKIRNKPSEESQDKTRISVSRSSLFTSKSDCGKSNNFISHFKIQRPFSAKIDFCKSGMYPKETYRPPQPFDYRGLKPIKDLGLPEFTMGIEKDPGNILLNTKSLGITYGPLNEDMVFRDTTGKSMYESIKQDPKWNSQNYLPNPPWPNIPKEFTRHRVIYQDPHAAFLERVETVLTGKWKNEKLS 838
            FD  +     R K F    P+    IW R PPD R Q+++P P KR         R  L +  R + +       R+     +    K    +     + F++ RP +AK  F + G  P+E Y    P D+R   PI+ LGLPEFT  +EKDP NI   +++     GPL+E    RD +G +M+ +++Q PKW+ + +L   P+PNI  E+TR+R   ++P   F++ VE  L  +W  EKL+
Sbjct:   54 FDARQELQKTRSKHFSVPPPVPDDDIWRRPPPDFRPQAWEPRPPKRNSRDAVQPWRYRLESDARYEAAAAVAPGDRLPALGQTAAQKKQQQQQKQRMQTLFRLMRPNTAKQRFVQEGALPREPYGTVGPHDFRQYPPIQSLGLPEFTTAVEKDPNNIRFLSRNRDTIVGPLHEIKNMRDASG-TMWSAVRQPPKWDPRLHLQQNPFPNISCEYTRYRPRNRNPFDGFMDTVEAGLEKRWLAEKLA 297
[Close]
BLAST of SMED30027324 vs. TrEMBL
Match: K1Q2Z1 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10019693 PE=4 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 1.254e-32
Identity = 85/239 (35.56%), Postives = 117/239 (48.95%), Query Frame = 2 
Query:  131 FDYSKFYFADRDKSFHASSPLKTKSIWDRLPPDLRLQSFQPCPIKR---RLLLPTKIRNKPSEESQDKTRI-SVSRSSLFTSKSDCGKSNNFISHFKIQRPFSAKIDFCKSGMYPKETYRPPQPFDYRGLKPIKDLGLPEFTMGIEKDPGNILLNTKSLGITYGPLNEDMVFRDTTGKSMYESIKQDPKWNSQNYLPNPPWPNIPKEFTRHRVIYQDPHAAFLERVETVLTGKWKNEKL 835
            +D ++     R ++F    P K   IW R PPD R Q F P P KR     + P      P +    K  + +V    L  +K D G+ +     F I RPF+AK  F + GMY    ++ PQP D+RG  PIK LGL EF    EKDP +I  +T  L I +G   E    RDT G+ M      D K+++   L    WP  P E  RHR  ++  H+AF++RV+  L  +W  EK+
Sbjct:   34 YDANESLIQIRSENFSKLVPEKDNDIWKRPPPDFRPQVFAPKPPKRNSREAMRPENYGTFPGKHVTVKRPVRNVIPHMLRPAKPDEGRMS---LRFHIDRPFTAKKKFVREGMYKAGQFKNPQPHDFRGYPPIKKLGLDEFMTEYEKDPYSIHFHTGRLNIIHGLPLEKATDRDTRGRQMAPPRTPDKKYDADLILSKGSWPTKPGELNRHRPRHRPAHSAFMDRVDRTLQHQWAREKM 269
[Close]
BLAST of SMED30027324 vs. TrEMBL
Match: A0A210R3D0 (Uncharacterized protein OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT19548 PE=4 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 1.691e-32
Identity = 85/239 (35.56%), Postives = 112/239 (46.86%), Query Frame = 2 
Query:  131 FDYSKFYFADRDKSFHASSPLKTKSIWDRLPPDLRLQSFQPCPIKR---RLLLPTKIRNKPSEES-QDKTRISVSRSSLFTSKSDCGKSNNFISHFKIQRPFSAKIDFCKSGMYPKETYRPPQPFDYRGLKPIKDLGLPEFTMGIEKDPGNILLNTKSLGITYGPLNEDMVFRDTTGKSMYESIKQDPKWNSQNYLPNPPWPNIPKEFTRHRVIYQDPHAAFLERVETVLTGKWKNEKL 835
            +D ++     R  +F      K   IW R PPD R Q F P P KR     + P +    P E     K    V    L   K+  G+    I+ F I RPF+AK  F + GM+  E +  P   D+RG  PIK +GLPEFT   E DP +I  NT  L I +G   E    R+  G  M   +   P+W  Q  L N P+P  P EF RHR+ ++   +AF+ERV   L   W  E+L
Sbjct:   40 YDANESLITIRSTNFSKKELEKDDDIWKRPPPDFRPQKFAPNPPKRNSRESMQPWRYGTFPGEHVFVKKPSTKVMPKILMPKKTPEGR---IITQFHIDRPFTAKKKFVREGMFKPEMFVNPTEHDFRGYPPIKKMGLPEFTTHEEADPFDIYFNTDRLNIIHGEPLERAADRNIKGLQMAPPVHLLPRWERQLILDNEPYPTKPGEFNRHRLRFRPARSAFMERVTRTLDINWAKERL 275
[Close]
BLAST of SMED30027324 vs. TrEMBL
Match: A0A433TWZ7 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_006144 PE=4 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 1.070e-29
Identity = 83/240 (34.58%), Postives = 117/240 (48.75%), Query Frame = 2 
Query:  131 FDYSKFYFADRDKSFHASSPLKTKSIWDRLPPDLRLQSFQPCPIKRRL---LLPTKIRNKPSE-ESQDKTRISVSRSSLFTSKSDCGKSNNFISHFKIQRPFSAKIDFCKSGMYPKETYRPPQPFDYRGLKPIKDLGLPEFTMGIEKDPGNILLNTKSLGITYGPLNEDMVFRD-TTGKSMYESIKQDPKWNSQNYLPNPPWPNIPKEFTRHRVIYQDPHAAFLERVETVLTGKWKNEKL 835
            +D  +     R ++F   +  K + IW R PPD R   + P P KR     + P K    P + E   K R       LF  K    +    ++ F I RPF+AK  F + GM P  +Y  P+  D+R   P+K LGL EF    EKDP  I   +K L   +G L  D   RD   G+ M  ++    KW+SQ  L    WP   + FTR+R+ ++ P++AF+ERVE  LT KW  E++
Sbjct:   41 YDADEALLQTRSENFKLQAGEKDRDIWKRPPPDFRYVVYDPNPPKRNTVDSMQPWKFGTIPGQREIIKKNRDDGRLPRLFDQKQ---REPEIVTRFHIDRPFTAKKKFVREGMNPAGSYENPKLHDFRQYPPLKKLGLDEFITNYEKDPYGINFKSKRLNHIHG-LPSDPPERDLAEGRQMAPAMSAKKKWDSQLILDKTKWPQKNEAFTRYRLRHRQPYSAFMERVERDLTHKWSREQM 276
[Close]
BLAST of SMED30027324 vs. Ensembl Cavefish
Match: si:dkey-30e9.6 (si:dkey-30e9.6 [Source:ZFIN;Acc:ZDB-GENE-131121-308])

HSP 1 Score: 67.3958 bits (163), Expect = 8.094e-13
Identity = 64/208 (30.77%), Postives = 101/208 (48.56%), Query Frame = 2 
Query:  206 IWDRLPPDLRLQSFQPCPIKRRLLLPTKIRNKPSEESQDKTRISVSRSSLFTSKSDCGKSN--NFISHFKIQRPFSAKIDFCKSGMYPKETYRPPQPFDYRGLKPIKDLGLPEFTMGIEKDPGNILLNTKSLGITYGPLNEDMVFRDTTGKSMYESIK-QDPKWNSQNYLPNPPWPNIPKEFTRHRVIYQDPHAAFLERVETVLTGKW 820
            +W+  PPD     F P  + R L LP +I+ K  + +    R S+ ++SLF   +   K    +FI  +K      +++ F KSG YP   Y+ P+P ++R   P  D  +P+    IEKDPGN+   ++ L                  +S  ++ K  + KW+S+  LP  PWP     +TRHR   +  ++AF++RVE  LT  W
Sbjct:   47 LWNIKPPD-----FTPK-LYRSLSLP-QIKKKTLDPAI--PRESLRQTSLFLLPNLEKKMTEPSFIRSYKAPDLLESQLLFVKSGQYPNLPYKNPKPHNFR---PCAD-NMPDMVTTIEKDPGNLKFKSQYL------------------ESKIDTFKPAELKWDSKLILPKSPWPPKSASYTRHRR-RRGVYSAFMDRVEEKLTRSW 222
[Close]
BLAST of SMED30027324 vs. Ensembl Nematostella
Match: EDO49096 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RI61])

HSP 1 Score: 65.855 bits (159), Expect = 2.488e-12
Identity = 52/187 (27.81%), Postives = 82/187 (43.85%), Query Frame = 2 
Query:  191 LKTKSIWDRLPPDLRLQSFQPCPIKRRLLLPTKIRNKPSEESQDKTRISVSRSSLFTS-------KSDCGKSNNFISHFKIQRPFSAKIDFCKSGMYPKETYRPPQPFDYRG-----LKPIKDLGLPEFTMGIEKDPGNILLNTKSLGITYGPLNE-DMVFRDTTGKSMYESIKQDPKWNSQNYLPN 712
            L   ++WD  PPD RLQ +QP P+KR       ++N   E+ + +         +F         ++  GKS  F++ F++    ++KI F K G +   +Y+ P P  +RG     L P    G PEF    + DP  +   ++ L +      +  M   D   K M      DPKW++Q  LP 
Sbjct:   59 LDESTVWDIQPPDFRLQLYQPKPVKRNSRA-AMLKNYNEEDWRLQHSDRRRERVVFERVPLPKILQNGSGKSRTFVTSFRVPDSHASKIMFVKEGKFNPGSYKTPGPHMFRGDDFRPLIPPSHYGKPEFETFYKHDPQGLKAKSQGLRVLCEAYRDLQMQSPDGYQKQMISYKAPDPKWDAQLMLPQ 244
[Close]
BLAST of SMED30027324 vs. Planmine SMEST
Match: SMESG000076155.1 (SMESG000076155.1)

HSP 1 Score: 449.514 bits (1155), Expect = 9.998e-162
Identity = 219/220 (99.55%), Postives = 220/220 (100.00%), Query Frame = 2 
Query:   86 MKSCCIDRQMNDNSIFDYSKFYFADRDKSFHASSPLKTKSIWDRLPPDLRLQSFQPCPIKRRLLLPTKIRNKPSEESQDKTRISVSRSSLFTSKSDCGKSNNFISHFKIQRPFSAKIDFCKSGMYPKETYRPPQPFDYRGLKPIKDLGLPEFTMGIEKDPGNILLNTKSLGITYGPLNEDMVFRDTTGKSMYESIKQDPKWNSQNYLPNPPWPNIPKEFT 745
            MKSCCIDRQMNDNSIFDYSKFYFADRDKSFHASSPLKTKSIWDRLPPDLRLQSFQPCPIKRRLLLPTKIRNKPSEESQDKTRISVSRSSLFTSKSDCGKSNNFISHFKIQRPFSAKIDFCKSGMYPKETYRPPQPFDYRGLKPIKDLGLPEFTMGIEKDPGNILLNTKSLGITYGPLNED+VFRDTTGKSMYESIKQDPKWNSQNYLPNPPWPNIPKEFT
Sbjct:    1 MKSCCIDRQMNDNSIFDYSKFYFADRDKSFHASSPLKTKSIWDRLPPDLRLQSFQPCPIKRRLLLPTKIRNKPSEESQDKTRISVSRSSLFTSKSDCGKSNNFISHFKIQRPFSAKIDFCKSGMYPKETYRPPQPFDYRGLKPIKDLGLPEFTMGIEKDPGNILLNTKSLGITYGPLNEDIVFRDTTGKSMYESIKQDPKWNSQNYLPNPPWPNIPKEFT 220
[Close]
BLAST of SMED30027324 vs. Planmine SMEST
Match: SMESG000076155.1 (SMESG000076155.1)

HSP 1 Score: 429.869 bits (1104), Expect = 4.886e-154
Identity = 210/211 (99.53%), Postives = 211/211 (100.00%), Query Frame = 2 
Query:  113 MNDNSIFDYSKFYFADRDKSFHASSPLKTKSIWDRLPPDLRLQSFQPCPIKRRLLLPTKIRNKPSEESQDKTRISVSRSSLFTSKSDCGKSNNFISHFKIQRPFSAKIDFCKSGMYPKETYRPPQPFDYRGLKPIKDLGLPEFTMGIEKDPGNILLNTKSLGITYGPLNEDMVFRDTTGKSMYESIKQDPKWNSQNYLPNPPWPNIPKEFT 745
            MNDNSIFDYSKFYFADRDKSFHASSPLKTKSIWDRLPPDLRLQSFQPCPIKRRLLLPTKIRNKPSEESQDKTRISVSRSSLFTSKSDCGKSNNFISHFKIQRPFSAKIDFCKSGMYPKETYRPPQPFDYRGLKPIKDLGLPEFTMGIEKDPGNILLNTKSLGITYGPLNED+VFRDTTGKSMYESIKQDPKWNSQNYLPNPPWPNIPKEFT
Sbjct:    1 MNDNSIFDYSKFYFADRDKSFHASSPLKTKSIWDRLPPDLRLQSFQPCPIKRRLLLPTKIRNKPSEESQDKTRISVSRSSLFTSKSDCGKSNNFISHFKIQRPFSAKIDFCKSGMYPKETYRPPQPFDYRGLKPIKDLGLPEFTMGIEKDPGNILLNTKSLGITYGPLNEDIVFRDTTGKSMYESIKQDPKWNSQNYLPNPPWPNIPKEFT 211
The following BLAST results are available for this feature:
BLAST of SMED30027324 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
AC013470.22.255e-1427.62novel protein[more]
AC013470.26.959e-1427.62novel protein[more]
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BLAST of SMED30027324 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027324 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027324 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
si:dkey-30e9.61.076e-1029.53si:dkey-30e9.6 [Source:ZFIN;Acc:ZDB-GENE-131121-30... [more]
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BLAST of SMED30027324 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027324 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027324 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027324 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267ERK94.424e-3333.47Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267G1E76.879e-3333.06Uncharacterized protein OS=Macrostomum lignano OX=... [more]
K1Q2Z11.254e-3235.56Uncharacterized protein OS=Crassostrea gigas OX=29... [more]
A0A210R3D01.691e-3235.56Uncharacterized protein OS=Mizuhopecten yessoensis... [more]
A0A433TWZ71.070e-2934.58Uncharacterized protein OS=Elysia chlorotica OX=18... [more]
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BLAST of SMED30027324 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
si:dkey-30e9.68.094e-1330.77si:dkey-30e9.6 [Source:ZFIN;Acc:ZDB-GENE-131121-30... [more]
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BLAST of SMED30027324 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027324 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027324 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO490962.488e-1227.81Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30027324 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027324 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000076155.19.998e-16299.55SMESG000076155.1[more]
SMESG000076155.14.886e-15499.53SMESG000076155.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027324 ID=SMED30027324|Name=SMED30027324|organism=Schmidtea mediterranea sexual|type=transcript|length=920bp
TTGCTATAGGTAACTATCGTTAACAATTAAACAAAACAAAATCATCAAAA
TACCAGAAAAATATAACTTTTTCACTAAATTTCCAATGAAATCTTGCTGC
ATTGACAGACAAATGAACGACAATTCAATTTTCGACTATTCCAAATTCTA
CTTTGCTGATAGAGACAAATCTTTCCATGCATCAAGTCCTTTGAAAACGA
AATCTATCTGGGATAGACTGCCTCCGGATTTACGTTTGCAAAGCTTTCAA
CCTTGTCCTATTAAACGTAGATTGTTGTTGCCAACAAAAATCCGAAACAA
ACCTTCAGAAGAATCACAAGACAAAACAAGGATTTCTGTGTCTAGGAGTT
CATTGTTTACGAGCAAATCTGATTGCGGAAAAAGTAACAATTTTATTTCC
CATTTCAAAATCCAAAGACCATTTAGTGCAAAAATCGATTTCTGTAAATC
TGGAATGTATCCAAAGGAAACTTACCGACCTCCACAGCCATTTGATTATA
GAGGGTTGAAACCGATTAAGGACTTGGGACTTCCTGAATTCACAATGGGC
ATTGAAAAAGATCCTGGAAATATTTTGTTAAACACAAAATCTTTGGGAAT
AACTTACGGACCCTTGAATGAAGACATGGTATTCAGAGACACGACTGGCA
AAAGTATGTATGAATCTATAAAACAAGATCCAAAATGGAATTCACAAAAT
TATCTCCCAAATCCTCCATGGCCTAATATACCGAAAGAGTTTACGCGTCA
TAGGGTAATTTATCAAGATCCTCATGCGGCATTCCTTGAGCGGGTGGAAA
CTGTTTTGACAGGAAAATGGAAAAATGAGAAGTTAAGTGCATGATACAGA
AATTGGAAAATAGTCAGCTTATTAGCAATGTCGCAATATAATGATGTAGT
TCAAAATATAAAGACTGTGC
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protein sequence of SMED30027324-orf-1

>SMED30027324-orf-1 ID=SMED30027324-orf-1|Name=SMED30027324-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=253bp
MKSCCIDRQMNDNSIFDYSKFYFADRDKSFHASSPLKTKSIWDRLPPDLR
LQSFQPCPIKRRLLLPTKIRNKPSEESQDKTRISVSRSSLFTSKSDCGKS
NNFISHFKIQRPFSAKIDFCKSGMYPKETYRPPQPFDYRGLKPIKDLGLP
EFTMGIEKDPGNILLNTKSLGITYGPLNEDMVFRDTTGKSMYESIKQDPK
WNSQNYLPNPPWPNIPKEFTRHRVIYQDPHAAFLERVETVLTGKWKNEKL
SA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000054dorsal region of the epidermis
PLANA:0000136whole organism
PLANA:0000418head