Serine/threonine kinase
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30026997 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of Serine/threonine kinase vs. Ensembl Human
Match: CHEK2 (checkpoint kinase 2 [Source:HGNC Symbol;Acc:HGNC:16627]) HSP 1 Score: 335.88 bits (860), Expect = 7.239e-109 Identity = 192/440 (43.64%), Postives = 269/440 (61.14%), Query Frame = 2
Query: 83 WGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSLCPY------------FSSLSKIHFEIFKE-----NQNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLE-STQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQ----GVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSF-PEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYPLSEVTKLCQ 1333
W + L F +E + +Y GR + C Y + + SK HF IF+E + + D S NGT+VN VGK K L +N I+L+ +NK FVF L Q +YP +R++Y +S+TLG GACGEVKLA+ T +KVAIKII K+ + S + VE E+EIL++LNHPCI+K+++ D E +IV+EL+EGGELFD+V+ K+ +E ++ YQM++A++ YLH+N I HRDLKPEN+LL++ + LIK+TDFG SK + ++M+TLCGTP YLAPEV++ GT Y+ A D WSLGVILFICL+GYPPF+E RT + L QIT G+Y+F PEV W+ VS A+ L+K++L VDPK R T + L+HPW+ D+ +K ++ +E T L Q
Sbjct: 93 WARLWALQDGFANLECVNDNYWFGR------DKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVKLAFERKTCKKVAIKIISKRKFAIGSAREADPALNVETEIEILKKLNHPCIIKIKNFFDA-EDYYIVLELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAVQ--------------YLHENGIIHRDLKPENVLLSSQEEDCLIKITDFGHSKILGETSLMRTLCGTPTYLAPEVLVSVGTAGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVSLKDQITSGKYNFIPEV-WAEVSEKALDLVKKLLVVDPKARFTTEEALRHPWLQDEDMKRKFQDLLSEENESTALPQ 510
BLAST of Serine/threonine kinase vs. Ensembl Human
Match: CHEK2 (checkpoint kinase 2 [Source:HGNC Symbol;Acc:HGNC:16627]) HSP 1 Score: 335.88 bits (860), Expect = 7.239e-109 Identity = 192/440 (43.64%), Postives = 269/440 (61.14%), Query Frame = 2
Query: 83 WGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSLCPY------------FSSLSKIHFEIFKE-----NQNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLE-STQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQ----GVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSF-PEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYPLSEVTKLCQ 1333
W + L F +E + +Y GR + C Y + + SK HF IF+E + + D S NGT+VN VGK K L +N I+L+ +NK FVF L Q +YP +R++Y +S+TLG GACGEVKLA+ T +KVAIKII K+ + S + VE E+EIL++LNHPCI+K+++ D E +IV+EL+EGGELFD+V+ K+ +E ++ YQM++A++ YLH+N I HRDLKPEN+LL++ + LIK+TDFG SK + ++M+TLCGTP YLAPEV++ GT Y+ A D WSLGVILFICL+GYPPF+E RT + L QIT G+Y+F PEV W+ VS A+ L+K++L VDPK R T + L+HPW+ D+ +K ++ +E T L Q
Sbjct: 93 WARLWALQDGFANLECVNDNYWFGR------DKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVKLAFERKTCKKVAIKIISKRKFAIGSAREADPALNVETEIEILKKLNHPCIIKIKNFFDA-EDYYIVLELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAVQ--------------YLHENGIIHRDLKPENVLLSSQEEDCLIKITDFGHSKILGETSLMRTLCGTPTYLAPEVLVSVGTAGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVSLKDQITSGKYNFIPEV-WAEVSEKALDLVKKLLVVDPKARFTTEEALRHPWLQDEDMKRKFQDLLSEENESTALPQ 510
BLAST of Serine/threonine kinase vs. Ensembl Human
Match: CHEK2 (checkpoint kinase 2 [Source:HGNC Symbol;Acc:HGNC:16627]) HSP 1 Score: 335.88 bits (860), Expect = 7.239e-109 Identity = 192/440 (43.64%), Postives = 269/440 (61.14%), Query Frame = 2
Query: 83 WGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSLCPY------------FSSLSKIHFEIFKE-----NQNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLE-STQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQ----GVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSF-PEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYPLSEVTKLCQ 1333
W + L F +E + +Y GR + C Y + + SK HF IF+E + + D S NGT+VN VGK K L +N I+L+ +NK FVF L Q +YP +R++Y +S+TLG GACGEVKLA+ T +KVAIKII K+ + S + VE E+EIL++LNHPCI+K+++ D E +IV+EL+EGGELFD+V+ K+ +E ++ YQM++A++ YLH+N I HRDLKPEN+LL++ + LIK+TDFG SK + ++M+TLCGTP YLAPEV++ GT Y+ A D WSLGVILFICL+GYPPF+E RT + L QIT G+Y+F PEV W+ VS A+ L+K++L VDPK R T + L+HPW+ D+ +K ++ +E T L Q
Sbjct: 93 WARLWALQDGFANLECVNDNYWFGR------DKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVKLAFERKTCKKVAIKIISKRKFAIGSAREADPALNVETEIEILKKLNHPCIIKIKNFFDA-EDYYIVLELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAVQ--------------YLHENGIIHRDLKPENVLLSSQEEDCLIKITDFGHSKILGETSLMRTLCGTPTYLAPEVLVSVGTAGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVSLKDQITSGKYNFIPEV-WAEVSEKALDLVKKLLVVDPKARFTTEEALRHPWLQDEDMKRKFQDLLSEENESTALPQ 510
BLAST of Serine/threonine kinase vs. Ensembl Human
Match: CHEK2 (checkpoint kinase 2 [Source:HGNC Symbol;Acc:HGNC:16627]) HSP 1 Score: 331.257 bits (848), Expect = 1.553e-106 Identity = 183/391 (46.80%), Postives = 255/391 (65.22%), Query Frame = 2
Query: 194 FSSLSKIHFEIFKE-----NQNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLE-STQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQ----GVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSF-PEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYPLSEVTKLCQ 1333
+ + SK HF IF+E + + D S NGT+VN VGK K L +N I+L+ +NK FVF L Q +YP +R++Y +S+TLG GACGEVKLA+ T +KVAIKII K+ + S + VE E+EIL++LNHPCI+K+++ D E +IV+EL+EGGELFD+V+ K+ +E ++ YQM++A++ YLH+N I HRDLKPEN+LL++ + LIK+TDFG SK + ++M+TLCGTP YLAPEV++ GT Y+ A D WSLGVILFICL+GYPPF+E RT + L QIT G+Y+F PEV W+ VS A+ L+K++L VDPK R T + L+HPW+ D+ +K ++ +E T L Q
Sbjct: 179 YRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVKLAFERKTCKKVAIKIISKRKFAIGSAREADPALNVETEIEILKKLNHPCIIKIKNFFDA-EDYYIVLELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAVQ--------------YLHENGIIHRDLKPENVLLSSQEEDCLIKITDFGHSKILGETSLMRTLCGTPTYLAPEVLVSVGTAGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVSLKDQITSGKYNFIPEV-WAEVSEKALDLVKKLLVVDPKARFTTEEALRHPWLQDEDMKRKFQDLLSEENESTALPQ 553
BLAST of Serine/threonine kinase vs. Ensembl Human
Match: CHEK2 (checkpoint kinase 2 [Source:HGNC Symbol;Acc:HGNC:16627]) HSP 1 Score: 295.434 bits (755), Expect = 2.656e-94 Identity = 160/331 (48.34%), Postives = 222/331 (67.07%), Query Frame = 2
Query: 359 NKAFVFMHLE-STQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQ----GVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSF-PEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYPLSEVTKLCQ 1333
N FVF L Q +YP +R++Y +S+TLG GACGEVKLA+ T +KVAIKII K+ + S + VE E+EIL++LNHPCI+K+++ D E +IV+EL+EGGELFD+V+ K+ +E ++ YQM++A++ YLH+N I HRDLKPEN+LL++ + LIK+TDFG SK + ++M+TLCGTP YLAPEV++ GT Y+ A D WSLGVILFICL+GYPPF+E RT + L QIT G+Y+F PEV W+ VS A+ L+K++L VDPK R T + L+HPW+ D+ +K ++ +E T L Q
Sbjct: 105 NLVFVFFDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVKLAFERKTCKKVAIKIISKRKFAIGSAREADPALNVETEIEILKKLNHPCIIKIKNFFDA-EDYYIVLELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAVQ--------------YLHENGIIHRDLKPENVLLSSQEEDCLIKITDFGHSKILGETSLMRTLCGTPTYLAPEVLVSVGTAGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVSLKDQITSGKYNFIPEV-WAEVSEKALDLVKKLLVVDPKARFTTEEALRHPWLQDEDMKRKFQDLLSEENESTALPQ 419
BLAST of Serine/threonine kinase vs. Ensembl Celegans
Match: chk-2 (Serine/threonine-protein kinase chk-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U1Y5]) HSP 1 Score: 210.69 bits (535), Expect = 4.863e-62 Identity = 139/454 (30.62%), Postives = 223/454 (49.12%), Query Frame = 2
Query: 14 EDSQFNTQELFSQNDAEIINSNAWGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSL--------CPYFSSLSKIHFEIFK--ENQNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLESTQHLYPMVVRNKYTI-SRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXS---TKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQ----FEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYPLSEVT 1321
D E +++ S + G+ ++L D + GRG + + +SKI F I + E + + L D S+NGT VN +GK + L + D+IS+ FV+ ++ H P + KY + S +LGKG G+V L Y VAIK ++ + S K++ + EVE++++L+HP IV + D I + ++V+E V GGE F +V+ K E + +Q++ D I YLH I HRD+KPENIL + ++K+TDFG++K + MKT CGTP+Y APE++ G EY+ DIWSLG +LFI +GYPPF+EE TD+ + +Q+ G+ F W ++ ++IK ML V+P R + ++++ WM +T I+ + ++
Sbjct: 23 RDDTMPVDEDLVVGESQCAASKPFAKLVGVRRGISSIDLADDHFVCGRGSDDAPTNFNFSQVAKDVGLYRFISKIQFSIDRDTETRRIYLHDHSRNGTLVNQEMIGKGLSRELMNGDLISIGIPALIIFVYESADADHH--PEELTKKYHVTSHSLGKGGFGKVLLGYKKSDRSVVAIKQLNTQFSTRCSRAIAKTRDIRNEVEVMKKLSHPNIVAIYDWITVAKYSYMVIEYVGGGEFFSKVVDSKYNRMGLGESLGKYFAFQLI--------------DAILYLHSVGICHRDIKPENILCSDKAERCILKLTDFGMAK--NSVNRMKTRCGTPSYNAPEIVANEGV-EYTPKVDIWSLGCVLFITFSGYPPFSEEYTDMTMDEQVLTGRLIF-HAQWRRITVETQNMIKWMLTVEPSNRPSAVELMSTQWMKCADCRTAKQDILKSIKPIS 456
BLAST of Serine/threonine kinase vs. Ensembl Celegans
Match: T08D2.7 (pep chromosome:WBcel235:X:182691:191193:1 gene:WBGene00011612.1 transcript:T08D2.7.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T08D2.7) HSP 1 Score: 209.149 bits (531), Expect = 1.785e-61 Identity = 135/411 (32.85%), Postives = 213/411 (51.82%), Query Frame = 2
Query: 83 WGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSL--------CPYFSSLSKIHFEIFK--ENQNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLESTQHLYPMVVRNKYTI-SRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXS---TKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFE----EVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWM 1261
+ G+ ++L D + GRG + + +SKI F I + E + + L D S+NGT VN +GK + L + D+IS+A FV+ + H P + NKY + S LGKG G+V L Y VAIK ++ + S K++ ++ EVE++++L+HP IV + D I + ++V+E V GGE F +++ K + E + +Q++ D + YLH I HRD+KPENIL + ++K+TDFG++K + MKT CGTP+Y APE++ G EY+ DIWSLG +LFI +GYPPF+EE TDL + +Q+ G+ F E W ++ ++I+ ML V+P R + ++++ WM
Sbjct: 45 FAKLVGVRRGISSIDLADDHFVCGRGSHDAPTNFNFSTVAEDVGLYKFISKIQFSIDRDTETRRIYLHDHSRNGTLVNHEMIGKGLSRELMNGDLISIAIPALIIFVYERADDNHH--PEELTNKYHVTSHLLGKGGFGKVLLGYKKSDRSVVAIKQLNTQFSTRCSRAIAKTRDIQNEVEVMKKLSHPNIVAIYDCITVAKYSYMVIEYVGGGEFFSKLVDSKYNQMGLGESLGKYFAFQLI--------------DAVLYLHSVGICHRDIKPENILCSNKAERCILKLTDFGMAK--NSVNRMKTHCGTPSYCAPEIVANQGV-EYTPKVDIWSLGCVLFITFSGYPPFSEEYTDLKMDEQVLTGRLFFHE-QWHRITDETQNMIQWMLTVEPLIRPSAVELMSTQWM 435
BLAST of Serine/threonine kinase vs. Ensembl Celegans
Match: cmk-1 (Calcium/calmodulin-dependent protein kinase type 1 [Source:UniProtKB/Swiss-Prot;Acc:Q9TXJ0]) HSP 1 Score: 193.741 bits (491), Expect = 6.478e-57 Identity = 108/290 (37.24%), Postives = 158/290 (54.48%), Query Frame = 2
Query: 413 VVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKT 1282
+R KY LG GA +V LA + + + K K + +E E+++LR+L H IV+L D D + +++VMELV GGELFDR+++ + E DA L+ Q++ A+ ++HDN + HRDLKPEN+L + I ++DFGLSK D +M T CGTP Y+APEV+ + Y A D+WS+GVI +I L GYPPF +E +D L QI +G+Y F +W +S A I ++ DP+ R T L HPW++ + T
Sbjct: 17 TIREKYDFRDVLGTGAFSKVFLA--ESKSDAGQMYAVKCIDKKALKGKEESLENEIKVLRKLRHNNIVQLFDTYDEKQFVYLVMELVTGGELFDRIVAKGSYTEQDASNLIRQVLEAVG--------------FMHDNGVVHRDLKPENLLYYNQDEDSKIMISDFGLSKTEDSG-VMATACGTPGYVAPEVL---QQKPYGKAVDVWSIGVIAYILLCGYPPFYDE-SDANLFAQIIKGEYEFDAPYWDQISDSAKDFITHLMCCDPEARFTCQDALSHPWISGNTAYT 285
BLAST of Serine/threonine kinase vs. Ensembl Celegans
Match: unc-43 (Calcium/calmodulin-dependent protein kinase type II [Source:UniProtKB/Swiss-Prot;Acc:O62305]) HSP 1 Score: 181.8 bits (460), Expect = 5.120e-53 Identity = 109/289 (37.72%), Postives = 156/289 (53.98%), Query Frame = 2
Query: 422 NKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWM-NDDKVKTT 1285
+ Y + LGKGA V+ TG + KL++ Q +ERE I R+L HP IV+L D+I + ++V +LV GGELF+ +++ + + E DA + Q++ ++I Y H N I HRDLKPEN+LLA+ +K+ DFGL+ V+ + GTP YL+PEV+ + YS DIW+ GVIL+I L GYPPF +E L QI G Y +P W V+ A LI ML V+PKKRIT + L+ PW+ N ++V +
Sbjct: 10 DNYDVKEELGKGAFSVVRRCVHKTTG--LEFAAKIINTKKLSARDFQKLEREARICRKLQHPNIVRLHDSIQEESFHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQIL--------------ESIAYCHSNGIVHRDLKPENLLLASKAKGAAVKLADFGLAIEVNDSEAWHGFAGTPGYLSPEVLKKD---PYSKPVDIWACGVILYILLVGYPPFWDEDQHR-LYAQIKAGAYDYPSPEWDTVTPEAKSLIDSMLTVNPKKRITADQALKVPWICNRERVASA 278
BLAST of Serine/threonine kinase vs. Ensembl Celegans
Match: unc-43 (Calcium/calmodulin-dependent protein kinase type II [Source:UniProtKB/Swiss-Prot;Acc:O62305]) HSP 1 Score: 181.8 bits (460), Expect = 5.120e-53 Identity = 109/289 (37.72%), Postives = 156/289 (53.98%), Query Frame = 2
Query: 422 NKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWM-NDDKVKTT 1285
+ Y + LGKGA V+ TG + KL++ Q +ERE I R+L HP IV+L D+I + ++V +LV GGELF+ +++ + + E DA + Q++ ++I Y H N I HRDLKPEN+LLA+ +K+ DFGL+ V+ + GTP YL+PEV+ + YS DIW+ GVIL+I L GYPPF +E L QI G Y +P W V+ A LI ML V+PKKRIT + L+ PW+ N ++V +
Sbjct: 10 DNYDVKEELGKGAFSVVRRCVHKTTG--LEFAAKIINTKKLSARDFQKLEREARICRKLQHPNIVRLHDSIQEESFHYLVFDLVTGGELFEDIVAREFYSEADASHCIQQIL--------------ESIAYCHSNGIVHRDLKPENLLLASKAKGAAVKLADFGLAIEVNDSEAWHGFAGTPGYLSPEVLKKD---PYSKPVDIWACGVILYILLVGYPPFWDEDQHR-LYAQIKAGAYDYPSPEWDTVTPEAKSLIDSMLTVNPKKRITADQALKVPWICNRERVASA 278
BLAST of Serine/threonine kinase vs. Ensembl Fly
Match: lok (gene:FBgn0019686 transcript:FBtr0081329) HSP 1 Score: 292.352 bits (747), Expect = 2.208e-93 Identity = 162/444 (36.49%), Postives = 261/444 (58.78%), Query Frame = 2
Query: 14 EDSQFNTQELFSQNDAEIINSNAWGAFFGLLGKFPCVELTDSSYTVGRGGK----IILNSLC-PYFSSLSKIHFEIFKEN----QNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLESTQHL-YPMVVRNKYTISRTLGKGACGEVKLAYVNGTGE--XXXXXXXXXXXXXXXSTKSQGVER---EVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIM 1300
+ +Q +++Q +++ + WG +G K ++L + +T GRG + LN L + +SK+HF I + N V ++DLS+NGT+VN+ K+G ++ +L+++DVISL+ KAFVF L + + P + Y ++R LG GA G V+L Y T + + I + + ST +R E +I++ L+HPC+V++ D +D + +++V+E + GG+L +R+IS K E +++ YQM A+K YLHD I+HRDLKP+N+LL T+ TL+KV+DFGLSKFV ++M+TLCGTP Y+APEV++ G + Y+ DIWSLGV+LF CL+G PF++E P ++QI +G++++ W VS+ A LI QML VDP++R ++ +LQ W+ D + A ++M
Sbjct: 9 QGTQSQASNIWTQVESQPMEKIVWGRLYGKNIKIKSLDLNNDEFTAGRGEANDLILTLNDLPEKILTRISKVHFIIKRANCELTNPVYIQDLSRNGTFVNNEKIGTNRMRILKNDDVISLSHPTYKAFVFKDLSPNESIGLPEEINKTYYVNRKLGSGAYGLVRLVYDTRTCQQFAMKIVKKNMLSGARPSTNFSDPDRVLNEAKIMKNLSHPCVVRMHDIVDKPDSVYMVLEFMRGGDLLNRIISNKLLSEDISKLYFYQMCHAVK--------------YLHDRGITHRDLKPDNVLLETNDEETLLKVSDFGLSKFVQKDSVMRTLCGTPLYVAPEVLITGGREAYTKKVDIWSLGVVLFTCLSGTLPFSDE-YGTPAAQQIKKGRFAYGHPSWKSVSQRAKLLINQMLIVDPERRPSIDDVLQSSWLRDAPMLQKAKRLM 437
BLAST of Serine/threonine kinase vs. Ensembl Fly
Match: lok (gene:FBgn0019686 transcript:FBtr0081330) HSP 1 Score: 292.352 bits (747), Expect = 2.208e-93 Identity = 162/444 (36.49%), Postives = 261/444 (58.78%), Query Frame = 2
Query: 14 EDSQFNTQELFSQNDAEIINSNAWGAFFGLLGKFPCVELTDSSYTVGRGGK----IILNSLC-PYFSSLSKIHFEIFKEN----QNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLESTQHL-YPMVVRNKYTISRTLGKGACGEVKLAYVNGTGE--XXXXXXXXXXXXXXXSTKSQGVER---EVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIM 1300
+ +Q +++Q +++ + WG +G K ++L + +T GRG + LN L + +SK+HF I + N V ++DLS+NGT+VN+ K+G ++ +L+++DVISL+ KAFVF L + + P + Y ++R LG GA G V+L Y T + + I + + ST +R E +I++ L+HPC+V++ D +D + +++V+E + GG+L +R+IS K E +++ YQM A+K YLHD I+HRDLKP+N+LL T+ TL+KV+DFGLSKFV ++M+TLCGTP Y+APEV++ G + Y+ DIWSLGV+LF CL+G PF++E P ++QI +G++++ W VS+ A LI QML VDP++R ++ +LQ W+ D + A ++M
Sbjct: 9 QGTQSQASNIWTQVESQPMEKIVWGRLYGKNIKIKSLDLNNDEFTAGRGEANDLILTLNDLPEKILTRISKVHFIIKRANCELTNPVYIQDLSRNGTFVNNEKIGTNRMRILKNDDVISLSHPTYKAFVFKDLSPNESIGLPEEINKTYYVNRKLGSGAYGLVRLVYDTRTCQQFAMKIVKKNMLSGARPSTNFSDPDRVLNEAKIMKNLSHPCVVRMHDIVDKPDSVYMVLEFMRGGDLLNRIISNKLLSEDISKLYFYQMCHAVK--------------YLHDRGITHRDLKPDNVLLETNDEETLLKVSDFGLSKFVQKDSVMRTLCGTPLYVAPEVLITGGREAYTKKVDIWSLGVVLFTCLSGTLPFSDE-YGTPAAQQIKKGRFAYGHPSWKSVSQRAKLLINQMLIVDPERRPSIDDVLQSSWLRDAPMLQKAKRLM 437
BLAST of Serine/threonine kinase vs. Ensembl Fly
Match: lok (gene:FBgn0019686 transcript:FBtr0081328) HSP 1 Score: 283.108 bits (723), Expect = 1.353e-89 Identity = 163/461 (35.36%), Postives = 261/461 (56.62%), Query Frame = 2
Query: 14 EDSQFNTQELFSQNDAEIINSNAWGAFFGLLGKFPC-----------------VELTDSSYTVGRGGK----IILNSLC-PYFSSLSKIHFEIFKEN----QNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLESTQHL-YPMVVRNKYTISRTLGKGACGEVKLAYVNGTGE--XXXXXXXXXXXXXXXSTKSQGVER---EVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIM 1300
+ +Q +++Q +++ + WG +G K V+L + +T GRG + LN L + +SK+HF I + N V ++DLS+NGT+VN+ K+G ++ +L+++DVISL+ KAFVF L + + P + Y ++R LG GA G V+L Y T + + I + + ST +R E +I++ L+HPC+V++ D +D + +++V+E + GG+L +R+IS K E +++ YQM A+K YLHD I+HRDLKP+N+LL T+ TL+KV+DFGLSKFV ++M+TLCGTP Y+APEV++ G + Y+ DIWSLGV+LF CL+G PF++E P ++QI +G++++ W VS+ A LI QML VDP++R ++ +LQ W+ D + A ++M
Sbjct: 9 QGTQSQASNIWTQVESQPMEKIVWGRLYGKNIKIKSLGTSSKYRIIYTHSSFSVDLNNDEFTAGRGEANDLILTLNDLPEKILTRISKVHFIIKRANCELTNPVYIQDLSRNGTFVNNEKIGTNRMRILKNDDVISLSHPTYKAFVFKDLSPNESIGLPEEINKTYYVNRKLGSGAYGLVRLVYDTRTCQQFAMKIVKKNMLSGARPSTNFSDPDRVLNEAKIMKNLSHPCVVRMHDIVDKPDSVYMVLEFMRGGDLLNRIISNKLLSEDISKLYFYQMCHAVK--------------YLHDRGITHRDLKPDNVLLETNDEETLLKVSDFGLSKFVQKDSVMRTLCGTPLYVAPEVLITGGREAYTKKVDIWSLGVVLFTCLSGTLPFSDE-YGTPAAQQIKKGRFAYGHPSWKSVSQRAKLLINQMLIVDPERRPSIDDVLQSSWLRDAPMLQKAKRLM 454
BLAST of Serine/threonine kinase vs. Ensembl Fly
Match: CaMKI (gene:FBgn0016126 transcript:FBtr0333670) HSP 1 Score: 192.971 bits (489), Expect = 4.470e-56 Identity = 115/290 (39.66%), Postives = 169/290 (58.28%), Query Frame = 2
Query: 416 VRNKYTISRTLGKGACGEVKLAYVNGT-GEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKT 1282
+ KY + LG GA EV+LA + GE A+KIIDKKAL K + +E E+ +LR+L HP IV+L + + ++++VMELV GGELFDR++ + E DA L+ Q++ A+ Y+H+ + HRDLKPEN+L + + + I ++DFGLSK D +M T CGTP Y+APEV+ + Y A D+WS+GVI +I L GYPPF +E D L QI +G + F +W +S A H IK ++ V +KR T + L H W++ ++ +
Sbjct: 27 IEEKYNLHGLLGTGAFSEVRLAESKDSPGEHFAVKIIDKKAL---KGKEESLENEIRVLRRLTHPNIVQLLETYEDKSKVYLVMELVTGGELFDRIVEKGSYTEKDASHLIRQILEAVD--------------YMHEQGVVHRDLKPENLLYYSPDDDSKIMISDFGLSKMEDSG-IMATACGTPGYVAPEVL---AQKPYGKAVDVWSIGVISYILLCGYPPFYDE-NDANLFAQILKGDFEFDSPYWDEISESAKHFIKNLMCVTVEKRYTCKQALGHAWISGNEASS 294
BLAST of Serine/threonine kinase vs. Ensembl Fly
Match: CaMKI (gene:FBgn0016126 transcript:FBtr0089067) HSP 1 Score: 182.956 bits (463), Expect = 3.318e-52 Identity = 115/305 (37.70%), Postives = 169/305 (55.41%), Query Frame = 2
Query: 416 VRNKYTISRTLGKGACGEVKLAYVNGT-GEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQ---------------LNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKT 1282
+ KY + LG GA EV+LA + GE A+KIIDKKAL K + +E E+ +LR+ L HP IV+L + + ++++VMELV GGELFDR++ + E DA L+ Q++ A+ Y+H+ + HRDLKPEN+L + + + I ++DFGLSK D +M T CGTP Y+APEV+ + Y A D+WS+GVI +I L GYPPF +E D L QI +G + F +W +S A H IK ++ V +KR T + L H W++ ++ +
Sbjct: 27 IEEKYNLHGLLGTGAFSEVRLAESKDSPGEHFAVKIIDKKAL---KGKEESLENEIRVLRRFSANHFDGKCLNGTRLTHPNIVQLLETYEDKSKVYLVMELVTGGELFDRIVEKGSYTEKDASHLIRQILEAVD--------------YMHEQGVVHRDLKPENLLYYSPDDDSKIMISDFGLSKMEDSG-IMATACGTPGYVAPEVL---AQKPYGKAVDVWSIGVISYILLCGYPPFYDE-NDANLFAQILKGDFEFDSPYWDEISESAKHFIKNLMCVTVEKRYTCKQALGHAWISGNEASS 309
BLAST of Serine/threonine kinase vs. Ensembl Zebrafish
Match: chek2 (checkpoint kinase 2 [Source:NCBI gene;Acc:336998]) HSP 1 Score: 348.977 bits (894), Expect = 1.542e-114 Identity = 195/471 (41.40%), Postives = 279/471 (59.24%), Query Frame = 2
Query: 35 QELFS-QNDAEIINSNAWGAFFGLLGKFPCVELTDSSYTVGRGGKI---ILNSL---CPYFSSLSKIHFEIFKENQNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFM-HLESTQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYPLSEVTKLCQEDLGPCSPSAVGTVNENDNSLSGEPVVKRR 1423
QEL S D E + WG L F + T++ Y+ GR + NS+ PYF++ SK HF IF++ V L DLS NGT+V+D K+G K LL +N VI+LA QK++ F+F+ + Q P+ KY I+R +G G CGEVKLA T +KVA+K I+K T ++ ERE+EIL++++HPC++K D T++ +IV+E +EGGELF R+ + K+ EE A++ YQM+ A++ YLH+N I HRDLKPEN+LLA+ ++ LIK+TDF SK ++ +++MKTLCGTP Y+APEV T Y+ A D WSLGV+LFICL GYPPF E T + + +QI G+Y F W VS A L+K++L VDP+KR+++ + L+HPW+ DD+++ A ++M P + P P A T GEP KR+
Sbjct: 54 QELQSIPEDEEEVQPQVWGRIIPLKQGFSVLNCTENQYSFGRDKRCDYSFSNSILKKSPYFNTYSKKHFRIFRDENLVYLEDLSGNGTWVDDEKLGNGKQSLLSNNSVIALAEQKHQVFMFIDKMADDQANLPLEFSKKYHIARKIGTGVCGEVKLAIEKETFKKVALKTINKHDFPSIGTATRNAEREIEILKKIDHPCLIKTEDFYQTEDSYYIVLEYIEGGELFGRIKAKKKLEEDIAKLYFYQMLKAVE--------------YLHNNGIIHRDLKPENVLLASHDDICLIKITDFNQSKILEESSLMKTLCGTPTYVAPEVFTHASTVGYTKAVDYWSLGVLLFICLGGYPPFNTECTTMSVREQIINGEYRFIPSQWKNVSNEAKDLVKKLLVVDPQKRLSVEEALEHPWLKDDRMRQNANQLMNPGAANQ--------PMRPEA--TRKRKAQEGEGEPASKRK 500
BLAST of Serine/threonine kinase vs. Ensembl Zebrafish
Match: chek2 (checkpoint kinase 2 [Source:NCBI gene;Acc:336998]) HSP 1 Score: 348.206 bits (892), Expect = 2.560e-114 Identity = 195/471 (41.40%), Postives = 279/471 (59.24%), Query Frame = 2
Query: 35 QELFS-QNDAEIINSNAWGAFFGLLGKFPCVELTDSSYTVGRGGKI---ILNSL---CPYFSSLSKIHFEIFKENQNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFM-HLESTQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYPLSEVTKLCQEDLGPCSPSAVGTVNENDNSLSGEPVVKRR 1423
QEL S D E + WG L F + T++ Y+ GR + NS+ PYF++ SK HF IF++ V L DLS NGT+V+D K+G K LL +N VI+LA QK++ F+F+ + Q P+ KY I+R +G G CGEVKLA T +KVA+K I+K T ++ ERE+EIL++++HPC++K D T++ +IV+E +EGGELF R+ + K+ EE A++ YQM+ A++ YLH+N I HRDLKPEN+LLA+ ++ LIK+TDF SK ++ +++MKTLCGTP Y+APEV T Y+ A D WSLGV+LFICL GYPPF E T + + +QI G+Y F W VS A L+K++L VDP+KR+++ + L+HPW+ DD+++ A ++M P + P P A T GEP KR+
Sbjct: 54 QELQSIPEDEEEVQPQVWGRIIPLKQGFSVLNCTENQYSFGRDKRCDYSFSNSILKKSPYFNTYSKKHFRIFRDENLVYLEDLSGNGTWVDDEKLGNGKQSLLSNNSVIALAEQKHQVFMFIDKMADDQANLPLEFSKKYHIARKIGTGVCGEVKLAIEKETFKKVALKTINKHDFPSIGTATRNAEREIEILKKIDHPCLIKTEDFYQTEDSYYIVLEYIEGGELFGRIKAKKKLEEDIAKLYFYQMLKAVE--------------YLHNNGIIHRDLKPENVLLASHDDICLIKITDFNQSKILEESSLMKTLCGTPTYVAPEVFTHASTVGYTKAVDYWSLGVLLFICLGGYPPFNTECTTMSVREQIINGEYRFIPSQWKNVSNEAKDLVKKLLVVDPQKRLSVEEALEHPWLKDDRMRQNANQLMNPGAANQ--------PMRPEA--TRKRKAQEGEGEPSSKRK 500
BLAST of Serine/threonine kinase vs. Ensembl Zebrafish
Match: camk1db (calcium/calmodulin-dependent protein kinase 1Db [Source:ZFIN;Acc:ZDB-GENE-070112-1872]) HSP 1 Score: 196.823 bits (499), Expect = 1.067e-57 Identity = 116/286 (40.56%), Postives = 160/286 (55.94%), Query Frame = 2
Query: 416 VRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGAT-MMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDD 1270
+R + LG GA EV LA TG+ A+K I KKAL K G+E E+ +LR++ H IV L D ++ L+++M+LV GGELFDR++ + E DA L+ Q++ D + YLH I HRDLKPEN+L + I ++DFGLSK A +M T CGTP Y+APEV+ + YS A D WS+GVI +I L GYPPF +E D L +QI + +Y F +W +S A I ++Q DP+KR T + L+HPW+ D
Sbjct: 20 IRKIFEFKEILGTGAFSEVVLAQEKATGDMYAVKCIPKKAL---RGKESGIENEIAVLRKIKHENIVALEDIYESPSHLYLIMQLVSGGELFDRIVERGFYTEQDASALIKQVL--------------DAVNYLHSLGIVHRDLKPENLLYFNPHEESKIMISDFGLSKMEGAANDIMSTACGTPGYVAPEVL---AQKPYSKAVDCWSIGVIAYILLCGYPPFYDE-NDSKLFEQILKAEYEFDSPYWDDISDSAKDFINNLMQKDPEKRFTCDEALRHPWIAGD 284
BLAST of Serine/threonine kinase vs. Ensembl Zebrafish
Match: camk1db (calcium/calmodulin-dependent protein kinase 1Db [Source:ZFIN;Acc:ZDB-GENE-070112-1872]) HSP 1 Score: 196.438 bits (498), Expect = 2.543e-57 Identity = 116/286 (40.56%), Postives = 161/286 (56.29%), Query Frame = 2
Query: 416 VRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGAT-MMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDD 1270
+R + LG GA EV LA TG+ A+K I KKAL+ K G+E E+ +LR++ H IV L D ++ L+++M+LV GGELFDR++ + E DA L+ Q++ D + YLH I HRDLKPEN+L + I ++DFGLSK A +M T CGTP Y+APEV+ + YS A D WS+GVI +I L GYPPF +E D L +QI + +Y F +W +S A I ++Q DP+KR T + L+HPW+ D
Sbjct: 20 IRKIFEFKEILGTGAFSEVVLAQEKATGDMYAVKCIPKKALR---GKESGIENEIAVLRKIKHENIVALEDIYESPSHLYLIMQLVSGGELFDRIVERGFYTEQDASALIKQVL--------------DAVNYLHSLGIVHRDLKPENLLYFNPHEESKIMISDFGLSKMEGAANDIMSTACGTPGYVAPEVL---AQKPYSKAVDCWSIGVIAYILLCGYPPFYDE-NDSKLFEQILKAEYEFDSPYWDDISDSAKDFINNLMQKDPEKRFTCDEALRHPWIAGD 284
BLAST of Serine/threonine kinase vs. Ensembl Zebrafish
Match: camk4 (calcium/calmodulin-dependent protein kinase IV [Source:ZFIN;Acc:ZDB-GENE-050417-76]) HSP 1 Score: 194.897 bits (494), Expect = 5.424e-57 Identity = 112/288 (38.89%), Postives = 161/288 (55.90%), Query Frame = 2
Query: 428 YTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAM 1291
Y + LG+GA V GT + A+K++ K + + V E+ +L +L+HP I+KL++ +T + +V+ELV GGELFDRV+ + E DA + Q++ A+ YLH+N + HRDLKPEN+L AT +K+ DFGLSK VD MKT+CGTP Y APE++ G Y D+WS+GVI +I L G+ PF ++R D + K+I +Y F W VS A L+K+++ DPKKR+T + LQHPW+ V T M
Sbjct: 29 YELESELGRGATSVVYRCRQKGTQKHYAVKMLKK------TVDKKIVRTEIGVLLRLSHPNIIKLKEIFETPAEISLVLELVTGGELFDRVVEKGYYSERDAADAVKQVLEAVA--------------YLHENGVVHRDLKPENLLYATSAPDAPLKIADFGLSKIVDDQVTMKTVCGTPGYCAPEILRGCA---YGPEVDMWSVGVITYILLCGFEPFFDDRGDQYMFKRILNCEYEFVSPWWDNVSLNAKDLVKKLIVQDPKKRLTTQQALQHPWVTGKAVNFTHM 293
BLAST of Serine/threonine kinase vs. Ensembl Xenopus
Match: gfral (GDNF family receptor alpha like [Source:Xenbase;Acc:XB-GENE-6002264]) HSP 1 Score: 320.857 bits (821), Expect = 1.070e-103 Identity = 174/421 (41.33%), Postives = 258/421 (61.28%), Query Frame = 2
Query: 83 WGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSLCPY------FSSLSKIHFEIFKE-----NQNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLESTQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQ---GVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMY 1303
WG + L F + Y GR K P + + SK HF IF+E + + DLS NGT+VN +G+ K L +N I+L+ NK Q +YP +KY +SR +G GACGEVKLA+ +KVA+KII K+ K++++ ++ V+ E+EIL++++HPCI+K+ + D+++ +IV+EL+EGGELFD+V+S + E A++ YQM++A++ YLH+N + HRDLKPEN+LL++ IK+TDFG SK + ++M+TLCGTP YLAPEV+ GT YS A D WSLGVILF+CL+GYPPF+E+ + +PL QI EG+Y++ W VS A+ L+K++L VDP++R+T + L+HPW+ DD +K TA ++MY
Sbjct: 64 WGRLWALGRGFLNHDCLLEEYVFGRDKKCDYTFDIPVINQTERYKTYSKRHFRIFREFGHGHSPVAYIEDLSGNGTFVNKEIIGRGKKLPLTNNAEIALSLSSNKDLSV----DDQTIYPKDFSDKYIMSRPIGSGACGEVKLAFQKSACKKVAVKIISKRKFKMSTSSNEHPISVDTEIEILKKIDHPCIIKIENFFDSEDFFYIVLELMEGGELFDKVVSSTRLRESTAKLYFYQMLLAVQ--------------YLHENGVIHRDLKPENVLLSSSNEECCIKITDFGQSKILGETSLMRTLCGTPTYLAPEVLNTAGTTGYSRAVDYWSLGVILFVCLSGYPPFSEQNSKIPLKNQIAEGKYTYIPAAWDAVSEQALDLVKKLLVVDPEQRLTTKQALEHPWLQDDDMKHTAERLMY 466
BLAST of Serine/threonine kinase vs. Ensembl Xenopus
Match: gfral (GDNF family receptor alpha like [Source:Xenbase;Acc:XB-GENE-6002264]) HSP 1 Score: 309.686 bits (792), Expect = 3.494e-99 Identity = 171/422 (40.52%), Postives = 242/422 (57.35%), Query Frame = 2
Query: 83 WGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSLCPY------FSSLSKIHFEIFKE-----NQNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLE-STQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLN---HPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMY 1303
WG + L F + Y GR K P + + SK HF IF+E + + DLS NGT+VN +G+ K L +N I+L+ NK FVF L Q +YP +KY +SR +G GACGEVKLA+ + K I K ST S + L PCI+K+ + D+++ +IV+EL+EGGELFD+V+S + E A++ YQM++A++ YLH+N + HRDLKPEN+LL++ IK+TDFG SK + ++M+TLCGTP YLAPEV+ GT YS A D WSLGVILF+CL+GYPPF+E+ + +PL QI EG+Y++ W VS A+ L+K++L VDP++R+T + L+HPW+ DD +K TA ++MY
Sbjct: 64 WGRLWALGRGFLNHDCLLEEYVFGRDKKCDYTFDIPVINQTERYKTYSKRHFRIFREFGHGHSPVAYIEDLSGNGTFVNKEIIGRGKKLPLTNNAEIALSLSSNKVFVFSDLSVDDQTIYPKDFSDKYIMSRPIGSGACGEVKLAF-QKSACKKVAVKIISKRKFKMSTSSNVTPFAAVVTYHLFFCFQPCIIKIENFFDSEDFFYIVLELMEGGELFDKVVSSTRLRESTAKLYFYQMLLAVQ--------------YLHENGVIHRDLKPENVLLSSSNEECCIKITDFGQSKILGETSLMRTLCGTPTYLAPEVLNTAGTTGYSRAVDYWSLGVILFVCLSGYPPFSEQNSKIPLKNQIAEGKYTYIPAAWDAVSEQALDLVKKLLVVDPEQRLTTKQALEHPWLQDDDMKHTAERLMY 470
BLAST of Serine/threonine kinase vs. Ensembl Xenopus
Match: gfral (GDNF family receptor alpha like [Source:Xenbase;Acc:XB-GENE-6002264]) HSP 1 Score: 299.286 bits (765), Expect = 1.168e-95 Identity = 171/408 (41.91%), Postives = 243/408 (59.56%), Query Frame = 2
Query: 83 WGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSLCPY------FSSLSKIHFEIFKE-----NQNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLE-STQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDD 1270
WG + L F + Y GR K P + + SK HF IF+E + + DLS NGT+VN +G+ K L +N I+L+ NK FVF L Q +YP +KY +SR +G GACGEVKLA+ +KVA+KII K+ K+++ S V + Q PCI+K+ + D+++ +IV+EL+EGGELFD+V+S + E A++ YQM++A++ YLH+N + HRDLKPEN+LL++ IK+TDFG SK + ++M+TLCGTP YLAPEV+ GT YS A D WSLGVILF+CL+GYPPF+E+ + +PL QI EG+Y++ W VS A+ L+K++L VDP++R+T + L+HPWMN D
Sbjct: 64 WGRLWALGRGFLNHDCLLEEYVFGRDKKCDYTFDIPVINQTERYKTYSKRHFRIFREFGHGHSPVAYIEDLSGNGTFVNKEIIGRGKKLPLTNNAEIALSLSSNKVFVFSDLSVDDQTIYPKDFSDKYIMSRPIGSGACGEVKLAFQKSACKKVAVKIISKRKFKMST--STVVTYHLFFCFQ---PCIIKIENFFDSEDFFYIVLELMEGGELFDKVVSSTRLRESTAKLYFYQMLLAVQ--------------YLHENGVIHRDLKPENVLLSSSNEECCIKITDFGQSKILGETSLMRTLCGTPTYLAPEVLNTAGTTGYSRAVDYWSLGVILFVCLSGYPPFSEQNSKIPLKNQIAEGKYTYIPAAWDAVSEQALDLVKKLLVVDPEQRLTTKQALEHPWMNSD 452
BLAST of Serine/threonine kinase vs. Ensembl Xenopus
Match: ENSXETT00000024431.1 (serine/threonine-protein kinase Chk2-like [Source:NCBI gene;Acc:105946289]) HSP 1 Score: 233.417 bits (594), Expect = 3.485e-72 Identity = 107/234 (45.73%), Postives = 156/234 (66.67%), Query Frame = 2
Query: 602 HPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMY 1303
PCI+K+ + D+++ +IV+EL+EGGELFD+V+S + E A++ YQM++A++ YLH+N + HRDLKPEN+LL++ IK+TDFG +TLCGTP Y+APEV+ GT YS A D WSLGVILF+CL+GYPPF+E+ + +PL QI EG+Y++ W VS A+ L+K++L VDP++R+T + L+HPW+ DD +K TA ++MY
Sbjct: 54 QPCIIKIENFFDSEDFFYIVLELMEGGELFDKVVSSTRLRESTAKLYFYQMLLAVQ--------------YLHENGVIHRDLKPENVLLSSSNEECCIKITDFG-----------QTLCGTPTYVAPEVLNTAGTTGYSRAVDYWSLGVILFVCLSGYPPFSEQNSKIPLKNQIAEGKYTYIPAAWDAVSEQALDLVKKLLVVDPEQRLTTKQALEHPWLQDDDMKHTAERLMY 262
BLAST of Serine/threonine kinase vs. Ensembl Xenopus
Match: gfral (GDNF family receptor alpha like [Source:Xenbase;Acc:XB-GENE-6002264]) HSP 1 Score: 217.238 bits (552), Expect = 5.719e-65 Identity = 133/341 (39.00%), Postives = 183/341 (53.67%), Query Frame = 2
Query: 83 WGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSLCPY------FSSLSKIHFEIFKE-----NQNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLE-STQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLN---HPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFI 1060
WG + L F + Y GR K P + + SK HF IF+E + + DLS NGT+VN +G+ K L +N I+L+ NK FVF L Q +YP +KY +SR +G GACGEVKLA+ + K I K ST S + L PCI+K+ + D+++ +IV+EL+EGGELFD+V+S + E A++ YQM++A++ YLH+N + HRDLKPEN+LL++ IK+TDFG SK + ++M+TLCGTP YLAPEV+ GT YS A D WSLGVILF+
Sbjct: 64 WGRLWALGRGFLNHDCLLEEYVFGRDKKCDYTFDIPVINQTERYKTYSKRHFRIFREFGHGHSPVAYIEDLSGNGTFVNKEIIGRGKKLPLTNNAEIALSLSSNKVFVFSDLSVDDQTIYPKDFSDKYIMSRPIGSGACGEVKLAF-QKSACKKVAVKIISKRKFKMSTSSNVTPFAAVVTYHLFFCFQPCIIKIENFFDSEDFFYIVLELMEGGELFDKVVSSTRLRESTAKLYFYQMLLAVQ--------------YLHENGVIHRDLKPENVLLSSSNEECCIKITDFGQSKILGETSLMRTLCGTPTYLAPEVLNTAGTTGYSRAVDYWSLGVILFV 389
BLAST of Serine/threonine kinase vs. Ensembl Mouse
Match: Chek2 (checkpoint kinase 2 [Source:MGI Symbol;Acc:MGI:1355321]) HSP 1 Score: 337.421 bits (864), Expect = 1.626e-109 Identity = 197/443 (44.47%), Postives = 275/443 (62.08%), Query Frame = 2
Query: 29 NTQELFS---QNDAEIINSNAWGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSLCPY------------FSSLSKIHFEIFKE--NQN---VILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLE-STQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTK----SQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSF-PEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVK 1279
+TQEL S + E W + L F ++ + +Y GR + C Y + + SK HF IF+E +N V + D S NGT+VN +GK K L +N I+L+ +NK FVF L Q +YP +R++Y +S+TLG GACGEVK+A+ T +KVAIKII K+ L S++ + VE E+EIL++LNHPCI+K++D D E +IV+EL+EGGELFDRV+ K+ +E ++ YQM++A++ YLH+N I HRDLKPEN+LL++ + LIK+TDFG SK + ++M+TLCGTP YLAPEV++ NGT YS A D WSLGVILFICL+GYPPF+E +T + L QIT G+Y+F PEV W+ VS A+ L+K++L VDPK R+T + L HPW+ D+ +K
Sbjct: 76 STQELCSIPEDQEPEEPGPAPWARLWALQDGFSNLDCVNDNYWFGR------DKSCEYCFDGPLLRRTDKYRTYSKKHFRIFREMGPKNCYIVYIEDHSGNGTFVNTELIGKGKRCPLSNNSEIALSLCRNKVFVFFDLTVDDQSVYPKELRDEYIMSKTLGSGACGEVKMAFERKTCQKVAIKIISKRRFALGSSREADTAPSVETEIEILKKLNHPCIIKIKDVFDA-EDYYIVLELMEGGELFDRVVGNKRLKEATCKLYFYQMLVAVQ--------------YLHENGIIHRDLKPENVLLSSQEEDCLIKITDFGQSKILGETSLMRTLCGTPTYLAPEVLVSNGTAGYSRAVDCWSLGVILFICLSGYPPFSEHKTQVSLKDQITSGKYNFIPEV-WTDVSEEALDLVKKLLVVDPKARLTTEEALNHPWLQDEYMK 496
BLAST of Serine/threonine kinase vs. Ensembl Mouse
Match: Camk1 (calcium/calmodulin-dependent protein kinase I [Source:MGI Symbol;Acc:MGI:1098535]) HSP 1 Score: 199.134 bits (505), Expect = 2.015e-58 Identity = 112/285 (39.30%), Postives = 160/285 (56.14%), Query Frame = 2
Query: 416 VRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDD 1270
+R+ Y LG GA EV LA T + I A K K +E E+ +L ++ HP IV L D ++ L+++M+LV GGELFDR++ + E DA L++Q++ A+K YLHD I HRDLKPEN+L + + I ++DFGLSK D +++ T CGTP Y+APEV+ + YS A D WS+GVI +I L GYPPF +E D L +QI + +Y F +W +S A I+ +++ DP+KR T + LQHPW+ D
Sbjct: 16 IRDIYDFRDVLGTGAFSEVILAEDKRT---QKLVAIKCIAKKALEGKEGSMENEIAVLHKIKHPNIVALDDIYESGGHLYLIMQLVSGGELFDRIVEKGFYTERDASRLIFQVLDAVK--------------YLHDLGIVHRDLKPENLLYYSLDEDSKIMISDFGLSKMEDPGSVLSTACGTPGYVAPEVL---AQKPYSKAVDCWSIGVIAYILLCGYPPFYDE-NDAKLFEQILKAEYEFDSPYWDDISDSAKDFIRHLMEKDPEKRFTCEQALQHPWIAGD 279
BLAST of Serine/threonine kinase vs. Ensembl Mouse
Match: Dclk1 (doublecortin-like kinase 1 [Source:MGI Symbol;Acc:MGI:1330861]) HSP 1 Score: 196.823 bits (499), Expect = 1.744e-57 Identity = 117/290 (40.34%), Postives = 162/290 (55.86%), Query Frame = 2
Query: 407 PMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVT-LIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLP-LSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDD 1270
P + +Y + RT+G G VK T + A+KII K K ++ EV ILR++ HP IV L + +D L++VMELV+GG+LFD + S ++ E DA +LY + AIK YLH NI HRD+KPEN+L+ Q+ + +K+ DFGL+ VDG + T+CGTP Y+APE+I G Y DIW+ GVI +I L G+PPF D L QI GQ FP +W VS A LI ML V+ +R + +++L+HPW+NDD
Sbjct: 76 PATITERYKVGRTIGDGNFAVVKECIERSTAREYALKIIKKSKC---RGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTSKYTERDASGMLYNLASAIK--------------YLHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATIVDGP--LYTVCGTPTYVAPEIIAETG---YGLKVDIWAAGVITYILLCGFPPFRGSGDDQEVLFDQILMGQVDFPSPYWDNVSDSAKELINMMLLVNVDQRFSAVQVLEHPWVNDD 343
BLAST of Serine/threonine kinase vs. Ensembl Mouse
Match: Dclk1 (doublecortin-like kinase 1 [Source:MGI Symbol;Acc:MGI:1330861]) HSP 1 Score: 197.593 bits (501), Expect = 2.822e-57 Identity = 116/289 (40.14%), Postives = 161/289 (55.71%), Query Frame = 2
Query: 407 PMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTL-IKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDD 1270
P + +Y + RT+G G VK T + A+KII K K ++ EV ILR++ HP IV L + +D L++VMELV+GG+LFD + S ++ E DA +LY + AIK YLH NI HRD+KPEN+L+ Q+ + +K+ DFGL+ VDG + T+CGTP Y+APE+I G Y DIW+ GVI +I L G+PPF L QI GQ FP +W VS A LI ML V+ +R + +++L+HPW+NDD
Sbjct: 76 PATITERYKVGRTIGDGNFAVVKECIERSTAREYALKIIKKSKC---RGKEHMIQNEVSILRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTSKYTERDASGMLYNLASAIK--------------YLHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATIVDGP--LYTVCGTPTYVAPEIIAETG---YGLKVDIWAAGVITYILLCGFPPFRGGDDQEVLFDQILMGQVDFPSPYWDNVSDSAKELINMMLLVNVDQRFSAVQVLEHPWVNDD 342
BLAST of Serine/threonine kinase vs. Ensembl Mouse
Match: Camk1d (calcium/calmodulin-dependent protein kinase ID [Source:MGI Symbol;Acc:MGI:2442190]) HSP 1 Score: 195.282 bits (495), Expect = 6.413e-57 Identity = 113/285 (39.65%), Postives = 159/285 (55.79%), Query Frame = 2
Query: 416 VRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDD 1270
++ + TLG GA EV LA TG+ A+K I KKAL K +E E+ +LR++ H IV L D ++ L++VM+LV GGELFDR++ + E DA L+ Q++ D + YLH I HRDLKPEN+L + + I ++DFGLSK +M T CGTP Y+APEV+ + YS A D WS+GVI +I L GYPPF +E D L +QI + +Y F +W +S A I+ +++ DP KR T + +HPW+ D
Sbjct: 19 IKKIFEFKETLGTGAFSEVVLAEEKATGKLFAVKCIPKKAL---KGKESSIENEIAVLRKIKHENIVALEDIYESPNHLYLVMQLVSGGELFDRIVEKGFYTEKDASTLIRQVL--------------DAVYYLHRMGIVHRDLKPENLLYYSQDEESKIMISDFGLSKMEGKGDVMSTACGTPGYVAPEVL---AQKPYSKAVDCWSIGVIAYILLCGYPPFYDE-NDSKLFEQILKAEYEFDSPYWDDISDSAKDFIRNLMEKDPNKRYTCEQAARHPWIAGD 282
BLAST of Serine/threonine kinase vs. UniProt/SwissProt
Match: sp|Q9Z265|CHK2_MOUSE (Serine/threonine-protein kinase Chk2 OS=Mus musculus OX=10090 GN=Chek2 PE=1 SV=1) HSP 1 Score: 337.421 bits (864), Expect = 1.137e-108 Identity = 197/443 (44.47%), Postives = 275/443 (62.08%), Query Frame = 2
Query: 29 NTQELFS---QNDAEIINSNAWGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSLCPY------------FSSLSKIHFEIFKE--NQN---VILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLE-STQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTK----SQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSF-PEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVK 1279
+TQEL S + E W + L F ++ + +Y GR + C Y + + SK HF IF+E +N V + D S NGT+VN +GK K L +N I+L+ +NK FVF L Q +YP +R++Y +S+TLG GACGEVK+A+ T +KVAIKII K+ L S++ + VE E+EIL++LNHPCI+K++D D E +IV+EL+EGGELFDRV+ K+ +E ++ YQM++A++ YLH+N I HRDLKPEN+LL++ + LIK+TDFG SK + ++M+TLCGTP YLAPEV++ NGT YS A D WSLGVILFICL+GYPPF+E +T + L QIT G+Y+F PEV W+ VS A+ L+K++L VDPK R+T + L HPW+ D+ +K
Sbjct: 76 STQELCSIPEDQEPEEPGPAPWARLWALQDGFSNLDCVNDNYWFGR------DKSCEYCFDGPLLRRTDKYRTYSKKHFRIFREMGPKNCYIVYIEDHSGNGTFVNTELIGKGKRCPLSNNSEIALSLCRNKVFVFFDLTVDDQSVYPKELRDEYIMSKTLGSGACGEVKMAFERKTCQKVAIKIISKRRFALGSSREADTAPSVETEIEILKKLNHPCIIKIKDVFDA-EDYYIVLELMEGGELFDRVVGNKRLKEATCKLYFYQMLVAVQ--------------YLHENGIIHRDLKPENVLLSSQEEDCLIKITDFGQSKILGETSLMRTLCGTPTYLAPEVLVSNGTAGYSRAVDCWSLGVILFICLSGYPPFSEHKTQVSLKDQITSGKYNFIPEV-WTDVSEEALDLVKKLLVVDPKARLTTEEALNHPWLQDEYMK 496
BLAST of Serine/threonine kinase vs. UniProt/SwissProt
Match: sp|O96017|CHK2_HUMAN (Serine/threonine-protein kinase Chk2 OS=Homo sapiens OX=9606 GN=CHEK2 PE=1 SV=1) HSP 1 Score: 335.88 bits (860), Expect = 3.477e-108 Identity = 192/440 (43.64%), Postives = 269/440 (61.14%), Query Frame = 2
Query: 83 WGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSLCPY------------FSSLSKIHFEIFKE-----NQNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLE-STQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQ----GVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSF-PEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYPLSEVTKLCQ 1333
W + L F +E + +Y GR + C Y + + SK HF IF+E + + D S NGT+VN VGK K L +N I+L+ +NK FVF L Q +YP +R++Y +S+TLG GACGEVKLA+ T +KVAIKII K+ + S + VE E+EIL++LNHPCI+K+++ D E +IV+EL+EGGELFD+V+ K+ +E ++ YQM++A++ YLH+N I HRDLKPEN+LL++ + LIK+TDFG SK + ++M+TLCGTP YLAPEV++ GT Y+ A D WSLGVILFICL+GYPPF+E RT + L QIT G+Y+F PEV W+ VS A+ L+K++L VDPK R T + L+HPW+ D+ +K ++ +E T L Q
Sbjct: 93 WARLWALQDGFANLECVNDNYWFGR------DKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSVYPKALRDEYIMSKTLGSGACGEVKLAFERKTCKKVAIKIISKRKFAIGSAREADPALNVETEIEILKKLNHPCIIKIKNFFDA-EDYYIVLELMEGGELFDKVVGNKRLKEATCKLYFYQMLLAVQ--------------YLHENGIIHRDLKPENVLLSSQEEDCLIKITDFGHSKILGETSLMRTLCGTPTYLAPEVLVSVGTAGYNRAVDCWSLGVILFICLSGYPPFSEHRTQVSLKDQITSGKYNFIPEV-WAEVSEKALDLVKKLLVVDPKARFTTEEALRHPWLQDEDMKRKFQDLLSEENESTALPQ 510
BLAST of Serine/threonine kinase vs. UniProt/SwissProt
Match: sp|O61267|LOK_DROME (Ovarian-specific serine/threonine-protein kinase Lok OS=Drosophila melanogaster OX=7227 GN=lok PE=2 SV=1) HSP 1 Score: 283.108 bits (723), Expect = 1.356e-88 Identity = 163/461 (35.36%), Postives = 261/461 (56.62%), Query Frame = 2
Query: 14 EDSQFNTQELFSQNDAEIINSNAWGAFFGLLGKFPC-----------------VELTDSSYTVGRGGK----IILNSLC-PYFSSLSKIHFEIFKEN----QNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLESTQHL-YPMVVRNKYTISRTLGKGACGEVKLAYVNGTGE--XXXXXXXXXXXXXXXSTKSQGVER---EVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIM 1300
+ +Q +++Q +++ + WG +G K V+L + +T GRG + LN L + +SK+HF I + N V ++DLS+NGT+VN+ K+G ++ +L+++DVISL+ KAFVF L + + P + Y ++R LG GA G V+L Y T + + I + + ST +R E +I++ L+HPC+V++ D +D + +++V+E + GG+L +R+IS K E +++ YQM A+K YLHD I+HRDLKP+N+LL T+ TL+KV+DFGLSKFV ++M+TLCGTP Y+APEV++ G + Y+ DIWSLGV+LF CL+G PF++E P ++QI +G++++ W VS+ A LI QML VDP++R ++ +LQ W+ D + A ++M
Sbjct: 9 QGTQSQASNIWTQVESQPMEKIVWGRLYGKNIKIKSLGTSSKYRIIYTHSSFSVDLNNDEFTAGRGEANDLILTLNDLPEKILTRISKVHFIIKRANCELTNPVYIQDLSRNGTFVNNEKIGTNRMRILKNDDVISLSHPTYKAFVFKDLSPNESIGLPEEINKTYYVNRKLGSGAYGLVRLVYDTRTCQQFAMKIVKKNMLSGARPSTNFSDPDRVLNEAKIMKNLSHPCVVRMHDIVDKPDSVYMVLEFMRGGDLLNRIISNKLLSEDISKLYFYQMCHAVK--------------YLHDRGITHRDLKPDNVLLETNDEETLLKVSDFGLSKFVQKDSVMRTLCGTPLYVAPEVLITGGREAYTKKVDIWSLGVVLFTCLSGTLPFSDE-YGTPAAQQIKKGRFAYGHPSWKSVSQRAKLLINQMLIVDPERRPSIDDVLQSSWLRDAPMLQKAKRLM 454
BLAST of Serine/threonine kinase vs. UniProt/SwissProt
Match: sp|P34101|FHKC_DICDI (Probable serine/threonine-protein kinase fhkC OS=Dictyostelium discoideum OX=44689 GN=fhkC PE=3 SV=2) HSP 1 Score: 240.736 bits (613), Expect = 4.364e-71 Identity = 148/412 (35.92%), Postives = 237/412 (57.52%), Query Frame = 2
Query: 83 WGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSLCPY-FSS--LSKIHFEIFKE----NQNV-ILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNK---AFVFMH-LESTQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKT 1282
WG L +P +E+ S +GR +C Y F+S +S H +I+++ ++NV + D S NGT++N+ +GK L+++ IS+ +K +F++ E + + + KY + LG G V+L TG K AIKIIDKK + + S + + EV +L ++ H I+ +++ +T + L++V+ELV GGELFD+++S ++F+E + +L Q+ D++ YLH N I+HRDLKPENILLAT N L+K++DFGLS+ +D T MKT+CGTP Y+APE++ + Y + D+WS+GVI +I L G+PPF + +T ++I G +SFP +W +S A LIK +++VD +KR T+ + L HPW + + KT
Sbjct: 96 WGKLISLNPTYPTIEIRQDSIILGRS-----KGVCNYTFTSPTVSGKHCKIYRDPTVKSRNVAFVDDTSTNGTFINNEVIGKGSKILIENGCEISVIPKKGSEKISFIYQDCFEEQKEMEQGGPQQKYDLREVLGTGNFASVRLGVEKETGNKYAIKIIDKKKMSMTSKRKDSLMDEVNVLTKVKHQNIISIKEVFETQKNLYLVLELVTGGELFDKIVSERKFQEDTCRYILKQLC--------------DSVRYLHSNGIAHRDLKPENILLAT-PNSFLLKISDFGLSRAMDEGTYMKTMCGTPQYVAPEILTKGEREGYGKSVDLWSIGVITYILLCGFPPFGDPQTK-DFFEKIKNGGFSFPSPYWDEISDEAKSLIKNLIKVDVEKRFTIDQALNHPWFTNHEEKT 486
BLAST of Serine/threonine kinase vs. UniProt/SwissProt
Match: sp|Q54VI1|FHKE_DICDI (Probable serine/threonine-protein kinase fhkE OS=Dictyostelium discoideum OX=44689 GN=fhkE PE=3 SV=1) HSP 1 Score: 234.187 bits (596), Expect = 1.138e-67 Identity = 153/410 (37.32%), Postives = 221/410 (53.90%), Query Frame = 2
Query: 80 AWGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSLCPYFS-SLSKIHFEIFKENQN------VILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNK---AFVFMHLESTQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTG-EXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLAT---DQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQE-YSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMN 1264
WG L +P + L ++ T GR + +S Y S+S H +I +E+ + + D S NGT+++++KVGK LL S ISL QK A++F +E + + NKY I LG+G VKLA TG + + KK +S + + EV ILR L+HP I+++ + + ++ L +++ELVE GEL + ++S + E A+ L Q+V D + YLH+ I+HRDLKPENILL +QN IK+TDFGLS+ V + MKT+CGTP YLAPE++ +G Y D WS+G IL+I L GYPPF + R ++ + +QI ++ F WS VS A LIK++L VDP KR T I+QHPW N
Sbjct: 25 GWGILKSLNPSYPDISLVENVITFGR----LKDSTVHYNDKSISGSHCKITRESNDDDGVVIAFIYDNSTNGTFIDNIKVGKGSRCLLASGQEISLTPQKQLEKIAYIFQTVE--KEIEDGGPSNKYFIGEMLGQGNFATVKLAVERTTGVKYAVKIVDKKKYFMNSSARKDSLMDEVNILRGLSHPNIIQIIEVFENEKVLSLILELVECGELLNDIVSNLFYTEDKAKTLFRQIV--------------DGVLYLHNKGIAHRDLKPENILLKHKNFNQN-DAIKLTDFGLSRTVSDGSFMKTMCGTPQYLAPEILTSSGGHNGYGLEVDCWSMGAILYIMLCGYPPFDDSR-EVSIFEQIRNAKFEFDPEDWSSVSEEAKDLIKRLLCVDPHKRYTCNNIIQHPWFN 412
BLAST of Serine/threonine kinase vs. TrEMBL
Match: A0A0B6XZ20 (Uncharacterized protein (Fragment) OS=Arion vulgaris OX=1028688 GN=ORF6441 PE=4 SV=1) HSP 1 Score: 422.55 bits (1085), Expect = 6.811e-140 Identity = 224/437 (51.26%), Postives = 290/437 (66.36%), Query Frame = 2
Query: 53 NDAEIINSNAWGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSLCPY-------FSSLSKIHFEIFKENQN----VILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLESTQHL-YPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTK----SQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYPLSE 1315
+D E AWG F L F ++L YT GRG + + C F + SK+HF++ ++N N + L DLS NGT++N KVGK K H+L +ND ISLA +KNKA+VFM L + ++ P + KYT++R LG+GACGEV+LA+ G+ +K A K+I KK + + V EV+IL+ L HPCI+ + D IDT + L+I++ELVEGGELFD+VISI +++EV A++L +QM AIK YLHD I+HRDLKPENILL++D TLIKVTDFGLSKFVD +MMKT CGTP YLAPE+IL G+ Y+ A D WSLGVILFI L+GYPPF++ER D+ L KQIT G +SFP+ +W+ VS A+ LIKQM+ VDPKKRIT IL HPWM DD+VKT A +MYP SE
Sbjct: 56 SDEESDQPEAWGRLFPLGSSFVAIDLISDEYTFGRGEECDV-PFCSQAAKIHQCFQAYSKVHFKLIRKNTNTGTHIFLEDLSSNGTFINGEKVGKGKKHVLNNNDEISLAVKKNKAYVFMDLNAHENGNLPQELTEKYTLTRVLGRGACGEVRLAFAKGSCDKYACKLISKKKFSVGGKTHVNLASQVMGEVKILKALKHPCIIGIEDVIDTPDMLYIILELVEGGELFDKVISIDKYDEVTAKLLFFQMATAIK--------------YLHDQGITHRDLKPENILLSSDDMETLIKVTDFGLSKFVDAGSMMKTFCGTPTYLAPEIILTAGSGAYTKAIDCWSLGVILFIMLSGYPPFSDERKDMDLPKQITGGHFSFPKQYWTDVSEEAVDLIKQMMVVDPKKRITFADILNHPWMKDDEVKTRANALMYPNSE 477
BLAST of Serine/threonine kinase vs. TrEMBL
Match: A0A2C9JI89 (Uncharacterized protein OS=Biomphalaria glabrata OX=6526 GN=106067930 PE=4 SV=1) HSP 1 Score: 415.231 bits (1066), Expect = 1.314e-137 Identity = 225/440 (51.14%), Postives = 293/440 (66.59%), Query Frame = 2
Query: 44 FSQNDAEIINSNAWGAFFGLLGKFPCVELTDSSYTVGRGGKIIL---NSLCPY---FSSLSKIHFEIFKENQN----VILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLESTQ-HLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQ-----GVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYPLSE 1315
FS+ D ++ SNAWG F L F ++LT SY+ GRG + NS F + SKIHF++ ++ N V L D S NGT++N KVGK+K +L +ND I+LA +KNKA+VFM L + + P + KYT++R LGKGACGEV+LA+ G+ EK A KII KK + KSQ V EV+IL+ L HPCI+K+ D IDT + L+I++ELVEGGELFD+V+SI +++E ++L YQM AIK YLHD I+HRDLKPENILLA+D TLIKVTDFGLSKFVD +MMKT CGTP YLAPE++ GT Y+ A D WSLGVILFI L+GYPPF++ERTD+ L KQI G +SFP+ +W VS AI LIK+M+ +DPKKRIT +IL HPW+ D+++K A +M+P S+
Sbjct: 14 FSEED--LLQSNAWGRLFPLGASFVAMDLTADSYSFGRGEDCDIPFSNSASRKHQCFQAYSKIHFKLHRQKTNTGIHVFLEDTSSNGTFINGEKVGKNKKQVLNNNDEIALAMKKNKAYVFMDLHTKEDSTLPSALTEKYTLTRVLGKGACGEVRLAFAKGSCEKFACKIISKKKFSVGG-KSQLNLASQVMGEVKILKSLKHPCIIKIEDVIDTPDTLYILLELVEGGELFDKVVSIDKYDEKTGKLLFYQMASAIK--------------YLHDEGITHRDLKPENILLASDDLETLIKVTDFGLSKFVDAGSMMKTFCGTPTYLAPEILKSAGTGAYTKAIDCWSLGVILFIMLSGYPPFSDERTDMELPKQIVGGHFSFPKQYWKDVSDKAIDLIKKMMVIDPKKRITFAEILNHPWLKDEEMKAKANALMFPNSD 436
BLAST of Serine/threonine kinase vs. TrEMBL
Match: A0A210R6F6 (Serine/threonine-protein kinase Chk2 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT16245 PE=4 SV=1) HSP 1 Score: 414.461 bits (1064), Expect = 1.182e-136 Identity = 219/463 (47.30%), Postives = 298/463 (64.36%), Query Frame = 2
Query: 83 WGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSLCP------YFSSLSKIHFEIFKENQ----NVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLESTQHL-YPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTK----SQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYPLSEVTKLCQEDLGPCSPSAVGTVNENDNSLSG-EPVVKRR 1423
WG F L F +EL+ YT GRG + P YF + SK+HF++ ++ ++ L D S NGT++N KVGK +L +ND +SLA +KNKA++F+ + + P +R KYT+++TLG+GACGEVKLA+ G+ E+ A+KII KK + S+ V EV+IL+ L HPCI+ + D IDT + LFI++E+VEGGELFD+V+SI Q++E A++L YQMVMA K YLHD I+HRDLKPENILLA++ N T+IKVTDFGLSKFVD ++MKT CGTP YLAPE++L G+ Y+ + D WSLGVILFICLAGYPPF++ER D+ L KQI +SFP+ +WSG+S A+ LIK+++ VDPKKRITL L HPW D+++K A K++YP E G PS + + + + SG EP KR+
Sbjct: 64 WGKLFPLGQSFTAIELSKDEYTFGRGDNCDVPFCAPSAKKHQYFQAYSKVHFKLIRQKTSTGVHIFLEDTSGNGTFINGEKVGKGNKQVLANNDEVSLAVKKNKAYMFLDNNANEDTSLPQEIREKYTLTKTLGRGACGEVKLAFAKGSCERFAVKIISKKKFSIGGNSQINLSKQVMSEVKILKALKHPCIIAIEDVIDTPDTLFIILEVVEGGELFDKVVSIGQYDEPTAKLLFYQMVMACK--------------YLHDQGITHRDLKPENILLASENNETVIKVTDFGLSKFVDAGSIMKTFCGTPTYLAPEILLTAGSGAYTKSIDCWSLGVILFICLAGYPPFSDERKDMDLPKQILGAHFSFPKQYWSGISENAVDLIKKLMTVDPKKRITLADALNHPWFKDEEMKQKANKLIYPEHE---------GMAPPSILPSPPKKRVAESGEEPPAKRK 503
BLAST of Serine/threonine kinase vs. TrEMBL
Match: A0A2T7PDM7 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_06924 PE=4 SV=1) HSP 1 Score: 405.216 bits (1040), Expect = 1.747e-133 Identity = 225/488 (46.11%), Postives = 304/488 (62.30%), Query Frame = 2
Query: 2 LKSMEDSQFNTQELFSQNDAEIINSNAWGAFFGLLGKFPCVELTDSSYTVGRGGKIILN-----SLCPYFSSLSKIHFEIFKEN----QNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLESTQHL-YPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTK----SQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYPLSEVTKLCQEDLGPCSPSAVGTVNENDNSLSGEPVVKRR 1423
+++++ N FS +D AWG F L F V+L YT GRG ++ +F + SKIHF++ ++N +V L D S NGT+VN KVGK +L +ND I+LA +KNKA+VFM L S + + P ++ KYT+++ LG+GACGEV+LA+V GT +K+A KII KK + + V EV+IL+ L HPCI+ + D IDT + L+I++ELVEGGELFD+VISI+++ E A++L YQM A K YLHD I+HRDLKPENILLATD TLIKVTDFGLSKFVD +MMKT CGTP YLAPE+++ G+ Y+ A D WSLGV+LFI L+GYPPF++ER D+ L KQI G Y+FP +W +S AI LIK++L VDPKKRITL L HPW+ D+++K A K+M+P S+ + ++ PC G D+ P KR+
Sbjct: 1 METLQTQDVNDDYSFS-DDENHPQDEAWGRLFPLGESFIAVDLVKDDYTFGRGDDCDISFSSTGKRLQFFQACSKIHFKLIRKNTSTGMHVFLEDTSSNGTFVNGEKVGKGNRQVLGNNDEIALAMKKNKAYVFMDLTSGEDVDLPKELKEKYTLTKVLGRGACGEVRLAFVKGTCDKLACKIISKKKFSIGGNSQINMTSKVMEEVKILKSLKHPCIISIEDVIDTPDILYILLELVEGGELFDKVISIEKYPEPTAKLLFYQMACACK--------------YLHDQGITHRDLKPENILLATDSEETLIKVTDFGLSKFVDAGSMMKTFCGTPTYLAPEILVTAGSGAYTKAIDCWSLGVVLFILLSGYPPFSDERKDMDLPKQIMGGHYTFPAQYWKDISEDAIDLIKKLLTVDPKKRITLTDALGHPWLKDEEMKNRANKLMFPASD--GMAPPNIVPC-----GKKRTLDSVEDTSPEAKRK 466
BLAST of Serine/threonine kinase vs. TrEMBL
Match: K1R1E4 (Serine/threonine-protein kinase Chk2 OS=Crassostrea gigas OX=29159 GN=CGI_10023238 PE=4 SV=1) HSP 1 Score: 404.445 bits (1038), Expect = 5.252e-133 Identity = 215/440 (48.86%), Postives = 288/440 (65.45%), Query Frame = 2
Query: 44 FSQNDAEIINSNAWGAFFGLLGKFPCVELTDSSYTVGRGG--------KIILNSLCPYFSSLSKIHFEIFKENQN----VILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLESTQ-HLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTK----SQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYPLS 1312
FS + E + W L F V+L + YT GRG ++ N C F + SK+HF++ K+ + V L D S NGT+VN KVGK L +ND I+LA +KNKA++++ L + + P +R+KYT+ RTLGKGACGEVK+A+ GT E+ A+KII KK + Q V EV++L+ L HPCI+K+ D +D+ + L+IV+ELV+GGELFD+V+SI Q++E A+++ YQMV A+K YLHD I+HRDLKPENILLAT++N TLIKVTDFGLSKFVD TMMKT CGTP+YLAPE++L G Y+ A D WSLGVI++ICL GYPPF++ER D+ L+KQI G YSFP+ +W +S AI L+K++L VDPKKR TL +++ HPW DD +K A K+MYP S
Sbjct: 32 FSGEEEEAEETQPWARLMPLGPGFVTVDLKNDEYTFGRGENCDVSFSVQMAKNKQC--FQAYSKVHFKLIKQTTSTGEFVFLEDTSSNGTFVNGEKVGKGNKQALMNNDEIALALKKNKAYMYLDLNTDEDKTLPAEIRDKYTLGRTLGKGACGEVKVAFSKGTCERFAVKIISKKKFTIGGVNQVNLGQQVMNEVKVLKALKHPCIIKIEDVVDSPDVLYIVLELVDGGELFDKVVSIGQYDEPTAKLMSYQMVCAVK--------------YLHDEGITHRDLKPENILLATEENETLIKVTDFGLSKFVDAGTMMKTFCGTPSYLAPEILLTVGMGAYTKAIDCWSLGVIIYICLVGYPPFSDEREDMALNKQIIGGHYSFPKEYWKDISEEAIDLVKKLLTVDPKKRATLEEVIGHPWFKDDAMKKKANKLMYPES 455
BLAST of Serine/threonine kinase vs. Ensembl Cavefish
Match: chek2 (checkpoint kinase 2 [Source:NCBI gene;Acc:103034962]) HSP 1 Score: 341.658 bits (875), Expect = 1.353e-111 Identity = 181/415 (43.61%), Postives = 258/415 (62.17%), Query Frame = 2
Query: 83 WGAFFGLLGKFPCVELTDSSYTVGRGGK--------IILNSLCPYFSSLSKIHFEIFKENQNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHL-ESTQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIM 1300
WG L+ F + D+ Y GR + ILNS P + + SK HF+IF++ L+DLS NGT+V+D +VGK K L +N V+SLA K+K F+++ + Q +P R KY ++R +G G CGEV+LA T +KVA+K I+K T ++ ERE+EIL++++HPC+++ D T+E +IV+E VEGGELF ++ + KQ +E A++ YQM+ A++ YLH+N I HRDLKPEN+LL + ++ LIK+TDF SK ++ +++MKTLCGTP YLAPEV T Y+ A D WSLGV+LFICL GYPPF E + LP+ +QI GQY F W VS A L+K++L VDP KRI++ + LQHPW+ND +++ TA +M
Sbjct: 66 WGRMVPLIRGFNLLNCVDNVYCFGRDSRCDYAFKDPAILNS--PRYKTYSKRHFKIFRDGDLAYLQDLSGNGTWVDDTEVGKGKKIPLNNNSVVSLADPKHKVFMYVDMNHDEQPNFPKEFREKYHVTRKIGSGVCGEVRLAIERETCKKVALKTINKNDFPSVGTATRNAEREIEILKKIDHPCLIRTEDFYQTEESYYIVLEYVEGGELFTKIKTEKQLKEEIAKLYFYQMLKAVE--------------YLHENGIIHRDLKPENVLLTSHEDECLIKITDFNQSKILEESSLMKTLCGTPTYLAPEVFTAASTGGYTRAVDCWSLGVLLFICLGGYPPFNTESSPLPVREQIINGQYCFIRSQWKSVSNAAKDLVKKLLVVDPVKRISISEALQHPWLNDVEMRRTAHTLM 464
BLAST of Serine/threonine kinase vs. Ensembl Cavefish
Match: dclk1b (doublecortin-like kinase 1b [Source:ZFIN;Acc:ZDB-GENE-080516-7]) HSP 1 Score: 199.134 bits (505), Expect = 1.127e-55 Identity = 131/344 (38.08%), Postives = 189/344 (54.94%), Query Frame = 2
Query: 407 PMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVT-LIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDL-PLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYPLSEVTKLCQE-DLGP-CSPSAVGTVNENDNSLSGEPVVKRRK 1426
P + +Y + R +G G V+ TG + A+KII+K + K ++ EV ILR++ HP +V L + +DT L++VMELV+GG+LFD + S ++ E DA +LY + AIK YLH NI HRD+KPEN+L+ Q+ + +K+ DFGL+ VDG + T+CGTP Y+APE+I +G Y DIW+ GVI +I L G+PPF D L QI GQ FP +W VS A LI MLQV+ +R T +++L HPW+NDD + ++ + LS K+ + + GP S +A G +L E V RR+
Sbjct: 360 PASIAERYRVGRMIGDGNFAVVRECVERSTGREYALKIINKGKCRG---KEHMIQNEVSILRRVKHPNVVLLIEEMDTYSELYLVMELVKGGDLFDAITSTNKYTERDASGMLYNLASAIK--------------YLHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATVVDGP--LYTVCGTPTYVAPEIITESG---YGLKVDIWAAGVITYILLCGFPPFRGSTEDQEALFDQILMGQLDFPLPYWDNVSDSAKELITCMLQVEVNQRYTALQVLDHPWVNDDGM----LENEHQLSVAGKIKKHFNTGPKFSSTAAGVSVITTTALDKERQVLRRR 677
BLAST of Serine/threonine kinase vs. Ensembl Cavefish
Match: camk4 (calcium/calmodulin dependent protein kinase IV [Source:NCBI gene;Acc:103044753]) HSP 1 Score: 191.045 bits (484), Expect = 1.504e-55 Identity = 98/242 (40.50%), Postives = 142/242 (58.68%), Query Frame = 2
Query: 566 VEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAM 1291
V E+ +L +L+HP I+KL++ +T + +V+ELV GGELFDRV+ + E DA + Q++ A+ YLH++ + HRDLKPEN+L AT +K+ DFGLSK +D MKT+CGTP Y APE++ G Y D+WS+GVI +I L G+ PF ++R D + K+I +Y F W VS A L+K+++ DPKKR+T ++ LQHPW+ T M
Sbjct: 71 VRTEIGVLLRLSHPNIIKLKEIFETPLEISLVLELVTGGELFDRVVEKGYYSERDAADAIKQVLEAVA--------------YLHEHGVVHRDLKPENLLYATSAPDAPLKIADFGLSKIIDDQVTMKTVCGTPGYCAPEILRGCA---YGPEVDMWSVGVITYILLCGFEPFFDDRGDQYMFKRILNCEYEFVSPWWDNVSLNAKDLVKKLIVQDPKKRLTTLQALQHPWVTGKATNFTHM 295
BLAST of Serine/threonine kinase vs. Ensembl Cavefish
Match: dclk1a (doublecortin-like kinase 1a [Source:ZFIN;Acc:ZDB-GENE-061013-124]) HSP 1 Score: 197.978 bits (502), Expect = 3.421e-55 Identity = 120/297 (40.40%), Postives = 167/297 (56.23%), Query Frame = 2
Query: 386 ESTQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVT-LIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDL-PLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDD 1270
EST P + ++Y + R +G G V TG A+KII+K K Q ++ EV ILR++ HP +V L + +DT L++VMELV+GG+LFD + S ++ E DA +LY + AIK YLH NI HRD+KPEN+L+ Q+ + +K+ DFGL+ VDG + T+CGTP Y+APE+I G Y DIW+ GVI +I L G+PPF D L QI GQ FP +W VS A LI MLQV+ +R T +++L+HPW+ +D
Sbjct: 356 ESTAPAVPASISDRYRVGRMIGDGNFAVVHECVERSTGRAYALKIINKGKC---RGKEQMIQNEVAILRRVKHPNVVLLIEEMDTYSELYLVMELVKGGDLFDAITSATRYTERDASGMLYNLANAIK--------------YLHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATVVDGP--LYTVCGTPTYVAPEIIAETG---YGLKVDIWAAGVITYILLCGFPPFRGSTEDQEALFDQILLGQIEFPLPYWDNVSDSAKELITSMLQVEVDQRYTALQVLEHPWVTND 630
BLAST of Serine/threonine kinase vs. Ensembl Cavefish
Match: camk1gb (calcium/calmodulin-dependent protein kinase type 1D-like [Source:NCBI gene;Acc:103033882]) HSP 1 Score: 190.66 bits (483), Expect = 1.769e-54 Identity = 109/282 (38.65%), Postives = 156/282 (55.32%), Query Frame = 2
Query: 416 VRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWM 1261
+R + LG GA EV TG+ A+K + K ++ +E E+ +L+++ H IV L D +T ++VM+LV GGELFDR++ F E DA +++ Q++ + YLH NNI HRDLKPEN+L + + + I ++DFGLSK D +M T CGTP Y+APEV+ + YS A D WS+GVI +I L GYPPF EE T+ L +I + QY F W +S A I+ M+Q +PK R T + L+HPW+
Sbjct: 35 IREIFDFIEVLGSGASSEVFKVKEKSTGKFFAMKCVIK------KNRNTTLENEIAVLKRIKHNNIVALEDFYETQSHYYLVMQLVSGGELFDRLLDRGMFSEKDASMVIRQVLEGVH--------------YLHQNNIVHRDLKPENLLYYSQDDNSKIMISDFGLSKIEDNG-IMSTTCGTPGYVAPEVL---AQKPYSKAVDCWSIGVITYILLCGYPPFYEE-TETRLFSKIMKAQYEFDSPFWDNISESAKDFIRNMMQKNPKLRFTTDQALRHPWI 291
BLAST of Serine/threonine kinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003135.1 (pep scaffold:Pmarinus_7.0:GL491838:799:5626:1 gene:ENSPMAG00000002863.1 transcript:ENSPMAT00000003135.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 170.629 bits (431), Expect = 6.528e-50 Identity = 108/276 (39.13%), Postives = 153/276 (55.43%), Query Frame = 2
Query: 452 KGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELV----EGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYL---APEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPW 1258
+GA EV LA TG VAIK I K AL K VE EV +L ++ H I+ L D +TD L+++M+LV GGELFDR++ + E DA L+ Q++ A+ +LH + HRDLKPEN+L + ++ + I ++DFGLSK ++M T CGTP Y+ APEV+ + Y+ A D WS+GVI +I L GYPPF +E D L QI + +Y F +W +S A I+ +++ D +R T + LQHPW
Sbjct: 1 RGAFSEVVLAEERSTGRHVAIKCIAKTAL---RGKDNTVENEVCVLSRIKHENIIALEDIYETDSHLYLIMQLVLLLIRGGELFDRIVERGFYTERDASALIRQVLRAVS--------------HLHGQGVVHRDLKPENLLYYSAEDDSKIMISDFGLSKVEAPGSVMSTACGTPGYVACEAPEVL---AQKPYTRAVDCWSIGVISYILLCGYPPFYDE-VDSRLFDQILKAEYEFDSPYWDDISDSAKDFIRHLMEKDVAERFTCEQALQHPW 255
BLAST of Serine/threonine kinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008748.1 (pep scaffold:Pmarinus_7.0:GL476577:591:63029:-1 gene:ENSPMAG00000007907.1 transcript:ENSPMAT00000008748.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 172.17 bits (435), Expect = 2.634e-49 Identity = 106/281 (37.72%), Postives = 156/281 (55.52%), Query Frame = 2
Query: 422 NKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMK-TLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWM 1261
++Y + LGKGA V+ TG++ A KII+ K L + Q +ERE I R L H IV+L ++I + ++V +LV GGELF+ +++ + + E DA + Q++ +++ + H + I HRDLKPEN+LLA+ +K+ DFGL+ V G GTP YL+PEV+ + Y D+W+ GVIL+I L GYPPF +E L +QI G Y FP W V+ A +LI QML ++P KRIT + L+HPW+
Sbjct: 12 DEYQLYEELGKGAFSVVRRCVKVSTGQEFAAKIINTKKLS--ARDHQKLEREARICRLLKHSNIVRLHESIAEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQIL--------------ESVHHCHQHGIVHRDLKPENLLLASKMKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKD---PYGKPVDMWACGVILYILLVGYPPFWDEDQH-KLYQQIKAGAYDFPSPEWDTVTAEAKNLINQMLTINPAKRITAAEALKHPWI 272
BLAST of Serine/threonine kinase vs. Ensembl Sea Lamprey
Match: stk33 (serine/threonine kinase 33 [Source:ZFIN;Acc:ZDB-GENE-040724-242]) HSP 1 Score: 164.851 bits (416), Expect = 3.553e-47 Identity = 106/292 (36.30%), Postives = 162/292 (55.48%), Query Frame = 2
Query: 419 RNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQ-----NVTLIKVTDFGLSKFVDG---ATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDD 1270
+N Y + R LG G+ G V A TG V I + S+ + +EREV IL+++ H I+ L + ++T +R+++VMEL E GEL + K+ E +A+ + + + AI YLH ++I HRDLK ENIL + + + I+VTDFGLS +G A+M+++ CGTP Y+A EVI +Q+YS CD+WS+GVIL++ L G PPF + L + I +G+ SF + W VS A L++ +L+VDP R+T ++L W+ D
Sbjct: 2 QNYYRLGRQLGSGSFGTVYEATHVETG--VKWAIKTVNKERAGSSGVRLLEREVVILKRVQHQNIIHLEEVLETHKRVYLVMELCELGELRAVLEQRKRLSEAEARHVAHSLAGAIA--------------YLHKHDIVHRDLKLENILAKSSEPHEGIDHLDIRVTDFGLSAVREGNGAASMLQSPCGTPIYMAAEVI---QSQDYSQQCDVWSIGVILYMLLCGEPPFMAHSQE-KLFELIIKGELSFTQPAWEEVSESAKELLRGLLKVDPAHRLTASELLHDHWVTGD 273
BLAST of Serine/threonine kinase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006316.1 (pep scaffold:Pmarinus_7.0:GL479319:16843:32374:1 gene:ENSPMAG00000005682.1 transcript:ENSPMAT00000006316.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 159.073 bits (401), Expect = 7.667e-45 Identity = 89/243 (36.63%), Postives = 140/243 (57.61%), Query Frame = 2
Query: 566 VEREVEI-LRQLNHPCIVKLRDAID----TDERLFIVMELVEGGELFDRVISI--KQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEER---TDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMN 1264
REVE+ R P IV++ + + + + L IVME +EGGELF+R+ + F E +A ++ + AI+ +LH NI+HRD+KPEN+L + ++ ++K+TDFG +K + T C TP Y+APEV+ G ++Y +CD+WSLGVI++I L G+PPF + K+I GQY FP W+ VS A +I+++L+ DP +R+T+ + + HPW+N
Sbjct: 12 ARREVEMHWRASGCPHIVRIVEVYENIHQSKKCLLIVMECMEGGELFNRIQDRGDQAFTEREASEIMRDIGRAIE--------------HLHSINIAHRDVKPENLLYTSRRSDPVLKLTDFGFAKETLSFNSLATPCYTPYYVAPEVL---GPEKYDKSCDMWSLGVIMYILLCGFPPFYSNHGLAISPGMKKRIRLGQYEFPSPEWTAVSEEAKQVIRKLLKTDPTERMTIAEFMNHPWIN 237
BLAST of Serine/threonine kinase vs. Ensembl Sea Lamprey
Match: RPS6KA3 (ribosomal protein S6 kinase A3 [Source:HGNC Symbol;Acc:HGNC:10432]) HSP 1 Score: 157.147 bits (396), Expect = 1.309e-44 Identity = 86/234 (36.75%), Postives = 131/234 (55.98%), Query Frame = 2
Query: 575 EVEIL-RQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQ-NVTLIKVTDFGLSKFVDGAT-MMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLP--LSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWM 1261
E+EIL R HP ++ L+D D + +++V EL++GGEL D+++ K F E +A +L + ++ YLH + HRDLKP NIL + N +++ DFG +K + ++ T C T N++APEV+ G Y ACDIWSLGV+L+ LAG+ PF D P + +I G++ +W VS A L+K+ML VDP +R T ++L+HPW+
Sbjct: 54 EIEILLRYGQHPNVITLKDVYDDGKSVYLVTELMKGGELLDKILRQKFFSEREASAVLQTIARTVE--------------YLHSQGVVHRDLKPSNILYVDESGNPESLRICDFGFAKQLRAENGLLMTPCYTANFVAPEVLKRQG---YDAACDIWSLGVLLYTMLAGHTPFANGPDDTPGVILTRIGSGRFLLSGGNWDTVSEAAKDLVKKMLHVDPHQRFTAAQVLRHPWI 270
BLAST of Serine/threonine kinase vs. Ensembl Yeast
Match: RAD53 (DNA damage response protein kinase; required for cell-cycle arrest, regulation of copper genes in response to DNA damage; phosphorylates nuclear pores to counteract gene gating, preventing aberrant transitions at forks approaching transcribed genes; activates downstream kinase Dun1p; differentially senses mtDNA depletion, mitochondrial ROS; relocalizes to cytosol under hypoxia; human homolog CHEK2 implicated in breast cancer can complement yeast null mutant [Source:SGD;Acc:S000006074]) HSP 1 Score: 192.2 bits (487), Expect = 1.295e-53 Identity = 148/426 (34.74%), Postives = 207/426 (48.59%), Query Frame = 2
Query: 143 YTVGRGGKIILNSLCPY----FSSLSKIHFEIF-KENQNVILRDLSQNGTYVNDVKVGKDKTHLLQ-----------SNDVISLACQKNKAF-----------VFMHLESTQHLYP---------MVVRNK-------YTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGT----------QEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWM 1261
+T GR N C Y S LS HF+I E+ N++L D+S NGT++N KV K+ LL +D++SL N F + +L++T + +V NK I +G+GA VK A TG+ A+KII K+ + GV RE+E+L++LNHP IV+L+ + E ++VME V GG+L D V + E + + Q++ AIK Y+H ISHRDLKP+NIL+ D V L+K+TDFGL+K + MKT CGT Y+APEVI G T EYS D+WS+G ++++ L G+ PF+ D L KQI G Y + +S A I +LQVDP R T K L HPW+
Sbjct: 66 WTFGR------NPACDYHLGNISRLSNKHFQILLGEDGNLLLNDISTNGTWLNGQKVEKNSNQLLSQGDEITVGVGVESDILSLVIFINDKFKQCLEQNKVDRIRSNLKNTSKIASPGLTSSTASSMVANKTGIFKDFSIIDEVVGQGAFATVKKAIERTTGKTFAVKIISKRKVIG---NMDGVTRELEVLQKLNHPRIVRLKGFYEDTESYYMVMEFVSGGDLMDFVAAHGAVGEDAGREISRQILTAIK--------------YIHSMGISHRDLKPDNILIEQDDPV-LVKITDFGLAKVQGNGSFMKTFCGTLAYVAPEVIRGKDTSVSPDEYEERNEYSSLVDMWSMGCLVYVILTGHLPFSGSTQD-QLYKQIGRGSYHEGPLKDFRISEEARDFIDSLLQVDPNNRSTAAKALNHPWI 466
BLAST of Serine/threonine kinase vs. Ensembl Yeast
Match: DUN1 (Cell-cycle checkpoint S/T protein kinase; required for transient G2/M arrest after DNA damage, damage-induced transcription, and nuclear-to-cytoplasmic redistribution of Rnr2p-Rnr4p after genotoxic stress and iron deprivation; phosphorylates repair protein Rad55p, transcriptional repressor Sml1p, superoxide dismutase, and ribonucleotide reductase inhibitors Crt1p and Dif1p; functions in the Mec1p pathway to regulate dNTP pools and telomere length; postreplicative repair role [Source:SGD;Acc:S000002259]) HSP 1 Score: 181.415 bits (459), Expect = 2.007e-51 Identity = 127/430 (29.53%), Postives = 203/430 (47.21%), Query Frame = 2
Query: 44 FSQNDAEIINSNAWGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSLCPY-FSSLSKIHFEIFKENQNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLESTQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAI-----DTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLA----------------TDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMND 1267
F +N +I+ + G F L Y + G +I+ C + F S +I +++ V S++ +Y ND +V K IS +N A + P+ +KY + + LG G VK A TG++VA+KI + Q E E IL ++ HP IV L D+ + + ++V+E ++ GELF+R++ + +++ L Q++ +K YLH+ NI HRD+KPENILL D+ +K+ DFGL+KF TLCGTP+Y+APEV+ G Y+ D+WS GVIL++CL G+PPF+++ L +QI + +Y+F +W + +HLI +L ++P +R + + L HPW ND
Sbjct: 90 FQRNLINVIDKSRNGTFIN------GNRLVKKDYILKNGDRIVFGKSCSFLFKYASSSSTDIENDDEKVS----SESRSYKNDDEVFKKPQ--------ISATSSQN-ATTSAAIRKLNKTRPVSFFDKYLLGKELGAGHYALVKEAKNKKTGQQVAVKIFHAQQNDDQKKNKQFRE-ETNILMRVQHPNIVNLLDSFVEPISKSQIQKYLVLEKIDDGELFERIVRKTCLRQDESKALFKQLLTGLK--------------YLHEQNIIHRDIKPENILLNITRRENPSQVQLGPWDEDEIDIQVKIADFGLAKFTGEMQFTNTLCGTPSYVAPEVLTKKG---YTSKVDLWSAGVILYVCLCGFPPFSDQLGPPSLKEQILQAKYAFYSPYWDKIDDSVLHLISNLLVLNPDERYNIDEALNHPWFND 482
BLAST of Serine/threonine kinase vs. Ensembl Yeast
Match: CMK1 (Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication [Source:SGD;Acc:S000001910]) HSP 1 Score: 169.474 bits (428), Expect = 1.122e-47 Identity = 107/293 (36.52%), Postives = 164/293 (55.97%), Query Frame = 2
Query: 398 HLYPM--VVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDG-ATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQY--SFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWM 1261
H+ P V + KY +TLG G G V+ A TGE VA+KI+ KKALK + + + E++IL++L+HP IV +D ++ ++ +I+ +L +GGELFDR++ +F E DA +L +++ A+K Y+H NI HRDLKPEN+L + + + V DFG++K + ++ G+ Y+APEV+ +G + CDIWS+GVI + L GY F ER L + T G+Y F +W VS A I + L +DP KR T ++L+ PW+
Sbjct: 25 HVQPASYVNKKKYVFGKTLGAGTFGVVRQAKNTETGEDVAVKILIKKALKGNKVQLEALYDELDILQRLHHPNIVAFKDWFESKDKFYIITQLAKGGELFDRILKKGKFTEEDAVRILVEILSAVK--------------YMHSQNIVHRDLKPENLLYIDKSDESPLVVADFGIAKRLKSDEELLYKPAGSLGYVAPEVLTQDG---HGKPCDIWSIGVITYTLLCGYSAFRAERVQDFLD-ECTTGEYPVKFHRPYWDSVSNKAKQFILKALNLDPSKRPTAAELLEDPWI 299
BLAST of Serine/threonine kinase vs. Ensembl Yeast
Match: CMK2 (Calmodulin-dependent protein kinase; may play a role in stress response, many CA++/calmodulan dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication [Source:SGD;Acc:S000005376]) HSP 1 Score: 164.466 bits (415), Expect = 8.522e-46 Identity = 103/294 (35.03%), Postives = 165/294 (56.12%), Query Frame = 2
Query: 413 VVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGAT-MMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQY--SFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTT 1285
V R Y RTLG G+ G V+ A T E VAIKI+ KKAL+ + + Q + E+ IL++L+HP IV +D ++ ++ +IV +L GGELFDR++S +F EVDA ++ Q++ A++ Y+H N+ HRDLKPEN+L + + + DFG++K + G ++ G+ Y+APEV+ +G + CDIWS+GVI + L GY PF E + ++ T +Y +F +W +S I + L+++P R T ++L PW+ +V+T+
Sbjct: 42 VNRTNYIFGRTLGAGSFGVVRQARKLSTNEDVAIKILLKKALQGNNVQLQMLYEELSILQKLSHPNIVSFKDWFESKDKFYIVTQLATGGELFDRILSRGKFTEVDAVEIIVQILGAVE--------------YMHSKNVVHRDLKPENVLYVDKSENSPLVIADFGIAKQLKGEEDLIYKAAGSLGYVAPEVLTQDG---HGKPCDIWSIGVITYTLLCGYSPFIAESVE-GFMEECTASRYPVTFHMPYWDNISIDVKRFILKALRLNPADRPTATELLDDPWITSKRVETS 317
BLAST of Serine/threonine kinase vs. Ensembl Yeast
Match: SNF1 (AMP-activated S/T protein kinase; forms a complex with Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; regulates nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth and acts as a non-canonical GEF, activating Arf3p during invasive growth; SUMOylation by Mms21p inhibits its function and targets Snf1p for destruction via the Slx5-Slx8 Ub ligase [Source:SGD;Acc:S000002885]) HSP 1 Score: 167.933 bits (424), Expect = 8.835e-46 Identity = 113/281 (40.21%), Postives = 162/281 (57.65%), Query Frame = 2
Query: 428 YTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDD 1270
Y I +TLG+G+ G+VKLAY TG+KVA+KII+KK L + + + +ERE+ LR L HP I+KL D I + + + +V+E G ELFD ++ + E +A+ Q++ A++ Y H + I HRDLKPEN+LL NV K+ DFGLS + +KT CG+PNY APEVI +G D+WS GVIL++ L PF +E + L K I+ G Y+ P+ +S A LIK+ML V+P RI++ +I+Q W D
Sbjct: 55 YQIVKTLGEGSFGKVKLAYHTTTGQKVALKIINKKVLAKSDMQGR-IEREISYLRLLRHPHIIKLYDVIKSKDEIIMVIEYA-GNELFDYIVQRDKMSEQEARRFFQQIISAVE--------------YCHRHKIVHRDLKPENLLLDEHLNV---KIADFGLSNIMTDGNFLKTSCGSPNYAAPEVI--SGKLYAGPEVDVWSCGVILYVMLCRRLPFDDESIPV-LFKNISNGVYTLPKF----LSPGAAGLIKRMLIVNPLNRISIHEIMQDDWFKVD 309
BLAST of Serine/threonine kinase vs. Ensembl Nematostella
Match: EDO44613 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVB0]) HSP 1 Score: 379.793 bits (974), Expect = 5.752e-127 Identity = 206/479 (43.01%), Postives = 292/479 (60.96%), Query Frame = 2
Query: 41 LFSQNDAEIINSNAWGAFFGLLGKFPCVELTDSSYTVGRGG---------KIILNSLCPYFSSLSKIHFEIFKENQN-----VILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLESTQHLY---PMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYPLSEVTKLCQEDLGPCSPSAVGTVNENDNSLSGEPVVKRRK 1426
+ S ++ E ++ WG F L F VEL + Y GRG + LN+ ++ + S H +I ++ N V + DLS NGTYVN K+GK+KTH+L ND ISL+ KNKA++F L +Q P R KYT+S+ LG+GA GEV+LA+ GT K A+K+I K+ ++ ++ EV IL+ LNHPCI+++ D ++ + L+IV+ELVEGGELFDRV+S+K+FEE A++L YQMV+A++ YLH I+HRDLKPENILL +D+N TL+K+TDFG+SK V ++MKTLCGTP+YLAPEV+ G YS A D WSLG +L+ICL GY PF++E ++K I G+Y+FP+ HW VS AI LIK++L VDP KR+T+ ++L+HPW+ D++V A ++MY ++ P+ + N EP KR K
Sbjct: 50 VMSSDEEEDVSPCGWGRLFPLGPGFQLVELIEDEYMFGRGSMNNCRFDATQFKLNA---HYQAFSTKHLKIVRKQNNNNGFDVFITDLSSNGTYVNGEKIGKNKTHVLNHNDEISLSVTKNKAYIFQELGKSQEEVANIPEDFRKKYTLSKLLGRGAFGEVRLAFTKGTCHKFAVKLISKRQFSVSPVSHASIKDEVNILKALNHPCIIEIADVFESPDMLYIVLELVEGGELFDRVVSVKRFEESVAKLLFYQMVVAVE--------------YLHSKGITHRDLKPENILLCSDKNETLLKITDFGVSKMVGEQSLMKTLCGTPSYLAPEVLRSAGLGGYSKAVDCWSLGCMLYICLGGYAPFSDEVEPYDVNKLILAGKYTFPKQHWKCVSDEAIDLIKKLLTVDPSKRLTIQQVLEHPWIKDEEVIEKANRLMYSGKQMP----------PPTVTNVKKRSSNEKEDEPASKREK 501
BLAST of Serine/threonine kinase vs. Ensembl Nematostella
Match: EDO36253 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJ85]) HSP 1 Score: 196.052 bits (497), Expect = 1.142e-58 Identity = 108/282 (38.30%), Postives = 154/282 (54.61%), Query Frame = 2
Query: 416 VRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWM 1261
+ KY + LG GA EV LA TG ++ + + K K + VE E+ IL+++ H I+ L+D D L++VM+LV+GGELFDR++ + E DA L+ Q++ A YLH I HRDLKPEN+L + + I ++DFGLSK + M T CGTP Y+APEV+ Q Y A D WS+GVI +I L GYPPF ++ +D L QI +Y F +W +S A I+ ++++DPK R T + L HPW+
Sbjct: 13 ITKKYDLREVLGTGAFSEVVLAEEKATG---SLFAVKCISKKELKGKEESVENEIAILKKVRHKNIIGLKDIFDNKSHLYLVMDLVQGGELFDRIVEKGNYTEQDASALVQQILEAAD--------------YLHSLGIVHRDLKPENLLYYSPDEDSKIMISDFGLSKIEAQGSFMDTACGTPGYVAPEVL---KQQPYGKAVDCWSIGVITYILLCGYPPFYDD-SDANLFAQIMRAEYEFDSPYWDEISESAKDFIRHLMELDPKLRYTCRQALSHPWI 273
BLAST of Serine/threonine kinase vs. Ensembl Nematostella
Match: EDO42276 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S1X1]) HSP 1 Score: 183.341 bits (464), Expect = 1.129e-53 Identity = 106/293 (36.18%), Postives = 165/293 (56.31%), Query Frame = 2
Query: 416 VRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMK 1294
+ + Y I + LG+GA VKL GT ++ A+KI+ K + + ++ E IL ++NHP I+ +++ +T LF+V+ELV GGELFDRV+ + E DA + ++ A+ +LH N+I HRDLKPEN+L A ++ + +K+ DFGLSK + M T+CGTP Y APEV+ G + Y D+W++GVI +I L G+ PF +E TD + ++I + Y F W VS A L+K+++ D KR+T + L HPW+ + + M+
Sbjct: 14 IEDFYDIGKELGRGASSVVKLCRQKGTNKEFAVKIMKK------TVDKKIIQTETGILFRINHPNIIHMKELFETHTHLFMVLELVTGGELFDRVVEKGYYCEKDAADAIKMILEAVG--------------HLHKNDIVHRDLKPENLLYANEREDSPLKIADFGLSKILPQEVMTSTVCGTPGYCAPEVLKG---ESYDTGVDMWAIGVIAYILLCGFEPFYDE-TDQQMFQRILKCDYDFVSPWWDDVSSGAKDLVKRLITGDKNKRLTAKQALDHPWVKGENARREDME 282
BLAST of Serine/threonine kinase vs. Ensembl Nematostella
Match: EDO33022 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7STC5]) HSP 1 Score: 181.8 bits (460), Expect = 2.634e-53 Identity = 114/288 (39.58%), Postives = 162/288 (56.25%), Query Frame = 2
Query: 410 MVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLA---TDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMN 1264
M + Y I+ +GKGA G+V T E A+K ID + K+Q E +I L+HP IV L D T R+ +V+E + GG+LFD V++ QF E DA + Q++ A++ YL I HRDLKPEN+LL+ +D +IK+TDFG++K + G G+P YLAPE IL G ++A DIW+ GVIL++ L GYPPF E+T+ L I + Y+FP +W VS PA LI++ML VDP+ RIT ++ L+H W++
Sbjct: 1 MKFEDVYEITDFIGKGAFGQVSKCVNLQTREVFAVKEIDFSGSEEDKKKAQN---EAKICGLLDHPSIVHLEDVFFTPRRMLLVLEYLPGGDLFDGVVTRTQFSEKDACACVRQVLKALR--------------YLTRKGIIHRDLKPENLLLSERPSDHRPPIIKLTDFGIAKQLKGN-------GSPLYLAPETILEEG---LTYAVDIWACGVILYLLLVGYPPFWNEQTEF-LLLSILQSNYTFPSPYWDTVSEPAKDLIRKMLVVDPRDRITAVEALKHEWVS 260
BLAST of Serine/threonine kinase vs. Ensembl Nematostella
Match: EDO49898 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RF52]) HSP 1 Score: 178.718 bits (452), Expect = 2.436e-50 Identity = 104/280 (37.14%), Postives = 150/280 (53.57%), Query Frame = 2
Query: 425 KYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATM-MKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWM 1261
KY + LGKGA V+ T K+ + + VERE I R L HP +V+L I +D F+V +LV GGELF+ +++ + + E DA + Q++++++ + H+N I HRDLKPEN+LLA+ + ++K+ DFGL+ VDG + GTP YL+PEV+ + Y D+W+ GVIL+I L GYPPF +E L QI G Y +P W V+ A LI +ML VD KRIT + L+HPW+
Sbjct: 16 KYELKEELGKGAFSTVRKCCHRET--KIEYAVKILDTKNMTQRDVHKVEREARICRHLRHPNVVRLHANIMSDGFYFLVFDLVTGGELFEDIVAREYYSEADASHCIQQVLLSVQ--------------HCHENGIVHRDLKPENLLLASRERGAMVKLADFGLAIEVDGERLGWYGFAGTPGYLSPEVLKKD---PYGKPVDLWACGVILYILLVGYPPFWDEEQQ-KLYSQIKAGTYDYPSPEWDTVTADAKDLIDKMLTVDAPKRITAAEALKHPWI 275
BLAST of Serine/threonine kinase vs. Ensembl Medaka
Match: chek2 (checkpoint kinase 2 [Source:NCBI gene;Acc:101170645]) HSP 1 Score: 326.635 bits (836), Expect = 9.935e-106 Identity = 174/421 (41.33%), Postives = 260/421 (61.76%), Query Frame = 2
Query: 83 WGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSLCPY------------FSSLSKIHFEIFKENQNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLEST-QHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYP 1306
WG + F + Y GR +S C Y F SK HF IF+E+ V + DLS NGT+V+ + VGK+K L +N V+SLA Q+N+ FVF+ L S Q P ++ KY ++R +G G CGEV+LA+ T KVA+K+I+KK + T ++ E E++IL++++HPC++K D T++ FIV+E++EGGELF++V S +Q +E A++ YQM+ A+ YLH N I HRDLKPENILL+++++V LIKVTDF S+ ++ A +M+TLCGTP+YLAPEV T Y A D WSLGV+LF+CL+GY PF E+ + +S+QI G+++ W +S A +++++L VDPK+R+++ + LQHPW+ D ++ TA ++MYP
Sbjct: 76 WGRLLPMAKGFRSHDCIADQYLFGR------DSTCNYVLDDPEERGSRKFRIYSKKHFRIFREDSQVFVEDLSNNGTFVDGLLVGKNKKLPLVNNAVLSLAEQRNRVFVFIDLMSDDQSSLPKDLQEKYLLTRRIGAGVCGEVRLAFERSTCRKVAVKVINKKNFQSEGTATRNAETEIKILQRVDHPCLIKTEDFYQTEDSYFIVLEMMEGGELFNKVKSQQQLKESVAKLYFYQMLCAVH--------------YLHSNGIIHRDLKPENILLSSNEDVCLIKVTDFNQSRILEEAALMRTLCGTPSYLAPEVFTHANTTGYGLAVDAWSLGVLLFVCLSGYAPFHEKFGNHSVSEQIIRGEFTMVPYKWKHISNQAKDMVRKLLVVDPKERMSIDEALQHPWLQDQEMLATAKRLMYP 476
BLAST of Serine/threonine kinase vs. Ensembl Medaka
Match: camk1db (calcium/calmodulin-dependent protein kinase type 1D [Source:NCBI gene;Acc:101175563]) HSP 1 Score: 192.2 bits (487), Expect = 1.511e-55 Identity = 115/285 (40.35%), Postives = 162/285 (56.84%), Query Frame = 2
Query: 416 VRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDD 1270
V+ + LG GA EV +A TG+ VAIK I KKALK T +E E+ +LR++ H IV L D ++ L+++M+LV GGELFDR++ + E+DA L+ Q++ D + YLH I HRDLKPEN+L + + + I ++DFGLSK +M T CGTP Y+APEV+ + YS A D WS+GVI +I L GYPPF +E D L +QI + Y F +W +S A I +++ DP+KR T + L+HPW+ D
Sbjct: 20 VKKIFDFKEVLGTGAFSEVVMAREKATGKMVAIKCIPKKALKGKET---SIENEIAVLRKIKHENIVALEDIYESSNHLYLIMQLVSGGELFDRIVEKGFYTEMDASRLIRQVL--------------DAVNYLHSMGIVHRDLKPENLLYFSPHDDSKIMISDFGLSKMEGTGGVMATACGTPGYVAPEVL---AQKPYSKAVDCWSIGVIAYILLCGYPPFYDE-NDSKLFEQILKADYEFDAPYWDDISDSAKDFISCLMEKDPEKRFTCDQALEHPWIAGD 283
BLAST of Serine/threonine kinase vs. Ensembl Medaka
Match: camk1b (calcium/calmodulin-dependent protein kinase type 1 [Source:NCBI gene;Acc:101170017]) HSP 1 Score: 190.274 bits (482), Expect = 3.565e-55 Identity = 116/285 (40.70%), Postives = 159/285 (55.79%), Query Frame = 2
Query: 416 VRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDD 1270
V+ +Y LG GA EV LA T + VAIK I KKAL K +E E+ +L ++ HP IV L D ++ L+++M+LV GGELFDR+I + E DA L+ Q++ A+K YLHD I HRDLKPEN+L + + I ++DFGLSK ++M T CGTP Y+APEV+ + YS A D WS+GVI +I L GYPPF +E D L +QI + +Y F +W +S A I +++ D R T + L HPW+ D
Sbjct: 17 VKEQYDFKEILGTGAFSEVVLAEEKRTHKLVAIKCIPKKAL---EGKENSIENEIAVLHKIKHPNIVSLEDIFESKSHLYLIMQLVSGGELFDRIIEKGFYTEKDASKLIQQILDAVK--------------YLHDMGIVHRDLKPENLLYYSMDEDSKIMISDFGLSKIEGSGSVMSTACGTPGYVAPEVL---AQKPYSKAVDCWSIGVISYILLCGYPPFYDE-NDAKLFEQILKAEYEFDSPYWDDISDSAKDFIVHLMEKDSNTRYTCDQALLHPWIAGD 280
BLAST of Serine/threonine kinase vs. Ensembl Medaka
Match: camk1b (calcium/calmodulin-dependent protein kinase type 1 [Source:NCBI gene;Acc:101170017]) HSP 1 Score: 190.66 bits (483), Expect = 3.679e-55 Identity = 116/285 (40.70%), Postives = 159/285 (55.79%), Query Frame = 2
Query: 416 VRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDD 1270
V+ +Y LG GA EV LA T + VAIK I KKAL K +E E+ +L ++ HP IV L D ++ L+++M+LV GGELFDR+I + E DA L+ Q++ A+K YLHD I HRDLKPEN+L + + I ++DFGLSK ++M T CGTP Y+APEV+ + YS A D WS+GVI +I L GYPPF +E D L +QI + +Y F +W +S A I +++ D R T + L HPW+ D
Sbjct: 30 VKEQYDFKEILGTGAFSEVVLAEEKRTHKLVAIKCIPKKAL---EGKENSIENEIAVLHKIKHPNIVSLEDIFESKSHLYLIMQLVSGGELFDRIIEKGFYTEKDASKLIQQILDAVK--------------YLHDMGIVHRDLKPENLLYYSMDEDSKIMISDFGLSKIEGSGSVMSTACGTPGYVAPEVL---AQKPYSKAVDCWSIGVISYILLCGYPPFYDE-NDAKLFEQILKAEYEFDSPYWDDISDSAKDFIVHLMEKDSNTRYTCDQALLHPWIAGD 293
BLAST of Serine/threonine kinase vs. Ensembl Medaka
Match: camk1b (calcium/calmodulin-dependent protein kinase type 1 [Source:NCBI gene;Acc:101170017]) HSP 1 Score: 190.66 bits (483), Expect = 3.948e-55 Identity = 116/285 (40.70%), Postives = 159/285 (55.79%), Query Frame = 2
Query: 416 VRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDD 1270
V+ +Y LG GA EV LA T + VAIK I KKAL K +E E+ +L ++ HP IV L D ++ L+++M+LV GGELFDR+I + E DA L+ Q++ A+K YLHD I HRDLKPEN+L + + I ++DFGLSK ++M T CGTP Y+APEV+ + YS A D WS+GVI +I L GYPPF +E D L +QI + +Y F +W +S A I +++ D R T + L HPW+ D
Sbjct: 35 VKEQYDFKEILGTGAFSEVVLAEEKRTHKLVAIKCIPKKAL---EGKENSIENEIAVLHKIKHPNIVSLEDIFESKSHLYLIMQLVSGGELFDRIIEKGFYTEKDASKLIQQILDAVK--------------YLHDMGIVHRDLKPENLLYYSMDEDSKIMISDFGLSKIEGSGSVMSTACGTPGYVAPEVL---AQKPYSKAVDCWSIGVISYILLCGYPPFYDE-NDAKLFEQILKAEYEFDSPYWDDISDSAKDFIVHLMEKDSNTRYTCDQALLHPWIAGD 298
BLAST of Serine/threonine kinase vs. Planmine SMEST
Match: SMESG000002981.1 (SMESG000002981.1) HSP 1 Score: 912.909 bits (2358), Expect = 0.000e+0 Identity = 459/473 (97.04%), Postives = 459/473 (97.04%), Query Frame = 2
Query: 11 MEDSQFNTQELFSQNDAEIINSNAWGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSLCPYFSSLSKIHFEIFKENQNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLESTQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEXXXXXXXXXXXXXXXSTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYPLSEVTKLCQEDLGPCSPSAVGTVNENDNSLSGEPVVKRRKF 1429
MEDSQFNTQELFSQNDAEIINSNAWGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSLCPYFSSLSKIHFEIFKENQNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLESTQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEKVAIKIIDKKALKLASTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIK YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYPLSEVTKLCQEDLGPCSPSAVGTVNENDNSLSGEPVVKRRKF
Sbjct: 1 MEDSQFNTQELFSQNDAEIINSNAWGAFFGLLGKFPCVELTDSSYTVGRGGKIILNSLCPYFSSLSKIHFEIFKENQNVILRDLSQNGTYVNDVKVGKDKTHLLQSNDVISLACQKNKAFVFMHLESTQHLYPMVVRNKYTISRTLGKGACGEVKLAYVNGTGEKVAIKIIDKKALKLASTKSQGVEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIK--------------YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMKIMYPLSEVTKLCQEDLGPCSPSAVGTVNENDNSLSGEPVVKRRKF 459
BLAST of Serine/threonine kinase vs. Planmine SMEST
Match: SMESG000078937.1 (SMESG000078937.1) HSP 1 Score: 198.749 bits (504), Expect = 6.209e-59 Identity = 108/292 (36.99%), Postives = 159/292 (54.45%), Query Frame = 2
Query: 422 NKYTISRTLGKGACGEVKLA-------YVNGTGEXXXXXXXXXXXXXXXSTKSQGVE----REVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMN 1264
++Y LG GA EV+LA +V K +A ++Q E E+ +LR+L H IV+L D +++D+ ++VMELV GGELFDR++ + E DA L+ Q++ A Y+H+ + HRDLKPEN+L + + + I ++DFGLSK D + M T CGTP Y+APEV+ N + Y D W++GVI +I L GYPPF +E D L +QI E QY F +W +S A IK +++ +PKKR + + L HPW++
Sbjct: 8 SRYQFKEVLGTGAFSEVRLAEDTEKRSFVAVKCIKKKAITASSRASNKDKQEAQAREDSLKNEISVLRKLKHENIVQLLDVLESDDTYYLVMELVTGGELFDRIVEKGSYTEKDASNLIRQVLEATD--------------YMHEQGVVHRDLKPENLLYYSPDDDSKIMISDFGLSKMDDADSAMATACGTPGYVAPEVLEVNSGRSYGKEVDCWAVGVIAYILLCGYPPFYDE-NDQELFRQIKEAQYEFDSPYWDEISDSAKDFIKHLMEKNPKKRYSCKESLAHPWIS 284
BLAST of Serine/threonine kinase vs. Planmine SMEST
Match: SMESG000028730.1 (SMESG000028730.1) HSP 1 Score: 179.489 bits (454), Expect = 1.467e-51 Identity = 100/248 (40.32%), Postives = 148/248 (59.68%), Query Frame = 2
Query: 560 QGVEREVEI-LRQLNHPCIVKLRDAIDTDE------RLFIVMELVEGGELFDRV--ISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLS----KQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMN 1264
+ REV++ R N P IV + D + + L +VME +EGGELF+R+ + K F E +A ++Y++ AIK YLHD NI+HRDLKPEN+L + +K+TDFG +K V +++T C TP Y+APEV+ G ++Y +CD+WSLGVI +I L GYPPF + P+S K+I G+Y+FP+ W+ VS A LI +L DP+KR+T+ ++++HPW+N
Sbjct: 49 EKARREVDLHWRANNCPHIVNIYDIYENNNLVTKKNHLLVVMECMEGGELFNRIQQRADKAFTEREAAQIVYEISSAIK--------------YLHDMNIAHRDLKPENLLYTEAGSNGKLKLTDFGFAKEVHSVKILQTPCYTPYYVAPEVL---GPEKYDKSCDLWSLGVITYILLCGYPPFFSQNGQ-PISPGMKKRIRNGEYTFPKSEWANVSSNAKDLITNLLLTDPEKRLTINQVMEHPWIN 278
BLAST of Serine/threonine kinase vs. Planmine SMEST
Match: SMESG000026357.1 (SMESG000026357.1) HSP 1 Score: 174.481 bits (441), Expect = 3.308e-50 Identity = 93/233 (39.91%), Postives = 142/233 (60.94%), Query Frame = 2
Query: 572 REVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQE--YSFACDIWSLGVILFICLAGYPPFTEERTDL-PLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWM 1261
RE I++ L+HP I+K D ID L+IVM+L EGGEL+DR++ ++F E +A ++L Q++ +K Y+H++ HRD+KPEN+L T + + + +TDFGLS+ ++ M T CGT +Y APE +L N + Y+ D WS+GV+ +I L GYPPF D+ + KQI +G+Y F + HW+ VSR LI +L+V P++RI+L L+HP+
Sbjct: 70 REASIMQALDHPNIIKFYDLIDDGIDLYIVMQLAEGGELYDRILKRQKFTENEAVIVLKQIICGVK--------------YMHEHGYVHRDIKPENVLFETINDDSRLMITDFGLSREINEKMM--TNCGTIDYSAPE-LLKNRLNKLGYNEKVDHWSIGVVAYILLCGYPPFYSMCKDVQEIKKQIMDGKYYFHKPHWNTVSRSCKRLISSLLKVKPEERISLDSCLEHPFF 285
BLAST of Serine/threonine kinase vs. Planmine SMEST
Match: SMESG000042100.1 (SMESG000042100.1) HSP 1 Score: 175.637 bits (444), Expect = 4.883e-50 Identity = 93/243 (38.27%), Postives = 142/243 (58.44%), Query Frame = 2
Query: 566 VEREVEILRQLNHPCIVKLRDAIDTDERLFIVMELVEGGELFDRVISIKQFEEVDAQVLLYQMVMAIKVSNNGYRASNDTI*YLHDNNISHRDLKPENILLATDQNVTLIKVTDFGLSKFVDGATMMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEERTDLPLSKQITEGQYSFPEVHWSGVSRPAIHLIKQMLQVDPKKRITLIKILQHPWMNDDKVKTTAMK 1294
++ EV +L +L HP I++L + ++ + +V+E V GGELFDR++S + E DA + +M+ A+ YLH+N+I HRDLKPEN+L + + L K+ DFG++K ++ T+CGTP Y APE++LG Y D+WS+GVI +I L GY PF E+ D + K I +G YSF W VS A +I ++L VDP KR+T + L H W+ + KT++++
Sbjct: 59 IKSEVGVLLKLTHPNIIRLYEVYESATDVHMVLEYVSGGELFDRIVSRGFYSEKDASRCVREMLQAVS--------------YLHENDIIHRDLKPENLLYESQEESNL-KLADFGIAKILEDDVHTMTVCGTPGYCAPEILLG---VSYGKPVDLWSIGVITYILLCGYEPFYNEK-DNQMFKNIIKGIYSFSSPWWDNVSLNAKDIISKLLVVDPHKRLTANQALNHVWVKGEGAKTSSLE 282 The following BLAST results are available for this feature:
BLAST of Serine/threonine kinase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Serine/threonine kinase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Serine/threonine kinase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Serine/threonine kinase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Serine/threonine kinase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Serine/threonine kinase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Serine/threonine kinase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Serine/threonine kinase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Serine/threonine kinase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Serine/threonine kinase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Serine/threonine kinase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Serine/threonine kinase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Serine/threonine kinase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Serine/threonine kinase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30026997 ID=SMED30026997|Name=Serine/threonine kinase|organism=Schmidtea mediterranea sexual|type=transcript|length=1543bpback to top protein sequence of SMED30026997-orf-1 >SMED30026997-orf-1 ID=SMED30026997-orf-1|Name=SMED30026997-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=164bp MMKTLCGTPNYLAPEVILGNGTQEYSFACDIWSLGVILFICLAGYPPFTEback to top protein sequence of SMED30026997-orf-2 >SMED30026997-orf-2 ID=SMED30026997-orf-2|Name=SMED30026997-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=270bp LKSMEDSQFNTQELFSQNDAEIINSNAWGAFFGLLGKFPCVELTDSSYTVback to top Annotated Terms
The following terms have been associated with this transcript:
|
