Calcium dependent protein kinase 5

Overview
NameCalcium dependent protein kinase 5
Smed IDSMED30027240
Length (bp)637
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Calcium dependent protein kinase 5 (SMED30027240) t-SNE clustered cells

Violin plots show distribution of expression levels for Calcium dependent protein kinase 5 (SMED30027240) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Calcium dependent protein kinase 5 (SMED30027240) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Calcium dependent protein kinase 5 (SMED30027240) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30027240

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30027240SMESG000057759.1 SmedASXL_004251SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30027240SMESG000057759.1 dd_Smed_v4_15455_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30027240SMESG000057759.1 dd_Smed_v4_15455_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30027240SMESG000057759.1 dd_Smed_v4_15455_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30027240SMESG000057759.1 dd_15455dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult fluorescence in situ hybridization evidence
head regionSMED30027240SMESG000057759.1 dd_Smed_v6_15455_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
head regionSMED30027240SMESG000057759.1 OX_Smed_1.0.15213ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Human
Match: PPP3R2 (protein phosphatase 3 regulatory subunit B, beta [Source:HGNC Symbol;Acc:HGNC:9318])

HSP 1 Score: 48.1358 bits (113), Expect = 1.711e-6
Identity = 30/94 (31.91%), Postives = 51/94 (54.26%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNE-VEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQ 477
            F  +D DKSG++ + E + +   +H   +  ++  ID FD + DG++ FKEFI    +  +K     +    F   D D +GYI+ GELF +++
Sbjct:   27 FKKLDLDKSGSLSVEEFMSLPELRH---NPLVRRVIDVFDTDGDGEVDFKEFILGTSQFSVKGDEEQKLRFAFSIYDMDKDGYISNGELFQVLK 117          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Human
Match: PPP3R1 (protein phosphatase 3 regulatory subunit B, alpha [Source:HGNC Symbol;Acc:HGNC:9317])

HSP 1 Score: 47.7506 bits (112), Expect = 1.755e-6
Identity = 33/112 (29.46%), Postives = 57/112 (50.89%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SG++ + E          Q+  ++  ID FD + +G++ FKEFI  + +  +K     +    F+  D D +GYI+ GELF +++     +++K  QL   V K
Sbjct:   17 FKKLDLDNSGSLSVEEFMSLPELQ--QNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVG-NNLKDTQLQQIVDK 125          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Human
Match: PPP3R2 (protein phosphatase 3 regulatory subunit B, beta [Source:HGNC Symbol;Acc:HGNC:9318])

HSP 1 Score: 48.1358 bits (113), Expect = 1.794e-6
Identity = 30/94 (31.91%), Postives = 51/94 (54.26%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNE-VEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQ 477
            F  +D DKSG++ + E + +   +H   +  ++  ID FD + DG++ FKEFI    +  +K     +    F   D D +GYI+ GELF +++
Sbjct:   30 FKKLDLDKSGSLSVEEFMSLPELRH---NPLVRRVIDVFDTDGDGEVDFKEFILGTSQFSVKGDEEQKLRFAFSIYDMDKDGYISNGELFQVLK 120          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Human
Match: PPP3R1 (protein phosphatase 3 regulatory subunit B, alpha [Source:HGNC Symbol;Acc:HGNC:9317])

HSP 1 Score: 47.7506 bits (112), Expect = 2.103e-6
Identity = 33/112 (29.46%), Postives = 57/112 (50.89%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SG++ + E          Q+  ++  ID FD + +G++ FKEFI  + +  +K     +    F+  D D +GYI+ GELF +++     +++K  QL   V K
Sbjct:   27 FKKLDLDNSGSLSVEEFMSLPELQ--QNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVG-NNLKDTQLQQIVDK 135          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Human
Match: PPP3R1 (protein phosphatase 3 regulatory subunit B, alpha [Source:HGNC Symbol;Acc:HGNC:9317])

HSP 1 Score: 47.7506 bits (112), Expect = 2.256e-6
Identity = 33/112 (29.46%), Postives = 57/112 (50.89%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SG++ + E          Q+  ++  ID FD + +G++ FKEFI  + +  +K     +    F+  D D +GYI+ GELF +++     +++K  QL   V K
Sbjct:   46 FKKLDLDNSGSLSVEEFMSLPELQ--QNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVG-NNLKDTQLQQIVDK 154          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Celegans
Match: M04F3.4 (pep chromosome:WBcel235:I:4771348:4773070:-1 gene:WBGene00019770.1 transcript:M04F3.4b.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:M04F3.4)

HSP 1 Score: 54.299 bits (129), Expect = 3.110e-9
Identity = 34/99 (34.34%), Postives = 48/99 (48.48%), Query Frame = 1
Query:  193 QIFYDIDKDKSGAIELNEVEIYLRK---HGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQE 480
            QIF  +DKD+SG I  +E++  L     + F  E  +L I  FD N DG I F+EF           R   +W + F+  D D +G I   EL N + +
Sbjct:   10 QIFSSVDKDRSGQISSDELQTALSNGTWNPFNPETCRLMIGMFDSNGDGAINFQEF-------QALWRYINDWTNCFRGFDTDGSGNIDKSELTNALTQ 101          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Celegans
Match: M04F3.4 (pep chromosome:WBcel235:I:4771348:4773673:-1 gene:WBGene00019770.1 transcript:M04F3.4b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:M04F3.4)

HSP 1 Score: 54.299 bits (129), Expect = 3.110e-9
Identity = 34/99 (34.34%), Postives = 48/99 (48.48%), Query Frame = 1
Query:  193 QIFYDIDKDKSGAIELNEVEIYLRK---HGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQE 480
            QIF  +DKD+SG I  +E++  L     + F  E  +L I  FD N DG I F+EF           R   +W + F+  D D +G I   EL N + +
Sbjct:   10 QIFSSVDKDRSGQISSDELQTALSNGTWNPFNPETCRLMIGMFDSNGDGAINFQEF-------QALWRYINDWTNCFRGFDTDGSGNIDKSELTNALTQ 101          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Celegans
Match: M04F3.4 (pep chromosome:WBcel235:I:4771600:4773026:-1 gene:WBGene00019770.1 transcript:M04F3.4a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:M04F3.4)

HSP 1 Score: 54.299 bits (129), Expect = 3.110e-9
Identity = 34/99 (34.34%), Postives = 48/99 (48.48%), Query Frame = 1
Query:  193 QIFYDIDKDKSGAIELNEVEIYLRK---HGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQE 480
            QIF  +DKD+SG I  +E++  L     + F  E  +L I  FD N DG I F+EF           R   +W + F+  D D +G I   EL N + +
Sbjct:   10 QIFSSVDKDRSGQISSDELQTALSNGTWNPFNPETCRLMIGMFDSNGDGAINFQEF-------QALWRYINDWTNCFRGFDTDGSGNIDKSELTNALTQ 101          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Celegans
Match: F43C9.2 (pep chromosome:WBcel235:X:4786118:4790323:1 gene:WBGene00018376.1 transcript:F43C9.2a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F43C9.2)

HSP 1 Score: 51.2174 bits (121), Expect = 3.681e-8
Identity = 36/123 (29.27%), Postives = 63/123 (51.22%), Query Frame = 1
Query:  130 WIYSP---KITTRKYLMETEEEFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKL-FI-DTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQES 483
             I SP    I  ++Y+++ + +  + F  +D DK G +  NE+   LR    +   ++L FI    D +K GKI+ +EF+N +K  P+      E    F++ D D +G IT  E+  I++ +
Sbjct:    3 AIASPVNHDIICQRYVLD-DLQLSETFDLLDVDKDGRLSRNEIAALLRTINVEPTRVELDFIFGEMDTDKTGKISKEEFVNYMKSPPIHRTTLRELEVQFRKFDSDGDGAITEDEMAEILRRT 124          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Celegans
Match: F43C9.2 (pep chromosome:WBcel235:X:4783802:4790318:1 gene:WBGene00018376.1 transcript:F43C9.2b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F43C9.2)

HSP 1 Score: 50.8322 bits (120), Expect = 3.744e-8
Identity = 32/105 (30.48%), Postives = 54/105 (51.43%), Query Frame = 1
Query:  175 TEEEFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKL-FI-DTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQES 483
            T ++  + F  +D DK G +  NE+   LR    +   ++L FI    D +K GKI+ +EF+N +K  P+      E    F++ D D +G IT  E+  I++ +
Sbjct:    5 TPDQLSETFDLLDVDKDGRLSRNEIAALLRTINVEPTRVELDFIFGEMDTDKTGKISKEEFVNYMKSPPIHRTTLRELEVQFRKFDSDGDGAITEDEMAEILRRT 109          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Fly
Match: CanB2 (gene:FBgn0015614 transcript:FBtr0309866)

HSP 1 Score: 52.373 bits (124), Expect = 1.951e-8
Identity = 34/112 (30.36%), Postives = 60/112 (53.57%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SGA+ ++E          Q+  ++  ID FD + +G++ FKEFI  + +  +K    ++    F+  D D++GYI+ GELF +++     +++K  QL   V K
Sbjct:   27 FRKLDLDNSGALSVDEFMSLPELQ--QNPLVQRVIDIFDADGNGEVDFKEFIQGVSQFSVKGDKLSKLRFAFRIYDMDNDGYISNGELFQVLKMM-VGNNLKDTQLQQIVDK 135          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Fly
Match: CanB2 (gene:FBgn0015614 transcript:FBtr0088930)

HSP 1 Score: 52.373 bits (124), Expect = 1.951e-8
Identity = 34/112 (30.36%), Postives = 60/112 (53.57%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SGA+ ++E          Q+  ++  ID FD + +G++ FKEFI  + +  +K    ++    F+  D D++GYI+ GELF +++     +++K  QL   V K
Sbjct:   27 FRKLDLDNSGALSVDEFMSLPELQ--QNPLVQRVIDIFDADGNGEVDFKEFIQGVSQFSVKGDKLSKLRFAFRIYDMDNDGYISNGELFQVLKMM-VGNNLKDTQLQQIVDK 135          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Fly
Match: CanB (gene:FBgn0010014 transcript:FBtr0070764)

HSP 1 Score: 51.9878 bits (123), Expect = 3.146e-8
Identity = 33/112 (29.46%), Postives = 60/112 (53.57%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SGA+ ++E          Q+  ++  ID FD + +G++ FKEFI  + +  ++    ++    F+  D D++GYI+ GELF +++     +++K  QL   V K
Sbjct:   27 FRKLDLDNSGALSIDEFMSLPELQ--QNPLVQRVIDIFDADGNGEVDFKEFIQGVSQFSVRGDKLSKLRFAFRIYDMDNDGYISNGELFQVLKMM-VGNNLKDTQLQQIVDK 135          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Fly
Match: CanB (gene:FBgn0010014 transcript:FBtr0308348)

HSP 1 Score: 51.9878 bits (123), Expect = 3.146e-8
Identity = 33/112 (29.46%), Postives = 60/112 (53.57%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SGA+ ++E          Q+  ++  ID FD + +G++ FKEFI  + +  ++    ++    F+  D D++GYI+ GELF +++     +++K  QL   V K
Sbjct:   27 FRKLDLDNSGALSIDEFMSLPELQ--QNPLVQRVIDIFDADGNGEVDFKEFIQGVSQFSVRGDKLSKLRFAFRIYDMDNDGYISNGELFQVLKMM-VGNNLKDTQLQQIVDK 135          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Fly
Match: azot (gene:FBgn0033238 transcript:FBtr0088853)

HSP 1 Score: 48.521 bits (114), Expect = 4.382e-7
Identity = 31/97 (31.96%), Postives = 46/97 (47.42%), Query Frame = 1
Query:  187 FLQIFYDIDKDKSGAIELNEVEIYLRKHGFQS--EFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNI 471
             L  F  +DKD  GAI   E+ + +R  G Q     ++  I+  D   +G I   EF N + +      +  E    F+  D+D+NGYIT  EL N+
Sbjct:   12 ILDTFRILDKDNEGAITSKEMAVVIRALGRQPNDAEVQSMINEVDSEGNGSIVAPEFCNVILRKMRDTNHEDELREAFRIFDKDNNGYITTTELKNV 108          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Zebrafish
Match: ppp3r1a (protein phosphatase 3, regulatory subunit B, alpha a [Source:ZFIN;Acc:ZDB-GENE-081113-3])

HSP 1 Score: 48.1358 bits (113), Expect = 1.067e-6
Identity = 33/112 (29.46%), Postives = 57/112 (50.89%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SG++ + E          Q+  ++  ID FD + +G++ FKEFI  + +  +K     +    F+  D D +GYI+ GELF +++     +++K  QL   V K
Sbjct:   27 FKKLDLDNSGSLSVEEFMSLPELQ--QNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEMKLRFAFRIYDMDKDGYISNGELFQVLKMMVG-NNLKDTQLQQIVDK 135          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Zebrafish
Match: ppp3r1b (protein phosphatase 3 (formerly 2B), regulatory s1ubunit B, alpha isoform, b [Source:ZFIN;Acc:ZDB-GENE-040912-18])

HSP 1 Score: 47.7506 bits (112), Expect = 1.338e-6
Identity = 33/112 (29.46%), Postives = 57/112 (50.89%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SG++ + E          Q+  ++  ID FD + +G++ FKEFI  + +  +K     +    F+  D D +GYI+ GELF +++     +++K  QL   V K
Sbjct:   27 FKKLDLDNSGSLSVEEFMSLPELQ--QNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVG-NNLKDTQLQQIVDK 135          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Zebrafish
Match: cetn2 (centrin, EF-hand protein, 2 [Source:ZFIN;Acc:ZDB-GENE-091118-94])

HSP 1 Score: 46.595 bits (109), Expect = 3.306e-6
Identity = 31/105 (29.52%), Postives = 53/105 (50.48%), Query Frame = 1
Query:  172 ETEEEFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQS--EFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQE 480
            E ++E  + F   D D SG IE+ E+++ +R  GF+   E IK  I   D    GKI+F +F++ + +   +  +  E    F+  D+D  G I+   L  + +E
Sbjct:   28 EQKQEIREAFELFDTDGSGYIEVKELKVAMRALGFEPKKEEIKKMIAEVDKEATGKISFTDFLSVMTQKMAEKDSKEEILKAFRLFDDDETGKISFRNLKRVAKE 132          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Zebrafish
Match: ncaldb (neurocalcin delta b [Source:NCBI gene;Acc:445319])

HSP 1 Score: 46.595 bits (109), Expect = 3.973e-6
Identity = 37/105 (35.24%), Postives = 55/105 (52.38%), Query Frame = 1
Query:  175 TEEEFLQIFYDIDKD-KSGAIELNEVE-IYLR--KHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQ 477
            TE E L+ +    +D  SGA+ ++E + IY     +G  S+F +    TFD N DG I F+EFI +L  +  + R   +    F   D D NGYI+  E+  I+Q
Sbjct:   23 TEHEILEWYKGFLRDCPSGALSMDEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFIIAL-SVTSRGRLDQKLKWAFSMYDLDGNGYISKAEMLEIVQ 126          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Zebrafish
Match: ncaldb (neurocalcin delta b [Source:NCBI gene;Acc:445319])

HSP 1 Score: 46.595 bits (109), Expect = 3.973e-6
Identity = 37/105 (35.24%), Postives = 55/105 (52.38%), Query Frame = 1
Query:  175 TEEEFLQIFYDIDKD-KSGAIELNEVE-IYLR--KHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQ 477
            TE E L+ +    +D  SGA+ ++E + IY     +G  S+F +    TFD N DG I F+EFI +L  +  + R   +    F   D D NGYI+  E+  I+Q
Sbjct:   23 TEHEILEWYKGFLRDCPSGALSMDEFKKIYGNFFPYGDASKFAEHVFRTFDANGDGTIDFREFIIAL-SVTSRGRLDQKLKWAFSMYDLDGNGYISKAEMLEIVQ 126          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Xenopus
Match: hspe1 (heat shock protein family E (Hsp10) member 1 [Source:Xenbase;Acc:XB-GENE-963412])

HSP 1 Score: 47.7506 bits (112), Expect = 1.906e-6
Identity = 33/112 (29.46%), Postives = 57/112 (50.89%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SG++ + E          Q+  ++  ID FD + +G++ FKEFI  + +  +K     +    F+  D D +GYI+ GELF +++     +++K  QL   V K
Sbjct:   27 FKKLDLDNSGSLSVEEFMSLPELQ--QNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVG-NNLKDTQLQQIVDK 135          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Xenopus
Match: cetn1 (centrin 1 [Source:NCBI gene;Acc:549903])

HSP 1 Score: 46.595 bits (109), Expect = 4.291e-6
Identity = 31/118 (26.27%), Postives = 59/118 (50.00%), Query Frame = 1
Query:  133 IYSPKITTRKYLMETEEEFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQS--EFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQE 480
            +  P++T      E ++E  + F   D D +G I++ E+++ +R  GF+   E IK  I   D    GKI+F +F++++ +   +  +  E    F+  D+D  G I+   L  + +E
Sbjct:    5 VPKPELTE-----EQKQEIREAFDLFDTDGAGTIDVKELKVAMRALGFEPKKEEIKKMIADIDKEGTGKISFGDFMSAMTQKMAEKDSKEEIMKAFRLFDDDETGKISFKNLKRVAKE 117          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Xenopus
Match: cetn1 (centrin 1 [Source:NCBI gene;Acc:549903])

HSP 1 Score: 46.595 bits (109), Expect = 5.400e-6
Identity = 31/118 (26.27%), Postives = 59/118 (50.00%), Query Frame = 1
Query:  133 IYSPKITTRKYLMETEEEFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQS--EFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQE 480
            +  P++T      E ++E  + F   D D +G I++ E+++ +R  GF+   E IK  I   D    GKI+F +F++++ +   +  +  E    F+  D+D  G I+   L  + +E
Sbjct:   20 VPKPELTE-----EQKQEIREAFDLFDTDGAGTIDVKELKVAMRALGFEPKKEEIKKMIADIDKEGTGKISFGDFMSAMTQKMAEKDSKEEIMKAFRLFDDDETGKISFKNLKRVAKE 132          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Mouse
Match: Calm4 (calmodulin 4 [Source:MGI Symbol;Acc:MGI:1931464])

HSP 1 Score: 58.9214 bits (141), Expect = 8.079e-11
Identity = 46/138 (33.33%), Postives = 79/138 (57.25%), Query Frame = 1
Query:  184 EFLQIFYDIDKDKSGAIELNEVEIYLRKHG--FQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNK--HSIKKKQLSDFVQKXXXXXXXXXXYNEFLKF 585
            EF   F   DK+K G I + E+   +++ G     + +K  I   D + DGKI+F+EF+ +++K    HR   E  ++F  LD++ +GYIT+ EL    +ES +K   S+ +++L D ++  D+D DG + Y EF++ 
Sbjct:   12 EFQAAFNRFDKNKDGHISVEELGDVMKQLGKNLPEKDLKALISKLDTDGDGKISFEEFLTAIEKYKKGHR-AGELRAVFNVLDQNGDGYITVDEL----KESLSKLGESLSQEELEDMIRVADVDQDGKVKYEEFVRL 144          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Mouse
Match: Cetn1 (centrin 1 [Source:MGI Symbol;Acc:MGI:1347086])

HSP 1 Score: 48.1358 bits (113), Expect = 1.050e-6
Identity = 42/154 (27.27%), Postives = 77/154 (50.00%), Query Frame = 1
Query:  136 YSPKITTRKYLMETEEEFLQIFYDI-DKDKSGAIELNEVEIYLRKHGFQS--EFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQKXXXXXXXXXXYNEFLKFI 588
            Y  K+  +  L E +++ ++  +D+ D D SG I++ E+++ +R  GF+   E +K  I   D    GKI+F +F+  + +   +     E    F+  D+D  G I+   L  +  E     S+  ++L + + + D D DG +N  EFLK +
Sbjct:   15 YKRKVGPKPELTEDQKQEVREAFDLFDSDGSGTIDVKELKVAMRALGFEPRKEEMKKMISEVDKEATGKISFNDFLAVMTQKMAEKDTKEEILKAFRLFDDDETGKISFKNLKRVANEL--GESLTDEELQEMIDEADRDGDGEVNEEEFLKIM 166          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Mouse
Match: Cetn1 (centrin 1 [Source:MGI Symbol;Acc:MGI:1347086])

HSP 1 Score: 48.1358 bits (113), Expect = 1.050e-6
Identity = 42/154 (27.27%), Postives = 77/154 (50.00%), Query Frame = 1
Query:  136 YSPKITTRKYLMETEEEFLQIFYDI-DKDKSGAIELNEVEIYLRKHGFQS--EFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQKXXXXXXXXXXYNEFLKFI 588
            Y  K+  +  L E +++ ++  +D+ D D SG I++ E+++ +R  GF+   E +K  I   D    GKI+F +F+  + +   +     E    F+  D+D  G I+   L  +  E     S+  ++L + + + D D DG +N  EFLK +
Sbjct:   15 YKRKVGPKPELTEDQKQEVREAFDLFDSDGSGTIDVKELKVAMRALGFEPRKEEMKKMISEVDKEATGKISFNDFLAVMTQKMAEKDTKEEILKAFRLFDDDETGKISFKNLKRVANEL--GESLTDEELQEMIDEADRDGDGEVNEEEFLKIM 166          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Mouse
Match: Cetn1 (centrin 1 [Source:MGI Symbol;Acc:MGI:1347086])

HSP 1 Score: 48.1358 bits (113), Expect = 1.050e-6
Identity = 42/154 (27.27%), Postives = 77/154 (50.00%), Query Frame = 1
Query:  136 YSPKITTRKYLMETEEEFLQIFYDI-DKDKSGAIELNEVEIYLRKHGFQS--EFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQKXXXXXXXXXXYNEFLKFI 588
            Y  K+  +  L E +++ ++  +D+ D D SG I++ E+++ +R  GF+   E +K  I   D    GKI+F +F+  + +   +     E    F+  D+D  G I+   L  +  E     S+  ++L + + + D D DG +N  EFLK +
Sbjct:   15 YKRKVGPKPELTEDQKQEVREAFDLFDSDGSGTIDVKELKVAMRALGFEPRKEEMKKMISEVDKEATGKISFNDFLAVMTQKMAEKDTKEEILKAFRLFDDDETGKISFKNLKRVANEL--GESLTDEELQEMIDEADRDGDGEVNEEEFLKIM 166          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Mouse
Match: Ppp3r1 (protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I) [Source:MGI Symbol;Acc:MGI:107172])

HSP 1 Score: 47.7506 bits (112), Expect = 1.443e-6
Identity = 33/112 (29.46%), Postives = 57/112 (50.89%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SG++ + E          Q+  ++  ID FD + +G++ FKEFI  + +  +K     +    F+  D D +GYI+ GELF +++     +++K  QL   V K
Sbjct:   27 FKKLDLDNSGSLSVEEFMSLPELQ--QNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVG-NNLKDTQLQQIVDK 135          
BLAST of Calcium dependent protein kinase 5 vs. UniProt/SwissProt
Match: sp|Q07167|SM16_SCHMA (16 kDa calcium-binding protein OS=Schistosoma mansoni OX=6183 PE=2 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 3.307e-17
Identity = 44/140 (31.43%), Postives = 84/140 (60.00%), Query Frame = 1
Query:  169 METEEEFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQKXXXXXXXXXXYNEFLKFI 588
            M  E+++ ++F+ IDKDK+G +   E+   L++ G         I   D+N DGKI+ +E++N+L+K+P + +    W  +F+ +D+D +G ++I EL   ++ S     I +  L +++ +ND + DG L+Y+EFL ++
Sbjct:    1 MSEEKQWTEVFHAIDKDKNGFLTREEIAQCLKEVGVCPNVADKIIKETDMNSDGKISLEEYLNALRKLPPREKCVARWREVFQSIDKDGSGKVSIKELDEFLKTS--GMDIDQNSLRNWMTQNDKNKDGELDYDEFLAYV 138          
BLAST of Calcium dependent protein kinase 5 vs. UniProt/SwissProt
Match: sp|P05933|CALM_SCHPO (Calmodulin OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=cam1 PE=1 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 1.164e-10
Identity = 46/151 (30.46%), Postives = 79/151 (52.32%), Query Frame = 1
Query:  148 ITTRKYLMETEEEFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKL--FIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQKXXXXXXXXXXYNEFLKFIGT 594
            +TTR    E   EF + F   D+D+ G I  NE+ + +R  G      +L   I+  D + +G I F EF+  + +      N  E    FK  D+D NGYIT+ EL +++     +  + +++++D +++ D D DG++NY EF + I +
Sbjct:    1 MTTRNLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINEVDADGNGTIDFTEFLTMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGER--LSQEEVADMIREADTDGDGVINYEEFSRVISS 149          
BLAST of Calcium dependent protein kinase 5 vs. UniProt/SwissProt
Match: sp|Q9JM83|CALM4_MOUSE (Calmodulin-4 OS=Mus musculus OX=10090 GN=Calm4 PE=2 SV=2)

HSP 1 Score: 58.9214 bits (141), Expect = 5.651e-10
Identity = 46/138 (33.33%), Postives = 79/138 (57.25%), Query Frame = 1
Query:  184 EFLQIFYDIDKDKSGAIELNEVEIYLRKHG--FQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNK--HSIKKKQLSDFVQKXXXXXXXXXXYNEFLKF 585
            EF   F   DK+K G I + E+   +++ G     + +K  I   D + DGKI+F+EF+ +++K    HR   E  ++F  LD++ +GYIT+ EL    +ES +K   S+ +++L D ++  D+D DG + Y EF++ 
Sbjct:   12 EFQAAFNRFDKNKDGHISVEELGDVMKQLGKNLPEKDLKALISKLDTDGDGKISFEEFLTAIEKYKKGHR-AGELRAVFNVLDQNGDGYITVDEL----KESLSKLGESLSQEELEDMIRVADVDQDGKVKYEEFVRL 144          
BLAST of Calcium dependent protein kinase 5 vs. UniProt/SwissProt
Match: sp|Q24214|CANB2_DROME (Calcineurin subunit B type 2 OS=Drosophila melanogaster OX=7227 GN=CanB2 PE=1 SV=2)

HSP 1 Score: 52.373 bits (124), Expect = 1.956e-7
Identity = 34/112 (30.36%), Postives = 60/112 (53.57%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SGA+ ++E          Q+  ++  ID FD + +G++ FKEFI  + +  +K    ++    F+  D D++GYI+ GELF +++     +++K  QL   V K
Sbjct:   27 FRKLDLDNSGALSVDEFMSLPELQ--QNPLVQRVIDIFDADGNGEVDFKEFIQGVSQFSVKGDKLSKLRFAFRIYDMDNDGYISNGELFQVLKMM-VGNNLKDTQLQQIVDK 135          
BLAST of Calcium dependent protein kinase 5 vs. UniProt/SwissProt
Match: sp|P48451|CANB1_DROME (Calcineurin subunit B type 1 OS=Drosophila melanogaster OX=7227 GN=CanB PE=2 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 3.154e-7
Identity = 33/112 (29.46%), Postives = 60/112 (53.57%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SGA+ ++E          Q+  ++  ID FD + +G++ FKEFI  + +  ++    ++    F+  D D++GYI+ GELF +++     +++K  QL   V K
Sbjct:   27 FRKLDLDNSGALSIDEFMSLPELQ--QNPLVQRVIDIFDADGNGEVDFKEFIQGVSQFSVRGDKLSKLRFAFRIYDMDNDGYISNGELFQVLKMM-VGNNLKDTQLQQIVDK 135          
BLAST of Calcium dependent protein kinase 5 vs. TrEMBL
Match: A0A504Y705 (Putative calcium-binding protein OS=Fasciola gigantica OX=46835 GN=FGIG_03582 PE=4 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 8.458e-30
Identity = 65/131 (49.62%), Postives = 91/131 (69.47%), Query Frame = 1
Query:  196 IFYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQKXXXXXXXXXXYNEFLKFI 588
            +FY ID+DKSG I+  E+  YLRK+ F   FI  F+  FD ++DGKITF+E+  SL K+P   + YT W  L  ++D+D NGYIT+ EL +++QES N+++I  KQL  FV++ D D DG LNY+EFL +I
Sbjct:    1 MFYLIDRDKSGVIDCEELGQYLRKNNFDDFFITRFLKAFDADRDGKITFEEYRRSLHKIPHVQKTYTTWHQLLCKVDKDKNGYITMDELCDVLQESGNENTINVKQLQSFVREYDRDGDGKLNYHEFLDYI 131          
BLAST of Calcium dependent protein kinase 5 vs. TrEMBL
Match: A0A4E0RVT5 (Putative calcium-binding protein OS=Fasciola hepatica OX=6192 GN=D915_007584 PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 9.481e-29
Identity = 64/131 (48.85%), Postives = 90/131 (68.70%), Query Frame = 1
Query:  196 IFYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQKXXXXXXXXXXYNEFLKFI 588
            +FY ID+DKSG I+  E+  YLR + F   FI  F+  FD ++DGKITF+E+  SL K+P   + YT W  L  ++D+D NGYIT+ EL++++QES N++ I  KQL  FV++ D D DG LNY+EFL +I
Sbjct:    1 MFYLIDRDKSGVIDCEELGQYLRNNNFDDFFITRFLKAFDADRDGKITFEEYRRSLHKIPHVQKTYTTWHQLLCKVDKDKNGYITMDELYDVLQESGNENIINVKQLQSFVREYDRDGDGKLNYHEFLDYI 131          
BLAST of Calcium dependent protein kinase 5 vs. TrEMBL
Match: A0A183APJ2 (Uncharacterized protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS8877 PE=4 SV=1)

HSP 1 Score: 112.079 bits (279), Expect = 1.331e-27
Identity = 61/131 (46.56%), Postives = 88/131 (67.18%), Query Frame = 1
Query:  196 IFYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQKXXXXXXXXXXYNEFLKFI 588
            +FY ID DKSG I+ +E+  YLR + F   FI  F+  FD ++DGKITF+EF  +L K+P   + YT W  +  ++D+D NGYIT+ EL+ ++QE+ + + I  KQL  FV++ D D DG LNY+EFL +I
Sbjct:    1 MFYTIDHDKSGVIDCDELRDYLRSNNFDDFFISRFLRAFDADRDGKITFEEFRRALHKVPHVQKTYTTWHHILHKVDKDKNGYITMDELYELLQENGDSNGISVKQLQSFVREYDRDGDGKLNYHEFLDYI 131          
BLAST of Calcium dependent protein kinase 5 vs. TrEMBL
Match: A0A3Q0KP07 (Putative calcium-binding protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 6.636e-26
Identity = 61/140 (43.57%), Postives = 92/140 (65.71%), Query Frame = 1
Query:  169 METEEEFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQKXXXXXXXXXXYNEFLKFI 588
            M T++  +Q+FYDID DKSGA++ +E+  YL K  F   FI  F+ TFD N+DG ITF+E+   + K+    + Y  ++ + K+LD+D NGYIT  E+++++ ES NK+    K L  F++K D D DG LNY+EFL ++
Sbjct:   23 MTTDDALIQMFYDIDVDKSGAVDCDELREYLIKKEFGECFITRFLRTFDSNRDGMITFEEYQTGIHKISHLRKKYFNYYHILKKLDKDKNGYITTHEIYDVLYESGNKNYFSSKDLQAFIRKYDQDGDGKLNYHEFLDYL 162          
BLAST of Calcium dependent protein kinase 5 vs. TrEMBL
Match: G4VQR1 (Putative calcium-binding protein OS=Schistosoma mansoni OX=6183 GN=Smp_146220 PE=4 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 1.976e-24
Identity = 63/148 (42.57%), Postives = 95/148 (64.19%), Query Frame = 1
Query:  145 KITTRKYLMETEEEFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQKXXXXXXXXXXYNEFLKFI 588
            K+TT   L++     +Q+FYDID DKSGA++ +E+  YL K  F   FI  F+ TFD N+DG ITF+E+   + K+    + Y  ++ + K+LD+D NGYIT  E+++++ ES NK+    K L  F++K D D DG LNY+EFL ++
Sbjct:   22 KMTTDDALIQ-----MQMFYDIDVDKSGAVDCDELREYLIKKEFGECFITRFLRTFDSNRDGMITFEEYQTGIHKISHLRKKYFNYYHILKKLDKDKNGYITTHEIYDVLYESGNKNYFSSKDLQAFIRKYDQDGDGKLNYHEFLDYL 164          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Cavefish
Match: ENSAMXT00000049698.1 (calcineurin subunit B type 1 [Source:NCBI gene;Acc:103042847])

HSP 1 Score: 47.7506 bits (112), Expect = 1.063e-6
Identity = 33/112 (29.46%), Postives = 57/112 (50.89%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SG++ + E          Q+  ++  ID FD + +G++ FKEFI  + +  +K     +    F+  D D +GYI+ GELF +++     +++K  QL   V K
Sbjct:   25 FKKLDLDNSGSLSVEEFMSLPELQ--QNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVG-NNLKDTQLQQIVDK 133          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Cavefish
Match: ENSAMXT00000045317.1 (calcineurin subunit B type 1 [Source:NCBI gene;Acc:103042847])

HSP 1 Score: 47.7506 bits (112), Expect = 1.116e-6
Identity = 33/112 (29.46%), Postives = 57/112 (50.89%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SG++ + E          Q+  ++  ID FD + +G++ FKEFI  + +  +K     +    F+  D D +GYI+ GELF +++     +++K  QL   V K
Sbjct:   25 FKKLDLDNSGSLSVEEFMSLPELQ--QNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVG-NNLKDTQLQQIVDK 133          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Cavefish
Match: ENSAMXT00000038285.1 (calcineurin subunit B type 1 [Source:NCBI gene;Acc:103042847])

HSP 1 Score: 47.7506 bits (112), Expect = 1.134e-6
Identity = 33/112 (29.46%), Postives = 57/112 (50.89%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SG++ + E          Q+  ++  ID FD + +G++ FKEFI  + +  +K     +    F+  D D +GYI+ GELF +++     +++K  QL   V K
Sbjct:   27 FKKLDLDNSGSLSVEEFMSLPELQ--QNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVG-NNLKDTQLQQIVDK 135          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Cavefish
Match: ppp3r1a (calcineurin subunit B type 1 [Source:NCBI gene;Acc:103037384])

HSP 1 Score: 46.595 bits (109), Expect = 2.709e-6
Identity = 32/112 (28.57%), Postives = 57/112 (50.89%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SG++ + E          Q+  ++  ID FD + +G++ F+EFI  + +  +K     +    F+  D D +GYI+ GELF +++     +++K  QL   V K
Sbjct:   27 FKKLDLDNSGSLSVEEFMSLPELQ--QNPLVQRVIDIFDTDGNGEVDFQEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVG-NNLKDTQLQQIVDK 135          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Cavefish
Match: cetn2 (centrin-1 [Source:NCBI gene;Acc:103028142])

HSP 1 Score: 46.595 bits (109), Expect = 3.340e-6
Identity = 31/105 (29.52%), Postives = 53/105 (50.48%), Query Frame = 1
Query:  172 ETEEEFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQS--EFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQE 480
            E ++E  + F   D D SG IE+ E+++ +R  GF+   E IK  I   D +  GKI+F +F+  + +   +  +  E    F+  D+D  G I+   L  + +E
Sbjct:   28 EMKQEIREAFELFDTDGSGHIEVKELKVAMRALGFEPKKEEIKKMIADVDKDGTGKISFNDFLTIMTQKMAEKDSKEEILKAFRLFDDDETGKISFRNLKRVAKE 132          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009304.1 (pep scaffold:Pmarinus_7.0:GL479988:26077:30241:1 gene:ENSPMAG00000008415.1 transcript:ENSPMAT00000009304.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.521 bits (114), Expect = 1.327e-7
Identity = 33/112 (29.46%), Postives = 58/112 (51.79%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SG++ ++E          Q+  ++  ID FD + +G++ FKEFI  + +  +K     +    F+  D D +GYI+ GELF +++     +++K  QL   V K
Sbjct:   27 FRKLDLDNSGSLSVDEFMSLPELQ--QNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKDQKLRFAFRIYDMDKDGYISNGELFQVLKMM-VGNNLKDTQLQQIVDK 135          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004125.1 (pep scaffold:Pmarinus_7.0:GL483005:7747:12994:-1 gene:ENSPMAG00000003768.1 transcript:ENSPMAT00000004125.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 47.7506 bits (112), Expect = 1.929e-7
Identity = 30/112 (26.79%), Postives = 55/112 (49.11%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F+ +D D SGA+  +E       H   +  ++  +D  D + DG++ F EFI  + +  +      +    F+  D D +G+IT GELF +++    ++ ++  QL   V K
Sbjct:   21 FHKLDLDSSGALSADEFLSLPELH--NNPLVRRVVDILDSDGDGEVDFAEFIEGISRFTINGTKEEKLKFAFQIYDVDRDGFITNGELFGVLKTMVGRN-LRDPQLQQIVDK 129          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Sea Lamprey
Match: cetn3 (centrin 3 [Source:ZFIN;Acc:ZDB-GENE-050522-152])

HSP 1 Score: 46.9802 bits (110), Expect = 3.733e-7
Identity = 30/106 (28.30%), Postives = 56/106 (52.83%), Query Frame = 1
Query:  172 ETEEEFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQ---SEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQE 480
            E ++E  + F   D DK GAI+ +E+++ +R  GF+   ++ IK+  D +D    GKI++ +F   + +  L+     E    F+  DE  +G I + +L  + +E
Sbjct:   26 EQKQEIQEAFELFDTDKDGAIDYHELKVAIRALGFEVSKADVIKIMKD-YDREDTGKISYDDFCEVMTERVLERDPLDEVLKAFRLFDEGESGRIGVRDLRRVARE 130          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Sea Lamprey
Match: CETN1 (centrin 1 [Source:HGNC Symbol;Acc:HGNC:1866])

HSP 1 Score: 46.9802 bits (110), Expect = 4.315e-7
Identity = 37/142 (26.06%), Postives = 68/142 (47.89%), Query Frame = 1
Query:   73 SNPVGNEIQGVAVHYIIL*WIYSP---KITTRKYLMETEEEFLQIFYDI-DKDKSGAIELNEVEIYLRKHGFQS--EFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQE 480
            S+PV + +   A  +  L         K   +  L E +++ ++  +D+ D D SG I++ E+++ +R  GF+   E IK  I  FD +  GKI F +F+  + +   +  +  E    F+  D+D  G I+   L  + +E
Sbjct:    2 SDPVPHLLSSQATGFRKLGGAGGAQRKKSGPKPELSEEQKQEIKEAFDLFDADGSGTIDVKELKVAMRALGFEPKKEEIKKMIADFDKDGSGKINFNDFLAMMTQKMSEKDSREEILKAFRLFDDDETGKISFKNLKRVAKE 143          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000781.1 (pep scaffold:Pmarinus_7.0:GL476952:2198:8857:1 gene:ENSPMAG00000000709.1 transcript:ENSPMAT00000000781.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 46.595 bits (109), Expect = 5.233e-7
Identity = 31/112 (27.68%), Postives = 56/112 (50.00%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SG++ + E          Q+  ++  ID FD + +G++ FKEFI  + +  +K     +    +   + D +GYI+ GELF +++     +++K  QL   V K
Sbjct:   27 FRKLDLDGSGSLSVEEFMSLPELQ--QNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRCKYSSCNMDRDGYISNGELFQVLKMMVG-NNLKDTQLQQIVDK 135          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Nematostella
Match: EDO45947 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRG9])

HSP 1 Score: 53.5286 bits (127), Expect = 4.706e-9
Identity = 34/96 (35.42%), Postives = 51/96 (53.12%), Query Frame = 1
Query:  196 IFYDIDKDKSGAIELNEVEIYLRKH---GFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNII 474
            IF  +DKD+SG+I  NE++  L       F  E ++L I  FD + +G I F+EF +  K +       T+W + F+  D DS+G I   EL N +
Sbjct:   20 IFQRVDKDRSGSISSNELQQALSNGTWTPFNPETVRLMIGMFDRDNNGTINFQEFSSLWKYI-------TDWQTTFRNYDRDSSGTIDKNELQNAL 108          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Nematostella
Match: EDO45967 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRJ7])

HSP 1 Score: 49.6766 bits (117), Expect = 1.895e-7
Identity = 38/139 (27.34%), Postives = 68/139 (48.92%), Query Frame = 1
Query:  184 EFLQIFYDIDKDKSGAIELNEVEIYLR--------KHGFQSEFI---KLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNY---------TEWFSLFKRLDEDSNGYITIGEL---FNIIQESDNKHSIKKKQLSDFVQ 531
            EF+++F   D DK+G IE  E+++++         K   ++E     K  ++ +DL+KDG+ +  E  N L   P   R Y          ++  +F R DED +G++   EL   F+ +     K SI  ++L D+++
Sbjct:   12 EFVKVFMKFDSDKNGYIEEKEMDLFIHDLLKELNDKEPTKAEVADKKKEILEKYDLDKDGRFSMDELENILPVEPNLLRTYLSEGKRIGSVDFMKIFTRFDEDKSGFLETVELRGFFHELLSMKGKSSINPRKLDDYIE 150          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Nematostella
Match: EDO45935 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRE8])

HSP 1 Score: 46.9802 bits (110), Expect = 5.354e-7
Identity = 42/149 (28.19%), Postives = 73/149 (48.99%), Query Frame = 1
Query:  154 TRKYLMETEEEFLQIFYDIDKDKSGAIELNEVEIYLRKHG--FQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQE--SDNKHSIKKKQLSDFVQKXXXXXXXXXXYNEFLKFI 588
            T + L E E  F    YD + D  G I   E+   +R+ G     E +K  I   DL+ +GK+ FKEF+  +     +     E  + F R D+D NG+I   E+  +++   S+      K+Q+   ++  D ++DG +++ EF+K +
Sbjct:    6 TPEQLSEIEAAFK--MYDTNGD--GQISAEELGQAMREAGQLVSDEELKDMIRAVDLDGNGKVEFKEFVQMMANQLGQPAPVEEMKAYFDRFDQDGNGFIDSDEMKCLVRAFYSNLTGDALKQQVRAMIEAADTNSDGKISFEEFVKVL 150          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Nematostella
Match: EDO48535 (Predicted protein; Protein phosphatase 3 (Formerly 2B), regulatory subunit B [Source:UniProtKB/TrEMBL;Acc:A7RJ82])

HSP 1 Score: 47.3654 bits (111), Expect = 6.125e-7
Identity = 32/112 (28.57%), Postives = 59/112 (52.68%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SG++ + E          Q+  ++  I  FD + +G++ FKEFI  + +  +K    ++    F+  D D++GYI+ GELF +++     +++K+ QL   V K
Sbjct:   27 FRKLDLDNSGSLSVEEFMSLPELQ--QNPLVQRVITIFDADGNGEVDFKEFIEGVSQFSVKGDKESKLKFAFRIYDMDNDGYISNGELFQVLKMMVG-NNLKETQLQQIVDK 135          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Nematostella
Match: EDO46008 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRG8])

HSP 1 Score: 46.9802 bits (110), Expect = 7.434e-7
Identity = 32/92 (34.78%), Postives = 47/92 (51.09%), Query Frame = 1
Query:  196 IFYDIDKDKSGAIELNEVEIYLRKHG---FQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGEL 462
            IF  ID DK+GAI  +E++  L       F  E ++LF+  FD +  G I F EF +  + +       T+W   F+  D D++G I I EL
Sbjct:   16 IFTRIDADKNGAITGDELQKALSNGSWAPFNPETVRLFMGMFDRDNSGTIEFNEFYSLWQYV-------TDWQKAFRSYDTDNSGTIDIDEL 100          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Medaka
Match: ENSORLT00000002911.2 (protein phosphatase 3 regulatory subunit B, alpha [Source:NCBI gene;Acc:101161991])

HSP 1 Score: 47.7506 bits (112), Expect = 1.127e-6
Identity = 33/112 (29.46%), Postives = 57/112 (50.89%), Query Frame = 1
Query:  199 FYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQK 534
            F  +D D SG++ + E          Q+  ++  ID FD + +G++ FKEFI  + +  +K     +    F+  D D +GYI+ GELF +++     +++K  QL   V K
Sbjct:   27 FKKLDLDNSGSLSVEEFMSLPELQ--QNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVG-NNLKDTQLQQIVDK 135          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Medaka
Match: cetn2 (caltractin [Source:NCBI gene;Acc:101170769])

HSP 1 Score: 45.8246 bits (107), Expect = 5.982e-6
Identity = 35/135 (25.93%), Postives = 61/135 (45.19%), Query Frame = 1
Query:   94 IQGVAVHYIIL*WIYSP---KITTRKYLMETE-EEFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQS--EFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQE 480
            +Q        L     P   K T +  L E + +E  + F   D D SG I++ ++++ +R  GF+   E IK  I   D +  GKI+F +F+  + +   +  +  E    F+  D+D  G I+   L  + +E
Sbjct:    1 MQATTAKRPALQGPVPPPRKKTTPKAELTEEQKQEIREAFELFDTDGSGFIDVKDLKVAMRALGFEPKKEEIKKMIGEVDKDATGKISFADFLTVMTQKMAEKDSKEEILKAFRLFDDDETGKISFRNLKRVAKE 135          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Medaka
Match: cetn2 (caltractin [Source:NCBI gene;Acc:101170769])

HSP 1 Score: 45.8246 bits (107), Expect = 5.982e-6
Identity = 35/135 (25.93%), Postives = 61/135 (45.19%), Query Frame = 1
Query:   94 IQGVAVHYIIL*WIYSP---KITTRKYLMETE-EEFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQS--EFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQE 480
            +Q        L     P   K T +  L E + +E  + F   D D SG I++ ++++ +R  GF+   E IK  I   D +  GKI+F +F+  + +   +  +  E    F+  D+D  G I+   L  + +E
Sbjct:    1 MQATTAKRPALQGPVPPPRKKTTPKAELTEEQKQEIREAFELFDTDGSGFIDVKDLKVAMRALGFEPKKEEIKKMIGEVDKDATGKISFADFLTVMTQKMAEKDSKEEILKAFRLFDDDETGKISFRNLKRVAKE 135          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Medaka
Match: calm2a (calmodulin 2 [Source:NCBI gene;Acc:100049420])

HSP 1 Score: 45.0542 bits (105), Expect = 6.822e-6
Identity = 38/137 (27.74%), Postives = 68/137 (49.64%), Query Frame = 1
Query:  184 EFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKL--FIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQKXXXXXXXXXXYNEFLKFI 588
            EF + F   DKD  G I   E+   +R  G      +L   I+  D + +G I F EF+  + +      +  E    F+  D+D NGYI+  EL +++     K  +  +++ + +++ D+D DG +NY EF++ +
Sbjct:   12 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEK--LTDEEVDEMIREADIDGDGQVNYEEFVQMM 146          
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Medaka
Match: ENSORLT00000008599.2 (calmodulin 3 [Source:NCBI gene;Acc:100049421])

HSP 1 Score: 45.0542 bits (105), Expect = 6.822e-6
Identity = 38/137 (27.74%), Postives = 68/137 (49.64%), Query Frame = 1
Query:  184 EFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKL--FIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQKXXXXXXXXXXYNEFLKFI 588
            EF + F   DKD  G I   E+   +R  G      +L   I+  D + +G I F EF+  + +      +  E    F+  D+D NGYI+  EL +++     K  +  +++ + +++ D+D DG +NY EF++ +
Sbjct:   12 EFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEK--LTDEEVDEMIREADIDGDGQVNYEEFVQMM 146          
BLAST of Calcium dependent protein kinase 5 vs. Planmine SMEST
Match: SMESG000057759.1 (SMESG000057759.1)

HSP 1 Score: 258.84 bits (660), Expect = 4.534e-89
Identity = 143/143 (100.00%), Postives = 143/143 (100.00%), Query Frame = 1
Query:  169 METEEEFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQKXXXXXXXXXXYNEFLKFIGTD 597
            METEEEFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQKNDLDNDGLLNYNEFLKFIGTD
Sbjct:    1 METEEEFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQKNDLDNDGLLNYNEFLKFIGTD 143          
BLAST of Calcium dependent protein kinase 5 vs. Planmine SMEST
Match: SMESG000066843.1 (SMESG000066843.1)

HSP 1 Score: 76.6406 bits (187), Expect = 8.620e-18
Identity = 50/140 (35.71%), Postives = 79/140 (56.43%), Query Frame = 1
Query:  169 METEEEFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQKXXXXXXXXXXYNEFLKFI 588
            M T E+ L+ F  +D D +  +   EV  +L K+GF +E+IK F++TFD NKDGKI+  EF   +       R    W ++FK  D D +G ++  EL  +++     ++  K+ L  F++ +D D DG LNY EF +F+
Sbjct:    1 MATNEQLLEQFAKMDTDHNNELSTEEVTCFLSKNGFAAEYIKKFMETFDANKDGKISKSEFTKRICNQTPHERKIEMWEAVFKSFDTDCSGKLSAKELQAVLK----GNNYDKETLEKFIKSHDKDGDGELNYKEFFEFL 136          
BLAST of Calcium dependent protein kinase 5 vs. Planmine SMEST
Match: SMESG000000553.1 (SMESG000000553.1)

HSP 1 Score: 69.707 bits (169), Expect = 4.144e-15
Identity = 47/140 (33.57%), Postives = 77/140 (55.00%), Query Frame = 1
Query:  169 METEEEFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNIIQESDNKHSIKKKQLSDFVQKXXXXXXXXXXYNEFLKFI 588
            M + E+ +  F ++D++    + + E+   L K GF   FIK F+  FD N+DGKIT  E+   L   P   R Y  W  +F   D D +G ++  E+  +I +S+N     KK L ++++  D +NDG ++Y+EFL F+
Sbjct:    1 MVSNEQLIAKFKEMDRNGDNELSVEEISSSLEKSGFDKTFIKNFLKGFDANEDGKITLNEYKAKLNLYPENERRYNMWRDVFNSFDRDGSGKLSSEEV-GLILKSNN---FDKKTLQNWLRNRDSNNDGEMDYDEFLDFL 136          
BLAST of Calcium dependent protein kinase 5 vs. Planmine SMEST
Match: SMESG000078154.1 (SMESG000078154.1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.724e-11
Identity = 27/67 (40.30%), Postives = 44/67 (65.67%), Query Frame = 1
Query:  184 EFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKH 384
            +FL+ F +ID D SG I   E+  Y+R+H F++EF+  ++  F+ NKDG+IT + +  SL  +P +H
Sbjct:  243 DFLKTFEEIDGDFSGEISYEEISTYMRRHNFEAEFVDKWLTLFEPNKDGRITIESYCGSLGLIPREH 309          
BLAST of Calcium dependent protein kinase 5 vs. Planmine SMEST
Match: SMESG000016862.1 (SMESG000016862.1)

HSP 1 Score: 50.8322 bits (120), Expect = 2.341e-8
Identity = 32/103 (31.07%), Postives = 55/103 (53.40%), Query Frame = 1
Query:  169 METEEEFLQIFYDIDKDKSGAIELNEVEIYLRKHG-FQSEFIKLFIDTFDLNKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFNII 474
            M   E  ++ F ++D D++G +  NEV+  L++ G F  E ++  I  FD+N DGKIT  E+   L  +    + +  W ++F   D D +G + + E+  II
Sbjct:    1 MSLNERLIEKFTEMDLDQNGELTPNEVKYILQQDGKFSKESVEEIIKEFDINSDGKITLDEYKEKLNIVDPVGKRFEIWKNVFDSFDLDHSGGLELEEIQKII 103          
The following BLAST results are available for this feature:
BLAST of Calcium dependent protein kinase 5 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PPP3R21.711e-631.91protein phosphatase 3 regulatory subunit B, beta [... [more]
PPP3R11.755e-629.46protein phosphatase 3 regulatory subunit B, alpha ... [more]
PPP3R21.794e-631.91protein phosphatase 3 regulatory subunit B, beta [... [more]
PPP3R12.103e-629.46protein phosphatase 3 regulatory subunit B, alpha ... [more]
PPP3R12.256e-629.46protein phosphatase 3 regulatory subunit B, alpha ... [more]
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BLAST of Calcium dependent protein kinase 5 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
M04F3.43.110e-934.34pep chromosome:WBcel235:I:4771348:4773070:-1 gene:... [more]
M04F3.43.110e-934.34pep chromosome:WBcel235:I:4771348:4773673:-1 gene:... [more]
M04F3.43.110e-934.34pep chromosome:WBcel235:I:4771600:4773026:-1 gene:... [more]
F43C9.23.681e-829.27pep chromosome:WBcel235:X:4786118:4790323:1 gene:W... [more]
F43C9.23.744e-830.48pep chromosome:WBcel235:X:4783802:4790318:1 gene:W... [more]
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BLAST of Calcium dependent protein kinase 5 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CanB21.951e-830.36gene:FBgn0015614 transcript:FBtr0309866[more]
CanB21.951e-830.36gene:FBgn0015614 transcript:FBtr0088930[more]
CanB3.146e-829.46gene:FBgn0010014 transcript:FBtr0070764[more]
CanB3.146e-829.46gene:FBgn0010014 transcript:FBtr0308348[more]
azot4.382e-731.96gene:FBgn0033238 transcript:FBtr0088853[more]
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BLAST of Calcium dependent protein kinase 5 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ppp3r1a1.067e-629.46protein phosphatase 3, regulatory subunit B, alpha... [more]
ppp3r1b1.338e-629.46protein phosphatase 3 (formerly 2B), regulatory s1... [more]
cetn23.306e-629.52centrin, EF-hand protein, 2 [Source:ZFIN;Acc:ZDB-G... [more]
ncaldb3.973e-635.24neurocalcin delta b [Source:NCBI gene;Acc:445319][more]
ncaldb3.973e-635.24neurocalcin delta b [Source:NCBI gene;Acc:445319][more]
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BLAST of Calcium dependent protein kinase 5 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
hspe11.906e-629.46heat shock protein family E (Hsp10) member 1 [Sour... [more]
cetn14.291e-626.27centrin 1 [Source:NCBI gene;Acc:549903][more]
cetn15.400e-626.27centrin 1 [Source:NCBI gene;Acc:549903][more]
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BLAST of Calcium dependent protein kinase 5 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Calm48.079e-1133.33calmodulin 4 [Source:MGI Symbol;Acc:MGI:1931464][more]
Cetn11.050e-627.27centrin 1 [Source:MGI Symbol;Acc:MGI:1347086][more]
Cetn11.050e-627.27centrin 1 [Source:MGI Symbol;Acc:MGI:1347086][more]
Cetn11.050e-627.27centrin 1 [Source:MGI Symbol;Acc:MGI:1347086][more]
Ppp3r11.443e-629.46protein phosphatase 3, regulatory subunit B, alpha... [more]
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BLAST of Calcium dependent protein kinase 5 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q07167|SM16_SCHMA3.307e-1731.4316 kDa calcium-binding protein OS=Schistosoma mans... [more]
sp|P05933|CALM_SCHPO1.164e-1030.46Calmodulin OS=Schizosaccharomyces pombe (strain 97... [more]
sp|Q9JM83|CALM4_MOUSE5.651e-1033.33Calmodulin-4 OS=Mus musculus OX=10090 GN=Calm4 PE=... [more]
sp|Q24214|CANB2_DROME1.956e-730.36Calcineurin subunit B type 2 OS=Drosophila melanog... [more]
sp|P48451|CANB1_DROME3.154e-729.46Calcineurin subunit B type 1 OS=Drosophila melanog... [more]
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BLAST of Calcium dependent protein kinase 5 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A504Y7058.458e-3049.62Putative calcium-binding protein OS=Fasciola gigan... [more]
A0A4E0RVT59.481e-2948.85Putative calcium-binding protein OS=Fasciola hepat... [more]
A0A183APJ21.331e-2746.56Uncharacterized protein OS=Echinostoma caproni OX=... [more]
A0A3Q0KP076.636e-2643.57Putative calcium-binding protein OS=Schistosoma ma... [more]
G4VQR11.976e-2442.57Putative calcium-binding protein OS=Schistosoma ma... [more]
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BLAST of Calcium dependent protein kinase 5 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000049698.11.063e-629.46calcineurin subunit B type 1 [Source:NCBI gene;Acc... [more]
ENSAMXT00000045317.11.116e-629.46calcineurin subunit B type 1 [Source:NCBI gene;Acc... [more]
ENSAMXT00000038285.11.134e-629.46calcineurin subunit B type 1 [Source:NCBI gene;Acc... [more]
ppp3r1a2.709e-628.57calcineurin subunit B type 1 [Source:NCBI gene;Acc... [more]
cetn23.340e-629.52centrin-1 [Source:NCBI gene;Acc:103028142][more]
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BLAST of Calcium dependent protein kinase 5 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000009304.11.327e-729.46pep scaffold:Pmarinus_7.0:GL479988:26077:30241:1 g... [more]
ENSPMAT00000004125.11.929e-726.79pep scaffold:Pmarinus_7.0:GL483005:7747:12994:-1 g... [more]
cetn33.733e-728.30centrin 3 [Source:ZFIN;Acc:ZDB-GENE-050522-152][more]
CETN14.315e-726.06centrin 1 [Source:HGNC Symbol;Acc:HGNC:1866][more]
ENSPMAT00000000781.15.233e-727.68pep scaffold:Pmarinus_7.0:GL476952:2198:8857:1 gen... [more]
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BLAST of Calcium dependent protein kinase 5 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium dependent protein kinase 5 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO459474.706e-935.42Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO459671.895e-727.34Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO459355.354e-728.19Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO485356.125e-728.57Predicted protein; Protein phosphatase 3 (Formerly... [more]
EDO460087.434e-734.78Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Calcium dependent protein kinase 5 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000002911.21.127e-629.46protein phosphatase 3 regulatory subunit B, alpha ... [more]
cetn25.982e-625.93caltractin [Source:NCBI gene;Acc:101170769][more]
cetn25.982e-625.93caltractin [Source:NCBI gene;Acc:101170769][more]
calm2a6.822e-627.74calmodulin 2 [Source:NCBI gene;Acc:100049420][more]
ENSORLT00000008599.26.822e-627.74calmodulin 3 [Source:NCBI gene;Acc:100049421][more]
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BLAST of Calcium dependent protein kinase 5 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000057759.14.534e-89100.00SMESG000057759.1[more]
SMESG000066843.18.620e-1835.71SMESG000066843.1[more]
SMESG000000553.14.144e-1533.57SMESG000000553.1[more]
SMESG000078154.11.724e-1140.30SMESG000078154.1[more]
SMESG000016862.12.341e-831.07SMESG000016862.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027240 ID=SMED30027240|Name=Calcium dependent protein kinase 5|organism=Schmidtea mediterranea sexual|type=transcript|length=637bp
ATGAAAAAATGAACATCAATTAAAACACAAATCCCCACCCAATGTGAACA
ACATTTCAATGAATAACAACCTAGCAACCCTGTAGGTAATGAGATACAGG
GCGTGGCTGTTCATTATATTATCTTATAATGGATATATTCTCCTAAAATA
ACCACTCGAAAATATTTAATGGAAACTGAAGAAGAATTTTTACAGATTTT
TTATGACATTGATAAAGACAAATCCGGCGCCATCGAATTGAATGAGGTTG
AAATTTATCTGAGAAAACATGGCTTCCAAAGCGAATTTATTAAACTTTTC
ATAGATACATTCGATTTAAACAAAGATGGGAAAATAACTTTTAAGGAATT
TATCAATTCACTAAAAAAGATGCCTCTAAAACACAGAAATTATACCGAAT
GGTTCAGCCTGTTCAAACGACTGGATGAAGACTCAAACGGATACATAACC
ATTGGGGAGTTATTTAACATTATACAAGAATCTGACAATAAACATTCCAT
TAAGAAAAAACAATTAAGCGATTTTGTTCAAAAGAACGATTTGGACAACG
ACGGATTATTAAATTACAACGAATTTCTCAAATTCATTGGAACCGACTGA
ACTCTGAAATACTTCAAGCACCAAAATAGAAAAAGAC
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protein sequence of SMED30027240-orf-1

>SMED30027240-orf-1 ID=SMED30027240-orf-1|Name=SMED30027240-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=144bp
METEEEFLQIFYDIDKDKSGAIELNEVEIYLRKHGFQSEFIKLFIDTFDL
NKDGKITFKEFINSLKKMPLKHRNYTEWFSLFKRLDEDSNGYITIGELFN
IIQESDNKHSIKKKQLSDFVQKNDLDNDGLLNYNEFLKFIGTD*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0004672protein kinase activity
GO:0005524ATP binding
GO:0016301kinase activity
GO:0005509calcium ion binding
Vocabulary: biological process
TermDefinition
GO:0006468protein phosphorylation
GO:0016310phosphorylation
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR002048EF_hand_dom
IPR011992EF-hand-dom_pair
IPR018247EF_Hand_1_Ca_BS
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 115..143
e-value: 5.9
score: 11.5
coord: 6..34
e-value: 1.1
score: 17.5
coord: 40..68
e-value: 0.045
score: 22.9
coord: 77..105
e-value: 0.3
score: 20.2
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 79..140
e-value: 6.6E-10
score: 39.3
coord: 5..63
e-value: 4.7E-9
score: 36.6
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 73..108
score: 10.748
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 36..71
score: 12.84
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 111..143
score: 9.492
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 2..35
score: 7.846
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 1..72
e-value: 1.3E-14
score: 55.7
NoneNo IPR availableGENE3DG3DSA:1.10.238.10coord: 73..141
e-value: 4.6E-12
score: 47.8
NoneNo IPR availablePANTHERPTHR23050CALCIUM BINDING PROTEINcoord: 2..139
NoneNo IPR availablePANTHERPTHR23050:SF224CALMODULIN-LIKE PROTEIN 5coord: 2..139
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 49..61
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 86..98
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 124..136
IPR011992EF-hand domain pairSUPERFAMILYSSF47473EF-handcoord: 4..140