SMED30027246

Overview
NameSMED30027246
Smed IDSMED30027246
Length (bp)1898
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30027246 (SMED30027246) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30027246 (SMED30027246) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30027246 (SMED30027246) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30027246 (SMED30027246) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30027246

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30027246SMESG000047430.1 dd_Smed_v4_20269_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30027246SMESG000047430.1 dd_Smed_v4_20269_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30027246SMESG000047430.1 dd_Smed_v4_20269_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30027246SMESG000047430.1 dd_Smed_v4_20269_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30027246SMESG000047430.1 dd_Smed_v4_20269_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027246SMESG000047430.1 Contig22762newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
tail regionSMED30027246SMESG000047430.1 dd_Smed_v6_20269_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
muscle cellSMED30027246SMESG000047430.1 dd_Smed_v4_20269_0_1dd_Smed_v4PMID:30962434
Cote et al., 2019		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30027246 vs. Ensembl Human
Match: GPC5 (glypican 5 [Source:HGNC Symbol;Acc:HGNC:4453])

HSP 1 Score: 51.9878 bits (123), Expect = 1.637e-6
Identity = 47/214 (21.96%), Postives = 91/214 (42.52%), Query Frame = 3 
Query:  693 KTKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIE--NDWKFGI--IGAIQTFYDELMR-IILKLHIP-----DEFNKKDNR---------RCSMNS-----FLKYLTENATLYEESQRNASIHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNG 1262
             +++ +   N+ I ++++           +CS  +L+M +C +C G   T PC   CL ++  CL+ +  ++  W      +E  +D   G   IG +   +  L+   +L+ H+      ++ N+   R         RCS +       +K  T N+   EE+  N     +      +R  +S +G LA+ +C N     + ++   CWNG
Sbjct:  113 PSRTFLQALNLGIEVINT---TDYLHFSKECSRALLKMQYCPHCQGLALTKPCMGYCLNVMRGCLAHMAELNPHWHAYIRSLEELSDAMHGTYDIGHVLLNFHLLVNDAVLQAHLNGQKLLEQVNRICGRPVRTPTQSPRCSFDQSKEKHGMKTTTRNS---EETLANRRKEFI----NSLRLYRSFYGGLADQLCANE---LAAADGLPCWNG 313
[Close]
BLAST of SMED30027246 vs. Ensembl Human
Match: GPC5 (glypican 5 [Source:HGNC Symbol;Acc:HGNC:4453])

HSP 1 Score: 50.8322 bits (120), Expect = 6.867e-6
Identity = 47/213 (22.07%), Postives = 91/213 (42.72%), Query Frame = 3 
Query:  696 TKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIE--NDWKFGI--IGAIQTFYDELMR-IILKLH-----IPDEFNKKDNR---------RCSMNS-----FLKYLTENATLYEESQRNASIHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNG 1262
            +++ +   N+ I ++++           +CS  +L+M +C +C G   T PC   CL ++  CL+ +  ++  W      +E  +D   G   IG +   +  L+   +L+ H     + ++ N+   R         RCS +       +K  T N+   EE+  N     +      +R  +S +G LA+ +C N     + ++   CWNG
Sbjct:  222 SRTFLQALNLGIEVINTTDYLH---FSKECSRALLKMQYCPHCQGLALTKPCMGYCLNVMRGCLAHMAELNPHWHAYIRSLEELSDAMHGTYDIGHVLLNFHLLVNDAVLQAHLNGQKLLEQVNRICGRPVRTPTQSPRCSFDQSKEKHGMKTTTRNS---EETLANRRKEFI----NSLRLYRSFYGGLADQLCANE---LAAADGLPCWNG 421
[Close]
BLAST of SMED30027246 vs. UniProt/SwissProt
Match: sp|P50593|GPC1_CHICK (Glypican-1 OS=Gallus gallus OX=9031 GN=GPC1 PE=2 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 3.082e-6
Identity = 45/194 (23.20%), Postives = 78/194 (40.21%), Query Frame = 3 
Query:  741 DSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIEN-----DWKFGIIGAIQTFYDELMRIILKLH------IPDEFNKKDNRRCSM---NSFLKYLTENATLYEESQRNASIHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNGSASSSY 1280
            D + K     + ++C+  ++++ +C +C G     PC + CL ++  CL++   ++  W  L + +       D  + +   I T +  +   I  L           F    N + SM   +S  K      TL  +S   A   LV +A   +   KS W  L  ++C       S +  +KCWNG    SY
Sbjct:  229 DVVKKVSQVSLSHECTRAVMKLMYCPHCRGMASVKPCSNYCLNVVKGCLANQADLNTEWKYLMDALVAVADRIDGPYNVDTVIGTIHMRISEAISNLQENKVSITAKVFQGCGNPKVSMKGSSSEDKKRRGKVTLEAKSSAVALEMLVLDAKGNLTALKSYWVTLPGVLCSKK-IMASPAEDDKCWNGMTKGSY 421
[Close]
BLAST of SMED30027246 vs. TrEMBL
Match: A0A4S2LLC7 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_006356 PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.363e-10
Identity = 52/225 (23.11%), Postives = 98/225 (43.56%), Query Frame = 3 
Query:  705 LVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIE-------NDWKFGIIGAIQTFYDELM---RIILKLHIPDEFNKKDNRRCSMNSFLKYLTE--NATLYEESQRNAS--------IHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSN-TNKCWNGSASSSYTWHQCSFCENTQ 1316
            L+    +++ +++ L +      Q  C+  ++RM HCA CAGK  T PC  +CL  ++ CL  L  +   W +  + I+          + G++   +     L+   R +LK H           +CS  + L  L E      YEE   ++S          ++    T++    ++W + +  +C  +P     S   ++CWNG+A + +T   C FC+ ++
Sbjct:  348 LMQSLEVAVVLMEGLKEFNAA--QGTCAYKLMRMHHCALCAGKTLTRPCPELCLSTLSNCLKPLSQLDAPWNRFIDAIKLVAQTFLQKPQLGLMVQFKQLPKRLIGYFRHLLKTH-----KHWLQPQCSGTTKLLDLFEMGGPISYEEEAPSSSEMNILRSHQAIMKEMVTKMEQLSNIWSSASSAICTESPHLVPTSKMPDQCWNGTAQARFTPSACEFCDTSE 565
[Close]
BLAST of SMED30027246 vs. TrEMBL
Match: A0A4S2LLC9 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_006356 PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 1.397e-10
Identity = 52/225 (23.11%), Postives = 98/225 (43.56%), Query Frame = 3 
Query:  705 LVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIE-------NDWKFGIIGAIQTFYDELM---RIILKLHIPDEFNKKDNRRCSMNSFLKYLTE--NATLYEESQRNAS--------IHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSN-TNKCWNGSASSSYTWHQCSFCENTQ 1316
            L+    +++ +++ L +      Q  C+  ++RM HCA CAGK  T PC  +CL  ++ CL  L  +   W +  + I+          + G++   +     L+   R +LK H           +CS  + L  L E      YEE   ++S          ++    T++    ++W + +  +C  +P     S   ++CWNG+A + +T   C FC+ ++
Sbjct:  348 LMQSLEVAVVLMEGLKEFNAA--QGTCAYKLMRMHHCALCAGKTLTRPCPELCLSTLSNCLKPLSQLDAPWNRFIDAIKLVAQTFLQKPQLGLMVQFKQLPKRLIGYFRHLLKTH-----KHWLQPQCSGTTKLLDLFEMGGPISYEEEAPSSSEMNILRSHQAIMKEMVTKMEQLSNIWSSASSAICTESPHLVPTSKMPDQCWNGTAQARFTPSACEFCDTSE 565
[Close]
BLAST of SMED30027246 vs. TrEMBL
Match: A0A075AHD5 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_03712 PE=3 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.686e-9
Identity = 48/202 (23.76%), Postives = 90/202 (44.55%), Query Frame = 3 
Query:  774 QNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIE-------NDWKFGIIGAIQTFYDELM---RIILKLHIPDEFNKKDNRRCSMNSFLKYLTE--NATLYEESQRNAS--------IHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSN-TNKCWNGSASSSYTWHQCSFCENTQ 1316
            Q  C+  ++RM HCA CAGK  T PC  +CL  ++ CL  L  +   W +  + I+          + G++   +     L+   R +LK H      +    +CS  + L  L E      YEE   ++S          ++ +   ++    ++W + +  +C  +P     S   ++CWNG+A + ++ + C FC+ ++
Sbjct:  450 QGTCAYKLMRMHHCALCAGKTLTRPCPELCLSTLSNCLKPLSQLDAPWNRFIDAIKLVAQTFLQKPQLGLMVQFKQLPKRLIGYFRHLLKTH-----KQWLQPQCSGTTKLLELFEMGGPISYEEEAPSSSEMNILRSHQAIMKDMVLKMEQLSNIWSSASSSICNESPHLVPTSKMPDQCWNGTAQARFSANACEFCDTSE 646
[Close]
BLAST of SMED30027246 vs. TrEMBL
Match: A0A1A9W3G9 (Uncharacterized protein OS=Glossina brevipalpis OX=37001 PE=3 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 7.503e-7
Identity = 69/296 (23.31%), Postives = 124/296 (41.89%), Query Frame = 3 
Query:  450 SLEEVLTEFLAKLFQASVVNPCRENQDLYLSDYQNRKTRHRFHS--KHRLSRIVLLQWVKRNGGSDLVQSLTKAIDMFVSEINKTKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAG--KVFTIPCFSVCLQIINKCLSDLQ-IISELWIKLFEMIEN------DWKFGIIGAIQTFYDELMRIILKL--HIPDEFNKKDNRRCSMNSFLKYLT---ENATLYEESQRNASI--HLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNGSASSSYT 1283
            SLE+V+ +F  +LF      P   +Q ++LS   N +    + +  KH    ++    + +    +L+QS+  A  +F+  ++++  +       +S  D LY      +   C  ++L+MS+C  C G  KV   PC+S C+ ++  C +    ++   W  + + I+N          GI+  I+    +L   I+    H PD  NK   + C     L  LT   E   L + S + AS     +    + +   K  + N+   VC++        N + CW G     YT
Sbjct:   30 SLEDVIHKFFVQLF------PVAYHQAVHLSKNNNGELHEDYINCLKHTFDDLLPFGQIPKELSRNLMQSVQTA-RIFMQALHQSAEV-------LSETDELYASH---LSASCQKHLLKMSYCPSCTGLSKVHVKPCYSYCMNVLRGCSAQYAGVLDSPWSNVVDAIDNLVTTHIRSDAGIVTIIKQLDSKLSEAIMLAMEHGPDLENKV-KKTCGTPKLLPSLTINNEGKPLTQHSVKWASPADSEILRFLSTIDKSKEFYSNIVNNVCEDE---DIQRNDHHCWTGDRIGDYT 304
[Close]
BLAST of SMED30027246 vs. TrEMBL
Match: A0A437CFM0 (Uncharacterized protein OS=Oryzias javanicus OX=123683 GN=OJAV_G00174610 PE=3 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 8.861e-7
Identity = 89/396 (22.47%), Postives = 155/396 (39.14%), Query Frame = 3 
Query:  168 SCCTIPNENSLKSISTDDYLEIIRRLIKTNREKLNHLQNRFLEFHKAVIMIFANKFDFL---------KILPMKSQLRNGDQMAVKLFENNLFSYINNIEITQSLEEVLTEFLAKLFQASVVNPCRENQDLYLS-DY------QNRKTRHRFHSKHRLSRIVLLQWVKRNGGSDLVQSLTKAIDMFVSEINKTKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIENDWKFGIIG--------------AIQTFYDELMRIILKLHIP-DEFNKKDNRRCSMNSFLKYLTENATLYEESQRNASIHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNG 1262
            +CCT+  E +L S+S  +   +++       +    LQ   L  HKA    F    D L              +S L   +        N+L  Y     I  +LEEVL EF AKL +  V    ++N+ + L  DY      Q    R    S H++S +V   ++              A   F+  + K+  +V              K     +   C   ++RM++C +C G     PC + C  ++  CL++   ++  W  L + + N  +  +IG              AI T  +++  I  KL +   E          +++  +   E+ TL + S+    I LV +  T +R  +S W +L  ++C +   + + +  +KCWNG
Sbjct:   65 TCCTVEMEGNLASLSCKEMEGLLK-------DAGRALQTSLLGQHKAFDGFF---LDLLNCSMARLQRSFTSTRSLLHLMNSQVFSDLYNDLSGYCRGASI--NLEEVLNEFWAKLLEKLVY---QKNKQISLGEDYLECVSKQIETIRPFGESPHKMSVVVTRTFL--------------AARSFLQGLMKSAEVVR-------------KVSQVPLSQACMRAMMRMTYCPHCRGIPSARPCANYCSNVMKGCLANQADLNTEWKHLVDTMVNVAENRLIGMDQMILSLPDHISEAISTMMEKIDIINAKLSLACGEVGPISTEGEEVSTAGRVTVED-TLGDTSKN--MIKLVSDVTTRLRAMQSHWVSLPNVLCSDRAAYGAGAE-DKCWNG 414
[Close]
BLAST of SMED30027246 vs. Ensembl Cavefish
Match: gpc4 (glypican-4 [Source:NCBI gene;Acc:103025650])

HSP 1 Score: 56.6102 bits (135), Expect = 4.916e-8
Identity = 79/410 (19.27%), Postives = 149/410 (36.34%), Query Frame = 3 
Query:  165 LSCCTIPNENSLKSISTDDYLEIIRRLIKTNREKLNHLQNRFLEFHKAVIM--------IFANKFDFLKILPMKSQLRNGDQMAVKLFENNLFSYINNIEITQSLEEVLTEFLAKLFQASVVNPCRENQDLYLSDYQNRKTRHRFHSKHRLSRIVLLQWVKRNGGSDLVQSLTKAIDMFVSEINK-----TKSLV--NVFNISISIL-DSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELW-------IKLFEMIENDWKF---------GIIGAIQTFYDELMRIILKL------HIPDEFNKKDNRRCSMNSFLKYLTENATLYEESQRNASIHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNGSASSSY 1280
             SCCT+  E  L   S  D    + +L  T +         F +F K ++         +F   +  + +       +N +    K F + L  Y  +      L+ +L+EF A+L +                               R+ R+V +Q+   +G  + V   T  +  F     K     T++ V    F + ++++ D + K         C    ++M +C YC+G+V   PC + CL ++  CL++   +   W       + L E +E  + F          I  AI    +  M++  K+        P   +K+  +        +  + +      +  N    LV +   ++++ K  W  L + VC        ++  + CWNG+A S Y
Sbjct:   63 FSCCTLEMEEKLSQQSRTDLKAPVSQLSSTLQSTFTQRHQHFDQFFKELLTNAEKSLHDMFVRTYGVMYV-------KNAELF--KHFFHELRRYYASGGAAVHLDSMLSEFWAELLE-------------------------------RMFRLVNVQYEFTDGYMECVSRHTDQLKPFGDVPRKLKLQLTRAFVAARTFTLGLALIPDVVSKVSTVSASPSCVRAAMKMLYCPYCSGQVALKPCKNYCLNVMRGCLANQADLDTEWNNFLDAMLSLAERLEGPFNFESVMDPIDVKISDAIMNMQENSMQVSQKVFQGCGQPKPMYRSKRSTKDSGFPGRFRPYSPDTRPTTAAGTNMD-RLVTDVKKKLKHAKKFWSTLPDTVCVG----DRVAQADDCWNGTAKSRY 427
[Close]
BLAST of SMED30027246 vs. Ensembl Cavefish
Match: gpc1a (glypican 1a [Source:NCBI gene;Acc:553367])

HSP 1 Score: 51.2174 bits (121), Expect = 2.607e-6
Identity = 79/408 (19.36%), Postives = 161/408 (39.46%), Query Frame = 3 
Query:  168 SCCTIPNENSLKSISTDDYLEIIRRLIKTNREKLNHLQNRFLEFHKAVIMIFANKF---DFLKILPMKSQLRNGDQMAVKLFENNLFSYINNIEITQSLEEVLTEFLAKLFQASVVNPCRENQDLYLSDY-----QNRKTRHRFHSKHRLSRIVLLQWVKRNGGSDLVQSLTKAIDMFVSEINKTKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFE-MIENDWKFG---------------IIGAIQTFYDELMRIILKL----------HIPDEFNKKDNRRCSMNSFLKYLTENATLYEESQRNASIHL---VYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNGSASSSY 1280
            +CCT   E +L ++S  ++  ++R   ++ +  LN  Q R  + +   ++  + ++    F+K L     L   + +  +   + L  Y     +  +LEE L EF A+L +  +         L   D+     +  +T   F    R                   +  T+ + +FV+     ++ V   N++    D + K     +  +C+  I+++ +C +C G     PC + C  ++  CL++   +   W  L + M++    FG               I  A+Q   + +     KL            P   +++  ++ S  + L+Y   +A         A++ L   V +  ++++  +  W  LA  +C N+   +  SN +KCWNG A + Y
Sbjct:   33 TCCTSAMEETLSNLSRREFEGLVREAGRSIQATLNA-QYRSFDTYFIELLNGSERWLEEAFVKAL---GALYGPNAVVFRDLYSELRRYYRGAAL--NLEEALDEFWARLLERMLKASDPAAASLISDDFLECASKQAETLRLFGEAPR-------------------ELKTRLVRVFVA----ARAFVQGLNMAG---DVVRKVSQVPLTPECNRAIMKLVYCPHCRGLGSVKPCANYCKNVMKGCLANQADLDTEWQNLIDTMLQVASSFGTEPSMDVILHSIPVRISEAVQHMQENMELYTNKLFQACGERGEEGTPTSVSEEPKKKESTVTTLEYKPSSA---------AAVRLEVQVSDVSSKLKEMQMFWIQLASALC-NSKAASGSSNNDKCWNGMAKARY 398
[Close]
BLAST of SMED30027246 vs. Ensembl Cavefish
Match: gpc1a (glypican 1a [Source:NCBI gene;Acc:553367])

HSP 1 Score: 51.2174 bits (121), Expect = 2.702e-6
Identity = 79/408 (19.36%), Postives = 161/408 (39.46%), Query Frame = 3 
Query:  168 SCCTIPNENSLKSISTDDYLEIIRRLIKTNREKLNHLQNRFLEFHKAVIMIFANKF---DFLKILPMKSQLRNGDQMAVKLFENNLFSYINNIEITQSLEEVLTEFLAKLFQASVVNPCRENQDLYLSDY-----QNRKTRHRFHSKHRLSRIVLLQWVKRNGGSDLVQSLTKAIDMFVSEINKTKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFE-MIENDWKFG---------------IIGAIQTFYDELMRIILKL----------HIPDEFNKKDNRRCSMNSFLKYLTENATLYEESQRNASIHL---VYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNGSASSSY 1280
            +CCT   E +L ++S  ++  ++R   ++ +  LN  Q R  + +   ++  + ++    F+K L     L   + +  +   + L  Y     +  +LEE L EF A+L +  +         L   D+     +  +T   F    R                   +  T+ + +FV+     ++ V   N++    D + K     +  +C+  I+++ +C +C G     PC + C  ++  CL++   +   W  L + M++    FG               I  A+Q   + +     KL            P   +++  ++ S  + L+Y   +A         A++ L   V +  ++++  +  W  LA  +C N+   +  SN +KCWNG A + Y
Sbjct:   61 TCCTSAMEETLSNLSRREFEGLVREAGRSIQATLNA-QYRSFDTYFIELLNGSERWLEEAFVKAL---GALYGPNAVVFRDLYSELRRYYRGAAL--NLEEALDEFWARLLERMLKASDPAAASLISDDFLECASKQAETLRLFGEAPR-------------------ELKTRLVRVFVA----ARAFVQGLNMAG---DVVRKVSQVPLTPECNRAIMKLVYCPHCRGLGSVKPCANYCKNVMKGCLANQADLDTEWQNLIDTMLQVASSFGTEPSMDVILHSIPVRISEAVQHMQENMELYTNKLFQACGERGEEGTPTSVSEEPKKKESTVTTLEYKPSSA---------AAVRLEVQVSDVSSKLKEMQMFWIQLASALC-NSKAASGSSNNDKCWNGMAKARY 426
[Close]
BLAST of SMED30027246 vs. Ensembl Cavefish
Match: gpc1a (glypican 1a [Source:NCBI gene;Acc:553367])

HSP 1 Score: 51.2174 bits (121), Expect = 2.818e-6
Identity = 79/408 (19.36%), Postives = 161/408 (39.46%), Query Frame = 3 
Query:  168 SCCTIPNENSLKSISTDDYLEIIRRLIKTNREKLNHLQNRFLEFHKAVIMIFANKF---DFLKILPMKSQLRNGDQMAVKLFENNLFSYINNIEITQSLEEVLTEFLAKLFQASVVNPCRENQDLYLSDY-----QNRKTRHRFHSKHRLSRIVLLQWVKRNGGSDLVQSLTKAIDMFVSEINKTKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFE-MIENDWKFG---------------IIGAIQTFYDELMRIILKL----------HIPDEFNKKDNRRCSMNSFLKYLTENATLYEESQRNASIHL---VYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNGSASSSY 1280
            +CCT   E +L ++S  ++  ++R   ++ +  LN  Q R  + +   ++  + ++    F+K L     L   + +  +   + L  Y     +  +LEE L EF A+L +  +         L   D+     +  +T   F    R                   +  T+ + +FV+     ++ V   N++    D + K     +  +C+  I+++ +C +C G     PC + C  ++  CL++   +   W  L + M++    FG               I  A+Q   + +     KL            P   +++  ++ S  + L+Y   +A         A++ L   V +  ++++  +  W  LA  +C N+   +  SN +KCWNG A + Y
Sbjct:   33 TCCTSAMEETLSNLSRREFEGLVREAGRSIQATLNA-QYRSFDTYFIELLNGSERWLEEAFVKAL---GALYGPNAVVFRDLYSELRRYYRGAAL--NLEEALDEFWARLLERMLKASDPAAASLISDDFLECASKQAETLRLFGEAPR-------------------ELKTRLVRVFVA----ARAFVQGLNMAG---DVVRKVSQVPLTPECNRAIMKLVYCPHCRGLGSVKPCANYCKNVMKGCLANQADLDTEWQNLIDTMLQVASSFGTEPSMDVILHSIPVRISEAVQHMQENMELYTNKLFQACGERGEEGTPTSVSEEPKKKESTVTTLEYKPSSA---------AAVRLEVQVSDVSSKLKEMQMFWIQLASALC-NSKAASGSSNNDKCWNGMAKARY 398
[Close]
BLAST of SMED30027246 vs. Ensembl Medaka
Match: gpc1b (glypican-1 [Source:NCBI gene;Acc:101173336])

HSP 1 Score: 51.9878 bits (123), Expect = 1.526e-6
Identity = 69/317 (21.77%), Postives = 124/317 (39.12%), Query Frame = 3 
Query:  384 QMAVKLFE---NNLFSYINNIEITQSLEEVLTEFLAKLFQASVVNPCRENQDLYLS-DYQNRKTRHRFHSKHRLSRIVLLQWVKRNGGSDLVQSLTKAIDMFVSEINKTKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIENDWKFGIIGAIQTFYDELMRIILKLHIPDE----FNKKDNRRCSMNSFLKYLTENA----------TLYEESQRNASIHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNGSASSSY 1280
            QM  ++F    N+L  Y     +  +LEEVL EF AKL +  V    ++N+   L  DY    ++        +  I       R    ++ ++L  A       +   + +  V  + +S                C   ++RM++C +C G     PC + C  ++  CL++   ++  W  L + +    +  +IG  QT      RI   ++   E     N K ++ C     +    E            TL + S+  A   LV      +R+ +S W +L +I+C +     + +  +KCWNG   + Y
Sbjct:  133 QMNTQVFSDLYNDLSGYCRGGSV--NLEEVLNEFWAKLLEKLVY---QKNKQFSLGEDYLECLSKQ-------IETIRPFGETPRKMSGEVTRTLLAARSFLQGLVKSAEVVRKVSQVPLSPA--------------CMRAMMRMTYCPHCRGIPSARPCANYCSNVMKGCLANQADLNTEWKHLVDTMVFVAENRLIGMDQTILSLPERISEAMYTMMEKIETINAKLSQACGEVGPISTGAEEVRTVGRVTVEDTLVDTSKNMA--KLVSEVTMRLRDMQSYWVSLPKILCSDRAAHGAGAE-DKCWNGINRARY 420
[Close]
BLAST of SMED30027246 vs. Planmine SMEST
Match: SMESG000047430.1 (SMESG000047430.1)

HSP 1 Score: 1097.03 bits (2836), Expect = 0.000e+0
Identity = 549/552 (99.46%), Postives = 552/552 (100.00%), Query Frame = 3 
Query:  102 VVSKSRHCLPIDSNSIGNGVELSCCTIPNENSLKSISTDDYLEIIRRLIKTNREKLNHLQNRFLEFHKAVIMIFANKFDFLKILPMKSQLRNGDQMAVKLFENNLFSYINNIEITQSLEEVLTEFLAKLFQASVVNPCRENQDLYLSDYQNRKTRHRFHSKHRLSRIVLLQWVKRNGGSDLVQSLTKAIDMFVSEINKTKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIENDWKFGIIGAIQTFYDELMRIILKLHIPDEFNKKDNRRCSMNSFLKYLTENATLYEESQRNASIHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNGSASSSYTWHQCSFCENTQKSKEISRISKNEILQTVIITLQNFTKTCQKNHKPSDRLLKKALPAFKMDVIEKNGNKYNETKINEFALKDLNPNHFMIISDNEKLVDNEEGSGTYNQVKFVNRNTQPIQSFNIKTSAITNNGVFSILYKGXXXXXXXXXXXXXLWKM 1757
            VVSKSRHCLPIDSNSIGNGVELSCCTIPNENSLKSISTDDYLEIIRRLIKTNREKL+HLQNRFLEFHKAVIMIFANKFDFLKILPMKSQLRNGDQMAVKLFENNLFSYINNIEITQSLEEVLTEFLAKLFQASVVNPCRENQDLYLSDYQNRKTRHRFHSKHRLSRIVLLQWVKRNGGSDLVQSLTKAIDMFVSEINKTKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIENDWKFGIIGAIQTFYDELMRIILKLHIP+EFN+KDNRRCSMNSFLKYLTENATLYEESQRNASIHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNGSASSSYTWHQCSFCENTQKSKEISRISKNEILQTVIITLQNFTKTCQKNHKPSDRLLKKALPAFKMDVIEKNGNKYNETKINEFALKDLNPNHFMIISDNEKLVDNEEGSGTYNQVKFVNRNTQPIQSFNIKTSAITNNGVFSILYKGFFSIFIIVFFQIILWKM
Sbjct:   51 VVSKSRHCLPIDSNSIGNGVELSCCTIPNENSLKSISTDDYLEIIRRLIKTNREKLSHLQNRFLEFHKAVIMIFANKFDFLKILPMKSQLRNGDQMAVKLFENNLFSYINNIEITQSLEEVLTEFLAKLFQASVVNPCRENQDLYLSDYQNRKTRHRFHSKHRLSRIVLLQWVKRNGGSDLVQSLTKAIDMFVSEINKTKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIENDWKFGIIGAIQTFYDELMRIILKLHIPEEFNQKDNRRCSMNSFLKYLTENATLYEESQRNASIHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNGSASSSYTWHQCSFCENTQKSKEISRISKNEILQTVIITLQNFTKTCQKNHKPSDRLLKKALPAFKMDVIEKNGNKYNETKINEFALKDLNPNHFMIISDNEKLVDNEEGSGTYNQVKFVNRNTQPIQSFNIKTSAITNNGVFSILYKGFFSIFIIVFFQIILWKM 602
[Close]
BLAST of SMED30027246 vs. Planmine SMEST
Match: SMESG000047430.1 (SMESG000047430.1)

HSP 1 Score: 1096.26 bits (2834), Expect = 0.000e+0
Identity = 549/552 (99.46%), Postives = 552/552 (100.00%), Query Frame = 3 
Query:  102 VVSKSRHCLPIDSNSIGNGVELSCCTIPNENSLKSISTDDYLEIIRRLIKTNREKLNHLQNRFLEFHKAVIMIFANKFDFLKILPMKSQLRNGDQMAVKLFENNLFSYINNIEITQSLEEVLTEFLAKLFQASVVNPCRENQDLYLSDYQNRKTRHRFHSKHRLSRIVLLQWVKRNGGSDLVQSLTKAIDMFVSEINKTKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIENDWKFGIIGAIQTFYDELMRIILKLHIPDEFNKKDNRRCSMNSFLKYLTENATLYEESQRNASIHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNGSASSSYTWHQCSFCENTQKSKEISRISKNEILQTVIITLQNFTKTCQKNHKPSDRLLKKALPAFKMDVIEKNGNKYNETKINEFALKDLNPNHFMIISDNEKLVDNEEGSGTYNQVKFVNRNTQPIQSFNIKTSAITNNGVFSILYKGXXXXXXXXXXXXXLWKM 1757
            VVSKSRHCLPIDSNSIGNGVELSCCTIPNENSLKSISTDDYLEIIRRLIKTNREKL+HLQNRFLEFHKAVIMIFANKFDFLKILPMKSQLRNGDQMAVKLFENNLFSYINNIEITQSLEEVLTEFLAKLFQASVVNPCRENQDLYLSDYQNRKTRHRFHSKHRLSRIVLLQWVKRNGGSDLVQSLTKAIDMFVSEINKTKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIENDWKFGIIGAIQTFYDELMRIILKLHIP+EFN+KDNRRCSMNSFLKYLTENATLYEESQRNASIHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNGSASSSYTWHQCSFCENTQKSKEISRISKNEILQTVIITLQNFTKTCQKNHKPSDRLLKKALPAFKMDVIEKNGNKYNETKINEFALKDLNPNHFMIISDNEKLVDNEEGSGTYNQVKFVNRNTQPIQSFNIKTSAITNNGVFSILYKGFFSIFIIVFFQIILWKM
Sbjct:   32 VVSKSRHCLPIDSNSIGNGVELSCCTIPNENSLKSISTDDYLEIIRRLIKTNREKLSHLQNRFLEFHKAVIMIFANKFDFLKILPMKSQLRNGDQMAVKLFENNLFSYINNIEITQSLEEVLTEFLAKLFQASVVNPCRENQDLYLSDYQNRKTRHRFHSKHRLSRIVLLQWVKRNGGSDLVQSLTKAIDMFVSEINKTKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIENDWKFGIIGAIQTFYDELMRIILKLHIPEEFNQKDNRRCSMNSFLKYLTENATLYEESQRNASIHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNGSASSSYTWHQCSFCENTQKSKEISRISKNEILQTVIITLQNFTKTCQKNHKPSDRLLKKALPAFKMDVIEKNGNKYNETKINEFALKDLNPNHFMIISDNEKLVDNEEGSGTYNQVKFVNRNTQPIQSFNIKTSAITNNGVFSILYKGFFSIFIIVFFQIILWKM 583
[Close]
BLAST of SMED30027246 vs. Planmine SMEST
Match: SMESG000047430.1 (SMESG000047430.1)

HSP 1 Score: 1028.85 bits (2659), Expect = 0.000e+0
Identity = 499/503 (99.20%), Postives = 503/503 (100.00%), Query Frame = 3 
Query:  102 VVSKSRHCLPIDSNSIGNGVELSCCTIPNENSLKSISTDDYLEIIRRLIKTNREKLNHLQNRFLEFHKAVIMIFANKFDFLKILPMKSQLRNGDQMAVKLFENNLFSYINNIEITQSLEEVLTEFLAKLFQASVVNPCRENQDLYLSDYQNRKTRHRFHSKHRLSRIVLLQWVKRNGGSDLVQSLTKAIDMFVSEINKTKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIENDWKFGIIGAIQTFYDELMRIILKLHIPDEFNKKDNRRCSMNSFLKYLTENATLYEESQRNASIHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNGSASSSYTWHQCSFCENTQKSKEISRISKNEILQTVIITLQNFTKTCQKNHKPSDRLLKKALPAFKMDVIEKNGNKYNETKINEFALKDLNPNHFMIISDNEKLVDNEEGSGTYNQV 1610
            VVSKSRHCLPIDSNSIGNGVELSCCTIPNENSLKSISTDDYLEIIRRLIKTNREKL+HLQNRFLEFHKAVIMIFANKFDFLKILPMKSQLRNGDQMAVKLFENNLFSYINNIEITQSLEEVLTEFLAKLFQASVVNPCRENQDLYLSDYQNRKTRHRFHSKHRLSRIVLLQWVKRNGGSDLVQSLTKAIDMFVSEINKTKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIENDWKFGIIGAIQTFYDELMRIILKLHIP+EFN+KDNRRCSMNSFLKYLTENATLYEESQRNASIHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNGSASSSYTWHQCSFCENTQKSKEISRISKNEILQTVIITLQNFTKTCQKNHKPSDRLLKKALPAFKMDVIEKNGNKYNETKINEFALKDLNPNHFMIISDNEKLVDNEEGSGTYNQ+
Sbjct:   32 VVSKSRHCLPIDSNSIGNGVELSCCTIPNENSLKSISTDDYLEIIRRLIKTNREKLSHLQNRFLEFHKAVIMIFANKFDFLKILPMKSQLRNGDQMAVKLFENNLFSYINNIEITQSLEEVLTEFLAKLFQASVVNPCRENQDLYLSDYQNRKTRHRFHSKHRLSRIVLLQWVKRNGGSDLVQSLTKAIDMFVSEINKTKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIENDWKFGIIGAIQTFYDELMRIILKLHIPEEFNQKDNRRCSMNSFLKYLTENATLYEESQRNASIHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNGSASSSYTWHQCSFCENTQKSKEISRISKNEILQTVIITLQNFTKTCQKNHKPSDRLLKKALPAFKMDVIEKNGNKYNETKINEFALKDLNPNHFMIISDNEKLVDNEEGSGTYNQL 534
[Close]
BLAST of SMED30027246 vs. Planmine SMEST
Match: SMESG000047430.1 (SMESG000047430.1)

HSP 1 Score: 1028.47 bits (2658), Expect = 0.000e+0
Identity = 499/503 (99.20%), Postives = 503/503 (100.00%), Query Frame = 3 
Query:  102 VVSKSRHCLPIDSNSIGNGVELSCCTIPNENSLKSISTDDYLEIIRRLIKTNREKLNHLQNRFLEFHKAVIMIFANKFDFLKILPMKSQLRNGDQMAVKLFENNLFSYINNIEITQSLEEVLTEFLAKLFQASVVNPCRENQDLYLSDYQNRKTRHRFHSKHRLSRIVLLQWVKRNGGSDLVQSLTKAIDMFVSEINKTKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIENDWKFGIIGAIQTFYDELMRIILKLHIPDEFNKKDNRRCSMNSFLKYLTENATLYEESQRNASIHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNGSASSSYTWHQCSFCENTQKSKEISRISKNEILQTVIITLQNFTKTCQKNHKPSDRLLKKALPAFKMDVIEKNGNKYNETKINEFALKDLNPNHFMIISDNEKLVDNEEGSGTYNQV 1610
            VVSKSRHCLPIDSNSIGNGVELSCCTIPNENSLKSISTDDYLEIIRRLIKTNREKL+HLQNRFLEFHKAVIMIFANKFDFLKILPMKSQLRNGDQMAVKLFENNLFSYINNIEITQSLEEVLTEFLAKLFQASVVNPCRENQDLYLSDYQNRKTRHRFHSKHRLSRIVLLQWVKRNGGSDLVQSLTKAIDMFVSEINKTKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIENDWKFGIIGAIQTFYDELMRIILKLHIP+EFN+KDNRRCSMNSFLKYLTENATLYEESQRNASIHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNGSASSSYTWHQCSFCENTQKSKEISRISKNEILQTVIITLQNFTKTCQKNHKPSDRLLKKALPAFKMDVIEKNGNKYNETKINEFALKDLNPNHFMIISDNEKLVDNEEGSGTYNQ+
Sbjct:   51 VVSKSRHCLPIDSNSIGNGVELSCCTIPNENSLKSISTDDYLEIIRRLIKTNREKLSHLQNRFLEFHKAVIMIFANKFDFLKILPMKSQLRNGDQMAVKLFENNLFSYINNIEITQSLEEVLTEFLAKLFQASVVNPCRENQDLYLSDYQNRKTRHRFHSKHRLSRIVLLQWVKRNGGSDLVQSLTKAIDMFVSEINKTKSLVNVFNISISILDSLYKCQDCFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISELWIKLFEMIENDWKFGIIGAIQTFYDELMRIILKLHIPEEFNQKDNRRCSMNSFLKYLTENATLYEESQRNASIHLVYNACTEVRNQKSLWGNLAEIVCKNAPWFTSLSNTNKCWNGSASSSYTWHQCSFCENTQKSKEISRISKNEILQTVIITLQNFTKTCQKNHKPSDRLLKKALPAFKMDVIEKNGNKYNETKINEFALKDLNPNHFMIISDNEKLVDNEEGSGTYNQL 553
The following BLAST results are available for this feature:
BLAST of SMED30027246 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
GPC51.637e-621.96glypican 5 [Source:HGNC Symbol;Acc:HGNC:4453][more]
GPC56.867e-622.07glypican 5 [Source:HGNC Symbol;Acc:HGNC:4453][more]
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BLAST of SMED30027246 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027246 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027246 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027246 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027246 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027246 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|P50593|GPC1_CHICK3.082e-623.20Glypican-1 OS=Gallus gallus OX=9031 GN=GPC1 PE=2 S... [more]
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BLAST of SMED30027246 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4S2LLC71.363e-1023.11Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A4S2LLC91.397e-1023.11Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A075AHD51.686e-923.76Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A1A9W3G97.503e-723.31Uncharacterized protein OS=Glossina brevipalpis OX... [more]
A0A437CFM08.861e-722.47Uncharacterized protein OS=Oryzias javanicus OX=12... [more]
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BLAST of SMED30027246 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
gpc44.916e-819.27glypican-4 [Source:NCBI gene;Acc:103025650][more]
gpc1a2.607e-619.36glypican 1a [Source:NCBI gene;Acc:553367][more]
gpc1a2.702e-619.36glypican 1a [Source:NCBI gene;Acc:553367][more]
gpc1a2.818e-619.36glypican 1a [Source:NCBI gene;Acc:553367][more]
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BLAST of SMED30027246 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027246 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027246 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027246 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gpc1b1.526e-621.77glypican-1 [Source:NCBI gene;Acc:101173336][more]
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BLAST of SMED30027246 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000047430.10.000e+099.46SMESG000047430.1[more]
SMESG000047430.10.000e+099.46SMESG000047430.1[more]
SMESG000047430.10.000e+099.20SMESG000047430.1[more]
SMESG000047430.10.000e+099.20SMESG000047430.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027246 ID=SMED30027246|Name=SMED30027246|organism=Schmidtea mediterranea sexual|type=transcript|length=1898bp
GGTAGCATGTCTATGAAAAATAATTCCTCCATCATTTTATTGGCGTCATT
GATTATTGCCATCTATGTTTCTAATTAATGGTGAAGCAATTTTAATTTTT
AGTCGTTTCGAAATCCCGACATTGTCTGCCAATCGATTCCAATTCCATTG
GCAATGGCGTGGAGCTCTCGTGTTGTACGATTCCAAATGAAAATTCTCTC
AAATCAATATCAACTGATGATTATTTGGAAATTATTCGCAGACTTATCAA
AACTAACCGAGAAAAGTTGAATCATTTACAAAACAGATTTTTGGAGTTCC
ATAAAGCTGTTATAATGATATTTGCAAATAAATTTGACTTTCTCAAAATT
CTGCCAATGAAATCTCAGCTGCGCAATGGTGACCAAATGGCGGTGAAATT
GTTTGAAAATAACCTATTTTCATACATTAACAACATAGAAATTACTCAAT
CCCTAGAAGAAGTTTTAACAGAATTTTTAGCTAAACTCTTCCAAGCTTCT
GTTGTTAATCCATGTAGAGAAAACCAGGATCTCTATCTCTCCGATTATCA
AAATCGAAAAACCAGGCACAGATTCCATTCAAAGCATCGGTTATCTCGAA
TTGTCCTATTACAGTGGGTCAAAAGAAACGGAGGTTCCGATCTCGTTCAA
TCTCTCACAAAAGCTATTGATATGTTTGTTTCCGAAATCAACAAGACAAA
ATCCTTAGTTAATGTTTTCAATATATCCATTTCAATTCTAGACTCTTTAT
ACAAATGTCAAGATTGTTTTATTCAAAACGATTGCTCAATTAATATACTT
AGAATGTCACATTGCGCTTATTGTGCAGGAAAAGTTTTTACAATTCCATG
TTTCTCTGTGTGCTTACAAATTATTAATAAATGTTTATCAGATCTCCAAA
TTATCTCAGAATTATGGATCAAACTATTCGAAATGATAGAAAATGATTGG
AAATTTGGAATTATTGGCGCCATTCAGACATTTTATGATGAGTTGATGAG
AATTATTTTGAAACTTCATATTCCCGACGAATTCAATAAAAAAGACAACA
GAAGATGTTCAATGAACTCTTTTTTAAAGTATCTGACGGAAAATGCGACA
CTATACGAAGAATCTCAACGAAATGCAAGTATACATCTAGTATATAATGC
TTGTACTGAGGTAAGAAATCAAAAATCACTTTGGGGAAATCTGGCGGAAA
TTGTGTGTAAAAACGCTCCATGGTTCACTTCACTTTCCAATACAAATAAA
TGCTGGAATGGAAGTGCATCATCAAGTTATACATGGCATCAATGCTCATT
TTGTGAAAACACTCAAAAGTCAAAAGAAATCTCAAGAATCAGTAAAAATG
AAATTTTACAAACAGTTATTATAACTCTGCAAAATTTCACTAAAACCTGT
CAAAAAAATCATAAACCTTCAGATCGACTTCTAAAGAAAGCATTACCCGC
TTTCAAAATGGATGTAATAGAAAAAAATGGAAATAAATATAATGAAACGA
AAATAAATGAGTTTGCTTTAAAAGATTTAAATCCAAACCATTTCATGATC
ATTTCCGACAATGAAAAATTAGTGGACAATGAGGAAGGTTCAGGGACATA
CAACCAAGTAAAATTTGTTAATAGAAATACCCAACCAATTCAAAGCTTTA
ATATAAAAACATCTGCTATCACAAACAATGGCGTCTTTTCCATATTATAT
AAAGGATTTTTCTCTATTTTCATCATTGTATTTTTTCAAATAATTTTATG
GAAAATGTAAATTTTTTTAATGTAATGTAGTTAGCATTACTTGGCTTGAT
ATGTCTATATTAATTTTATTCGGTAATGAGCATATTTTATTTTCTGATTA
GTCAAAGGTTCATTTTTCAACGAATTCTGAAGGTATGTAAAGTTTGGG
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protein sequence of SMED30027246-orf-1

>SMED30027246-orf-1 ID=SMED30027246-orf-1|Name=SMED30027246-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=482bp
MIFANKFDFLKILPMKSQLRNGDQMAVKLFENNLFSYINNIEITQSLEEV
LTEFLAKLFQASVVNPCRENQDLYLSDYQNRKTRHRFHSKHRLSRIVLLQ
WVKRNGGSDLVQSLTKAIDMFVSEINKTKSLVNVFNISISILDSLYKCQD
CFIQNDCSINILRMSHCAYCAGKVFTIPCFSVCLQIINKCLSDLQIISEL
WIKLFEMIENDWKFGIIGAIQTFYDELMRIILKLHIPDEFNKKDNRRCSM
NSFLKYLTENATLYEESQRNASIHLVYNACTEVRNQKSLWGNLAEIVCKN
APWFTSLSNTNKCWNGSASSSYTWHQCSFCENTQKSKEISRISKNEILQT
VIITLQNFTKTCQKNHKPSDRLLKKALPAFKMDVIEKNGNKYNETKINEF
ALKDLNPNHFMIISDNEKLVDNEEGSGTYNQVKFVNRNTQPIQSFNIKTS
AITNNGVFSILYKGFFSIFIIVFFQIILWKM*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000421tail
Vocabulary: INTERPRO
TermDefinition
IPR031187Dally
IPR001863Glypican
Vocabulary: biological process
TermDefinition
GO:0009966regulation of signal transduction
GO:0090263positive regulation of canonical Wnt signaling pathway
GO:0045880positive regulation of smoothened signaling pathway
GO:0030513positive regulation of BMP signaling pathway
Vocabulary: cellular component
TermDefinition
GO:0046658anchored component of plasma membrane
GO:0005578proteinaceous extracellular matrix
GO:0016020membrane
Vocabulary: molecular function
TermDefinition
GO:0043395heparan sulfate proteoglycan binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001863GlypicanPFAMPF01153Glypicancoord: 266..363
e-value: 3.9E-7
score: 29.3
coord: 128..206
e-value: 1.0E-6
score: 27.9
IPR001863GlypicanPANTHERPTHR10822GLYPICANcoord: 122..446
IPR031187Division abnormally delayed proteinPANTHERPTHR10822:SF29DIVISION ABNORMALLY DELAYED PROTEINcoord: 122..446
NoneNo IPR availableTMHMMTMhelixcoord: 457..479