Non-specific serine/threonine protein kinase
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30027002 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Human
Match: PAK1 (p21 (RAC1) activated kinase 1 [Source:HGNC Symbol;Acc:HGNC:8590]) HSP 1 Score: 352.058 bits (902), Expect = 4.902e-112 Identity = 169/278 (60.79%), Postives = 205/278 (73.74%), Query Frame = 2
Query: 839 SPLLEVETDELGETGDSVTPCDKSKDSVSIEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+P +V T + T ++ TP D R+ + ++ S E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 114 TPTRDVATSPISPTENNTTPPDA---------------LTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALY 376 HSP 2 Score: 65.4698 bits (158), Expect = 2.388e-10 Identity = 29/48 (60.42%), Postives = 38/48 (79.17%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLK 1799
L ALYLIATNG P L++P +S +F DFL+RCLE+D++KR SA LL+
Sbjct: 372 LRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQ 419
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Human
Match: PAK1 (p21 (RAC1) activated kinase 1 [Source:HGNC Symbol;Acc:HGNC:8590]) HSP 1 Score: 352.443 bits (903), Expect = 2.595e-111 Identity = 169/278 (60.79%), Postives = 205/278 (73.74%), Query Frame = 2
Query: 839 SPLLEVETDELGETGDSVTPCDKSKDSVSIEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+P +V T + T ++ TP D R+ + ++ S E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 212 TPTRDVATSPISPTENNTTPPDA---------------LTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALY 474 HSP 2 Score: 68.9366 bits (167), Expect = 1.961e-11 Identity = 30/55 (54.55%), Postives = 42/55 (76.36%), Query Frame = 3
Query: 1638 HISMKILCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKH 1802
+++ L ALYLIATNG P L++P +S +F DFL+RCLE+D++KR SA LL+H
Sbjct: 464 YLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQH 518 HSP 3 Score: 59.3066 bits (142), Expect = 2.584e-8 Identity = 25/50 (50.00%), Postives = 33/50 (66.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K+EQK NP+
Sbjct: 73 PEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQ 122
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Human
Match: PAK1 (p21 (RAC1) activated kinase 1 [Source:HGNC Symbol;Acc:HGNC:8590]) HSP 1 Score: 352.829 bits (904), Expect = 4.033e-111 Identity = 169/278 (60.79%), Postives = 205/278 (73.74%), Query Frame = 2
Query: 839 SPLLEVETDELGETGDSVTPCDKSKDSVSIEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+P +V T + T ++ TP D R+ + ++ S E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 212 TPTRDVATSPISPTENNTTPPDA---------------LTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALY 474 HSP 2 Score: 82.8037 bits (203), Expect = 9.133e-16 Identity = 40/80 (50.00%), Postives = 59/80 (73.75%), Query Frame = 3
Query: 1638 HISMKILCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSE 1877
+++ L ALYLIATNG P L++P +S +F DFL+RCLE+D++KR SA LL+H+F+ +KP+S L PLI A+E ++
Sbjct: 464 YLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFLK-IAKPLSSLTPLIAAAKEATK 542 HSP 3 Score: 58.9214 bits (141), Expect = 2.766e-8 Identity = 25/50 (50.00%), Postives = 33/50 (66.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K+EQK NP+
Sbjct: 73 PEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQ 122
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Human
Match: PAK1 (p21 (RAC1) activated kinase 1 [Source:HGNC Symbol;Acc:HGNC:8590]) HSP 1 Score: 351.673 bits (901), Expect = 1.279e-110 Identity = 169/278 (60.79%), Postives = 205/278 (73.74%), Query Frame = 2
Query: 839 SPLLEVETDELGETGDSVTPCDKSKDSVSIEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+P +V T + T ++ TP D R+ + ++ S E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 212 TPTRDVATSPISPTENNTTPPDA---------------LTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALY 474 HSP 2 Score: 65.4698 bits (158), Expect = 2.967e-10 Identity = 29/54 (53.70%), Postives = 41/54 (75.93%), Query Frame = 3
Query: 1638 HISMKILCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLK 1799
+++ L ALYLIATNG P L++P +S +F DFL+RCLE+D++KR SA LL+
Sbjct: 464 YLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQ 517 HSP 3 Score: 58.9214 bits (141), Expect = 2.725e-8 Identity = 25/50 (50.00%), Postives = 33/50 (66.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K+EQK NP+
Sbjct: 73 PEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQ 122
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Human
Match: PAK3 (p21 (RAC1) activated kinase 3 [Source:HGNC Symbol;Acc:HGNC:8592]) HSP 1 Score: 350.903 bits (899), Expect = 1.910e-110 Identity = 163/246 (66.26%), Postives = 195/246 (79.27%), Query Frame = 2
Query: 935 NQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
N + + R+ +R + E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 227 NANSSTLYRNTDRQRKKSKMTDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTALDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 472 HSP 2 Score: 82.8037 bits (203), Expect = 1.075e-15 Identity = 40/77 (51.95%), Postives = 57/77 (74.03%), Query Frame = 3
Query: 1638 HISMKILCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
+++ L ALYLIATNG P L++P +S +F DFL+RCLE+D+D+R SA LL+H F+ +KP+S L PLI+ A+E
Sbjct: 462 YLNENPLRALYLIATNGTPELQNPERLSAVFRDFLNRCLEMDVDRRGSAKELLQHPFLK-LAKPLSSLTPLIIAAKE 537 HSP 3 Score: 58.151 bits (139), Expect = 5.319e-8 Identity = 25/50 (50.00%), Postives = 32/50 (64.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K EQK NP+
Sbjct: 68 PEISLPSDFEHTIHVGFDAVTGEFTGIPEQWARLLQTSNITKLEQKKNPQ 117
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Celegans
Match: pak-1 (Serine/threonine-protein kinase pak-1 [Source:UniProtKB/Swiss-Prot;Acc:Q17850]) HSP 1 Score: 334.724 bits (857), Expect = 6.456e-105 Identity = 156/233 (66.95%), Postives = 190/233 (81.55%), Query Frame = 2
Query: 974 RRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
++A + + ++KL+ +VS +P KY +D+IG GASG V ++ T+ +VAIKQMNLK QPKK+LI+NEILV++ NKH NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE M++ IA +CREV AL FLHS HVIHRDIKSDNILLG+DGSVK+ DFGFCAQL + R+TMVGTPYWMAPEVVTRKQYGPKVD+WSLGIM IEM+EGEPPYLNENPLRA++
Sbjct: 218 QKAKKKMTDAEVLTKLRTIVSIGNPDRKYRKVDKIGSGASGSVYTAIEISTEAEVAIKQMNLKDQPKKELIINEILVMRENKHANIVNYLDSYLVCDELWVVMEYLAGGSLTDVVTECQMEDGIIAAVCREVLQALEFLHSRHVIHRDIKSDNILLGMDGSVKLTDFGFCAQLSPEQRKRTTMVGTPYWMAPEVVTRKQYGPKVDVWSLGIMAIEMVEGEPPYLNENPLRAIY 450 HSP 2 Score: 57.7658 bits (138), Expect = 2.296e-8 Identity = 33/71 (46.48%), Postives = 47/71 (66.20%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
L A+YLIATNGKP +++ LF DF+D LEV ++ R SAS LL H F+ +KP++ L LI+ A++
Sbjct: 446 LRAIYLIATNGKPDFPGRDSMTLLFKDFVDSALEVQVENRWSASQLLTHPFLR-CAKPLASLYYLIVAAKK 515 HSP 3 Score: 56.225 bits (134), Expect = 7.817e-8 Identity = 24/50 (48.00%), Postives = 31/50 (62.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P IS P+ H HV +DP TG+F G+P +W L S ISK EQ+ NP+
Sbjct: 19 PVISRPSNFEHTIHVGYDPKTGEFTGMPEAWARLLTDSQISKQEQQQNPQ 68
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Celegans
Match: pak-1 (Serine/threonine-protein kinase pak-1 [Source:UniProtKB/Swiss-Prot;Acc:Q17850]) HSP 1 Score: 335.88 bits (860), Expect = 7.231e-105 Identity = 156/233 (66.95%), Postives = 190/233 (81.55%), Query Frame = 2
Query: 974 RRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
++A + + ++KL+ +VS +P KY +D+IG GASG V ++ T+ +VAIKQMNLK QPKK+LI+NEILV++ NKH NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE M++ IA +CREV AL FLHS HVIHRDIKSDNILLG+DGSVK+ DFGFCAQL + R+TMVGTPYWMAPEVVTRKQYGPKVD+WSLGIM IEM+EGEPPYLNENPLRA++
Sbjct: 264 QKAKKKMTDAEVLTKLRTIVSIGNPDRKYRKVDKIGSGASGSVYTAIEISTEAEVAIKQMNLKDQPKKELIINEILVMRENKHANIVNYLDSYLVCDELWVVMEYLAGGSLTDVVTECQMEDGIIAAVCREVLQALEFLHSRHVIHRDIKSDNILLGMDGSVKLTDFGFCAQLSPEQRKRTTMVGTPYWMAPEVVTRKQYGPKVDVWSLGIMAIEMVEGEPPYLNENPLRAIY 496 HSP 2 Score: 59.3066 bits (142), Expect = 9.388e-9 Identity = 43/107 (40.19%), Postives = 56/107 (52.34%), Query Frame = 2
Query: 77 ELPPAPPIRSSSTLRRKDRYSVSXXXXXXXXXXXXXXXXXXXXXXXFNYND------PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
E PPAPPIR SS+ R+++ V + KKKKT K N + P IS P+ H HV +DP TG+F G+P +W L S ISK EQ+ NP+
Sbjct: 9 EKPPAPPIRFSSSATRENQV-VGLKPLPKEPEATKKKKTMPNPFMKKNKDKKEASEKPVISRPSNFEHTIHVGYDPKTGEFTGMPEAWARLLTDSQISKQEQQQNPQ 114 HSP 3 Score: 57.3806 bits (137), Expect = 3.241e-8 Identity = 33/71 (46.48%), Postives = 47/71 (66.20%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
L A+YLIATNGKP +++ LF DF+D LEV ++ R SAS LL H F+ +KP++ L LI+ A++
Sbjct: 492 LRAIYLIATNGKPDFPGRDSMTLLFKDFVDSALEVQVENRWSASQLLTHPFLR-CAKPLASLYYLIVAAKK 561
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Celegans
Match: pak-1 (Serine/threonine-protein kinase pak-1 [Source:UniProtKB/Swiss-Prot;Acc:Q17850]) HSP 1 Score: 335.495 bits (859), Expect = 1.186e-104 Identity = 156/233 (66.95%), Postives = 190/233 (81.55%), Query Frame = 2
Query: 974 RRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
++A + + ++KL+ +VS +P KY +D+IG GASG V ++ T+ +VAIKQMNLK QPKK+LI+NEILV++ NKH NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE M++ IA +CREV AL FLHS HVIHRDIKSDNILLG+DGSVK+ DFGFCAQL + R+TMVGTPYWMAPEVVTRKQYGPKVD+WSLGIM IEM+EGEPPYLNENPLRA++
Sbjct: 267 QKAKKKMTDAEVLTKLRTIVSIGNPDRKYRKVDKIGSGASGSVYTAIEISTEAEVAIKQMNLKDQPKKELIINEILVMRENKHANIVNYLDSYLVCDELWVVMEYLAGGSLTDVVTECQMEDGIIAAVCREVLQALEFLHSRHVIHRDIKSDNILLGMDGSVKLTDFGFCAQLSPEQRKRTTMVGTPYWMAPEVVTRKQYGPKVDVWSLGIMAIEMVEGEPPYLNENPLRAIY 499 HSP 2 Score: 59.3066 bits (142), Expect = 9.837e-9 Identity = 43/107 (40.19%), Postives = 56/107 (52.34%), Query Frame = 2
Query: 77 ELPPAPPIRSSSTLRRKDRYSVSXXXXXXXXXXXXXXXXXXXXXXXFNYND------PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
E PPAPPIR SS+ R+++ V + KKKKT K N + P IS P+ H HV +DP TG+F G+P +W L S ISK EQ+ NP+
Sbjct: 9 EKPPAPPIRFSSSATRENQV-VGLKPLPKEPEATKKKKTMPNPFMKKNKDKKEASEKPVISRPSNFEHTIHVGYDPKTGEFTGMPEAWARLLTDSQISKQEQQQNPQ 114 HSP 3 Score: 57.3806 bits (137), Expect = 3.253e-8 Identity = 33/71 (46.48%), Postives = 47/71 (66.20%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
L A+YLIATNGKP +++ LF DF+D LEV ++ R SAS LL H F+ +KP++ L LI+ A++
Sbjct: 495 LRAIYLIATNGKPDFPGRDSMTLLFKDFVDSALEVQVENRWSASQLLTHPFLR-CAKPLASLYYLIVAAKK 564
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Celegans
Match: max-2 (Serine/threonine-protein kinase max-2 [Source:UniProtKB/Swiss-Prot;Acc:G5EGQ3]) HSP 1 Score: 254.603 bits (649), Expect = 7.422e-82 Identity = 115/222 (51.80%), Postives = 169/222 (76.13%), Query Frame = 2
Query: 1010 ISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMN-NELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+++L+ +V+ S+P KYE+ +IG GASG V + VA+K+M K QPKK+++L EI V+K +H N+VNY++S++++ ++LWVVM+YL+GG+LTDVV + +DE QIA + +E AL FLH + ++HRDIKSDN+LLG++G VK+ D GFCAQ+ G + R T+VGTPYWM+PE++ +KQY KVD+WSLGIM +EM++GEPPYL E PL+A++
Sbjct: 363 LNQLREIVNPSNPLGKYEMKKQIGVGASGTVFVANVAGSTDVVAVKRMAFKTQPKKEMLLTEIKVMKQYRHPNLVNYIESYLVDADDLWVVMDYLEGGNLTDVVVKTELDEGQIAAVLQECLKALHFLHRHSIVHRDIKSDNVLLGMNGEVKLTDMGFCAQIQPG-SKRDTVVGTPYWMSPEILNKKQYNYKVDIWSLGIMALEMIDGEPPYLRETPLKAIY 583 HSP 2 Score: 70.4774 bits (171), Expect = 7.422e-82 Identity = 37/72 (51.39%), Postives = 48/72 (66.67%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQ 1871
L A+YLIA NGKP +K +S F +FLD+CL VD D+RA + LL H F+ K+KP+S LIP I RE+
Sbjct: 579 LKAIYLIAQNGKPEIKQRDRLSSEFNNFLDKCLVVDPDQRADTTELLAHPFLK-KAKPLSSLIPYIRAVREK 649
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Celegans
Match: max-2 (Serine/threonine-protein kinase max-2 [Source:UniProtKB/Swiss-Prot;Acc:G5EGQ3]) HSP 1 Score: 254.603 bits (649), Expect = 7.426e-82 Identity = 115/222 (51.80%), Postives = 169/222 (76.13%), Query Frame = 2
Query: 1010 ISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMN-NELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+++L+ +V+ S+P KYE+ +IG GASG V + VA+K+M K QPKK+++L EI V+K +H N+VNY++S++++ ++LWVVM+YL+GG+LTDVV + +DE QIA + +E AL FLH + ++HRDIKSDN+LLG++G VK+ D GFCAQ+ G + R T+VGTPYWM+PE++ +KQY KVD+WSLGIM +EM++GEPPYL E PL+A++
Sbjct: 312 LNQLREIVNPSNPLGKYEMKKQIGVGASGTVFVANVAGSTDVVAVKRMAFKTQPKKEMLLTEIKVMKQYRHPNLVNYIESYLVDADDLWVVMDYLEGGNLTDVVVKTELDEGQIAAVLQECLKALHFLHRHSIVHRDIKSDNVLLGMNGEVKLTDMGFCAQIQPG-SKRDTVVGTPYWMSPEILNKKQYNYKVDIWSLGIMALEMIDGEPPYLRETPLKAIY 532 HSP 2 Score: 70.4774 bits (171), Expect = 7.426e-82 Identity = 37/72 (51.39%), Postives = 48/72 (66.67%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQ 1871
L A+YLIA NGKP +K +S F +FLD+CL VD D+RA + LL H F+ K+KP+S LIP I RE+
Sbjct: 528 LKAIYLIAQNGKPEIKQRDRLSSEFNNFLDKCLVVDPDQRADTTELLAHPFLK-KAKPLSSLIPYIRAVREK 598
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Fly
Match: Pak (gene:FBgn0267698 transcript:FBtr0334540) HSP 1 Score: 349.747 bits (896), Expect = 5.306e-113 Identity = 161/229 (70.31%), Postives = 189/229 (82.53%), Query Frame = 2
Query: 986 RHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+ S E + KL+ +VS DP KY +++IGQGASG V + T +VAIKQMNL QQPKK+LI+NEILV++ NKH N+VNYLDS++++ ELWVVMEYL GGSLTDVVTE CMDE QIA +CREV AL FLH+N VIHRDIKSDNILLGLDGSVK+ DFGFCAQ+ ++ R+TMVGTPYWMAPEVVTRKQYGPKVDLWSLGIM IEM+EGEPPYLNENPL+AL+
Sbjct: 542 KKMSDEEILEKLRTIVSVGDPNRKYTKMEKIGQGASGTVYTAIESSTGMEVAIKQMNLSQQPKKELIINEILVMRENKHPNVVNYLDSYLVSEELWVVMEYLPGGSLTDVVTETCMDEGQIAAVCREVLQALEFLHANQVIHRDIKSDNILLGLDGSVKLTDFGFCAQISPEQSKRTTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMAIEMVEGEPPYLNENPLKALY 770 HSP 2 Score: 79.337 bits (194), Expect = 5.306e-113 Identity = 40/74 (54.05%), Postives = 56/74 (75.68%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSE 1877
L ALYLIATNGKP +K+ +S F DFLD+CLEV++D+RASA LLKH F+ ++P++ L PLI+ A+E ++
Sbjct: 766 LKALYLIATNGKPEIKEKDKLSSAFQDFLDQCLEVEVDRRASALDLLKHPFL-KLARPLASLTPLIMAAKEATK 838 HSP 3 Score: 59.3066 bits (142), Expect = 1.499e-8 Identity = 27/50 (54.00%), Postives = 31/50 (62.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P IS PT H HV FD TG+F G+P +W L SNISK EQK NP+
Sbjct: 81 PNISYPTNFEHTVHVGFDAVTGEFTGMPEAWARLLMNSNISKQEQKKNPQ 130
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Fly
Match: Pak (gene:FBgn0267698 transcript:FBtr0334542) HSP 1 Score: 349.747 bits (896), Expect = 5.306e-113 Identity = 161/229 (70.31%), Postives = 189/229 (82.53%), Query Frame = 2
Query: 986 RHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+ S E + KL+ +VS DP KY +++IGQGASG V + T +VAIKQMNL QQPKK+LI+NEILV++ NKH N+VNYLDS++++ ELWVVMEYL GGSLTDVVTE CMDE QIA +CREV AL FLH+N VIHRDIKSDNILLGLDGSVK+ DFGFCAQ+ ++ R+TMVGTPYWMAPEVVTRKQYGPKVDLWSLGIM IEM+EGEPPYLNENPL+AL+
Sbjct: 542 KKMSDEEILEKLRTIVSVGDPNRKYTKMEKIGQGASGTVYTAIESSTGMEVAIKQMNLSQQPKKELIINEILVMRENKHPNVVNYLDSYLVSEELWVVMEYLPGGSLTDVVTETCMDEGQIAAVCREVLQALEFLHANQVIHRDIKSDNILLGLDGSVKLTDFGFCAQISPEQSKRTTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMAIEMVEGEPPYLNENPLKALY 770 HSP 2 Score: 79.337 bits (194), Expect = 5.306e-113 Identity = 40/74 (54.05%), Postives = 56/74 (75.68%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSE 1877
L ALYLIATNGKP +K+ +S F DFLD+CLEV++D+RASA LLKH F+ ++P++ L PLI+ A+E ++
Sbjct: 766 LKALYLIATNGKPEIKEKDKLSSAFQDFLDQCLEVEVDRRASALDLLKHPFL-KLARPLASLTPLIMAAKEATK 838 HSP 3 Score: 59.3066 bits (142), Expect = 1.499e-8 Identity = 27/50 (54.00%), Postives = 31/50 (62.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P IS PT H HV FD TG+F G+P +W L SNISK EQK NP+
Sbjct: 81 PNISYPTNFEHTVHVGFDAVTGEFTGMPEAWARLLMNSNISKQEQKKNPQ 130
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Fly
Match: Pak (gene:FBgn0267698 transcript:FBtr0334543) HSP 1 Score: 349.747 bits (896), Expect = 5.306e-113 Identity = 161/229 (70.31%), Postives = 189/229 (82.53%), Query Frame = 2
Query: 986 RHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+ S E + KL+ +VS DP KY +++IGQGASG V + T +VAIKQMNL QQPKK+LI+NEILV++ NKH N+VNYLDS++++ ELWVVMEYL GGSLTDVVTE CMDE QIA +CREV AL FLH+N VIHRDIKSDNILLGLDGSVK+ DFGFCAQ+ ++ R+TMVGTPYWMAPEVVTRKQYGPKVDLWSLGIM IEM+EGEPPYLNENPL+AL+
Sbjct: 542 KKMSDEEILEKLRTIVSVGDPNRKYTKMEKIGQGASGTVYTAIESSTGMEVAIKQMNLSQQPKKELIINEILVMRENKHPNVVNYLDSYLVSEELWVVMEYLPGGSLTDVVTETCMDEGQIAAVCREVLQALEFLHANQVIHRDIKSDNILLGLDGSVKLTDFGFCAQISPEQSKRTTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMAIEMVEGEPPYLNENPLKALY 770 HSP 2 Score: 79.337 bits (194), Expect = 5.306e-113 Identity = 40/74 (54.05%), Postives = 56/74 (75.68%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSE 1877
L ALYLIATNGKP +K+ +S F DFLD+CLEV++D+RASA LLKH F+ ++P++ L PLI+ A+E ++
Sbjct: 766 LKALYLIATNGKPEIKEKDKLSSAFQDFLDQCLEVEVDRRASALDLLKHPFL-KLARPLASLTPLIMAAKEATK 838 HSP 3 Score: 59.3066 bits (142), Expect = 1.499e-8 Identity = 27/50 (54.00%), Postives = 31/50 (62.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P IS PT H HV FD TG+F G+P +W L SNISK EQK NP+
Sbjct: 81 PNISYPTNFEHTVHVGFDAVTGEFTGMPEAWARLLMNSNISKQEQKKNPQ 130
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Fly
Match: Pak (gene:FBgn0267698 transcript:FBtr0334541) HSP 1 Score: 349.747 bits (896), Expect = 5.306e-113 Identity = 161/229 (70.31%), Postives = 189/229 (82.53%), Query Frame = 2
Query: 986 RHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+ S E + KL+ +VS DP KY +++IGQGASG V + T +VAIKQMNL QQPKK+LI+NEILV++ NKH N+VNYLDS++++ ELWVVMEYL GGSLTDVVTE CMDE QIA +CREV AL FLH+N VIHRDIKSDNILLGLDGSVK+ DFGFCAQ+ ++ R+TMVGTPYWMAPEVVTRKQYGPKVDLWSLGIM IEM+EGEPPYLNENPL+AL+
Sbjct: 542 KKMSDEEILEKLRTIVSVGDPNRKYTKMEKIGQGASGTVYTAIESSTGMEVAIKQMNLSQQPKKELIINEILVMRENKHPNVVNYLDSYLVSEELWVVMEYLPGGSLTDVVTETCMDEGQIAAVCREVLQALEFLHANQVIHRDIKSDNILLGLDGSVKLTDFGFCAQISPEQSKRTTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMAIEMVEGEPPYLNENPLKALY 770 HSP 2 Score: 79.337 bits (194), Expect = 5.306e-113 Identity = 40/74 (54.05%), Postives = 56/74 (75.68%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSE 1877
L ALYLIATNGKP +K+ +S F DFLD+CLEV++D+RASA LLKH F+ ++P++ L PLI+ A+E ++
Sbjct: 766 LKALYLIATNGKPEIKEKDKLSSAFQDFLDQCLEVEVDRRASALDLLKHPFL-KLARPLASLTPLIMAAKEATK 838 HSP 3 Score: 59.3066 bits (142), Expect = 1.499e-8 Identity = 27/50 (54.00%), Postives = 31/50 (62.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P IS PT H HV FD TG+F G+P +W L SNISK EQK NP+
Sbjct: 81 PNISYPTNFEHTVHVGFDAVTGEFTGMPEAWARLLMNSNISKQEQKKNPQ 130
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Fly
Match: Pak (gene:FBgn0267698 transcript:FBtr0334539) HSP 1 Score: 347.821 bits (891), Expect = 1.261e-112 Identity = 161/229 (70.31%), Postives = 189/229 (82.53%), Query Frame = 2
Query: 986 RHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+ S E + KL+ +VS DP KY +++IGQGASG V + T +VAIKQMNL QQPKK+LI+NEILV++ NKH N+VNYLDS++++ ELWVVMEYL GGSLTDVVTE CMDE QIA +CREV AL FLH+N VIHRDIKSDNILLGLDGSVK+ DFGFCAQ+ ++ R+TMVGTPYWMAPEVVTRKQYGPKVDLWSLGIM IEM+EGEPPYLNENPL+AL+
Sbjct: 406 KKMSDEEILEKLRTIVSVGDPNRKYTKMEKIGQGASGTVYTAIESSTGMEVAIKQMNLSQQPKKELIINEILVMRENKHPNVVNYLDSYLVSEELWVVMEYLPGGSLTDVVTETCMDEGQIAAVCREVLQALEFLHANQVIHRDIKSDNILLGLDGSVKLTDFGFCAQISPEQSKRTTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMAIEMVEGEPPYLNENPLKALY 634 HSP 2 Score: 80.1073 bits (196), Expect = 1.261e-112 Identity = 40/74 (54.05%), Postives = 56/74 (75.68%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSE 1877
L ALYLIATNGKP +K+ +S F DFLD+CLEV++D+RASA LLKH F+ ++P++ L PLI+ A+E ++
Sbjct: 630 LKALYLIATNGKPEIKEKDKLSSAFQDFLDQCLEVEVDRRASALDLLKHPFLK-LARPLASLTPLIMAAKEATK 702 HSP 3 Score: 59.6918 bits (143), Expect = 9.515e-9 Identity = 27/50 (54.00%), Postives = 31/50 (62.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P IS PT H HV FD TG+F G+P +W L SNISK EQK NP+
Sbjct: 81 PNISYPTNFEHTVHVGFDAVTGEFTGMPEAWARLLMNSNISKQEQKKNPQ 130
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Zebrafish
Match: pak2a (p21 protein (Cdc42/Rac)-activated kinase 2a [Source:ZFIN;Acc:ZDB-GENE-021011-2]) HSP 1 Score: 335.109 bits (858), Expect = 1.052e-109 Identity = 158/239 (66.11%), Postives = 191/239 (79.92%), Query Frame = 2
Query: 974 RRADRHCSG------EFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+ AD+ G E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQ+NL++QPKK+LI+NEILV+K K+ NIVN+LDSF++ +EL+VVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLH+N VIHRDIKSDN+LLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 209 KAADKQKKGKGKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVYTAIDVATGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSFLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 447 HSP 2 Score: 83.5741 bits (205), Expect = 1.052e-109 Identity = 40/71 (56.34%), Postives = 53/71 (74.65%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
L ALYLIATNG P L++P +SP+F DFL+RCLE+D++KR LL+H F+ +KP+S L PLIL A+E
Sbjct: 443 LRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGGGKELLQHPFLK-LAKPLSSLTPLILAAKE 512 HSP 3 Score: 61.2326 bits (147), Expect = 3.601e-9 Identity = 39/112 (34.82%), Postives = 56/112 (50.00%), Query Frame = 2
Query: 83 PPAPPIRSSSTLRRKDRYSVSXXXXXXXXXXXXXXXXXXXXXXXF-------------NYNDPKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
PPAPP+R SST + S+S S P+ ++K + + P+IS+P+ H HV FD TG+F G+P W L+ SNI+K+EQK NP+
Sbjct: 11 PPAPPVRMSSTFSTGIKDSMSTNPSSKPLPSVPEEKRDKPRNKIISIFSAEKGRKKDKDKERPEISNPSDFEHTIHVGFDSVTGEFTGMPEQWARLLQTSNITKSEQKKNPQ 122
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Zebrafish
Match: pak2a (p21 protein (Cdc42/Rac)-activated kinase 2a [Source:ZFIN;Acc:ZDB-GENE-021011-2]) HSP 1 Score: 335.109 bits (858), Expect = 1.172e-109 Identity = 158/239 (66.11%), Postives = 191/239 (79.92%), Query Frame = 2
Query: 974 RRADRHCSG------EFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+ AD+ G E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQ+NL++QPKK+LI+NEILV+K K+ NIVN+LDSF++ +EL+VVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLH+N VIHRDIKSDN+LLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 208 KAADKQKKGKGKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVYTAIDVATGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSFLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 446 HSP 2 Score: 83.5741 bits (205), Expect = 1.172e-109 Identity = 40/71 (56.34%), Postives = 53/71 (74.65%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
L ALYLIATNG P L++P +SP+F DFL+RCLE+D++KR LL+H F+ +KP+S L PLIL A+E
Sbjct: 442 LRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGGGKELLQHPFLK-LAKPLSSLTPLILAAKE 511 HSP 3 Score: 61.2326 bits (147), Expect = 3.657e-9 Identity = 39/112 (34.82%), Postives = 56/112 (50.00%), Query Frame = 2
Query: 83 PPAPPIRSSSTLRRKDRYSVSXXXXXXXXXXXXXXXXXXXXXXXF-------------NYNDPKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
PPAPP+R SST + S+S S P+ ++K + + P+IS+P+ H HV FD TG+F G+P W L+ SNI+K+EQK NP+
Sbjct: 11 PPAPPVRMSSTFSTGIKDSMSTNPSSKPLPSVPEEKRDKPRNKIISIFSAEKGRKKDKDKERPEISNPSDFEHTIHVGFDSVTGEFTGMPEQWARLLQTSNITKSEQKKNPQ 122
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Zebrafish
Match: pak2a (p21 protein (Cdc42/Rac)-activated kinase 2a [Source:ZFIN;Acc:ZDB-GENE-021011-2]) HSP 1 Score: 335.109 bits (858), Expect = 1.172e-109 Identity = 158/239 (66.11%), Postives = 191/239 (79.92%), Query Frame = 2
Query: 974 RRADRHCSG------EFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+ AD+ G E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQ+NL++QPKK+LI+NEILV+K K+ NIVN+LDSF++ +EL+VVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLH+N VIHRDIKSDN+LLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 208 KAADKQKKGKGKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVYTAIDVATGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSFLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 446 HSP 2 Score: 83.5741 bits (205), Expect = 1.172e-109 Identity = 40/71 (56.34%), Postives = 53/71 (74.65%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
L ALYLIATNG P L++P +SP+F DFL+RCLE+D++KR LL+H F+ +KP+S L PLIL A+E
Sbjct: 442 LRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGGGKELLQHPFLK-LAKPLSSLTPLILAAKE 511 HSP 3 Score: 61.2326 bits (147), Expect = 3.657e-9 Identity = 39/112 (34.82%), Postives = 56/112 (50.00%), Query Frame = 2
Query: 83 PPAPPIRSSSTLRRKDRYSVSXXXXXXXXXXXXXXXXXXXXXXXF-------------NYNDPKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
PPAPP+R SST + S+S S P+ ++K + + P+IS+P+ H HV FD TG+F G+P W L+ SNI+K+EQK NP+
Sbjct: 11 PPAPPVRMSSTFSTGIKDSMSTNPSSKPLPSVPEEKRDKPRNKIISIFSAEKGRKKDKDKERPEISNPSDFEHTIHVGFDSVTGEFTGMPEQWARLLQTSNITKSEQKKNPQ 122
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Zebrafish
Match: pak2a (p21 protein (Cdc42/Rac)-activated kinase 2a [Source:ZFIN;Acc:ZDB-GENE-021011-2]) HSP 1 Score: 335.109 bits (858), Expect = 1.172e-109 Identity = 158/239 (66.11%), Postives = 191/239 (79.92%), Query Frame = 2
Query: 974 RRADRHCSG------EFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+ AD+ G E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQ+NL++QPKK+LI+NEILV+K K+ NIVN+LDSF++ +EL+VVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLH+N VIHRDIKSDN+LLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 208 KAADKQKKGKGKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVYTAIDVATGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSFLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 446 HSP 2 Score: 83.5741 bits (205), Expect = 1.172e-109 Identity = 40/71 (56.34%), Postives = 53/71 (74.65%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
L ALYLIATNG P L++P +SP+F DFL+RCLE+D++KR LL+H F+ +KP+S L PLIL A+E
Sbjct: 442 LRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGGGKELLQHPFLK-LAKPLSSLTPLILAAKE 511 HSP 3 Score: 61.2326 bits (147), Expect = 3.657e-9 Identity = 39/112 (34.82%), Postives = 56/112 (50.00%), Query Frame = 2
Query: 83 PPAPPIRSSSTLRRKDRYSVSXXXXXXXXXXXXXXXXXXXXXXXF-------------NYNDPKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
PPAPP+R SST + S+S S P+ ++K + + P+IS+P+ H HV FD TG+F G+P W L+ SNI+K+EQK NP+
Sbjct: 11 PPAPPVRMSSTFSTGIKDSMSTNPSSKPLPSVPEEKRDKPRNKIISIFSAEKGRKKDKDKERPEISNPSDFEHTIHVGFDSVTGEFTGMPEQWARLLQTSNITKSEQKKNPQ 122
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Zebrafish
Match: pak1 (p21 protein (Cdc42/Rac)-activated kinase 1 [Source:ZFIN;Acc:ZDB-GENE-030826-29]) HSP 1 Score: 348.206 bits (892), Expect = 4.396e-109 Identity = 160/229 (69.87%), Postives = 188/229 (82.10%), Query Frame = 2
Query: 986 RHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+ S E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLH N VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 280 KKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAIDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHCNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALY 508 HSP 2 Score: 83.9593 bits (206), Expect = 3.537e-16 Identity = 40/80 (50.00%), Postives = 60/80 (75.00%), Query Frame = 3
Query: 1638 HISMKILCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSE 1877
+++ L ALYLIATNG P L++P +S +F DFL+RCLE+D++KR SA LL+H+F+ +KP+S L PLI+ A+E ++
Sbjct: 498 YLNENPLRALYLIATNGTPELQNPEKLSAVFRDFLNRCLEMDVEKRGSAKELLQHQFLK-VAKPLSSLTPLIIAAKEAAK 576 HSP 3 Score: 58.5362 bits (140), Expect = 3.026e-8 Identity = 25/50 (50.00%), Postives = 32/50 (64.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K EQK NP+
Sbjct: 72 PEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKLEQKKNPQ 121
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Xenopus
Match: flii (FLII actin remodeling protein [Source:Xenbase;Acc:XB-GENE-478681]) HSP 1 Score: 352.058 bits (902), Expect = 2.132e-114 Identity = 164/246 (66.67%), Postives = 195/246 (79.27%), Query Frame = 2
Query: 935 NQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
N S + R+ +R + E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 232 NSSTSTLYRNTDRQRKKSKMTDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAIDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 477 HSP 2 Score: 82.8037 bits (203), Expect = 2.132e-114 Identity = 40/71 (56.34%), Postives = 54/71 (76.06%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
L ALYLIATNG P L++P +S +F DFL+RCLE+D+D+R SA LL+H F+ +KP+S L PLI+ A+E
Sbjct: 473 LRALYLIATNGTPELQNPERLSAIFRDFLNRCLEMDVDRRGSAKELLQHPFLK-LAKPLSSLTPLIIAAKE 542 HSP 3 Score: 58.5362 bits (140), Expect = 4.085e-8 Identity = 25/50 (50.00%), Postives = 32/50 (64.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K EQK NP+
Sbjct: 68 PEISLPSDFEHTIHVGFDAVTGEFTGIPEQWARLLQTSNITKLEQKKNPQ 117
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Xenopus
Match: flii (FLII actin remodeling protein [Source:Xenbase;Acc:XB-GENE-478681]) HSP 1 Score: 351.288 bits (900), Expect = 3.662e-114 Identity = 164/246 (66.67%), Postives = 195/246 (79.27%), Query Frame = 2
Query: 935 NQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
N S + R+ +R + E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 180 NSSTSTLYRNTDRQRKKSKMTDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAIDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 425 HSP 2 Score: 83.1889 bits (204), Expect = 3.662e-114 Identity = 40/71 (56.34%), Postives = 54/71 (76.06%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
L ALYLIATNG P L++P +S +F DFL+RCLE+D+D+R SA LL+H F+ +KP+S L PLI+ A+E
Sbjct: 421 LRALYLIATNGTPELQNPERLSAIFRDFLNRCLEMDVDRRGSAKELLQHPFL-KLAKPLSSLTPLIIAAKE 490 HSP 3 Score: 58.5362 bits (140), Expect = 3.842e-8 Identity = 25/50 (50.00%), Postives = 32/50 (64.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K EQK NP+
Sbjct: 16 PEISLPSDFEHTIHVGFDAVTGEFTGIPEQWARLLQTSNITKLEQKKNPQ 65
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Xenopus
Match: qsox2 (quiescin sulfhydryl oxidase 2 [Source:Xenbase;Acc:XB-GENE-986398]) HSP 1 Score: 343.199 bits (879), Expect = 5.199e-111 Identity = 159/224 (70.98%), Postives = 185/224 (82.59%), Query Frame = 2
Query: 1001 EFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NKH NIVNYLDS+++ ELWVVMEYL GGSLTDVVTE CM E +IA +CRE AL FLHSN VIHRDIKSDNILLG+DG+VK+ DFGFCAQ+ +N RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 146 EEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYIAVDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKHPNIVNYLDSYLVGEELWVVMEYLAGGSLTDVVTETCMQEGEIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGAVKLTDFGFCAQISPEQNKRSTMVGTPYWMAPEVVTRKAYGPKVDVWSLGIMAIEMVEGEPPYLNENPLRALY 369 HSP 2 Score: 80.4925 bits (197), Expect = 5.199e-111 Identity = 38/71 (53.52%), Postives = 50/71 (70.42%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
L ALYLIATNG P L++P SP F DFL RCLE+D++KR SA LL+H F+ +KP+S L P I+ ++
Sbjct: 365 LRALYLIATNGTPQLQEPEKKSPAFRDFLSRCLEMDVEKRGSARDLLQHPFL-KVAKPMSSLTPYIITGKQ 434
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Xenopus
Match: flii (FLII actin remodeling protein [Source:Xenbase;Acc:XB-GENE-478681]) HSP 1 Score: 351.288 bits (900), Expect = 2.169e-110 Identity = 164/246 (66.67%), Postives = 195/246 (79.27%), Query Frame = 2
Query: 935 NQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
N S + R+ +R + E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 247 NSSTSTLYRNTDRQRKKSKMTDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAIDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 492 HSP 2 Score: 82.8037 bits (203), Expect = 8.983e-16 Identity = 40/77 (51.95%), Postives = 57/77 (74.03%), Query Frame = 3
Query: 1638 HISMKILCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
+++ L ALYLIATNG P L++P +S +F DFL+RCLE+D+D+R SA LL+H F+ +KP+S L PLI+ A+E
Sbjct: 482 YLNENPLRALYLIATNGTPELQNPERLSAIFRDFLNRCLEMDVDRRGSAKELLQHPFLK-LAKPLSSLTPLIIAAKE 557
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Xenopus
Match: taf3 (TATA-box binding protein associated factor 3 [Source:Xenbase;Acc:XB-GENE-940280]) HSP 1 Score: 336.65 bits (862), Expect = 1.596e-109 Identity = 156/226 (69.03%), Postives = 188/226 (83.19%), Query Frame = 2
Query: 995 SGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
S E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQ+NL++QPKK+LI+NEILV+K K+ NIVN+LDSF++++EL+VVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLH+N VIHRDIKSDN+LLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 222 SDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVFTAIDVATGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSFLVSDELYVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 447 HSP 2 Score: 82.0333 bits (201), Expect = 1.596e-109 Identity = 41/71 (57.75%), Postives = 53/71 (74.65%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
L ALYLIATNG P L+ P +SP+F DFL+R LE+D++KR SA LL+H F+ +KP+S L PLIL A+E
Sbjct: 443 LRALYLIATNGTPELQKPEKLSPIFRDFLNRSLEMDVEKRGSARELLQHPFLK-LAKPLSSLTPLILAAKE 512 HSP 3 Score: 58.9214 bits (141), Expect = 2.389e-8 Identity = 25/50 (50.00%), Postives = 32/50 (64.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K EQK NP+
Sbjct: 66 PEISPPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKLEQKKNPQ 115
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Mouse
Match: Pak3 (p21 (RAC1) activated kinase 3 [Source:MGI Symbol;Acc:MGI:1339656]) HSP 1 Score: 350.903 bits (899), Expect = 3.163e-114 Identity = 162/238 (68.07%), Postives = 192/238 (80.67%), Query Frame = 2
Query: 959 RHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
R+ +R + E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 235 RNTDRQRKKSKMTDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTALDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 472 HSP 2 Score: 82.8037 bits (203), Expect = 3.163e-114 Identity = 40/71 (56.34%), Postives = 54/71 (76.06%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
L ALYLIATNG P L++P +S +F DFL+RCLE+D+D+R SA LL+H F+ +KP+S L PLI+ A+E
Sbjct: 468 LRALYLIATNGTPELQNPERLSAVFRDFLNRCLEMDVDRRGSAKELLQHPFLK-LAKPLSSLTPLIIAAKE 537 HSP 3 Score: 58.5362 bits (140), Expect = 3.127e-8 Identity = 25/50 (50.00%), Postives = 32/50 (64.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K EQK NP+
Sbjct: 68 PEISLPSDFEHTIHVGFDAVTGEFTGIPEQWARLLQTSNITKLEQKKNPQ 117
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Mouse
Match: Pak3 (p21 (RAC1) activated kinase 3 [Source:MGI Symbol;Acc:MGI:1339656]) HSP 1 Score: 350.903 bits (899), Expect = 3.163e-114 Identity = 162/238 (68.07%), Postives = 192/238 (80.67%), Query Frame = 2
Query: 959 RHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
R+ +R + E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 235 RNTDRQRKKSKMTDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTALDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 472 HSP 2 Score: 82.8037 bits (203), Expect = 3.163e-114 Identity = 40/71 (56.34%), Postives = 54/71 (76.06%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
L ALYLIATNG P L++P +S +F DFL+RCLE+D+D+R SA LL+H F+ +KP+S L PLI+ A+E
Sbjct: 468 LRALYLIATNGTPELQNPERLSAVFRDFLNRCLEMDVDRRGSAKELLQHPFLK-LAKPLSSLTPLIIAAKE 537 HSP 3 Score: 58.5362 bits (140), Expect = 3.127e-8 Identity = 25/50 (50.00%), Postives = 32/50 (64.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K EQK NP+
Sbjct: 68 PEISLPSDFEHTIHVGFDAVTGEFTGIPEQWARLLQTSNITKLEQKKNPQ 117
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Mouse
Match: Pak3 (p21 (RAC1) activated kinase 3 [Source:MGI Symbol;Acc:MGI:1339656]) HSP 1 Score: 350.903 bits (899), Expect = 3.163e-114 Identity = 162/238 (68.07%), Postives = 192/238 (80.67%), Query Frame = 2
Query: 959 RHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
R+ +R + E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 235 RNTDRQRKKSKMTDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTALDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 472 HSP 2 Score: 82.8037 bits (203), Expect = 3.163e-114 Identity = 40/71 (56.34%), Postives = 54/71 (76.06%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
L ALYLIATNG P L++P +S +F DFL+RCLE+D+D+R SA LL+H F+ +KP+S L PLI+ A+E
Sbjct: 468 LRALYLIATNGTPELQNPERLSAVFRDFLNRCLEMDVDRRGSAKELLQHPFLK-LAKPLSSLTPLIIAAKE 537 HSP 3 Score: 58.5362 bits (140), Expect = 3.127e-8 Identity = 25/50 (50.00%), Postives = 32/50 (64.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K EQK NP+
Sbjct: 68 PEISLPSDFEHTIHVGFDAVTGEFTGIPEQWARLLQTSNITKLEQKKNPQ 117
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Mouse
Match: Pak3 (p21 (RAC1) activated kinase 3 [Source:MGI Symbol;Acc:MGI:1339656]) HSP 1 Score: 350.903 bits (899), Expect = 3.163e-114 Identity = 162/238 (68.07%), Postives = 192/238 (80.67%), Query Frame = 2
Query: 959 RHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
R+ +R + E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 235 RNTDRQRKKSKMTDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTALDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 472 HSP 2 Score: 82.8037 bits (203), Expect = 3.163e-114 Identity = 40/71 (56.34%), Postives = 54/71 (76.06%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
L ALYLIATNG P L++P +S +F DFL+RCLE+D+D+R SA LL+H F+ +KP+S L PLI+ A+E
Sbjct: 468 LRALYLIATNGTPELQNPERLSAVFRDFLNRCLEMDVDRRGSAKELLQHPFLK-LAKPLSSLTPLIIAAKE 537 HSP 3 Score: 58.5362 bits (140), Expect = 3.127e-8 Identity = 25/50 (50.00%), Postives = 32/50 (64.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K EQK NP+
Sbjct: 68 PEISLPSDFEHTIHVGFDAVTGEFTGIPEQWARLLQTSNITKLEQKKNPQ 117
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Mouse
Match: Pak1 (p21 (RAC1) activated kinase 1 [Source:MGI Symbol;Acc:MGI:1339975]) HSP 1 Score: 353.214 bits (905), Expect = 2.064e-111 Identity = 169/278 (60.79%), Postives = 205/278 (73.74%), Query Frame = 2
Query: 839 SPLLEVETDELGETGDSVTPCDKSKDSVSIEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+P +V T + T ++ TP D R+ + ++ S E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 211 TPTRDVATSPISPTENNTTPPDA---------------LTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALY 473 HSP 2 Score: 83.1889 bits (204), Expect = 5.932e-16 Identity = 40/80 (50.00%), Postives = 59/80 (73.75%), Query Frame = 3
Query: 1638 HISMKILCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSE 1877
+++ L ALYLIATNG P L++P +S +F DFL+RCLE+D++KR SA LL+H+F+ +KP+S L PLI A+E ++
Sbjct: 463 YLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFL-KIAKPLSSLTPLIAAAKEATK 541 HSP 3 Score: 58.9214 bits (141), Expect = 1.930e-8 Identity = 25/50 (50.00%), Postives = 33/50 (66.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K+EQK NP+
Sbjct: 73 PEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQ 122
BLAST of Non-specific serine/threonine protein kinase vs. UniProt/SwissProt
Match: sp|P35465|PAK1_RAT (Serine/threonine-protein kinase PAK 1 OS=Rattus norvegicus OX=10116 GN=Pak1 PE=1 SV=3) HSP 1 Score: 352.829 bits (904), Expect = 1.861e-110 Identity = 169/278 (60.79%), Postives = 205/278 (73.74%), Query Frame = 2
Query: 839 SPLLEVETDELGETGDSVTPCDKSKDSVSIEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+P +V T + T ++ TP D R+ + ++ S E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 211 TPTRDVATSPISPTENNTTPPDA---------------LTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALY 473 HSP 2 Score: 83.1889 bits (204), Expect = 4.259e-15 Identity = 40/80 (50.00%), Postives = 59/80 (73.75%), Query Frame = 3
Query: 1638 HISMKILCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSE 1877
+++ L ALYLIATNG P L++P +S +F DFL+RCLE+D++KR SA LL+H+F+ +KP+S L PLI A+E ++
Sbjct: 463 YLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFL-KIAKPLSSLTPLIAAAKEATK 541 HSP 3 Score: 59.3066 bits (142), Expect = 9.811e-8 Identity = 40/124 (32.26%), Postives = 59/124 (47.58%), Query Frame = 2
Query: 83 PPAPPIRSSSTL-------------------------RRKDRYSVSXXXXXXXXXXXXXXXXXXXXXXXFNYNDPKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
PPAPP+R++ST+ ++KDR+ S KK++K++ P+IS P+ H HV FD TG+F G+P W L+ SNI+K+EQK NP+
Sbjct: 12 PPAPPMRNTSTMIGAGSKDPGTLNHGSKPLPPNPEEKKKKDRFYRSILAGDKTNKKKEKER-------------PEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQ 122
BLAST of Non-specific serine/threonine protein kinase vs. UniProt/SwissProt
Match: sp|Q13153|PAK1_HUMAN (Serine/threonine-protein kinase PAK 1 OS=Homo sapiens OX=9606 GN=PAK1 PE=1 SV=2) HSP 1 Score: 352.829 bits (904), Expect = 1.937e-110 Identity = 169/278 (60.79%), Postives = 205/278 (73.74%), Query Frame = 2
Query: 839 SPLLEVETDELGETGDSVTPCDKSKDSVSIEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+P +V T + T ++ TP D R+ + ++ S E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 212 TPTRDVATSPISPTENNTTPPDA---------------LTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALY 474 HSP 2 Score: 82.8037 bits (203), Expect = 4.386e-15 Identity = 40/80 (50.00%), Postives = 59/80 (73.75%), Query Frame = 3
Query: 1638 HISMKILCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSE 1877
+++ L ALYLIATNG P L++P +S +F DFL+RCLE+D++KR SA LL+H+F+ +KP+S L PLI A+E ++
Sbjct: 464 YLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFLK-IAKPLSSLTPLIAAAKEATK 542 HSP 3 Score: 58.9214 bits (141), Expect = 1.328e-7 Identity = 25/50 (50.00%), Postives = 33/50 (66.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K+EQK NP+
Sbjct: 73 PEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQ 122
BLAST of Non-specific serine/threonine protein kinase vs. UniProt/SwissProt
Match: sp|O88643|PAK1_MOUSE (Serine/threonine-protein kinase PAK 1 OS=Mus musculus OX=10090 GN=Pak1 PE=1 SV=1) HSP 1 Score: 352.443 bits (903), Expect = 2.470e-110 Identity = 169/278 (60.79%), Postives = 205/278 (73.74%), Query Frame = 2
Query: 839 SPLLEVETDELGETGDSVTPCDKSKDSVSIEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+P +V T + T ++ TP D R+ + ++ S E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 212 TPTRDVATSPISPTENNTTPPDA---------------LTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTPFEKIGQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALY 474 HSP 2 Score: 75.8702 bits (185), Expect = 7.414e-13 Identity = 38/80 (47.50%), Postives = 57/80 (71.25%), Query Frame = 3
Query: 1638 HISMKILCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSE 1877
+++ L ALYLIATNG P L++P +S +F DFL CLE+D++KR SA LL+H+F+ +KP+S L PL+ A+E ++
Sbjct: 464 YLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLQCCLEMDVEKRGSAKELLQHQFL-KIAKPLSSLTPLMHAAKEATK 542 HSP 3 Score: 58.9214 bits (141), Expect = 1.461e-7 Identity = 25/50 (50.00%), Postives = 33/50 (66.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K+EQK NP+
Sbjct: 73 PEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQ 122
BLAST of Non-specific serine/threonine protein kinase vs. UniProt/SwissProt
Match: sp|Q08E52|PAK1_BOVIN (Serine/threonine-protein kinase PAK 1 OS=Bos taurus OX=9913 GN=PAK1 PE=1 SV=1) HSP 1 Score: 352.058 bits (902), Expect = 3.362e-110 Identity = 169/278 (60.79%), Postives = 205/278 (73.74%), Query Frame = 2
Query: 839 SPLLEVETDELGETGDSVTPCDKSKDSVSIEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+P +V T + T ++ TP D R+ + ++ S E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 211 TPTRDVATSPISPTENNTTPPDA---------------LTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALY 473 HSP 2 Score: 83.1889 bits (204), Expect = 4.222e-15 Identity = 40/80 (50.00%), Postives = 59/80 (73.75%), Query Frame = 3
Query: 1638 HISMKILCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSE 1877
+++ L ALYLIATNG P L++P +S +F DFL+RCLE+D++KR SA LL+H+F+ +KP+S L PLI A+E ++
Sbjct: 463 YLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFLK-IAKPLSSLTPLIAAAKEATK 541 HSP 3 Score: 58.9214 bits (141), Expect = 1.338e-7 Identity = 25/50 (50.00%), Postives = 33/50 (66.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K+EQK NP+
Sbjct: 73 PEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQ 122
BLAST of Non-specific serine/threonine protein kinase vs. UniProt/SwissProt
Match: sp|Q62829|PAK3_RAT (Serine/threonine-protein kinase PAK 3 OS=Rattus norvegicus OX=10116 GN=Pak3 PE=1 SV=1) HSP 1 Score: 351.288 bits (900), Expect = 7.426e-110 Identity = 163/246 (66.26%), Postives = 195/246 (79.27%), Query Frame = 2
Query: 935 NQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
N + + R+ +R + E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 227 NANSSTLYRNTDRQRKKSKMTDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTALDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 472 HSP 2 Score: 83.9593 bits (206), Expect = 2.414e-15 Identity = 41/77 (53.25%), Postives = 57/77 (74.03%), Query Frame = 3
Query: 1638 HISMKILCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
+++ L ALYLIATNG P L++P +S +F DFL+RCLE+D+D+R SA LL+H F+ +KP+S L PLIL A+E
Sbjct: 462 YLNENPLRALYLIATNGTPELQNPERLSAVFRDFLNRCLEMDVDRRGSAKELLQHPFLK-LAKPLSSLTPLILAAKE 537 HSP 3 Score: 58.5362 bits (140), Expect = 2.187e-7 Identity = 25/50 (50.00%), Postives = 32/50 (64.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K EQK NP+
Sbjct: 68 PEISLPSDFEHTIHVGFDAVTGEFTGIPEQWARLLQTSNITKLEQKKNPQ 117
BLAST of Non-specific serine/threonine protein kinase vs. TrEMBL
Match: G7YL78 (p21-activated kinase 1 OS=Clonorchis sinensis OX=79923 GN=CLF_110869 PE=4 SV=1) HSP 1 Score: 348.206 bits (892), Expect = 1.045e-115 Identity = 170/280 (60.71%), Postives = 200/280 (71.43%), Query Frame = 2
Query: 896 PCDKSKDSVSIEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNN---------------------ELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
P D V+ + S R RH R D +LK VVS DPY KY +++++GQGASGVV G ++ TKK VAIKQMNL QQPKK+LI+NEILV+K+N+ NIVNYLDS++++ ELWVVMEYLDGGSLTDVV E CM+E IA IC+E+ +AL FLH+N VIHRDIKSDNILLG+DGSVK+ DFGFCAQL RSTMVGTPYWMAPEVV+RKQYGPKVD+WSLGIM IEML+GEPPYLNENPLRAL+
Sbjct: 553 PLDHHYPKVNPKAPTSATR-RRHGSQRITDTQV-----YERLKAVVSKGDPYQKYRLVEKVGQGASGVVYSGYEIGTKKLVAIKQMNLAQQPKKELIVNEILVMKSNRQANIVNYLDSYLVSTAVNRQPSTNGDNPSSASATGEELWVVMEYLDGGSLTDVVMETCMEEGHIAAICKEILLALEFLHANRVIHRDIKSDNILLGMDGSVKLTDFGFCAQLSADATKRSTMVGTPYWMAPEVVSRKQYGPKVDIWSLGIMAIEMLDGEPPYLNENPLRALY 826 HSP 2 Score: 100.908 bits (250), Expect = 1.045e-115 Identity = 48/72 (66.67%), Postives = 60/72 (83.33%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQ 1871
L ALYLIATNGKP +K+ +SP+FLDFLDRCLEVD+++RA+AS LLKH FI ++P+S L+PLI LAREQ
Sbjct: 822 LRALYLIATNGKPEIKERDRLSPVFLDFLDRCLEVDVEQRATASELLKHPFIAHCAEPLSSLVPLIQLAREQ 893
BLAST of Non-specific serine/threonine protein kinase vs. TrEMBL
Match: A0A4S2MCH0 (Protein kinase domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_002010 PE=4 SV=1) HSP 1 Score: 347.821 bits (891), Expect = 1.363e-115 Identity = 161/240 (67.08%), Postives = 189/240 (78.75%), Query Frame = 2
Query: 1016 KLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNN---------------------ELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+LK VVS DPY KY +++++GQGASGVV G ++ TKK VAIKQMNL QQPKK+LI+NEILV+K+N+ NIVNYLDS++++ ELWVVMEYLDGGSLTDVV E CM+E IA IC+E+ +AL FLH+N VIHRDIKSDNILLG+DGSVK+ DFGFCAQL RSTMVGTPYWMAPEVV+RKQYGPKVD+WSLGIM IEML+GEPPYLNENPLRAL+
Sbjct: 585 RLKAVVSKGDPYQKYRLVEKVGQGASGVVYSGYEIGTKKLVAIKQMNLAQQPKKELIVNEILVMKSNRQANIVNYLDSYLVSTTVNRQPSTNGDNPSSASGTGEELWVVMEYLDGGSLTDVVMETCMEEGHIAAICKEILLALEFLHANRVIHRDIKSDNILLGMDGSVKLTDFGFCAQLSADATKRSTMVGTPYWMAPEVVSRKQYGPKVDIWSLGIMAIEMLDGEPPYLNENPLRALY 824 HSP 2 Score: 100.908 bits (250), Expect = 1.363e-115 Identity = 48/72 (66.67%), Postives = 60/72 (83.33%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQ 1871
L ALYLIATNGKP +K+ +SP+FLDFLDRCLEVD+++RA+AS LLKH FI ++P+S L+PLI LAREQ
Sbjct: 820 LRALYLIATNGKPEIKERDRLSPVFLDFLDRCLEVDVEQRATASELLKHPFIAHCAEPLSSLVPLIQLAREQ 891
BLAST of Non-specific serine/threonine protein kinase vs. TrEMBL
Match: A0A419PPJ4 (Serine/threonine-protein kinase PAK 1 OS=Clonorchis sinensis OX=79923 GN=Pak1 PE=4 SV=1) HSP 1 Score: 347.821 bits (891), Expect = 1.981e-115 Identity = 161/240 (67.08%), Postives = 189/240 (78.75%), Query Frame = 2
Query: 1016 KLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNN---------------------ELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+LK VVS DPY KY +++++GQGASGVV G ++ TKK VAIKQMNL QQPKK+LI+NEILV+K+N+ NIVNYLDS++++ ELWVVMEYLDGGSLTDVV E CM+E IA IC+E+ +AL FLH+N VIHRDIKSDNILLG+DGSVK+ DFGFCAQL RSTMVGTPYWMAPEVV+RKQYGPKVD+WSLGIM IEML+GEPPYLNENPLRAL+
Sbjct: 745 RLKAVVSKGDPYQKYRLVEKVGQGASGVVYSGYEIGTKKLVAIKQMNLAQQPKKELIVNEILVMKSNRQANIVNYLDSYLVSTAVNRQPSTNGDNPSSASATGEELWVVMEYLDGGSLTDVVMETCMEEGHIAAICKEILLALEFLHANRVIHRDIKSDNILLGMDGSVKLTDFGFCAQLSADATKRSTMVGTPYWMAPEVVSRKQYGPKVDIWSLGIMAIEMLDGEPPYLNENPLRALY 984 HSP 2 Score: 100.523 bits (249), Expect = 1.981e-115 Identity = 48/72 (66.67%), Postives = 60/72 (83.33%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQ 1871
L ALYLIATNGKP +K+ +SP+FLDFLDRCLEVD+++RA+AS LLKH FI ++P+S L+PLI LAREQ
Sbjct: 980 LRALYLIATNGKPEIKERDRLSPVFLDFLDRCLEVDVEQRATASELLKHPFIAHCAEPLSSLVPLIQLAREQ 1051 HSP 3 Score: 70.0922 bits (170), Expect = 2.256e-8 Identity = 57/134 (42.54%), Postives = 83/134 (61.94%), Query Frame = 2
Query: 11 ILYGVFMDN---KSNSIEINETLDSELPPAPPIRSSSTLRRKDRYSV--SXXXXXXXXXXXXXXXXXXXXXXXFNY------NDPKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
++ G ++N S + +N++ SE PAPP+RSSSTLR+KD + SKPLP+ P K+EKKKK + + ++P+IS PT V+H+ HVVFD T +GLP +W+ L +SNIS+ EQ+ N E
Sbjct: 40 LMAGTNVNNLSPPSATFLLNDSNISEFAPAPPVRSSSTLRKKDGLVLPPSKPLPTLPGKEEKKKKKRGNLSRLLKFSRPEKKHEPQISGPTGVTHDLHVVFDRDTRQIKGLPEAWRLLLMSSNISEKEQQQNSE 173
BLAST of Non-specific serine/threonine protein kinase vs. TrEMBL
Match: A0A4S2M814 (Protein kinase domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_002010 PE=4 SV=1) HSP 1 Score: 347.436 bits (890), Expect = 2.340e-115 Identity = 161/240 (67.08%), Postives = 189/240 (78.75%), Query Frame = 2
Query: 1016 KLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNN---------------------ELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+LK VVS DPY KY +++++GQGASGVV G ++ TKK VAIKQMNL QQPKK+LI+NEILV+K+N+ NIVNYLDS++++ ELWVVMEYLDGGSLTDVV E CM+E IA IC+E+ +AL FLH+N VIHRDIKSDNILLG+DGSVK+ DFGFCAQL RSTMVGTPYWMAPEVV+RKQYGPKVD+WSLGIM IEML+GEPPYLNENPLRAL+
Sbjct: 690 RLKAVVSKGDPYQKYRLVEKVGQGASGVVYSGYEIGTKKLVAIKQMNLAQQPKKELIVNEILVMKSNRQANIVNYLDSYLVSTTVNRQPSTNGDNPSSASGTGEELWVVMEYLDGGSLTDVVMETCMEEGHIAAICKEILLALEFLHANRVIHRDIKSDNILLGMDGSVKLTDFGFCAQLSADATKRSTMVGTPYWMAPEVVSRKQYGPKVDIWSLGIMAIEMLDGEPPYLNENPLRALY 929 HSP 2 Score: 100.908 bits (250), Expect = 2.340e-115 Identity = 48/72 (66.67%), Postives = 60/72 (83.33%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQ 1871
L ALYLIATNGKP +K+ +SP+FLDFLDRCLEVD+++RA+AS LLKH FI ++P+S L+PLI LAREQ
Sbjct: 925 LRALYLIATNGKPEIKERDRLSPVFLDFLDRCLEVDVEQRATASELLKHPFIAHCAEPLSSLVPLIQLAREQ 996
BLAST of Non-specific serine/threonine protein kinase vs. TrEMBL
Match: A0A4E0RI63 (p21-activated kinase 1 OS=Fasciola hepatica OX=6192 GN=D915_001478 PE=4 SV=1) HSP 1 Score: 348.977 bits (894), Expect = 4.986e-115 Identity = 161/219 (73.52%), Postives = 186/219 (84.93%), Query Frame = 2
Query: 1016 KLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
KL+ +VS DP KY+ + IGQGASGVV GG ++ T ++VAIK+MNLKQQPKK+LI+NEI V+KA KH N+VNYLDS+++ +ELWVVMEYLDGGSLTDVVTE M+E QIA +CRE AL FLH N VIHRDIKSDNILLGLDGSVK+ DFGFCAQL + RSTMVGTPYWMAPEVVTRKQYGPKVD+WSLGIM IEML+GEPPYLNENP+RAL+
Sbjct: 685 KLRMIVSIGDPKRKYKRQEVIGQGASGVVYGGLEVATGRRVAIKEMNLKQQPKKELIVNEIYVMKAKKHPNVVNYLDSYLVGDELWVVMEYLDGGSLTDVVTETYMEEGQIAAVCRECLQALDFLHKNQVIHRDIKSDNILLGLDGSVKLTDFGFCAQLSSEQTKRSTMVGTPYWMAPEVVTRKQYGPKVDIWSLGIMAIEMLDGEPPYLNENPVRALY 903 HSP 2 Score: 97.8265 bits (242), Expect = 4.986e-115 Identity = 45/72 (62.50%), Postives = 58/72 (80.56%), Query Frame = 3
Query: 1662 ALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSE 1877
ALYLI TNGKP +K+ +SP F DFLDRCLEV +D+RASA LL+H FI ++KP+S L+PLIL+AREQ++
Sbjct: 901 ALYLIGTNGKPEIKEREKLSPEFQDFLDRCLEVKVDQRASAQELLQHPFIVKRAKPLSSLMPLILVAREQAK 972
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Cavefish
Match: PAK3 (p21 (RAC1) activated kinase 3 [Source:HGNC Symbol;Acc:HGNC:8592]) HSP 1 Score: 351.288 bits (900), Expect = 2.359e-111 Identity = 162/238 (68.07%), Postives = 190/238 (79.83%), Query Frame = 2
Query: 959 RHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
RH +R + E + +L+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ +N RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLR F
Sbjct: 243 RHTDRQRKKSKMTDEEILERLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAIDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQNKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRHSF 480 HSP 2 Score: 53.9138 bits (128), Expect = 5.565e-7 Identity = 43/119 (36.13%), Postives = 60/119 (50.42%), Query Frame = 2
Query: 41 SNSIEINETLDSELPPAPPIRSSSTLRRKDRYSVSXXXXXXXXXXXXXXXXXXXXXXXFNYND------PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
S+S++I E PPAPP+R SS+ R +S +P K +K + + N P+IS P+ H HV FD TG+F G+P W L+ SNI+K EQK NP+
Sbjct: 2 SDSVDIEEK-----PPAPPLRMSSSSRDSSLHSSKPLPMAPEEKNKKSARLRSIFPGGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTGIPEQWARLLQTSNITKLEQKKNPQ 115
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Cavefish
Match: PAK3 (p21 (RAC1) activated kinase 3 [Source:HGNC Symbol;Acc:HGNC:8592]) HSP 1 Score: 350.903 bits (899), Expect = 6.491e-111 Identity = 162/238 (68.07%), Postives = 190/238 (79.83%), Query Frame = 2
Query: 959 RHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
RH +R + E + +L+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ +N RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLR F
Sbjct: 264 RHTDRQRKKSKMTDEEILERLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAIDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQNKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRHSF 501 HSP 2 Score: 53.9138 bits (128), Expect = 6.183e-7 Identity = 43/119 (36.13%), Postives = 60/119 (50.42%), Query Frame = 2
Query: 41 SNSIEINETLDSELPPAPPIRSSSTLRRKDRYSVSXXXXXXXXXXXXXXXXXXXXXXXFNYND------PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
S+S++I E PPAPP+R SS+ R +S +P K +K + + N P+IS P+ H HV FD TG+F G+P W L+ SNI+K EQK NP+
Sbjct: 2 SDSVDIEEK-----PPAPPLRMSSSSRDSSLHSSKPLPMAPEEKNKKSARLRSIFPGGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTGIPEQWARLLQTSNITKLEQKKNPQ 115
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Cavefish
Match: PAK3 (p21 (RAC1) activated kinase 3 [Source:HGNC Symbol;Acc:HGNC:8592]) HSP 1 Score: 350.903 bits (899), Expect = 6.491e-111 Identity = 162/238 (68.07%), Postives = 190/238 (79.83%), Query Frame = 2
Query: 959 RHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
RH +R + E + +L+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ +N RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLR F
Sbjct: 264 RHTDRQRKKSKMTDEEILERLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAIDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQNKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRHSF 501 HSP 2 Score: 53.9138 bits (128), Expect = 6.183e-7 Identity = 43/119 (36.13%), Postives = 60/119 (50.42%), Query Frame = 2
Query: 41 SNSIEINETLDSELPPAPPIRSSSTLRRKDRYSVSXXXXXXXXXXXXXXXXXXXXXXXFNYND------PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
S+S++I E PPAPP+R SS+ R +S +P K +K + + N P+IS P+ H HV FD TG+F G+P W L+ SNI+K EQK NP+
Sbjct: 2 SDSVDIEEK-----PPAPPLRMSSSSRDSSLHSSKPLPMAPEEKNKKSARLRSIFPGGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTGIPEQWARLLQTSNITKLEQKKNPQ 115
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Cavefish
Match: pak2a (p21 protein (Cdc42/Rac)-activated kinase 2a [Source:ZFIN;Acc:ZDB-GENE-021011-2]) HSP 1 Score: 334.724 bits (857), Expect = 1.124e-103 Identity = 159/240 (66.25%), Postives = 190/240 (79.17%), Query Frame = 2
Query: 974 RRADRH-------CSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
R ADR + E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQ+NL++QPKK+LI+NEILV+K K+ NIVN+LDSF++ EL+VVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLH+N VIHRDIKSDN+LLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 302 RAADRQKKNPKGKMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVFTAIDVATGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSFLVGEELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 541 HSP 2 Score: 81.6481 bits (200), Expect = 1.463e-15 Identity = 39/77 (50.65%), Postives = 56/77 (72.73%), Query Frame = 3
Query: 1638 HISMKILCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
+++ L ALYLIATNG P L++P +SP+F DFL+RCLE+D++KR LL+H F+ +KP+S L PLIL A++
Sbjct: 531 YLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGGGKELLQHPFL-KLAKPLSSLTPLILAAKD 606 HSP 3 Score: 59.3066 bits (142), Expect = 1.205e-8 Identity = 25/50 (50.00%), Postives = 33/50 (66.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K+EQK NP+
Sbjct: 163 PEISPPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQ 212
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Cavefish
Match: pak2b (p21 protein (Cdc42/Rac)-activated kinase 2b [Source:ZFIN;Acc:ZDB-GENE-030131-3759]) HSP 1 Score: 330.487 bits (846), Expect = 1.402e-103 Identity = 155/224 (69.20%), Postives = 185/224 (82.59%), Query Frame = 2
Query: 1001 EFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQ+NL++QPKK+LI+NEILV+K K+ NIVN+LDSF++ EL+VVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLH+N VIHRDIKSDN+LLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 195 EEIMDKLRTIVSIGDPKKKYTRYEKIGQGASGTVYTAIDVATGQEVAIKQINLQKQPKKELIINEILVMKELKNPNIVNFLDSFLVGEELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 418 HSP 2 Score: 81.2629 bits (199), Expect = 1.579e-15 Identity = 40/77 (51.95%), Postives = 56/77 (72.73%), Query Frame = 3
Query: 1638 HISMKILCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
+++ L ALYLIATNG P L+ P +SP+F DFL RCLE+D++KR S+ LL+H F+ +KP+S L PLIL A++
Sbjct: 408 YLNENPLRALYLIATNGTPELQSPEKLSPIFRDFLARCLEMDVEKRGSSKELLQHPFLK-LAKPLSSLTPLILAAKD 483
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Sea Lamprey
Match: pak4 (p21 protein (Cdc42/Rac)-activated kinase 4 [Source:ZFIN;Acc:ZDB-GENE-040704-69]) HSP 1 Score: 260.381 bits (664), Expect = 5.310e-76 Identity = 116/225 (51.56%), Postives = 168/225 (74.67%), Query Frame = 2
Query: 995 SGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRAL 1669
S E F + L+ VV DP E +IG+G++GVV D + ++VA+K+M+L++Q +++L+ NE+++++ H+N+V+ +S+++ +ELWVVME+L+GG+LTD+VT M+E QIAT+C V AL +LH VIHRDIKSD+ILL DG +K+ DFGFCAQ+ R ++VGTPYWMAPEV++R YGP+VD+WSLGIMVIEM++GEPPY NE PL+A+
Sbjct: 398 SHEQFRAALQQVVDPGDPSEYLENFIKIGEGSTGVVCLAVDRRSGRQVAVKKMHLRKQQRRELLFNEVVIMREYHHENVVDMYNSYLVADELWVVMEFLEGGALTDIVTHTRMNEEQIATVCVSVLRALAYLHGQGVIHRDIKSDSILLTADGRIKLSDFGFCAQVSKEVTRRKSLVGTPYWMAPEVISRLPYGPEVDVWSLGIMVIEMVDGEPPYFNEPPLQAM 622
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Sea Lamprey
Match: stk25a (serine/threonine kinase 25a [Source:ZFIN;Acc:ZDB-GENE-041010-92]) HSP 1 Score: 178.333 bits (451), Expect = 7.504e-52 Identity = 92/216 (42.59%), Postives = 131/216 (60.65%), Query Frame = 2
Query: 1034 SSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILN-EILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIK--SDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
S +DP + +RIG+G+ G V G D TK+ VAIK ++L++ + + EI V+ + +Y S++ +LW++MEYL GGS D++ ++E QIATI RE+ L +LHS IHRDIK + N+LL G VK+ DFG QL + R+T VGTP+WMAPEV+ + Y K D+WSLGI IE+ +GEPP +P+R LF
Sbjct: 18 SCADPEELFTKQERIGKGSFGEVFKGIDNRTKEVVAIKIIDLEEAEDEIEDIQQEITVLSQCDSPYVTHYFGSYLKGTKLWIIMEYLGGGSALDLIKPGPLEEHQIATILREILKGLDYLHSERKIHRDIKGPAANVLLSEQGDVKLADFGVAGQLTDTQIKRNTFVGTPFWMAPEVIKQSAYDFKADIWSLGITAIELAKGEPPNSELHPMRVLF 233 HSP 2 Score: 44.669 bits (104), Expect = 7.504e-52 Identity = 26/63 (41.27%), Postives = 34/63 (53.97%), Query Frame = 3
Query: 1665 LYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLI 1853
L+LI N P+L+ S F +F+D CL D R +A LLKH+FI +K S L LI
Sbjct: 232 LFLIPKNNPPTLE--GNYSKAFKEFVDSCLNKDPQFRPTAKELLKHKFIQRSAKKTSYLTELI 292
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Sea Lamprey
Match: MAP4K3 (mitogen-activated protein kinase kinase kinase kinase 3 [Source:HGNC Symbol;Acc:HGNC:6865]) HSP 1 Score: 174.481 bits (441), Expect = 1.864e-48 Identity = 91/213 (42.72%), Postives = 131/213 (61.50%), Query Frame = 2
Query: 1058 YEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVV-TEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEV--VTRK-QYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALFNSNK 1684
+E+ +R+G G G V R+++T + VAIK + L+ +I EI+++K KH NIV Y S++ +++LW+ ME+ GGSL D+ + E QIA + RE L +LHS +HRDIK NILL DG VK+ DFG AQ+ R + +GTPYWMAPEV V RK Y + D+W++GI IE+ E +PP + +P+RALF +K
Sbjct: 14 FELAERLGSGTYGDVHKARNVQTGELVAIKIIKLEPGDDFSVIQQEIVMMKECKHTNIVAYYGSYLRHDKLWICMEHCGGGSLQDIYHVTGPLTERQIAYVTRETLQGLAYLHSEGKMHRDIKGANILLTDDGDVKLADFGVSAQITATIAKRKSFIGTPYWMAPEVAAVERKGGYNHQCDVWAVGITAIELAELQPPMFDLHPMRALFLMSK 226 HSP 2 Score: 37.3502 bits (85), Expect = 1.864e-48 Identity = 23/70 (32.86%), Postives = 38/70 (54.29%), Query Frame = 3
Query: 1662 ALYLIATNG--KPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAR 1865
AL+L++ +G P L+D SP+F +F+ L + KR +A +L H ++ +P+S L LL R
Sbjct: 220 ALFLMSKSGFQPPKLRDKQKWSPVFHNFIKMALTKNPKKRPTAEKMLTHSYV---QQPLSRAQALELLER 286
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Sea Lamprey
Match: slka (STE20-like kinase a [Source:ZFIN;Acc:ZDB-GENE-050411-66]) HSP 1 Score: 155.992 bits (393), Expect = 6.230e-45 Identity = 80/217 (36.87%), Postives = 126/217 (58.06%), Query Frame = 2
Query: 1043 DPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTE--ACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVV---TRKQ--YGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
DP + ++ +G GA G V ++ E+ A K + K + + + + EI ++ H NIV L++F N+LW+ +E+ GG++ ++ E + E QI +C+++ AL +LH++ +IHRD+K+ NILL +G VK+ DFG A+ R + +GTPYWMAPEVV T K Y K D+WSLGI +IE+ + EPP NP+R +
Sbjct: 28 DPAELWSVIGELGDGAFGKVYKAQNKESGVLAAAKVIETKSEEELEDYMVEIEILALCDHPNIVGLLNAFYYENKLWIFIEFCPGGAVDAIMLELERGLAEPQIRVVCKQMLEALNYLHTHRIIHRDLKAGNILLTFEGDVKLADFGVSAKNTRTLQRRDSFIGTPYWMAPEVVMCETMKDAPYDYKADIWSLGITLIELAQMEPPNHELNPMRVML 244 HSP 2 Score: 43.5134 bits (101), Expect = 6.230e-45 Identity = 26/59 (44.07%), Postives = 36/59 (61.02%), Query Frame = 3
Query: 1674 IATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFIT--DKSKPVSILI 1844
IA N PSL P+ S F DFL +CL+ + + R +A LL+H FI+ D +KP+ LI
Sbjct: 246 IAKNDPPSLSFPSKWSAEFTDFLKKCLDKNPETRWNALQLLQHSFISGVDSNKPLRELI 304
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Sea Lamprey
Match: myo3a (myosin IIIA [Source:ZFIN;Acc:ZDB-GENE-041026-4]) HSP 1 Score: 154.836 bits (390), Expect = 1.423e-44 Identity = 85/232 (36.64%), Postives = 132/232 (56.90%), Query Frame = 2
Query: 1043 DPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQM----NLK---QQPKKDLILNEILVIKANKHQNIVNYLDSF-----IMNNELWVVMEYLDGGSLTDVVTEAC-----MDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQ-----YGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
DP +E+++ IG+G G V + K A+K + NL+ Q +++ ++ +H NIV + + + ++LW+V+E +GGS+TD+V MD IA I R + L LHS+++IHRDIK +NILL DG ++++DFG AQ+ R R+T VGTP+WMAPEV+ +Q Y + D+WS+GI IE+ +G+PP +P+RALF
Sbjct: 19 DPSDTWELVETIGKGTYGKVYKATSRKDGSKAAVKTLDPVNNLETSNQDIDEEIEAEYSILTSLPEHPNIVRFFGMYYQKDGVNGDQLWIVLELCNGGSVTDLVKGQLKRGERMDVMLIAYILRGALLGLNHLHSHNIIHRDIKGNNILLTTDGGIRLVDFGVSAQVSKTRVERNTAVGTPFWMAPEVIACEQQLDYMYDRRCDVWSMGITAIELGDGDPPLYELHPMRALF 250 HSP 2 Score: 43.5134 bits (101), Expect = 1.423e-44 Identity = 22/52 (42.31%), Postives = 32/52 (61.54%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFI 1811
+ AL+ I N P+LK P PLF +F+ +CL D ++RA+ S LL+ FI
Sbjct: 246 MRALFKIPRNPPPTLKQPELWPPLFNEFIAQCLVKDFERRATVSQLLEDPFI 297
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Yeast
Match: STE20 (Cdc42p-activated signal transducing kinase; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p; member of the PAK (p21-activated kinase) family [Source:SGD;Acc:S000000999]) HSP 1 Score: 310.071 bits (793), Expect = 1.436e-97 Identity = 138/222 (62.16%), Postives = 174/222 (78.38%), Query Frame = 2
Query: 1007 FISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+KL + S DP +KY L +IGQGASG V ++ T VAIKQMNL++QPKK+LI+NEILV+K +KH NIVN++DS+++ +LWV+MEY++GGSLTDVVT + E QI +CRE L FLHS V+HRDIKSDNILL ++G +K+ DFGFCAQ+ R+TMVGTPYWMAPEVV+RK+YGPKVD+WSLGIM+IEM+EGEPPYLNE PLRAL+
Sbjct: 603 LYAKLNEICSDGDPSTKYANLVKIGQGASGGVYTAYEIGTNVSVAIKQMNLEKQPKKELIINEILVMKGSKHPNIVNFIDSYVLKGDLWVIMEYMEGGSLTDVVTHCILTEGQIGAVCRETLSGLEFLHSKGVLHRDIKSDNILLSMEGDIKLTDFGFCAQINELNLKRTTMVGTPYWMAPEVVSRKEYGPKVDIWSLGIMIIEMIEGEPPYLNETPLRALY 824 HSP 2 Score: 65.0846 bits (157), Expect = 1.436e-97 Identity = 37/70 (52.86%), Postives = 47/70 (67.14%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAR 1865
L ALYLIATNG P LK+P +S FLD CL V+ + RASA+ LL E+IT+ ++ S L PL+ LAR
Sbjct: 820 LRALYLIATNGTPKLKEPENLSSSLKKFLDWCLCVEPEDRASATELLHDEYITEIAEANSSLAPLVKLAR 889
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Yeast
Match: CLA4 (Cdc42p-activated signal transducing kinase; member of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a paralog, SKM1, that arose from the whole genome duplication [Source:SGD;Acc:S000005242]) HSP 1 Score: 260.766 bits (665), Expect = 3.114e-78 Identity = 127/250 (50.80%), Postives = 176/250 (70.40%), Query Frame = 2
Query: 1007 FISKLKGVVSSSDPYSKYEILDRIGQGASGVV----------------MGGRDLE---TKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFI-MNNELWVVMEYLDGGSLTDVV--------TEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+SKLK V ++DP ++++++ GQGASG V + D++ KVAIKQM L +QP+K+LI+NEILV+K ++H+NIVN+L++++ +++LWVVME+++GGSLTD++ + + + E QIA I RE L FLH H+IHRDIKSDN+LL VKI DFGFCA+L R+ R+TMVGTPYWMAPEVV +++Y K+D+WSLGIM IEMLEGEPPYLNE+PL+AL+
Sbjct: 529 IMSKLKKVTVNADPSQCFKVIEKAGQGASGSVYLAERTHIPTESNMIELINNDIDEPHVGDKVAIKQMVLSKQPRKELIVNEILVMKDSRHKNIVNFLEAYLRTDDDLWVVMEFMEGGSLTDIIENSPTNDNSHSPLTEPQIAYIVRETCQGLKFLHDKHIIHRDIKSDNVLLDTRARVKITDFGFCARLTDKRSKRATMVGTPYWMAPEVVKQREYDEKIDVWSLGIMTIEMLEGEPPYLNEDPLKALY 778 HSP 2 Score: 49.6766 bits (117), Expect = 3.114e-78 Identity = 28/53 (52.83%), Postives = 31/53 (58.49%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFIT 1814
L ALYLIATNG P LK P ++S FL CL VD+ RAS LL H F
Sbjct: 774 LKALYLIATNGTPKLKHPESLSLEIKRFLSVCLCVDVRYRASTEELLHHGFFN 826 HSP 3 Score: 49.2914 bits (116), Expect = 2.353e-6 Identity = 21/46 (45.65%), Postives = 30/46 (65.22%), Query Frame = 2
Query: 236 ISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDN 373
+SSPT +H HV FDP TG F G+P +W++ LK S I+ + +N
Sbjct: 184 VSSPTNFTHKVHVGFDPETGSFVGMPTNWEKLLKHSRITGEDWNNN 229
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Yeast
Match: SKM1 (Member of the PAK family of serine/threonine protein kinases; similar to Ste20p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth; SKM1 has a paralog, CLA4, that arose from the whole genome duplication [Source:SGD;Acc:S000005473]) HSP 1 Score: 245.743 bits (626), Expect = 6.066e-76 Identity = 119/250 (47.60%), Postives = 169/250 (67.60%), Query Frame = 2
Query: 1007 FISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLE-----------------TKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNE-LWVVMEYLDGGSLTDVVTEAC----------MDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
I K+K V +P ++++++ GQGASG V + ++ ++VAIKQ+ L +QPKK LI+NE+LV+ ++ +NIVN+L+++I+++E LWV+MEY++GG LTD++ ++E Q+A I +E L FLH+ +IHRDIKSDNILL G VKI DFGFC +L R+ R+TMVGTPYWMAPE+V +K Y KVD+WSLGIM+IEM+EGEPPYLNE+PL+AL+
Sbjct: 343 IIMKMKTVAIDVNPRPYFQLVEKAGQGASGAVYLSKRIKLPQENDPRFLKSHCHRVVGERVAIKQIRLSEQPKKQLIMNELLVMNDSRQENIVNFLEAYIIDDEELWVIMEYMEGGCLTDILDAVARSNTGEHSSPLNENQMAYIVKETCQGLKFLHNKKIIHRDIKSDNILLNSQGLVKITDFGFCVELTEKRSKRATMVGTPYWMAPEIVNQKGYDEKVDVWSLGIMLIEMIEGEPPYLNEDPLKALY 592 HSP 2 Score: 57.3806 bits (137), Expect = 6.066e-76 Identity = 29/65 (44.62%), Postives = 39/65 (60.00%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPL 1850
L ALYLIA NG P L+ P +VS FLD CL+V+++ RAS LL EF++ P + + L
Sbjct: 588 LKALYLIANNGSPKLRHPESVSKQTKQFLDACLQVNVESRASVRKLLTFEFLSMACSPEQLKVSL 652
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Yeast
Match: KIC1 (Protein kinase of the PAK/Ste20 family, required for cell integrity; physically interacts with Cdc31p (centrin), which is a component of the spindle pole body; part of the RAM network that regulates cellular polarity and morphogenesis [Source:SGD;Acc:S000001144]) HSP 1 Score: 159.458 bits (402), Expect = 1.810e-40 Identity = 86/212 (40.57%), Postives = 126/212 (59.43%), Query Frame = 2
Query: 1043 DPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKK-DLILNEILVIKANKH-QNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRK-QYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRAL 1669
D S ++ + IG+G GVV G +++T + AIK +NL + + + EI + + K NI Y S++ + LW++ME+ GGSL ++ +DE I I RE+ +AL +H ++VIHRDIK+ N+L+ +G+VK+ DFG AQ+ R TM GTPYWMAPEV+ Y KVD+WSLGI E+ G PPY + LRA+
Sbjct: 18 DVSSLFKRTEVIGRGKFGVVYKGYNVKTGRVYAIKVLNLDSDSDEVEDVQREIQFLASLKQISNITRYYGSYLKDTSLWIIMEHCAGGSLRSLLRPGKIDEKYIGVIMRELLVALKCIHKDNVIHRDIKAANVLITNEGNVKLCDFGVAAQVNQTSLRRQTMAGTPYWMAPEVIMEGVYYDTKVDIWSLGITTYEIATGNPPYCDVEALRAM 229
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Yeast
Match: SPS1 (Putative protein serine/threonine kinase; localizes to the nucleus and cytoplasm; required for efficient spore packaging, prospore membrane development and closure and localization of enzymes involved in spore wall synthesis; interacts with and required for Ssp1p phosphorylation and turnover; member of the GCKIII subfamily of STE20 kinases; multiply phosphorylated on S/T residues; interacts with 14-3-3 proteins, Bmh1p and Bmh2p; expressed at the end of meiosis [Source:SGD;Acc:S000002931]) HSP 1 Score: 151.754 bits (382), Expect = 1.347e-39 Identity = 93/239 (38.91%), Postives = 134/239 (56.07%), Query Frame = 2
Query: 1013 SKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKK-DLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMD---EFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRK--QYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALFNSNKWK-AFIEGP 1708
SK + S + P Y I IG+G G V D T++ VAIK +NL+ + +L+ EI + K I NY+ + + + +W+VMEY GGS +D++ + ++ E +++ I EVT+ L +LH IHRDIK+ NILL +G VK+ DFG I R T VGTPYWMAPEVV + Y K D+WSLGI E+L+G PP +P++ + N K K ++GP
Sbjct: 3 SKEISIRSRTPPSKLYSIQSCIGRGNFGDVYKAVDRVTQEIVAIKVVNLEHSDEDIELLAQEIFFLAELKSPLITNYIATMLEDVSMWIVMEYCGGGSCSDLLKRSYVNGLPEEKVSFIIHEVTLGLKYLHEQRKIHRDIKAANILLNEEGMVKLGDFGVSGH-IRSTLKRDTFVGTPYWMAPEVVCCEVDGYNEKADIWSLGITTYELLKGLPPLSKYDPMKVMTNLPKRKPPKLQGP 240
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Nematostella
Match: EDO33667 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRJ0]) HSP 1 Score: 344.354 bits (882), Expect = 6.837e-112 Identity = 161/240 (67.08%), Postives = 191/240 (79.58%), Query Frame = 2
Query: 962 HQQLRRADRH---CSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
H Q++ D+ S E +++L+ +VS DP KY ++IGQGASG V ++ T +VAIKQMNL QQPKK+LI+NEILV++ NKH NIVNY+DS+++ ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DG VK+ DFGFCA + +N RSTMVGTPYWMAPEVVTRKQYGP+VD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 191 HGQVQARDKRKKKMSDEEILARLRTIVSIGDPKKKYTKFEKIGQGASGTVYTAIEVATGHEVAIKQMNLSQQPKKELIINEILVMRENKHPNIVNYVDSYLVGEELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNGVIHRDIKSDNILLGMDGQVKLTDFGFCATITPEQNKRSTMVGTPYWMAPEVVTRKQYGPRVDVWSLGIMAIEMVEGEPPYLNENPLRALY 430 HSP 2 Score: 79.7221 bits (195), Expect = 6.837e-112 Identity = 39/71 (54.93%), Postives = 54/71 (76.06%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
L ALYLIATNG P L P +SP+F DFL + LE+D++KR+SA LL+H F+ ++KP++ L+PLIL A+E
Sbjct: 426 LRALYLIATNGTPELAHPEKLSPVFKDFLAQSLEMDVEKRSSARELLQHPFL-KQAKPLASLVPLILAAKE 495 HSP 3 Score: 67.0106 bits (162), Expect = 1.834e-11 Identity = 46/110 (41.82%), Postives = 57/110 (51.82%), Query Frame = 2
Query: 71 DSELPPAPPIRSSSTLRRKDRYSVSXXXXXXXXXXXXXXXXXXXXXXXF-------NYNDPKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
D E PPAPPIR +S KD S S P ++KK K F N P+IS PT H HV FDP TG+F G+P +W L+ SNI++ EQK NP+
Sbjct: 3 DEERPPAPPIRLTS----KDA-SNSLGSKPLPNAPVEEKKKKTATFRLFPNKTEYNKKNKPEISYPTQFEHTIHVGFDPVTGEFTGMPEAWARLLQVSNITQAEQKKNPQ 107
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Nematostella
Match: EDO45846 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRL1]) HSP 1 Score: 246.128 bits (627), Expect = 8.988e-75 Identity = 115/230 (50.00%), Postives = 165/230 (71.74%), Query Frame = 2
Query: 980 ADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRAL 1669
+D + E F L+ VV S+ +IG+G++G+V D T ++VA+K+M+LK+Q +++L+ NE+++++ H NIV S ++ +ELWVVME+L+GG+LTD+VT + E Q+A +CR V AL FLHS VIHRDIKSD+ILL +G+VK+ DFGFCAQ+ R ++VGTPYWMAPEV++RK YG +VD+WSLGIMV+EM++GEPPY NE PL+A+
Sbjct: 3 SDTPVTHEQFRRHLEVVVQSTRQPENLGNFMKIGEGSTGIVCLATDKRTGRQVAVKKMDLKKQQRRELLFNEVVIMRDYPHPNIVEMYGSHLVGDELWVVMEFLEGGTLTDIVTHTNLSEEQVACVCRAVLKALTFLHSQGVIHRDIKSDSILLTSNGTVKLSDFGFCAQVTEDMPRRKSLVGTPYWMAPEVISRKPYGTEVDIWSLGIMVLEMVDGEPPYFNEPPLQAM 232
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Nematostella
Match: EDO31934 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SWI4]) HSP 1 Score: 179.874 bits (455), Expect = 1.074e-49 Identity = 90/212 (42.45%), Postives = 130/212 (61.32%), Query Frame = 2
Query: 1040 SDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILN-EILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+DP + L+RIG+G+ G V G D T + VAIK ++L++ + + EI V+ + Y S++ +LW++MEYL GGS D++ ++EF IATI REV L +LH+ +HRDIK+ N+L+ G VK+ DFG QL N R+T VGTP+WMAPEV+ + Y K D+WSLGI IE+ +GEPP + +P+R LF
Sbjct: 5 ADPEKLFSKLERIGKGSFGEVFKGIDNRTNEVVAIKIIDLEEAEDEIEDIQQEITVLSQCDSLYVTKYHGSYLKGTKLWIIMEYLGGGSALDLMKAGNIEEFYIATILREVLKGLDYLHTEKKLHRDIKAANVLMSETGDVKLADFGVAGQLTDTLNKRNTFVGTPFWMAPEVIKQSAYDSKADIWSLGITAIELAKGEPPNSDLHPMRVLF 216
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Nematostella
Match: EDO42845 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0A9]) HSP 1 Score: 160.229 bits (404), Expect = 6.397e-48 Identity = 79/211 (37.44%), Postives = 125/211 (59.24%), Query Frame = 2
Query: 1046 PYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDV--VTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
P +++L+++G+G+ G V E+ + VAIKQ+ + ++ I+ EI +++ +V Y S+ N +LW+VMEY GS++D+ + + E +I + + L +LH IHRDIK+ NILL +G K+ DFG QL R+T++GTP+WMAPEV+ Y D+WSLGI +EM EG+PPY + +P+RA+F
Sbjct: 20 PEEVFDVLEKLGEGSYGSVFKAMHKESGQVVAIKQVPVDTDLQE--IIKEISMMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDLMKIRNKTLTEEEIKCVLKYTLKGLEYLHLRRKIHRDIKAGNILLNSEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEVGYDCLADIWSLGITAMEMAEGKPPYADIHPMRAIF 228 HSP 2 Score: 50.0618 bits (118), Expect = 6.397e-48 Identity = 26/67 (38.81%), Postives = 42/67 (62.69%), Query Frame = 3
Query: 1662 ALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLA 1862
A+++I T P+ +DP S F DF+ +CL + ++RA+A+ LL+H FI +K +SIL +I A
Sbjct: 226 AIFMIPTKPPPTFRDPGQWSEDFKDFVSKCLVKNPEERATATELLQHSFIQG-AKQISILSSMIQEA 291
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Nematostella
Match: EDO40961 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S5T6]) HSP 1 Score: 169.474 bits (428), Expect = 7.864e-47 Identity = 90/229 (39.30%), Postives = 135/229 (58.95%), Query Frame = 2
Query: 1028 VVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFI------MNNELWVVMEYLDGGSLTDVVTEA---CMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQ-----YGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
+ + DP +++++ +G G G V GR T + AIK M++ + ++++ L ++ K + H+NI Y +FI +++LW+VME+ GS+TD+V A + E I+ ICREV + LH NHVIHRDIK N+LL VK++DFG AQL R+T +GTPYWMAPEV+ + Y + D+WS+GI IEM E +PP + +P+RALF
Sbjct: 10 LAALKDPAGIFDLVEVVGNGTYGQVHKGRHKRTGQLTAIKIMDVTEDEEEEIKLEINVLRKFSNHRNIATYYGAFIKKSLQGQDDQLWLVMEFCGAGSVTDLVKTARNKALKEEWISYICREVLNGVSHLHMNHVIHRDIKGQNVLLTESAEVKLVDFGVSAQLDRTIGRRNTFIGTPYWMAPEVIACDEQPDATYDNRCDMWSIGITAIEMAESQPPLCDMHPMRALF 238
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Medaka
Match: PAK3 (serine/threonine-protein kinase PAK 3 [Source:NCBI gene;Acc:101162700]) HSP 1 Score: 356.295 bits (913), Expect = 4.447e-116 Identity = 165/249 (66.27%), Postives = 196/249 (78.71%), Query Frame = 2
Query: 926 IEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
++ + N RH +R + E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ +N RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 235 VQPENTSNTMYRHTDRQRKKSKMTDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAIDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNSNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQNKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 483 HSP 2 Score: 83.5741 bits (205), Expect = 4.447e-116 Identity = 41/71 (57.75%), Postives = 54/71 (76.06%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
L ALYLIATNG P L++P +S +F DFL+RCLE+D+D+R SA LL+H F+ +KP+S L PLIL A+E
Sbjct: 479 LRALYLIATNGTPELQNPERLSSVFRDFLNRCLEMDVDRRGSAKDLLQHSFLK-LAKPLSSLTPLILAAKE 548 HSP 3 Score: 58.151 bits (139), Expect = 2.841e-8 Identity = 25/50 (50.00%), Postives = 32/50 (64.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K EQK NP+
Sbjct: 67 PEISLPSDFEHTIHVGFDAVTGEFTGIPEQWARLLQTSNITKLEQKKNPQ 116
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Medaka
Match: pak1 (p21 (RAC1) activated kinase 1 [Source:NCBI gene;Acc:101164663]) HSP 1 Score: 347.436 bits (890), Expect = 7.768e-114 Identity = 162/237 (68.35%), Postives = 191/237 (80.59%), Query Frame = 2
Query: 962 HQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
H Q + + S E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK Y PKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 256 HSQDKTRKKKMSDEEILEKLRTIVSVGDPKKKYTRFEKIGQGASGTVYTAIDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYCPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 492 HSP 2 Score: 85.1149 bits (209), Expect = 7.768e-114 Identity = 41/74 (55.41%), Postives = 57/74 (77.03%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSE 1877
L ALYLIATNG P L++P +S +F DFL+RCLE+D++KR SA LL+H+F+ +KP+S L PLIL A+E ++
Sbjct: 488 LRALYLIATNGTPELQNPEKLSTIFRDFLNRCLEMDVEKRGSAKELLQHQFLK-MAKPLSSLTPLILAAKEAAK 560 HSP 3 Score: 58.5362 bits (140), Expect = 2.142e-8 Identity = 25/50 (50.00%), Postives = 32/50 (64.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K EQK NP+
Sbjct: 73 PEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKLEQKKNPQ 122
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Medaka
Match: PAK3 (serine/threonine-protein kinase PAK 3 [Source:NCBI gene;Acc:101162700]) HSP 1 Score: 356.295 bits (913), Expect = 2.578e-112 Identity = 165/249 (66.27%), Postives = 196/249 (78.71%), Query Frame = 2
Query: 926 IEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
++ + N RH +R + E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ +N RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 256 VQPENTSNTMYRHTDRQRKKSKMTDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAIDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNSNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQNKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 504 HSP 2 Score: 83.5741 bits (205), Expect = 3.674e-16 Identity = 41/77 (53.25%), Postives = 57/77 (74.03%), Query Frame = 3
Query: 1638 HISMKILCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
+++ L ALYLIATNG P L++P +S +F DFL+RCLE+D+D+R SA LL+H F+ +KP+S L PLIL A+E
Sbjct: 494 YLNENPLRALYLIATNGTPELQNPERLSSVFRDFLNRCLEMDVDRRGSAKDLLQHSFLK-LAKPLSSLTPLILAAKE 569 HSP 3 Score: 58.151 bits (139), Expect = 2.865e-8 Identity = 25/50 (50.00%), Postives = 32/50 (64.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K EQK NP+
Sbjct: 67 PEISLPSDFEHTIHVGFDAVTGEFTGIPEQWARLLQTSNITKLEQKKNPQ 116
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Medaka
Match: PAK3 (serine/threonine-protein kinase PAK 3 [Source:NCBI gene;Acc:101162700]) HSP 1 Score: 356.295 bits (913), Expect = 2.578e-112 Identity = 165/249 (66.27%), Postives = 196/249 (78.71%), Query Frame = 2
Query: 926 IEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
++ + N RH +R + E + KL+ +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ +N RSTMVGTPYWMAPEVVTRK YGPKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 256 VQPENTSNTMYRHTDRQRKKSKMTDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAIDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNSNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALDFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQNKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 504 HSP 2 Score: 83.5741 bits (205), Expect = 3.674e-16 Identity = 41/77 (53.25%), Postives = 57/77 (74.03%), Query Frame = 3
Query: 1638 HISMKILCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLARE 1868
+++ L ALYLIATNG P L++P +S +F DFL+RCLE+D+D+R SA LL+H F+ +KP+S L PLIL A+E
Sbjct: 494 YLNENPLRALYLIATNGTPELQNPERLSSVFRDFLNRCLEMDVDRRGSAKDLLQHSFLK-LAKPLSSLTPLILAAKE 569 HSP 3 Score: 58.151 bits (139), Expect = 2.865e-8 Identity = 25/50 (50.00%), Postives = 32/50 (64.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K EQK NP+
Sbjct: 67 PEISLPSDFEHTIHVGFDAVTGEFTGIPEQWARLLQTSNITKLEQKKNPQ 116
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Medaka
Match: pak1 (p21 (RAC1) activated kinase 1 [Source:NCBI gene;Acc:101164663]) HSP 1 Score: 342.043 bits (876), Expect = 2.637e-112 Identity = 163/245 (66.53%), Postives = 191/245 (77.96%), Query Frame = 2
Query: 962 HQQLRRADRHCSGEFFISKLKG--------VVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
H Q + + S E + KL G +VS DP KY ++IGQGASG V D+ T ++VAIKQMNL+QQPKK+LI+NEILV++ NK+ NIVNYLDS+++ +ELWVVMEYL GGSLTDVVTE CMDE QIA +CRE AL FLHSN VIHRDIKSDNILLG+DGSVK+ DFGFCAQ+ ++ RSTMVGTPYWMAPEVVTRK Y PKVD+WSLGIM IEM+EGEPPYLNENPLRAL+
Sbjct: 256 HSQDKTRKKKMSDEEILEKLHGLRSPSAGTIVSVGDPKKKYTRFEKIGQGASGTVYTAIDIATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYCPKVDIWSLGIMAIEMVEGEPPYLNENPLRALY 500 HSP 2 Score: 85.1149 bits (209), Expect = 2.637e-112 Identity = 41/74 (55.41%), Postives = 57/74 (77.03%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSE 1877
L ALYLIATNG P L++P +S +F DFL+RCLE+D++KR SA LL+H+F+ +KP+S L PLIL A+E ++
Sbjct: 496 LRALYLIATNGTPELQNPEKLSTIFRDFLNRCLEMDVEKRGSAKELLQHQFLK-MAKPLSSLTPLILAAKEAAK 568 HSP 3 Score: 58.5362 bits (140), Expect = 2.054e-8 Identity = 25/50 (50.00%), Postives = 32/50 (64.00%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPE 379
P+IS P+ H HV FD TG+F G+P W L+ SNI+K EQK NP+
Sbjct: 73 PEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKLEQKKNPQ 122
BLAST of Non-specific serine/threonine protein kinase vs. Planmine SMEST
Match: SMESG000009652.1 (SMESG000009652.1) HSP 1 Score: 956.822 bits (2472), Expect = 0.000e+0 Identity = 551/572 (96.33%), Postives = 555/572 (97.03%), Query Frame = 2
Query: 29 MDNKSNSIEINETLDSELPPAPPIRSSSTLRRKDRYSVSXXXXXXXXXXXXXXXXXXXXXXXFNYNDPKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPEXXXXXXXXXXXXXXXEPKYFQDREIFYQPPIVPPHLPINEKDLEIKPQLVNNVTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQCQYSEKVSTLTATSSIHPSQMPNSKRESSGIVADASSFECISCQKEGNNKPENIITDQFTELKSPLLEVETDELGETGDSVTPCDKSKDSVSIEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF-NSNKWKAFIEGPNNSFAFILRFF 1741
MDNKSNSIEINETLDSELPPAPPIRSSSTLRRKDRYSVSKPLPSPPIKKEKKKKTKLFKLPKFNYNDPKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPELLLDVLQCLDLSQQSEPKYFQDREIFYQPPIVPPHLPINEKDLEIKPQLVNNVTSGSPSDRPASSSGSHSSRDSSNSSRSSSSNSPDCSQCQYSEKVSTLTATSSIHPSQMPNSKRESSGIVADASSFECISCQKEGNNKPENIITDQFTELKSPLLEVETDELGETGDSVTPCDKSKDSVSIEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRAL+ + K ++ P L F
Sbjct: 1 MDNKSNSIEINETLDSELPPAPPIRSSSTLRRKDRYSVSKPLPSPPIKKEKKKKTKLFKLPKFNYNDPKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPELLLDVLQCLDLSQQSEPKYFQDREIFYQPPIVPPHLPINEKDLEIKPQLVNNVTSGSPSDRPASSSGSHSSRDSSNSSRSSSSNSPDCSQCQYSEKVSTLTATSSIHPSQMPNSKRESSGIVADASSFECISCQKEGNNKPENIITDQFTELKSPLLEVETDELGETGDSVTPCDKSKDSVSIEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALYLIATNGKPSLKDPTTVSPLFLDFL 572 HSP 2 Score: 154.451 bits (389), Expect = 6.041e-39 Identity = 90/145 (62.07%), Postives = 104/145 (71.72%), Query Frame = 3
Query: 1500 VEIIDQQWLEHLIGWHQKLL--------LENNMVLKWICGP-LELW-----LLKCWKGSL-HISMKILCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSEICEQ 1889
V+IID + LIG + +V + GP ++LW +++ +G +++ L ALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSEICEQ
Sbjct: 477 VKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSEICEQ 621
BLAST of Non-specific serine/threonine protein kinase vs. Planmine SMEST
Match: SMESG000009652.1 (SMESG000009652.1) HSP 1 Score: 610.527 bits (1573), Expect = 0.000e+0 Identity = 292/292 (100.00%), Postives = 292/292 (100.00%), Query Frame = 2
Query: 878 TGDSVTPCDKSKDSVSIEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALFNSNKWKAFIEGPNNSFAFILRFFGPLP 1753
TGDSVTPCDKSKDSVSIEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALFNSNKWKAFIEGPNNSFAFILRFFGPLP
Sbjct: 4 TGDSVTPCDKSKDSVSIEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALFNSNKWKAFIEGPNNSFAFILRFFGPLP 295
BLAST of Non-specific serine/threonine protein kinase vs. Planmine SMEST
Match: SMESG000009652.1 (SMESG000009652.1) HSP 1 Score: 554.288 bits (1427), Expect = 0.000e+0 Identity = 264/265 (99.62%), Postives = 265/265 (100.00%), Query Frame = 2
Query: 878 TGDSVTPCDKSKDSVSIEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
TGDSVTPCDKSKDSVSIEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRAL+
Sbjct: 4 TGDSVTPCDKSKDSVSIEINQSQNRCDRHQQLRRADRHCSGEFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMNNELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALY 268 HSP 2 Score: 153.68 bits (387), Expect = 0.000e+0 Identity = 77/78 (98.72%), Postives = 77/78 (98.72%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSEICEQ 1889
L ALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSEICEQ
Sbjct: 264 LRALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQSEICEQ 341
BLAST of Non-specific serine/threonine protein kinase vs. Planmine SMEST
Match: SMESG000009652.1 (SMESG000009652.1) HSP 1 Score: 407.912 bits (1047), Expect = 4.235e-136 Identity = 285/285 (100.00%), Postives = 285/285 (100.00%), Query Frame = 2
Query: 29 MDNKSNSIEINETLDSELPPAPPIRSSSTLRRKDRYSVSXXXXXXXXXXXXXXXXXXXXXXXFNYNDPKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPEXXXXXXXXXXXXXXXEPKYFQDREIFYQPPIVPPHLPINEKDLEIKPQLVNNVTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQCQYSEKVSTLTATSSIHPSQMPNSKRESSGIVADASSFECISCQKEGNNKPENIITDQFTELKSPLLEVETDELGETG 883
MDNKSNSIEINETLDSELPPAPPIRSSSTLRRKDRYSVSKPLPSPPIKKEKKKKTKLFKLPKFNYNDPKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPELLLDVLQCLDLSQQSEPKYFQDREIFYQPPIVPPHLPINEKDLEIKPQLVNNVTSGSPSDRPASSSGSHSSRDSSNSSRSSSSNSPDCSQCQYSEKVSTLTATSSIHPSQMPNSKRESSGIVADASSFECISCQKEGNNKPENIITDQFTELKSPLLEVETDELGETG
Sbjct: 1 MDNKSNSIEINETLDSELPPAPPIRSSSTLRRKDRYSVSKPLPSPPIKKEKKKKTKLFKLPKFNYNDPKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPELLLDVLQCLDLSQQSEPKYFQDREIFYQPPIVPPHLPINEKDLEIKPQLVNNVTSGSPSDRPASSSGSHSSRDSSNSSRSSSSNSPDCSQCQYSEKVSTLTATSSIHPSQMPNSKRESSGIVADASSFECISCQKEGNNKPENIITDQFTELKSPLLEVETDELGETG 285
BLAST of Non-specific serine/threonine protein kinase vs. Planmine SMEST
Match: SMESG000008326.1 (SMESG000008326.1) HSP 1 Score: 340.117 bits (871), Expect = 2.656e-114 Identity = 161/230 (70.00%), Postives = 187/230 (81.30%), Query Frame = 2
Query: 1001 EFFISKLKGVVSSSDPYSKYEILDRIGQGASGVVMGGRDLETKKKVAIKQMNLKQQPKKDLILNEILVIKANKHQNIVNYLDSFIMN------NELWVVMEYLDGGSLTDVVTEACMDEFQIATICREVTMALGFLHSNHVIHRDIKSDNILLGLDGSVKIIDFGFCAQLIGGRNNRSTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMVIEMLEGEPPYLNENPLRALF 1672
E KL+ +VS DP KY+ ++IGQGASGVV G + T KKVAIKQM LKQQPKK+LI+NEI V+KAN+H N+VNYLDS+++ +ELWVVMEYLDGGSLT+VVTE MDE IA +CRE AL FLH+N+VIHRDIKSDNILLGLDGSVK+ DFGFCAQL + RSTMVGTPYWMAPEVVTRKQYGPKVD+WSLGIM +EM++GEPPYLNENPLRAL+
Sbjct: 331 EEICEKLRKIVSLGDPNRKYKKHEKIGQGASGVVYNGIETSTGKKVAIKQMQLKQQPKKELIINEICVMKANRHPNVVNYLDSYLVGGTGTVADELWVVMEYLDGGSLTEVVTETFMDEGHIAAVCRECLQALDFLHTNNVIHRDIKSDNILLGLDGSVKLTDFGFCAQLNNEQTKRSTMVGTPYWMAPEVVTRKQYGPKVDVWSLGIMALEMIDGEPPYLNENPLRALY 560 HSP 2 Score: 93.2041 bits (230), Expect = 2.656e-114 Identity = 45/73 (61.64%), Postives = 59/73 (80.82%), Query Frame = 3
Query: 1656 LCALYLIATNGKPSLKDPTTVSPLFLDFLDRCLEVDIDKRASASSLLKHEFITDKSKPVSILIPLILLAREQS 1874
L ALYLIATNGKP +K+ + +S +FLDFLD+CLEV ++KR SAS LL H FI K+KP+S L PLI++A+EQ+
Sbjct: 556 LRALYLIATNGKPEIKEKSRLSVVFLDFLDKCLEVQVEKRISASELLTHPFIVRKAKPLSSLTPLIVVAKEQA 628 HSP 3 Score: 51.9878 bits (123), Expect = 1.775e-6 Identity = 29/72 (40.28%), Postives = 43/72 (59.72%), Query Frame = 2
Query: 230 PKISSPTYVSHNFHVVFDPTTGDFQGLPVSWKEWLKASNISKTEQKDNPEXXXXXXXXXXXXXXXEPKYFQD 445
P IS+P V H H+ F TG+F G+P+ W+ L+ +NISK EQ+ NP +L+ L L +++ KY D
Sbjct: 69 PNISAPISVMHKVHITFSQDTGEFAGVPLEWQVILE-NNISKIEQQQNPRAVLNALNILVDKNKNKEKYMMD 139 The following BLAST results are available for this feature:
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Non-specific serine/threonine protein kinase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30027002 ID=SMED30027002|Name=Non-specific serine/threonine protein kinase|organism=Schmidtea mediterranea sexual|type=transcript|length=2600bpback to top protein sequence of SMED30027002-orf-1 >SMED30027002-orf-1 ID=SMED30027002-orf-1|Name=SMED30027002-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=107bp MVLKWICGPLELWLLKCWKGSLHISMKILCALYLIATNGKPSLKDPTTVSback to top protein sequence of SMED30027002-orf-2 >SMED30027002-orf-2 ID=SMED30027002-orf-2|Name=SMED30027002-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=576bp MDNKSNSIEINETLDSELPPAPPIRSSSTLRRKDRYSVSKPLPSPPIKKEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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