SMED30027044

Overview
NameSMED30027044
Smed IDSMED30027044
Length (bp)475
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30027044 (SMED30027044) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30027044 (SMED30027044) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30027044 (SMED30027044) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30027044 (SMED30027044) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30027044

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30027044h1SMcG0014491 Contig631newmark_estsPMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30027044h1SMcG0014491 Contig631newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30027044h1SMcG0021147 Contig631newmark_estsPMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30027044h1SMcG0021147 Contig631newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
epidermisSMED30027044h1SMcG0021147 dd_Smed_v4_2634_0_2dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30027044h1SMcG0021147 dd_Smed_v4_2634_0_2dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30027044h1SMcG0021147 OX_Smed_1.0.13802ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
testisSMED30027044h1SMcG0008861 Contig632uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30027044h1SMcG0008861 Contig632newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30027044h1SMcG0021147 Contig632uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30027044h1SMcG0021147 Contig632newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Alignments
SMED30027044 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000370:9836..10353 -2227
Homology
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BLAST of SMED30027044 vs. Planmine SMEST
Match: SMESG000024306.1 (SMESG000024306.1)

HSP 1 Score: 254.603 bits (649), Expect = 2.540e-88
Identity = 134/142 (94.37%), Postives = 137/142 (96.48%), Query Frame = 3 
Query:   27 MNENSFFNEKYKNLHYKSYENLDNNNFSSKRIILGVAIYSMSEICMRTVYSCICYKFPQMGKLIRRSWIYNHHSVQEYIIFLNNKLFKKTVDXXXXXXXXXXLHESSVLRAYLMPVAYPAKIYLGYSLSGYLCNDFYWCLFL 452
            MNENSFFNEKYKNLHYKSYENLDNNNFSSKRIILGVAIYSMSEIC+RTVYSCICYKFPQMGKLIRRSWIYNHHSVQEYIIFLN+K FKKTVDMN HMMKMNNLHESSVLRAYLMPVAYPAKIYLGYSLS Y+CND YW LFL
Sbjct:    1 MNENSFFNEKYKNLHYKSYENLDNNNFSSKRIILGVAIYSMSEICLRTVYSCICYKFPQMGKLIRRSWIYNHHSVQEYIIFLNHKRFKKTVDMNTHMMKMNNLHESSVLRAYLMPVAYPAKIYLGYSLSEYVCNDIYWRLFL 142
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BLAST of SMED30027044 vs. Planmine SMEST
Match: SMESG000024298.1 (SMESG000024298.1)

HSP 1 Score: 124.79 bits (312), Expect = 2.765e-37
Identity = 70/136 (51.47%), Postives = 88/136 (64.71%), Query Frame = 3 
Query:   27 MNENSFFNEKYKNLHYKSYENLDNNNFSSKRIILGVAIYSMSEICMRTVYSCICYKFPQMGKLIRRSWIYNHHSVQEYIIFLNNKLFKKTVDXXXXXXXXXXLHESSVLRAYLMPVAYPAKIYLGYSLSGYLCNDF 434
            MN+NS  NEKYKNLHYKSYENLDNNNFSSKRIILG+ IY +S ICM      + +K P +G  IRRS +Y+     + +  ++  L    V+    +  +N + +  VLR Y  PV +PAKIY  YSLSGYLCN F
Sbjct:    1 MNKNSINNEKYKNLHYKSYENLDNNNFSSKRIILGILIYPLSGICMSAGLMFMLFKKPIIGNYIRRSSLYHWLEAPKLVKSIDGILSILYVNPTIRLNLVNFVMDFMVLRIYFTPVIFPAKIYSAYSLSGYLCNKF 136
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BLAST of SMED30027044 vs. Planmine SMEST
Match: SMESG000024299.1 (SMESG000024299.1)

HSP 1 Score: 122.094 bits (305), Expect = 3.817e-36
Identity = 69/136 (50.74%), Postives = 81/136 (59.56%), Query Frame = 3 
Query:   27 MNENSFFNEKYKNLHYKSYENLDNNNFSSKRIILGVAIYSMSEICMRTVYSCICYKFPQMGKLIRRSWIYNHHSVQEYIIFLNNKLFKKTVDXXXXXXXXXXLHESSVLRAYLMPVAYPAKIYLGYSLSGYLCNDF 434
            MN+NS  NEKYKNLHYKSYENLDNNNFSSKRIILG+ IY +S ICM      +  K P +G  IRRS +Y      + + +    L     D       +    +  VLR Y  PV +PAKIY  YSLSGYLCN F
Sbjct:    1 MNKNSINNEKYKNLHYKSYENLDNNNFSSKRIILGILIYPLSGICMSAGLIFMLSKKPIIGNYIRRSSLYRWLEAPKLVKYGEGFLSTLISDPKERFYLLIAAADFIVLRIYFTPVIFPAKIYSAYSLSGYLCNKF 136
[Close]
BLAST of SMED30027044 vs. Planmine SMEST
Match: SMESG000024333.1 (SMESG000024333.1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.254e-20
Identity = 52/136 (38.24%), Postives = 72/136 (52.94%), Query Frame = 3 
Query:   27 MNENSFFNEKYKNLHYKSYENLDNNNFSSKRIILGVAIYSMSEICMRTVYSCICYKFPQMGKLIRRSWIYNHHSVQEYIIFLNNKLFKKTVDXXXXXXXXXXL---HESSVLRAYLMPVAYPAKIYLGYSLSGYLC 425
            MN++S  NEKY+ L  K  E +D  N S KR+I+   I+ +S +C+ T    +CYK   +GK+I+    YN+ S Q+ I     K+ +  +           L    ES VLR  L+PV  PAKIY  Y LSGY C
Sbjct:    1 MNKDSNTNEKYQKLLQKFNEYVDFKNLSIKRVIVAGVIHELSGLCLLTGLWYMCYKIRPVGKVIKSLPFYNNASHQKDISLAKQKINQMAIISWFSKTHKRMLISGTESFVLRKLLLPVTIPAKIYFAYHLSGYFC 136
The following BLAST results are available for this feature:
BLAST of SMED30027044 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027044 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027044 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027044 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027044 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027044 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027044 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027044 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027044 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027044 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027044 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027044 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027044 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30027044 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000024306.12.540e-8894.37SMESG000024306.1[more]
SMESG000024298.12.765e-3751.47SMESG000024298.1[more]
SMESG000024299.13.817e-3650.74SMESG000024299.1[more]
SMESG000024333.12.254e-2038.24SMESG000024333.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027044 ID=SMED30027044|Name=SMED30027044|organism=Schmidtea mediterranea sexual|type=transcript|length=475bp
GAAAAATTTTGTTTTTTCAAAATCAAATGAATGAAAACAGCTTTTTTAAT
GAAAAATATAAGAATTTGCATTATAAATCTTATGAAAACTTAGACAATAA
TAATTTTTCATCAAAGCGTATCATTCTTGGTGTTGCAATTTATTCCATGT
CTGAGATTTGCATGAGGACTGTATATTCGTGCATATGTTACAAATTTCCA
CAAATGGGTAAATTAATTAGACGTTCATGGATTTATAATCATCACAGCGT
TCAGGAATATATTATATTTCTCAATAACAAACTATTTAAGAAGACAGTAG
ATATGAATAATCATATGATGAAAATGAACAATCTCCATGAAAGTTCTGTT
TTGCGTGCATATCTTATGCCGGTAGCATATCCTGCTAAGATTTACTTAGG
ATATAGTTTAAGTGGATATTTATGTAATGATTTTTATTGGTGTTTGTTTC
TATAAAATATATGATATAAAATGGA
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protein sequence of SMED30027044-orf-1

>SMED30027044-orf-1 ID=SMED30027044-orf-1|Name=SMED30027044-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=151bp
KILFFQNQMNENSFFNEKYKNLHYKSYENLDNNNFSSKRIILGVAIYSMS
EICMRTVYSCICYKFPQMGKLIRRSWIYNHHSVQEYIIFLNNKLFKKTVD
MNNHMMKMNNLHESSVLRAYLMPVAYPAKIYLGYSLSGYLCNDFYWCLFL
*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
PLANA:0000418head