SMED30027074
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30027074 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 13Note: Hover over icons to view figure legend
Alignments
SMED30027074 aligns in the following genomic locations:
Homology
BLAST of SMED30027074 vs. Planmine SMEST
Match: SMESG000005752.1 (SMESG000005752.1) HSP 1 Score: 346.665 bits (888), Expect = 3.338e-109 Identity = 257/257 (100.00%), Postives = 257/257 (100.00%), Query Frame = 3 Query: 3 EAFKEVLNEVSLENKIEENVFQIKAIEKQPHFLEELVTENIAEEEIKTIDAIVVPSDAMVPVLSEAVEQTQKYEQGEINADVAEIEQVIASKSVVGKVIESRAIELESEKPKLNLLXXXXXXXXXXXXXXXXXXXXXXXXXXXDAENQELEADFKAKESLALQNVSENVENICDYAGHXXXXXXXXXXXXXXXXXXXYDTIAIVEHPQTLEAMXXXXXXXXXXXXXXXXXVKTEKKLSTLSEAVEQNVKIEQGGVNE 773 EAFKEVLNEVSLENKIEENVFQIKAIEKQPHFLEELVTENIAEEEIKTIDAIVVPSDAMVPVLSEAVEQTQKYEQGEINADVAEIEQVIASKSVVGKVIESRAIELESEKPKLNLLEESKIETEITEKEESIEDSFEIEIELSDAENQELEADFKAKESLALQNVSENVENICDYAGHLSEEELNEVYLENEVEDNVYDTIAIVEHPQTLEAMATENIIEQEINTIEATIVKTEKKLSTLSEAVEQNVKIEQGGVNE Sbjct: 8240 EAFKEVLNEVSLENKIEENVFQIKAIEKQPHFLEELVTENIAEEEIKTIDAIVVPSDAMVPVLSEAVEQTQKYEQGEINADVAEIEQVIASKSVVGKVIESRAIELESEKPKLNLLEESKIETEITEKEESIEDSFEIEIELSDAENQELEADFKAKESLALQNVSENVENICDYAGHLSEEELNEVYLENEVEDNVYDTIAIVEHPQTLEAMATENIIEQEINTIEATIVKTEKKLSTLSEAVEQNVKIEQGGVNE 8496 HSP 2 Score: 99.7525 bits (247), Expect = 1.911e-23 Identity = 114/249 (45.78%), Postives = 171/249 (68.67%), Query Frame = 3 Query: 21 LNEVSLENKIEENVFQIKAIEKQPHFLEELVTENIAEEEIKTIDAIVVPSDAMVPVLSEAVEQTQKYEQGEINADVAEIEQVIASKSVVGKVIESRAIELESEKPKLNLLXXXXXXXXXXXXXX---------XXXXXXXXXXXXXDAENQELEADFKAKESLALQNVSENVENICDYAGHXXXXXXXXXXXXXXXXXXXYDTIAIVEHPQTLEAMXXXXXXXXXXXXXXXXXVKTEKKLSTLSEAVEQ 740 LNEV LEN++E+NV+ AI + P LE + TENI E+EI TI+A +V ++ + LSEAVEQ K EQG +N D+ EI + AS +++ +E++ SEK +N+LE SK+E + ++ SIEDSFEIE+ELSD EN+++EADFK K++ LQNVSEN+E ++ ++ +E L EV +++++E+NV+ T I E P+ LEA+ ENI+E+E++ +E T+VKTEK+ + L+EAVEQ Sbjct: 8423 LNEVYLENEVEDNVYDTIAIVEHPQTLEAMATENIIEQEINTIEATIVKTEKKLSTLSEAVEQNVKIEQGGVNEDILEIIETRASTALIENSLENK-----SEKLDVNVLENSKVEETMKDEAVFEDIFVQEESIEDSFEIEMELSDLENEDVEADFKTKDNKELQNVSENLEITDEHEANIHDEALEEVSIQHKLEENVFKTEIIKEQPEFLEALVAENIVEEEMHNVEGTLVKTEKQQTVLAEAVEQ 8666 HSP 3 Score: 88.9669 bits (219), Expect = 8.916e-20 Identity = 100/251 (39.84%), Postives = 158/251 (62.95%), Query Frame = 3 Query: 9 FKEVLNEVSLENKIEENVFQIKAIEKQPHFLEELVTENIAEEEIKTIDAIVVPSDAMVPVL-SEAVEQTQKYEQGEINADVAEIEQVIASKSVVGKVIESRAIELESEKPKLNLLXXXXXXXXXXXXXXXXXXXXXXXXXXXDAENQELEADFKAKESLALQNVSENVENICDYAGHXXXXXXXXXXXXXXXXXXXYDTIAIVEHPQTLEAMXXXXXXXXXXXXXXXXXVKTEKKLSTLSEAVEQNVKIEQ 758 ++++L+ VSLENKIE +V + + I++Q FLE LVT+NI EEE+ +++ + V + + VL SE+VE K ++ EI D+AEI+Q ++ +VV +E + I+ +++KP++N+L E+ I++E+ + EN +EADFK K++ LQ VSENVE I D + +E ++V +T I E+PQ+LEA E+I+E+EINTIEATI+K + +L LSEAVEQ ++EQ Sbjct: 7191 YQDLLDVVSLENKIENDVLETRVIKEQSEFLEPLVTDNIPEEEMNSVELVTVKPEELTTVLISESVE---KVDESEI-IDIAEIKQTDSTTTVVENFLEIQVIDEKADKPEINILTETNIQSEVEMETALEH-----------TENVNVEADFKTKDNEELQIVSENVE-IVDEHETETCKEEFYEAPLENKMESVIETELIEEYPQSLEASNVEDIVEEEINTIEATIIKPKNELLALSEAVEQIERVEQ 7425 HSP 4 Score: 82.4185 bits (202), Expect = 1.417e-17 Identity = 106/268 (39.55%), Postives = 163/268 (60.82%), Query Frame = 3 Query: 9 FKEVLNEVSLENKIEENVFQIKAIEKQPHFLEELVTENIAEEEIKTIDAIVVPSDAMVPVLSEAVEQTQKYEQGEINADVAEIEQVIASKSVV-GKVIESR------------AIELESEKPKLNLLXXXXXXXXXXXXXXXXXXXXXXXXXXXDAENQELEADFKAKESLALQNVSENVENICDYAGHXXXXXXXXXXXXXXXXXXXYDTIAIVEHPQTLEAMXXXXXXXXXXXXXXXXXVKTEKKLSTLSEAVEQNVKIEQGGVNE 773 ++E L+EV LEN E NVF+ + I++ P FLE L + +A EE++ ++AI+V ++ VLSEAVEQ + EQ EI D+A IEQ I +V+ +V+E++ IE+E+ P+ E EKEESI+DSFEIE+ELS+ +N++++ +FK K+ + SEN+E + + S E ++EV L + VE N+++T I E PQ LE +I+E+E ++E TIVK E K+ LSEA+EQ ++Q +NE Sbjct: 7874 YEERLDEVQLENVTELNVFKTEIIQEHPQFLESLTNDTMAGEEMRILEAIIVAPKEVIKVLSEAVEQIETIEQSEIQDDLANIEQDIVLANVIEAQVLETKLEQADEYISMETKIEIETPNPE-------ATTEETIEKEESIDDSFEIEMELSEVKNEDVKEEFKMKDKVEHHGASENIEIVHEQKAETSGETMDEVCLASRVEQNLFETQVIEEIPQFLETTIISDIVEEETKSVEGTIVKPEVKMEILSEAIEQTQNLKQSEINE 8134 HSP 5 Score: 80.4925 bits (197), Expect = 7.084e-17 Identity = 75/167 (44.91%), Postives = 116/167 (69.46%), Query Frame = 3 Query: 15 EVLNEVSLENKIEENVFQIKAIEKQPHFLEELVTENIAEEEIKTIDAIVVPSDAMVPVLSEAVEQTQKYEQGEINADVAEIEQVIASKSVVGKVIESRAIELESEKPKLNLLXXXXXXXXXXXXXXXXXXXXXXXXXXXDAENQELEADFKAKESLALQNVSENVEN 515 E L EVS+++K+EENVF+ + I++QP FLE LV ENI EEE+ ++ +V ++ VL+EAVEQ ++ E + +V+EI+Q + S +V+ V+E+ E E ++ + ++ + E+EESIEDSFEIE+ELSD EN+++EA+FK K++ LQ +SENVEN Sbjct: 8602 EALEEVSIQHKLEENVFKTEIIKEQPEFLEALVAENIVEEEMHNVEGTLVKTEKQQTVLAEAVEQIKELEHDK--DEVSEIKQALVSSAVMEDVLETNIHESEVKELDIKVI-------DSIEREESIEDSFEIEMELSDVENEDVEAEFKTKDNKELQILSENVEN 8759 HSP 6 Score: 78.9518 bits (193), Expect = 2.209e-16 Identity = 114/262 (43.51%), Postives = 170/262 (64.89%), Query Frame = 3 Query: 3 EAFKEVLNEVSLENKIEENVFQIKAIEKQPHFLEELVTENIAEEEIKTIDAIVVPSDAMVPVLSEAVEQTQKYEQGEINADVAEIEQVIASKSVVGKVIESRAIELESEKPKLNLLXXXXXXXXXXXX----------XXXXXXXXXXXXXXXDAENQELEADFKAKESLALQNVSENVENICDYAGHXXXXXXXXXXXXXXXXXXXYDTIAIVEHPQTLEAMXXXXXXXXXXXXXXXXXVKTEKKLSTLSEAVEQNVKIEQ 758 E F+E +EV+LE+K EENVF+ + IE+ P F+E L+T+NI +E + V VLSE VEQTQK ++ E+ ++ EI+ + A S++ ++E + E +SE P++N+LEE K E E+ E +ESIE SFEIE+ELSD E + ++ADF+ K++ LQ +SEN E ++ +EEL EV LE+++E+N + I EHPQ LE ENI+E+EINT+EATIV++E++L LSE +EQ K+E+ Sbjct: 8766 ETFQE-FDEVNLESKAEENVFKTQVIEEHPQFVEALITDNIEDEVVND-----------VAVLSEGVEQTQKLDENEVKEEIPEIKPITALTSLIKNILEDKVCEHKSEMPEVNVLEEVKTEMEVVETEDVIKEIIEKQESIEGSFEIEMELSDIEREIVKADFETKDNKELQVLSENAEIQEEHETEKCKEELYEVNLESKMEENASMSKVIEEHPQILEKEINENIVEEEINTVEATIVRSEEQLPVLSEVIEQIEKLEK 9015 HSP 7 Score: 73.1738 bits (178), Expect = 1.968e-14 Identity = 107/265 (40.38%), Postives = 165/265 (62.26%), Query Frame = 3 Query: 3 EAFKEVLNEVSLENKIEENVFQIKAIEKQPHFLEELVTENIAEEEIKTIDAIVVPSDAMVPVLSEAVEQTQKYEQGEINADVAEIEQVIASKSVVGKVIESRAIELESEKPKLNLLXXXXXXXXXXXX---------XXXXXXXXXXXXXXXDAENQELEADFKAKESLALQNVSENVENICDYAGHXXXXXXXXXXXXXXXXXXXYDTIAIVEHPQTLEAMXXXXXXXXXXXXXXXXXVKTEKKLSTLSEAVEQNVKIEQGGVN 770 E E ++EV L +++E+N+F+ + IE+ P FLE + +I EEE K+++ +V + + +LSEA+EQTQ +Q EIN +++EI+ AS V+ E + L+ E + +LEES + E+TE ES+EDSFE+E+EL D E+ +EA+FK K+S + VS +E + ++ +E LNEV LEN++E+NV+ AI + P LE + TENI E+EI TI+A +V ++ + LSEAVEQ K EQG +N Sbjct: 8055 ETSGETMDEVCLASRVEQNLFETQVIEEIPQFLETTIISDIVEEETKSVEGTIVKPEVKMEILSEAIEQTQNLKQSEIN-EISEIKPNAASVLVIDNSPEENVLGLKPETFDVPILEESNKQDEVTEMEVKGNIVVNAESLEDSFEMEMELLDVESANVEAEFKTKDSEDFKIVSGCIETVDEHEAEAFKEVLNEVSLENKIEENVFQIKAIEKQPHFLEELVTENIAEEEIKTIDAIVVPSDAMVPVLSEAVEQTQKYEQGEIN 8318 HSP 8 Score: 72.4034 bits (176), Expect = 4.234e-14 Identity = 110/262 (41.98%), Postives = 154/262 (58.78%), Query Frame = 3 Query: 3 EAFKEVLNEVSLENKIEENVFQIKAIEKQPHFLEELVTENIAEEEIKTIDAIVVPSDAMVPVLSEAVEQTQKYEQGEINADVAEIEQVIASKSVVGKVIESRAIELESEKPKLNLLXXXXXXXXXXXXXXXXXXXXXXXXXXXDA----------ENQELEADFKAKESLALQNVSENVENICDYAGHXXXXXXXXXXXXXXXXXXXYDTIAIVEHPQTLEAMXXXXXXXXXXXXXXXXXVKTEKKLSTLSEAVEQNVKIEQ 758 E KE E LENK+E +V + + IE+ P LE E+I EEEI TI+A ++ + LSEAVEQ ++ EQ EI + EI+Q +V E + E +SEKP++N+ EE KIE EI + EE E++ E E + D+ E++ +EADFK K++ LQ VSENVE I D + EE +NV++T I ++PQ LEA E+I+E+EINTIEATI+K + +L LSEAVEQ ++EQ Sbjct: 7352 ETCKEEFYEAPLENKME-SVIETELIEEYPQSLEASNVEDIVEEEINTIEATIIKPKNELLALSEAVEQIERVEQDEIKDEDLEIKQEETFTAVFENNSEDKVFETKSEKPEINVFEEMKIEEEIVKIEEVFEETIEKEESIEDSFEIEIELSDVEHENVEADFKTKDNEELQIVSENVE-IVDEHEAKTCEEELYKKPLESKVENVFETKVIEQYPQALEASNVEDIVEEEINTIEATIIKPKNELLALSEAVEQIERVEQ 7611 HSP 9 Score: 67.0106 bits (162), Expect = 2.281e-12 Identity = 71/169 (42.01%), Postives = 101/169 (59.76%), Query Frame = 3 Query: 36 LENKIEENVFQIKAIEKQPHFLEELVTENIAEEEIKTIDAIVVPSDAMVPVLSEAVEQTQKYEQGEINADVAEIEQVIASKSVVGKVIESRAIELESEKPKLNLLXXXXXXXXXXXXXXXXXXXXXXXXXXXDA----------ENQELEADFKAKESLALQNVSENVE 512 LE+K+E NVF+ K IE+ P LE E+I EEEI TI+A ++ + LSEAVEQ ++ EQ EI + EI+Q +V E + E +SEKP++N+ EE KIE EI + EE E++ E E + D+ E++ +EADFK K++ LQ VSEN+E Sbjct: 7549 LESKVE-NVFETKVIEQYPQALEASNVEDIVEEEINTIEATIIKPKNELLALSEAVEQIERVEQDEIKDEDLEIKQEETFTAVFENNSEDKFFETKSEKPEINVFEEMKIEEEIVKIEEVFEETIEKEDSIEDSFEIEIELSDVEHENVEADFKTKDNEELQIVSENIE 7716 HSP 10 Score: 52.7582 bits (125), Expect = 1.484e-7 Identity = 79/213 (37.09%), Postives = 129/213 (60.56%), Query Frame = 3 Query: 144 TIDAIVVPSDAMVPVLSEAVEQTQKYEQGEINADVAEIEQVIASKSVVGKVIESRAIELESEKPKLNLLXXXXXXXXXXXXXXXXXXXXXXXXXXXDAENQELEADF-------KAKESLALQNVSENVENICDYAGHXXXXXXXXXXXXXXXXXXXYDTIAIVEHPQTLEAMXXXXXXXXXXXXXXXXXVKTEKKLSTLSEAVEQNVKIEQG 761 + +AI++ D M +LSE+VEQ +K EQ EI + AE+ Q A ++V+ V++ +A E ++ KP+ ++LE + ++ EI + E +E+S E + + L+ F K + ++ LQNVSE++EN+ +A ++ EE L+EV LEN E NV+ T I EHPQ LE++ + + +E+ +EA IV ++ + LSEAVEQ IEQ Sbjct: 7743 STEAIIIKPDDMKNILSESVEQIEKVEQCEIKDEFAEVMQDKAFEAVLENVLDVKATEPKTTKPERSILEIANVQVEIMKDESFMEESIE--------KEESLQVSFESENEYAKTESNVDLQNVSEDIENLEQHATNIYEERLDEVQLENVTELNVFKTEIIQEHPQFLESLTNDTMAGEEMRILEAIIVAPKEVIKVLSEAVEQIETIEQS 7947 HSP 11 Score: 50.0618 bits (118), Expect = 9.529e-7 Identity = 46/109 (42.20%), Postives = 68/109 (62.39%), Query Frame = 3 Query: 3 EAFKEVLNEVSLENKIEENVFQIKAIEKQPHFLEELVTENIAEEEIKTIDAIVVPSDAMVPVLSEAVEQTQKYEQGEINADVAEIEQVIASKSVVGKVIESRAIELESE 329 E KE L EV+LE+K+EEN K IE+ P LE+ + ENI EEEI T++A +V S+ +PVLSE +EQ +K E+ I E E+ A ++V + E++A E++ Sbjct: 8941 EKCKEELYEVNLESKMEENASMSKVIEEHPQILEKEINENIVEEEINTVEATIVRSEEQLPVLSEVIEQIEKLEKDVIKDIHLETEEDKAVLTMVDAMEETKAESFETK 9049 The following BLAST results are available for this feature:
BLAST of SMED30027074 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30027074 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30027074 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30027074 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30027074 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30027074 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30027074 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30027074 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30027074 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30027074 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30027074 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30027074 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30027074 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30027074 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30027074 ID=SMED30027074|Name=SMED30027074|organism=Schmidtea mediterranea sexual|type=transcript|length=773bpback to top protein sequence of SMED30027074-orf-1 >SMED30027074-orf-1 ID=SMED30027074-orf-1|Name=SMED30027074-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=257bp EAFKEVLNEVSLENKIEENVFQIKAIEKQPHFLEELVTENIAEEEIKTIDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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