Innexin

Overview
NameInnexin
Smed IDSMED30027019
Length (bp)1593
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Innexin (SMED30027019) t-SNE clustered cells

Violin plots show distribution of expression levels for Innexin (SMED30027019) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Innexin (SMED30027019) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Innexin (SMED30027019) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30027019

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30027019h1SMcG0007095 SmedASXL_017882SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Innexin vs. Ensembl Celegans
Match: inx-1 (Innexin [Source:UniProtKB/TrEMBL;Acc:Q17394])

HSP 1 Score: 150.599 bits (379), Expect = 5.379e-40
Identity = 100/304 (32.89%), Postives = 172/304 (56.58%), Query Frame = 2 
Query:  437 YYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNG-ETKQESIFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKE---KYAIKLFDRG 1336
            YYQW+PF+L L+A+  +IP ++W+ L   ++G N+ +L   A  A + D ++R   V+ +A  +E  L EI+ + +  S     +C +        KR   Y+ + Y FIK+LYL N + Q+F++   L    +DN L +G  +L +  NG E +    FP+   C  ++R +G V+    QC L  N+ NEK+F+FLWFW  +V+ ++++S+  WI  S+L      F++KYL+  DL       +  +KKF  +FL   G+F +RMI+ ++GD++  +++  LW+ F +   K  I++F+ G
Sbjct:   99 YYQWVPFVLALEALCFYIPCIMWRGLLHWHSGINVQSLTQMACDARMMDADARAATVQTIAGHMEDAL-EIQREVTDVS----GMCVQ--------KRWANYVTLLYVFIKMLYLGNVVLQVFMLNSFL---GTDN-LFYGFHILRDLLNGREWEVSGNFPRVTMCDFEVRVLGNVHHHTVQCVLMINMFNEKIFLFLWFWYFMVAFVSAVSMFHWIIISFLPGQHMKFIRKYLRATDLA----TDRQSVKKFVHKFLGYDGVFCMRMISAHAGDILATELIVALWHNFNDRVRKSPIEMFEGG 381
[Close]
BLAST of Innexin vs. Ensembl Celegans
Match: unc-7 (Innexin unc-7 [Source:UniProtKB/Swiss-Prot;Acc:Q03412])

HSP 1 Score: 150.599 bits (379), Expect = 9.190e-40
Identity = 95/290 (32.76%), Postives = 165/290 (56.90%), Query Frame = 2 
Query:  437 YYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNG-ETKQESIFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNL-LKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIF 1300
            YYQW+PFIL ++A+  ++P +LW+ L   ++G N+  LV  A  A + D E + + V  +A+ ++  L  I     + S F  NL  ++   C  G+  G Y+ + Y  IK+LY  N + Q FL+  LL      N L +G  LL +  +  E +Q  +FP+   C  ++R +G ++    QC L  N+ NEK+F+FLWFW +   I+T  + + WI   ++     +F++KYL++   +  K +  ++ L+KF   FL+  G+F+LRMI+T++G+L++++++  LW  F
Sbjct:  160 YYQWVPFILAIEALLFYVPCILWRGLLYWHSGINLQGLVQMACDARLMDSEIKTRTVYTMARHMQDELTNIDRQGHSRSCFS-NL--QLGANC--GRHCGCYVTMLYIGIKVLYSANVLLQFFLLNHLL----GSNDLAYGFSLLKDLMHAIEWEQTGMFPRVTLCDFEVRVLGNIHRHTVQCVLMINMFNEKIFLFLWFWFLTCGIVTVCNTMYWILIMFIPSQGMSFVRKYLRVLPDHPAKPIADDVTLRKFTNNFLRKDGVFMLRMISTHAGELMSSELILALWQDF 440
[Close]
BLAST of Innexin vs. Ensembl Celegans
Match: inx-1 (Innexin [Source:UniProtKB/TrEMBL;Acc:Q17394])

HSP 1 Score: 148.673 bits (374), Expect = 9.771e-40
Identity = 96/292 (32.88%), Postives = 166/292 (56.85%), Query Frame = 2 
Query:  437 YYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNG-ETKQESIFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEK 1309
            YYQW+PF+L L+A+  +IP ++W+ L   ++G N+ +L   A  A + D ++R   V+ +A  +E  L EI+ + +  S     +C +        KR   Y+ + Y FIK+LYL N + Q+F++   L    +DN L +G  +L +  NG E +    FP+   C  ++R +G V+    QC L  N+ NEK+F+FLWFW  +V+ ++++S+  WI  S+L      F++KYL+  DL       +  +KKF  +FL   G+F +RMI+ ++GD++  +++  LW+ F ++
Sbjct:   99 YYQWVPFVLALEALCFYIPCIMWRGLLHWHSGINVQSLTQMACDARMMDADARAATVQTIAGHMEDAL-EIQREVTDVSG----MCVQ--------KRWANYVTLLYVFIKMLYLGNVVLQVFMLNSFL---GTDN-LFYGFHILRDLLNGREWEVSGNFPRVTMCDFEVRVLGNVHHHTVQCVLMINMFNEKIFLFLWFWYFMVAFVSAVSMFHWIIISFLPGQHMKFIRKYLRATDLA----TDRQSVKKFVHKFLGYDGVFCMRMISAHAGDILATELIVALWHNFNDR 369
[Close]
BLAST of Innexin vs. Ensembl Celegans
Match: inx-1 (Innexin [Source:UniProtKB/TrEMBL;Acc:Q17394])

HSP 1 Score: 148.673 bits (374), Expect = 9.771e-40
Identity = 96/292 (32.88%), Postives = 166/292 (56.85%), Query Frame = 2 
Query:  437 YYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNG-ETKQESIFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEK 1309
            YYQW+PF+L L+A+  +IP ++W+ L   ++G N+ +L   A  A + D ++R   V+ +A  +E  L EI+ + +  S     +C +        KR   Y+ + Y FIK+LYL N + Q+F++   L    +DN L +G  +L +  NG E +    FP+   C  ++R +G V+    QC L  N+ NEK+F+FLWFW  +V+ ++++S+  WI  S+L      F++KYL+  DL       +  +KKF  +FL   G+F +RMI+ ++GD++  +++  LW+ F ++
Sbjct:   99 YYQWVPFVLALEALCFYIPCIMWRGLLHWHSGINVQSLTQMACDARMMDADARAATVQTIAGHMEDAL-EIQREVTDVSG----MCVQ--------KRWANYVTLLYVFIKMLYLGNVVLQVFMLNSFL---GTDN-LFYGFHILRDLLNGREWEVSGNFPRVTMCDFEVRVLGNVHHHTVQCVLMINMFNEKIFLFLWFWYFMVAFVSAVSMFHWIIISFLPGQHMKFIRKYLRATDLA----TDRQSVKKFVHKFLGYDGVFCMRMISAHAGDILATELIVALWHNFNDR 369
[Close]
BLAST of Innexin vs. Ensembl Celegans
Match: unc-7 (Innexin unc-7 [Source:UniProtKB/Swiss-Prot;Acc:Q03412])

HSP 1 Score: 150.599 bits (379), Expect = 1.059e-39
Identity = 95/290 (32.76%), Postives = 165/290 (56.90%), Query Frame = 2 
Query:  437 YYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNG-ETKQESIFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNL-LKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIF 1300
            YYQW+PFIL ++A+  ++P +LW+ L   ++G N+  LV  A  A + D E + + V  +A+ ++  L  I     + S F  NL  ++   C  G+  G Y+ + Y  IK+LY  N + Q FL+  LL      N L +G  LL +  +  E +Q  +FP+   C  ++R +G ++    QC L  N+ NEK+F+FLWFW +   I+T  + + WI   ++     +F++KYL++   +  K +  ++ L+KF   FL+  G+F+LRMI+T++G+L++++++  LW  F
Sbjct:  173 YYQWVPFILAIEALLFYVPCILWRGLLYWHSGINLQGLVQMACDARLMDSEIKTRTVYTMARHMQDELTNIDRQGHSRSCFS-NL--QLGANC--GRHCGCYVTMLYIGIKVLYSANVLLQFFLLNHLL----GSNDLAYGFSLLKDLMHAIEWEQTGMFPRVTLCDFEVRVLGNIHRHTVQCVLMINMFNEKIFLFLWFWFLTCGIVTVCNTMYWILIMFIPSQGMSFVRKYLRVLPDHPAKPIADDVTLRKFTNNFLRKDGVFMLRMISTHAGELMSSELILALWQDF 453
[Close]
BLAST of Innexin vs. Ensembl Fly
Match: shakB (gene:FBgn0085387 transcript:FBtr0114645)

HSP 1 Score: 49.2914 bits (116), Expect = 4.329e-6
Identity = 55/208 (26.44%), Postives = 80/208 (38.46%), Query Frame = 2 
Query:  437 YYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNGETKQES-------IFPKFGFC-FVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFW 1036
            YYQW+ F L  QAI  + P  LWK    +  G  I  L+      +  + E + K+   L  + E + +              N     Y  C         L+     I  ++L+N                    + FG+K++      ET QE        IFP+   C F +    G V    + C LP NV+NEK++IFLWFW
Sbjct:   29 YYQWVAFTLFFQAILFYTPRWLWK----SWEGGKIHALIMDLDIGICSEAEKKQKKKLLLDYLWENLRYH-------------NWWAYRYYVC--------ELLALINVIGQMFLMNRFF-------------DGEFITFGLKVIDYM---ETDQEDRMDPMIYIFPRMTKCTFFKYGSSGEVEKHDAICILPLNVVNEKIYIFLWFW 195
[Close]
BLAST of Innexin vs. Ensembl Fly
Match: shakB (gene:FBgn0085387 transcript:FBtr0309369)

HSP 1 Score: 48.9062 bits (115), Expect = 7.997e-6
Identity = 55/208 (26.44%), Postives = 80/208 (38.46%), Query Frame = 2 
Query:  437 YYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNGETKQES-------IFPKFGFC-FVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFW 1036
            YYQW+ F L  QAI  + P  LWK    +  G  I  L+      +  + E + K+   L  + E + +              N     Y  C L   +          I  ++L+N                    + FG+K++      ET QE        IFP+   C F +    G V    + C LP NV+NEK++IFLWFW
Sbjct:  114 YYQWVAFTLFFQAILFYTPRWLWK----SWEGGKIHALIMDLDIGICSEAEKKQKKKLLLDYLWENLRYH-------------NWWAYRYYVCELLALINV--------IGQMFLMNRFF-------------DGEFITFGLKVIDYM---ETDQEDRMDPMIYIFPRMTKCTFFKYGSSGEVEKHDAICILPLNVVNEKIYIFLWFW 280
[Close]
BLAST of Innexin vs. Ensembl Fly
Match: shakB (gene:FBgn0085387 transcript:FBtr0309370)

HSP 1 Score: 48.9062 bits (115), Expect = 7.997e-6
Identity = 55/208 (26.44%), Postives = 80/208 (38.46%), Query Frame = 2 
Query:  437 YYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNGETKQES-------IFPKFGFC-FVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFW 1036
            YYQW+ F L  QAI  + P  LWK    +  G  I  L+      +  + E + K+   L  + E + +              N     Y  C L   +          I  ++L+N                    + FG+K++      ET QE        IFP+   C F +    G V    + C LP NV+NEK++IFLWFW
Sbjct:  114 YYQWVAFTLFFQAILFYTPRWLWK----SWEGGKIHALIMDLDIGICSEAEKKQKKKLLLDYLWENLRYH-------------NWWAYRYYVCELLALINV--------IGQMFLMNRFF-------------DGEFITFGLKVIDYM---ETDQEDRMDPMIYIFPRMTKCTFFKYGSSGEVEKHDAICILPLNVVNEKIYIFLWFW 280
[Close]
BLAST of Innexin vs. Ensembl Fly
Match: shakB (gene:FBgn0085387 transcript:FBtr0342859)

HSP 1 Score: 48.9062 bits (115), Expect = 7.997e-6
Identity = 55/208 (26.44%), Postives = 80/208 (38.46%), Query Frame = 2 
Query:  437 YYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNGETKQES-------IFPKFGFC-FVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFW 1036
            YYQW+ F L  QAI  + P  LWK    +  G  I  L+      +  + E + K+   L  + E + +              N     Y  C L   +          I  ++L+N                    + FG+K++      ET QE        IFP+   C F +    G V    + C LP NV+NEK++IFLWFW
Sbjct:  114 YYQWVAFTLFFQAILFYTPRWLWK----SWEGGKIHALIMDLDIGICSEAEKKQKKKLLLDYLWENLRYH-------------NWWAYRYYVCELLALINV--------IGQMFLMNRFF-------------DGEFITFGLKVIDYM---ETDQEDRMDPMIYIFPRMTKCTFFKYGSSGEVEKHDAICILPLNVVNEKIYIFLWFW 280
[Close]
BLAST of Innexin vs. Ensembl Fly
Match: shakB (gene:FBgn0085387 transcript:FBtr0112570)

HSP 1 Score: 48.9062 bits (115), Expect = 8.022e-6
Identity = 55/208 (26.44%), Postives = 80/208 (38.46%), Query Frame = 2 
Query:  437 YYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNGETKQES-------IFPKFGFC-FVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFW 1036
            YYQW+ F L  QAI  + P  LWK    +  G  I  L+      +  + E + K+   L  + E + +              N     Y  C L   +          I  ++L+N                    + FG+K++      ET QE        IFP+   C F +    G V    + C LP NV+NEK++IFLWFW
Sbjct:   98 YYQWVAFTLFFQAILFYTPRWLWK----SWEGGKIHALIMDLDIGICSEAEKKQKKKLLLDYLWENLRYH-------------NWWAYRYYVCELLALINV--------IGQMFLMNRFF-------------DGEFITFGLKVIDYM---ETDQEDRMDPMIYIFPRMTKCTFFKYGSSGEVEKHDAICILPLNVVNEKIYIFLWFW 264
[Close]
BLAST of Innexin vs. UniProt/SwissProt
Match: sp|Q03412|UNC7_CAEEL (Innexin unc-7 OS=Caenorhabditis elegans OX=6239 GN=unc-7 PE=1 SV=1)

HSP 1 Score: 147.902 bits (372), Expect = 2.565e-37
Identity = 91/290 (31.38%), Postives = 162/290 (55.86%), Query Frame = 2 
Query:  437 YYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNG-ETKQESIFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNL-LKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIF 1300
            YYQW+PFIL ++A+  ++P +LW+ L   ++G N+  LV  A  A + D E + + V  +A+ ++    E++  N       R+    +      G+  G Y+ + Y  IK+LY  N + Q FL+  LL      N L +G  LL +  +  E +Q  +FP+   C  ++R +G ++    QC L  N+ NEK+F+FLWFW +   I+T  + + WI   ++     +F++KYL++   +  K +  ++ L+KF   FL+  G+F+LRMI+T++G+L++++++  LW  F
Sbjct:  219 YYQWVPFILAIEALLFYVPCILWRGLLYWHSGINLQGLVQMACDARLMDSEIKTRTVYTMARHMQD---EVQLTNIDRQGHSRSCFSNLQLGANCGRHCGCYVTMLYIGIKVLYSANVLLQFFLLNHLL----GSNDLAYGFSLLKDLMHAIEWEQTGMFPRVTLCDFEVRVLGNIHRHTVQCVLMINMFNEKIFLFLWFWFLTCGIVTVCNTMYWILIMFIPSQGMSFVRKYLRVLPDHPAKPIADDVTLRKFTNNFLRKDGVFMLRMISTHAGELMSSELILALWQDF 501
[Close]
BLAST of Innexin vs. UniProt/SwissProt
Match: sp|O01393|UNC9_CAEEL (Innexin unc-9 OS=Caenorhabditis elegans OX=6239 GN=unc-9 PE=1 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 5.955e-34
Identity = 95/299 (31.77%), Postives = 162/299 (54.18%), Query Frame = 2 
Query:  437 YYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNGETKQES-IFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEKYAIKLFD 1330
            YYQW+PF+L L+A+  ++P ++W+LL   + G ++ +LV  A  + + D ESR + ++ +A  +E  L       S N         K+    I  +  GA +   Y  +KILY +N + Q+FL+   L   S      +G+++L++  NG   +ES  FP+   C  +++ +G V+    QC L  N+ NEK+F+FLWFW  +++  T  S+  WI+ S +   +  F+ KYL    +  YK V    L++F   FL+  G+F+LRM+AT++G+L   ++   LWN + +    K+ D
Sbjct:  101 YYQWVPFVLALEALLFYVPTIVWRLLSWQS-GIHVQSLVQMACDSRLLDLESRNRALQTIATNVEEALHVKHQVMSGNR-------LKLLNLIICTRSSGAAVTFLYISVKILYTVNIVGQIFLLNTFLGNRSK----WYGLQVLNDLMNGREWEESGHFPRVTLCDFEVKVLGNVHRHTVQCVLMINMFNEKIFLFLWFWYFLLAGATLCSLFYWIYISVVPSRQLNFVGKYL--TGIEGYKMVDSQSLRRFVFHFLRQDGVFLLRMVATHAGELPCYELAKTLWNNYCDNKEGKMHD 385
[Close]
BLAST of Innexin vs. UniProt/SwissProt
Match: sp|A8WVX4|INX19_CAEBR (Innexin-19 OS=Caenorhabditis briggsae OX=6238 GN=inx-19 PE=3 SV=3)

HSP 1 Score: 131.339 bits (329), Expect = 6.246e-32
Identity = 98/311 (31.51%), Postives = 161/311 (51.77%), Query Frame = 2 
Query:  437 YYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRL-GAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNGETKQES-IFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQK------------------NLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEK 1309
            YYQW+PFIL  +A+   +P + W+L    + G NI  L++ A  A        L +     K +EA+     D+    S  GR   R         + L G  + + Y F K+LY +N ++Q F++   L    S   + FG ++LS+ + G    E+  FP+   C  ++R++  +N    QCAL  N+INEK+F FLW W +I++IIT+ S + WI  S++   K  ++ K+++I + ++YK +QK                  +LL  F   FLKS G+ +LRMI+ ++GD+I   +V  LW  ++E+
Sbjct:  101 YYQWVPFILIAEALMFSLPCIFWRLCSFQS-GLNIQTLINAACDA------QALLDYSDRQKAVEAITCNFVDNLDLQSPNGRIRARGWIARIKFSRFLSGQCISIVYSFTKLLYSVNVVAQFFILNACL---KSSEFVFFGFQVLSDIWAGRPWTETGHFPRVTLCDFEVRYLANLNRYTVQCALLINIINEKVFAFLWCWYMILAIITTCSFIYWIANSFIHSEKVDYVMKFIQIAESSEYKKLQKFEKDATVERLYTVIAFAPHLLDSFVSDFLKSDGILMLRMISNHAGDMIVVQLVRNLWQEYRER 401
[Close]
BLAST of Innexin vs. UniProt/SwissProt
Match: sp|O61715|INX19_CAEEL (Innexin-19 OS=Caenorhabditis elegans OX=6239 GN=inx-19 PE=1 SV=2)

HSP 1 Score: 130.183 bits (326), Expect = 2.014e-31
Identity = 96/311 (30.87%), Postives = 160/311 (51.45%), Query Frame = 2 
Query:  437 YYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRL-GAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNGETKQES-IFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQK------------------NLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEK 1309
            YYQW+PFIL  +A+   +P + W+L    + G NI  L++ A           L +     K +EA+     D+    S  GR   R         + L G  L + + F K+LY +N ++Q  ++   L    S + L FG ++L++ + G    E+  FP+   C  ++R++  +N    QCAL  N+INEK+F FLW W +I++IIT+ S + WI  S++   K  ++ K+++I + +++K +QK                  +LL  F   FLKS G+ +LRMI+ ++GD+I   +V  LW  F+E+
Sbjct:  117 YYQWVPFILIAEALMFSLPCIFWRLCSFQS-GLNIQTLINAACDG------QALLDASDRQKAVEAITTNFVDNLDLQSPNGRIRARGWIARIKFSRFLSGQCLSILHSFTKLLYSMNVVAQFLILNACL---KSSDFLFFGFQVLNDIWAGRPWTETGHFPRVTLCDFEVRYLANLNRYTVQCALLINIINEKVFAFLWCWYMILAIITTCSFIYWIANSFIHSEKVDYVMKFIQIAESSEFKKLQKFEKDATVERLYTVIAFAPHLLDTFVSDFLKSDGVLMLRMISNHAGDMIVVQLVRNLWQEFRER 417
[Close]
BLAST of Innexin vs. UniProt/SwissProt
Match: sp|Q19746|INX3_CAEEL (Innexin-3 OS=Caenorhabditis elegans OX=6239 GN=inx-3 PE=1 SV=2)

HSP 1 Score: 117.472 bits (293), Expect = 3.386e-27
Identity = 80/285 (28.07%), Postives = 144/285 (50.53%), Query Frame = 2 
Query:  437 YYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNGETKQES-IFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQL 1288
            YYQW+P +L +QA   ++P  +W  LY    G +  +++S+A+    +D E+R K V  L   +  +L     D  + + +GR  C +       GK LG+   + Y  IK++YL N   Q  ++   L     +   L+G    ++ + G   Q+S +FP+   C   +R +  V+    QC L  N+ NEK+++F+WFW + V I T I+ L  I+      ++  +++  L    +N +KD +K ++  F    LK  G+ ++R I  ++G ++T +I  +L
Sbjct:  101 YYQWVPIVLAIQAFMFYLPSWIWSSLY-KQCGLDFPSVISEAEALRSQDSETRTKGVNKLVDFIGDIL-----DTRSKNEYGRFYCYR------FGKGLGSMTSMLYICIKLMYLANVFVQFIILNKFL----GNETFLWGFHTFADLYAGREWQDSGVFPRVTLCDFSVRKLANVHRYTVQCVLMINMFNEKIYLFIWFWFVFVLITTFINTLCTIYRLSFDSSRHNYIRSLLS-GPVNNFKD-EKAMIASFANNGLKQDGVLLMRFIDDHAGAMVTKEICEEL 367
[Close]
BLAST of Innexin vs. TrEMBL
Match: A0A4S2LNY2 (Innexin OS=Opisthorchis felineus OX=147828 GN=inx PE=3 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 1.514e-67
Identity = 130/363 (35.81%), Postives = 216/363 (59.50%), Query Frame = 2 
Query:  431 FIYYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNGETKQES-IFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEKYAIKLFDRGXXXXXXXXXXXXXXXXXXIKSEKESFLLKLN------TNCERNEQKSALPNAPPLKNDFK 1498
             +YYQW+PF+L LQ I  ++P ++W+++  N  G +I +LV  A +AV   ++ R K ++H+A+ LE MLF+ R+  +   S  R    K     ++ KRLG  L   Y FIK LYL+NA+ Q+F++Q  L     DN+  FG+ +  +  +G+  Q + +FP+ G+C V +RH+G  N+V  QC LP N++NE++++FLWFW+++ + +T+ISI  W      Q ++T F+KKYLK+ ++   KD  + +++KF+++FL+  G+F+LRMIA N+GDLI +DIV QLW IFK +Y  +     +                  + ++E   ++L+      T+ ER    S  P APP+ ++ +
Sbjct:  104 LLYYQWVPFVLGLQCIMFYVPRVIWQMICYNRTGTDIQHLVLSANQAVHATDDQRTKMIQHVARTLEQMLFQHREYRNDVWSHIRRRLWKTCSLLVVSKRLGTRLFAIYLFIKCLYLVNAVGQVFMMQTFLGLR-YDNYSFFGIAIARDILSGKDWQRTLVFPRVGYCLVPVRHMGASNYVTGQCVLPVNMLNERIYVFLWFWIVLAATLTAISIPTWFMRMSYQKSRTCFIKKYLKLGEVLTKKD--RGMVEKFKRQFLRQDGIFLLRMIAINAGDLICSDIVCQLWKIFKTRYFFRDLTMSD------------------EEQEEELHVQLSRGTVEVTDLERCASDST-PTAPPIDDEVE 444
[Close]
BLAST of Innexin vs. TrEMBL
Match: G7YJJ0 (Innexin OS=Clonorchis sinensis OX=79923 GN=inx PE=3 SV=1)

HSP 1 Score: 233.417 bits (594), Expect = 4.397e-67
Identity = 130/363 (35.81%), Postives = 215/363 (59.23%), Query Frame = 2 
Query:  431 FIYYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNGETKQES-IFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEKYAIKLFDRGXXXXXXXXXXXXXXXXXXIKSEKESFLLKLN------TNCERNEQKSALPNAPPLKNDFK 1498
             +YYQW+PF+L LQ I  ++P ++W+++  N  G +I +LV  A +AV   ++ R K ++H+A+ LE MLF+ R+  +   S  R    K     ++ KRLG  L   Y FIK LYL+NA+ Q+F++Q  L     DN+  FG+ +  +  +G+  Q + +FP+ G+C V +RH+G  N+V  QC LP N++NE++++FLWFW+++ + +T+ISI  W      Q ++T F+KKYLK+ ++   KD  + +++KF+++FL+  G+F+LRMIA N+GDLI +DIV QLW IFK +Y  +     +                  + ++E   ++L+      T+ ER       P APP+ +D +
Sbjct:  104 LLYYQWVPFVLGLQCIMFYVPRVIWQMICYNRTGTDIQHLVLSANQAVHATDDQRTKMIQHVARTLEQMLFQHREYRNDVWSHIRRRLWKTCSLLVVSKRLGTRLFAIYLFIKCLYLVNAVGQVFMMQTFLGLR-YDNYSFFGIAIARDILSGKDWQRTLVFPRVGYCLVPVRHMGASNYVTGQCVLPVNMLNERIYVFLWFWIVLAATLTAISIPTWFMRMSYQKSRTCFIKKYLKLGEVLTKKD--RGMVEKFKRQFLRQDGIFLLRMIAINAGDLICSDIVCQLWKIFKTRYFFRDLTMSD------------------EEQEEEHRVQLSRGTVEVTDLERCVSDPT-PTAPPVDDDVE 444
[Close]
BLAST of Innexin vs. TrEMBL
Match: A0A3Q0KLG6 (Innexin OS=Schistosoma mansoni OX=6183 GN=inx PE=3 SV=1)

HSP 1 Score: 216.083 bits (549), Expect = 3.422e-60
Identity = 127/295 (43.05%), Postives = 190/295 (64.41%), Query Frame = 2 
Query:  431 FIYYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNG-ETKQESIFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEKY 1312
             +YYQW+PF+L LQ I  ++P L+W+++  N  G ++ +LV  A +AV  ++E R K V+HLAK LE +LF+ R+ +       R   +K      + KRLG  L   Y FIK LYLLNAI Q+F++Q  L   S  N+ LFGV +  N   G + +   IFP+ GFC V L+H G  N+  +QC LP N++NE++++FLWFW+++ + IT+ISI  W      + ++T F+KKYLK+ +    KD  K +++KF   FL++ G+F+LRMIA N+G+LI ++IV QLW+IF+EKY
Sbjct:  104 LLYYQWVPFVLGLQCILFYLPRLIWQMICYNRVGTDVQHLVLCANQAVHANDEQRTKMVQHLAKTLEQLLFQQREYHHGLWPRVRRRMKKWGYLFFVSKRLGTRLFGIYLFIKCLYLLNAIGQIFMMQSFLGLKS--NYTLFGVAISRNILAGLDWEVTMIFPRVGFCLVPLKHFGSNNYATAQCVLPVNMLNERIYMFLWFWIVLAATITAISIPTWFTRMSYEKSRTHFIKKYLKLGEKVSRKD--KYMVEKFTLLFLRNDGVFLLRMIAINAGELICSEIVCQLWHIFREKY 394
[Close]
BLAST of Innexin vs. TrEMBL
Match: A0A0X3PXF2 (Innexin OS=Schistocephalus solidus OX=70667 GN=inx PE=3 SV=1)

HSP 1 Score: 215.312 bits (547), Expect = 5.822e-60
Identity = 111/297 (37.37%), Postives = 195/297 (65.66%), Query Frame = 2 
Query:  431 FIYYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNGETKQES-IFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKI--KDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEKY 1312
             +YYQW+PF+L LQ I  +IP ++W+++  N  G ++ +L++ A +A     + R + V+HLAK LE +LF+ R+  +  ++  R +C K+     + KRLG +L   Y FIK+LYL N++ QL+L+Q  L  +SS  +   G  + +N   G   Q + +FP+ GFC V +RH+ G  +  +QCALP N++NE++++FLWFW+++ + +T+ISI +W      + ++T F++++L++   + N+Y    + ++ KF ++FL+  G+F+LRM+A N+G+LI A+IV++LW I+K+KY
Sbjct:  104 LLYYQWVPFVLGLQCILFYIPRIIWQMICYNRTGTDLQHLINLASEAAHATSDKRAETVKHLAKSLEQLLFQHREYRTGWATKVRKVCYKVCRLFYISKRLGTHLFGTYLFIKLLYLANSVGQLYLMQNFLGLNSS-QYTYLGAAIATNILTGRDWQVTLVFPRVGFCVVPIRHLVGPVYATAQCALPMNMLNERIYMFLWFWVLLSAFLTAISIPMWFMRMAYEKSRTNFVRRFLRVGYDEENRYSSKDRCMVAKFTRQFLRHDGIFLLRMVAINAGELIAAEIVHKLWEIYKQKY 399
[Close]
BLAST of Innexin vs. TrEMBL
Match: G4V9H2 (Innexin OS=Schistosoma mansoni OX=6183 GN=inx PE=3 SV=1)

HSP 1 Score: 215.312 bits (547), Expect = 7.108e-60
Identity = 127/295 (43.05%), Postives = 190/295 (64.41%), Query Frame = 2 
Query:  431 FIYYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNG-ETKQESIFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEKY 1312
             +YYQW+PF+L LQ I  ++P L+W+++  N  G ++ +LV  A +AV  ++E R K V+HLAK LE +LF+ R+ +       R   +K      + KRLG  L   Y FIK LYLLNAI Q+F++Q  L   S  N+ LFGV +  N   G + +   IFP+ GFC V L+H G  N+  +QC LP N++NE++++FLWFW+++ + IT+ISI  W      + ++T F+KKYLK+ +    KD  K +++KF   FL++ G+F+LRMIA N+G+LI ++IV QLW+IF+EKY
Sbjct:  104 LLYYQWVPFVLGLQCILFYLPRLIWQMICYNRVGTDVQHLVLCANQAVHANDEQRTKMVQHLAKTLEQLLFQQREYHHGLWPRVRRRMKKWGYLFFVSKRLGTRLFGIYLFIKCLYLLNAIGQIFMMQSFLGLKS--NYTLFGVAISRNILVGLDWEVTMIFPRVGFCLVPLKHFGSNNYATAQCVLPVNMLNERIYMFLWFWIVLAATITAISIPTWFTRMSYEKSRTHFIKKYLKLGEKVSRKD--KYMVEKFTLLFLRNDGVFLLRMIAINAGELICSEIVCQLWHIFREKY 394
[Close]
BLAST of Innexin vs. Planmine SMEST
Match: SMESG000030964.1 (SMESG000030964.1)

HSP 1 Score: 624.394 bits (1609), Expect = 0.000e+0
Identity = 356/357 (99.72%), Postives = 357/357 (100.00%), Query Frame = 2 
Query:  434 IYYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNGETKQESIFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEKYAIKLFDRGXXXXXXXXXXXXXXXXXXIKSEKESFLLKLNTNCERNEQKSALPNAPPLKNDFKMV 1504
            +YYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAISQLFLIQLLLSPHSSDNHLLFGVKLLSNAFNGETKQESIFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMIIVSIITSISILVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEKYAIKLFDRGNKKKSKKKSAAHHSDNSSIKSEKESFLLKLNTNCERNEQKSALPNAPPLKNDFKMV
Sbjct:  104 LYYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAISQLFLIQLLLSPHSSDNHLLFGVKLLSNAFNGETKQESIFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMIIVSIITSISILVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEKYAIKLFDRGNKKKSKKKSAAHHSDNSSIKSEKESFLLKLNTNCERNEQKSALPNAPPLKNDFKMV 460
[Close]
BLAST of Innexin vs. Planmine SMEST
Match: SMESG000030964.1 (SMESG000030964.1)

HSP 1 Score: 623.624 bits (1607), Expect = 0.000e+0
Identity = 356/357 (99.72%), Postives = 357/357 (100.00%), Query Frame = 2 
Query:  434 IYYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNGETKQESIFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEKYAIKLFDRGXXXXXXXXXXXXXXXXXXIKSEKESFLLKLNTNCERNEQKSALPNAPPLKNDFKMV 1504
            +YYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAISQLFLIQLLLSPHSSDNHLLFGVKLLSNAFNGETKQESIFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMIIVSIITSISILVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEKYAIKLFDRGNKKKSKKKSAAHHSDNSSIKSEKESFLLKLNTNCERNEQKSALPNAPPLKNDFKMV
Sbjct:   68 LYYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAISQLFLIQLLLSPHSSDNHLLFGVKLLSNAFNGETKQESIFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMIIVSIITSISILVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEKYAIKLFDRGNKKKSKKKSAAHHSDNSSIKSEKESFLLKLNTNCERNEQKSALPNAPPLKNDFKMV 424
[Close]
BLAST of Innexin vs. Planmine SMEST
Match: SMESG000030971.1 (SMESG000030971.1)

HSP 1 Score: 598.201 bits (1541), Expect = 0.000e+0
Identity = 343/357 (96.08%), Postives = 349/357 (97.76%), Query Frame = 2 
Query:  434 IYYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNGETKQESIFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEKYAIKLFDRGXXXXXXXXXXXXXXXXXXIKSEKESFLLKLNTNCERNEQKSALPNAPPLKNDFKMV 1504
            +Y QWIPFIL LQA++CFIPHLLWKLLYINNNG+NIDNL+SKA KAVV DEESRLKEVRHLAKVLEAMLFEIRDD SANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAISQLFLIQLLLSPHSSDNHLLFGVKLLSNAFNGETKQESIFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMIIVSIITSISILVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEKYAIKLFDRGNKKKSKKKSAAHHSDNSSIKSEKESFLLKLNTNCERNEQKS  PNAPPLK+D KMV
Sbjct:  104 LYDQWIPFILTLQAMACFIPHLLWKLLYINNNGKNIDNLMSKASKAVVMDEESRLKEVRHLAKVLEAMLFEIRDDKSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAISQLFLIQLLLSPHSSDNHLLFGVKLLSNAFNGETKQESIFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMIIVSIITSISILVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEKYAIKLFDRGNKKKSKKKSAAHHSDNSSIKSEKESFLLKLNTNCERNEQKSPSPNAPPLKDDSKMV 460
[Close]
BLAST of Innexin vs. Planmine SMEST
Match: SMESG000070656.1 (SMESG000070656.1)

HSP 1 Score: 229.565 bits (584), Expect = 1.447e-69
Identity = 122/299 (40.80%), Postives = 194/299 (64.88%), Query Frame = 2 
Query:  434 IYYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNGET-KQESIFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEKYAIKLF 1327
            +YYQW+PF+L LQAI  +IP L+W++   N  G +I +L+  A +AV   +E+R K V ++AK +E ML++ RD+ +   +  R    KI  FCI+ KR G  L+  YF IK LYL N I QL+ ++  L  + +   + FG K++ N  NG+  K   +FP+ G+C+V +++VG  N +V+QC LP N++NEK+F FLW W+   +I+ +ISI++WIF + +   +  F+ ++LK+    +  D +K ++ KFE +FL+  G+F+L+MI+ N+GD++ ADIV QLW IFK +YA + F
Sbjct:   59 LYYQWVPFLLSLQAIMFYIPRLIWQIFCYNTTGTDIHHLIVSANQAVHSSDEARTKMVNYIAKTIEQMLYQHRDNRNGRLARFRRSLYKICCFCIMSKRQGTRLLCMYFIIKFLYLTNCILQLYFMKCFLGLNEAG--IFFGFKIMKNIINGQDWKSTLVFPRVGYCYVNVKNVGTTNALVAQCVLPVNMLNEKIFFFLWCWIFSSAILLTISIIIWIFRTSVYQRRRGFIYRFLKLNQQLKNCDEEKCMVVKFEHQFLRYDGVFLLKMISLNAGDIVAADIVCQLWKIFKSQYANRNF 355
[Close]
BLAST of Innexin vs. Planmine SMEST
Match: SMESG000030970.1 (SMESG000030970.1)

HSP 1 Score: 224.557 bits (571), Expect = 1.444e-67
Identity = 123/295 (41.69%), Postives = 198/295 (67.12%), Query Frame = 2 
Query:  434 IYYQWIPFILQLQAISCFIPHLLWKLLYINNNGQNIDNLVSKAKKAVVKDEESRLKEVRHLAKVLEAMLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLLNAXXXXXXXXXXXXPHSSDNHLLFGVKLLSNAFNGETKQES-IFPKFGFCFVQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMXXXXXXXXXXXLVWIFESYLQYNKTAFLKKYLKIKDLNQYKDVQ-KNLLKKFEKRFLKSSGLFILRMIATNSGDLITADIVNQLWNIFKEKY 1312
            +YYQW+PF L  QA+ C+IPH++W+      N  + ++++ +A +A+  D+E R  EV HLA+ L+ +LF+ R  +S  +S  R    +I+   I   ++G  L+++Y  IKILY+ NA+ Q++L++  L  +SSD +  FG K+L++  +G+  Q S IFP+ GFC  + RH+G  N+V +QCALP N++NEK++IFLWFWM+I+S+I+  S+ +W+    +  NKT FL  YLK+     +++++ K  + KFEK FL++ G+F LR++ATN GDLITA++V+QLW I+   Y
Sbjct:  104 LYYQWVPFALYGQAVMCYIPHIIWRFFRKRQNNADFNHIIQRANRAIELDDEKRKNEVIHLAQALKNILFQNRTCDS--TSVLRREANRIHSKIIEWIKMGIPLVIYYLVIKILYVTNAVGQIYLMKSFLRLNSSD-YKFFGSKILNDIIHGKNWQVSKIFPRVGFCLGKFRHLGATNYVTAQCALPLNMLNEKVYIFLWFWMLILSVISITSLFMWLIRMLISRNKTDFLNMYLKLSGDYTHRELKDKKKIVKFEKIFLRNDGMFFLRILATNVGDLITAEVVSQLWKIYAPCY 395
The following BLAST results are available for this feature:
BLAST of Innexin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
inx-15.379e-4032.89Innexin [Source:UniProtKB/TrEMBL;Acc:Q17394][more]
unc-79.190e-4032.76Innexin unc-7 [Source:UniProtKB/Swiss-Prot;Acc:Q0... [more]
inx-19.771e-4032.88Innexin [Source:UniProtKB/TrEMBL;Acc:Q17394][more]
inx-19.771e-4032.88Innexin [Source:UniProtKB/TrEMBL;Acc:Q17394][more]
unc-71.059e-3932.76Innexin unc-7 [Source:UniProtKB/Swiss-Prot;Acc:Q0... [more]
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BLAST of Innexin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
shakB4.329e-626.44gene:FBgn0085387 transcript:FBtr0114645[more]
shakB7.997e-626.44gene:FBgn0085387 transcript:FBtr0309369[more]
shakB7.997e-626.44gene:FBgn0085387 transcript:FBtr0309370[more]
shakB7.997e-626.44gene:FBgn0085387 transcript:FBtr0342859[more]
shakB8.022e-626.44gene:FBgn0085387 transcript:FBtr0112570[more]
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BLAST of Innexin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q03412|UNC7_CAEEL2.565e-3731.38Innexin unc-7 OS=Caenorhabditis elegans OX=6239 GN... [more]
sp|O01393|UNC9_CAEEL5.955e-3431.77Innexin unc-9 OS=Caenorhabditis elegans OX=6239 GN... [more]
sp|A8WVX4|INX19_CAEBR6.246e-3231.51Innexin-19 OS=Caenorhabditis briggsae OX=6238 GN=i... [more]
sp|O61715|INX19_CAEEL2.014e-3130.87Innexin-19 OS=Caenorhabditis elegans OX=6239 GN=in... [more]
sp|Q19746|INX3_CAEEL3.386e-2728.07Innexin-3 OS=Caenorhabditis elegans OX=6239 GN=inx... [more]
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BLAST of Innexin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4S2LNY21.514e-6735.81Innexin OS=Opisthorchis felineus OX=147828 GN=inx ... [more]
G7YJJ04.397e-6735.81Innexin OS=Clonorchis sinensis OX=79923 GN=inx PE=... [more]
A0A3Q0KLG63.422e-6043.05Innexin OS=Schistosoma mansoni OX=6183 GN=inx PE=3... [more]
A0A0X3PXF25.822e-6037.37Innexin OS=Schistocephalus solidus OX=70667 GN=inx... [more]
G4V9H27.108e-6043.05Innexin OS=Schistosoma mansoni OX=6183 GN=inx PE=3... [more]
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BLAST of Innexin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000030964.10.000e+099.72SMESG000030964.1[more]
SMESG000030964.10.000e+099.72SMESG000030964.1[more]
SMESG000030971.10.000e+096.08SMESG000030971.1[more]
SMESG000070656.11.447e-6940.80SMESG000070656.1[more]
SMESG000030970.11.444e-6741.69SMESG000030970.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30027019 ID=SMED30027019|Name=Innexin|organism=Schmidtea mediterranea sexual|type=transcript|length=1593bp
CCCATACGACAAAGATTCAAGATTCAAAGTCAAACAATCAGATAATATTG
TATCAAATCCATCAAATATCCAATTAAATTCACCACAAGGTAATGGTTGA
CTTAAAGCCCAATCGTAAAGATTGATAATATCAATATAGAGCAACTATGT
GTTTTCATCAGTTTTAATAAAGTGTTTACTAACAAAATTATTAATTTAAT
TGGTTCATAAAAAAAGTCAGCCAAAAGAAAACAGTCAATTTTCAAATACA
CCTTCATATAGTCAAGCAAATTTATACACCTAGATTCATCCCAGTTTACT
AGAGCTTTCTGATACTTATCGTCACTGACTTTCCCAAATCGGTGGTGACT
CATAAGTTTCTTAAAGAAATTCTAAGGAGATCTCAAAGTTATAACTACCT
AACTACCTACTGCAAAGGCTTATTTTATTATTTATATATTACCAATGGAT
TCCTTTTATATTACAATTGCAAGCCATTTCGTGTTTTATTCCGCATCTGT
TATGGAAGCTATTATACATCAACAACAATGGGCAAAATATTGATAATCTG
GTGAGTAAAGCTAAAAAGGCAGTCGTGAAAGATGAAGAATCGAGATTAAA
AGAAGTTCGTCATCTAGCAAAGGTTCTCGAAGCAATGCTATTTGAGATCC
GTGACGACAATAGCGCAAATTCCTCATTCGGCAGAAATTTGTGCAGAAAG
ATTTATGAATTTTGCATTCTTGGAAAGCGGCTTGGGGCTTATTTAATTGT
CTTTTACTTCTTCATCAAAATTCTCTATTTGCTGAACGCCATTTCTCAAT
TATTTTTAATTCAATTATTACTGTCGCCACATTCTAGTGACAATCATTTA
CTTTTTGGAGTAAAGCTTCTCTCAAATGCTTTTAATGGGGAGACCAAGCA
GGAGTCCATATTTCCGAAATTCGGATTTTGTTTTGTACAATTAAGGCATG
TCGGTGGGGTTAATTTTGTCGTTTCTCAATGTGCTTTGCCCGCAAACGTG
ATAAATGAGAAAATGTTTATATTTCTCTGGTTCTGGATGATAATCGTTTC
AATCATAACATCTATTAGCATTTTGGTTTGGATCTTTGAATCTTACCTCC
AATATAATAAAACGGCTTTCTTGAAGAAATATCTAAAAATCAAAGATCTG
AATCAATACAAAGATGTGCAAAAGAATCTTTTGAAAAAATTCGAAAAGAG
ATTTCTCAAAAGCAGTGGATTATTTATTCTGAGAATGATAGCCACCAATT
CTGGAGACTTGATAACTGCAGATATTGTCAATCAGCTGTGGAACATTTTC
AAAGAAAAGTACGCAATCAAGTTATTTGATAGAGGAAACAAAAAGAAAAG
TAAAAAGAAATCTGCAGCGCATCACAGTGACAACAGCTCAATCAAATCGG
AAAAAGAATCCTTTTTATTAAAGCTGAACACCAATTGTGAACGGAATGAA
CAGAAGTCTGCTTTACCAAATGCCCCTCCGTTGAAAAATGATTTTAAAAT
GGTTTAGAGTGCTTTGTTTTAATGAATCGCTCAGAATGATATTTCAGAAA
TTTATTGTTATTAAAACAAAATAATACAAATAATACTTTACTC
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protein sequence of SMED30027019-orf-1

>SMED30027019-orf-1 ID=SMED30027019-orf-1|Name=SMED30027019-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=291bp
MLFEIRDDNSANSSFGRNLCRKIYEFCILGKRLGAYLIVFYFFIKILYLL
NAISQLFLIQLLLSPHSSDNHLLFGVKLLSNAFNGETKQESIFPKFGFCF
VQLRHVGGVNFVVSQCALPANVINEKMFIFLWFWMIIVSIITSISILVWI
FESYLQYNKTAFLKKYLKIKDLNQYKDVQKNLLKKFEKRFLKSSGLFILR
MIATNSGDLITADIVNQLWNIFKEKYAIKLFDRGNKKKSKKKSAAHHSDN
SSIKSEKESFLLKLNTNCERNEQKSALPNAPPLKNDFKMV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000419prepharyngeal region
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR000990Innexin
Vocabulary: cellular component
TermDefinition
GO:0005921gap junction
GO:0005886plasma membrane
GO:0016020membrane
GO:0016021integral component of membrane
GO:0030054cell junction
Vocabulary: biological process
TermDefinition
GO:0006810transport
GO:0006811ion transport