SMED30027223
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30027223 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Alignments
SMED30027223 aligns in the following genomic locations:
Homology
BLAST of SMED30027223 vs. Ensembl Fly
Match: CG8560 (gene:FBgn0035781 transcript:FBtr0332725) HSP 1 Score: 56.6102 bits (135), Expect = 2.626e-10 Identity = 37/115 (32.17%), Postives = 62/115 (53.91%), Query Frame = 2 Query: 17 LFKALILTGLLSVCIAMKSYEGYKVIELNLKDEREVKLILRLTEEYLADIWKGP-SFHVSTHIMLSPSTFKPFTDKLTSSGIKYKIIIENVQETLE------*NFMKQNLRSSSK 340 LF ++L L SV +A+++Y+GYK+ ++N ++ E +L+LRL+ D + P S S+ +M+ P + F L G+ Y +I EN E+L N NLRS+ + Sbjct: 4 LFAVVLL--LASVALAVENYDGYKIYDINARNAFEKQLLLRLSGNEAYDFFDLPRSLDASSRVMVKPEDQEGFEHLLEKYGVNYSVINENFGESLRQERDENQNQRLMNLRSAER 116
BLAST of SMED30027223 vs. Ensembl Fly
Match: CG8560 (gene:FBgn0035781 transcript:FBtr0076868) HSP 1 Score: 56.6102 bits (135), Expect = 2.626e-10 Identity = 37/115 (32.17%), Postives = 62/115 (53.91%), Query Frame = 2 Query: 17 LFKALILTGLLSVCIAMKSYEGYKVIELNLKDEREVKLILRLTEEYLADIWKGP-SFHVSTHIMLSPSTFKPFTDKLTSSGIKYKIIIENVQETLE------*NFMKQNLRSSSK 340 LF ++L L SV +A+++Y+GYK+ ++N ++ E +L+LRL+ D + P S S+ +M+ P + F L G+ Y +I EN E+L N NLRS+ + Sbjct: 4 LFAVVLL--LASVALAVENYDGYKIYDINARNAFEKQLLLRLSGNEAYDFFDLPRSLDASSRVMVKPEDQEGFEHLLEKYGVNYSVINENFGESLRQERDENQNQRLMNLRSAER 116
BLAST of SMED30027223 vs. Ensembl Zebrafish
Match: cpa5 (carboxypeptidase A5 [Source:ZFIN;Acc:ZDB-GENE-020514-1]) HSP 1 Score: 45.4394 bits (106), Expect = 3.145e-6 Identity = 33/103 (32.04%), Postives = 49/103 (47.57%), Query Frame = 2 Query: 23 KALILTGLLSVCIAMKSYEGYKVIELNLKDEREVKLILRLTE--EYLA-DIWKGP-------SFHVSTHIMLSPSTFKPFTDKLTSSGIKYKIIIENVQETLE 301 + L+L L K++EG +V + KDE ++ L+ LTE E+L +W P HV H + + F L + I Y I+IENVQE L+ Sbjct: 3 RLLVLIALFVAVFGKKTFEGDQVFRITPKDEAQIDLLKELTEEGEHLGLSVWMEPILESLPLDLHVPFHSLQAVRAF------LAYNQIPYHIMIENVQELLD 99
BLAST of SMED30027223 vs. Ensembl Zebrafish
Match: cpa1 (carboxypeptidase A1 (pancreatic) [Source:ZFIN;Acc:ZDB-GENE-050522-438]) HSP 1 Score: 44.2838 bits (103), Expect = 7.362e-6 Identity = 34/120 (28.33%), Postives = 53/120 (44.17%), Query Frame = 2 Query: 29 LILTGLLSVCIAMKSYEGYKVIELNLKDEREVKLILRLTEEYL--ADIWKGPS-------FHVSTHIMLSPSTFKPFTDKLTSSGIKYKIIIENVQETL-E*NFMKQNLRSSSKKEDSFI 358 L+LT L ++EG +V+ +N K+ ++ L+ LTE L D W P+ HV H + + F L + I Y I+IENVQ+ + E R + D F+ Sbjct: 5 LVLTALFVAVFGKVTFEGDQVLRINAKNAEQISLLKELTEADLLGLDFWMTPARESLPVDLHVPFHSLQAVRAF------LAYNQIPYHIMIENVQDLVDEEQLQMLKYRGFPRSTDDFV 118
BLAST of SMED30027223 vs. TrEMBL
Match: B4HV67 (GM13761 OS=Drosophila sechellia OX=7238 GN=Dsec\GM13761 PE=4 SV=1) HSP 1 Score: 59.3066 bits (142), Expect = 1.165e-7 Identity = 39/115 (33.91%), Postives = 61/115 (53.04%), Query Frame = 2 Query: 17 LFKALILTGLLSVCIAMKSYEGYKVIELNLKDEREVKLILRLTEEYLADIWKGP-SFHVSTHIMLSPSTFKPFTDKLTSSGIKYKIIIENVQETLE------*NFMKQNLRSSSK 340 LF A++L L SV +A+ YEGYK+ ++N ++ E +L+LRL+ D + P S S+ +M+ P + F L G+ Y +I EN E+L N NLRS+ + Sbjct: 4 LFAAVLL--LASVALAVDDYEGYKIYDINARNSFEKQLLLRLSGNEAYDFFDLPRSLDASSRVMVKPEDQEGFEHLLEKYGVNYSVINENFGESLRQERDENQNQRLMNLRSAER 116
BLAST of SMED30027223 vs. TrEMBL
Match: A0A0L0CSG6 (Peptidase_M14 domain-containing protein OS=Lucilia cuprina OX=7375 GN=FF38_05970 PE=4 SV=1) HSP 1 Score: 57.7658 bits (138), Expect = 4.724e-7 Identity = 34/112 (30.36%), Postives = 61/112 (54.46%), Query Frame = 2 Query: 5 NQNMLFKALILTGLLSVCIAMKSYEGYKVIELNLKDEREVKLILRLTEEYLADIWKGPSFHVSTHIMLSPSTFKPFTDKLTSSGIKYKIIIENVQETLE*NFMKQNLRSSSK 340 N ++ L+ + VCI SY+GYK+ EL K+E+++ L+ L ++ + F S +++ P++F+ F + L +KYKI+ E+V +TL+ NF L + K Sbjct: 1238 NFRLMVVGLLWANIYFVCINANSYDGYKLYELLPKEEKDLNLLKNLNQQE-----ERYDFLNSQTVIVEPNSFREFENTLNKYNLKYKIVDEDVGKTLKRNFEVNGLLKTLK 1344
BLAST of SMED30027223 vs. TrEMBL
Match: Q961J8 (GH20109p OS=Drosophila melanogaster OX=7227 GN=Dmel\CG8560 PE=1 SV=1) HSP 1 Score: 56.6102 bits (135), Expect = 7.803e-7 Identity = 37/115 (32.17%), Postives = 62/115 (53.91%), Query Frame = 2 Query: 17 LFKALILTGLLSVCIAMKSYEGYKVIELNLKDEREVKLILRLTEEYLADIWKGP-SFHVSTHIMLSPSTFKPFTDKLTSSGIKYKIIIENVQETLE------*NFMKQNLRSSSK 340 LF ++L L SV +A+++Y+GYK+ ++N ++ E +L+LRL+ D + P S S+ +M+ P + F L G+ Y +I EN E+L N NLRS+ + Sbjct: 4 LFAVVLL--LASVALAVENYDGYKIYDINARNAFEKQLLLRLSGNEAYDFFDLPRSLDASSRVMVKPEDQEGFEHLLEKYGVNYSVINENFGESLRQERDENQNQRLMNLRSAER 116
BLAST of SMED30027223 vs. TrEMBL
Match: A0A232EFF3 (Uncharacterized protein OS=Trichomalopsis sarcophagae OX=543379 GN=TSAR_013263 PE=4 SV=1) HSP 1 Score: 55.0694 bits (131), Expect = 1.173e-6 Identity = 31/90 (34.44%), Postives = 49/90 (54.44%), Query Frame = 2 Query: 44 LLSVCIAMK---SYEGYKVIELNLKDEREVKLILRLTEEYLA-DIWKGPSFHVSTHIMLSPSTFKPFTDKLTSSGIKYKIIIENVQETLE 301 L++ IA SYE YKV ++ +K + E+K + L E W GPS + T IM++ FT+ + SGIKY+ ++NV+ +E Sbjct: 9 LMTATIAAAEKVSYENYKVFQMVIKKDDELKFLKDLVESRDDLSFWSGPSGSIPTDIMVAAKALDYFTEHINDSGIKYEKFVDNVEALIE 98
BLAST of SMED30027223 vs. TrEMBL
Match: B4QKE7 (GD13057 OS=Drosophila simulans OX=7240 GN=Dsim\GD13057 PE=4 SV=1) HSP 1 Score: 56.225 bits (134), Expect = 1.292e-6 Identity = 38/115 (33.04%), Postives = 60/115 (52.17%), Query Frame = 2 Query: 17 LFKALILTGLLSVCIAMKSYEGYKVIELNLKDEREVKLILRLTEEYLADIWKGP-SFHVSTHIMLSPSTFKPFTDKLTSSGIKYKIIIENVQETLE------*NFMKQNLRSSSK 340 LF A++L L SV +A+ YEGYK+ ++N ++ E +L+LRL+ D + P S + +M+ P + F L G+ Y +I EN E+L N NLRS+ + Sbjct: 4 LFAAVLL--LASVALAVDDYEGYKIYDINARNAFEKQLLLRLSGNEAYDFFDLPRSLDAPSRVMVKPEDQEGFEHLLEKYGVNYLVINENFGESLRQERDENQNQRLMNLRSAER 116
BLAST of SMED30027223 vs. Ensembl Cavefish
Match: ENSAMXT00000011968.2 (carboxypeptidase A1-like [Source:NCBI gene;Acc:111195717]) HSP 1 Score: 44.669 bits (104), Expect = 5.504e-6 Identity = 31/95 (32.63%), Postives = 54/95 (56.84%), Query Frame = 2 Query: 29 LILTGLLSVCIAMKSYEGYKVIELNLKDEREVKLILRLTE-EYLA-DIWKGPSFHVSTHIMLS-PST-FKPFTDKLTSSGIKYKIIIENVQETLE 301 L+L L+ KS+EG++V+ + +D+ ++ L+ LT+ YL D W+ P H S + +S PST + L ++ I Y I+I N+Q+ L+ Sbjct: 5 LVLASLVVAVFGRKSFEGHQVLRITAEDDLQINLLKNLTQLAYLKLDFWREP-IHESLPVDVSVPSTSLQTLKSFLKNNNILYTIMIHNIQDLLD 98
BLAST of SMED30027223 vs. Planmine SMEST
Match: SMESG000066355.1 (SMESG000066355.1) HSP 1 Score: 194.897 bits (494), Expect = 5.398e-66 Identity = 96/96 (100.00%), Postives = 96/96 (100.00%), Query Frame = 2 Query: 14 MLFKALILTGLLSVCIAMKSYEGYKVIELNLKDEREVKLILRLTEEYLADIWKGPSFHVSTHIMLSPSTFKPFTDKLTSSGIKYKIIIENVQETLE 301 MLFKALILTGLLSVCIAMKSYEGYKVIELNLKDEREVKLILRLTEEYLADIWKGPSFHVSTHIMLSPSTFKPFTDKLTSSGIKYKIIIENVQETLE Sbjct: 1 MLFKALILTGLLSVCIAMKSYEGYKVIELNLKDEREVKLILRLTEEYLADIWKGPSFHVSTHIMLSPSTFKPFTDKLTSSGIKYKIIIENVQETLE 96
BLAST of SMED30027223 vs. Planmine SMEST
Match: SMESG000066357.1 (SMESG000066357.1) HSP 1 Score: 169.088 bits (427), Expect = 8.394e-56 Identity = 86/97 (88.66%), Postives = 90/97 (92.78%), Query Frame = 2 Query: 14 MLFKALILTGLLSVCIAMKSYEGYKVIELNLKDEREVKLILRLTEEYLADIW-KGPSFHVSTHIMLSPSTFKPFTDKLTSSGIKYKIIIENVQETLE 301 MLFKALILT LLSVCIAMKSYEGYKVIELNLKDEREVKLI RL +EYLADIW +GPSF ST+IMLSPSTFKPFTDKLTSSGIKYKI IENVQ+TLE Sbjct: 1 MLFKALILTALLSVCIAMKSYEGYKVIELNLKDEREVKLIHRLIKEYLADIWIQGPSFRFSTYIMLSPSTFKPFTDKLTSSGIKYKIKIENVQKTLE 97
BLAST of SMED30027223 vs. Planmine SMEST
Match: SMESG000066359.1 (SMESG000066359.1) HSP 1 Score: 141.354 bits (355), Expect = 1.919e-44 Identity = 69/78 (88.46%), Postives = 73/78 (93.59%), Query Frame = 2 Query: 68 KSYEGYKVIELNLKDEREVKLILRLTEEYLADIWKGPSFHVSTHIMLSPSTFKPFTDKLTSSGIKYKIIIENVQETLE 301 ++Y YKVIELNLKDEREVKLILRLTEEYLADIWKGPS +STHIMLSPSTFKPFTDKLTSSGIKYKI IENVQ+TLE Sbjct: 54 QNYHKYKVIELNLKDEREVKLILRLTEEYLADIWKGPSVRISTHIMLSPSTFKPFTDKLTSSGIKYKIKIENVQKTLE 131
BLAST of SMED30027223 vs. Planmine SMEST
Match: SMESG000066311.1 (SMESG000066311.1) HSP 1 Score: 95.5153 bits (236), Expect = 3.903e-24 Identity = 48/99 (48.48%), Postives = 69/99 (69.70%), Query Frame = 2 Query: 5 NQNMLFKALILTGLLSVCIAMKSYEGYKVIELNLKDEREVKLILRLTEEYLADIWKGPSFHVSTHIMLSPSTFKPFTDKLTSSGIKYKIIIENVQETLE 301 N ++F AL LL A++SY GYKVIE++LK + E+K +L+++E+Y ADIWKGPS +TH+MLSP F F + LT I+YKI+I NVQ+ ++ Sbjct: 2 NLRLIFVAL----LLGATQALESYNGYKVIEMSLKTDEEIKHVLQISEKYQADIWKGPSKITTTHVMLSPEYFDGFIESLTKYEIQYKIMINNVQKIID 96
BLAST of SMED30027223 vs. Planmine SMEST
Match: SMESG000066416.1 (SMESG000066416.1) HSP 1 Score: 72.7886 bits (177), Expect = 7.885e-18 Identity = 34/92 (36.96%), Postives = 55/92 (59.78%), Query Frame = 2 Query: 14 MLFKALILTGLLSVCIAMKSYEGYKVIELNLKDEREVKLILRLTEEYLADIWKGPSFHVSTHIMLSPSTFKPFTDKLTSSGIKYKIIIENVQ 289 M K LIL +L+VC+A GYKV+ELNL +++EV+ + L + WKGP++ THIML+ K L + + YK++I++++ Sbjct: 1 MFLKVLILATVLAVCLAEFPLRGYKVVELNLHNQKEVRFVANLAVYLKGNFWKGPTYKGPTHIMLNALVAKKLAKILKYAKVDYKVLIDDLE 92 The following BLAST results are available for this feature:
BLAST of SMED30027223 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30027223 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30027223 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 2
BLAST of SMED30027223 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 2
BLAST of SMED30027223 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30027223 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30027223 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30027223 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30027223 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of SMED30027223 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30027223 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30027223 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30027223 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30027223 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30027223 ID=SMED30027223|Name=SMED30027223|organism=Schmidtea mediterranea sexual|type=transcript|length=364bpback to top protein sequence of SMED30027223-orf-1 >SMED30027223-orf-1 ID=SMED30027223-orf-1|Name=SMED30027223-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=101bp NNQNMLFKALILTGLLSVCIAMKSYEGYKVIELNLKDEREVKLILRLTEEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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