G2/M phase-specific E3 ubiquitin-protein ligase

Overview
NameG2/M phase-specific E3 ubiquitin-protein ligase
Smed IDSMED30027120
Length (bp)1569
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G2/M phase-specific E3 ubiquitin-protein ligase (SMED30027120) t-SNE clustered cells

Violin plots show distribution of expression levels for G2/M phase-specific E3 ubiquitin-protein ligase (SMED30027120) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G2/M phase-specific E3 ubiquitin-protein ligase (SMED30027120) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G2/M phase-specific E3 ubiquitin-protein ligase (SMED30027120) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30027120

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30027120h1SMcG0019183 SmedASXL_016302SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30027120h1SMcG0019183 SmedASXL_016302SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30027120h1SMcG0019183 Contig20335uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027120h1SMcG0019183 Contig20335newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027120h1SMcG0019184 Contig20335uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30027120h1SMcG0019184 Contig20335newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30027120h1SMcG0019183 dd_Smed_v4_6301_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30027120h1SMcG0019183 dd_Smed_v4_6301_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30027120h1SMcG0019183 dd_Smed_v4_6301_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30027120h1SMcG0019183 dd_Smed_v4_6301_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30027120h1SMcG0019183 dd_Smed_v4_6301_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30027120h1SMcG0019183 dd_Smed_v4_6301_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Human
Match: PHF7 (PHD finger protein 7 [Source:HGNC Symbol;Acc:HGNC:18458])

HSP 1 Score: 135.576 bits (340), Expect = 4.395e-35
Identity = 80/232 (34.48%), Postives = 115/232 (49.57%), Query Frame = 3 
Query:  111 DEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPE-CAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYE 803
            D   LGE L + N S+H  CL  S  L Q+       +GF  GF   DI+KE  R SR  CFVCKK+ A I C  + C R++HL CG +   +  F   +  +C +H   Q+++      G++  E C  C + ++    E + S                      CC++A  HR C+ K+   S  H  KCP C N+  +    L+ G+ IPDRDAAWE+EPGAF ++Y+
Sbjct:    2 DPEKLGEFLQKDNISVHYFCLILSSKLPQR---GQSNRGF-HGFLPEDIKKEAARASRKICFVCKKKGAAINCQKDQCLRNFHLPCGQERGCLSQFFGEYKSFCDKHRPTQNIQH-----GHVGEESCILCCEDLSQQSVENIQSP---------------------CCSQAIYHRKCIQKYAHTSAKHFFKCPQCNNRKEFPQEMLRMGIHIPDRDAAWELEPGAFSDLYQ 203
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Human
Match: PHF7 (PHD finger protein 7 [Source:HGNC Symbol;Acc:HGNC:18458])

HSP 1 Score: 135.961 bits (341), Expect = 1.076e-34
Identity = 88/275 (32.00%), Postives = 129/275 (46.91%), Query Frame = 3 
Query:  111 DEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPE-CAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGFQMSPDGNPVLPVTHIQGDKGSH 932
            D   LGE L + N S+H  CL  S  L Q+       +GF  GF   DI+KE  R SR  CFVCKK+ A I C  + C R++HL CG +   +  F   +  +C +H   Q+++      G++  E C  C + ++    E + S                      CC++A  HR C+ K+   S  H  KCP C N+  +    L+ G+ IPDRDAAWE+EPGAF ++Y+     + D  I +       F+   +G   L +    G  G+H
Sbjct:   42 DPEKLGEFLQKDNISVHYFCLILSSKLPQR---GQSNRGF-HGFLPEDIKKEAARASRKICFVCKKKGAAINCQKDQCLRNFHLPCGQERGCLSQFFGEYKSFCDKHRPTQNIQH-----GHVGEESCILCCEDLSQQSVENIQSP---------------------CCSQAIYHRKCIQKYAHTSAKHFFKCPQCNNRKEFPQEMLRMGIHIPDRDAAWELEPGAFSDLYQRYQ--HCDAPICLYEQGRDSFE--DEGRWCLILCATCGSHGTH 282
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Human
Match: G2E3 (G2/M-phase specific E3 ubiquitin protein ligase [Source:HGNC Symbol;Acc:HGNC:20338])

HSP 1 Score: 130.568 bits (327), Expect = 4.496e-33
Identity = 81/265 (30.57%), Postives = 121/265 (45.66%), Query Frame = 3 
Query:  147 NFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISS-DSDTGFQM-SPDGNPVLPVTHIQGDKGSHL 935
            N ++H  CL  S  + Q+     K +  + GF I DI KE+ R S+LKC VCKK  A IGC    CKRSYH  CGLQ   +  FT +F  +C +H   Q +                       T N    S+P      F+  + + +++ + CC  AW HR C+    + +G+   +C  C N  ++    L+ G+ IP++DA+WE+E  A+QE+      L H E   +       G    +PD    +      G  G+HL
Sbjct:   37 NLTVHYYCLLMSSGIWQR----GKEEEGVYGFLIEDIRKEVNRASKLKCCVCKKNGASIGCVAPRCKRSYHFPCGLQRECIFQFTGNFASFCWDHRPVQII-----------------------TSNNYRESLPCTICLEFIEPIPSYNILRSPCCKNAWFHRDCLQVQAINAGVFFFRCTICNNSDIFQKEMLRMGIHIPEKDASWELEENAYQEL------LQHYERCDVRRCRCKEGRDYNAPDSKWEIKRCQCCGSSGTHL 268
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Human
Match: G2E3 (G2/M-phase specific E3 ubiquitin protein ligase [Source:HGNC Symbol;Acc:HGNC:20338])

HSP 1 Score: 133.265 bits (334), Expect = 2.320e-32
Identity = 71/219 (32.42%), Postives = 108/219 (49.32%), Query Frame = 3 
Query:  147 NFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYE 803
            N ++H  CL  S  + Q+     K +  + GF I DI KE+ R S+LKC VCKK  A IGC    CKRSYH  CGLQ   +  FT +F  +C +H   Q +                       T N    S+P      F+  + + +++ + CC  AW HR C+    + +G+   +C  C N  ++    L+ G+ IP++DA+WE+E  A+QE+ +
Sbjct:   67 NLTVHYYCLLMSSGIWQR----GKEEEGVYGFLIEDIRKEVNRASKLKCCVCKKNGASIGCVAPRCKRSYHFPCGLQRECIFQFTGNFASFCWDHRPVQII-----------------------TSNNYRESLPCTICLEFIEPIPSYNILRSPCCKNAWFHRDCLQVQAINAGVFFFRCTICNNSDIFQKEMLRMGIHIPEKDASWELEENAYQELLQ 258
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Human
Match: G2E3 (G2/M-phase specific E3 ubiquitin protein ligase [Source:HGNC Symbol;Acc:HGNC:20338])

HSP 1 Score: 132.88 bits (333), Expect = 2.935e-32
Identity = 71/219 (32.42%), Postives = 108/219 (49.32%), Query Frame = 3 
Query:  147 NFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYE 803
            N ++H  CL  S  + Q+     K +  + GF I DI KE+ R S+LKC VCKK  A IGC    CKRSYH  CGLQ   +  FT +F  +C +H   Q +                       T N    S+P      F+  + + +++ + CC  AW HR C+    + +G+   +C  C N  ++    L+ G+ IP++DA+WE+E  A+QE+ +
Sbjct:   37 NLTVHYYCLLMSSGIWQR----GKEEEGVYGFLIEDIRKEVNRASKLKCCVCKKNGASIGCVAPRCKRSYHFPCGLQRECIFQFTGNFASFCWDHRPVQII-----------------------TSNNYRESLPCTICLEFIEPIPSYNILRSPCCKNAWFHRDCLQVQAINAGVFFFRCTICNNSDIFQKEMLRMGIHIPEKDASWELEENAYQELLQ 228
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Fly
Match: Phf7 (gene:FBgn0031091 transcript:FBtr0070027)

HSP 1 Score: 124.405 bits (311), Expect = 3.385e-30
Identity = 75/250 (30.00%), Postives = 117/250 (46.80%), Query Frame = 3 
Query:  111 DEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDT 860
            DE   G +  E N  +H NCL+ S  L Q+ +    +K  I+ F   DIE E+ R   LKC  C++  A I CC   C+R++H  CG+ + A   F D++  +C +H +    R   ++      EC  C + + A + E  + V               + ++A CC   W HR C+ ++  +SG +  KCP C N  V+   A   G+ + D+DA+WE EP AF   Y           + +S  +DT
Sbjct:   14 DELIFGTVHVEGNMMVHRNCLYLSSNLIQRGE----KKLSIMNFLKEDIEAEVNRCRLLKCCYCRRLGANIWCCKSGCRRTFHTKCGVDNLAQNQFCDTYNSFCHQHVLVPRNRPVFKN----DEECLLCAEDVVA-KGERFSVV---------------TCLYAPCCRNGWFHRRCLQRYANSSG-YFFKCPLCNNTDVFRRVAY-MGIAVLDQDASWETEPDAFAGQYRRDVNCTAALCVAVSGRADT 237
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Fly
Match: Phf7 (gene:FBgn0031091 transcript:FBtr0333547)

HSP 1 Score: 124.405 bits (311), Expect = 3.385e-30
Identity = 75/250 (30.00%), Postives = 117/250 (46.80%), Query Frame = 3 
Query:  111 DEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDT 860
            DE   G +  E N  +H NCL+ S  L Q+ +    +K  I+ F   DIE E+ R   LKC  C++  A I CC   C+R++H  CG+ + A   F D++  +C +H +    R   ++      EC  C + + A + E  + V               + ++A CC   W HR C+ ++  +SG +  KCP C N  V+   A   G+ + D+DA+WE EP AF   Y           + +S  +DT
Sbjct:   14 DELIFGTVHVEGNMMVHRNCLYLSSNLIQRGE----KKLSIMNFLKEDIEAEVNRCRLLKCCYCRRLGANIWCCKSGCRRTFHTKCGVDNLAQNQFCDTYNSFCHQHVLVPRNRPVFKN----DEECLLCAEDVVA-KGERFSVV---------------TCLYAPCCRNGWFHRRCLQRYANSSG-YFFKCPLCNNTDVFRRVAY-MGIAVLDQDASWETEPDAFAGQYRRDVNCTAALCVAVSGRADT 237
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Fly
Match: Phf7 (gene:FBgn0031091 transcript:FBtr0333546)

HSP 1 Score: 124.405 bits (311), Expect = 3.385e-30
Identity = 75/250 (30.00%), Postives = 117/250 (46.80%), Query Frame = 3 
Query:  111 DEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDT 860
            DE   G +  E N  +H NCL+ S  L Q+ +    +K  I+ F   DIE E+ R   LKC  C++  A I CC   C+R++H  CG+ + A   F D++  +C +H +    R   ++      EC  C + + A + E  + V               + ++A CC   W HR C+ ++  +SG +  KCP C N  V+   A   G+ + D+DA+WE EP AF   Y           + +S  +DT
Sbjct:   14 DELIFGTVHVEGNMMVHRNCLYLSSNLIQRGE----KKLSIMNFLKEDIEAEVNRCRLLKCCYCRRLGANIWCCKSGCRRTFHTKCGVDNLAQNQFCDTYNSFCHQHVLVPRNRPVFKN----DEECLLCAEDVVA-KGERFSVV---------------TCLYAPCCRNGWFHRRCLQRYANSSG-YFFKCPLCNNTDVFRRVAY-MGIAVLDQDASWETEPDAFAGQYRRDVNCTAALCVAVSGRADT 237
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Fly
Match: pie (gene:FBgn0005683 transcript:FBtr0333490)

HSP 1 Score: 100.523 bits (249), Expect = 4.321e-22
Identity = 66/240 (27.50%), Postives = 111/240 (46.25%), Query Frame = 3 
Query:   87 CSLCDIS-CDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYE 803
            C +C  S  D+   GE +   N  +H  CL  S  L Q+   S      ILGF + DI +E     + KC+ C K  A + C  + C+  +HL CGL++ A+  F   +  YC +     D ++Q++S       C  C  S+   E                       V++  CC   + H+ CM ++ L SG ++++C  C ++  + D+     +++PDRDA WE +  A++E++E
Sbjct:    9 CLICKYSDTDDLVFGEWMIVRNLQVHYFCLLLSTHLPQRGGDS----SGILGFLLRDIREEAAAAEKRKCWYCNKIGASLQC--DRCRSLFHLKCGLENRAVFEFCGQYKSYCYKCRPMDDYKRQLQSNPPRNATCPICFSSIYKVE--------------------LHCVVYGDCCRLGFAHKKCMRQYALTSG-YYLRCIWCRSER-FRDSIRLQSVFVPDRDATWEKQRNAYRELHE 220
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Fly
Match: pie (gene:FBgn0005683 transcript:FBtr0080046)

HSP 1 Score: 100.523 bits (249), Expect = 4.321e-22
Identity = 66/240 (27.50%), Postives = 111/240 (46.25%), Query Frame = 3 
Query:   87 CSLCDIS-CDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYE 803
            C +C  S  D+   GE +   N  +H  CL  S  L Q+   S      ILGF + DI +E     + KC+ C K  A + C  + C+  +HL CGL++ A+  F   +  YC +     D ++Q++S       C  C  S+   E                       V++  CC   + H+ CM ++ L SG ++++C  C ++  + D+     +++PDRDA WE +  A++E++E
Sbjct:    9 CLICKYSDTDDLVFGEWMIVRNLQVHYFCLLLSTHLPQRGGDS----SGILGFLLRDIREEAAAAEKRKCWYCNKIGASLQC--DRCRSLFHLKCGLENRAVFEFCGQYKSYCYKCRPMDDYKRQLQSNPPRNATCPICFSSIYKVE--------------------LHCVVYGDCCRLGFAHKKCMRQYALTSG-YYLRCIWCRSER-FRDSIRLQSVFVPDRDATWEKQRNAYRELHE 220
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Zebrafish
Match: g2e3 (G2/M-phase specific E3 ubiquitin protein ligase [Source:ZFIN;Acc:ZDB-GENE-030616-400])

HSP 1 Score: 123.635 bits (309), Expect = 1.950e-29
Identity = 69/223 (30.94%), Postives = 112/223 (50.22%), Query Frame = 3 
Query:  135 LTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYE 803
            L + N ++H  CL  S  + Q+ +      GF++G    DI+KEI+R SRL+CF CKK  A +GC  + C++  H+ CGL+   +  FT  F  +CK+HA  Q      +S  ++   C+ CLD +                      + +  V+    C  +W HR C+  +  ++ +   KC  C NK  +    L+ G++IP+RDA+WE+E  AF E+ +
Sbjct:   39 LEQHNLAVHFFCLLMSSGICQRGEEDEDVYGFLVG----DIKKEIRRSSRLRCFHCKKAGASVGCSIKSCRQMVHMPCGLEQEFVFQFTGLFPSFCKKHAPTQS----CDSSHSLPHSCSICLDPIEP--------------------ILSYHVLKCPACHGSWFHRKCVQNYAHSAAMFFFKCTLCNNKEQFQQEMLRMGIYIPERDASWELEENAFVELLQ 233
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Zebrafish
Match: phf11 (PHD finger protein 11 [Source:ZFIN;Acc:ZDB-GENE-081104-417])

HSP 1 Score: 79.7221 bits (195), Expect = 1.312e-16
Identity = 40/124 (32.26%), Postives = 69/124 (55.65%), Query Frame = 3 
Query:   69 NTIKRTCSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGI---FTDSFTFYCKEH 431
            +T +  C+LC  S +    G L ++ + S H+NCL FS  ++ +  P+      + GF + D++KE +R  +L+C+ C K  A +GC  + C RS+H  C ++++A  I    T ++  YCK H
Sbjct:    8 STYRIICALCKTSDETETTGPLSSKEDISAHQNCLLFSSNIYCKNSPTYDD---LFGFDLEDVKKEQRRGQKLRCYFCNKTGATVGCEIKRCPRSFHYPCAIKADARAIEDFSTGTYRVYCKSH 128
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Zebrafish
Match: phf11 (PHD finger protein 11 [Source:ZFIN;Acc:ZDB-GENE-081104-417])

HSP 1 Score: 80.1073 bits (196), Expect = 4.561e-16
Identity = 40/124 (32.26%), Postives = 69/124 (55.65%), Query Frame = 3 
Query:   69 NTIKRTCSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGI---FTDSFTFYCKEH 431
            +T +  C+LC  S +    G L ++ + S H+NCL FS  ++ +  P+      + GF + D++KE +R  +L+C+ C K  A +GC  + C RS+H  C ++++A  I    T ++  YCK H
Sbjct:    8 STYRIICALCKTSDETETTGPLSSKEDISAHQNCLLFSSNIYCKNSPTYDD---LFGFDLEDVKKEQRRGQKLRCYFCNKTGATVGCEIKRCPRSFHYPCAIKADARAIEDFSTGTYRVYCKSH 128
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Zebrafish
Match: phf11 (PHD finger protein 11 [Source:ZFIN;Acc:ZDB-GENE-081104-417])

HSP 1 Score: 80.8777 bits (198), Expect = 8.912e-16
Identity = 40/124 (32.26%), Postives = 69/124 (55.65%), Query Frame = 3 
Query:   69 NTIKRTCSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGI---FTDSFTFYCKEH 431
            +T +  C+LC  S +    G L ++ + S H+NCL FS  ++ +  P+      + GF + D++KE +R  +L+C+ C K  A +GC  + C RS+H  C ++++A  I    T ++  YCK H
Sbjct:    8 STYRIICALCKTSDETETTGPLSSKEDISAHQNCLLFSSNIYCKNSPTYDD---LFGFDLEDVKKEQRRGQKLRCYFCNKTGATVGCEIKRCPRSFHYPCAIKADARAIEDFSTGTYRVYCKSH 128
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Zebrafish
Match: phf6 (PHD finger protein 6 [Source:ZFIN;Acc:ZDB-GENE-030131-5278])

HSP 1 Score: 63.929 bits (154), Expect = 4.643e-11
Identity = 41/121 (33.88%), Postives = 59/121 (48.76%), Query Frame = 3 
Query:   81 RTCSLCDISCDEPYLGELLTESN-FSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDS---FTFYCKEH 431
            R C+ C  + D+     L+ ES+  + H  C+ FS  L      S      I GF I D++KEIKR ++L C  C +  A IGC  + C+R+YH  C L   A      S   +  YC++H
Sbjct:   13 RKCAFCRTNRDKECGQLLVAESHRVAAHHKCMLFSSALVTSHSDSEN----IGGFSIEDVKKEIKRGNKLMCTSCHRPGATIGCDVKTCRRTYHYYCALWDKAQTKENPSQGIYLVYCRKH 129
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Xenopus
Match: G2E3 (G2/M-phase specific E3 ubiquitin protein ligase [Source:NCBI gene;Acc:100496302])

HSP 1 Score: 144.05 bits (362), Expect = 3.475e-36
Identity = 81/241 (33.61%), Postives = 127/241 (52.70%), Query Frame = 3 
Query:   87 CSLCDIS--CDEPYLGELLT--ESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEM 797
            C  C +S  C E + GE  T  E + ++H  CL  S  + Q+     K    I GF + DI+KE+ R ++L+C +CK R A IGC    C+RSYH  CG++   +  FTDSF  YC EH   + ++K + S  +    C  CL++                    + +V++  ++ + CC  +W HR C+    L++GL   +C  C NK ++ +  L+ G+ IP+RDA+WE+E  AFQE+
Sbjct:   14 CVFCGLSDDCAEKF-GEKKTYPEHDLTLHYYCLLLSSGIWQR----GKENEGIYGFLVCDIKKEVNRANKLRCALCKNRGASIGCVFPKCRRSYHYHCGVKKECIFQFTDSFRSYCWEH---RPVQKIVSSQTDCSSTCTICLEN--------------------VNHVSSYHILRSPCCKTSWFHRNCLQHQALSAGLFFFRCTVCNNKDIFQNEMLRMGIHIPERDASWELEENAFQEL 226
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Xenopus
Match: dnajc8 (DnaJ heat shock protein family (Hsp40) member C8 [Source:Xenbase;Acc:XB-GENE-946546])

HSP 1 Score: 65.855 bits (159), Expect = 2.643e-11
Identity = 39/123 (31.71%), Postives = 63/123 (51.22%), Query Frame = 3 
Query:   78 KRTCSLCDISCDEPYLGELLT--ESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFT---DSFTFYCKEH 431
            +R C+ C +       G LL   +++ + H NC+ FSP     +  + K  G   GF I+ ++KE+KR  R++C  CKK  A IGC    CK++YH +C  +   + I     + +  YC +H
Sbjct:    8 RRECAFCHLREQNEETGRLLKTRDNHVTAHFNCMIFSP---NAITTNDKEFG---GFDIASVKKEVKRGKRMRCSFCKKTGATIGCDVPRCKKTYHYMCAKRDLGLIIENEKEEKYLIYCADH 124
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Xenopus
Match: ENSXETT00000027447.1 (uncharacterized LOC100492657 [Source:NCBI gene;Acc:100492657])

HSP 1 Score: 62.003 bits (149), Expect = 1.124e-9
Identity = 37/130 (28.46%), Postives = 61/130 (46.92%), Query Frame = 3 
Query:   57 ISNTNTIKRTCSLCDISCDEPYLGELLTESNFSI--HENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFT---DSFTFYCKEH 431
            +SN+   +  C+ C         G+LL  S+  +  H  C+ FSP +     P  +      GF    +E EIKR  ++KC  C K    IGC  + C+++YH +C   + A+ I     +++  YC +H
Sbjct:    1 MSNSTPNRGVCAFCQQGEQNKKTGDLLQTSDGEVTAHYYCMLFSPNVITTSSPDEE----FGGFDRRTVENEIKRGRKMKCSSCNKVGGTIGCNKKSCRKTYHYMCAETNGALIIENQEEENYIIYCTKH 126
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Xenopus
Match: ENSXETT00000027460.1 (uncharacterized LOC100492657 [Source:NCBI gene;Acc:100492657])

HSP 1 Score: 62.003 bits (149), Expect = 1.412e-9
Identity = 37/130 (28.46%), Postives = 61/130 (46.92%), Query Frame = 3 
Query:   57 ISNTNTIKRTCSLCDISCDEPYLGELLTESNFSI--HENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFT---DSFTFYCKEH 431
            +SN+   +  C+ C         G+LL  S+  +  H  C+ FSP +     P  +      GF    +E EIKR  ++KC  C K    IGC  + C+++YH +C   + A+ I     +++  YC +H
Sbjct:    1 MSNSTPNRGVCAFCQQGEQNKKTGDLLQTSDGEVTAHYYCMLFSPNVITTSSPDEE----FGGFDRRTVENEIKRGRKMKCSSCNKVGGTIGCNKKSCRKTYHYMCAETNGALIIENQEEENYIIYCTKH 126
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Xenopus
Match: tgif2 (TGFB induced factor homeobox 2 [Source:Xenbase;Acc:XB-GENE-876497])

HSP 1 Score: 60.077 bits (144), Expect = 3.175e-9
Identity = 43/123 (34.96%), Postives = 59/123 (47.97%), Query Frame = 3 
Query:   78 KRTCSLCDISCDEPYLGELLTESNFSI--HENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDS---FTFYCKEH 431
            +R C  C  S  E   G+LL  SN  +  H  C+ FS  L      S      + GF I DI+KE+KR  +L C +C    A IGC  + C R+YH  C L+  A      S   +T +C++H
Sbjct:   14 QRKCGFCR-SNREKECGQLLISSNQKVAAHHRCMLFSSALVSSQSDSEN----LGGFSIEDIQKELKRGRKLMCSLCHCPGATIGCDVKSCHRTYHYHCALRDKAHIQENPSQGMYTIFCRKH 131
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Mouse
Match: G2e3 (G2/M-phase specific E3 ubiquitin ligase [Source:MGI Symbol;Acc:MGI:2444298])

HSP 1 Score: 145.976 bits (367), Expect = 3.990e-37
Identity = 77/219 (35.16%), Postives = 114/219 (52.05%), Query Frame = 3 
Query:  147 NFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYE 803
            NFS+H  CL  S  + Q+     K +  + GF I DI KE++R S+LKC VCKK  A IGC    CKRSYHL CGLQ   +  FTD+F  +C +H   Q                       A T N+  +S+P      F+  + T +++ + CC  AW HR C+    + +G+   +C  C N  ++    L+ G+ IP++DA+WE+E  A+QE+ +
Sbjct:   37 NFSVHYYCLLMSSGIWQR----GKEEEGVYGFLIEDIRKEVQRASKLKCTVCKKNGASIGCVVPTCKRSYHLPCGLQKECIFQFTDNFASFCWKHRPVQ-----------------------AITSNKYSSSLPCTICLEFVEPIPTYNILQSPCCKNAWFHRDCLQVQAINAGVFFFRCTLCNNTDIFQKEMLRMGIHIPEKDASWELEENAYQELLQ 228
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Mouse
Match: G2e3 (G2/M-phase specific E3 ubiquitin ligase [Source:MGI Symbol;Acc:MGI:2444298])

HSP 1 Score: 145.976 bits (367), Expect = 7.879e-37
Identity = 77/219 (35.16%), Postives = 114/219 (52.05%), Query Frame = 3 
Query:  147 NFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYE 803
            NFS+H  CL  S  + Q+     K +  + GF I DI KE++R S+LKC VCKK  A IGC    CKRSYHL CGLQ   +  FTD+F  +C +H   Q                       A T N+  +S+P      F+  + T +++ + CC  AW HR C+    + +G+   +C  C N  ++    L+ G+ IP++DA+WE+E  A+QE+ +
Sbjct:   37 NFSVHYYCLLMSSGIWQR----GKEEEGVYGFLIEDIRKEVQRASKLKCTVCKKNGASIGCVVPTCKRSYHLPCGLQKECIFQFTDNFASFCWKHRPVQ-----------------------AITSNKYSSSLPCTICLEFVEPIPTYNILQSPCCKNAWFHRDCLQVQAINAGVFFFRCTLCNNTDIFQKEMLRMGIHIPEKDASWELEENAYQELLQ 228
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Mouse
Match: G2e3 (G2/M-phase specific E3 ubiquitin ligase [Source:MGI Symbol;Acc:MGI:2444298])

HSP 1 Score: 145.976 bits (367), Expect = 7.981e-37
Identity = 77/219 (35.16%), Postives = 114/219 (52.05%), Query Frame = 3 
Query:  147 NFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYE 803
            NFS+H  CL  S  + Q+     K +  + GF I DI KE++R S+LKC VCKK  A IGC    CKRSYHL CGLQ   +  FTD+F  +C +H   Q                       A T N+  +S+P      F+  + T +++ + CC  AW HR C+    + +G+   +C  C N  ++    L+ G+ IP++DA+WE+E  A+QE+ +
Sbjct:   37 NFSVHYYCLLMSSGIWQR----GKEEEGVYGFLIEDIRKEVQRASKLKCTVCKKNGASIGCVVPTCKRSYHLPCGLQKECIFQFTDNFASFCWKHRPVQ-----------------------AITSNKYSSSLPCTICLEFVEPIPTYNILQSPCCKNAWFHRDCLQVQAINAGVFFFRCTLCNNTDIFQKEMLRMGIHIPEKDASWELEENAYQELLQ 228
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Mouse
Match: Phf7 (PHD finger protein 7 [Source:MGI Symbol;Acc:MGI:1919088])

HSP 1 Score: 139.428 bits (350), Expect = 8.759e-37
Identity = 88/275 (32.00%), Postives = 134/275 (48.73%), Query Frame = 3 
Query:  111 DEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPE-CAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGFQMSPDGNPVLPVTHIQGDKGSH 932
            D   LGE L + N  +H  CL  S  L Q+  P+   +G + GF   DI++E  R S+  CFVCKK+ A I C N+ C +++HL CG +   +  F   +  YC++H   Q++ +     G++  E C  C ++++ T  E + S                      CC++A  HR C+ K+   S  H  KCP C N+  +    L+ G+ IPDRDAAWE+EPGAF E+Y+     + D  I +       F+   +G   L +    G  G+H
Sbjct:   42 DPEKLGEFLQKDNLCVHYFCLILSSRLPQKGQPN---RG-LHGFMPEDIKREAVRASKKICFVCKKKGAAIRCQNDQCVQNFHLPCGQERGCLSQFFGEYKSYCRKHRPTQNIHQ-----GSLGEESCVLCCENLSRTSVENIQSP---------------------CCSQAIYHRKCIQKYAHTSAKHFFKCPQCNNREEFPQEMLRMGIHIPDRDAAWELEPGAFSELYQRYR--HCDAPICLYEQGRDSFE--DEGRWRLILCATCGSHGTH 282
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Mouse
Match: Phf7 (PHD finger protein 7 [Source:MGI Symbol;Acc:MGI:1919088])

HSP 1 Score: 108.997 bits (271), Expect = 2.828e-26
Identity = 69/222 (31.08%), Postives = 107/222 (48.20%), Query Frame = 3 
Query:  111 DEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPE-CAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEM 773
            D   LGE L + N  +H  CL  S  L Q+  P+   +G + GF   DI++E  R S+  CFVCKK+ A I C N+ C +++HL CG +   +  F   +  YC++H   Q++ +     G++  E C  C ++++ T  E + S                      CC++A  HR C+ K+   S  H  KCP C N+  +    L+ G+ IPDR   W +
Sbjct:   42 DPEKLGEFLQKDNLCVHYFCLILSSRLPQKGQPN---RG-LHGFMPEDIKREAVRASKKICFVCKKKGAAIRCQNDQCVQNFHLPCGQERGCLSQFFGEYKSYCRKHRPTQNIHQ-----GSLGEESCVLCCENLSRTSVENIQS---------------------PCCSQAIYHRKCIQKYAHTSAKHFFKCPQCNNREEFPQEMLRMGIHIPDRR--WRL 231
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. UniProt/SwissProt
Match: sp|Q5RJY2|G2E3_MOUSE (G2/M phase-specific E3 ubiquitin-protein ligase OS=Mus musculus OX=10090 GN=G2e3 PE=2 SV=2)

HSP 1 Score: 145.976 bits (367), Expect = 5.512e-36
Identity = 77/219 (35.16%), Postives = 114/219 (52.05%), Query Frame = 3 
Query:  147 NFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYE 803
            NFS+H  CL  S  + Q+     K +  + GF I DI KE++R S+LKC VCKK  A IGC    CKRSYHL CGLQ   +  FTD+F  +C +H   Q                       A T N+  +S+P      F+  + T +++ + CC  AW HR C+    + +G+   +C  C N  ++    L+ G+ IP++DA+WE+E  A+QE+ +
Sbjct:   37 NFSVHYYCLLMSSGIWQR----GKEEEGVYGFLIEDIRKEVQRASKLKCTVCKKNGASIGCVVPTCKRSYHLPCGLQKECIFQFTDNFASFCWKHRPVQ-----------------------AITSNKYSSSLPCTICLEFVEPIPTYNILQSPCCKNAWFHRDCLQVQAINAGVFFFRCTLCNNTDIFQKEMLRMGIHIPEKDASWELEENAYQELLQ 228
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. UniProt/SwissProt
Match: sp|Q9DAG9|PHF7_MOUSE (PHD finger protein 7 OS=Mus musculus OX=10090 GN=Phf7 PE=1 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 6.127e-36
Identity = 88/275 (32.00%), Postives = 134/275 (48.73%), Query Frame = 3 
Query:  111 DEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPE-CAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGFQMSPDGNPVLPVTHIQGDKGSH 932
            D   LGE L + N  +H  CL  S  L Q+  P+   +G + GF   DI++E  R S+  CFVCKK+ A I C N+ C +++HL CG +   +  F   +  YC++H   Q++ +     G++  E C  C ++++ T  E + S                      CC++A  HR C+ K+   S  H  KCP C N+  +    L+ G+ IPDRDAAWE+EPGAF E+Y+     + D  I +       F+   +G   L +    G  G+H
Sbjct:   42 DPEKLGEFLQKDNLCVHYFCLILSSRLPQKGQPN---RG-LHGFMPEDIKREAVRASKKICFVCKKKGAAIRCQNDQCVQNFHLPCGQERGCLSQFFGEYKSYCRKHRPTQNIHQ-----GSLGEESCVLCCENLSRTSVENIQSP---------------------CCSQAIYHRKCIQKYAHTSAKHFFKCPQCNNREEFPQEMLRMGIHIPDRDAAWELEPGAFSELYQRYR--HCDAPICLYEQGRDSFE--DEGRWRLILCATCGSHGTH 282
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. UniProt/SwissProt
Match: sp|Q9BWX1|PHF7_HUMAN (PHD finger protein 7 OS=Homo sapiens OX=9606 GN=PHF7 PE=1 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 5.169e-34
Identity = 88/275 (32.00%), Postives = 129/275 (46.91%), Query Frame = 3 
Query:  111 DEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPE-CAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGFQMSPDGNPVLPVTHIQGDKGSH 932
            D   LGE L + N S+H  CL  S  L Q+       +GF  GF   DI+KE  R SR  CFVCKK+ A I C  + C R++HL CG +   +  F   +  +C +H   Q+++      G++  E C  C + ++    E + S                      CC++A  HR C+ K+   S  H  KCP C N+  +    L+ G+ IPDRDAAWE+EPGAF ++Y+     + D  I +       F+   +G   L +    G  G+H
Sbjct:   42 DPEKLGEFLQKDNISVHYFCLILSSKLPQR---GQSNRGF-HGFLPEDIKKEAARASRKICFVCKKKGAAINCQKDQCLRNFHLPCGQERGCLSQFFGEYKSFCDKHRPTQNIQH-----GHVGEESCILCCEDLSQQSVENIQSP---------------------CCSQAIYHRKCIQKYAHTSAKHFFKCPQCNNRKEFPQEMLRMGIHIPDRDAAWELEPGAFSDLYQRYQ--HCDAPICLYEQGRDSFE--DEGRWCLILCATCGSHGTH 282
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. UniProt/SwissProt
Match: sp|Q6AXW4|PHF7_RAT (PHD finger protein 7 OS=Rattus norvegicus OX=10116 GN=Phf7 PE=2 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 6.571e-34
Identity = 79/233 (33.91%), Postives = 116/233 (49.79%), Query Frame = 3 
Query:  111 DEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLD--SMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYE 803
            D   LGE L + N  +H  CL  S  L Q+  P+   +G + GF   DI+KE  R SR  CFVCK++ A I C  + C +++HL CG +   +  F   +  YC +H   Q++ ++     +    C  C +  S A+ EN                       + + CC++A  HR C+ K+   S  H  KCP C N+  +    L+ G+ IPDRDAAWE+EPGAF E+Y+
Sbjct:   42 DPEKLGEFLQKDNLCVHYFCLILSSKLPQKGQPN---RG-LHGFMPEDIKKEAARASRKVCFVCKRKGAAIRCQKDQCVQNFHLPCGQERGCLSQFFGEYKSYCGKHRPTQNIHQR-----SFGESCVLCCEDLSRASVEN-----------------------IRSPCCSQAIYHRKCIQKYAHTSAKHFFKCPQCNNREEFPQEMLRMGIHIPDRDAAWELEPGAFSELYQ 242
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. UniProt/SwissProt
Match: sp|Q5F4A1|G2E3_CHICK (G2/M phase-specific E3 ubiquitin-protein ligase OS=Gallus gallus OX=9031 GN=G2E3 PE=2 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 2.969e-32
Identity = 81/243 (33.33%), Postives = 119/243 (48.97%), Query Frame = 3 
Query:   87 CSLCDIS--CDEPYLGELLT--ESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYE 803
            C LC  +  C E Y GE  T  E N ++H  CL  S  + Q+ + +    GF+    I DI KE+ R +RL C +C+K+ A IGC    CKRSYH  CGLQ   +  F + F  YC EH   Q    + ES      +C  CLD                     + ++   SV+ + CC   W HR C+    L++G+   +C  C NK  +    L+ G+ IP++DA+WE+E  A+Q++ +
Sbjct:   13 CVLCGWTDNCPEKY-GEKRTYVEYNLTLHNYCLLMSSGIWQRGEENEGVDGFL----IEDIRKEVNRAARLMCNICRKKGASIGCVAPKCKRSYHFPCGLQKECVFQFMEDFRSYCWEHKPVQIFSDK-ES--REPSQCTICLD--------------------LVEHLPLYSVLRSPCCKNTWFHRECLQYQALSAGIFFFRCAVCNNKDKFQKEMLRMGIHIPEKDASWELEDNAYQDLLQ 227
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. TrEMBL
Match: A0A430QFT9 (G2/M phase-specific E3 ubiquitin-protein ligase OS=Schistosoma bovis OX=6184 GN=DC041_0009525 PE=4 SV=1)

HSP 1 Score: 157.918 bits (398), Expect = 3.349e-39
Identity = 94/330 (28.48%), Postives = 155/330 (46.97%), Query Frame = 3 
Query:   87 CSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEV---------------LASVPDNEWDLFMRNVATRSV----------MHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDE-----AITISSDSDTGFQMSPDGNPVLPVTHIQGDKGSHLISDGDAA-----LPVLS 971
            C  C    D P LG ++ ++ F +H NC+F +  L Q  + +     FI+GF +S I  E++R   LKC VC+   A +GC    C  ++HL C +++  + IF  +F  YC+ HA RQ        C +   E      S    +N V               ++ +P    D F+     + +          +H  CC  +W+HR C+A   +++ LH++KCP C N+ ++I + +  G+W+PD+DAAWE+EPGA+ ++   +   ++DE       T SS  D       + +    VT   G +  +  S G  +     +PVLS
Sbjct:   14 CIFCSEVDDYPLLGPIIEKNGFKVHTNCIFAASGLSQTNNENESPDDFIVGFAVSSILNELRRGRSLKCSVCRLPGACVGCVVNTCHTTFHLPCLIKAQGITIFEGNFPSYCRRHANRQSFYLLFNRCTSTW-ELPGSSSSEPEEQNPVQQHKTPKESVTLPSSMSHIPGWLTDYFLSLAPEKRLPVFEAWKHHTIHGLCCPLSWMHRNCIAGFAVSAALHYLKCPYCANREIFIRSIIDTGIWVPDQDAAWELEPGAYADLVPISECSSNDEPQSNDTSTTSSQCDCNRWSQKNVSHAAHVTQTAGVQVEYASSSGSPSTDQERVPVLS 342
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. TrEMBL
Match: A0A210Q9Q5 (G2/M phase-specific E3 ubiquitin-protein ligase OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT10383 PE=4 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 4.520e-39
Identity = 81/242 (33.47%), Postives = 131/242 (54.13%), Query Frame = 3 
Query:   87 CSLCDISCD-EPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQ--DLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYE 803
            C+ C+++ D E   G+++ + + ++H  C+ FS  L Q    + + +G I GF   DI KE++R +RL+C  CK++ A IGC    C+R +H  CG     M  + DSF  +C++H  RQ   +  ++   G     CA C+ S+                       A+   + A CC  +W HR+C+ KH L +GL+  KCP C NK ++ +  LK+G+++PD+DAAWE EP AF+++ E
Sbjct:   56 CAFCNVAEDNEEVFGKMIKKCDLTVHYFCMLFSSGLSQ--GGNSEEEG-IFGFMPEDIMKEVRRGARLRCTYCKQKGATIGCVVGACRRVFHFGCGRSGGTMHQYYDSFRSFCEDHRPRQTVSVSDRLAFYGTANTTCAICMTSVETR--------------------ASNETLRAPCCRNSWFHRSCIVKHALTAGLYFFKCPLCNNKEIFQEEMLKFGVYLPDQDAAWETEPNAFRDLLE 274
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. TrEMBL
Match: A0A1S3J2F4 (uncharacterized protein LOC106169620 OS=Lingula unguis OX=7574 GN=LOC106169620 PE=4 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 3.295e-36
Identity = 83/255 (32.55%), Postives = 128/255 (50.20%), Query Frame = 3 
Query:   60 SNTNTIKRTCSLCDISCD-EPYLGELLTESNFSIHENCLFFSPVLHQ----QLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDL--RKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYE 803
            S   +    C  C+ SC+ E  LG+ L   N ++H  C+ F+  L Q    +LD        ILGF   DI KE++R  +LKC  CK++ A IGC    C++ +H  CG  +  +  F  +++ YC+EH  RQ +    ++   G     CA C+ ++ A                     A+   + A CC  AW HR+C+ +    SG++  KCP C NK  +    L++G++IPD+DA WE+EP A+QE+ E
Sbjct:    8 SGAKSTNGACVFCNSSCENEELLGKKLARKNLTVHYFCMLFASGLFQSGKDELD-------GILGFLPRDIIKEVQRGKKLKCCFCKEKGATIGCVVRCCRQVFHFGCGRDNGVLNQFFGTYSSYCEEHRPRQTVPTSDRLAFHGTAKAVCAICMCAVEAR--------------------ASNETLRAPCCKNAWFHRSCIQRQATCSGVYFFKCPLCNNKDYFQHEMLEFGIYIPDQDAEWELEPDAYQELLE 235
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. TrEMBL
Match: G3W5T1 (PHD finger protein 7 OS=Sarcophilus harrisii OX=9305 GN=PHF7 PE=4 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 4.505e-35
Identity = 90/274 (32.85%), Postives = 132/274 (48.18%), Query Frame = 3 
Query:  111 DEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGFQMSPDGNPVLPVTHIQGDKGSH 932
            D   LGE L   N  IH  CL  S  L Q+   S    GF  GF   DI+KE  R +R  CFVCKK+ A I C  E C+R++HL CG +   +  F   +  +C++H   QD++ +  +      +C  C +S++   N+ + S                      CC+R   HR C+ K+  +S  H  KCP C N+  +    L+ G+ IPDRDAAWE+EPGAF E+Y+     + D  + +  D    F+   +G   L +    G +G+H
Sbjct:   18 DPEKLGEFLQRDNLRIHYFCLILSSGLPQRGRAS---DGF-HGFLPEDIKKETSRAARKTCFVCKKKGAAIFCQKERCRRNFHLPCGRERGCISQFFGEYKSFCEKHRPTQDIQPEKPA----EEDCILCCESLSGGNNDNIQSP---------------------CCSRMIYHRKCIQKYAHSSAKHFFKCPQCNNREEFPKEMLRMGIHIPDRDAAWELEPGAFSELYQRHQ--HCDAPVCLYKDGRDNFEN--EGRWSLILCTTCGSQGTH 258
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. TrEMBL
Match: G3W5T0 (PHD finger protein 7 OS=Sarcophilus harrisii OX=9305 GN=PHF7 PE=4 SV=1)

HSP 1 Score: 145.206 bits (365), Expect = 6.328e-35
Identity = 90/274 (32.85%), Postives = 132/274 (48.18%), Query Frame = 3 
Query:  111 DEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGFQMSPDGNPVLPVTHIQGDKGSH 932
            D   LGE L   N  IH  CL  S  L Q+   S    GF  GF   DI+KE  R +R  CFVCKK+ A I C  E C+R++HL CG +   +  F   +  +C++H   QD++ +  +      +C  C +S++   N+ + S                      CC+R   HR C+ K+  +S  H  KCP C N+  +    L+ G+ IPDRDAAWE+EPGAF E+Y+     + D  + +  D    F+   +G   L +    G +G+H
Sbjct:   42 DPEKLGEFLQRDNLRIHYFCLILSSGLPQRGRAS---DGF-HGFLPEDIKKETSRAARKTCFVCKKKGAAIFCQKERCRRNFHLPCGRERGCISQFFGEYKSFCEKHRPTQDIQPEKPA----EEDCILCCESLSGGNNDNIQSP---------------------CCSRMIYHRKCIQKYAHSSAKHFFKCPQCNNREEFPKEMLRMGIHIPDRDAAWELEPGAFSELYQRHQ--HCDAPVCLYKDGRDNFEN--EGRWSLILCTTCGSQGTH 282
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Cavefish
Match: g2e3 (G2/M-phase specific E3 ubiquitin protein ligase [Source:ZFIN;Acc:ZDB-GENE-030616-400])

HSP 1 Score: 119.783 bits (299), Expect = 2.249e-28
Identity = 84/289 (29.07%), Postives = 138/289 (47.75%), Query Frame = 3 
Query:   87 CSLCDI--SCDEPYLGELLT--ESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNI--VPECAA-CLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGFQMSPDGNPVLPVTHIQGDKGSH 932
            C LC+   +C + Y GE  T  + N ++H  CL  S  + Q+     K    I GF + DI++EI+R SRL+C  CKK  A +GC  + C++  H+ CG++   +  FTDSF  +CK+H       +  ++C ++  +P C + CL+ +                      V + SV+    C  +W HR C+     ++     KC  C NK  +    L+ G+ IP+RDA+WE+E  A+ E+ E      H +A+  S +   G+  +  G   +      G +G+H
Sbjct:   16 CQLCNRRDNCPDKY-GEKFTLKKHNITVHYFCLLMSSGIFQR----GKEHEGIHGFLVDDIKQEIRRSSRLRCVACKKVGASVGCFIKSCRQMVHMPCGVEQQFIFQFTDSFPSFCKKH-------RPTQTCSSVPTLPVCCSICLEHIEP--------------------VLSYSVLKCPACHGSWFHRNCVQYQAHSAAKFFFKCTLCNNKDQFQQEMLRMGIHIPERDASWELEDNAYGELLE---VYQHCDAVKCSCEKGRGYS-AETGVFEIVRCKFCGSRGTH 268
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Cavefish
Match: ENSAMXT00000011361.2 (pep primary_assembly:Astyanax_mexicanus-2.0:19:25668253:25673597:1 gene:ENSAMXG00000011058.2 transcript:ENSAMXT00000011361.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 73.559 bits (179), Expect = 2.703e-14
Identity = 40/118 (33.90%), Postives = 60/118 (50.85%), Query Frame = 3 
Query:   87 CSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDS---FTFYCKEH 431
            C LC    +    G LL++ + S HENC+ FS  ++ +  PS      + GF + D++ E  R  +LKC  C+K  A +GC  + CKRSYH  C +   A  +   S   +  YC +H
Sbjct:   15 CILCKTDDENRITGPLLSKEDISAHENCMVFSSGIYCKNSPSFDD---LCGFDVEDVKAECSRGKKLKCHYCRKSGATVGCEEKKCKRSYHYPCAIADRARCVEKKSRGLYMLYCPKH 129
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Cavefish
Match: ENSAMXT00000044632.1 (pep primary_assembly:Astyanax_mexicanus-2.0:19:25668253:25673597:1 gene:ENSAMXG00000011058.2 transcript:ENSAMXT00000044632.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 73.559 bits (179), Expect = 2.703e-14
Identity = 40/118 (33.90%), Postives = 60/118 (50.85%), Query Frame = 3 
Query:   87 CSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDS---FTFYCKEH 431
            C LC    +    G LL++ + S HENC+ FS  ++ +  PS      + GF + D++ E  R  +LKC  C+K  A +GC  + CKRSYH  C +   A  +   S   +  YC +H
Sbjct:   15 CILCKTDDENRITGPLLSKEDISAHENCMVFSSGIYCKNSPSFDD---LCGFDVEDVKAECSRGKKLKCHYCRKSGATVGCEEKKCKRSYHYPCAIADRARCVEKKSRGLYMLYCPKH 129
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Cavefish
Match: phf6 (PHD finger protein 6 [Source:NCBI gene;Acc:103026263])

HSP 1 Score: 65.855 bits (159), Expect = 3.339e-11
Identity = 41/122 (33.61%), Postives = 60/122 (49.18%), Query Frame = 3 
Query:   81 RTCSLCDISCDEPYLGELLTESNFSI--HENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDS---FTFYCKEH 431
            R C+ C  + D+   G+LL   N  +  H  C+ FS  L      S      I GF + D++KEIKR ++L C  C++  A IGC  + C+R+YH  C L   A      S   +  YC++H
Sbjct:   13 RKCAFCRTNRDKE-CGQLLVSDNQRVAAHHKCMLFSSALVTSHSDSEN----IGGFSVEDVKKEIKRGNKLMCSSCRRPGATIGCDVKTCRRTYHYYCALWDKAQTKENPSQGIYLVYCRKH 129

HSP 2 Score: 53.9138 bits (128), Expect = 2.312e-7
Identity = 38/122 (31.15%), Postives = 54/122 (44.26%), Query Frame = 3 
Query:   87 CSLCDISCDEPYLGELLTESN---FSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDS---FTFYCKEHA 434
            C  C    +E     +L   N    + H  C+ FS    Q    S    G    F I  + +EIKR  R+KC +C +  A IGC  + C ++YH  CG+Q  A  I   +   +  YCK H+
Sbjct:  210 CGFCHAGDEENETRGVLHSDNGKKVAAHYKCMLFSSGTVQLTTSSRAEFG---NFEIKTVIQEIKRGKRMKCTLCGQLGATIGCEIKACVKTYHYHCGVQDKAKYIENMARGIYKLYCKNHS 328
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Cavefish
Match: phf6 (PHD finger protein 6 [Source:NCBI gene;Acc:103026263])

HSP 1 Score: 62.003 bits (149), Expect = 4.879e-10
Identity = 36/100 (36.00%), Postives = 52/100 (52.00%), Query Frame = 3 
Query:   81 RTCSLCDISCDEPYLGELLTESNFSI--HENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGL 374
            R C+ C  + D+   G+LL   N  +  H  C+ FS  L      S      I GF + D++KEIKR ++L C  C++  A IGC  + C+R+YH  C L
Sbjct:   15 RKCAFCRTNRDKE-CGQLLVSDNQRVAAHHKCMLFSSALVTSHSDSEN----IGGFSVEDVKKEIKRGNKLMCSSCRRPGATIGCDVKTCRRTYHYYCAL 109

HSP 2 Score: 53.9138 bits (128), Expect = 1.966e-7
Identity = 38/122 (31.15%), Postives = 54/122 (44.26%), Query Frame = 3 
Query:   87 CSLCDISCDEPYLGELLTESN---FSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDS---FTFYCKEHA 434
            C  C    +E     +L   N    + H  C+ FS    Q    S    G    F I  + +EIKR  R+KC +C +  A IGC  + C ++YH  CG+Q  A  I   +   +  YCK H+
Sbjct:  190 CGFCHAGDEENETRGVLHSDNGKKVAAHYKCMLFSSGTVQLTTSSRAEFG---NFEIKTVIQEIKRGKRMKCTLCGQLGATIGCEIKACVKTYHYHCGVQDKAKYIENMARGIYKLYCKNHS 308
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007094.1 (pep scaffold:Pmarinus_7.0:GL483693:43:11634:1 gene:ENSPMAG00000006416.1 transcript:ENSPMAT00000007094.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 112.079 bits (279), Expect = 2.662e-28
Identity = 72/214 (33.64%), Postives = 112/214 (52.34%), Query Frame = 3 
Query:  168 CLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHA-IRQDLRKQIESCGNIVPE-CAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCM--AKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEM 797
            CL FS  L QQ + S   +GF  GF   DI KE KR SRLKC +CK++ A IGC    C+R +HL CG++ + +  F  +F  +CKEH  I+ ++R +     ++ P+ C  CL+ +                     ++++   + +  C   + HR+C+   +  L SGL   +CP C N   +    L+ G+ IP+RDA+WE +  AFQE+
Sbjct:    1 CLIFSSGLCQQGNDS---EGFY-GFLPDDIIKEFKRASRLKCMLCKQKGAAIGCNLTSCRRKFHLPCGIKLHCLFQFHGTFPSFCKEHRPIQLEMRGR----ADVEPQTCVLCLEVID--------------------DLSSFDTLRSASCCNTFFHRSCIQQQQQALHSGLFFFRCPICNNNEKFQAEMLRMGIHIPERDASWERDENAFQEL 186
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Nematostella
Match: EDO31769 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SWZ5])

HSP 1 Score: 119.013 bits (297), Expect = 2.326e-30
Identity = 84/287 (29.27%), Postives = 125/287 (43.55%), Query Frame = 3 
Query:  111 DEPYLGELLTES--NFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHA-IRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGFQMSPDGNPVLPVTHIQGDKGSHLISDGDAALP 962
            DE   G+ L++   N ++H+ CL F+  L Q        +GF  GF   DI+ E+KR  RLKC  C    A IGC    C + +H  CG    A+  F  SF  +C+ H   ++DL+    +     P C   +D+                 DL+         + + CC R+W HR C+ +   ASG+H  +C  C N   + D  L+ G+ IPDRDA WE    AF ++ E     + D  +        G      G   + +  + G  G+H I  G  A+P
Sbjct:   11 DEDKYGKFLSKEGENITVHQYCLLFASGLAQN---GGDDEGFD-GFLCKDIDTELKRAKRLKCSYCHVSGASIGCVAVKCSKKFHFGCGRDKQALFQFFGSFNSFCRSHRPTQKDLQCNHSAGAETCPICLMSVDAH----------------DLY-------ETLKSPCCKRSWFHRECVQRQATASGIHFFRCAVCNNIQDFQDEMLRMGIHIPDRDAVWE-SGDAFADLLETYNRCDADVCLCKKGREHGG----RHGKWKIRLCDVCGQCGTHKICQGWVAMP 265
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Nematostella
Match: EDO30859 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SYF7])

HSP 1 Score: 54.299 bits (129), Expect = 7.534e-8
Identity = 34/126 (26.98%), Postives = 57/126 (45.24%), Query Frame = 3 
Query:   72 TIKRTCSLCD-ISCDEPYLGELLTESNFS--IHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIR 440
            T  R C LC+ +  DEP     L  S     +H NC  +S  + +  +            R+ +++  + R   +KC +C +  A +GCC   C  +YH +CG   +A  ++ D    YC +H+ R
Sbjct:  472 TDTRKCQLCNRLGDDEPTRAGRLLYSALDEWVHINCGLWSAEVFEDDEG-----------RLQNVQAAVTRGKMMKCELCGEAGATVGCCENRCPMNYHFMCG--RDAEAVYQDDKKVYCAQHSFR 584
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Medaka
Match: g2e3 (G2/M-phase specific E3 ubiquitin protein ligase [Source:NCBI gene;Acc:101167753])

HSP 1 Score: 135.191 bits (339), Expect = 2.599e-33
Identity = 80/249 (32.13%), Postives = 125/249 (50.20%), Query Frame = 3 
Query:   66 TNTIKRTCSLCDISCDEPYL-GELLT--ESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYE 803
            T  ++  C LC I+ D+P L GE +T  +   ++H  CL  S  ++Q+     K    ILGF + DI +E++R S L C  CKK+ A +GC    C++  H  CG   + +  FT+ F  YCK HA  Q L   ++   ++   C+ CLDS+                      V + SV+    C  +W HR C+ +   ++GL   KC  C NK  +    L+ G++IP+RDA+WE+E  A+ ++ E
Sbjct:   11 TTVLQDCCVLCRINDDDPALFGEKVTLEQQKLTVHYLCLLTSCGVYQR----GKENEGILGFLVDDIRQEVRRSSHLTCVSCKKKGACVGCNVRSCRQMVHFPCGRNLSFISQFTEPFPSYCKNHAPSQSL--SVDPNISLPQSCSVCLDSLDP--------------------VLSYSVLKCPSCHASWFHRDCVQRQAFSAGLFFFKCTLCNNKDRFQQEMLRMGIYIPERDASWELESDAYSDLLE 233
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Medaka
Match: phf6 (PHD finger protein 6 [Source:NCBI gene;Acc:101160988])

HSP 1 Score: 62.003 bits (149), Expect = 3.815e-10
Identity = 41/128 (32.03%), Postives = 63/128 (49.22%), Query Frame = 3 
Query:   72 TIKRTCSLCDISCDEPYLGELLTESNFSI--HENCLFFSPVL---HQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDS---FTFYCKEH 431
            T ++ C+ C  + D+   G+LL   +  +  H  C+ FS  L   H   D        I GF I D++KEIKR ++L C  C +  A IGC  + C+R+YH  C ++  A      S   +  YC++H
Sbjct:   12 TRQQKCAFCRANRDKE-CGQLLVSDSQKVAAHHKCMLFSSALVTSHADGDT-------IGGFSIEDVKKEIKRGNKLMCSSCHRPGATIGCDVKTCRRTYHYYCAVKDKAQVKENPSQGIYLVYCRKH 131
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Medaka
Match: phf6 (PHD finger protein 6 [Source:NCBI gene;Acc:101160988])

HSP 1 Score: 62.003 bits (149), Expect = 4.498e-10
Identity = 41/128 (32.03%), Postives = 63/128 (49.22%), Query Frame = 3 
Query:   72 TIKRTCSLCDISCDEPYLGELLTESNFSI--HENCLFFSPVL---HQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDS---FTFYCKEH 431
            T ++ C+ C  + D+   G+LL   +  +  H  C+ FS  L   H   D        I GF I D++KEIKR ++L C  C +  A IGC  + C+R+YH  C ++  A      S   +  YC++H
Sbjct:   10 TRQQKCAFCRANRDKE-CGQLLVSDSQKVAAHHKCMLFSSALVTSHADGDT-------IGGFSIEDVKKEIKRGNKLMCSSCHRPGATIGCDVKTCRRTYHYYCAVKDKAQVKENPSQGIYLVYCRKH 129

HSP 2 Score: 55.0694 bits (131), Expect = 7.523e-8
Identity = 39/122 (31.97%), Postives = 54/122 (44.26%), Query Frame = 3 
Query:   87 CSLCDISCDEPYLGELLTESN---FSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDS---FTFYCKEHA 434
            C  C    +E     LL   N    + H  C+ FS    Q    S    G    F +  + +EIKR  R+KC +C +  A IGC  + C ++YH  CGLQ  A  I   +   +  YCK H+
Sbjct:  210 CGFCHAGEEENETRGLLHTDNSKKVAAHYKCMLFSSGTVQLTTTSRAEFG---NFDVKTVIQEIKRGKRMKCTLCGQLGATIGCEIKACVKTYHYHCGLQDKAKYIENMARGIYKLYCKNHS 328
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Planmine SMEST
Match: SMESG000026497.1 (SMESG000026497.1)

HSP 1 Score: 961.059 bits (2483), Expect = 0.000e+0
Identity = 476/491 (96.95%), Postives = 480/491 (97.76%), Query Frame = 3 
Query:   57 ISNTNTIKRTCSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGFQMSPDGNPVLPVTHIQGDKGSHLISDGDAALPVLSGGNAVLLVTLDGDSDVPISPDATGRDAAVSITSGNAAAIPVSTPAVNDENKQPVTTSSPSRSFPVGKMQSDIRKFFASSQPITSCTNLQKSNVSSKLTRKRALTSNSGASPVFIXXXXXXXXXXXXPMKTIPLVNGICSKCSRSLVCYSVNNSDNAKLVLSPKNNVKEPIKLSPENNYICIYCELL 1529
            +SNTNTIKRTCSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKK+KAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATE+EVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCL SGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGF MS DGNPV PVTHIQGD GSHLISDGDAALPVLSGGNAVLLVTLDGDSDVPISPDATGRDAAVSI SGNAAAIPVSTPAVNDENKQPVTTSSPSRSFPVGKMQSDIRKFFASSQP TSCTNLQKSNVSSK TRKRALTSNSG SPV IKRRRV SKKAKKP+KTIPLVNGICSKCSRSLVCYSVNNSDNAKLVLSPKNNVKEPIKLSPENNYICIYCELL
Sbjct:  124 VSNTNTIKRTCSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKKKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATESEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLVSGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGFPMSSDGNPVPPVTHIQGDIGSHLISDGDAALPVLSGGNAVLLVTLDGDSDVPISPDATGRDAAVSIISGNAAAIPVSTPAVNDENKQPVTTSSPSRSFPVGKMQSDIRKFFASSQPTTSCTNLQKSNVSSKSTRKRALTSNSGPSPVSIKRRRVCSKKAKKPIKTIPLVNGICSKCSRSLVCYSVNNSDNAKLVLSPKNNVKEPIKLSPENNYICIYCELL 614
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Planmine SMEST
Match: SMESG000026497.1 (SMESG000026497.1)

HSP 1 Score: 960.674 bits (2482), Expect = 0.000e+0
Identity = 477/491 (97.15%), Postives = 480/491 (97.76%), Query Frame = 3 
Query:   57 ISNTNTIKRTCSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGFQMSPDGNPVLPVTHIQGDKGSHLISDGDAALPVLSGGNAVLLVTLDGDSDVPISPDATGRDAAVSITSGNAAAIPVSTPAVNDENKQPVTTSSPSRSFPVGKMQSDIRKFFASSQPITSCTNLQKSNVSSKLTRKRALTSNSGASPVFIXXXXXXXXXXXXPMKTIPLVNGICSKCSRSLVCYSVNNSDNAKLVLSPKNNVKEPIKLSPENNYICIYCELL 1529
            ISNTNTIKRTCSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKK+KAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATE+EVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCL SGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGF MS DGNPV PVTHIQGD GSHLISDGDAALPVLSGGNAVLLVTLDGDSDVPISPDATGRDAAVSI SGNAAAIPVSTPAVNDENKQPVTTSSPSRSFPVGKMQSDIRKFFASSQP TSCTNLQKSNVSSK TRKRALTSNSG SPV IKRRRV SKKAKKP+KTIPLVNGICSKCSRSLVCYSVNNSDNAKLVLSPKNNVKEPIKLSPENNYICIYCELL
Sbjct:   39 ISNTNTIKRTCSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKKKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATESEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLVSGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGFPMSSDGNPVPPVTHIQGDIGSHLISDGDAALPVLSGGNAVLLVTLDGDSDVPISPDATGRDAAVSIISGNAAAIPVSTPAVNDENKQPVTTSSPSRSFPVGKMQSDIRKFFASSQPTTSCTNLQKSNVSSKSTRKRALTSNSGPSPVSIKRRRVCSKKAKKPIKTIPLVNGICSKCSRSLVCYSVNNSDNAKLVLSPKNNVKEPIKLSPENNYICIYCELL 529
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Planmine SMEST
Match: SMESG000026497.1 (SMESG000026497.1)

HSP 1 Score: 960.288 bits (2481), Expect = 0.000e+0
Identity = 476/491 (96.95%), Postives = 480/491 (97.76%), Query Frame = 3 
Query:   57 ISNTNTIKRTCSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGFQMSPDGNPVLPVTHIQGDKGSHLISDGDAALPVLSGGNAVLLVTLDGDSDVPISPDATGRDAAVSITSGNAAAIPVSTPAVNDENKQPVTTSSPSRSFPVGKMQSDIRKFFASSQPITSCTNLQKSNVSSKLTRKRALTSNSGASPVFIXXXXXXXXXXXXPMKTIPLVNGICSKCSRSLVCYSVNNSDNAKLVLSPKNNVKEPIKLSPENNYICIYCELL 1529
            +SNTNTIKRTCSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKK+KAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATE+EVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCL SGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGF MS DGNPV PVTHIQGD GSHLISDGDAALPVLSGGNAVLLVTLDGDSDVPISPDATGRDAAVSI SGNAAAIPVSTPAVNDENKQPVTTSSPSRSFPVGKMQSDIRKFFASSQP TSCTNLQKSNVSSK TRKRALTSNSG SPV IKRRRV SKKAKKP+KTIPLVNGICSKCSRSLVCYSVNNSDNAKLVLSPKNNVKEPIKLSPENNYICIYCELL
Sbjct:   41 VSNTNTIKRTCSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKKKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATESEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLVSGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGFPMSSDGNPVPPVTHIQGDIGSHLISDGDAALPVLSGGNAVLLVTLDGDSDVPISPDATGRDAAVSIISGNAAAIPVSTPAVNDENKQPVTTSSPSRSFPVGKMQSDIRKFFASSQPTTSCTNLQKSNVSSKSTRKRALTSNSGPSPVSIKRRRVCSKKAKKPIKTIPLVNGICSKCSRSLVCYSVNNSDNAKLVLSPKNNVKEPIKLSPENNYICIYCELL 531
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Planmine SMEST
Match: SMESG000026497.1 (SMESG000026497.1)

HSP 1 Score: 920.613 bits (2378), Expect = 0.000e+0
Identity = 458/472 (97.03%), Postives = 461/472 (97.67%), Query Frame = 3 
Query:   57 ISNTNTIKRTCSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGFQMSPDGNPVLPVTHIQGDKGSHLISDGDAALPVLSGGNAVLLVTLDGDSDVPISPDATGRDAAVSITSGNAAAIPVSTPAVNDENKQPVTTSSPSRSFPVGKMQSDIRKFFASSQPITSCTNLQKSNVSSKLTRKRALTSNSGASPVFIXXXXXXXXXXXXPMKTIPLVNGICSKCSRSLVCYSVNNSDNAKLVLSPKNNVK 1472
            ISNTNTIKRTCSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKK+KAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATE+EVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCL SGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGF MS DGNPV PVTHIQGD GSHLISDGDAALPVLSGGNAVLLVTLDGDSDVPISPDATGRDAAVSI SGNAAAIPVSTPAVNDENKQPVTTSSPSRSFPVGKMQSDIRKFFASSQP TSCTNLQKSNVSSK TRKRALTSNSG SPV IKRRRV SKKAKKP+KTIPLVNGICSKCSRSLVCYSVNNSDNAKLVLSPKNNVK
Sbjct:   39 ISNTNTIKRTCSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKKKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATESEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLVSGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGFPMSSDGNPVPPVTHIQGDIGSHLISDGDAALPVLSGGNAVLLVTLDGDSDVPISPDATGRDAAVSIISGNAAAIPVSTPAVNDENKQPVTTSSPSRSFPVGKMQSDIRKFFASSQPTTSCTNLQKSNVSSKSTRKRALTSNSGPSPVSIKRRRVCSKKAKKPIKTIPLVNGICSKCSRSLVCYSVNNSDNAKLVLSPKNNVK 510
[Close]
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Planmine SMEST
Match: SMESG000026497.1 (SMESG000026497.1)

HSP 1 Score: 919.842 bits (2376), Expect = 0.000e+0
Identity = 457/472 (96.82%), Postives = 461/472 (97.67%), Query Frame = 3 
Query:   57 ISNTNTIKRTCSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGFQMSPDGNPVLPVTHIQGDKGSHLISDGDAALPVLSGGNAVLLVTLDGDSDVPISPDATGRDAAVSITSGNAAAIPVSTPAVNDENKQPVTTSSPSRSFPVGKMQSDIRKFFASSQPITSCTNLQKSNVSSKLTRKRALTSNSGASPVFIXXXXXXXXXXXXPMKTIPLVNGICSKCSRSLVCYSVNNSDNAKLVLSPKNNVK 1472
            +SNTNTIKRTCSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKK+KAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATE+EVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCL SGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGF MS DGNPV PVTHIQGD GSHLISDGDAALPVLSGGNAVLLVTLDGDSDVPISPDATGRDAAVSI SGNAAAIPVSTPAVNDENKQPVTTSSPSRSFPVGKMQSDIRKFFASSQP TSCTNLQKSNVSSK TRKRALTSNSG SPV IKRRRV SKKAKKP+KTIPLVNGICSKCSRSLVCYSVNNSDNAKLVLSPKNNVK
Sbjct:   41 VSNTNTIKRTCSLCDISCDEPYLGELLTESNFSIHENCLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKKKAGIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIESCGNIVPECAACLDSMAATESEVLASVPDNEWDLFMRNVATRSVMHAGCCTRAWLHRTCMAKHCLVSGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAWEMEPGAFQEMYEDAAPLNHDEAITISSDSDTGFPMSSDGNPVPPVTHIQGDIGSHLISDGDAALPVLSGGNAVLLVTLDGDSDVPISPDATGRDAAVSIISGNAAAIPVSTPAVNDENKQPVTTSSPSRSFPVGKMQSDIRKFFASSQPTTSCTNLQKSNVSSKSTRKRALTSNSGPSPVSIKRRRVCSKKAKKPIKTIPLVNGICSKCSRSLVCYSVNNSDNAKLVLSPKNNVK 512
The following BLAST results are available for this feature:
BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PHF74.395e-3534.48PHD finger protein 7 [Source:HGNC Symbol;Acc:HGNC:... [more]
PHF71.076e-3432.00PHD finger protein 7 [Source:HGNC Symbol;Acc:HGNC:... [more]
G2E34.496e-3330.57G2/M-phase specific E3 ubiquitin protein ligase [S... [more]
G2E32.320e-3232.42G2/M-phase specific E3 ubiquitin protein ligase [S... [more]
G2E32.935e-3232.42G2/M-phase specific E3 ubiquitin protein ligase [S... [more]
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BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Phf73.385e-3030.00gene:FBgn0031091 transcript:FBtr0070027[more]
Phf73.385e-3030.00gene:FBgn0031091 transcript:FBtr0333547[more]
Phf73.385e-3030.00gene:FBgn0031091 transcript:FBtr0333546[more]
pie4.321e-2227.50gene:FBgn0005683 transcript:FBtr0333490[more]
pie4.321e-2227.50gene:FBgn0005683 transcript:FBtr0080046[more]
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BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
g2e31.950e-2930.94G2/M-phase specific E3 ubiquitin protein ligase [S... [more]
phf111.312e-1632.26PHD finger protein 11 [Source:ZFIN;Acc:ZDB-GENE-08... [more]
phf114.561e-1632.26PHD finger protein 11 [Source:ZFIN;Acc:ZDB-GENE-08... [more]
phf118.912e-1632.26PHD finger protein 11 [Source:ZFIN;Acc:ZDB-GENE-08... [more]
phf64.643e-1133.88PHD finger protein 6 [Source:ZFIN;Acc:ZDB-GENE-030... [more]
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BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
G2E33.475e-3633.61G2/M-phase specific E3 ubiquitin protein ligase [S... [more]
dnajc82.643e-1131.71DnaJ heat shock protein family (Hsp40) member C8 [... [more]
ENSXETT00000027447.11.124e-928.46uncharacterized LOC100492657 [Source:NCBI gene;Acc... [more]
ENSXETT00000027460.11.412e-928.46uncharacterized LOC100492657 [Source:NCBI gene;Acc... [more]
tgif23.175e-934.96TGFB induced factor homeobox 2 [Source:Xenbase;Acc... [more]
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BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
G2e33.990e-3735.16G2/M-phase specific E3 ubiquitin ligase [Source:MG... [more]
G2e37.879e-3735.16G2/M-phase specific E3 ubiquitin ligase [Source:MG... [more]
G2e37.981e-3735.16G2/M-phase specific E3 ubiquitin ligase [Source:MG... [more]
Phf78.759e-3732.00PHD finger protein 7 [Source:MGI Symbol;Acc:MGI:19... [more]
Phf72.828e-2631.08PHD finger protein 7 [Source:MGI Symbol;Acc:MGI:19... [more]
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BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5RJY2|G2E3_MOUSE5.512e-3635.16G2/M phase-specific E3 ubiquitin-protein ligase OS... [more]
sp|Q9DAG9|PHF7_MOUSE6.127e-3632.00PHD finger protein 7 OS=Mus musculus OX=10090 GN=P... [more]
sp|Q9BWX1|PHF7_HUMAN5.169e-3432.00PHD finger protein 7 OS=Homo sapiens OX=9606 GN=PH... [more]
sp|Q6AXW4|PHF7_RAT6.571e-3433.91PHD finger protein 7 OS=Rattus norvegicus OX=10116... [more]
sp|Q5F4A1|G2E3_CHICK2.969e-3233.33G2/M phase-specific E3 ubiquitin-protein ligase OS... [more]
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BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A430QFT93.349e-3928.48G2/M phase-specific E3 ubiquitin-protein ligase OS... [more]
A0A210Q9Q54.520e-3933.47G2/M phase-specific E3 ubiquitin-protein ligase OS... [more]
A0A1S3J2F43.295e-3632.55uncharacterized protein LOC106169620 OS=Lingula un... [more]
G3W5T14.505e-3532.85PHD finger protein 7 OS=Sarcophilus harrisii OX=93... [more]
G3W5T06.328e-3532.85PHD finger protein 7 OS=Sarcophilus harrisii OX=93... [more]
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BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
g2e32.249e-2829.07G2/M-phase specific E3 ubiquitin protein ligase [S... [more]
ENSAMXT00000011361.22.703e-1433.90pep primary_assembly:Astyanax_mexicanus-2.0:19:256... [more]
ENSAMXT00000044632.12.703e-1433.90pep primary_assembly:Astyanax_mexicanus-2.0:19:256... [more]
phf63.339e-1133.61PHD finger protein 6 [Source:NCBI gene;Acc:1030262... [more]
phf64.879e-1036.00PHD finger protein 6 [Source:NCBI gene;Acc:1030262... [more]
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BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000007094.12.662e-2833.64pep scaffold:Pmarinus_7.0:GL483693:43:11634:1 gene... [more]
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BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO317692.326e-3029.27Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO308597.534e-826.98Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
g2e32.599e-3332.13G2/M-phase specific E3 ubiquitin protein ligase [S... [more]
phf63.815e-1032.03PHD finger protein 6 [Source:NCBI gene;Acc:1011609... [more]
phf64.498e-1032.03PHD finger protein 6 [Source:NCBI gene;Acc:1011609... [more]
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BLAST of G2/M phase-specific E3 ubiquitin-protein ligase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000026497.10.000e+096.95SMESG000026497.1[more]
SMESG000026497.10.000e+097.15SMESG000026497.1[more]
SMESG000026497.10.000e+096.95SMESG000026497.1[more]
SMESG000026497.10.000e+097.03SMESG000026497.1[more]
SMESG000026497.10.000e+096.82SMESG000026497.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027120 ID=SMED30027120|Name=G2/M phase-specific E3 ubiquitin-protein ligase|organism=Schmidtea mediterranea sexual|type=transcript|length=1569bp
GTTTTATATAAAATTGAATGGTTAAAAAAAGACGTAGTGTTAAAAAGGCG
TCTAAAATATCAAATACTAATACAATAAAACGCACATGTTCTCTCTGCGA
TATAAGTTGTGATGAGCCTTATCTAGGTGAATTACTGACCGAATCTAATT
TTTCGATTCATGAAAATTGCTTATTTTTCAGTCCAGTTTTACACCAGCAA
CTTGATCCATCTGTGAAAAGAAAAGGCTTCATTCTTGGATTTAGAATTTC
GGATATAGAAAAAGAAATCAAAAGAATAAGTCGTTTAAAATGTTTTGTGT
GCAAAAAAAGGAAGGCTGGGATTGGATGTTGCAATGAAATATGTAAGCGT
TCATATCACTTAGTATGTGGTCTGCAATCTAATGCAATGGGAATATTCAC
TGATTCGTTCACTTTCTACTGCAAAGAACACGCTATACGACAGGACTTAC
GGAAGCAAATCGAAAGCTGTGGCAATATTGTACCAGAATGCGCCGCCTGC
CTCGATTCGATGGCAGCAACTGAGAACGAGGTGCTTGCGTCAGTACCGGA
TAATGAATGGGATCTATTTATGCGTAATGTAGCCACCCGTAGTGTTATGC
ACGCAGGATGCTGTACGCGCGCATGGTTGCACAGGACATGCATGGCCAAA
CACTGCCTGGCGTCTGGCCTGCATCACGTCAAATGCCCGAATTGTGGTAA
TAAGTCCGTTTACATCGACACCGCTCTCAAATATGGTTTGTGGATTCCAG
ATAGGGATGCCGCATGGGAAATGGAGCCCGGTGCATTCCAGGAGATGTAC
GAGGATGCAGCGCCGCTGAATCATGATGAAGCTATTACAATTTCATCTGA
TAGTGATACAGGGTTTCAGATGTCGCCTGATGGTAATCCGGTCCTTCCAG
TGACACATATCCAGGGTGATAAAGGCTCTCATCTTATATCTGATGGTGAT
GCAGCCCTTCCGGTATTGTCAGGGGGTAACGCGGTTCTTCTAGTCACGCT
TGATGGTGATTCAGACGTTCCTATTTCACCTGATGCTACTGGTCGTGATG
CAGCTGTTTCGATTACATCTGGGAACGCTGCTGCTATTCCAGTATCAACA
CCAGCTGTCAATGATGAAAATAAACAACCAGTTACAACATCATCACCCAG
TAGATCCTTTCCAGTTGGGAAAATGCAGTCGGATATCCGGAAATTCTTTG
CTTCATCTCAGCCGATCACTTCATGTACTAATTTACAGAAATCGAACGTT
TCGTCTAAATTGACAAGGAAACGAGCATTGACATCAAATTCTGGCGCAAG
TCCAGTCTTCATCAAGCGACGGCGGGTAAGTTCTAAAAAAGCCAAGAAAC
CGATGAAAACCATACCACTTGTTAATGGTATTTGCTCAAAATGTTCACGC
TCTTTAGTTTGCTACAGCGTCAACAACAGTGACAATGCCAAATTGGTTCT
TTCGCCAAAGAATAATGTCAAGGAACCTATCAAGCTATCGCCTGAAAATA
ATTATATTTGCATTTATTGTGAACTGCTGTGAAGGCATTTGTTGTAATTT
AATTAAATTTTCAGATTTT
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protein sequence of SMED30027120-orf-1

>SMED30027120-orf-1 ID=SMED30027120-orf-1|Name=SMED30027120-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=505bp
MVKKRRSVKKASKISNTNTIKRTCSLCDISCDEPYLGELLTESNFSIHEN
CLFFSPVLHQQLDPSVKRKGFILGFRISDIEKEIKRISRLKCFVCKKRKA
GIGCCNEICKRSYHLVCGLQSNAMGIFTDSFTFYCKEHAIRQDLRKQIES
CGNIVPECAACLDSMAATENEVLASVPDNEWDLFMRNVATRSVMHAGCCT
RAWLHRTCMAKHCLASGLHHVKCPNCGNKSVYIDTALKYGLWIPDRDAAW
EMEPGAFQEMYEDAAPLNHDEAITISSDSDTGFQMSPDGNPVLPVTHIQG
DKGSHLISDGDAALPVLSGGNAVLLVTLDGDSDVPISPDATGRDAAVSIT
SGNAAAIPVSTPAVNDENKQPVTTSSPSRSFPVGKMQSDIRKFFASSQPI
TSCTNLQKSNVSSKLTRKRALTSNSGASPVFIKRRRVSSKKAKKPMKTIP
LVNGICSKCSRSLVCYSVNNSDNAKLVLSPKNNVKEPIKLSPENNYICIY
CELL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR013083Znf_RING/FYVE/PHD
IPR034732EPHD
Vocabulary: biological process
TermDefinition
GO:0000209protein polyubiquitination
GO:0001824blastocyst development
GO:0006915apoptotic process
GO:0007275multicellular organism development
GO:0016567protein ubiquitination
GO:2001243negative regulation of intrinsic apoptotic signaling pathway
Vocabulary: molecular function
TermDefinition
GO:0004842ubiquitin-protein transferase activity
GO:0008270zinc ion binding
GO:0016740transferase activity
GO:0046872metal ion binding
GO:0061630ubiquitin protein ligase activity
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
GO:0005730nucleolus
GO:0005737cytoplasm
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF13771zf-HC5HC2Hcoord: 47..137
e-value: 7.0E-14
score: 51.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 360..382
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 403..429
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 403..436
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 362..382
NoneNo IPR availablePANTHERPTHR12420PHD FINGER PROTEINcoord: 13..260
NoneNo IPR availablePANTHERPTHR12420:SF37G2/M PHASE-SPECIFIC E3 UBIQUITIN-PROTEIN LIGASEcoord: 13..260
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3DG3DSA:3.30.40.10coord: 17..140
e-value: 3.4E-22
score: 80.8
IPR034732Extended PHD (ePHD) domainPROSITEPS51805EPHDcoord: 20..138
score: 21.797