Ast1 protein

Overview
NameAst1 protein (CCW36839.1)
Smed IDSMED30027262
Length (bp)805
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30027262

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30027262h1SMcG0000570 SmedASXL_009672SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30027262h1SMcG0000570 SmedASXL_009672SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30027262h1SMcG0000570 dd_Smed_v6_51_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30027262h1SMcG0000570 dd_Smed_v6_85_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30027262h1SMcG0000570 OX_Smed_1.0.03748ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Ast1 protein vs. Ensembl Human
Match: ASTL (astacin like metalloendopeptidase [Source:HGNC Symbol;Acc:HGNC:31704])

HSP 1 Score: 83.5741 bits (205), Expect = 1.062e-17
Identity = 56/179 (31.28%), Postives = 83/179 (46.37%), Query Frame = 2 
Query:  125 LLDKSDIALINGFKNHYANTKKWPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKF-TESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMY-SKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFA 640
             L + DI   + F+   A + KWP        V +  +  +      V+ + L E +R    C++F T  +Q  +I +     GC+S +G + GM  Q ++L  P C+   +G V HE +H LGF H H R DRD +I +NW  I       F K        PY+  SVMHY    F+
Sbjct:   72 FLIEGDIIRPSPFRLLSATSNKWPMGGSGVVEVPFLLSSKYDEPSRQVILEALAEFERS--TCIRFVTYQDQRDFISII-PMYGCFSSVGRSGGM--QVVSLA-PTCLQKGRGIVLHELMHVLGFWHEHTRADRDRYIRVNWNEILPGFEINFIKSQSSNMLTPYDYSSVMHYGRLAFS 244
[Close]
BLAST of Ast1 protein vs. Ensembl Human
Match: TLL1 (tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843])

HSP 1 Score: 70.0922 bits (170), Expect = 4.362e-13
Identity = 48/156 (30.77%), Postives = 73/156 (46.79%), Query Frame = 2 
Query:  185 KKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDG---PYETESVMHYNSEGFA 640
            + WP   + Y   GNF   +  + +Q +   ++    CV F E S++  YI    +  GC S +G     P Q +++      +  G V HE  H +GF H H R DRDNH+TI    I+  Q   F K +  E +     Y+ +S+MHY    F+
Sbjct:  155 RIWPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKH--TCVTFIERSDEESYIVFTYRPCGCCSYVGRRGNGP-QAISIGKNCDKF--GIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFS 305
[Close]
BLAST of Ast1 protein vs. Ensembl Human
Match: BMP1 (bone morphogenetic protein 1 [Source:HGNC Symbol;Acc:HGNC:1067])

HSP 1 Score: 70.0922 bits (170), Expect = 5.560e-13
Identity = 47/156 (30.13%), Postives = 74/156 (47.44%), Query Frame = 2 
Query:  185 KKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDG---PYETESVMHYNSEGFA 640
            + WP+  + +   GNF   +  V RQ +   ++    CV F E +++  YI    +  GC S +G   G P Q +++      +  G V HE  H +GF H H R DRD H++I    I+  Q   F K +  E +     Y+ +S+MHY    F+
Sbjct:  128 RVWPDGVIPFVIGGNFTGSQRAVFRQAMRHWEKH--TCVTFLERTDEDSYIVFTYRPCGCCSYVGRRGGGP-QAISIGKNCDKF--GIVVHELGHVVGFWHEHTRPDRDRHVSIVRENIQPGQEYNFLKMEPQEVESLGETYDFDSIMHYARNTFS 278
[Close]
BLAST of Ast1 protein vs. Ensembl Human
Match: BMP1 (bone morphogenetic protein 1 [Source:HGNC Symbol;Acc:HGNC:1067])

HSP 1 Score: 69.707 bits (169), Expect = 6.543e-13
Identity = 47/156 (30.13%), Postives = 74/156 (47.44%), Query Frame = 2 
Query:  185 KKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDG---PYETESVMHYNSEGFA 640
            + WP+  + +   GNF   +  V RQ +   ++    CV F E +++  YI    +  GC S +G   G P Q +++      +  G V HE  H +GF H H R DRD H++I    I+  Q   F K +  E +     Y+ +S+MHY    F+
Sbjct:  128 RVWPDGVIPFVIGGNFTGSQRAVFRQAMRHWEKH--TCVTFLERTDEDSYIVFTYRPCGCCSYVGRRGGGP-QAISIGKNCDKF--GIVVHELGHVVGFWHEHTRPDRDRHVSIVRENIQPGQEYNFLKMEPQEVESLGETYDFDSIMHYARNTFS 278
[Close]
BLAST of Ast1 protein vs. Ensembl Human
Match: BMP1 (bone morphogenetic protein 1 [Source:HGNC Symbol;Acc:HGNC:1067])

HSP 1 Score: 69.707 bits (169), Expect = 7.676e-13
Identity = 47/156 (30.13%), Postives = 74/156 (47.44%), Query Frame = 2 
Query:  185 KKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDG---PYETESVMHYNSEGFA 640
            + WP+  + +   GNF   +  V RQ +   ++    CV F E +++  YI    +  GC S +G   G P Q +++      +  G V HE  H +GF H H R DRD H++I    I+  Q   F K +  E +     Y+ +S+MHY    F+
Sbjct:  128 RVWPDGVIPFVIGGNFTGSQRAVFRQAMRHWEKH--TCVTFLERTDEDSYIVFTYRPCGCCSYVGRRGGGP-QAISIGKNCDKF--GIVVHELGHVVGFWHEHTRPDRDRHVSIVRENIQPGQEYNFLKMEPQEVESLGETYDFDSIMHYARNTFS 278
[Close]
BLAST of Ast1 protein vs. Ensembl Celegans
Match: nas-4 (Zinc metalloproteinase nas-4 [Source:UniProtKB/Swiss-Prot;Acc:P55112])

HSP 1 Score: 93.5893 bits (231), Expect = 3.773e-22
Identity = 56/171 (32.75%), Postives = 89/171 (52.05%), Query Frame = 2 
Query:  185 KKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE---SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCD---GCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNG 679
            ++WPNN + Y+ +  +      V+   + E       CVKF     S    Y+ ++   EGCYSL+G   G   QP++L++ GC+   GT+ HE +HA+GF H   R+DRD++I + W  +      QF K +       D PY+  S+MHY    F+ +  + +V KK+G
Sbjct:  102 RRWPNNEIPYTLSSQYGSYARSVIANAMNEYHTK--TCVKFVARDPSKHHDYLWIHP-DEGCYSLVGKTGG--KQPVSLDS-GCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSGSGKKTLVPKKSG 265
[Close]
BLAST of Ast1 protein vs. Ensembl Celegans
Match: nas-4 (Zinc metalloproteinase nas-4 [Source:UniProtKB/Swiss-Prot;Acc:P55112])

HSP 1 Score: 92.8189 bits (229), Expect = 1.420e-21
Identity = 56/172 (32.56%), Postives = 89/172 (51.74%), Query Frame = 2 
Query:  185 KKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE---SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCD---GCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGG 682
            ++WPNN + Y+ +  +      V+   + E       CVKF     S    Y+ ++   EGCYSL+G   G   QP++L++ GC+   GT+ HE +HA+GF H   R+DRD++I + W  +      QF K +       D PY+  S+MHY    F+ +  + +V KK+G 
Sbjct:  102 RRWPNNEIPYTLSSQYGSYARSVIANAMNEYH--TKTCVKFVARDPSKHHDYLWIHP-DEGCYSLVGKTGG--KQPVSLDS-GCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSGSGKKTLVPKKSGS 266
[Close]
BLAST of Ast1 protein vs. Ensembl Celegans
Match: nas-4 (Zinc metalloproteinase nas-4 [Source:UniProtKB/Swiss-Prot;Acc:P55112])

HSP 1 Score: 92.4337 bits (228), Expect = 1.659e-21
Identity = 56/172 (32.56%), Postives = 89/172 (51.74%), Query Frame = 2 
Query:  185 KKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE---SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCD---GCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGG 682
            ++WPNN + Y+ +  +      V+   + E       CVKF     S    Y+ ++   EGCYSL+G   G   QP++L++ GC+   GT+ HE +HA+GF H   R+DRD++I + W  +      QF K +       D PY+  S+MHY    F+ +  + +V KK+G 
Sbjct:  102 RRWPNNEIPYTLSSQYGSYARSVIANAMNEYH--TKTCVKFVARDPSKHHDYLWIHP-DEGCYSLVGKTGG--KQPVSLDS-GCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSGSGKKTLVPKKSGS 266
[Close]
BLAST of Ast1 protein vs. Ensembl Celegans
Match: nas-8 (Zinc metalloproteinase nas-8 [Source:UniProtKB/Swiss-Prot;Acc:Q18439])

HSP 1 Score: 91.2781 bits (225), Expect = 7.896e-21
Identity = 63/184 (34.24%), Postives = 92/184 (50.00%), Query Frame = 2 
Query:  182 TKKWPNNNVVYSFAGNF-PRDKIPVVRQCLGELQRDLGNCVKFTESN--QGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPD---GPYETESVMHYNSEGFACNPSEKVVVKKN------GGAIGYN 697
            T+KWPN  + Y  +  +  R++  + R       +    CV+F         Y+ +  K +GCYS +G A G   Q L+L+N GC+    T  HE +H++GF H H R DRD HITI W  I+     QF K D  E       Y+  S+MHY+S  F+ N  E +V K++      G AI ++
Sbjct:  118 TRKWPNGRIPYVISNQYNDRERAVLARSFQAYHDK---TCVRFVPRTAVDNDYLYIG-KIDGCYSDVGRAGGR--QELSLDN-GCL-QYDTAIHELMHSVGFYHEHERWDRDEHITILWHNIDREAYDQFGKVDLAESSYYGQLYDYYSIMHYDSLAFSKNGFETMVAKQSEMTAVIGAAIDFS 293
[Close]
BLAST of Ast1 protein vs. Ensembl Celegans
Match: nas-7 (Zinc metalloproteinase nas-7 [Source:UniProtKB/Swiss-Prot;Acc:P55113])

HSP 1 Score: 90.5077 bits (223), Expect = 1.285e-20
Identity = 64/223 (28.70%), Postives = 109/223 (48.88%), Query Frame = 2 
Query:   74 RQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYAN--TKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGH--YIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPD---GPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDL 721
            R++L  + +    + D    KSDI L    K +  +   K WPN  + Y+ + ++   +  ++ + + +       C++F     G   Y+ +  K +GC+S +G  +G+  Q L+L+N GCM    T+ HE +H +GF H H R DRDN I I W  I+     QF K D  +      PY+ +S++HY+S  F+ N    ++ K     IG   + +  D+
Sbjct:   51 REELFGKHIPVEVVNDF---KSDIRLPRRHKRNGVSRAAKLWPNARIPYAISPHYSPHERALLAKAVKQYHE--KTCIRFVPRQTGEPDYLFIG-KVDGCFSEVGRTSGV--QVLSLDN-GCM-EYATIIHEMMHVVGFYHEHERWDRDNFIDIIWQNIDRGALDQFGKVDLSKTSYYGQPYDYKSILHYDSLAFSKNGFPTMLPKVKSATIGNARDFSDVDI 263
[Close]
BLAST of Ast1 protein vs. Ensembl Fly
Match: CG6763 (gene:FBgn0039069 transcript:FBtr0084378)

HSP 1 Score: 124.79 bits (312), Expect = 2.884e-33
Identity = 64/192 (33.33%), Postives = 102/192 (53.12%), Query Frame = 2 
Query:  116 GDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDG-PYETESVMHYNSEGFACNPSEKVVVKKNGGA 685
            GDML+ ++D+ + NG       + +WPN  V Y   GNF    +  +   +GE  R    C++F + S++  YI +     GC+S +G   G   Q +NL++PGC+   GT  HE +HALGF+H   R +RD ++ I +  +++S  + F K    E  G PY+  SVMHY+   F+ N    ++  +  GA
Sbjct:   95 GDMLVPQTDLIMKNGLPTQ---SSRWPNGVVPYEIRGNFNARDMATIENAIGEYHRR--TCIRFVKRSSERDYISIRGDNSGCWSSVGRVGG--KQEVNLQSPGCLSRPGTAMHELMHALGFLHEQNRMERDGYVAIQYNNVQSSAMNNFEKAARTEAFGVPYDYGSVMHYSKNAFSINGQPTILAMQANGA 279
[Close]
BLAST of Ast1 protein vs. Ensembl Fly
Match: CG6763 (gene:FBgn0039069 transcript:FBtr0334497)

HSP 1 Score: 124.79 bits (312), Expect = 2.884e-33
Identity = 64/192 (33.33%), Postives = 102/192 (53.12%), Query Frame = 2 
Query:  116 GDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDG-PYETESVMHYNSEGFACNPSEKVVVKKNGGA 685
            GDML+ ++D+ + NG       + +WPN  V Y   GNF    +  +   +GE  R    C++F + S++  YI +     GC+S +G   G   Q +NL++PGC+   GT  HE +HALGF+H   R +RD ++ I +  +++S  + F K    E  G PY+  SVMHY+   F+ N    ++  +  GA
Sbjct:   95 GDMLVPQTDLIMKNGLPTQ---SSRWPNGVVPYEIRGNFNARDMATIENAIGEYHRR--TCIRFVKRSSERDYISIRGDNSGCWSSVGRVGG--KQEVNLQSPGCLSRPGTAMHELMHALGFLHEQNRMERDGYVAIQYNNVQSSAMNNFEKAARTEAFGVPYDYGSVMHYSKNAFSINGQPTILAMQANGA 279
[Close]
BLAST of Ast1 protein vs. Ensembl Fly
Match: CG11865 (gene:FBgn0028947 transcript:FBtr0080753)

HSP 1 Score: 92.0485 bits (227), Expect = 5.457e-22
Identity = 60/183 (32.79%), Postives = 88/183 (48.09%), Query Frame = 2 
Query:  191 WPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKF--TESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFT--KCDGCEPDG-PYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQ 724
            W    +VYS+AG F    I  +   + E+      CVKF  TE  +   + +  +  GC+S +GY  G   Q LNL   GCM S  T++HE +HALGF HTH    RD ++ I    I +     F   + +G    G  Y+ +S+MHY    F+ N    +V  K+   IG   + +  D+Q
Sbjct:   56 WKGRTLVYSYAGGFSSLDIASIESAMAEISSK--TCVKFRRTEYKREPQVVIQKEGSGCWSYVGYL-GRADQTLNL-GSGCM-SNRTIQHELLHALGFFHTHSDPQRDKYVRIQTDNIRSGHEHNFQRLRANGVTNYGFGYDYDSIMHYGPFAFSKNGQSTIVPLKSHAKIGQATQMSPKDVQ 233
[Close]
BLAST of Ast1 protein vs. Ensembl Fly
Match: CG15255 (gene:FBgn0028950 transcript:FBtr0080785)

HSP 1 Score: 85.1149 bits (209), Expect = 3.292e-19
Identity = 61/196 (31.12%), Postives = 94/196 (47.96%), Query Frame = 2 
Query:  164 KNHYANT-KKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGH--YIEVNSKQEGCYSLLGYAAGMPTQPLNLE----NPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTK-CDGCEPDGP--YETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDL 721
            +N   NT K+WP N VVY  + +F       ++  +  L+  L  C++F E+      Y+ V +K  GCY+ +GY  G P Q +NLE      GC +  GT+ HEF+HALGF H      RD+ I + +  I   +   F K  D    D    Y+ +S +HY    F+ N  + +V   +   IG     ++ D+
Sbjct:   58 RNGLINTEKRWPGNVVVYRISDDFDTAHKKAIQTGIDTLE--LHTCLRFREATDEDKAYLTVTAKSGGCYTAVGYQ-GAP-QEMNLEIYPLGEGC-FRPGTILHEFMHALGFYHQQSSSIRDDFINVIYENIVPGKEFNFQKYADTVVTDFEVGYDYDSCLHYRPGAFSINGEDTIVPLDSSAVIGQRVGLSSKDI 248
[Close]
BLAST of Ast1 protein vs. Ensembl Fly
Match: CG15255 (gene:FBgn0028950 transcript:FBtr0343719)

HSP 1 Score: 85.1149 bits (209), Expect = 3.292e-19
Identity = 61/196 (31.12%), Postives = 94/196 (47.96%), Query Frame = 2 
Query:  164 KNHYANT-KKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGH--YIEVNSKQEGCYSLLGYAAGMPTQPLNLE----NPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTK-CDGCEPDGP--YETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDL 721
            +N   NT K+WP N VVY  + +F       ++  +  L+  L  C++F E+      Y+ V +K  GCY+ +GY  G P Q +NLE      GC +  GT+ HEF+HALGF H      RD+ I + +  I   +   F K  D    D    Y+ +S +HY    F+ N  + +V   +   IG     ++ D+
Sbjct:   58 RNGLINTEKRWPGNVVVYRISDDFDTAHKKAIQTGIDTLE--LHTCLRFREATDEDKAYLTVTAKSGGCYTAVGYQ-GAP-QEMNLEIYPLGEGC-FRPGTILHEFMHALGFYHQQSSSIRDDFINVIYENIVPGKEFNFQKYADTVVTDFEVGYDYDSCLHYRPGAFSINGEDTIVPLDSSAVIGQRVGLSSKDI 248
[Close]
BLAST of Ast1 protein vs. Ensembl Zebrafish
Match: hce2l2 (high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:ZDB-GENE-070912-146])

HSP 1 Score: 91.2781 bits (225), Expect = 7.377e-22
Identity = 60/190 (31.58%), Postives = 98/190 (51.58%), Query Frame = 2 
Query:  128 LDKSDIALI-NGFKNHYANTKKWP-----NNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKN 676
            L + DIA+     KN +A +  W      N  + YS +  +    +  +++ +  +++D   CV+F   ++Q  Y+++  K  GC+S LG   G   Q ++L++P C  S  TV HE +HALGF+H   R DRD ++TI W+ I   +   F K      D PY+  SVMH+    F+ +    +V K+N
Sbjct:    6 LREGDIAVSGRSQKNCFARSCLWTKSVDGNVYIAYSLSHAYDDADVKNIKEGMELIEQD--TCVRFVPRTHQRDYLDIQPKT-GCWSYLGARGGR--QTISLQSPDCTGSGVTV-HELMHALGFVHEQSRADRDKYVTIMWSNIWKDRLRNFEKFKTNNLDTPYDYSSVMHFGKYAFSEDGEPTIVPKRN 189
[Close]
BLAST of Ast1 protein vs. Ensembl Zebrafish
Match: c6ast3 (six-cysteine containing astacin protease 3 [Source:ZFIN;Acc:ZDB-GENE-050320-99])

HSP 1 Score: 92.4337 bits (228), Expect = 7.433e-22
Identity = 57/184 (30.98%), Postives = 88/184 (47.83%), Query Frame = 2 
Query:  191 WPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKF-TESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVV-VKKNGGAIGYNHETTANDL 721
            WP  +     V Y  A ++   ++ ++++ L         C++F    N+  YI + S+  GCYS +G       Q ++L   GC+Y   TV+HE +HALGF H   R DRDNHI + W  I       F K +      PY+  SVM Y    F+ N    ++ +  N  A+G + E + ND+
Sbjct:   69 WPKYSDGKIYVPYVIANHYSSRELEIIQRGLDSFS--YSTCIRFFPRGNERDYISIESR-SGCYSYVGRQGY--AQTVSLARSGCLY-HSTVQHELLHALGFNHEQTRNDRDNHIQVIWENILDDMKYNFNKINTLNQGTPYDYSSVMQYERYAFSKNGLPTMIPIPNNNAALGTSTEMSQNDI 246
[Close]
BLAST of Ast1 protein vs. Ensembl Zebrafish
Match: c6ast3 (six-cysteine containing astacin protease 3 [Source:ZFIN;Acc:ZDB-GENE-050320-99])

HSP 1 Score: 92.4337 bits (228), Expect = 7.433e-22
Identity = 57/184 (30.98%), Postives = 88/184 (47.83%), Query Frame = 2 
Query:  191 WPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKF-TESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVV-VKKNGGAIGYNHETTANDL 721
            WP  +     V Y  A ++   ++ ++++ L         C++F    N+  YI + S+  GCYS +G       Q ++L   GC+Y   TV+HE +HALGF H   R DRDNHI + W  I       F K +      PY+  SVM Y    F+ N    ++ +  N  A+G + E + ND+
Sbjct:   69 WPKYSDGKIYVPYVIANHYSSRELEIIQRGLDSFS--YSTCIRFFPRGNERDYISIESR-SGCYSYVGRQGY--AQTVSLARSGCLY-HSTVQHELLHALGFNHEQTRNDRDNHIQVIWENILDDMKYNFNKINTLNQGTPYDYSSVMQYERYAFSKNGLPTMIPIPNNNAALGTSTEMSQNDI 246
[Close]
BLAST of Ast1 protein vs. Ensembl Zebrafish
Match: c6ast4 (six-cysteine containing astacin protease 4 [Source:ZFIN;Acc:ZDB-GENE-050626-100])

HSP 1 Score: 92.4337 bits (228), Expect = 7.782e-22
Identity = 56/184 (30.43%), Postives = 91/184 (49.46%), Query Frame = 2 
Query:  191 WPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKF-TESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVV-VKKNGGAIGYNHETTANDL 721
            WP  +     V Y  A ++   ++ ++++ L        +C++F   SN+  YI + S+  GCYS +G    +  Q ++L   GC+Y   TV+HE +HALGF H   R DRDNHI + W  I       F K +      PY+ +SVM Y    F+ N    ++ +  N   +G + + + ND+
Sbjct:   79 WPKYSDGKIYVPYVIANHYSSRELEIIQRGLDSFAS--VSCIRFFRRSNERDYISIESRS-GCYSYVGRQGNV--QTVSLARSGCLY-HSTVQHELLHALGFNHEQTRSDRDNHIQVIWENILDDMKYNFNKINTLNQGTPYDYKSVMQYERYAFSKNGYPTMIPIPNNNAELGKSTQMSQNDI 256
[Close]
BLAST of Ast1 protein vs. Ensembl Zebrafish
Match: c6ast4 (six-cysteine containing astacin protease 4 [Source:ZFIN;Acc:ZDB-GENE-050626-100])

HSP 1 Score: 92.4337 bits (228), Expect = 7.782e-22
Identity = 56/184 (30.43%), Postives = 91/184 (49.46%), Query Frame = 2 
Query:  191 WPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKF-TESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVV-VKKNGGAIGYNHETTANDL 721
            WP  +     V Y  A ++   ++ ++++ L        +C++F   SN+  YI + S+  GCYS +G    +  Q ++L   GC+Y   TV+HE +HALGF H   R DRDNHI + W  I       F K +      PY+ +SVM Y    F+ N    ++ +  N   +G + + + ND+
Sbjct:   79 WPKYSDGKIYVPYVIANHYSSRELEIIQRGLDSFAS--VSCIRFFRRSNERDYISIESRS-GCYSYVGRQGNV--QTVSLARSGCLY-HSTVQHELLHALGFNHEQTRSDRDNHIQVIWENILDDMKYNFNKINTLNQGTPYDYKSVMQYERYAFSKNGYPTMIPIPNNNAELGKSTQMSQNDI 256
[Close]
BLAST of Ast1 protein vs. Ensembl Xenopus
Match: ENSXETT00000022029.1 (astacin-like metalloendopeptidase [Source:NCBI gene;Acc:100496745])

HSP 1 Score: 91.2781 bits (225), Expect = 1.924e-20
Identity = 60/195 (30.77%), Postives = 97/195 (49.74%), Query Frame = 2 
Query:  107 MKMGDMLLDKSDIALINGFKNHYANTKKWPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKF-TESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKK 673
            M  GD+ + KS  AL+   K     +  WP +      V Y+ + N+   +  ++R  + E+   +  C++F T +N+  Y+ +    +GC+S +G   G   Q ++L   GC++  G ++HE +H+LGF H   R DRDN+I INW  I   +   F K +      PY+  SVMHY    FA N  +  +  K
Sbjct:    1 MIQGDIAVKKSRNALMCPGK-----SCLWPRSRKGLVLVPYTLSNNYNSTERDIIRAAMDEVT--VLTCIQFVTYNNESDYLRIR-PYDGCWSYIGRVGG--AQDVSLMKTGCLH-HGVIQHELLHSLGFQHEQCRSDRDNYININWDNISHDKERNFLKMNTQNLGSPYDYLSVMHYGKFAFATNSGKPTLEPK 184
[Close]
BLAST of Ast1 protein vs. Ensembl Xenopus
Match: kpna4 (karyopherin alpha 4 (importin alpha 3) [Source:Xenbase;Acc:XB-GENE-1011058])

HSP 1 Score: 89.7373 bits (221), Expect = 7.813e-20
Identity = 62/197 (31.47%), Postives = 102/197 (51.78%), Query Frame = 2 
Query:  116 GDMLLDKSDIALINGFKNHYANTKKWPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAI 688
            G+M L +    LIN  ++  ++   WP +      V Y F+ N+  D++ + +  + E +     CV+F  ++ Q  ++ + S   GC S++G   G   Q + L + GCM S+G ++HE  HALGF H HMR DRD+++TI    I  S  + F+K         Y+  SVMHY+   F+ +P +  +V K   +I
Sbjct:   49 GNMALTEGGDILINIGRSATSSDYLWPKSADGTVVVPYIFSYNYSADELTLFKTAMQEFETL--TCVRFVPKTIQRDFLNIVSNG-GCLSMVGRNGG--GQKVELASYGCM-SRGVIQHELNHALGFYHEHMRSDRDDYVTIITENIIPSYENYFSKRKTNNMGIIYDYNSVMHYSRNTFSISPDKSTIVPKPDPSI 239
[Close]
BLAST of Ast1 protein vs. Ensembl Xenopus
Match: ENSXETT00000032332.1 (astacin-like metallo-endopeptidase 3 gene b1 [Source:NCBI gene;Acc:100498420])

HSP 1 Score: 85.8853 bits (211), Expect = 1.876e-18
Identity = 53/168 (31.55%), Postives = 87/168 (51.79%), Query Frame = 2 
Query:  191 WPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKN 676
            WP +      V Y+F+ N+  D++ + +  + E +     CV+F   +N+  ++ + S   GC S LG   G   Q + L++ GC+Y +G ++HE  HALGF H   R DRD+++TI+   I       F K D       Y+  SVMHY+ + F+ N +  +V K +
Sbjct:   51 WPKSTDGTVIVPYNFSSNYSADQLALFKSTMQEYES--LTCVRFVPRANETDFLSIVS-DNGCASFLGKVGG--DQTVQLDSYGCIY-RGIIQHELNHALGFYHEQSRSDRDDYVTIHTENIIPGYEGNFNKADSNNLGLEYDYSSVMHYSGDAFSKNGNLTIVPKPD 212
[Close]
BLAST of Ast1 protein vs. Ensembl Xenopus
Match: ENSXETT00000032173.1 (astacin-like metallo-endopeptidase 3 gene b1 [Source:NCBI gene;Acc:100498420])

HSP 1 Score: 85.8853 bits (211), Expect = 1.978e-18
Identity = 53/168 (31.55%), Postives = 87/168 (51.79%), Query Frame = 2 
Query:  191 WPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKN 676
            WP +      V Y+F+ N+  D++ + +  + E +     CV+F   +N+  ++ + S   GC S LG   G   Q + L++ GC+Y +G ++HE  HALGF H   R DRD+++TI+   I       F K D       Y+  SVMHY+ + F+ N +  +V K +
Sbjct:  124 WPKSTDGTVIVPYNFSSNYSADQLALFKSTMQEYES--LTCVRFVPRANETDFLSIVS-DNGCASFLGKVGG--DQTVQLDSYGCIY-RGIIQHELNHALGFYHEQSRSDRDDYVTIHTENIIPGYEGNFNKADSNNLGLEYDYSSVMHYSGDAFSKNGNLTIVPKPD 285
[Close]
BLAST of Ast1 protein vs. Ensembl Xenopus
Match: ENSXETT00000032191.1 (astacin-like metallo-endopeptidase 3 gene b1 [Source:NCBI gene;Acc:100498420])

HSP 1 Score: 85.8853 bits (211), Expect = 2.093e-18
Identity = 53/168 (31.55%), Postives = 87/168 (51.79%), Query Frame = 2 
Query:  191 WPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKN 676
            WP +      V Y+F+ N+  D++ + +  + E +     CV+F   +N+  ++ + S   GC S LG   G   Q + L++ GC+Y +G ++HE  HALGF H   R DRD+++TI+   I       F K D       Y+  SVMHY+ + F+ N +  +V K +
Sbjct:  124 WPKSTDGTVIVPYNFSSNYSADQLALFKSTMQEYES--LTCVRFVPRANETDFLSIVS-DNGCASFLGKVGG--DQTVQLDSYGCIY-RGIIQHELNHALGFYHEQSRSDRDDYVTIHTENIIPGYEGNFNKADSNNLGLEYDYSSVMHYSGDAFSKNGNLTIVPKPD 285
[Close]
BLAST of Ast1 protein vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 72.7886 bits (177), Expect = 3.263e-14
Identity = 51/187 (27.27%), Postives = 79/187 (42.25%), Query Frame = 2 
Query:  125 LLDKSDIALINGFKNHYANTKKWPNN-----NVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMY-SKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVV 664
             L + DI   + F+       KWP        + +  +  +      V+     E +R    C++F     Q  ++ +     GC+S +G + GM  Q ++L  P C+   +G V HE +H LGF H H R DRD +I +NW  I       F K        PY+  SVMHY    F+      ++
Sbjct:   72 FLVEGDIIRPSPFRLLSVTNNKWPKGVGGFVEIPFLLSRKYDELSRRVIMDAFAEFERF--TCIRFVAYHGQRDFVSI-LPMAGCFSGVGRSGGM--QVVSLA-PTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFSWRGQPTII 252
[Close]
BLAST of Ast1 protein vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 72.4034 bits (176), Expect = 4.956e-14
Identity = 51/187 (27.27%), Postives = 79/187 (42.25%), Query Frame = 2 
Query:  125 LLDKSDIALINGFKNHYANTKKWPNN-----NVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMY-SKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVV 664
             L + DI   + F+       KWP        + +  +  +      V+     E +R    C++F     Q  ++ +     GC+S +G + GM  Q ++L  P C+   +G V HE +H LGF H H R DRD +I +NW  I       F K        PY+  SVMHY    F+      ++
Sbjct:   51 FLVEGDIIRPSPFRLLSVTNNKWPKGVGGFVEIPFLLSRKYDELSRRVIMDAFAEFERF--TCIRFVAYHGQRDFVSI-LPMAGCFSGVGRSGGM--QVVSLA-PTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFSWRGQPTII 231
[Close]
BLAST of Ast1 protein vs. Ensembl Mouse
Match: Astl (astacin-like metalloendopeptidase (M12 family) [Source:MGI Symbol;Acc:MGI:3046414])

HSP 1 Score: 72.4034 bits (176), Expect = 4.956e-14
Identity = 51/187 (27.27%), Postives = 79/187 (42.25%), Query Frame = 2 
Query:  125 LLDKSDIALINGFKNHYANTKKWPNN-----NVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMY-SKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVV 664
             L + DI   + F+       KWP        + +  +  +      V+     E +R    C++F     Q  ++ +     GC+S +G + GM  Q ++L  P C+   +G V HE +H LGF H H R DRD +I +NW  I       F K        PY+  SVMHY    F+      ++
Sbjct:   51 FLVEGDIIRPSPFRLLSVTNNKWPKGVGGFVEIPFLLSRKYDELSRRVIMDAFAEFERF--TCIRFVAYHGQRDFVSI-LPMAGCFSGVGRSGGM--QVVSLA-PTCLRKGRGIVLHELMHVLGFWHEHSRADRDRYIQVNWNEILPGFEINFIKSRSTNMLVPYDYSSVMHYGRFAFSWRGQPTII 231
[Close]
BLAST of Ast1 protein vs. Ensembl Mouse
Match: Mep1b (meprin 1 beta [Source:MGI Symbol;Acc:MGI:96964])

HSP 1 Score: 71.633 bits (174), Expect = 1.169e-13
Identity = 62/189 (32.80%), Postives = 92/189 (48.68%), Query Frame = 2 
Query:  128 LDKSDIAL-INGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQR-DLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLG-YAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDG---PYETESVMHYNSEGFACNPSEKVVVKK 673
            L + DI L  NG  +   + K+WP+  + Y    +   +   V+   L   +R  L  C+ F   S + +YI V  K  GC+S +G   AG   Q L++    C     TV+HEF+HALGF H   R DRD+++ I W RI+  +   F   +    D    PY+  SVMHY+   F  N +E  +V +
Sbjct:   50 LFEGDIKLEANGKNSIIGDHKRWPHT-IPYVLEDSLEMNAKGVI---LNAFERYRLKTCIDFKPWSGEANYISV-FKGSGCWSSVGNIHAG--KQELSI-GTNCD-RIATVQHEFLHALGFWHEQSRADRDDYVIIVWDRIQPGKEHNFNIYNDSVSDSLNVPYDYTSVMHYSKTAFQ-NGTESTIVTR 228
[Close]
BLAST of Ast1 protein vs. Ensembl Mouse
Match: Tll1 (tolloid-like [Source:MGI Symbol;Acc:MGI:106923])

HSP 1 Score: 71.2478 bits (173), Expect = 1.721e-13
Identity = 49/154 (31.82%), Postives = 73/154 (47.40%), Query Frame = 2 
Query:  191 WPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDG---PYETESVMHYNSEGFA 640
            WP   + Y   GNF   +  + +Q +   ++    CV FTE S++  YI    +  GC S +G     P Q +++      +  G V HE  H +GF H H R DRDNH+TI    I+  Q   F K +  E +     Y+ +S+MHY    F+
Sbjct:  157 WPGGVIPYVIGGNFTGSQRAMFKQAMRHWEKH--TCVTFTERSDEESYIVFTYRPCGCCSYVGRRGNGP-QAISIGKNCDKF--GIVVHELGHVIGFWHEHTRPDRDNHVTIIRENIQPGQEYNFLKMEPGEVNSLGERYDFDSIMHYARNTFS 305
[Close]
BLAST of Ast1 protein vs. UniProt/SwissProt
Match: sp|P55112|NAS4_CAEEL (Zinc metalloproteinase nas-4 OS=Caenorhabditis elegans OX=6239 GN=nas-4 PE=2 SV=4)

HSP 1 Score: 93.5893 bits (231), Expect = 4.799e-21
Identity = 56/171 (32.75%), Postives = 89/171 (52.05%), Query Frame = 2 
Query:  185 KKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE---SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCD---GCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNG 679
            ++WPNN + Y+ +  +      V+   + E       CVKF     S    Y+ ++   EGCYSL+G   G   QP++L++ GC+   GT+ HE +HA+GF H   R+DRD++I + W  +      QF K +       D PY+  S+MHY    F+ +  + +V KK+G
Sbjct:  102 RRWPNNEIPYTLSSQYGSYARSVIANAMNEYHTK--TCVKFVARDPSKHHDYLWIHP-DEGCYSLVGKTGG--KQPVSLDS-GCI-QVGTIVHELMHAVGFFHEQSRQDRDSYIDVVWQNVMNGADDQFEKYNLNVISHLDEPYDYASIMHYGPYAFSGSGKKTLVPKKSG 265
[Close]
BLAST of Ast1 protein vs. UniProt/SwissProt
Match: sp|P31581|HCE2_ORYLA (High choriolytic enzyme 2 OS=Oryzias latipes OX=8090 GN=hceb PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 1.757e-20
Identity = 56/155 (36.13%), Postives = 80/155 (51.61%), Query Frame = 2 
Query:  185 KKWPNNNVV--YSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFA 640
            KK  N  VV  Y  +  + R ++  +   +     +   C++F   +N+  +I V SK  GCYS LG   G   Q L+L   GCMYS G ++HE  HALGF H   R DRD+++ INW  I  +    F K D    + PY+  S+MHY  + F+
Sbjct:   92 KKASNGFVVIPYVISSQYSRGEVATIEGAMRAF--NGRTCIRFVRRTNEYDFISVVSKN-GCYSELGRKGGQ--QELSLNRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWQNIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFS 240
[Close]
BLAST of Ast1 protein vs. UniProt/SwissProt
Match: sp|P31580|HCE1_ORYLA (High choriolytic enzyme 1 OS=Oryzias latipes OX=8090 GN=hcea PE=1 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 4.706e-20
Identity = 48/116 (41.38%), Postives = 66/116 (56.90%), Query Frame = 2 
Query:  296 CVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFA 640
            C++F   +N+  +I V SK  GCYS LG   G   Q L++   GCMYS G ++HE  HALGF H   R DRD+++ INW  I  +    F K D    + PY+  S+MHY  + F+
Sbjct:  120 CIRFVRRTNEYDFISVVSKT-GCYSELGRKGGQ--QELSINRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWENIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFS 231
[Close]
BLAST of Ast1 protein vs. UniProt/SwissProt
Match: sp|P07584|ASTA_ASTAS (Astacin OS=Astacus astacus OX=6715 PE=1 SV=2)

HSP 1 Score: 89.3521 bits (220), Expect = 7.011e-20
Identity = 53/167 (31.74%), Postives = 87/167 (52.10%), Query Frame = 2 
Query:  191 WPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGP-YETESVMHYNSEGFACNPS--EKVVVKKNG 679
            W    + Y+FAG    D+  ++    G  + +   C++F   + +  Y+E+ +   GC+S +G  +G   Q ++L+  GC+Y  GT+ HE +HA+GF H H R DRDN++TIN+  ++ S  S F         G  Y+  S+MHY    F+      E +V  +NG
Sbjct:   59 WSGGVIPYTFAGVSGADQSAILS---GMQELEEKTCIRFVPRTTESDYVEIFTSGSGCWSYVGRISG--AQQVSLQANGCVY-HGTIIHELMHAIGFYHEHTRMDRDNYVTINYQNVDPSMTSNFDIDTYSRYVGEDYQYYSIMHYGKYSFSIQWGVLETIVPLQNG 219
[Close]
BLAST of Ast1 protein vs. UniProt/SwissProt
Match: sp|Q18439|NAS8_CAEEL (Zinc metalloproteinase nas-8 OS=Caenorhabditis elegans OX=6239 GN=nas-8 PE=2 SV=3)

HSP 1 Score: 91.2781 bits (225), Expect = 1.004e-19
Identity = 63/184 (34.24%), Postives = 92/184 (50.00%), Query Frame = 2 
Query:  182 TKKWPNNNVVYSFAGNF-PRDKIPVVRQCLGELQRDLGNCVKFTESN--QGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPD---GPYETESVMHYNSEGFACNPSEKVVVKKN------GGAIGYN 697
            T+KWPN  + Y  +  +  R++  + R       +    CV+F         Y+ +  K +GCYS +G A G   Q L+L+N GC+    T  HE +H++GF H H R DRD HITI W  I+     QF K D  E       Y+  S+MHY+S  F+ N  E +V K++      G AI ++
Sbjct:  118 TRKWPNGRIPYVISNQYNDRERAVLARSFQAYHDK---TCVRFVPRTAVDNDYLYIG-KIDGCYSDVGRAGGR--QELSLDN-GCL-QYDTAIHELMHSVGFYHEHERWDRDEHITILWHNIDREAYDQFGKVDLAESSYYGQLYDYYSIMHYDSLAFSKNGFETMVAKQSEMTAVIGAAIDFS 293
[Close]
BLAST of Ast1 protein vs. TrEMBL
Match: A0A060Q785 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast1 PE=2 SV=1)

HSP 1 Score: 494.197 bits (1271), Expect = 4.081e-176
Identity = 232/242 (95.87%), Postives = 237/242 (97.93%), Query Frame = 2 
Query:    2 MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQM 727
            MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCE DGPYET SVMHYNSEGFAC+P EKVV++K+GGAIGYNH+TT NDL M
Sbjct:    1 MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNM 242
[Close]
BLAST of Ast1 protein vs. TrEMBL
Match: A0A060Q714 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast2 PE=2 SV=1)

HSP 1 Score: 418.698 bits (1075), Expect = 3.804e-146
Identity = 196/244 (80.33%), Postives = 213/244 (87.30%), Query Frame = 2 
Query:    2 MQKMFLMCLAIAVIGYVSGQCGCPRQ--KLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQM 727
            M+K+ L+ L   VI Y +GQCGCPRQ  KL  R +D++KMGDMLLD +DI +INGFKNHYAN+KKWPNN V Y+FAGNFP+DKI  VRQ LGELQ+DL NCVKF E   GHYIEVNSK+EGCYS+LG   G P+QPLNLENPGCMYSKGT+KHEFIHALGFMHTHMRKDRDNHITI W RI TSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQM
Sbjct:    1 MEKLILLLLGFVVIDYANGQCGCPRQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGG-PSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQM 243
[Close]
BLAST of Ast1 protein vs. TrEMBL
Match: A0A060Q786 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast6 PE=2 SV=1)

HSP 1 Score: 380.178 bits (975), Expect = 5.513e-131
Identity = 176/242 (72.73%), Postives = 200/242 (82.64%), Query Frame = 2 
Query:    2 MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQM 727
            M+ +F + L +A + YVSGQCGCPRQKLLKR +DSM+MGDM+LDKSDIALINGFK HY+NTK+WPNN V++SFA  FP DK  +VR+CL ELQ+DLGNCVKF+ES   HYIEV+S  +GCYSLLGY  G P QPLNL+NPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITI W RI TS CSQF KC+GC+ DGPYET SVMHY S GFAC P E VV K++GG I YNH T+ NDL M
Sbjct:    1 MRSLFCLLLIVAAVSYVSGQCGCPRQKLLKRGLDSMQMGDMVLDKSDIALINGFKAHYSNTKRWPNNQVLFSFAPAFPGDKKGIVRECLVELQKDLGNCVKFSESTASHYIEVHSLNQGCYSLLGYTGG-PNQPLNLQNPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSHCSQFVKCEGCDLDGPYETNSVMHYPSYGFACVPGENVVFKRDGGLIDYNHVTSRNDLDM 241
[Close]
BLAST of Ast1 protein vs. TrEMBL
Match: I1ZIC3 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast3 PE=2 SV=1)

HSP 1 Score: 287.73 bits (735), Expect = 2.175e-94
Identity = 133/242 (54.96%), Postives = 175/242 (72.31%), Query Frame = 2 
Query:    2 MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQM 727
            MQK FL+ + + +   V+ QCGC ++KL+ R+ +++ +GDMLL K+DIA+INGFK+H    + WP   V ++FA +FP DK  +V+QCL ELQ DLGNCV+F ES QG +I V+++ +GCY+++G   G   Q LNL+NP CM SKGT+KHEFIHALGFMHTHMR DRDN I+INW  I++SQC  F KC+GC  DG YET SVMHY+S+ F C      +++KNG  I YNH TT NDL M
Sbjct:    5 MQK-FLILIVLVLANEVASQCGCNKKKLMTRATENLHVGDMLLSKADIAIINGFKHHQREARAWPGGKVSFAFANDFPADKRNLVKQCLNELQSDLGNCVQFFESTQGPHIAVHAQNKGCYAMIG-CTGERGQFLNLQNPWCMNSKGTIKHEFIHALGFMHTHMRMDRDNFISINWRNIQSSQCFNFVKCEGCIMDGNYETRSVMHYSSDAFGCQQGMNTMIQKNGNLIDYNHNTTPNDLSM 244
[Close]
BLAST of Ast1 protein vs. TrEMBL
Match: A0A060Q750 (Metalloendopeptidase OS=Schmidtea mediterranea OX=79327 GN=Ast5 PE=2 SV=1)

HSP 1 Score: 225.328 bits (573), Expect = 7.074e-70
Identity = 112/246 (45.53%), Postives = 151/246 (61.38%), Query Frame = 2 
Query:    2 MQKMFLMCLAIAVIGYV---SGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMP-TQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQM 727
            M ++F+    ++++  V   +G+CGC + KL KR++ S+K+ DMLLD  D+  I GF+ +  N ++W +N + Y+F   FP DK  +VR+ + EL  DL  CVK+ ES  G++I+V S Q GC+S +G       +Q LNLENPGC+YSKGT+KHEFIHA GFMHT MR DRD  + I W RI++  C  F  C  CE    Y+T SVMHY SE F C      +V K    I YNH T   D QM
Sbjct:    1 MNQIFIGLFLLSILNLVFIEAGKCGCQKSKL-KRAI-SLKIDDMLLDHVDLMKIEGFRGYDKNARRWTDNTLKYTFHPQFPADKKQIVRESMNELTNDLKGCVKYVESTSGYHIKVQSHQPGCWSFVGMLNKQGGSQELNLENPGCLYSKGTIKHEFIHATGFMHTQMRADRDKFVNIFWERIDSDFCHAFVTCKNCEETVTYKTNSVMHYPSESFPCPGQRITMVDKQNKEIPYNHGTIEEDHQM 244
[Close]
BLAST of Ast1 protein vs. Ensembl Cavefish
Match: c6ast1 (low choriolytic enzyme-like [Source:NCBI gene;Acc:111191947])

HSP 1 Score: 103.219 bits (256), Expect = 7.889e-26
Identity = 69/218 (31.65%), Postives = 111/218 (50.92%), Query Frame = 2 
Query:  104 SMKMGDMLLDKSDIALINGFKNHYANTKK-----WPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGH-YIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVV-VKKNGGAIGYNHETTANDL 721
            +++ GD+L+D       +G +N    T +     WP N+     + Y  +  F  ++  ++   L   ++    C++F   ++   YI+V S  EGCYS +G   G   Q L+L++ GC+Y K  ++HEF+HALGF H   R DRD HI I +  I+  +   F K D    + PY+  SVMHY  + F+ N    +V +     A+G   E +AND+
Sbjct:   54 TIRFGDILVD-------DGLQNADRCTSRKPSCMWPMNSDGNTYIPYIISNQFSPNEKDLITSVLKTFEK--STCIRFKPRDREEDYIDVQS-LEGCYSFVGRTGGR--QTLSLKSRGCLYKK-IIQHEFLHALGFHHEQCRSDRDKHIRILYENIDPGKEHNFDKKDTNNLNTPYDYGSVMHYGRDAFSRNGQPTMVPIPNQNVAVGRAEEMSANDI 258
[Close]
BLAST of Ast1 protein vs. Ensembl Cavefish
Match: c6ast1 (low choriolytic enzyme-like [Source:NCBI gene;Acc:111191947])

HSP 1 Score: 103.219 bits (256), Expect = 9.167e-26
Identity = 69/218 (31.65%), Postives = 111/218 (50.92%), Query Frame = 2 
Query:  104 SMKMGDMLLDKSDIALINGFKNHYANTKK-----WPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGH-YIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVV-VKKNGGAIGYNHETTANDL 721
            +++ GD+L+D       +G +N    T +     WP N+     + Y  +  F  ++  ++   L   ++    C++F   ++   YI+V S  EGCYS +G   G   Q L+L++ GC+Y K  ++HEF+HALGF H   R DRD HI I +  I+  +   F K D    + PY+  SVMHY  + F+ N    +V +     A+G   E +AND+
Sbjct:   65 TIRFGDILVD-------DGLQNADRCTSRKPSCMWPMNSDGNTYIPYIISNQFSPNEKDLITSVLKTFEK--STCIRFKPRDREEDYIDVQS-LEGCYSFVGRTGGR--QTLSLKSRGCLYKK-IIQHEFLHALGFHHEQCRSDRDKHIRILYENIDPGKEHNFDKKDTNNLNTPYDYGSVMHYGRDAFSRNGQPTMVPIPNQNVAVGRAEEMSANDI 269
[Close]
BLAST of Ast1 protein vs. Ensembl Cavefish
Match: ENSAMXT00000021359.2 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000034.1:1219778:1226321:-1 gene:ENSAMXG00000020723.2 transcript:ENSAMXT00000021359.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 96.2857 bits (238), Expect = 2.816e-23
Identity = 62/185 (33.51%), Postives = 93/185 (50.27%), Query Frame = 2 
Query:  188 KWPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGG-AIGYNHETTANDL 721
            KWP +      V Y  A +F   ++ V+++ L        +C++FT+ SNQ  YI + ++  GCYS +G       Q ++L+  GC+Y   TV+HE +HALGF H   R DRDNHI + W  I  S    F K +      PY+  SVM Y+   F+ N    +V   N   + G   + + ND+
Sbjct:   76 KWPKSTNGLVYVPYVIANHFTSRELQVIQRGLDSFSS--VSCIRFTQRSNQRDYISIEAR-SGCYSYVGRRGN--GQTVSLDRNGCIY-HNTVQHELLHALGFNHEQCRSDRDNHIRVVWENIVDSYKYAFDKINTLNQGTPYDYNSVMQYHRTAFSKNGQATMVPIPNANVSFGQGTQMSQNDI 254
[Close]
BLAST of Ast1 protein vs. Ensembl Cavefish
Match: npsn (low choriolytic enzyme-like [Source:NCBI gene;Acc:111191946])

HSP 1 Score: 95.9005 bits (237), Expect = 4.332e-23
Identity = 67/218 (30.73%), Postives = 106/218 (48.62%), Query Frame = 2 
Query:  104 SMKMGDMLLDKSDIALINGFKNHYANTKK-----WPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGH-YIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVV-VKKNGGAIGYNHETTANDL 721
            +++ GD+L+D       +G +N    T +     WP N+     + Y  +  F   +  ++   L   ++    C++F   ++   YI+V S  EGCYS +G   G   Q L+L   GC++ K  ++HE +HALGF H   R DRD HI I +  I   +   F K D    + PY+  SVMHY  + F+ N    +V +     A+G   E +AND+
Sbjct:   65 TIRFGDILVD-------DGLQNADRCTSRKPSCMWPRNSDGNTYIPYIISNQFRPKQKDLITSVLKTFEK--STCIRFKPRDREEDYIDVQS-LEGCYSFVGRTGG--GQTLSLNRRGCLHKK-IIQHELLHALGFHHEQCRSDRDQHIRILYENIIPGEEHNFDKEDTNNLNTPYDYGSVMHYERDAFSRNGQPTMVPIPDQNVAVGRAEEMSANDI 269
[Close]
BLAST of Ast1 protein vs. Ensembl Cavefish
Match: c6ast1 (low choriolytic enzyme-like [Source:NCBI gene;Acc:103036149])

HSP 1 Score: 95.5153 bits (236), Expect = 6.291e-23
Identity = 69/220 (31.36%), Postives = 105/220 (47.73%), Query Frame = 2 
Query:  104 SMKMGDMLLDKSDIALINGFKNHYANTKK-----WPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGN--CVKFTESNQGH-YIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVV-VKKNGGAIGYNHETTANDL 721
            ++  GD+L+D       +G +N    T +     WP N      V Y  +  F     P  ++ + ++ +   N  C++F   ++   YI + + + GCYS +G   G   Q L+L   GC+Y K  V+HEFIHALGF H   R DRD HI I +  I+  +   F K D      PY+  SVMHY  + F+ N    ++ +     A+G   E  AND+
Sbjct:   65 TITFGDILVD-------DGLQNADPCTSRNPSCLWPKNKNGIVYVPYIISNQF----CPKEKKLITDILKTFRNSTCIQFVPRDREEDYIHIQNLK-GCYSSVGRRGG--EQTLSLSRQGCLY-KRAVQHEFIHALGFHHEQSRSDRDQHIRIVYENIDPDKKHNFNKVDTNNLKTPYDYGSVMHYGRDAFSNNGQPTMIPIPDQNVAVGRAQEMNANDI 269
[Close]
BLAST of Ast1 protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007253.1 (pep scaffold:Pmarinus_7.0:GL476910:54360:144194:-1 gene:ENSPMAG00000006538.1 transcript:ENSPMAT00000007253.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 74.3294 bits (181), Expect = 2.759e-15
Identity = 50/154 (32.47%), Postives = 74/154 (48.05%), Query Frame = 2 
Query:  191 WPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDG---PYETESVMHYNSEGFA 640
            WP   + Y   GNF   +  + RQ +   ++    CV F E +++  YI    +  GC S +G   G P Q +++      +  G V HE  H +GF H H R DRD+H+TI  A I+  Q   F K +  E D     Y+ +S+MHY    F+
Sbjct:   22 WPGGVIPYIVGGNFTGSQKAMFRQAMRHWEKH--TCVTFIERTDEESYIVFTYRPCGCCSYVGRRGGGP-QAISIGKNCDKF--GIVVHELGHVIGFWHEHTRPDRDDHVTIIKANIQPGQEYNFLKMEPGEVDSLGESYDFDSIMHYARNTFS 170
[Close]
BLAST of Ast1 protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004913.1 (pep scaffold:Pmarinus_7.0:GL478359:22377:26907:1 gene:ENSPMAG00000004462.1 transcript:ENSPMAT00000004913.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 56.6102 bits (135), Expect = 3.429e-10
Identity = 28/74 (37.84%), Postives = 41/74 (55.41%), Query Frame = 2 
Query:  440 GTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDG-PYETESVMHYNSEGFACNPSEK 658
            GTV HE  HA+G++H   R DRD  I++    ++  Q  QF      +P G PY+ +SVMHY+    AC   ++
Sbjct:   25 GTVAHELGHAVGYVHEQSRPDRDAFISVLEGNVQDGQLPQFAVYSATDPRGVPYDLKSVMHYSRNVRACGTRDR 98
[Close]
BLAST of Ast1 protein vs. Ensembl Nematostella
Match: EDO34721 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNJ4])

HSP 1 Score: 90.5077 bits (223), Expect = 1.392e-21
Identity = 67/211 (31.75%), Postives = 103/211 (48.82%), Query Frame = 2 
Query:  104 SMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPD---GPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQ 724
            + ++ D L+  S  AL++   +       WPN  + Y F G          R  + E + +   C++F E +++  YI V S + GC+S +G + G   Q L+L    C   KGT  HE +H LGF H   R+DRDN+ITI+W  I++ +   F K +  + D    PY+  S+MHY    F+ N    +V K    +IG     +  DLQ
Sbjct:   12 TTRIMDQLMSPSREALVDPVSH-------WPNGRLPYKF-GAVSNSVKEAFRAAIDEYEAN--TCIRFVERTDETDYILVVS-EGGCWSPIGRSGGQ--QKLSL-GTYCD-RKGTAMHELMHTLGFFHEQSRRDRDNYITIHWDNIQSDKQFNFQKYEHGKVDTLGAPYDYGSIMHYPMFAFSSNGRATIVPKDPTASIGQRSGFSKVDLQ 207
[Close]
BLAST of Ast1 protein vs. Ensembl Nematostella
Match: EDO30445 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T0S0])

HSP 1 Score: 88.9669 bits (219), Expect = 4.200e-21
Identity = 66/208 (31.73%), Postives = 104/208 (50.00%), Query Frame = 2 
Query:  116 GDMLLDKSDIALINGFKNHYAN-TKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDG---PYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQ 724
            GD+ LD+S  A++   ++  ++  + W N  V Y F G+   +   V    + E +     CV+F     +  YI + S + GC+S +G + G+  Q L+L   GC + +GT  HE +HA+GF H   R DRD++ITI W  I+      F K    E D     Y+ +S+MHY    F  N  +  ++ +NG  IG     +A D+Q
Sbjct:    5 GDIYLDRSTKAILGMGRDAMSSPARHWLNARVPYVF-GSVSSEVRSVFLAAIKEYEAK--TCVRFVPRRGERDYIYIVS-EGGCFSSIGRSGGL--QKLSL-GTGCEH-RGTAIHEMMHAIGFFHEQSRLDRDSYITIYWDNIKPGMEYNFRKYKRGEADTLGYAYDLKSIMHYPKYAFTKN-RQPTIIARNGANIGQRDSFSAIDIQ 203
[Close]
BLAST of Ast1 protein vs. Ensembl Nematostella
Match: EDO42421 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1T1])

HSP 1 Score: 86.2705 bits (212), Expect = 1.341e-20
Identity = 59/185 (31.89%), Postives = 86/185 (46.49%), Query Frame = 2 
Query:  185 KKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTE--SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDG---PYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQ 724
            K WP   VVY     F R    + +        +   C++F +  +N   Y+ +  +  GC S +G   G   Q L+L + GC + KGT+ HE  HALGF H   R DRD+++TI +  I     S F K    E D    PY+  S+MHY  + F+ N    +V KK G   G     +  D++
Sbjct:   10 KLWPGGVVVYELDPRFERSSRAMEKLTQAISDYEKYTCIRFRKKTANDNDYVYIKDRG-GCRSHVGRKGG--KQYLSLSS-GC-WHKGTIIHELGHALGFFHEQTRPDRDDYVTILFENIRLGMASNFMKHSAGEVDSLGTPYDYGSIMHYGPKIFSANGKPTIVAKKPGVKFGQRDGLSPIDIK 189
[Close]
BLAST of Ast1 protein vs. Ensembl Nematostella
Match: EDO37770 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SEU5])

HSP 1 Score: 87.8113 bits (216), Expect = 2.627e-20
Identity = 76/246 (30.89%), Postives = 113/246 (45.93%), Query Frame = 2 
Query:    2 MQKMFLMCL-AIAVIGYVS--GQCGCPRQKLLKRSVDSMKMGDMLL--------DKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIP-VVRQCLGELQRDLGNCVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTK--CDGCEPDG-PYETESVMHYNSEGFACNPSEKVVVKKNGGAIG 691
            M+   L+C  AI+++   S     G PR  L +        GDM+L        +  D+    G   H    KKWPN  + Y    +        V++  + E +     CV+F + + Q +Y+    K +GC+S +G       Q ++L   GC + KGT  HE  HALGF H   R DRDN++T++W  I+T     F K   D     G PY+  S+MHY +  F+ N    +  KK+G  IG
Sbjct:    1 MRLSVLLCFTAISLVSSASIDENAGNPRSDLFE--------GDMILTPDQQQAVNNGDLGASRGAPIH---DKKWPNAILPYEIRSDLKGTPAEGVIQNAMREWESK--TCVRFQKRTTQKNYVYF-FKGDGCWSYVG-MLDRGAQAISL-GSGC-WHKGTAAHEIGHALGFYHEQSRPDRDNYVTVHWDNIKTDSKHNFNKYSADRINTFGTPYDFGSLMHYGTNYFSVNGRATITPKKSGAKIG 229
[Close]
BLAST of Ast1 protein vs. Ensembl Nematostella
Match: EDO41420 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4I5])

HSP 1 Score: 85.1149 bits (209), Expect = 5.404e-20
Identity = 54/184 (29.35%), Postives = 84/184 (45.65%), Query Frame = 2 
Query:  182 TKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDG---PYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQ 724
            T +WP   + Y+   +     +  +R  + ++  D   C++F E    +      +  GC S++G       Q ++L   GC Y  GTV HE  HALGF H   R DRDN++TI+W  I  +    F        D    PY+  SVMHY    F+ N    +  K++G  +G   + +  D+Q
Sbjct:   38 TGRWPEGVMAYTIDRSLSNRAMNAIRAGM-KMWTD-NTCIRFKERTNEYAYARFWRGRGCSSMVGRTG--RRQDISLAG-GCWY-PGTVAHEIGHALGFYHEQSRPDRDNYVTIHWNNISPNMKFNFNMYPRSTIDSLGTPYDYRSVMHYQGNAFSSNGRATITAKQSGVTLGNRRQLSKVDIQ 215
[Close]
BLAST of Ast1 protein vs. Ensembl Medaka
Match: mc6ast4 (astacin like metallo-protease [Source:NCBI gene;Acc:100049317])

HSP 1 Score: 101.679 bits (252), Expect = 2.894e-25
Identity = 59/195 (30.26%), Postives = 102/195 (52.31%), Query Frame = 2 
Query:  116 GDMLLDKSDIALINGFKNH--YANTKKWPNN-----NVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKN 676
            G   L + DIA+  G ++   +A +  W  +      + Y+ + ++    + ++++ +  ++   G CV+F  +++Q  Y+++  K  GC+S LG   G   Q L+L+NP CM   G + HEF+HALGF+H   R DRDN+IT+ W  I   +   F K      D PY+  S+MH+    ++ +    +V K N
Sbjct:   62 GTTTLREGDIAVSTGRRSKMCFARSCLWSKSVDGHVYIAYTISPDYTEADVKLIKKGMENIEE--GTCVRFVPQTHQRDYVDIQPK-SGCWSYLGARGG--RQTLSLQNPDCM-QVGVISHEFMHALGFVHEQSRFDRDNYITVMWPNIWRDRLRNFEKFKTDSLDLPYDYGSIMHFGMYAYSMDGQPTLVAKNN 250
[Close]
BLAST of Ast1 protein vs. Ensembl Medaka
Match: mc6ast5 (astacin like metallo-protease [Source:NCBI gene;Acc:100049509])

HSP 1 Score: 97.4413 bits (241), Expect = 1.345e-23
Identity = 47/130 (36.15%), Postives = 74/130 (56.92%), Query Frame = 2 
Query:  290 GNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKN 676
            G C+KF   +++ H++++  +  GC+S LG   G  +Q L+L+ PGCM+S G   HEF+HALGF+H   R DRD+++TI W  I       F K      + PY+  SVMHY    F+ +    ++ + +
Sbjct:  129 GTCIKFVPRTHEAHFLDIQPRY-GCWSFLGQTGG--SQTLSLQTPGCMWS-GIAAHEFMHALGFVHEQSRSDRDHYVTIVWKNIMPDHAHNFRKQATNNLNSPYDYSSVMHYGRYAFSGDGGPTIIPRPD 254
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BLAST of Ast1 protein vs. Ensembl Medaka
Match: ENSORLT00000018637.2 (pep primary_assembly:ASM223467v1:3:32702055:32705063:-1 gene:ENSORLG00000014863.2 transcript:ENSORLT00000018637.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 91.6633 bits (226), Expect = 1.672e-21
Identity = 49/116 (42.24%), Postives = 66/116 (56.90%), Query Frame = 2 
Query:  296 CVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFA 640
            C++F   +N+  +I V SK  GCYS LG   G   Q L+L   GCMYS G ++HE  HALGF H   R DRD+++ INW  I  +    F K D    + PY+  S+MHY  + F+
Sbjct:  120 CIRFVRRTNEYDFISVVSK-NGCYSELGRKGGQ--QELSLNRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWQNIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFS 231
[Close]
BLAST of Ast1 protein vs. Ensembl Medaka
Match: mnep (nephrosin [Source:NCBI gene;Acc:100049315])

HSP 1 Score: 91.6633 bits (226), Expect = 1.878e-21
Identity = 62/213 (29.11%), Postives = 104/213 (48.83%), Query Frame = 2 
Query:  113 MGDMLLDKSDIALINGFKNHYANTKK---WPNNN-----VVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFT-ESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVV-VKKNGGAIGYNHETTANDL 721
            + D L+   DIA+  G +N    T +   WP ++     V +  +G F   +   + Q L   +     C++FT   +Q  ++++ S+  GC+S +G   G   Q ++L   GC++  GT++HE +HALGF H   R DRD+H+ I    + + Q   F K +      PY+  S+MHY    F+ N    +V +      IG   E ++ND+
Sbjct:   58 LDDPLVMFGDIAVPTGLQNADPCTARGCLWPKSSDGNVYVPFRISGQFSSRERDTILQGLRSFEG--STCIRFTPHQSQRDFVDIQSRT-GCWSFVGRRGG--GQVVSLMRQGCVF-MGTIQHELLHALGFNHEQTRSDRDSHVRILLQNVISGQEHNFRKIETRNLGTPYDYNSIMHYGRFAFSRNREPTIVPIPDPNVPIGRATEMSSNDI 264
[Close]
BLAST of Ast1 protein vs. Ensembl Medaka
Match: hce (Oryzias latipes protease (hce), mRNA. [Source:RefSeq mRNA;Acc:NM_001201498])

HSP 1 Score: 91.2781 bits (225), Expect = 2.063e-21
Identity = 48/116 (41.38%), Postives = 67/116 (57.76%), Query Frame = 2 
Query:  296 CVKFTE-SNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFA 640
            C++F   +N+  +I V SK  GCYS LG   G+  Q L++   GCMYS G ++HE  HALGF H   R DRD+++ INW  I  +    F K D    + PY+  S+MHY  + F+
Sbjct:  129 CIRFVRRTNEYDFISVVSKT-GCYSELGRKGGL--QELSINRGGCMYS-GIIQHELNHALGFQHEQTRSDRDSYVRINWENIIPASAYNFNKHDTNNLNTPYDYSSIMHYGRDAFS 240
[Close]
BLAST of Ast1 protein vs. Planmine SMEST
Match: SMESG000015174.1 (SMESG000015174.1)

HSP 1 Score: 494.197 bits (1271), Expect = 1.135e-179
Identity = 232/242 (95.87%), Postives = 237/242 (97.93%), Query Frame = 2 
Query:    2 MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQM 727
            MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCE DGPYET SVMHYNSEGFAC+P EKVV++K+GGAIGYNH+TT NDL M
Sbjct:    1 MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNM 242
[Close]
BLAST of Ast1 protein vs. Planmine SMEST
Match: SMESG000015174.1 (SMESG000015174.1)

HSP 1 Score: 488.419 bits (1256), Expect = 2.055e-177
Identity = 229/239 (95.82%), Postives = 234/239 (97.91%), Query Frame = 2 
Query:   11 MFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQM 727
            MFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCE DGPYET SVMHYNSEGFAC+P EKVV++K+GGAIGYNH+TT NDL M
Sbjct:    1 MFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNM 239
[Close]
BLAST of Ast1 protein vs. Planmine SMEST
Match: SMESG000015174.1 (SMESG000015174.1)

HSP 1 Score: 481.871 bits (1239), Expect = 7.762e-175
Identity = 226/236 (95.76%), Postives = 231/236 (97.88%), Query Frame = 2 
Query:   20 MCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQM 727
            MCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCE DGPYET SVMHYNSEGFAC+P EKVV++K+GGAIGYNH+TT NDL M
Sbjct:    1 MCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCELDGPYETTSVMHYNSEGFACDPREKVVLRKDGGAIGYNHDTTPNDLNM 236
[Close]
BLAST of Ast1 protein vs. Planmine SMEST
Match: SMESG000072807.1 (SMESG000072807.1)

HSP 1 Score: 391.349 bits (1004), Expect = 4.751e-139
Identity = 186/238 (78.15%), Postives = 201/238 (84.45%), Query Frame = 2 
Query:   20 MCLAIAVIGYVSGQCGCPRQ--KLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQM 727
            M  +  +I Y      C  Q  KL  R +D++KMGDMLLD +DI +INGFKNHYAN+KKWPNN V Y+FAGNFP+DKI  VRQ LGELQ+DL NCVKF E   GHYIEVNSK+EGCYS+LG   G P+QPLNLENPGCMYSKGT+KHEFIHALGFMHTHMRKDRDNHITI W RI TSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQM
Sbjct:    1 MSTSSEIIYYYQASSFCLIQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGG-PSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQM 237
[Close]
BLAST of Ast1 protein vs. Planmine SMEST
Match: SMESG000072805.1 (SMESG000072805.1)

HSP 1 Score: 391.349 bits (1004), Expect = 4.751e-139
Identity = 186/238 (78.15%), Postives = 201/238 (84.45%), Query Frame = 2 
Query:   20 MCLAIAVIGYVSGQCGCPRQ--KLLKRSVDSMKMGDMLLDKSDIALINGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCVKFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVKHEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQM 727
            M  +  +I Y      C  Q  KL  R +D++KMGDMLLD +DI +INGFKNHYAN+KKWPNN V Y+FAGNFP+DKI  VRQ LGELQ+DL NCVKF E   GHYIEVNSK+EGCYS+LG   G P+QPLNLENPGCMYSKGT+KHEFIHALGFMHTHMRKDRDNHITI W RI TSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQM
Sbjct:    1 MSTSSEIIYYYQASSFCLIQSVKLRTRGLDNVKMGDMLLDSTDIQIINGFKNHYANSKKWPNNIVTYTFAGNFPQDKIQTVRQSLGELQKDLQNCVKFQEGRNGHYIEVNSKREGCYSMLGVTGG-PSQPLNLENPGCMYSKGTIKHEFIHALGFMHTHMRKDRDNHITIKWDRILTSQCSQFTKCDGCEPDGPYETESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQM 237
The following BLAST results are available for this feature:
BLAST of Ast1 protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ASTL1.062e-1731.28astacin like metalloendopeptidase [Source:HGNC Sym... [more]
TLL14.362e-1330.77tolloid like 1 [Source:HGNC Symbol;Acc:HGNC:11843][more]
BMP15.560e-1330.13bone morphogenetic protein 1 [Source:HGNC Symbol;A... [more]
BMP16.543e-1330.13bone morphogenetic protein 1 [Source:HGNC Symbol;A... [more]
BMP17.676e-1330.13bone morphogenetic protein 1 [Source:HGNC Symbol;A... [more]
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BLAST of Ast1 protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nas-43.773e-2232.75Zinc metalloproteinase nas-4 [Source:UniProtKB/Sw... [more]
nas-41.420e-2132.56Zinc metalloproteinase nas-4 [Source:UniProtKB/Sw... [more]
nas-41.659e-2132.56Zinc metalloproteinase nas-4 [Source:UniProtKB/Sw... [more]
nas-87.896e-2134.24Zinc metalloproteinase nas-8 [Source:UniProtKB/Sw... [more]
nas-71.285e-2028.70Zinc metalloproteinase nas-7 [Source:UniProtKB/Sw... [more]
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BLAST of Ast1 protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG67632.884e-3333.33gene:FBgn0039069 transcript:FBtr0084378[more]
CG67632.884e-3333.33gene:FBgn0039069 transcript:FBtr0334497[more]
CG118655.457e-2232.79gene:FBgn0028947 transcript:FBtr0080753[more]
CG152553.292e-1931.12gene:FBgn0028950 transcript:FBtr0080785[more]
CG152553.292e-1931.12gene:FBgn0028950 transcript:FBtr0343719[more]
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BLAST of Ast1 protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hce2l27.377e-2231.58high choriolytic enzyme 2-like 2 [Source:ZFIN;Acc:... [more]
c6ast37.433e-2230.98six-cysteine containing astacin protease 3 [Source... [more]
c6ast37.433e-2230.98six-cysteine containing astacin protease 3 [Source... [more]
c6ast47.782e-2230.43six-cysteine containing astacin protease 4 [Source... [more]
c6ast47.782e-2230.43six-cysteine containing astacin protease 4 [Source... [more]
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BLAST of Ast1 protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSXETT00000022029.11.924e-2030.77astacin-like metalloendopeptidase [Source:NCBI gen... [more]
kpna47.813e-2031.47karyopherin alpha 4 (importin alpha 3) [Source:Xen... [more]
ENSXETT00000032332.11.876e-1831.55astacin-like metallo-endopeptidase 3 gene b1 [Sour... [more]
ENSXETT00000032173.11.978e-1831.55astacin-like metallo-endopeptidase 3 gene b1 [Sour... [more]
ENSXETT00000032191.12.093e-1831.55astacin-like metallo-endopeptidase 3 gene b1 [Sour... [more]
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BLAST of Ast1 protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Astl3.263e-1427.27astacin-like metalloendopeptidase (M12 family) [So... [more]
Astl4.956e-1427.27astacin-like metalloendopeptidase (M12 family) [So... [more]
Astl4.956e-1427.27astacin-like metalloendopeptidase (M12 family) [So... [more]
Mep1b1.169e-1332.80meprin 1 beta [Source:MGI Symbol;Acc:MGI:96964][more]
Tll11.721e-1331.82tolloid-like [Source:MGI Symbol;Acc:MGI:106923][more]
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BLAST of Ast1 protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P55112|NAS4_CAEEL4.799e-2132.75Zinc metalloproteinase nas-4 OS=Caenorhabditis ele... [more]
sp|P31581|HCE2_ORYLA1.757e-2036.13High choriolytic enzyme 2 OS=Oryzias latipes OX=80... [more]
sp|P31580|HCE1_ORYLA4.706e-2041.38High choriolytic enzyme 1 OS=Oryzias latipes OX=80... [more]
sp|P07584|ASTA_ASTAS7.011e-2031.74Astacin OS=Astacus astacus OX=6715 PE=1 SV=2[more]
sp|Q18439|NAS8_CAEEL1.004e-1934.24Zinc metalloproteinase nas-8 OS=Caenorhabditis ele... [more]
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BLAST of Ast1 protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A060Q7854.081e-17695.87Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7143.804e-14680.33Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7865.513e-13172.73Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
I1ZIC32.175e-9454.96Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
A0A060Q7507.074e-7045.53Metalloendopeptidase OS=Schmidtea mediterranea OX=... [more]
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BLAST of Ast1 protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
c6ast17.889e-2631.65low choriolytic enzyme-like [Source:NCBI gene;Acc:... [more]
c6ast19.167e-2631.65low choriolytic enzyme-like [Source:NCBI gene;Acc:... [more]
ENSAMXT00000021359.22.816e-2333.51pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
npsn4.332e-2330.73low choriolytic enzyme-like [Source:NCBI gene;Acc:... [more]
c6ast16.291e-2331.36low choriolytic enzyme-like [Source:NCBI gene;Acc:... [more]
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BLAST of Ast1 protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000007253.12.759e-1532.47pep scaffold:Pmarinus_7.0:GL476910:54360:144194:-1... [more]
ENSPMAT00000004913.13.429e-1037.84pep scaffold:Pmarinus_7.0:GL478359:22377:26907:1 g... [more]
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BLAST of Ast1 protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Ast1 protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO347211.392e-2131.75Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO304454.200e-2131.73Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO424211.341e-2031.89Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO377702.627e-2030.89Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO414205.404e-2029.35Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Ast1 protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mc6ast42.894e-2530.26astacin like metallo-protease [Source:NCBI gene;Ac... [more]
mc6ast51.345e-2336.15astacin like metallo-protease [Source:NCBI gene;Ac... [more]
ENSORLT00000018637.21.672e-2142.24pep primary_assembly:ASM223467v1:3:32702055:327050... [more]
mnep1.878e-2129.11nephrosin [Source:NCBI gene;Acc:100049315][more]
hce2.063e-2141.38Oryzias latipes protease (hce), mRNA. [Source:RefS... [more]
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BLAST of Ast1 protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000015174.11.135e-17995.87SMESG000015174.1[more]
SMESG000015174.12.055e-17795.82SMESG000015174.1[more]
SMESG000015174.17.762e-17595.76SMESG000015174.1[more]
SMESG000072807.14.751e-13978.15SMESG000072807.1[more]
SMESG000072805.14.751e-13978.15SMESG000072805.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30027262
GenBankCCW36839.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027262 ID=SMED30027262|Name=Ast1 protein|organism=Schmidtea mediterranea sexual|type=transcript|length=805bp
CATGCAAAAAATGTTTTTGATGTGTTTGGCGATTGCCGTTATCGGTTACG
TTTCTGGTCAATGTGGGTGCCCTAGACAGAAACTGCTGAAGAGGTCTGTA
GATAGCATGAAAATGGGAGACATGTTATTGGATAAATCTGATATTGCACT
AATAAACGGCTTTAAAAACCATTATGCTAATACTAAAAAATGGCCTAACA
ACAATGTTGTATACTCTTTTGCTGGAAACTTTCCACGAGACAAGATTCCA
GTCGTTAGACAATGCTTGGGAGAATTGCAACGAGATTTGGGAAACTGTGT
CAAGTTCACCGAATCCAATCAAGGCCATTATATTGAAGTCAACTCAAAAC
AAGAAGGCTGCTATTCACTGTTGGGATATGCCGCAGGAATGCCTACCCAA
CCATTGAATTTGGAAAATCCCGGATGCATGTATTCAAAAGGTACAGTTAA
GCACGAATTCATTCATGCACTCGGATTCATGCATACACATATGAGAAAAG
ACAGAGACAATCATATTACTATTAATTGGGCAAGAATTGAAACAAGTCAA
TGCAGTCAGTTTACGAAATGCGACGGATGCGAACCCGACGGACCGTACGA
GACTGAGAGCGTCATGCATTACAACAGCGAAGGATTCGCTTGTAATCCAA
GCGAGAAAGTCGTGGTGAAGAAAAATGGAGGAGCGATCGGATACAATCAC
GAGACGACAGCCAATGATTTGCAAATGATCAAGAATTTTTACAACTGCAA
ATAAAATTATATAAATAATAAATAAATTATTTATCTTGAAAAAAAAAGAT
CGGAA
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protein sequence of SMED30027262-orf-1

>SMED30027262-orf-1 ID=SMED30027262-orf-1|Name=SMED30027262-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=251bp
MQKMFLMCLAIAVIGYVSGQCGCPRQKLLKRSVDSMKMGDMLLDKSDIAL
INGFKNHYANTKKWPNNNVVYSFAGNFPRDKIPVVRQCLGELQRDLGNCV
KFTESNQGHYIEVNSKQEGCYSLLGYAAGMPTQPLNLENPGCMYSKGTVK
HEFIHALGFMHTHMRKDRDNHITINWARIETSQCSQFTKCDGCEPDGPYE
TESVMHYNSEGFACNPSEKVVVKKNGGAIGYNHETTANDLQMIKNFYNCK
*
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Aliases
The feature 'Ast1 protein' has the following synonyms
Synonym
Ast1
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000420parapharyngeal region
PLANA:0000429neoblast
Vocabulary: INTERPRO
TermDefinition
IPR001506Peptidase_M12A
IPR006026Peptidase_Metallo
IPR024079MetalloPept_cat_dom_sf
Vocabulary: molecular function
TermDefinition
GO:0004222metalloendopeptidase activity
GO:0008237metallopeptidase activity
GO:0008270zinc ion binding
GO:0008233peptidase activity
GO:0016787hydrolase activity
GO:0046872metal ion binding
Vocabulary: biological process
TermDefinition
GO:0006508proteolysis
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001506Peptidase M12APRINTSPR00480ASTACINcoord: 143..161
score: 45.53
coord: 162..179
score: 42.22
coord: 198..213
score: 43.33
IPR001506Peptidase M12APFAMPF01400Astacincoord: 62..221
e-value: 2.5E-36
score: 125.1
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 60..203
e-value: 1.4E-21
score: 87.7
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3DG3DSA:3.40.390.10coord: 25..230
e-value: 1.8E-42
score: 147.4
NoneNo IPR availablePANTHERPTHR10127DISCOIDIN, CUB, EGF, LAMININ , AND ZINC METALLOPROTEASE DOMAIN CONTAININGcoord: 40..217
NoneNo IPR availablePROSITEPS51864ASTACINcoord: 49..230
score: 39.705
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..19
score: 0.859
NoneNo IPR availableSUPERFAMILYSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 60..217