ENTH/VHS

Overview
NameENTH/VHS
Smed IDSMED30027059
Length (bp)321
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of ENTH/VHS (SMED30027059) t-SNE clustered cells

Violin plots show distribution of expression levels for ENTH/VHS (SMED30027059) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of ENTH/VHS (SMED30027059) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for ENTH/VHS (SMED30027059) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30027059

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30027059h1SMcG0001671 SmedASXL_015941SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30027059h1SMcG0001671 SmedASXL_015941SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of ENTH/VHS vs. TrEMBL
Match: A4RSE8 (CID domain-containing protein (Fragment) OS=Ostreococcus lucimarinus (strain CCE9901) OX=436017 GN=OSTLU_36236 PE=4 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 1.821e-7
Identity = 29/75 (38.67%), Postives = 42/75 (56.00%), Query Frame = 2 
Query:   35 INEISILSGGFK-EDEKVCQRICEKIMDNIKIADNNTKLCLFYSMDSIVKNICQPFNKFFAESLVETFVDTFKSV 256
            I  ++IL+G     DE   + I E I  +I+ A   TKLC FY +DS+VKN+  PF + FA  L + F+  +  V
Sbjct:   24 ITSLTILAGEIAGNDEARAEAIAELIRGHIRTAPAKTKLCGFYLLDSVVKNLRGPFVRCFATGLSDLFLPAYAKV 98
[Close]
BLAST of ENTH/VHS vs. TrEMBL
Match: A0A1J7IJU8 (Uncharacterized protein OS=Coniochaeta ligniaria NRRL 30616 OX=1408157 GN=CONLIGDRAFT_438724 PE=4 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 1.118e-6
Identity = 32/86 (37.21%), Postives = 47/86 (54.65%), Query Frame = 2 
Query:   41 EISILSGGFKEDEKVCQRICEKIMDNIKIADNNTKLCLFYSMDSIVKNICQPFNKFFAESLVETFVDTFKSVSCNLLKRQSNEIFK 298
            EIS L+   +E+ +    I E + D+IK      KL   Y +DSIVKN+  P+  FF   L  TF+D + SV  N  +R+ +E+ K
Sbjct:   28 EISNLTVVARENTEHALAIAETLQDHIKKVPPQRKLPALYVLDSIVKNVGTPYTLFFGRKLYNTFMDAYASVD-NQTRRKMDEMLK 112
[Close]
BLAST of ENTH/VHS vs. TrEMBL
Match: A0A0B1P8Q3 (Putative mrna cleavage factor complex component pcf11 OS=Uncinula necator OX=52586 GN=EV44_g2371 PE=4 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.734e-6
Identity = 31/86 (36.05%), Postives = 49/86 (56.98%), Query Frame = 2 
Query:   41 EISILSGGFKEDEKVCQRICEKIMDNIKIADNNTKLCLFYSMDSIVKNICQPFNKFFAESLVETFVDTFKSVSCNLLKRQSNEIFK 298
            EIS L+   KE+ +    I E + ++IK    N KL  FY +DS+VKN+  P+  FF   L  TF++ +  V  N ++R+ +E+ K
Sbjct:   27 EISNLTVIAKENTEHALAISEALKNHIKTTSPNKKLPSFYVLDSVVKNVGTPYTLFFGRQLYATFMEAYALVDNN-VRRKMDEMLK 111
[Close]
BLAST of ENTH/VHS vs. TrEMBL
Match: N1JGU4 (mRNA cleavage factor complex component Pcf11 OS=Blumeria graminis f. sp. hordei (strain DH14) OX=546991 GN=BGHDH14_bgh06283 PE=4 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.539e-6
Identity = 31/86 (36.05%), Postives = 48/86 (55.81%), Query Frame = 2 
Query:   41 EISILSGGFKEDEKVCQRICEKIMDNIKIADNNTKLCLFYSMDSIVKNICQPFNKFFAESLVETFVDTFKSVSCNLLKRQSNEIFK 298
            EIS L+   KE+ +    I E + D+IK      KL  FY +DS+VKN+  P+  FF   L  TF++ +  V  N ++R+ +E+ K
Sbjct:   27 EISNLTVIAKENTEHALAISEALKDHIKTTSPQKKLPSFYVLDSVVKNVGTPYTLFFGRQLYTTFMEAYALVDNN-VRRKMDEMLK 111
[Close]
BLAST of ENTH/VHS vs. TrEMBL
Match: A0A383UYW9 (Uncharacterized protein OS=Blumeria graminis f. sp. hordei OX=62688 GN=BLGHR1_15725 PE=4 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.539e-6
Identity = 31/86 (36.05%), Postives = 48/86 (55.81%), Query Frame = 2 
Query:   41 EISILSGGFKEDEKVCQRICEKIMDNIKIADNNTKLCLFYSMDSIVKNICQPFNKFFAESLVETFVDTFKSVSCNLLKRQSNEIFK 298
            EIS L+   KE+ +    I E + D+IK      KL  FY +DS+VKN+  P+  FF   L  TF++ +  V  N ++R+ +E+ K
Sbjct:   27 EISNLTVIAKENTEHALAISEALKDHIKTTSPQKKLPSFYVLDSVVKNVGTPYTLFFGRQLYTTFMEAYALVDNN-VRRKMDEMLK 111
[Close]
BLAST of ENTH/VHS vs. Planmine SMEST
Match: SMESG000059890.1 (SMESG000059890.1)

HSP 1 Score: 149.828 bits (377), Expect = 3.298e-43
Identity = 72/73 (98.63%), Postives = 73/73 (100.00%), Query Frame = 2 
Query:   35 INEISILSGGFKEDEKVCQRICEKIMDNIKIADNNTKLCLFYSMDSIVKNICQPFNKFFAESLVETFVDTFKS 253
            INEISILSGGFKEDEKVCQRIC+KIMDNIKIADNNTKLCLFYSMDSIVKNICQPFNKFFAESLVETFVDTFKS
Sbjct:   23 INEISILSGGFKEDEKVCQRICDKIMDNIKIADNNTKLCLFYSMDSIVKNICQPFNKFFAESLVETFVDTFKS 95
[Close]
BLAST of ENTH/VHS vs. Planmine SMEST
Match: SMESG000059830.1 (SMESG000059830.1)

HSP 1 Score: 48.1358 bits (113), Expect = 1.394e-7
Identity = 27/91 (29.67%), Postives = 49/91 (53.85%), Query Frame = 2 
Query:   35 INEISILSGGFKEDEKVCQRICEKIMDNIKIADNNTKLCLFYSMDSIVKNICQPFNKFFAESLVETFVDTFKSVSCNLLKRQSNEIFKIKI 307
            IN +S+++   +++  + + I +  M  I       KL  FY +DSI+KN+  P+ + FA+ +++ F+ TF          QSN+  KI+I
Sbjct:   25 INTLSVMAMNARDNPSLSKEIAKLAMSQIFKVVPQLKLTNFYVVDSIIKNVGSPYKEMFADKVLDAFIHTF----------QSNKDHKIRI 105
[Close]
BLAST of ENTH/VHS vs. Planmine SMEST
Match: SMESG000059830.1 (SMESG000059830.1)

HSP 1 Score: 47.3654 bits (111), Expect = 2.695e-7
Identity = 27/91 (29.67%), Postives = 49/91 (53.85%), Query Frame = 2 
Query:   35 INEISILSGGFKEDEKVCQRICEKIMDNIKIADNNTKLCLFYSMDSIVKNICQPFNKFFAESLVETFVDTFKSVSCNLLKRQSNEIFKIKI 307
            IN +S+++   +++  + + I +  M  I       KL  FY +DSI+KN+  P+ + FA+ +++ F+ TF          QSN+  KI+I
Sbjct:   25 INTLSVMAMNARDNPSLSKEIAKLAMSQIFKVVPQLKLTNFYVVDSIIKNVGSPYKEMFADKVLDAFIHTF----------QSNKDHKIRI 105
The following BLAST results are available for this feature:
BLAST of ENTH/VHS vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ENTH/VHS vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ENTH/VHS vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ENTH/VHS vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ENTH/VHS vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ENTH/VHS vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ENTH/VHS vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ENTH/VHS vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A4RSE81.821e-738.67CID domain-containing protein (Fragment) OS=Ostreo... [more]
A0A1J7IJU81.118e-637.21Uncharacterized protein OS=Coniochaeta ligniaria N... [more]
A0A0B1P8Q31.734e-636.05Putative mrna cleavage factor complex component pc... [more]
N1JGU42.539e-636.05mRNA cleavage factor complex component Pcf11 OS=Bl... [more]
A0A383UYW92.539e-636.05Uncharacterized protein OS=Blumeria graminis f. sp... [more]
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BLAST of ENTH/VHS vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ENTH/VHS vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ENTH/VHS vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ENTH/VHS vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ENTH/VHS vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ENTH/VHS vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000059890.13.298e-4398.63SMESG000059890.1[more]
SMESG000059830.11.394e-729.67SMESG000059830.1[more]
SMESG000059830.12.695e-729.67SMESG000059830.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027059 ID=SMED30027059|Name=ENTH/VHS|organism=Schmidtea mediterranea sexual|type=transcript|length=321bp
GTATCAACAAGCTGAGAGATACAGATAAAAATAAATAAATGAAATTTCCA
TTCTTTCTGGAGGATTTAAAGAAGATGAAAAAGTTTGTCAACGAATATGT
GAAAAAATTATGGATAATATAAAAATTGCAGATAACAACACAAAGCTATG
CCTATTTTATTCTATGGATTCTATTGTAAAAAACATATGTCAACCTTTTA
ATAAATTCTTTGCTGAGAGTCTCGTTGAAACTTTTGTCGATACTTTTAAA
TCTGTAAGTTGTAACTTATTGAAAAGACAAAGCAATGAAATTTTTAAAAT
AAAAATTGATTTTCTTTTTGA
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0046872metal ion binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR008942ENTH_VHS
IPR006569CID_dom