X1.A.B7.1
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30027307 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 21Note: Hover over icons to view figure legend
Homology
BLAST of X1.A.B7.1 vs. TrEMBL
Match: V9XRM1 (X1.A.B7.1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 253.832 bits (647), Expect = 2.737e-82 Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 2 Query: 551 MKISIILLVCGLAIAAVNVKVDAIPTEMNEFPEISRVEYDNIMAKISTLLPADVFQKVQGYATQLAGLYKQVKEAWSDPARKDEFNGLFEKVMAIVKPLMEIKDKYLPDLNLDDLFDADSNVESR 925 MKISIILLVCGLAIAAVNVKVDAIPTEMNEFPEISRVEYDNIMAKISTLLPADVFQKVQGYATQLAGLYKQVKEAWSDPARKDEFNGLFEKVMAIVKPLMEIKDKYLPDLNLDDLFDADSNVESR Sbjct: 1 MKISIILLVCGLAIAAVNVKVDAIPTEMNEFPEISRVEYDNIMAKISTLLPADVFQKVQGYATQLAGLYKQVKEAWSDPARKDEFNGLFEKVMAIVKPLMEIKDKYLPDLNLDDLFDADSNVESR 125
BLAST of X1.A.B7.1 vs. Planmine SMEST
Match: SMESG000048209.1 (SMESG000048209.1) HSP 1 Score: 260.766 bits (665), Expect = 2.169e-88 Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 2 Query: 542 MSNMKISIILLVCGLAIAAVNVKVDAIPTEMNEFPEISRVEYDNIMAKISTLLPADVFQKVQGYATQLAGLYKQVKEAWSDPARKDEFNGLFEKVMAIVKPLMEIKDKYLPDLNLDDLFDADSNVESR 925 MSNMKISIILLVCGLAIAAVNVKVDAIPTEMNEFPEISRVEYDNIMAKISTLLPADVFQKVQGYATQLAGLYKQVKEAWSDPARKDEFNGLFEKVMAIVKPLMEIKDKYLPDLNLDDLFDADSNVESR Sbjct: 1 MSNMKISIILLVCGLAIAAVNVKVDAIPTEMNEFPEISRVEYDNIMAKISTLLPADVFQKVQGYATQLAGLYKQVKEAWSDPARKDEFNGLFEKVMAIVKPLMEIKDKYLPDLNLDDLFDADSNVESR 128
BLAST of X1.A.B7.1 vs. Planmine SMEST
Match: SMESG000048204.1 (SMESG000048204.1) HSP 1 Score: 258.84 bits (660), Expect = 1.022e-87 Identity = 127/128 (99.22%), Postives = 127/128 (99.22%), Query Frame = 2 Query: 542 MSNMKISIILLVCGLAIAAVNVKVDAIPTEMNEFPEISRVEYDNIMAKISTLLPADVFQKVQGYATQLAGLYKQVKEAWSDPARKDEFNGLFEKVMAIVKPLMEIKDKYLPDLNLDDLFDADSNVESR 925 MSNMKISIILLVCGLAIAAVNVKVDAIPTEMNEFPEISRVEYDN MAKISTLLPADVFQKVQGYATQLAGLYKQVKEAWSDPARKDEFNGLFEKVMAIVKPLMEIKDKYLPDLNLDDLFDADSNVESR Sbjct: 1 MSNMKISIILLVCGLAIAAVNVKVDAIPTEMNEFPEISRVEYDNFMAKISTLLPADVFQKVQGYATQLAGLYKQVKEAWSDPARKDEFNGLFEKVMAIVKPLMEIKDKYLPDLNLDDLFDADSNVESR 128
BLAST of X1.A.B7.1 vs. Planmine SMEST
Match: SMESG000048212.1 (SMESG000048212.1) HSP 1 Score: 254.603 bits (649), Expect = 4.371e-86 Identity = 125/128 (97.66%), Postives = 127/128 (99.22%), Query Frame = 2 Query: 542 MSNMKISIILLVCGLAIAAVNVKVDAIPTEMNEFPEISRVEYDNIMAKISTLLPADVFQKVQGYATQLAGLYKQVKEAWSDPARKDEFNGLFEKVMAIVKPLMEIKDKYLPDLNLDDLFDADSNVESR 925 MSNMKISIILLVCGLAIAAVNVKVDAIPT M++FPEISRVEYDNIMAKISTLLPADVFQKVQGYATQLAGLYKQVKEAWSDPARKDEFNGLFEKVMAIVKPLMEIKDKYLPDLNLDDLFDADSNVESR Sbjct: 1 MSNMKISIILLVCGLAIAAVNVKVDAIPTGMDQFPEISRVEYDNIMAKISTLLPADVFQKVQGYATQLAGLYKQVKEAWSDPARKDEFNGLFEKVMAIVKPLMEIKDKYLPDLNLDDLFDADSNVESR 128
BLAST of X1.A.B7.1 vs. Planmine SMEST
Match: SMESG000048213.1 (SMESG000048213.1) HSP 1 Score: 253.062 bits (645), Expect = 1.748e-85 Identity = 124/128 (96.88%), Postives = 126/128 (98.44%), Query Frame = 2 Query: 542 MSNMKISIILLVCGLAIAAVNVKVDAIPTEMNEFPEISRVEYDNIMAKISTLLPADVFQKVQGYATQLAGLYKQVKEAWSDPARKDEFNGLFEKVMAIVKPLMEIKDKYLPDLNLDDLFDADSNVESR 925 MSNMKISIILLVCGLAIAAVNVKVDAIPT M++FPEISRVEYDN MAKISTLLPADVFQKVQGYATQLAGLYKQVKEAWSDPARKDEFNGLFEKVMAIVKPLMEIKDKYLPDLNLDDLFDADSNVESR Sbjct: 1 MSNMKISIILLVCGLAIAAVNVKVDAIPTGMDQFPEISRVEYDNFMAKISTLLPADVFQKVQGYATQLAGLYKQVKEAWSDPARKDEFNGLFEKVMAIVKPLMEIKDKYLPDLNLDDLFDADSNVESR 128
BLAST of X1.A.B7.1 vs. Planmine SMEST
Match: SMESG000048206.1 (SMESG000048206.1) HSP 1 Score: 253.062 bits (645), Expect = 1.748e-85 Identity = 124/128 (96.88%), Postives = 126/128 (98.44%), Query Frame = 2 Query: 542 MSNMKISIILLVCGLAIAAVNVKVDAIPTEMNEFPEISRVEYDNIMAKISTLLPADVFQKVQGYATQLAGLYKQVKEAWSDPARKDEFNGLFEKVMAIVKPLMEIKDKYLPDLNLDDLFDADSNVESR 925 MSNMKISIILLVCGLAIAAVNVKVDAIPT M++FPEISRVEYDN MAKISTLLPADVFQKVQGYATQLAGLYKQVKEAWSDPARKDEFNGLFEKVMAIVKPLMEIKDKYLPDLNLDDLFDADSNVESR Sbjct: 1 MSNMKISIILLVCGLAIAAVNVKVDAIPTGMDQFPEISRVEYDNFMAKISTLLPADVFQKVQGYATQLAGLYKQVKEAWSDPARKDEFNGLFEKVMAIVKPLMEIKDKYLPDLNLDDLFDADSNVESR 128 The following BLAST results are available for this feature:
BLAST of X1.A.B7.1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of X1.A.B7.1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of X1.A.B7.1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of X1.A.B7.1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of X1.A.B7.1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of X1.A.B7.1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of X1.A.B7.1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of X1.A.B7.1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 1
BLAST of X1.A.B7.1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of X1.A.B7.1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of X1.A.B7.1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of X1.A.B7.1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of X1.A.B7.1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of X1.A.B7.1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Cross References
External references for this transcript
Sequences
The following sequences are available for this feature:
transcript sequence >SMED30027307 ID=SMED30027307|Name=X1.A.B7.1|organism=Schmidtea mediterranea sexual|type=transcript|length=947bpback to top protein sequence of SMED30027307-orf-1 >SMED30027307-orf-1 ID=SMED30027307-orf-1|Name=SMED30027307-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=129bp MSNMKISIILLVCGLAIAAVNVKVDAIPTEMNEFPEISRVEYDNIMAKISback to top protein sequence of SMED30027307-orf-2 >SMED30027307-orf-2 ID=SMED30027307-orf-2|Name=SMED30027307-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=100bp MTRSGHHPKTTRTCTSTRNMRVASTWFIDFQLNILATIFRFWIYLCSFSIback to top protein sequence of SMED30027307-orf-3 >SMED30027307-orf-3 ID=SMED30027307-orf-3|Name=SMED30027307-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=114bp MLSYSTRLISGNSFISVGIASTLTLTAAMARPQTSKMIEIFMFDILYDFTback to top Aliases
The feature 'X1.A.B7.1' has the following synonyms
Annotated Terms
The following terms have been associated with this transcript:
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