Eukaryotic translation initiation factor 3 subunit C

Overview
NameEukaryotic translation initiation factor 3 subunit C
Smed IDSMED30026999
Length (bp)732
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Eukaryotic translation initiation factor 3 subunit C (SMED30026999) t-SNE clustered cells

Violin plots show distribution of expression levels for Eukaryotic translation initiation factor 3 subunit C (SMED30026999) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Eukaryotic translation initiation factor 3 subunit C (SMED30026999) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Eukaryotic translation initiation factor 3 subunit C (SMED30026999) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30026999

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
posterior sideSMED30026999h1SMcG0017128 dd_Smed_v4_5221_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30026999h1SMcG0017128 dd_Smed_v4_5221_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Eukaryotic translation initiation factor 3 subunit C vs. Ensembl Fly
Match: CG31688 (gene:FBgn0263355 transcript:FBtr0308895)

HSP 1 Score: 178.333 bits (451), Expect = 4.193e-56
Identity = 76/137 (55.47%), Postives = 105/137 (76.64%), Query Frame = 3 
Query:   93 WFQKEIILDKYSRGCHYITDVVIKTLPEIKNYKIGILHLFMKHTSASLTLNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHSVSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQG 503
            WFQ++I L    RG H +T+ +++ +PE+ ++ +G+ H+ + HTSASL LNE++D DV+ DMEMMLNKIVPE L Y+HSCEGPDDMPAHVK   LG  S+++PI+DG L+LGTWQG+W CEHR+    RK++ T+ G
Sbjct:   37 WFQRKINLRPQHRGVHLVTEEILRQMPELAHFSVGLCHMQILHTSASLALNESWDPDVRDDMEMMLNKIVPEGLPYRHSCEGPDDMPAHVKACFLGS-SLTIPITDGKLSLGTWQGVWLCEHRDQAGSRKLVITLTG 172
[Close]
BLAST of Eukaryotic translation initiation factor 3 subunit C vs. Ensembl Fly
Match: CG31688 (gene:FBgn0263355 transcript:FBtr0273378)

HSP 1 Score: 178.333 bits (451), Expect = 4.193e-56
Identity = 76/137 (55.47%), Postives = 105/137 (76.64%), Query Frame = 3 
Query:   93 WFQKEIILDKYSRGCHYITDVVIKTLPEIKNYKIGILHLFMKHTSASLTLNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHSVSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQG 503
            WFQ++I L    RG H +T+ +++ +PE+ ++ +G+ H+ + HTSASL LNE++D DV+ DMEMMLNKIVPE L Y+HSCEGPDDMPAHVK   LG  S+++PI+DG L+LGTWQG+W CEHR+    RK++ T+ G
Sbjct:   37 WFQRKINLRPQHRGVHLVTEEILRQMPELAHFSVGLCHMQILHTSASLALNESWDPDVRDDMEMMLNKIVPEGLPYRHSCEGPDDMPAHVKACFLGS-SLTIPITDGKLSLGTWQGVWLCEHRDQAGSRKLVITLTG 172
[Close]
BLAST of Eukaryotic translation initiation factor 3 subunit C vs. Ensembl Fly
Match: CG31688 (gene:FBgn0263355 transcript:FBtr0330661)

HSP 1 Score: 178.333 bits (451), Expect = 4.193e-56
Identity = 76/137 (55.47%), Postives = 105/137 (76.64%), Query Frame = 3 
Query:   93 WFQKEIILDKYSRGCHYITDVVIKTLPEIKNYKIGILHLFMKHTSASLTLNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHSVSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQG 503
            WFQ++I L    RG H +T+ +++ +PE+ ++ +G+ H+ + HTSASL LNE++D DV+ DMEMMLNKIVPE L Y+HSCEGPDDMPAHVK   LG  S+++PI+DG L+LGTWQG+W CEHR+    RK++ T+ G
Sbjct:   37 WFQRKINLRPQHRGVHLVTEEILRQMPELAHFSVGLCHMQILHTSASLALNESWDPDVRDDMEMMLNKIVPEGLPYRHSCEGPDDMPAHVKACFLGS-SLTIPITDGKLSLGTWQGVWLCEHRDQAGSRKLVITLTG 172
[Close]
BLAST of Eukaryotic translation initiation factor 3 subunit C vs. Ensembl Fly
Match: CG31688 (gene:FBgn0263355 transcript:FBtr0330203)

HSP 1 Score: 178.718 bits (452), Expect = 4.210e-56
Identity = 76/137 (55.47%), Postives = 105/137 (76.64%), Query Frame = 3 
Query:   93 WFQKEIILDKYSRGCHYITDVVIKTLPEIKNYKIGILHLFMKHTSASLTLNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHSVSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQG 503
            WFQ++I L    RG H +T+ +++ +PE+ ++ +G+ H+ + HTSASL LNE++D DV+ DMEMMLNKIVPE L Y+HSCEGPDDMPAHVK   LG  S+++PI+DG L+LGTWQG+W CEHR+    RK++ T+ G
Sbjct:   37 WFQRKINLRPQHRGVHLVTEEILRQMPELAHFSVGLCHMQILHTSASLALNESWDPDVRDDMEMMLNKIVPEGLPYRHSCEGPDDMPAHVKACFLGS-SLTIPITDGKLSLGTWQGVWLCEHRDQAGSRKLVITLTG 172
[Close]
BLAST of Eukaryotic translation initiation factor 3 subunit C vs. Ensembl Fly
Match: CG31688 (gene:FBgn0263355 transcript:FBtr0330204)

HSP 1 Score: 181.03 bits (458), Expect = 2.169e-52
Identity = 76/137 (55.47%), Postives = 105/137 (76.64%), Query Frame = 3 
Query:   93 WFQKEIILDKYSRGCHYITDVVIKTLPEIKNYKIGILHLFMKHTSASLTLNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHSVSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQG 503
            WFQ++I L    RG H +T+ +++ +PE+ ++ +G+ H+ + HTSASL LNE++D DV+ DMEMMLNKIVPE L Y+HSCEGPDDMPAHVK   LG  S+++PI+DG L+LGTWQG+W CEHR+    RK++ T+ G
Sbjct:   37 WFQRKINLRPQHRGVHLVTEEILRQMPELAHFSVGLCHMQILHTSASLALNESWDPDVRDDMEMMLNKIVPEGLPYRHSCEGPDDMPAHVKACFLGS-SLTIPITDGKLSLGTWQGVWLCEHRDQAGSRKLVITLTG 172
[Close]
BLAST of Eukaryotic translation initiation factor 3 subunit C vs. UniProt/SwissProt
Match: sp|P0AF48|YJBQ_ECOLI (UPF0047 protein YjbQ OS=Escherichia coli (strain K12) OX=83333 GN=yjbQ PE=3 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 5.428e-46
Identity = 71/138 (51.45%), Postives = 94/138 (68.12%), Query Frame = 3 
Query:   90 MWFQKEIILDKYSRGCHYITDVVIKTLPEIKNYKIGILHLFMKHTSASLTLNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHSVSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQG 503
            MW+QK + L   SRG H +TD ++  L ++    IG+LHL ++HTSASLTLNEN D  V+ DME    + VP+N NY+H  EG DDMP+H+K S+LG  S+ +P+  G +  GTWQGIW  EHR H   R+IIAT+QG
Sbjct:    1 MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQHTSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGT-SLVLPVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQG 137
[Close]
BLAST of Eukaryotic translation initiation factor 3 subunit C vs. UniProt/SwissProt
Match: sp|P0AF49|YJBQ_ECO57 (UPF0047 protein YjbQ OS=Escherichia coli O157:H7 OX=83334 GN=yjbQ PE=3 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 5.428e-46
Identity = 71/138 (51.45%), Postives = 94/138 (68.12%), Query Frame = 3 
Query:   90 MWFQKEIILDKYSRGCHYITDVVIKTLPEIKNYKIGILHLFMKHTSASLTLNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHSVSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQG 503
            MW+QK + L   SRG H +TD ++  L ++    IG+LHL ++HTSASLTLNEN D  V+ DME    + VP+N NY+H  EG DDMP+H+K S+LG  S+ +P+  G +  GTWQGIW  EHR H   R+IIAT+QG
Sbjct:    1 MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQHTSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGT-SLVLPVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQG 137
[Close]
BLAST of Eukaryotic translation initiation factor 3 subunit C vs. UniProt/SwissProt
Match: sp|O14155|YE72_SCHPO (UPF0047 protein C4A8.02c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC4A8.02c PE=3 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 3.503e-43
Identity = 67/136 (49.26%), Postives = 99/136 (72.79%), Query Frame = 3 
Query:   96 FQKEIILDKYSRGCHYITDVVIKTLPEIKNYKIGILHLFMKHTSASLTLNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHSVSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQG 503
             Q+ I LD+ S+G + IT+ ++K LPE+K++  G ++ F++HTSA+LT+NEN+D+D + DM  +L+KIVPE+  Y+H+ EG DDMPAHVK SL+ G S+++PI++G L+LGTWQ I   E R     R I+ TI G
Sbjct:    4 IQRIITLDRRSKGFYIITNDLVKKLPELKSFSSGTVNFFIQHTSAALTINENWDADTRADMNDILDKIVPESAGYRHTAEGLDDMPAHVKSSLI-GPSLTVPITNGKLSLGTWQDIQLAEFRRQPHSRTIVCTIIG 138
[Close]
BLAST of Eukaryotic translation initiation factor 3 subunit C vs. UniProt/SwissProt
Match: sp|P0A2L1|YJBQ_SALTY (UPF0047 protein YjbQ OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=yjbQ PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 9.086e-40
Identity = 73/138 (52.90%), Postives = 91/138 (65.94%), Query Frame = 3 
Query:   90 MWFQKEIILDKYSRGCHYITDVVIKTLPEIKNYKIGILHLFMKHTSASLTLNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHSVSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQG 503
            MW+Q+ I L +  RG H ITD +   L  +   + G+LHL + HTSASLTLNEN D  V+ DME    K VP+N  Y+H  EG DDMP+H+K S+L G S+ +P+  G L LGTWQGIW  EHR H   RKIIAT+QG
Sbjct:    1 MWYQRTITLSEKPRGFHLITDEITDKLSGLPPVETGLLHLLLLHTSASLTLNENCDPTVRADMERHFLKTVPDNAAYEHDYEGADDMPSHIKSSVL-GVSLLLPVRQGRLQLGTWQGIWLGEHRIHGGPRKIIATLQG 137
[Close]
BLAST of Eukaryotic translation initiation factor 3 subunit C vs. UniProt/SwissProt
Match: sp|P0A2L2|YJBQ_SALTI (UPF0047 protein YjbQ OS=Salmonella typhi OX=90370 GN=yjbQ PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 9.086e-40
Identity = 73/138 (52.90%), Postives = 91/138 (65.94%), Query Frame = 3 
Query:   90 MWFQKEIILDKYSRGCHYITDVVIKTLPEIKNYKIGILHLFMKHTSASLTLNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHSVSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQG 503
            MW+Q+ I L +  RG H ITD +   L  +   + G+LHL + HTSASLTLNEN D  V+ DME    K VP+N  Y+H  EG DDMP+H+K S+L G S+ +P+  G L LGTWQGIW  EHR H   RKIIAT+QG
Sbjct:    1 MWYQRTITLSEKPRGFHLITDEITDKLSGLPPVETGLLHLLLLHTSASLTLNENCDPTVRADMERHFLKTVPDNAAYEHDYEGADDMPSHIKSSVL-GVSLLLPVRQGRLQLGTWQGIWLGEHRIHGGPRKIIATLQG 137
[Close]
BLAST of Eukaryotic translation initiation factor 3 subunit C vs. TrEMBL
Match: A0A0X3Q0H3 (UPF0047 protein YjbQ (Fragment) OS=Schistocephalus solidus OX=70667 GN=YJBQ PE=4 SV=1)

HSP 1 Score: 211.846 bits (538), Expect = 2.876e-66
Identity = 93/138 (67.39%), Postives = 116/138 (84.06%), Query Frame = 3 
Query:   90 MWFQKEIILDKYSRGCHYITDVVIKTLPEIKNYKIGILHLFMKHTSASLTLNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHSVSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQG 503
            +WFQ+ I L    RGCHY+T+ VIK  PEI   KIG LHLFM+HTS+S+TLNE++D+ VK D+EM+LN++VPE LNYKHSCEGPDDMPAH KH++LGG +V++PI+DG +NLGTWQG+WF EHRNH S RKIIAT+QG
Sbjct:   20 VWFQRTIDLKPKPRGCHYVTEEVIKGFPEISQIKIGTLHLFMQHTSSSITLNESWDASVKDDVEMLLNRLVPEGLNYKHSCEGPDDMPAHGKHAILGGSNVTVPITDGRMNLGTWQGLWFIEHRNHASSRKIIATLQG 157
[Close]
BLAST of Eukaryotic translation initiation factor 3 subunit C vs. TrEMBL
Match: A0A564YXM4 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS10273 PE=4 SV=1)

HSP 1 Score: 208.764 bits (530), Expect = 3.737e-65
Identity = 89/138 (64.49%), Postives = 116/138 (84.06%), Query Frame = 3 
Query:   90 MWFQKEIILDKYSRGCHYITDVVIKTLPEIKNYKIGILHLFMKHTSASLTLNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHSVSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQG 503
            +W+Q+ I +   SRGCHYITD +IK +PEI   KIGIL+LFM+HTS S+TLNE++D DVK D+EM+LN+++PENL YKH+CEGPDDMPAH KH++L G +V++PI+DG +NLGTWQGIWF EHRN K+ RK+IAT+ G
Sbjct:    9 VWYQRTITISPRSRGCHYITDDIIKGMPEITQIKIGILNLFMQHTSCSITLNESWDGDVKEDIEMLLNRLIPENLKYKHACEGPDDMPAHAKHAILSGSNVTIPITDGKMNLGTWQGIWFIEHRNQKTTRKVIATLNG 146
[Close]
BLAST of Eukaryotic translation initiation factor 3 subunit C vs. TrEMBL
Match: A0A068YIQ6 (Expressed conserved protein OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000975400 PE=4 SV=1)

HSP 1 Score: 207.223 bits (526), Expect = 1.562e-64
Identity = 89/138 (64.49%), Postives = 115/138 (83.33%), Query Frame = 3 
Query:   90 MWFQKEIILDKYSRGCHYITDVVIKTLPEIKNYKIGILHLFMKHTSASLTLNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHSVSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQG 503
            +W+Q+ I +   SRGCHYITD +IK +PEI   KIG L+LFM+HTS S+TLNE++D DVK D+EM+LN+++PE LNYKHSCEGPDDMPAH KH++L G +V++PI+DG +NLGTWQG+WF EHRN KS RK+IAT+ G
Sbjct:    9 VWYQRTITIPPCSRGCHYITDDIIKGMPEITQIKIGTLNLFMQHTSCSITLNESWDGDVKDDIEMLLNRLIPEKLNYKHSCEGPDDMPAHAKHAILSGSNVTIPITDGKMNLGTWQGVWFIEHRNQKSSRKVIATLNG 146
[Close]
BLAST of Eukaryotic translation initiation factor 3 subunit C vs. TrEMBL
Match: A0A068WF16 (Expressed conserved protein OS=Echinococcus granulosus OX=6210 GN=EgrG_000975400 PE=4 SV=1)

HSP 1 Score: 207.223 bits (526), Expect = 1.562e-64
Identity = 89/138 (64.49%), Postives = 115/138 (83.33%), Query Frame = 3 
Query:   90 MWFQKEIILDKYSRGCHYITDVVIKTLPEIKNYKIGILHLFMKHTSASLTLNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHSVSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQG 503
            +W+Q+ I +   SRGCHYITD +IK +PEI   KIG L+LFM+HTS S+TLNE++D DVK D+EM+LN+++PE LNYKHSCEGPDDMPAH KH++L G +V++PI+DG +NLGTWQG+WF EHRN KS RK+IAT+ G
Sbjct:    9 VWYQRTITIPPCSRGCHYITDDIIKGMPEITQIKIGTLNLFMQHTSCSITLNESWDGDVKDDIEMLLNRLIPEKLNYKHSCEGPDDMPAHAKHAILSGSNVTIPITDGKMNLGTWQGVWFIEHRNQKSSRKVIATLNG 146
[Close]
BLAST of Eukaryotic translation initiation factor 3 subunit C vs. TrEMBL
Match: A0A5K3F609 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 204.912 bits (520), Expect = 1.151e-63
Identity = 90/138 (65.22%), Postives = 113/138 (81.88%), Query Frame = 3 
Query:   90 MWFQKEIILDKYSRGCHYITDVVIKTLPEIKNYKIGILHLFMKHTSASLTLNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHSVSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQG 503
            +WFQ+ I +    RGCHYITD +IK +PEI   KIG L+LFM+HTS SLTLNE++DSDVK D+EM+LN+++PE LNYKHSCEGPDDMP H KH++L G +V++PI+DG +NLGTWQGIWF EHRN KS RK+I T+ G
Sbjct:    9 VWFQRTITIPPKPRGCHYITDDIIKGMPEITQIKIGTLNLFMQHTSCSLTLNESWDSDVKDDIEMLLNRLIPEKLNYKHSCEGPDDMPGHGKHAILSGSNVTIPITDGKMNLGTWQGIWFIEHRNQKSTRKVIGTLTG 146
[Close]
BLAST of Eukaryotic translation initiation factor 3 subunit C vs. Ensembl Nematostella
Match: EDO31717 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SX60])

HSP 1 Score: 175.637 bits (444), Expect = 3.969e-56
Identity = 81/139 (58.27%), Postives = 107/139 (76.98%), Query Frame = 3 
Query:   90 MWFQKEIILDKYSRGCHYITDVVIKTLPEIKNYKIGILHLFMKHTSASLTLNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHSVSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQGT 506
            +W Q  I ++   RG H ITD + K +PE+K YKIG+ HL ++HTSASL+LNE++D  V+ DMEMMLN++ PE+  Y H+ EGPDDMPAHVK SL+G  S+++PI++G LN+GTWQGIW CEHR+H S R II T+QGT
Sbjct:    8 VWHQTSITVEPKKRGIHLITDKINK-IPELKKYKIGLAHLLLQHTSASLSLNESWDPSVRKDMEMMLNRLAPEDAPYIHTLEGPDDMPAHVKTSLMGT-SLTVPITNGKLNIGTWQGIWLCEHRDHASARHIIITLQGT 144
[Close]
BLAST of Eukaryotic translation initiation factor 3 subunit C vs. Planmine SMEST
Match: SMESG000061315.1 (SMESG000061315.1)

HSP 1 Score: 292.738 bits (748), Expect = 4.241e-102
Identity = 139/140 (99.29%), Postives = 140/140 (100.00%), Query Frame = 3 
Query:   90 MWFQKEIILDKYSRGCHYITDVVIKTLPEIKNYKIGILHLFMKHTSASLTLNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHSVSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQGTY 509
            MWFQKEIILDKYSRGCHYITDVVIK+LPEIKNYKIGILHLFMKHTSASLTLNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHSVSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQGTY
Sbjct:    1 MWFQKEIILDKYSRGCHYITDVVIKSLPEIKNYKIGILHLFMKHTSASLTLNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHSVSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQGTY 140
The following BLAST results are available for this feature:
BLAST of Eukaryotic translation initiation factor 3 subunit C vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic translation initiation factor 3 subunit C vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic translation initiation factor 3 subunit C vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG316884.193e-5655.47gene:FBgn0263355 transcript:FBtr0308895[more]
CG316884.193e-5655.47gene:FBgn0263355 transcript:FBtr0273378[more]
CG316884.193e-5655.47gene:FBgn0263355 transcript:FBtr0330661[more]
CG316884.210e-5655.47gene:FBgn0263355 transcript:FBtr0330203[more]
CG316882.169e-5255.47gene:FBgn0263355 transcript:FBtr0330204[more]
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BLAST of Eukaryotic translation initiation factor 3 subunit C vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic translation initiation factor 3 subunit C vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic translation initiation factor 3 subunit C vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic translation initiation factor 3 subunit C vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P0AF48|YJBQ_ECOLI5.428e-4651.45UPF0047 protein YjbQ OS=Escherichia coli (strain K... [more]
sp|P0AF49|YJBQ_ECO575.428e-4651.45UPF0047 protein YjbQ OS=Escherichia coli O157:H7 O... [more]
sp|O14155|YE72_SCHPO3.503e-4349.26UPF0047 protein C4A8.02c OS=Schizosaccharomyces po... [more]
sp|P0A2L1|YJBQ_SALTY9.086e-4052.90UPF0047 protein YjbQ OS=Salmonella typhimurium (st... [more]
sp|P0A2L2|YJBQ_SALTI9.086e-4052.90UPF0047 protein YjbQ OS=Salmonella typhi OX=90370 ... [more]
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BLAST of Eukaryotic translation initiation factor 3 subunit C vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0X3Q0H32.876e-6667.39UPF0047 protein YjbQ (Fragment) OS=Schistocephalus... [more]
A0A564YXM43.737e-6564.49Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
A0A068YIQ61.562e-6464.49Expressed conserved protein OS=Echinococcus multil... [more]
A0A068WF161.562e-6464.49Expressed conserved protein OS=Echinococcus granul... [more]
A0A5K3F6091.151e-6365.22Uncharacterized protein OS=Mesocestoides corti OX=... [more]
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BLAST of Eukaryotic translation initiation factor 3 subunit C vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic translation initiation factor 3 subunit C vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic translation initiation factor 3 subunit C vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic translation initiation factor 3 subunit C vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO317173.969e-5658.27Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Eukaryotic translation initiation factor 3 subunit C vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic translation initiation factor 3 subunit C vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000061315.14.241e-10299.29SMESG000061315.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30026999 ID=SMED30026999|Name=Eukaryotic translation initiation factor 3 subunit C|organism=Schmidtea mediterranea sexual|type=transcript|length=732bp
AGTGAACAATTTAAATTAATGTTTTTATTAAAATTATTTTTCTGTAATTT
GTAGTTTTATAAATTTACATTTTATATTTATATGTAATAATGTGGTTTCA
GAAAGAAATTATTTTAGACAAATATTCTAGAGGTTGCCATTACATAACTG
ATGTAGTCATCAAAACATTACCAGAAATCAAAAATTATAAAATTGGAATA
TTGCATTTGTTCATGAAACATACTTCTGCAAGTCTAACATTAAATGAAAA
TTATGATAGTGATGTCAAGGTTGATATGGAAATGATGCTGAACAAAATTG
TCCCCGAGAATTTAAATTATAAACATAGTTGTGAAGGCCCAGATGATATG
CCAGCCCATGTAAAACATTCACTTCTTGGTGGGCATAGTGTTAGTATGCC
GATATCAGATGGAACTCTAAATCTCGGTACGTGGCAAGGAATATGGTTTT
GTGAACATAGGAACCATAAATCCCAGCGCAAAATCATAGCTACTATCCAA
GGAACCTATTAGTCGATTGTTAATTTGAATATGAAAATGTCGTCATTATT
AAATAATTATATGAACAAAATATTTCTTTTATGGCGCTTAAATGTAATGA
ATTTTAATGTTTAACGCTTTTATATAATATTCTAAATATTAAAAATTTGA
ATAAAAAAATAATATTCACTTCATTTGTAACTTATCAAATCCGTTTTTAC
AAGAAATGTGGTAATTGAAATTGACAGAGTTA
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protein sequence of SMED30026999-orf-1

>SMED30026999-orf-1 ID=SMED30026999-orf-1|Name=SMED30026999-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=141bp
MWFQKEIILDKYSRGCHYITDVVIKTLPEIKNYKIGILHLFMKHTSASLT
LNENYDSDVKVDMEMMLNKIVPENLNYKHSCEGPDDMPAHVKHSLLGGHS
VSMPISDGTLNLGTWQGIWFCEHRNHKSQRKIIATIQGTY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0001732formation of cytoplasmic translation initiation complex
GO:0002183cytoplasmic translational initiation
GO:0001731formation of translation preinitiation complex
GO:0006412translation
GO:0006413translational initiation
GO:0006446regulation of translational initiation
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR035917YjbQ-like_sf
IPR001602UPF0047
Vocabulary: molecular function
TermDefinition
GO:0003723RNA binding
GO:0003743translation initiation factor activity
GO:0031369translation initiation factor binding
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
GO:0005852eukaryotic translation initiation factor 3 complex
GO:0016282eukaryotic 43S preinitiation complex
GO:0033290eukaryotic 48S preinitiation complex
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001602Uncharacterised protein family UPF0047PIRSFPIRSF004681UCP004681coord: 1..139
e-value: 4.6E-45
score: 150.3
IPR001602Uncharacterised protein family UPF0047PFAMPF01894UPF0047coord: 19..135
e-value: 2.4E-37
score: 127.6
IPR001602Uncharacterised protein family UPF0047TIGRFAMTIGR00149TIGR00149coord: 11..138
e-value: 6.8E-41
score: 137.1
IPR001602Uncharacterised protein family UPF0047PANTHERPTHR30615UNCHARACTERIZED PROTEIN YJBQ-RELATEDcoord: 1..138
IPR035917YjbQ-like superfamilyGENE3DG3DSA:2.60.120.460coord: 2..139
e-value: 7.2E-41
score: 141.1
IPR035917YjbQ-like superfamilySUPERFAMILYSSF111038YjbQ-likecoord: 1..138
NoneNo IPR availablePANTHERPTHR30615:SF8SECONDARY THIAMINE-PHOSPHATE SYNTHASE ENZYMEcoord: 1..138